Lsi08G015290 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G015290
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	DAR GTPase 2, mitochondrial
Location	chr08: 23339499 .. 23343517 (+)
RNA-Seq Expression	Lsi08G015290
Synteny	Lsi08G015290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGGCACAAATCAGACCGAACCGAACCATGCACACCTCCTGACAATCCCTCTTCCAACCCGCGAAAGACCATATAACCTTTCGAGTCTAAATGTCGATTTGAAGTTTTGGAGCCAAAATGGCCGCGGCAACGCTGATGAGAAAAATAGGTACGGCTATCAAGGGAGAGGCTACTGGAAACCGCATCTCCAGCGGATGGTACGATCCTCACATGGCGGCCGCTTCTCGCGCCGTCGCGGAACGAATTCCACTGGTTGATTTTGTTCTTGAAGTCAGAGATGCTAGGGTATGCAAGCTCCTACTATTGTTTACTCCCTCTCATTGCATATTGTTTAATTTCGTACTGGCACTTTTAAGTGTTCGTTGATAGCCTGTTCATAGTTTGCTCAATTCGTAGCTTTAGTTTATTTTTCGTTACCGCTTTTGTTTCAATCTTTTCGGAAACCTGGATTTGTTTATTCATATATGTGGTGGTTCGCTTCGTCTTCGATAAAATAATCTAGGAACAATGATGTTGTAACGAAGATGAAGGATTGCGTTTATCAAACTGGAGGGAAAGAATCACTAAGAATGTTTGTTCATTCCTCTCCGTGCTATTCCTTGTTTTGTTTTGTTTTCAGATTCCGATGTCATCTGAATATGAAATCACGAAAAATTATCCACCATCGTCGAAACGAATAATTGTACTGAACAAGTCGGACCTGGCGGATCGATCACAAACAGAGGTTCGGATCTTCTGTAATTCCATGATTCTCAGTCATTTTAGCATTTTCAGTCATTTTAGCTTAGCTTTTACTGCTGGAGTTACTTTGAAGTCTTTCTGGATTTCATAGGTCACCAATGGAGCTTATGTGAATTGCTACTGATGTCTAGCTCCTAGTTTCTTGATTGAACATTATAATGAGAATTTTGTGGGATATTTAGGGAGAGAAGACAGAAGAGCATAAGATATAAATAGAATATCTGTGGGATGGAATTTATTGTTTGCCCTCGGCCCAGGGGTTTTATAATTTTCCATTGTTATGACTTTCACATAGCTAAGAGTTTTTACCCTTTTTGTTTTAGAAGGATCTCCGGTCACCTTACTTGACCTTGCAATTATATTTGGATAGAAATGCTCATTTGTTTCTTATAAAAAGAAAGAATTACATGACAGTATGAAAGAGGATTTTTACTTAGCTATCATTTACGAGGTAATTTATTCTTTTACAATTTGAATCATATTCAAAATGCTTTATCAGTCAGTATCTATTGTTTCACATGTTTATACTTTGCTCTATTAATAGGTCTGGACTCGGTATTTTGAAGAGCACAACTGCATCTCTTATGGTGTTAATTCCCATAATAAAGAGAATATTAGGGAGGTAAAGAACTTAGATGTAATCTTTTTGTGTTCATTATGCCATCTTGTTCGCGCTACTTATCTTCCTCTGTCTGGCTTAGAATGCTGGTGGTAGTAATTGTAACATGTTTTTGGCAGTATATGCATTTTTTCCTTTAACATTGTTTTTCATGCAAAATTAAGTGCCAACTAAATGTAATTTATATATCCCACTTTGCGATCGATACTTTCTCTCCATGGGACAATCCGTTAGTTGTTTAATCTCTTGAGCTGACCTTATTTACTTTGTCTAATAGTGATTTCTGACGATGGTTCACTTCATACAGTTCTTGAATTTTCTTCAAGCTAGAGTTAGAGAATTGAAGAAGAGCGGTCATTCCAGCCATGCCACCACGATGATGCTGGTTGGAATTCCAAATGTTGGTAAGTCTGCCCTTGCCAACTCCCTGCATCAAATTGGGAGAATTAGTGCTGCAGGTACATGTCTTAAATTGCACCGAGTTTGAGTTATCTTGAGCAGTTGATCCTGGAATTGTTTGGATGAATAATGATTGTTATTTATCTAATTACAAAGAATTTCTATTTGTGACATTTTGAAGAACTGCTCACCTCACAGAAAAAGGGAAGTTGAAGCATGCTATAGCGAGTTCACAGCCTGGTGAAACAAAAAATATCAGCAGCTTGAAGGTGTGTCATCTGTCTCCACGACATTGTAGTATGTTGTGAAGTTGTCTCACTATCCTGGGGTTACTGACTCAGCTGTTGAGGTGTTTATTTAAATCTTTACATTAGCATATTTCGGGTTGAACAAGGGTTAAAAAGTTGTTCTACTTTCTAATTGTGGGTAGCCATGAAATGATCCCAAATCGAACTATTGTCATGAATATCATCTATCCCTTTAGGAGCAAAGATAATGTGTACTTTTGTTTTGTATAAGAATTTGAATTTTCTTTTTAAGTTGCTTTGAATTACAGATAGCTAGTCATCCCAATATCTATGTATTAGACACCCCAGGCATTTTTCCTCCCAAGATTCACAACATTGAGGTCTGTTCCAAGCTTGCTTTAACAGGTATAATGTTATGAAGCCAATTGATCTATCTTTCTTGCGTAAATTATTTAAGCCAGTAAAAGATAGTGAATCACGAATATGAAGTGATAAACATATTTGCGTTTTCCTTTTTAATCTAGTGCAACATTTGTGCTAGTTCAGAGACTGTAGAGATGAATGAGAATCTTGAACTGTTAACTTTTTTTTGTTTTGCCTCCAAACTGTAGGGGCAATTAGAGACATCTTAGTTGGGGAACATGTAATTGTCCAGTACCTTTTAACTATTGTCAACTCAAGTGATAAATATAAGAAGTGGGCAAATTTGTCTGCCACGGACAACTCGTTGGAGTGTTCAACTAGCTCAAGTCTAGAGAAACAGAAAAGGAGGTATCCGTCTGATCACACGCAGGTATTAGGCATCATATGGTTCTCCTTTTCCTCTAAAAGCATTGTATGTATATTGTTCTCGTTTTCATCCGTCTGATCACACGCAGTTATAAGGCATTTTATGGTTCTCTTTCTTCATCTAAAATCATTATGTATGTATACTGAACCTAATTCATGCATGTTTAGTTGGCAACTAAAGTCTTTGTAGAATTCTTTTAGTTTTCACATTTTCTCCATCTATTATTAGAGAGAGGACACGTGGTTGATTCCTTTACTAAAGTAGTTTATTAATTTTTAATTTTGGAGGCAACCTGTCACATGACACCAGTTATATGCTTATTATCTGTAATAGAAGTAACTGGTAAGTTAAAAAGTTTTTAGTTTCTTGCCGATGCAAGATCGGTACAAGCCTACATTTGTCAATTTACCATGGGATGTAAAACTCCCGGTGGGATAAATAGCCTGACAATAGGATTAAGAACATACTACAAAACTGTTTGGATTGACTTTAGAAGTGCTTAAAATATATACACGAAAATTATTTTTCTTTCATTTAAGTACTTAAAAAGTCAATCTAAACAGACCCTACATTAATGGATGGAAGTGTTAGTAAAATGCCAGTTGGGTGAAATGATATAGTTTTCTGTGCATGCAGGACATTATTGTTAATGGAGTTCGTAGAATTCTCTTCGAGACTATATCATCTTTTGTTGGTAATTTGGAGGATGAAAAGGACATGGGAAATCTTATTGAGACACAATTGCATGCTCTGCACAAAGCTTTGCACGTTCCTATGGATTTTTGCAGTGATGCTTCTATCAAGGTTGCTTCTAAACTTTTGAATCTTTATCGTACTGGGAGGCTGGGTTGTTACACACTTGATTCACTGCCACCTGTTAATACTTACTGAGCAAAATCTTGTAAATTATCTACTGTTAAAGTCATTATAACTTTAACTATTTCTCCAATCTCACTATTCTTAACCGTATGGTTTTGAGAAAGTTAGAATTTAGTCCATATGATTTATAATTATAATTTAGTCCCTATAATTTGATAAAACCATCATAAATAGTCTATACGGTTCAAATTATAGGGACTATTTACGAGGTTTTATCAAACCTTAAGGGCTAAATTCTAACTTTTGAATTCATACGGATTAAATTCTAACTTTATAACTATATGGGTCAAATTTGTAATTAACTCCCTTTTTTTTAAATTTCAAAACCTTAAAATTTACAT

mRNA sequence

ATTTGGCACAAATCAGACCGAACCGAACCATGCACACCTCCTGACAATCCCTCTTCCAACCCGCGAAAGACCATATAACCTTTCGAGTCTAAATGTCGATTTGAAGTTTTGGAGCCAAAATGGCCGCGGCAACGCTGATGAGAAAAATAGGTACGGCTATCAAGGGAGAGGCTACTGGAAACCGCATCTCCAGCGGATGGTACGATCCTCACATGGCGGCCGCTTCTCGCGCCGTCGCGGAACGAATTCCACTGGTTGATTTTGTTCTTGAAGTCAGAGATGCTAGGATTCCGATGTCATCTGAATATGAAATCACGAAAAATTATCCACCATCGTCGAAACGAATAATTGTACTGAACAAGTCGGACCTGGCGGATCGATCACAAACAGAGGTCTGGACTCGGTATTTTGAAGAGCACAACTGCATCTCTTATGGTGTTAATTCCCATAATAAAGAGAATATTAGGGAGTTCTTGAATTTTCTTCAAGCTAGAGTTAGAGAATTGAAGAAGAGCGGTCATTCCAGCCATGCCACCACGATGATGCTGGTTGGAATTCCAAATGTTGGTAAGTCTGCCCTTGCCAACTCCCTGCATCAAATTGGGAGAATTAGTGCTGCAGAAAAAGGGAAGTTGAAGCATGCTATAGCGAGTTCACAGCCTGGTGAAACAAAAAATATCAGCAGCTTGAAGATAGCTAGTCATCCCAATATCTATGTATTAGACACCCCAGGCATTTTTCCTCCCAAGATTCACAACATTGAGGTCTGTTCCAAGCTTGCTTTAACAGGGGCAATTAGAGACATCTTAGTTGGGGAACATGTAATTGTCCAGTACCTTTTAACTATTGTCAACTCAAGTGATAAATATAAGAAGTGGGCAAATTTGTCTGCCACGGACAACTCGTTGGAGTGTTCAACTAGCTCAAGTCTAGAGAAACAGAAAAGGAGGTATCCGTCTGATCACACGCAGGACATTATTGTTAATGGAGTTCGTAGAATTCTCTTCGAGACTATATCATCTTTTGTTGGTAATTTGGAGGATGAAAAGGACATGGGAAATCTTATTGAGACACAATTGCATGCTCTGCACAAAGCTTTGCACGTTCCTATGGATTTTTGCAGTGATGCTTCTATCAAGGTTGCTTCTAAACTTTTGAATCTTTATCGTACTGGGAGGCTGGGTTGTTACACACTTGATTCACTGCCACCTGTTAATACTTACTGAGCAAAATCTTGTAAATTATCTACTGTTAAAGTCATTATAACTTTAACTATTTCTCCAATCTCACTATTCTTAACCGTATGGTTTTGAGAAAGTTAGAATTTAGTCCATATGATTTATAATTATAATTTAGTCCCTATAATTTGATAAAACCATCATAAATAGTCTATACGGTTCAAATTATAGGGACTATTTACGAGGTTTTATCAAACCTTAAGGGCTAAATTCTAACTTTTGAATTCATACGGATTAAATTCTAACTTTATAACTATATGGGTCAAATTTGTAATTAACTCCCTTTTTTTTAAATTTCAAAACCTTAAAATTTACAT

Coding sequence (CDS)

ATGGCCGCGGCAACGCTGATGAGAAAAATAGGTACGGCTATCAAGGGAGAGGCTACTGGAAACCGCATCTCCAGCGGATGGTACGATCCTCACATGGCGGCCGCTTCTCGCGCCGTCGCGGAACGAATTCCACTGGTTGATTTTGTTCTTGAAGTCAGAGATGCTAGGATTCCGATGTCATCTGAATATGAAATCACGAAAAATTATCCACCATCGTCGAAACGAATAATTGTACTGAACAAGTCGGACCTGGCGGATCGATCACAAACAGAGGTCTGGACTCGGTATTTTGAAGAGCACAACTGCATCTCTTATGGTGTTAATTCCCATAATAAAGAGAATATTAGGGAGTTCTTGAATTTTCTTCAAGCTAGAGTTAGAGAATTGAAGAAGAGCGGTCATTCCAGCCATGCCACCACGATGATGCTGGTTGGAATTCCAAATGTTGGTAAGTCTGCCCTTGCCAACTCCCTGCATCAAATTGGGAGAATTAGTGCTGCAGAAAAAGGGAAGTTGAAGCATGCTATAGCGAGTTCACAGCCTGGTGAAACAAAAAATATCAGCAGCTTGAAGATAGCTAGTCATCCCAATATCTATGTATTAGACACCCCAGGCATTTTTCCTCCCAAGATTCACAACATTGAGGTCTGTTCCAAGCTTGCTTTAACAGGGGCAATTAGAGACATCTTAGTTGGGGAACATGTAATTGTCCAGTACCTTTTAACTATTGTCAACTCAAGTGATAAATATAAGAAGTGGGCAAATTTGTCTGCCACGGACAACTCGTTGGAGTGTTCAACTAGCTCAAGTCTAGAGAAACAGAAAAGGAGGTATCCGTCTGATCACACGCAGGACATTATTGTTAATGGAGTTCGTAGAATTCTCTTCGAGACTATATCATCTTTTGTTGGTAATTTGGAGGATGAAAAGGACATGGGAAATCTTATTGAGACACAATTGCATGCTCTGCACAAAGCTTTGCACGTTCCTATGGATTTTTGCAGTGATGCTTCTATCAAGGTTGCTTCTAAACTTTTGAATCTTTATCGTACTGGGAGGCTGGGTTGTTACACACTTGATTCACTGCCACCTGTTAATACTTACTGA

Protein sequence

MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLNFLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYLLTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETISSFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLDSLPPVNTY

Homology

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BLAST of Lsi08G015290 vs. ExPASy Swiss-Prot
Match: O82497 (DAR GTPase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DGP2 PE=3 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 6.0e-120
Identity = 227/360 (63.06%), Postives = 275/360 (76.39%), Query Frame = 0


Query: 6   LMRKIGTA-IKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYE 65
           + R+IG A IK     NR    WY PHMAAA RA++ERIPLVDFVLE+RDARIP+SSEYE
Sbjct: 9   IAREIGDAVIKASRNPNR---RWYGPHMAAAVRAISERIPLVDFVLEIRDARIPLSSEYE 68

Query: 66  ITKNYPP-SSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLNFLQ 125
           + + + P  SKRIIVLNK +LAD  + +    YFEE N +SY VNSHNK+ +++ LNFLQ
Sbjct: 69  LLRKFSPLPSKRIIVLNKMELADPLELKKCIDYFEERNYLSYAVNSHNKDCVKQLLNFLQ 128

Query: 126 ARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGE 185
           ++VREL K+GHS H TTMML+GIPNVGKSAL+NSLH IGRISAAEKGKLKH   SSQPG+
Sbjct: 129 SQVRELHKAGHSGHTTTMMLLGIPNVGKSALSNSLHHIGRISAAEKGKLKHTTVSSQPGD 188

Query: 186 TKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYLLTI 245
           TK+I SLKI SHPN+YVLDTPGIFPP +++ E+C+KLALTGAI D +VGE  + +  LTI
Sbjct: 189 TKDIMSLKIGSHPNVYVLDTPGIFPPNLYDAEICAKLALTGAIPDDIVGELKLARLFLTI 248

Query: 246 VNSSDKYKKWANLSATDN---SLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 305
           +NSS +YKKWA L  + +   SL   +S S  K KR+Y +DHTQD IV  VRR+L+ETIS
Sbjct: 249 LNSSHEYKKWAKLCKSQDLTESLSDESSKSDAKHKRQYATDHTQDFIVYDVRRVLYETIS 308

Query: 306 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 361
           +F GNLEDE  MGNLIETQ  AL   L VP +    A ++VASK+LNLYRTGRLG YTL+
Sbjct: 309 AFDGNLEDEISMGNLIETQFAALRSVLRVPEEASEFADLRVASKILNLYRTGRLGHYTLE 365

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BLAST of Lsi08G015290 vs. ExPASy Swiss-Prot
Match: Q8L607 (Short integuments 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SIN2 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 9.7e-62
Identity = 139/352 (39.49%), Postives = 203/352 (57.67%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYEITKNYPPSSKRIIVLNKSDLAD 86
           W+  HMAAA+RA+  R+ L D V+EVRDARIP+SS  E  ++   + +RII LNK DLA+
Sbjct: 28  WFPGHMAAATRAIRNRLKLSDLVIEVRDARIPLSSANEDLQSQMSAKRRIIALNKKDLAN 87

Query: 87  RSQTEVWTRYFEEHNCISYGVNSHNKENIREFLNFLQARVRELKKSGHSSHATTMMLVGI 146
            +    WTR+FE        +N+H++ ++ + L+ ++ +++E+           +M+VG+
Sbjct: 88  PNVLNKWTRHFESSKQDCIAINAHSRSSVMKLLDLVELKLKEVIA---REPTLLVMVVGV 147

Query: 147 PNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGETKNISSLKIASHPNIYVLDTPGI 206
           PNVGKSAL NS+HQI       + +LK A     PG T++I+  KIA  P+IYVLD+PG+
Sbjct: 148 PNVGKSALINSIHQIAAARFPVQERLKRATVGPLPGVTQDIAGFKIAHRPSIYVLDSPGV 207

Query: 207 FPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYLLTIVNSSDKYKKWANLSATDNS---L 266
             P I +IE   KLAL+G+++D +VGE  I QY L I+N       W  L    N     
Sbjct: 208 LVPSIPDIETGLKLALSGSVKDSVVGEERIAQYFLAILNIRGTPLHWKYLVEGINEGPHA 267

Query: 267 ECSTSSS-----LEKQKRRYPSD---HTQDIIVNGVRRILFETISSFVGNLEDEKDMGNL 326
           +C    S     L  Q+ + P     H    +++ V+R L+ T+S F G+ EDE D+  L
Sbjct: 268 DCIDKPSYNLKDLRHQRTKQPDSSALHYVGDMISEVQRSLYITLSEFDGDTEDENDLECL 327

Query: 327 IETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLDSLPPVNT 368
           IE Q   L KAL +P    S+A + V+ K L L+RTGRLG + LD +P   T
Sbjct: 328 IEQQFEVLQKALKIPHK-ASEARLMVSKKFLTLFRTGRLGPFILDDVPETET 375

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BLAST of Lsi08G015290 vs. ExPASy Swiss-Prot
Match: Q65JP4 (Ribosome biogenesis GTPase A OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=rbgA PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.3e-26
Identity = 88/285 (30.88%), Postives = 136/285 (47.72%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYEITKNYPPSSKRIIVLNKSDLAD 86
           W+  HMA A R V E++ L+D V E+ DARIPMSS   + ++   +  RI++LNK+D AD
Sbjct: 5   WFPGHMAKARREVTEKLKLIDIVYELVDARIPMSSRNPMIEDILKNKPRIMLLNKADKAD 64

Query: 87  RSQTEVWTRYFEEHNCISYGVNSHNKENIREFL----NFLQARVRELKKSGHSSHATTMM 146
            S T+ W ++FE+    +  +NS N + + + L      L+ +  ++K  G    A   +
Sbjct: 65  SSVTKAWKQHFEKDGIPTLAINSVNGQGLNQILPASKELLKEKFDKMKAKGVKPRAIRAL 124

Query: 147 LVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGETKNISSLKIASHPNIYVLD 206
           +VGIPNVGKS L N L            K   A    +PG T     +K+     + +LD
Sbjct: 125 IVGIPNVGKSTLINRL-----------AKKNIAKTGDRPGVTTAQQWVKVGK--ELELLD 184

Query: 207 TPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYLLTIV--NSSDKYKKWANLSATD 266
           TPGI  PK  +  V  +LA TGAI+D ++    +  Y L  +  N  ++ KK  +L    
Sbjct: 185 TPGILWPKFEDELVGLRLAATGAIKDSIINLQDVAVYGLRFLEENYPERLKKRYDLEEIP 244

Query: 267 NSL-----ECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 301
             +     E                D T ++I+  +R   F  +S
Sbjct: 245 EEIAALFDEIGKKRGCLMAGGEINYDKTTEVIIRDIRTEKFGPLS 276

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BLAST of Lsi08G015290 vs. ExPASy Swiss-Prot
Match: E0TTS5 (Ribosome biogenesis GTPase A OS=Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) OX=655816 GN=rbgA PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.8e-26
Identity = 73/209 (34.93%), Postives = 111/209 (53.11%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYEITKNYPPSSKRIIVLNKSDLAD 86
           W+  HMA A R V E++ L+D V E+ DARIPMSS   + ++   +  RI++LNK+D AD
Sbjct: 5   WFPGHMAKARREVTEKLKLIDIVYELVDARIPMSSRNPMIEDILKNKPRIMLLNKADKAD 64

Query: 87  RSQTEVWTRYFEEHNCISYGVNSHNKENIREFL----NFLQARVRELKKSGHSSHATTMM 146
            + T+ W  +FE     S  +NS N + + + +      LQ +   ++  G    A   +
Sbjct: 65  AAVTQQWKEHFENQGIRSLSINSVNGQGLNQIVPASKEILQEKFDRMRAKGVKPRAIRAL 124

Query: 147 LVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGETKNISSLKIASHPNIYVLD 206
           ++GIPNVGKS L N L            K   A    +PG T +   +K+     + +LD
Sbjct: 125 IIGIPNVGKSTLINRL-----------AKKNIAKTGDRPGITTSQQWVKVGK--ELELLD 184

Query: 207 TPGIFPPKIHNIEVCSKLALTGAIRDILV 232
           TPGI  PK  +  V  +LA+TGAI+D ++
Sbjct: 185 TPGILWPKFEDELVGLRLAVTGAIKDSII 200

[Close]

BLAST of Lsi08G015290 vs. ExPASy Swiss-Prot
Match: O31743 (Ribosome biogenesis GTPase A OS=Bacillus subtilis (strain 168) OX=224308 GN=rbgA PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 3.8e-26
Identity = 73/209 (34.93%), Postives = 111/209 (53.11%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYEITKNYPPSSKRIIVLNKSDLAD 86
           W+  HMA A R V E++ L+D V E+ DARIPMSS   + ++   +  RI++LNK+D AD
Sbjct: 5   WFPGHMAKARREVTEKLKLIDIVYELVDARIPMSSRNPMIEDILKNKPRIMLLNKADKAD 64

Query: 87  RSQTEVWTRYFEEHNCISYGVNSHNKENIREFL----NFLQARVRELKKSGHSSHATTMM 146
            + T+ W  +FE     S  +NS N + + + +      LQ +   ++  G    A   +
Sbjct: 65  AAVTQQWKEHFENQGIRSLSINSVNGQGLNQIVPASKEILQEKFDRMRAKGVKPRAIRAL 124

Query: 147 LVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGETKNISSLKIASHPNIYVLD 206
           ++GIPNVGKS L N L            K   A    +PG T +   +K+     + +LD
Sbjct: 125 IIGIPNVGKSTLINRL-----------AKKNIAKTGDRPGITTSQQWVKVGK--ELELLD 184

Query: 207 TPGIFPPKIHNIEVCSKLALTGAIRDILV 232
           TPGI  PK  +  V  +LA+TGAI+D ++
Sbjct: 185 TPGILWPKFEDELVGLRLAVTGAIKDSII 200

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BLAST of Lsi08G015290 vs. ExPASy TrEMBL
Match: A0A1S3BA35 (DAR GTPase 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487836 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 2.8e-181
Identity = 331/367 (90.19%), Postives = 344/367 (93.73%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAATLMRKIGTAI   A GNRISSGWYD HMAAASRAVAERIPLVDFVLEVRDARIPMS
Sbjct: 1   MAAATLMRKIGTAINELAAGNRISSGWYDAHMAAASRAVAERIPLVDFVLEVRDARIPMS 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KN+PPSSKRIIVLNK+DLAD+ QTEVWTRYFE+HNCISY VNSHNKENIREFLN
Sbjct: 61  SEYEMMKNHPPSSKRIIVLNKTDLADQLQTEVWTRYFEDHNCISYCVNSHNKENIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQ+RVRELK+SGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHA+ SSQ
Sbjct: 121 FLQSRVRELKRSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSSQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPN+YVLDTPGIFPPKI NIEVCSKLALTGAIRDILVGEHVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNVYVLDTPGIFPPKIDNIEVCSKLALTGAIRDILVGEHVIVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LTIVNSS KYKKWANLSA  +SLECST SSLEKQKRRYPSDHTQDIIVN VRR LFET S
Sbjct: 241 LTIVNSSVKYKKWANLSA--SSLECSTPSSLEKQKRRYPSDHTQDIIVNEVRRSLFETRS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           SF GNLEDEKDMG+LIETQLH LHKALHVPMDFC+  +IKVASKLLNLYRTGRLG YTLD
Sbjct: 301 SFDGNLEDEKDMGSLIETQLHTLHKALHVPMDFCNSTTIKVASKLLNLYRTGRLGRYTLD 360

Query: 361 SLPPVNT 368
           SLP VNT
Sbjct: 361 SLPLVNT 365

[Close]

BLAST of Lsi08G015290 vs. ExPASy TrEMBL
Match: A0A0A0LNT3 (G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G354730 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 9.1e-180
Identity = 328/367 (89.37%), Postives = 344/367 (93.73%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAATLMRKIGTAI   A GNRISSGWYD HM AASRAVAERIPLVDFVLEVRDARIP S
Sbjct: 1   MAAATLMRKIGTAINQLAAGNRISSGWYDAHMEAASRAVAERIPLVDFVLEVRDARIPTS 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KN+PPSSKRIIVLNK+DLAD+SQTEVWTR+FE+HNCISYGVNSHNKENIREFLN
Sbjct: 61  SEYEMMKNHPPSSKRIIVLNKTDLADQSQTEVWTRHFEDHNCISYGVNSHNKENIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHA+ SSQ
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSSQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGIFPPKI +IEVCSKLALTGAIRDILVGEHVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIDDIEVCSKLALTGAIRDILVGEHVIVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LTIVNSS KYKKWANLSA   SLECST S+LEKQ+RRYPSDHTQDI VN VRR LFET S
Sbjct: 241 LTIVNSSVKYKKWANLSAI--SLECSTPSNLEKQRRRYPSDHTQDITVNEVRRALFETRS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           SF GNLEDEK+MG+LIETQLH LHKALHVPMDFC++A+IKVA+KLLNLYRTGRLG YTLD
Sbjct: 301 SFDGNLEDEKEMGSLIETQLHTLHKALHVPMDFCNNATIKVAAKLLNLYRTGRLGRYTLD 360

Query: 361 SLPPVNT 368
           SLP VNT
Sbjct: 361 SLPLVNT 365

[Close]

BLAST of Lsi08G015290 vs. ExPASy TrEMBL
Match: A0A6J1HE44 (DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462723 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.3e-175
Identity = 318/363 (87.60%), Postives = 336/363 (92.56%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAATLMRKIG+AI   A G+RISSGWYDPHMAAASRAV ERIPLVDFVLEVRDARIP +
Sbjct: 1   MAAATLMRKIGSAINEAALGSRISSGWYDPHMAAASRAVVERIPLVDFVLEVRDARIPKT 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KNYP SSKRII+LNK+DLADRSQTE WTR+FE+HNCISYGVNSHNKENIREFL 
Sbjct: 61  SEYELMKNYPLSSKRIIILNKTDLADRSQTEAWTRFFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+AI S Q
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGIFPPKI NIEVCSKLALTGAIRDILVGE ++VQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKILNIEVCSKLALTGAIRDILVGEQLLVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LT++NSSDKYKKW NLSATD+ LECST  SLEKQKR YPSDHTQDIIVN VRRILFETIS
Sbjct: 241 LTLINSSDKYKKWGNLSATDSLLECSTCPSLEKQKRGYPSDHTQDIIVNDVRRILFETIS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           +F GNLEDEK MGNLIETQL ALHKALHVPM F +D+ IKVASKLLNLYRTGRLG YT+D
Sbjct: 301 TFDGNLEDEKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYTID 360

Query: 361 SLP 364
           SLP
Sbjct: 361 SLP 363

[Close]

BLAST of Lsi08G015290 vs. ExPASy TrEMBL
Match: A0A6J1KB62 (DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492297 PE=4 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.3e-173
Identity = 316/363 (87.05%), Postives = 335/363 (92.29%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           M AATLMRKIG+AI   A G+RI+SGWYDPHMAAASRAV ERIPLVDFVLEVRDARIP +
Sbjct: 1   MDAATLMRKIGSAINEAAIGSRITSGWYDPHMAAASRAVVERIPLVDFVLEVRDARIPKT 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KNY PSSKRII+LNK+DLADRSQTE WTR FE+HNCISYGVNSHNKENIREFL 
Sbjct: 61  SEYELMKNYLPSSKRIIILNKTDLADRSQTEAWTRLFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+AI S Q
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGIFPPKI NIEVCSKLALTGAIRDILVGE V+VQ+L
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKILNIEVCSKLALTGAIRDILVGEQVLVQFL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LT++NSSDKYKKW NLSATD+ LECST SSLEK+KR YPSDHTQDIIVN VRRILFETIS
Sbjct: 241 LTLINSSDKYKKWGNLSATDSLLECSTCSSLEKRKRGYPSDHTQDIIVNDVRRILFETIS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           +F GNLED+K MGNLIETQL ALHKALHVPM F +D+ IKVASKLLNLYRTGRLG YTLD
Sbjct: 301 TFDGNLEDKKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYTLD 360

Query: 361 SLP 364
           SLP
Sbjct: 361 SLP 363

[Close]

BLAST of Lsi08G015290 vs. ExPASy TrEMBL
Match: A0A6J1D0W3 (DAR GTPase 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015994 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.4e-172
Identity = 315/365 (86.30%), Postives = 332/365 (90.96%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAAT  R+IGTAIK  A GNR S GWYDPHMAAASRAVAERIPLVDFVLEVRDARIP+S
Sbjct: 1   MAAATFARRIGTAIKETAIGNRTSGGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPVS 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYEI KN+ PSSKRIIV+NK DLADRSQTEVWT+YFE+HNC+SYGVN+HNK+NIREFLN
Sbjct: 61  SEYEIMKNHSPSSKRIIVMNKVDLADRSQTEVWTKYFEDHNCVSYGVNAHNKDNIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHA+ S +
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSPE 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGI PPKI NIEVCSKLALTGAIRDILVGE VIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGERVIVQYL 240

Query: 241 LTIVNSSDKYKKWANLSAT--DNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFET 300
           LTI+NSSDKYKKWA+LSAT  D+SLECST S+ E QKR YPSDHTQD IVN VRRILFET
Sbjct: 241 LTILNSSDKYKKWASLSATNCDSSLECSTCSNSESQKRGYPSDHTQDFIVNDVRRILFET 300

Query: 301 ISSFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYT 360
           ISSF G  EDEKDMGN IE QL ALHKALHVPMD  +DAS KVASKLLNLYRTGRLG YT
Sbjct: 301 ISSFDGKTEDEKDMGNFIEVQLLALHKALHVPMDSSNDASTKVASKLLNLYRTGRLGRYT 360

Query: 361 LDSLP 364
           LDS+P
Sbjct: 361 LDSVP 365

[Close]

BLAST of Lsi08G015290 vs. NCBI nr
Match: XP_038884377.1 (DAR GTPase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 684.9 bits (1766), Expect = 3.9e-193
Identity = 347/367 (94.55%), Postives = 355/367 (96.73%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MA ATLMRKIGTAIK  ATG+RISSGWYDPHMAAASRAVAERIPL DFVLEVRDAR+PMS
Sbjct: 1   MAGATLMRKIGTAIKEAATGSRISSGWYDPHMAAASRAVAERIPLADFVLEVRDARVPMS 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYEI KNYPPSSKRIIVLNK+DLADRSQTEVWTRYFE+HNCISYGVNSHNKENIREFLN
Sbjct: 61  SEYEIMKNYPPSSKRIIVLNKADLADRSQTEVWTRYFEDHNCISYGVNSHNKENIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKH I SSQ
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHTIVSSQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LTIVNSSDKYKKWANLSATD+SLECS +S+LEKQKRRYPSDHTQDIIVNGVRRILFETIS
Sbjct: 241 LTIVNSSDKYKKWANLSATDSSLECS-NSNLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           SF GNLEDEKDMG LIETQLHALHKALHVPMD C+DASIKVASKLLNLYRTGRLGCYTLD
Sbjct: 301 SFDGNLEDEKDMGILIETQLHALHKALHVPMDLCNDASIKVASKLLNLYRTGRLGCYTLD 360

Query: 361 SLPPVNT 368
           SLPPV T
Sbjct: 361 SLPPVKT 366

[Close]

BLAST of Lsi08G015290 vs. NCBI nr
Match: XP_008444550.1 (PREDICTED: DAR GTPase 2, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 644.4 bits (1661), Expect = 5.8e-181
Identity = 331/367 (90.19%), Postives = 344/367 (93.73%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAATLMRKIGTAI   A GNRISSGWYD HMAAASRAVAERIPLVDFVLEVRDARIPMS
Sbjct: 1   MAAATLMRKIGTAINELAAGNRISSGWYDAHMAAASRAVAERIPLVDFVLEVRDARIPMS 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KN+PPSSKRIIVLNK+DLAD+ QTEVWTRYFE+HNCISY VNSHNKENIREFLN
Sbjct: 61  SEYEMMKNHPPSSKRIIVLNKTDLADQLQTEVWTRYFEDHNCISYCVNSHNKENIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQ+RVRELK+SGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHA+ SSQ
Sbjct: 121 FLQSRVRELKRSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSSQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPN+YVLDTPGIFPPKI NIEVCSKLALTGAIRDILVGEHVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNVYVLDTPGIFPPKIDNIEVCSKLALTGAIRDILVGEHVIVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LTIVNSS KYKKWANLSA  +SLECST SSLEKQKRRYPSDHTQDIIVN VRR LFET S
Sbjct: 241 LTIVNSSVKYKKWANLSA--SSLECSTPSSLEKQKRRYPSDHTQDIIVNEVRRSLFETRS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           SF GNLEDEKDMG+LIETQLH LHKALHVPMDFC+  +IKVASKLLNLYRTGRLG YTLD
Sbjct: 301 SFDGNLEDEKDMGSLIETQLHTLHKALHVPMDFCNSTTIKVASKLLNLYRTGRLGRYTLD 360

Query: 361 SLPPVNT 368
           SLP VNT
Sbjct: 361 SLPLVNT 365

[Close]

BLAST of Lsi08G015290 vs. NCBI nr
Match: XP_004143028.1 (DAR GTPase 2, mitochondrial [Cucumis sativus] >KGN62447.1 hypothetical protein Csa_018789 [Cucumis sativus])

HSP 1 Score: 639.4 bits (1648), Expect = 1.9e-179
Identity = 328/367 (89.37%), Postives = 344/367 (93.73%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAATLMRKIGTAI   A GNRISSGWYD HM AASRAVAERIPLVDFVLEVRDARIP S
Sbjct: 1   MAAATLMRKIGTAINQLAAGNRISSGWYDAHMEAASRAVAERIPLVDFVLEVRDARIPTS 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KN+PPSSKRIIVLNK+DLAD+SQTEVWTR+FE+HNCISYGVNSHNKENIREFLN
Sbjct: 61  SEYEMMKNHPPSSKRIIVLNKTDLADQSQTEVWTRHFEDHNCISYGVNSHNKENIREFLN 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHA+ SSQ
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAVVSSQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGIFPPKI +IEVCSKLALTGAIRDILVGEHVIVQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIDDIEVCSKLALTGAIRDILVGEHVIVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LTIVNSS KYKKWANLSA   SLECST S+LEKQ+RRYPSDHTQDI VN VRR LFET S
Sbjct: 241 LTIVNSSVKYKKWANLSAI--SLECSTPSNLEKQRRRYPSDHTQDITVNEVRRALFETRS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           SF GNLEDEK+MG+LIETQLH LHKALHVPMDFC++A+IKVA+KLLNLYRTGRLG YTLD
Sbjct: 301 SFDGNLEDEKEMGSLIETQLHTLHKALHVPMDFCNNATIKVAAKLLNLYRTGRLGRYTLD 360

Query: 361 SLPPVNT 368
           SLP VNT
Sbjct: 361 SLPLVNT 365

[Close]

BLAST of Lsi08G015290 vs. NCBI nr
Match: XP_022962198.1 (DAR GTPase 2, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 4.8e-175
Identity = 318/363 (87.60%), Postives = 336/363 (92.56%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAATLMRKIG+AI   A G+RISSGWYDPHMAAASRAV ERIPLVDFVLEVRDARIP +
Sbjct: 1   MAAATLMRKIGSAINEAALGSRISSGWYDPHMAAASRAVVERIPLVDFVLEVRDARIPKT 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KNYP SSKRII+LNK+DLADRSQTE WTR+FE+HNCISYGVNSHNKENIREFL 
Sbjct: 61  SEYELMKNYPLSSKRIIILNKTDLADRSQTEAWTRFFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+AI S Q
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGIFPPKI NIEVCSKLALTGAIRDILVGE ++VQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKILNIEVCSKLALTGAIRDILVGEQLLVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LT++NSSDKYKKW NLSATD+ LECST  SLEKQKR YPSDHTQDIIVN VRRILFETIS
Sbjct: 241 LTLINSSDKYKKWGNLSATDSLLECSTCPSLEKQKRGYPSDHTQDIIVNDVRRILFETIS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           +F GNLEDEK MGNLIETQL ALHKALHVPM F +D+ IKVASKLLNLYRTGRLG YT+D
Sbjct: 301 TFDGNLEDEKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYTID 360

Query: 361 SLP 364
           SLP
Sbjct: 361 SLP 363

[Close]

BLAST of Lsi08G015290 vs. NCBI nr
Match: XP_023546448.1 (DAR GTPase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624.8 bits (1610), Expect = 4.8e-175
Identity = 318/363 (87.60%), Postives = 336/363 (92.56%), Query Frame = 0


Query: 1   MAAATLMRKIGTAIKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMS 60
           MAAATLMRKIG+AI   A G+RISSGWYDPHMAAASRAV ERIPLVDFVLEVRDAR+P +
Sbjct: 1   MAAATLMRKIGSAINEAAIGSRISSGWYDPHMAAASRAVVERIPLVDFVLEVRDARVPKT 60

Query: 61  SEYEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLN 120
           SEYE+ KNYPPSSKRII+LNK+DLADRSQTE WTR+FE+HNCISYGVNSHNKENIREFL 
Sbjct: 61  SEYELMKNYPPSSKRIIILNKTDLADRSQTEAWTRFFEDHNCISYGVNSHNKENIREFLT 120

Query: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQ 180
           FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLK+AI S Q
Sbjct: 121 FLQARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKNAIVSPQ 180

Query: 181 PGETKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYL 240
           PGETKNISSLKIASHPNIYVLDTPGI PPKI NIEVCSKLALTGAIRDILVGE V+VQYL
Sbjct: 181 PGETKNISSLKIASHPNIYVLDTPGILPPKILNIEVCSKLALTGAIRDILVGEQVVVQYL 240

Query: 241 LTIVNSSDKYKKWANLSATDNSLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 300
           LT++NSSDKYKKW NLSATD+ LE ST SSLEKQKR YPSDHTQDIIVN VRRILFETIS
Sbjct: 241 LTLINSSDKYKKWGNLSATDSLLERSTCSSLEKQKRGYPSDHTQDIIVNDVRRILFETIS 300

Query: 301 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 360
           +F GNLEDEK MGNLIETQL ALHKALHVPM F +D+ IKVASKLLNLYRTGRLG YT+D
Sbjct: 301 TFDGNLEDEKGMGNLIETQLLALHKALHVPMGFDNDSPIKVASKLLNLYRTGRLGRYTID 360

Query: 361 SLP 364
           SLP
Sbjct: 361 SLP 363

[Close]

BLAST of Lsi08G015290 vs. TAIR 10
Match: AT4G10650.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 432.2 bits (1110), Expect = 4.2e-121
Identity = 227/360 (63.06%), Postives = 275/360 (76.39%), Query Frame = 0


Query: 6   LMRKIGTA-IKGEATGNRISSGWYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYE 65
           + R+IG A IK     NR    WY PHMAAA RA++ERIPLVDFVLE+RDARIP+SSEYE
Sbjct: 9   IAREIGDAVIKASRNPNR---RWYGPHMAAAVRAISERIPLVDFVLEIRDARIPLSSEYE 68

Query: 66  ITKNYPP-SSKRIIVLNKSDLADRSQTEVWTRYFEEHNCISYGVNSHNKENIREFLNFLQ 125
           + + + P  SKRIIVLNK +LAD  + +    YFEE N +SY VNSHNK+ +++ LNFLQ
Sbjct: 69  LLRKFSPLPSKRIIVLNKMELADPLELKKCIDYFEERNYLSYAVNSHNKDCVKQLLNFLQ 128

Query: 126 ARVRELKKSGHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGE 185
           ++VREL K+GHS H TTMML+GIPNVGKSAL+NSLH IGRISAAEKGKLKH   SSQPG+
Sbjct: 129 SQVRELHKAGHSGHTTTMMLLGIPNVGKSALSNSLHHIGRISAAEKGKLKHTTVSSQPGD 188

Query: 186 TKNISSLKIASHPNIYVLDTPGIFPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYLLTI 245
           TK+I SLKI SHPN+YVLDTPGIFPP +++ E+C+KLALTGAI D +VGE  + +  LTI
Sbjct: 189 TKDIMSLKIGSHPNVYVLDTPGIFPPNLYDAEICAKLALTGAIPDDIVGELKLARLFLTI 248

Query: 246 VNSSDKYKKWANLSATDN---SLECSTSSSLEKQKRRYPSDHTQDIIVNGVRRILFETIS 305
           +NSS +YKKWA L  + +   SL   +S S  K KR+Y +DHTQD IV  VRR+L+ETIS
Sbjct: 249 LNSSHEYKKWAKLCKSQDLTESLSDESSKSDAKHKRQYATDHTQDFIVYDVRRVLYETIS 308

Query: 306 SFVGNLEDEKDMGNLIETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLD 361
           +F GNLEDE  MGNLIETQ  AL   L VP +    A ++VASK+LNLYRTGRLG YTL+
Sbjct: 309 AFDGNLEDEISMGNLIETQFAALRSVLRVPEEASEFADLRVASKILNLYRTGRLGHYTLE 365

[Close]

BLAST of Lsi08G015290 vs. TAIR 10
Match: AT2G41670.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 6.9e-63
Identity = 139/352 (39.49%), Postives = 203/352 (57.67%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYEITKNYPPSSKRIIVLNKSDLAD 86
           W+  HMAAA+RA+  R+ L D V+EVRDARIP+SS  E  ++   + +RII LNK DLA+
Sbjct: 28  WFPGHMAAATRAIRNRLKLSDLVIEVRDARIPLSSANEDLQSQMSAKRRIIALNKKDLAN 87

Query: 87  RSQTEVWTRYFEEHNCISYGVNSHNKENIREFLNFLQARVRELKKSGHSSHATTMMLVGI 146
            +    WTR+FE        +N+H++ ++ + L+ ++ +++E+           +M+VG+
Sbjct: 88  PNVLNKWTRHFESSKQDCIAINAHSRSSVMKLLDLVELKLKEVIA---REPTLLVMVVGV 147

Query: 147 PNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGETKNISSLKIASHPNIYVLDTPGI 206
           PNVGKSAL NS+HQI       + +LK A     PG T++I+  KIA  P+IYVLD+PG+
Sbjct: 148 PNVGKSALINSIHQIAAARFPVQERLKRATVGPLPGVTQDIAGFKIAHRPSIYVLDSPGV 207

Query: 207 FPPKIHNIEVCSKLALTGAIRDILVGEHVIVQYLLTIVNSSDKYKKWANLSATDNS---L 266
             P I +IE   KLAL+G+++D +VGE  I QY L I+N       W  L    N     
Sbjct: 208 LVPSIPDIETGLKLALSGSVKDSVVGEERIAQYFLAILNIRGTPLHWKYLVEGINEGPHA 267

Query: 267 ECSTSSS-----LEKQKRRYPSD---HTQDIIVNGVRRILFETISSFVGNLEDEKDMGNL 326
           +C    S     L  Q+ + P     H    +++ V+R L+ T+S F G+ EDE D+  L
Sbjct: 268 DCIDKPSYNLKDLRHQRTKQPDSSALHYVGDMISEVQRSLYITLSEFDGDTEDENDLECL 327

Query: 327 IETQLHALHKALHVPMDFCSDASIKVASKLLNLYRTGRLGCYTLDSLPPVNT 368
           IE Q   L KAL +P    S+A + V+ K L L+RTGRLG + LD +P   T
Sbjct: 328 IEQQFEVLQKALKIPHK-ASEARLMVSKKFLTLFRTGRLGPFILDDVPETET 375

[Close]

BLAST of Lsi08G015290 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.7e-16
Identity = 57/200 (28.50%), Postives = 96/200 (48.00%), Query Frame = 0


Query: 27  WYDPHMAAASRAVAERIPLVDFVLEVRDARIPMSSEYEITKNYPPSSKRIIVLNKSDLAD 86
           WY  H+    + + E++ L+D V+EVRDARIP+S+ +     +  + KRI+VLN+ D+  
Sbjct: 98  WYPGHIMKTEKELREQLKLMDVVIEVRDARIPLSTTHPKMDAWLGNRKRILVLNREDMIS 157

Query: 87  RSQTEVWTRYFEEHN---CISYGVNSHNKENIREFLNFLQARVR-ELKKSGHSSHATTMM 146
                 W RYF +       + G        +      L   V  + ++ G    +    
Sbjct: 158 NDDRNDWARYFAKQGIKVIFTNGKLGMGAMKLGRLAKSLAGDVNGKRREKGLLPRSVRAG 217

Query: 147 LVGIPNVGKSALANSLHQIGRISAAEKGKLKHAIASSQPGETKNISSLKIASHPNIYVLD 206
           ++G PNVGKS+L N L            K K   A+ +PG T+ +  +K+    ++ +LD
Sbjct: 218 IIGYPNVGKSSLINRLL-----------KRKICAAAPRPGVTREMKWVKLGK--DLDLLD 277

Query: 207 TPGIFPPKIHNIEVCSKLAL 223
           +PG+ P +I +     KLA+
Sbjct: 278 SPGMLPMRIDDQAAAIKLAI 284

[Close]

BLAST of Lsi08G015290 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 55.8 bits (133), Expect = 8.3e-08
Identity = 53/195 (27.18%), Postives = 85/195 (43.59%), Query Frame = 0


Query: 43  IPLVDFVLEVRDARIPMSSE----YEITKNYPPSSKRIIVLNKSDLADRSQTEVWTRYF- 102
           I L D +LEV DAR P+ +       +     P+   +++LNK DL  R   E W  Y  
Sbjct: 134 IELSDVILEVLDARDPLGTRCTDMERMVMQAGPNKHLVLLLNKIDLVPREAAEKWLMYLR 193

Query: 103 EEHNCISYGVNSHNKEN--------IREFLNFLQ-----------------ARVRELKKS 162
           EE   +++  ++  + +          +  N LQ                 +R  ELKKS
Sbjct: 194 EEFPAVAFKCSTQEQRSNLGWKSSKASKPSNMLQTSDCLGADTLIKLLKNYSRSHELKKS 253

Query: 163 GHSSHATTMMLVGIPNVGKSALANSLHQIGRISAAEKGKLKHAI-ASSQPGETKNISSLK 207
                  T+ ++G+PNVGKS+L NSL            K  H +   + PG T+++  + 
Sbjct: 254 ------ITVGIIGLPNVGKSSLINSL------------KRAHVVNVGATPGLTRSLQEVH 308

The following BLAST results are available for this feature:

BLAST of Lsi08G015290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82497	6.0e-120	63.06	DAR GTPase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DGP2 PE=3 SV=1	[more]
Q8L607	9.7e-62	39.49	Short integuments 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SIN2 PE=1 ...	[more]
Q65JP4	1.3e-26	30.88	Ribosome biogenesis GTPase A OS=Bacillus licheniformis (strain ATCC 14580 / DSM ...	[more]
E0TTS5	3.8e-26	34.93	Ribosome biogenesis GTPase A OS=Bacillus subtilis subsp. spizizenii (strain ATCC...	[more]
O31743	3.8e-26	34.93	Ribosome biogenesis GTPase A OS=Bacillus subtilis (strain 168) OX=224308 GN=rbgA...	[more]

BLAST of Lsi08G015290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BA35	2.8e-181	90.19	DAR GTPase 2, mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487836 P...	[more]
A0A0A0LNT3	9.1e-180	89.37	G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G354730 PE=4 SV=1	[more]
A0A6J1HE44	2.3e-175	87.60	DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1KB62	1.3e-173	87.05	DAR GTPase 2, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114922...	[more]
A0A6J1D0W3	2.4e-172	86.30	DAR GTPase 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Lsi08G015290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884377.1	3.9e-193	94.55	DAR GTPase 2, mitochondrial [Benincasa hispida]	[more]
XP_008444550.1	5.8e-181	90.19	PREDICTED: DAR GTPase 2, mitochondrial isoform X1 [Cucumis melo]	[more]
XP_004143028.1	1.9e-179	89.37	DAR GTPase 2, mitochondrial [Cucumis sativus] >KGN62447.1 hypothetical protein C...	[more]
XP_022962198.1	4.8e-175	87.60	DAR GTPase 2, mitochondrial isoform X1 [Cucurbita moschata]	[more]
XP_023546448.1	4.8e-175	87.60	DAR GTPase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi08G015290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10650.1	4.2e-121	63.06	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G41670.1	6.9e-63	39.49	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT4G02790.1	1.7e-16	28.50	GTP-binding family protein	[more]
AT3G07050.1	8.3e-08	27.18	GTP-binding family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 112..132
None	No IPR available	PIRSR	PIRSR006230-1	PIRSR006230-1	coord: 27..299 e-value: 2.6E-49 score: 165.8
None	No IPR available	PANTHER	PTHR45782:SF1	DAR GTPASE 2, MITOCHONDRIAL	coord: 3..366
None	No IPR available	PANTHER	PTHR45782	MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1	coord: 3..366
None	No IPR available	CDD	cd01856	YlqF	coord: 27..207 e-value: 1.10758E-57 score: 183.499
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 25..207 e-value: 7.8E-34 score: 118.8
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 31..220
IPR006073	GTP binding domain	PFAM	PF01926	MMR_HSR1	coord: 141..251 e-value: 4.9E-12 score: 45.9
IPR030378	Circularly permuted (CP)-type guanine nucleotide-binding (G) domain	PROSITE	PS51721	G_CP	coord: 32..209 score: 34.725128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G015290.1	Lsi08G015290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005743	mitochondrial inner membrane
molecular_function	GO:0003924	GTPase activity
molecular_function	GO:0005525	GTP binding