Lsi08G015260 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G015260 vs. ExPASy Swiss-Prot
Match: O82777 (Subtilisin-like protease SBT3 OS=Solanum lycopersicum OX=4081 GN=sbt3 PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 2.3e-155
Identity = 320/756 (42.33%), Postives = 448/756 (59.26%), Query Frame = 0

Query: 30  LTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSSSFPSKLIHIY 89
           L +   YIVH++ + MP  F   H W+S+TI S+       +  SS        KL++ Y
Sbjct: 21  LAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSI-----KASVPSSVDRFHSAPKLVYSY 80

Query: 90  NHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLNSNFGLLPISNYG 149
           ++ + GFSA  +  EL ALK  PG++S+  D +V   TTH+S FL LN + GL P S  G
Sbjct: 81  DNVLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPASGLG 140

Query: 150 SDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKLIGARFFNKGL 209
            DVI+  +D+GIWPES SF DDGM EIP RWKG C+ GT FN S+CN+KLIGA +FNKG+
Sbjct: 141 QDVIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYFNKGI 200

Query: 210 IAKFP--NLTISAI---------------------------------------------- 269
           +A  P  N+T+++                                               
Sbjct: 201 LANDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLAVYKF 260

Query: 270 -WEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATSA 329
            + E     D+IAA+DQA++DGVD+IS+S G   +PLYED ++IA+F A+ +G+ V+ SA
Sbjct: 261 SFNEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLVSASA 320

Query: 330 GNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSILGSSLFPLNSTMDVSPLPI 389
           GN GP +G+++NG+PW+L VA+G  DR F GT+TL NG+ I G SLFP  +   V   P+
Sbjct: 321 GNRGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAF--VRDSPV 380

Query: 390 VF---MGGCHN---LKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSAKVALGVFISNISDW 449
           ++   +  C +   L +++     IV+C+D   +  S Q+  I  A++   +FIS     
Sbjct: 381 IYNKTLSDCSSEELLSQVENPENTIVICDDNGDF--SDQMRIITRARLKAAIFISEDPGV 440

Query: 450 DNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKPAPSVARYSSRGPSM 509
                   P + ++   G  + +Y+K    P A + F +T L TKPAP VA  S+RGPS 
Sbjct: 441 FRSATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSARGPSR 500

Query: 510 SCPFVLKPDIMAPGDAILASWPHNVAAMDVNST-QIYTKFNVMSGTSMSCPHAAAVAALL 569
           S   + KPDI+APG  ILA++P NV A  + +   + T + + SGTSM+ PHAA +AA+L
Sbjct: 501 SYLGISKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGIAAML 560

Query: 570 KGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSGHVNPNKAIDPDL 629
           K  H  WSP+AIRSAMMTTA  +DNT+  IKD  N NK ATPL MG+GHV+PN+A+DP L
Sbjct: 561 KAAHPEWSPSAIRSAMMTTADPLDNTRKPIKDSDN-NKAATPLDMGAGHVDPNRALDPGL 620

Query: 630 IYDMGIQDYVNLLCALNYTKNQIQTITR-SASNDCKNPSLDLNYPSFIIIVNSSDSKTRK 689
           +YD   QDYVNLLC+LN+T+ Q +TI R SAS++C NPS DLNYPSFI + +   + T  
Sbjct: 621 VYDATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGNFTLL 680

Query: 690 REILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLSYKLKIAGSVR 729
            +   +FKRT+T +G+  ATY+AKL+  K   + V P+ L FK KN+K SY L I     
Sbjct: 681 EQ---KFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIRYIGD 740

BLAST of Lsi08G015260 vs. ExPASy Swiss-Prot
Match: Q9FHA4 (Subtilisin-like protease SBT1.9 OS=Arabidopsis thaliana OX=3702 GN=SBT1.9 PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 5.3e-144
Identity = 316/768 (41.15%), Postives = 430/768 (55.99%), Query Frame = 0

Query: 20  LIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSS 79
           L+  F +     ET  YI+HM+ +A P PF+   SW+S T++SV+              +
Sbjct: 9   LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI--------------T 68

Query: 80  SFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLNSN 139
           +   K+I+ Y  ++ GFSA  T SEL+ LK+ PGYVS   D  V++ TT S  F+GLNS 
Sbjct: 69  NRKPKIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNST 128

Query: 140 FGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKL 199
            G  P+SNYG+ ++IG +DTGIWP+S SF+DDG+  +PS+WKG CE     ++SLCNKKL
Sbjct: 129 SGTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKL 188

Query: 200 IGARFFNKGLIAKFPNL------------------------------------------- 259
           IGA+ FNKGL A  P+L                                           
Sbjct: 189 IGAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTA 248

Query: 260 ----------TISAIWEESNSVLDVIAAIDQAISDGVDVISLSLGL--------DGVPLY 319
                        A WEE     DVIAAIDQAI DGV VISLSLGL        DG  L 
Sbjct: 249 SGIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLE 308

Query: 320 EDPVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNG 379
            DP+A+A+FAA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+T  N 
Sbjct: 309 NDPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNR 368

Query: 380 VSILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQ 439
           VS    SLFP        P+  +  G   N    K +  +IVVC   +  ++ S++  I+
Sbjct: 369 VSFSFPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIR 428

Query: 440 SAKVALGVFISN-ISDWDNLIQTSFPSIFLSPHHGNIIKDYL-KECSDPKAEVNFHKTVL 499
           S   A  V I++ + +  + I+  FP  F+   H   I+ Y     ++  A++ F KTV+
Sbjct: 429 STGAAAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVI 488

Query: 500 GTKPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVM 559
           GTKPAP V  YSSRGP  S P +LKPDI+APG  IL++WP         +  +++ FN++
Sbjct: 489 GTKPAPEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLL 548

Query: 560 SGTSMSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPL 619
           +GTSM+ PH A VAAL+K VH  WSP+AI+SA+MTTA  +DN                PL
Sbjct: 549 TGTSMAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PL 608

Query: 620 AMGSGHVNPNKAIDPDLIYDMGIQDYVNLLC-ALNYTKNQIQTITRSASND-CKNPSLDL 679
           A+G+GHV+ NK ++P LIYD   QD++N LC     ++  I  ITRS  +D CK PS  L
Sbjct: 609 AVGAGHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYL 668

Query: 680 NYPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEF 723
           NYPS I    S  S  +       FKRTLT +GE K +Y  ++RG+KGL V V+PKKL F
Sbjct: 669 NYPSIIAYFTSDQSSPK------IFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMF 728

BLAST of Lsi08G015260 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 8.8e-123
Identity = 276/774 (35.66%), Postives = 399/774 (51.55%), Query Frame = 0

Query: 23  IFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSSSFP 82
           +F L  T T    YI+ +N +  P+ F T H WY++ ++S                    
Sbjct: 18  LFLLLHT-TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------------E 77

Query: 83  SKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVL-DSSVRVDTTHSSHFLGLNSNFG 142
           S L++ Y  +  GFSA    +E ++L +S   +  +  D    + TT +  FLGLNS FG
Sbjct: 78  SSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 137

Query: 143 LLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKLIG 202
           +  + +  + VIIG +DTG+WPES SF+D  M EIPS+WKGECESG+ F++ LCNKKLIG
Sbjct: 138 VHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIG 197

Query: 203 ARFFNK----------------------------------------------------GL 262
           AR F+K                                                    G+
Sbjct: 198 ARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 257

Query: 263 IAKFPNLTISAIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAA 322
             +    T    W       D++AA+D+AI DGVDV+SLSLG    P Y D +AI  F+A
Sbjct: 258 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSA 317

Query: 323 VERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSILGSSLFPL 382
           +ERG+FV+ SAGN+GP   +V N APWV+ V AGT+DRDF     L NG  + G SL+  
Sbjct: 318 MERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS- 377

Query: 383 NSTMDVSPLPIVFMGGCHNLKKLKRIGY--------KIVVCEDKDGYSLSSQVDNIQSAK 442
              M   PL +V+  G  +   L   G         KIVVC+   G +   +   +    
Sbjct: 378 GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCD--RGVNARVEKGAVVRDA 437

Query: 443 VALGVFISN-ISDWDNLIQTS--FPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGT 502
             LG+ ++N  +  + L+  S   P+I +    G+++++Y+K  S P A + F  TVL  
Sbjct: 438 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDV 497

Query: 503 KPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSG 562
           KP+P VA +SSRGP+   P +LKPD++ PG  ILA W   +    ++     T+FN+MSG
Sbjct: 498 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 557

Query: 563 TSMSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAM 622
           TSMSCPH + +A LLK  H  WSP+AI+SA+MTTA V+DNT   + D  + N  + P A 
Sbjct: 558 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAH 617

Query: 623 GSGHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLD---LN 682
           GSGHV+P KA+ P L+YD+  ++Y+  LC+L+YT + I  I +  S +C     D   LN
Sbjct: 618 GSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLN 677

Query: 683 YPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFK 730
           YPSF ++         KR +   + R +T +G   + Y+  + G   + + VKP KL FK
Sbjct: 678 YPSFSVLFGG------KRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFK 737

BLAST of Lsi08G015260 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.4e-117
Identity = 279/767 (36.38%), Postives = 401/767 (52.28%), Query Frame = 0

Query: 36  YIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSSSFPSKLIHIYNHAISG 95
           YIVH++  A P  F T   WY+++++S+               +S P  +IH Y+    G
Sbjct: 28  YIVHVDHEAKPSIFPTHFHWYTSSLASL---------------TSSPPSIIHTYDTVFHG 87

Query: 96  FSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLNS--NFGLLPISNYGSDVI 155
           FSA  T  +   L + P  +S + +    + TT S  FLGL S    GLL  S++GSD++
Sbjct: 88  FSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLV 147

Query: 156 IGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKLIGARFFNKGLIAK- 215
           IG +DTG+WPE  SF+D G+  +P +WKG+C +   F  S CN+KL+GARFF  G  A  
Sbjct: 148 IGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN 207

Query: 216 -------------------------------FPNLTI-------------------SAIW 275
                                          FP  T+                      W
Sbjct: 208 GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW 267

Query: 276 EESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATSAGN 335
                  D++AA D A++DGVDVISLS+G   VP Y D +AI  F A++RGIFV+ SAGN
Sbjct: 268 NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGN 327

Query: 336 NGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSILGSSLFPLNSTMDVSPLPIVF 395
            GP   TV N APW+  V AGT+DRDF   + L NG  I G S++           P+V+
Sbjct: 328 GGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVY 387

Query: 396 ----MGGCHNLKKL--------KRIGYKIVVCEDKDGYSLSSQVDNIQSAKVALGVFISN 455
               +GG      L          +  KIV+C+   G +  +    I      LG+ I+N
Sbjct: 388 GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCD--RGINSRATKGEIVRKNGGLGMIIAN 447

Query: 456 -ISDWDNLIQTS--FPSIFLSPHHGNIIKDYLKECS------DPKAEVNFHKTVLGTKPA 515
            + D + L+      P+  +    G+ I+ Y+ E S       P A + F  T LG +PA
Sbjct: 448 GVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPA 507

Query: 516 PSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSM 575
           P VA +S+RGP+   P +LKPD++APG  ILA+WP  +    V S    T+FN++SGTSM
Sbjct: 508 PVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSM 567

Query: 576 SCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSG 635
           +CPH + +AALLK  H  WSPAAIRSA++TTA  VDN+   + D    N  ++ +  GSG
Sbjct: 568 ACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSG 627

Query: 636 HVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSL-----DLNY 695
           HV+P KA+DP L+YD+   DY+N LC  NYT+  I TITR  + DC          +LNY
Sbjct: 628 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNY 687

Query: 696 PSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKR 722
           PSF ++      +  + ++   F RT+T +G+  + YE K+R  +G  V V+P+KL F+R
Sbjct: 688 PSFSVVF----QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 747

BLAST of Lsi08G015260 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 5.5e-117
Identity = 274/761 (36.01%), Postives = 401/761 (52.69%), Query Frame = 0

Query: 36  YIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSSSFPSKLIHIYNHAISG 95
           +I  ++  +MP  F T + WYS   +                     S+++H+Y+    G
Sbjct: 28  FIFRIDGGSMPSIFPTHYHWYSTEFAE-------------------ESRIVHVYHTVFHG 87

Query: 96  FSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLNSNFGLLPISNYGSDVIIG 155
           FSA  TP E + L+N P  ++   D    + TT S  FLGL +  GL   S+YGSDVIIG
Sbjct: 88  FSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIG 147

Query: 156 FVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKLIGARFFNKGL------ 215
             DTGIWPE  SF+D  +  IP RW+G CESG  F+   CN+K+IGARFF KG       
Sbjct: 148 VFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIG 207

Query: 216 -----------------------------------------IAK--FPNLTISA---IWE 275
                                                    +AK   P   I+A    W+
Sbjct: 208 GINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWK 267

Query: 276 ESNSV-LDVIAAIDQAISDGVDVISLSL-GLDGV--PLYEDPVAIATFAAVERGIFVATS 335
           +S  +  D++AA D A+ DGVDVIS+S+ G DG+  P Y DP+AI ++ A  +GIFV++S
Sbjct: 268 DSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSS 327

Query: 336 AGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSILGSSLF---PLNSTMDVS 395
           AGN GP   +V N APWV  V A T+DR+F     L +G  + G SL+   PLN  M   
Sbjct: 328 AGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM--- 387

Query: 396 PLPIVFMGG---------CHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSAKVALGVF 455
             P+V+ G            N    K++  KIV+C+      ++  +   ++  V + + 
Sbjct: 388 -FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGM-IL 447

Query: 456 ISNISDWDNLIQTS--FPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKPAPSVA 515
            +  S+ + L+  +   P+  +  + G+ IK Y     +P A ++F  T++G KPAP +A
Sbjct: 448 ANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIA 507

Query: 516 RYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSMSCPH 575
            +S RGP+   P +LKPD++APG  ILA+W   V    + S    T+FN++SGTSM+CPH
Sbjct: 508 SFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPH 567

Query: 576 AAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSGHVNP 635
            +  AALLK  H  WSPA IRSAMMTT  +VDN+  S+ D  +  K ATP   GSGH+N 
Sbjct: 568 VSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGHLNL 627

Query: 636 NKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASN--DCKNPSL-DLNYPSFIII 695
            +A++P L+YD+   DY+  LC++ Y    IQ ITR+       + PS  +LNYPS   +
Sbjct: 628 GRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAV 687

Query: 696 VNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 722
             ++      + ++    RT T +G+ +A Y A++   +G+ V VKP +L F    ++ S
Sbjct: 688 FPTNRRGLVSKTVI----RTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRS 747

BLAST of Lsi08G015260 vs. ExPASy TrEMBL
Match: A0A5A7V589 (Subtilisin-like protease SBT1.9 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00840 PE=3 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 614/765 (80.26%), Postives = 662/765 (86.54%), Query Frame = 0

Query: 10  MAARLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLL 69
           MA RLYFWFSL PIF LCP LTET  YIVHMNSAAMPKPFA+RHSWYSATISSVLH+S  
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 70  DTTISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTH 129
            ++ SSSSSSSFPSKLIH YNHAISGF AS TPS+LEALKNSPGY+SSVLDSSV VDTTH
Sbjct: 61  SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 130 SSHFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTH 189
           SSHFLGL+SN GLLPIS YGSDVIIGFVDTGIWP+SESFNDDGMSEIPSRWKGECES TH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180

Query: 190 FNTSLCNKKLIGARFFNKGLIAKFPNLTIS------------------------------ 249
           FN S CN KLIGARFFNKGLIA+FPN TIS                              
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240

Query: 250 -------------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYED 309
                              AIW+E NSV DV+AAIDQAISDGVDVISLS+G+DG+PLY D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300

Query: 310 PVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 369
           PV+IATFAAVERGIFVATSAGNNGP+LGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360

Query: 370 ILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSA 429
           +LGSSLFPLN+TM +SPLPIVFMGGC NLKKLKRIGYKIVVCED DGYSL+SQVDN+Q+A
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420

Query: 430 KVALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKP 489
           KV LG+FISNI DWDNLIQT FPSIF++P+HGNIIKDY+ + SDPKAEVNFHKT+LGTKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480

Query: 490 APSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTS 549
           AP+VARYSSRGPS SCPFVLKPDIMAPGDAILASWP N+ AMDVNST IYTKFNV+SGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540

Query: 550 MSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGS 609
           MSCPHAA VAALLKG H +WSPAAIRSAMMTTA VVDNTQTSIKDIGN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600

Query: 610 GHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFI 669
           GHVNPNKA+DPDLIYD+GIQDYVN+LCALNYT+NQI+ ITRS SN+C+NPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 670 IIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQK 726
           II NSSDSKT KR+ILGEFKRTLTKIGE +ATYEAKLRGMKG KVRVKP+ LEFKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720

BLAST of Lsi08G015260 vs. ExPASy TrEMBL
Match: A0A1S3BBC3 (subtilisin-like protease SBT1.9 OS=Cucumis melo OX=3656 GN=LOC103487840 PE=3 SV=1)

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 614/765 (80.26%), Postives = 662/765 (86.54%), Query Frame = 0

Query: 10  MAARLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLL 69
           MA RLYFWFSL PIF LCP LTET  YIVHMNSAAMPKPFA+RHSWYSATISSVLH+S  
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 70  DTTISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTH 129
            ++ SSSSSSSFPSKLIH YNHAISGF AS TPS+LEALKNSPGY+SSVLDSSV VDTTH
Sbjct: 61  SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 130 SSHFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTH 189
           SSHFLGL+SN GLLPIS YGSDVIIGFVDTGIWP+SESFNDDGMSEIPSRWKGECES TH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180

Query: 190 FNTSLCNKKLIGARFFNKGLIAKFPNLTIS------------------------------ 249
           FN S CN KLIGARFFNKGLIA+FPN TIS                              
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240

Query: 250 -------------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYED 309
                              AIW+E NSV DV+AAIDQAISDGVDVISLS+G+DG+PLY D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300

Query: 310 PVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 369
           PV+IATFAAVERGIFVATSAGNNGP+LGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360

Query: 370 ILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSA 429
           +LGSSLFPLN+TM +SPLPIVFMGGC NLKKLKRIGYKIVVCED DGYSL+SQVDN+Q+A
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420

Query: 430 KVALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKP 489
           KV LG+FISNI DWDNLIQT FPSIF++P+HGNIIKDY+ + SDPKAEVNFHKT+LGTKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480

Query: 490 APSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTS 549
           AP+VARYSSRGPS SCPFVLKPDIMAPGDAILASWP N+ AMDVNST IYTKFNV+SGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540

Query: 550 MSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGS 609
           MSCPHAA VAALLKG H +WSPAAIRSAMMTTA VVDNTQTSIKDIGN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600

Query: 610 GHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFI 669
           GHVNPNKA+DPDLIYD+GIQDYVN+LCALNYT+NQI+ ITRS SN+C+NPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 670 IIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQK 726
           II NSSDSKT KR+ILGEFKRTLTKIGE +ATYEAKLRGMKG KVRVKP+ LEFKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720

BLAST of Lsi08G015260 vs. ExPASy TrEMBL
Match: A0A0A0LKL6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G354760 PE=3 SV=1)

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 600/765 (78.43%), Postives = 649/765 (84.84%), Query Frame = 0

Query: 10  MAARLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLL 69
           MAARLYFWFSLIPIF LCP LTET  YIVHMNSAAMPKPFA+RHSWYSATISS+LH+   
Sbjct: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHS--- 60

Query: 70  DTTISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTH 129
               SSSSSSSFPSKLIH YNHAISGF AS TPS+LEALKNSPGY+SSVLDSSV VDTTH
Sbjct: 61  ----SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 130 SSHFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTH 189
           SSHFLGL+SN GLLPIS YGSDVIIGFVDTGIWP+SESF DDGMSEIPS+WKGECES TH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTH 180

Query: 190 FNTSLCNKKLIGARFFNKGLIAKFPNLTIS------------------------------ 249
           FN S CN KLIGARFFNKGLI+  P  TIS                              
Sbjct: 181 FNVSFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFG 240

Query: 250 -------------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYED 309
                              AIWEE NSV DV+AAIDQAISDGVDVISLS+G+DGVPLY+D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDD 300

Query: 310 PVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 369
           PVAIATFAAVERGIFVATSAGNNGP+L TVHNGAPW+LNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVAIATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVS 360

Query: 370 ILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSA 429
           +LGSSLFPLN T  +SPLPIVFMGGC NLKKL+R GYKIVVCED DGYSL+SQVDN+Q+A
Sbjct: 361 VLGSSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTA 420

Query: 430 KVALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKP 489
            VALG+FISNI DWDNLIQT FPSIFL+P+HGNIIKDY+ + SDPKAEV FHKT+L TKP
Sbjct: 421 NVALGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKP 480

Query: 490 APSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTS 549
           AP VARYSSRGPS SCPFVLKPDIMAPGD ILASWP NV AMDVNST IY+KFNV+SGTS
Sbjct: 481 APMVARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTS 540

Query: 550 MSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGS 609
           MSCPHAA VAALLKG H +WSPAAIRSAMMTTA ++DNTQT IKD GN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGS 600

Query: 610 GHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFI 669
           GHVNPNKAIDPDLIYD+GIQDYVN+LCALNYT+NQI+ ITRS SN+C+NPSLDLNYPSFI
Sbjct: 601 GHVNPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 670 IIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQK 726
           +IVNSSDSKTRKR+I GEFKRTLTKIGE +ATYEAKL GMKG KVRVKP KL FKRKNQK
Sbjct: 661 MIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQK 720

BLAST of Lsi08G015260 vs. ExPASy TrEMBL
Match: A0A6J1HH07 (subtilisin-like protease SBT1.9 OS=Cucurbita moschata OX=3662 GN=LOC111462861 PE=3 SV=1)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 576/770 (74.81%), Postives = 638/770 (82.86%), Query Frame = 0

Query: 13  RLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSV-LHTSLLDT 72
           R++FW SL+ IF    TLT+TD YIVHM+ AAMPKPFAT HSWYSAT+SSV L TS L T
Sbjct: 6   RMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 73  TISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSS 132
           T +SSSSSS PSKLIH Y HAI+GF+AS TPS+L+ALKNSPGYVSS+ DSSVR DTTHSS
Sbjct: 66  T-TSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSS 125

Query: 133 HFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFN 192
           +FL L+ N GLLPISNYGSDVIIGFVDTG+WPESESFNDDG+S+IPSRWKGECESGTHFN
Sbjct: 126 NFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFN 185

Query: 193 TSLCNKKLIGARFFNKGLIAKFPNLTIS-------------------------------- 252
            SLCNKKLIG RFFNKGLI+KFPN+TIS                                
Sbjct: 186 ASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG 245

Query: 253 -----------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 312
                            AIW+E N+V DVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV
Sbjct: 246 QGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 305

Query: 313 AIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSIL 372
           AIATFAA+ERGIFVATSAGN GP+ GTVH+GAPWVLNVAAGTMDRDFGGTITL+NGVS+L
Sbjct: 306 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVL 365

Query: 373 GSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSAKV 432
           GSSLFPLNS + +SPLPIVFMG CHNLKKLKR+G+KIVVCED D YSL  QVDN++SAK+
Sbjct: 366 GSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKI 425

Query: 433 ALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKPAP 492
           A+GVFISNISDWDNLIQTSFPSIFL+ +HGN+IKDY+K  S+PKA VNFHKT++GTKPAP
Sbjct: 426 AVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAP 485

Query: 493 SVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSMS 552
           SVARYSSRGPS SCPFVLKPDIMAPGDAILASWP NVAA DV S  IY+KFNV+SGTSM+
Sbjct: 486 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMA 545

Query: 553 CPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSGH 612
           CPHAA VAALLKG H  WSPAAIRSAMMTTA +VDNTQT IKD+GNKNK ATPLAMGSGH
Sbjct: 546 CPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGH 605

Query: 613 VNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFIII 672
           VNPNKAIDP LIYDMGI+DY NLLCALNYTKNQIQTITRS SN C+   LDLNYPSFI+ 
Sbjct: 606 VNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMT 665

Query: 673 VNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 730
           VN+SDS+T + E+  EFKR +T IGEK ATY AK+  MKGL V V+P KL+FKRKNQ LS
Sbjct: 666 VNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLS 725

BLAST of Lsi08G015260 vs. ExPASy TrEMBL
Match: A0A6J1K9W2 (subtilisin-like protease SBT1.9 OS=Cucurbita maxima OX=3661 GN=LOC111491986 PE=3 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 576/767 (75.10%), Postives = 632/767 (82.40%), Query Frame = 0

Query: 13  RLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSV-LHTSLLDT 72
           R++F  SL+ I     TLTETD YIVHM+ AAMPKPFAT HSWYSAT+SSV L TS L T
Sbjct: 6   RMFFCLSLMVILLARSTLTETDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 73  TISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSS 132
           T  +SSSSS PSKLIH Y HAI+GF+A+ TPS+L+ALKNSPGYVSSV DSSVR DTTHSS
Sbjct: 66  T--TSSSSSLPSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSS 125

Query: 133 HFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFN 192
           +FL L  N GLLPISNYGSDVIIGFVDTG+WPESESFND+ +S+IPSRWKGECESGTHFN
Sbjct: 126 NFLALTPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDEAISKIPSRWKGECESGTHFN 185

Query: 193 TSLCNKKLIGARFFNKGLIAKFPNLTIS-------------------------------- 252
            SLCNKKLIG RFFNKGLIAKFPN+TIS                                
Sbjct: 186 ASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG 245

Query: 253 -----------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 312
                            AIW+E N V DVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV
Sbjct: 246 QGTARGVAPRARVAIYKAIWKEGNVVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 305

Query: 313 AIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSIL 372
           AIATFAA+ERGIFVATSAGN GP+ GTVH+GAPWVLNVAAGTMDRDFGGTITL+NGVS+L
Sbjct: 306 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLNNGVSVL 365

Query: 373 GSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSAKV 432
           GSSLF LNS + +SPLPIVFMG CHNLKKLKR+G+KIVVCED D YSL  QVDN+QSAK+
Sbjct: 366 GSSLFVLNSAIVLSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVQSAKI 425

Query: 433 ALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKPAP 492
           A+GVFISNISDWDNLIQTSFPSIFL+ +HGN+IKDY+   S+PKA VNFHKT++GTKPAP
Sbjct: 426 AVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIMRSSNPKARVNFHKTIIGTKPAP 485

Query: 493 SVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSMS 552
           SVARYSSRGPS SCP VLKPDIMAPGDAILASWP  VAA DV S  IY+KFNV+SGTSM+
Sbjct: 486 SVARYSSRGPSESCPLVLKPDIMAPGDAILASWPQKVAATDVYSRPIYSKFNVLSGTSMA 545

Query: 553 CPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSGH 612
           CPHAA VAALLKG H  WSPAAIRSAMMTTA VVDNTQT IKD+GNKNK ATPLAMGSGH
Sbjct: 546 CPHAAGVAALLKGAHPGWSPAAIRSAMMTTADVVDNTQTLIKDLGNKNKVATPLAMGSGH 605

Query: 613 VNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFIII 672
           VNPNKAIDP LIYD+GI+DY NLLCALNYTKNQIQTITRS SNDC+ PSLDLNYPSFII 
Sbjct: 606 VNPNKAIDPGLIYDIGIEDYTNLLCALNYTKNQIQTITRSPSNDCEKPSLDLNYPSFIIT 665

Query: 673 VNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 730
           VN+SDS++ +REI  EFKR +T IGEK ATY AK+  MKGL V V+PK L+FKRKNQ LS
Sbjct: 666 VNASDSESGRREISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPKMLKFKRKNQNLS 725

BLAST of Lsi08G015260 vs. NCBI nr
Match: XP_038885776.1 (subtilisin-like protease SBT3 [Benincasa hispida])

HSP 1 Score: 1238.0 bits (3202), Expect = 0.0e+00
Identity = 637/770 (82.73%), Postives = 672/770 (87.27%), Query Frame = 0

Query: 10  MAARLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLL 69
           MAA LYFWFSLIPI  LCP LTETD YIVHMNSAAMPKPFA+RHSWYSATISSVLH+S  
Sbjct: 1   MAAGLYFWFSLIPILWLCPILTETDNYIVHMNSAAMPKPFASRHSWYSATISSVLHSS-- 60

Query: 70  DTTISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTH 129
               SSSSS+S  SKL H YNHAI GFSAS +PSELEALKNSPGYVSSVLDSSVRVDTTH
Sbjct: 61  ---SSSSSSASSSSKLTHSYNHAIDGFSASLSPSELEALKNSPGYVSSVLDSSVRVDTTH 120

Query: 130 SSHFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTH 189
           SSHFLGL+SNFGLLPISNYGSDVIIGFVDTGIWPE ESFNDDGMSEIPSRWKGECE GTH
Sbjct: 121 SSHFLGLSSNFGLLPISNYGSDVIIGFVDTGIWPEGESFNDDGMSEIPSRWKGECERGTH 180

Query: 190 FNTSLCNKKLIGARFFNKGLIAKFPNLTIS------------------------------ 249
           FN SLCNKKLIGARFFNKGL+AKFPN+TIS                              
Sbjct: 181 FNASLCNKKLIGARFFNKGLLAKFPNITISMNSTRDTIGHGTHTATIAAGSYVKEASFFG 240

Query: 250 -------------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYED 309
                              AIWEE N+V DVIAAIDQAISDGVDVISLSLGLDGVPLY+D
Sbjct: 241 YGRGTARGVAPRARIAIYKAIWEEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYQD 300

Query: 310 PVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 369
           PVAIATFAAVERGIFVAT+ GNNGP+LGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGV+
Sbjct: 301 PVAIATFAAVERGIFVATTTGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVT 360

Query: 370 ILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDG-YSLSSQVDNIQS 429
           ILGSSLFPLNSTMDVSPLPIVFMGGC NLKKLKRIGYKI VC+DKD  YSLSSQVDN+QS
Sbjct: 361 ILGSSLFPLNSTMDVSPLPIVFMGGCQNLKKLKRIGYKIAVCDDKDEYYSLSSQVDNVQS 420

Query: 430 AKVALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTK 489
           A VALGVFISNISDWDNLIQTSFPSIFLSP++GNIIKDY+K+ SDPKAEVNFHKT+LGTK
Sbjct: 421 ANVALGVFISNISDWDNLIQTSFPSIFLSPYNGNIIKDYIKKSSDPKAEVNFHKTILGTK 480

Query: 490 PAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGT 549
           PAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWP NVAAM V S  IYTKFN+MSGT
Sbjct: 481 PAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPQNVAAMYVYSDPIYTKFNIMSGT 540

Query: 550 SMSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMG 609
           SMSCPHAA VAALLK VHH WSPAAIRSAMMTTA VVDN QTSIKDIGNKN+FA+PLAMG
Sbjct: 541 SMSCPHAAGVAALLKVVHHGWSPAAIRSAMMTTADVVDNNQTSIKDIGNKNRFASPLAMG 600

Query: 610 SGHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSF 669
           SGH+NPNKAIDP L+YDMGIQDYVNLLCALNYTK+QIQTITR  SNDC+NPS+DLNYPSF
Sbjct: 601 SGHINPNKAIDPGLVYDMGIQDYVNLLCALNYTKSQIQTITRLDSNDCENPSMDLNYPSF 660

Query: 670 IIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQ 729
           IIIVN+S+SKT K EILGEFKRTLTKIGEK+ATYEAKLRGMKG KV V PKKLEFKRKNQ
Sbjct: 661 IIIVNTSNSKTSKGEILGEFKRTLTKIGEKRATYEAKLRGMKGFKVGVNPKKLEFKRKNQ 720

BLAST of Lsi08G015260 vs. NCBI nr
Match: XP_008444560.1 (PREDICTED: subtilisin-like protease SBT1.9 [Cucumis melo] >KAA0060951.1 subtilisin-like protease SBT1.9 [Cucumis melo var. makuwa])

HSP 1 Score: 1209.1 bits (3127), Expect = 0.0e+00
Identity = 614/765 (80.26%), Postives = 662/765 (86.54%), Query Frame = 0

Query: 10  MAARLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLL 69
           MA RLYFWFSL PIF LCP LTET  YIVHMNSAAMPKPFA+RHSWYSATISSVLH+S  
Sbjct: 1   MAVRLYFWFSLTPIFWLCPILTETHNYIVHMNSAAMPKPFASRHSWYSATISSVLHSSSS 60

Query: 70  DTTISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTH 129
            ++ SSSSSSSFPSKLIH YNHAISGF AS TPS+LEALKNSPGY+SSVLDSSV VDTTH
Sbjct: 61  SSSSSSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 130 SSHFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTH 189
           SSHFLGL+SN GLLPIS YGSDVIIGFVDTGIWP+SESFNDDGMSEIPSRWKGECES TH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFNDDGMSEIPSRWKGECESSTH 180

Query: 190 FNTSLCNKKLIGARFFNKGLIAKFPNLTIS------------------------------ 249
           FN S CN KLIGARFFNKGLIA+FPN TIS                              
Sbjct: 181 FNVSFCNNKLIGARFFNKGLIAQFPNATISMNSTRDTIGHGTHTSTIAAGSYVKEASFFG 240

Query: 250 -------------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYED 309
                              AIW+E NSV DV+AAIDQAISDGVDVISLS+G+DG+PLY D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWDEGNSVSDVVAAIDQAISDGVDVISLSIGIDGIPLYND 300

Query: 310 PVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 369
           PV+IATFAAVERGIFVATSAGNNGP+LGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVSIATFAAVERGIFVATSAGNNGPQLGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 360

Query: 370 ILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSA 429
           +LGSSLFPLN+TM +SPLPIVFMGGC NLKKLKRIGYKIVVCED DGYSL+SQVDN+Q+A
Sbjct: 361 VLGSSLFPLNTTMGLSPLPIVFMGGCQNLKKLKRIGYKIVVCEDNDGYSLTSQVDNVQTA 420

Query: 430 KVALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKP 489
           KV LG+FISNI DWDNLIQT FPSIF++P+HGNIIKDY+ + SDPKAEVNFHKT+LGTKP
Sbjct: 421 KVTLGIFISNIFDWDNLIQTPFPSIFVNPYHGNIIKDYIHKSSDPKAEVNFHKTILGTKP 480

Query: 490 APSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTS 549
           AP+VARYSSRGPS SCPFVLKPDIMAPGDAILASWP N+ AMDVNST IYTKFNV+SGTS
Sbjct: 481 APTVARYSSRGPSQSCPFVLKPDIMAPGDAILASWPQNIPAMDVNSTPIYTKFNVISGTS 540

Query: 550 MSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGS 609
           MSCPHAA VAALLKG H +WSPAAIRSAMMTTA VVDNTQTSIKDIGN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADVVDNTQTSIKDIGNNNKFATPLAMGS 600

Query: 610 GHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFI 669
           GHVNPNKA+DPDLIYD+GIQDYVN+LCALNYT+NQI+ ITRS SN+C+NPSLDLNYPSFI
Sbjct: 601 GHVNPNKAVDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 670 IIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQK 726
           II NSSDSKT KR+ILGEFKRTLTKIGE +ATYEAKLRGMKG KVRVKP+ LEFKRKNQK
Sbjct: 661 IIANSSDSKTGKRKILGEFKRTLTKIGENRATYEAKLRGMKGFKVRVKPRILEFKRKNQK 720

BLAST of Lsi08G015260 vs. NCBI nr
Match: XP_004143027.3 (subtilisin-like protease SBT3 [Cucumis sativus] >KGN62450.1 hypothetical protein Csa_018640 [Cucumis sativus])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 600/765 (78.43%), Postives = 649/765 (84.84%), Query Frame = 0

Query: 10  MAARLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLL 69
           MAARLYFWFSLIPIF LCP LTET  YIVHMNSAAMPKPFA+RHSWYSATISS+LH+   
Sbjct: 1   MAARLYFWFSLIPIFWLCPILTETRNYIVHMNSAAMPKPFASRHSWYSATISSLLHS--- 60

Query: 70  DTTISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTH 129
               SSSSSSSFPSKLIH YNHAISGF AS TPS+LEALKNSPGY+SSVLDSSV VDTTH
Sbjct: 61  ----SSSSSSSFPSKLIHTYNHAISGFCASLTPSQLEALKNSPGYLSSVLDSSVHVDTTH 120

Query: 130 SSHFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTH 189
           SSHFLGL+SN GLLPIS YGSDVIIGFVDTGIWP+SESF DDGMSEIPS+WKGECES TH
Sbjct: 121 SSHFLGLSSNHGLLPISKYGSDVIIGFVDTGIWPDSESFIDDGMSEIPSKWKGECESSTH 180

Query: 190 FNTSLCNKKLIGARFFNKGLIAKFPNLTIS------------------------------ 249
           FN S CN KLIGARFFNKGLI+  P  TIS                              
Sbjct: 181 FNVSFCNNKLIGARFFNKGLISGLPKATISINSTRDTIGHGTHTSTTAAGSYIKEASFFG 240

Query: 250 -------------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYED 309
                              AIWEE NSV DV+AAIDQAISDGVDVISLS+G+DGVPLY+D
Sbjct: 241 YGRGTARGVAPRARVAIYKAIWEEGNSVSDVVAAIDQAISDGVDVISLSIGIDGVPLYDD 300

Query: 310 PVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVS 369
           PVAIATFAAVERGIFVATSAGNNGP+L TVHNGAPW+LNVAAGTMDRDFGGTITLSNGVS
Sbjct: 301 PVAIATFAAVERGIFVATSAGNNGPQLETVHNGAPWLLNVAAGTMDRDFGGTITLSNGVS 360

Query: 370 ILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSA 429
           +LGSSLFPLN T  +SPLPIVFMGGC NLKKL+R GYKIVVCED DGYSL+SQVDN+Q+A
Sbjct: 361 VLGSSLFPLNITTGLSPLPIVFMGGCQNLKKLRRTGYKIVVCEDSDGYSLTSQVDNVQTA 420

Query: 430 KVALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKP 489
            VALG+FISNI DWDNLIQT FPSIFL+P+HGNIIKDY+ + SDPKAEV FHKT+L TKP
Sbjct: 421 NVALGIFISNIFDWDNLIQTPFPSIFLNPYHGNIIKDYIHKSSDPKAEVTFHKTILRTKP 480

Query: 490 APSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTS 549
           AP VARYSSRGPS SCPFVLKPDIMAPGD ILASWP NV AMDVNST IY+KFNV+SGTS
Sbjct: 481 APMVARYSSRGPSQSCPFVLKPDIMAPGDTILASWPQNVPAMDVNSTPIYSKFNVISGTS 540

Query: 550 MSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGS 609
           MSCPHAA VAALLKG H +WSPAAIRSAMMTTA ++DNTQT IKD GN NKFATPLAMGS
Sbjct: 541 MSCPHAAGVAALLKGAHPQWSPAAIRSAMMTTADILDNTQTYIKDFGNNNKFATPLAMGS 600

Query: 610 GHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFI 669
           GHVNPNKAIDPDLIYD+GIQDYVN+LCALNYT+NQI+ ITRS SN+C+NPSLDLNYPSFI
Sbjct: 601 GHVNPNKAIDPDLIYDVGIQDYVNVLCALNYTENQIRIITRSDSNNCENPSLDLNYPSFI 660

Query: 670 IIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQK 726
           +IVNSSDSKTRKR+I GEFKRTLTKIGE +ATYEAKL GMKG KVRVKP KL FKRKNQK
Sbjct: 661 MIVNSSDSKTRKRKISGEFKRTLTKIGEHRATYEAKLTGMKGFKVRVKPNKLNFKRKNQK 720

BLAST of Lsi08G015260 vs. NCBI nr
Match: KAG6598336.1 (Subtilisin-like protease 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 579/770 (75.19%), Postives = 636/770 (82.60%), Query Frame = 0

Query: 13  RLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSV-LHTSLLDT 72
           R++FW SLI IF    TLT+TD YIVHM+ AAMPKPFAT HSWYSAT+SSV L TS L T
Sbjct: 6   RMFFWVSLIVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 73  TISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSS 132
           T SSSSSS  PSKLIH Y HAI+GF+A+ TPS+L+ALKNSPGYVSSV DSSVR DTTHSS
Sbjct: 66  TTSSSSSS--PSKLIHCYKHAINGFTANLTPSQLDALKNSPGYVSSVRDSSVRADTTHSS 125

Query: 133 HFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFN 192
           +FL L+ N GLLPISNYGSDVIIGFVDTG+WPESESFNDDG+ +IPSRWKGECESGTHFN
Sbjct: 126 NFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGIPKIPSRWKGECESGTHFN 185

Query: 193 TSLCNKKLIGARFFNKGLIAKFPNLTIS-------------------------------- 252
            SLCNKKLIG RFFNKGLIAKFPN+TIS                                
Sbjct: 186 ASLCNKKLIGGRFFNKGLIAKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG 245

Query: 253 -----------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 312
                            AIW+E N+V DVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV
Sbjct: 246 QGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 305

Query: 313 AIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSIL 372
           AIATFAA+ERGIFVATSAGN GP+ GTVH+GAPWVLNVAAGTMDRDFGGTITL+NGVS+L
Sbjct: 306 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVL 365

Query: 373 GSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSAKV 432
           GSSLFPLN  + +SPLPIVFMG CHNLKKLKR+G+KIVVCED D YSL  QVDN++SAK+
Sbjct: 366 GSSLFPLNLAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKI 425

Query: 433 ALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKPAP 492
           A+GVFISNISDWDNLIQTSFPSIFL+ +HGN+IKDY+K  S+PKA VNFHKT++GTKPAP
Sbjct: 426 AVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAP 485

Query: 493 SVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSMS 552
           SVARYSSRGPS SCPFVLKPDIMAPGDAILASWP NVAA DV S  IY+K+NV+SGTSM+
Sbjct: 486 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKYNVLSGTSMA 545

Query: 553 CPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSGH 612
           CPHAA VAALLKG H  WSPAAIRSAMMTTA +VDNTQT IKD+GNKNK ATPLAMGSGH
Sbjct: 546 CPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGH 605

Query: 613 VNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFIII 672
           VNPNKAIDP LIYDMGI+DY NLLCALNYTKNQIQTITRS SN C+ P LDLNYPSFII 
Sbjct: 606 VNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKPLLDLNYPSFIIT 665

Query: 673 VNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 730
           VN+SDS+T + EI  EFKR +T IGEK ATY AK+  MKGL V V+P KL+FKRKNQ LS
Sbjct: 666 VNASDSQTGRTEISREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLS 725

BLAST of Lsi08G015260 vs. NCBI nr
Match: XP_022962414.1 (subtilisin-like protease SBT1.9 [Cucurbita moschata])

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 576/770 (74.81%), Postives = 638/770 (82.86%), Query Frame = 0

Query: 13  RLYFWFSLIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSV-LHTSLLDT 72
           R++FW SL+ IF    TLT+TD YIVHM+ AAMPKPFAT HSWYSAT+SSV L TS L T
Sbjct: 6   RMFFWVSLMVIFLARSTLTQTDNYIVHMDLAAMPKPFATHHSWYSATLSSVLLDTSPLRT 65

Query: 73  TISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSS 132
           T +SSSSSS PSKLIH Y HAI+GF+AS TPS+L+ALKNSPGYVSS+ DSSVR DTTHSS
Sbjct: 66  T-TSSSSSSLPSKLIHCYKHAINGFTASLTPSQLDALKNSPGYVSSLRDSSVRADTTHSS 125

Query: 133 HFLGLNSNFGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFN 192
           +FL L+ N GLLPISNYGSDVIIGFVDTG+WPESESFNDDG+S+IPSRWKGECESGTHFN
Sbjct: 126 NFLALSPNSGLLPISNYGSDVIIGFVDTGVWPESESFNDDGISKIPSRWKGECESGTHFN 185

Query: 193 TSLCNKKLIGARFFNKGLIAKFPNLTIS-------------------------------- 252
            SLCNKKLIG RFFNKGLI+KFPN+TIS                                
Sbjct: 186 ASLCNKKLIGGRFFNKGLISKFPNVTISMNSTRDTNGHGTHTSTTAAGSYVKEASFFGYG 245

Query: 253 -----------------AIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 312
                            AIW+E N+V DVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV
Sbjct: 246 QGTARGVAPRARVAIYKAIWKEGNAVSDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPV 305

Query: 313 AIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSIL 372
           AIATFAA+ERGIFVATSAGN GP+ GTVH+GAPWVLNVAAGTMDRDFGGTITL+NGVS+L
Sbjct: 306 AIATFAAMERGIFVATSAGNKGPQFGTVHSGAPWVLNVAAGTMDRDFGGTITLTNGVSVL 365

Query: 373 GSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSAKV 432
           GSSLFPLNS + +SPLPIVFMG CHNLKKLKR+G+KIVVCED D YSL  QVDN++SAK+
Sbjct: 366 GSSLFPLNSAIALSPLPIVFMGQCHNLKKLKRVGFKIVVCEDSDEYSLDLQVDNVESAKI 425

Query: 433 ALGVFISNISDWDNLIQTSFPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKPAP 492
           A+GVFISNISDWDNLIQTSFPSIFL+ +HGN+IKDY+K  S+PKA VNFHKT++GTKPAP
Sbjct: 426 AVGVFISNISDWDNLIQTSFPSIFLNAYHGNVIKDYIKRSSNPKARVNFHKTIIGTKPAP 485

Query: 493 SVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSMS 552
           SVARYSSRGPS SCPFVLKPDIMAPGDAILASWP NVAA DV S  IY+KFNV+SGTSM+
Sbjct: 486 SVARYSSRGPSESCPFVLKPDIMAPGDAILASWPQNVAATDVYSRPIYSKFNVLSGTSMA 545

Query: 553 CPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSGH 612
           CPHAA VAALLKG H  WSPAAIRSAMMTTA +VDNTQT IKD+GNKNK ATPLAMGSGH
Sbjct: 546 CPHAAGVAALLKGAHPGWSPAAIRSAMMTTADIVDNTQTLIKDLGNKNKVATPLAMGSGH 605

Query: 613 VNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLDLNYPSFIII 672
           VNPNKAIDP LIYDMGI+DY NLLCALNYTKNQIQTITRS SN C+   LDLNYPSFI+ 
Sbjct: 606 VNPNKAIDPGLIYDMGIEDYTNLLCALNYTKNQIQTITRSTSNHCEKALLDLNYPSFIMT 665

Query: 673 VNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 730
           VN+SDS+T + E+  EFKR +T IGEK ATY AK+  MKGL V V+P KL+FKRKNQ LS
Sbjct: 666 VNASDSQTGRTEMSREFKRRVTNIGEKGATYRAKMTPMKGLVVTVEPNKLKFKRKNQNLS 725

BLAST of Lsi08G015260 vs. TAIR 10
Match: AT5G67090.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 513.1 bits (1320), Expect = 3.7e-145
Identity = 316/768 (41.15%), Postives = 430/768 (55.99%), Query Frame = 0

Query: 20  LIPIFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSS 79
           L+  F +     ET  YI+HM+ +A P PF+   SW+S T++SV+              +
Sbjct: 9   LVFSFFVAIVTAETSPYIIHMDLSAKPLPFSDHRSWFSTTLTSVI--------------T 68

Query: 80  SFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLNSN 139
           +   K+I+ Y  ++ GFSA  T SEL+ LK+ PGYVS   D  V++ TT S  F+GLNS 
Sbjct: 69  NRKPKIIYAYTDSVHGFSAVLTNSELQRLKHKPGYVSFTKDLPVKLHTTFSPKFIGLNST 128

Query: 140 FGLLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKL 199
            G  P+SNYG+ ++IG +DTGIWP+S SF+DDG+  +PS+WKG CE     ++SLCNKKL
Sbjct: 129 SGTWPVSNYGAGIVIGIIDTGIWPDSPSFHDDGVGSVPSKWKGACEFN---SSSLCNKKL 188

Query: 200 IGARFFNKGLIAKFPNL------------------------------------------- 259
           IGA+ FNKGL A  P+L                                           
Sbjct: 189 IGAKVFNKGLFANNPDLRETKIGQYSSPYDTIGHGTHVAAIAAGNHVKNASYFSYAQGTA 248

Query: 260 ----------TISAIWEESNSVLDVIAAIDQAISDGVDVISLSLGL--------DGVPLY 319
                        A WEE     DVIAAIDQAI DGV VISLSLGL        DG  L 
Sbjct: 249 SGIAPHAHLAIYKAAWEEGIYSSDVIAAIDQAIRDGVHVISLSLGLSFEDDDDNDGFGLE 308

Query: 320 EDPVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNG 379
            DP+A+A+FAA+++G+FV TS GN+GP   ++ NGAPW++ V AGT+ R F GT+T  N 
Sbjct: 309 NDPIAVASFAAIQKGVFVVTSGGNDGPYYWSLINGAPWIMTVGAGTIGRQFQGTLTFGNR 368

Query: 380 VSILGSSLFPLNSTMDVSPLPIVFMGGCHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQ 439
           VS    SLFP        P+  +  G   N    K +  +IVVC   +  ++ S++  I+
Sbjct: 369 VSFSFPSLFPGEFPSVQFPVTYIESGSVEN----KTLANRIVVC--NENINIGSKLHQIR 428

Query: 440 SAKVALGVFISN-ISDWDNLIQTSFPSIFLSPHHGNIIKDYL-KECSDPKAEVNFHKTVL 499
           S   A  V I++ + +  + I+  FP  F+   H   I+ Y     ++  A++ F KTV+
Sbjct: 429 STGAAAVVLITDKLLEEQDTIKFQFPVAFIGSKHRETIESYASSNKNNATAKLEFRKTVI 488

Query: 500 GTKPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVM 559
           GTKPAP V  YSSRGP  S P +LKPDI+APG  IL++WP         +  +++ FN++
Sbjct: 489 GTKPAPEVGTYSSRGPFTSFPQILKPDILAPGTLILSAWPSVEQITGTRALPLFSGFNLL 548

Query: 560 SGTSMSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPL 619
           +GTSM+ PH A VAAL+K VH  WSP+AI+SA+MTTA  +DN                PL
Sbjct: 549 TGTSMAAPHVAGVAALIKQVHPNWSPSAIKSAIMTTALTLDN----------------PL 608

Query: 620 AMGSGHVNPNKAIDPDLIYDMGIQDYVNLLC-ALNYTKNQIQTITRSASND-CKNPSLDL 679
           A+G+GHV+ NK ++P LIYD   QD++N LC     ++  I  ITRS  +D CK PS  L
Sbjct: 609 AVGAGHVSTNKVLNPGLIYDTTPQDFINFLCHEAKQSRKLINIITRSNISDACKKPSPYL 668

Query: 680 NYPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEF 723
           NYPS I    S  S  +       FKRTLT +GE K +Y  ++RG+KGL V V+PKKL F
Sbjct: 669 NYPSIIAYFTSDQSSPK------IFKRTLTNVGEAKRSYIVRVRGLKGLNVVVEPKKLMF 728

BLAST of Lsi08G015260 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 6.2e-124
Identity = 276/774 (35.66%), Postives = 399/774 (51.55%), Query Frame = 0

Query: 23  IFCLCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSSSFP 82
           +F L  T T    YI+ +N +  P+ F T H WY++ ++S                    
Sbjct: 18  LFLLLHT-TAKKTYIIRVNHSDKPESFLTHHDWYTSQLNS-------------------E 77

Query: 83  SKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVL-DSSVRVDTTHSSHFLGLNSNFG 142
           S L++ Y  +  GFSA    +E ++L +S   +  +  D    + TT +  FLGLNS FG
Sbjct: 78  SSLLYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFG 137

Query: 143 LLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKLIG 202
           +  + +  + VIIG +DTG+WPES SF+D  M EIPS+WKGECESG+ F++ LCNKKLIG
Sbjct: 138 VHDLGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIG 197

Query: 203 ARFFNK----------------------------------------------------GL 262
           AR F+K                                                    G+
Sbjct: 198 ARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGM 257

Query: 263 IAKFPNLTISAIWEESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAA 322
             +    T    W       D++AA+D+AI DGVDV+SLSLG    P Y D +AI  F+A
Sbjct: 258 ATRARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSA 317

Query: 323 VERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSILGSSLFPL 382
           +ERG+FV+ SAGN+GP   +V N APWV+ V AGT+DRDF     L NG  + G SL+  
Sbjct: 318 MERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYS- 377

Query: 383 NSTMDVSPLPIVFMGGCHNLKKLKRIGY--------KIVVCEDKDGYSLSSQVDNIQSAK 442
              M   PL +V+  G  +   L   G         KIVVC+   G +   +   +    
Sbjct: 378 GVGMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCD--RGVNARVEKGAVVRDA 437

Query: 443 VALGVFISN-ISDWDNLIQTS--FPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGT 502
             LG+ ++N  +  + L+  S   P+I +    G+++++Y+K  S P A + F  TVL  
Sbjct: 438 GGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDV 497

Query: 503 KPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSG 562
           KP+P VA +SSRGP+   P +LKPD++ PG  ILA W   +    ++     T+FN+MSG
Sbjct: 498 KPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSG 557

Query: 563 TSMSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAM 622
           TSMSCPH + +A LLK  H  WSP+AI+SA+MTTA V+DNT   + D  + N  + P A 
Sbjct: 558 TSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAAD-NSLSNPYAH 617

Query: 623 GSGHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSLD---LN 682
           GSGHV+P KA+ P L+YD+  ++Y+  LC+L+YT + I  I +  S +C     D   LN
Sbjct: 618 GSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLN 677

Query: 683 YPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFK 730
           YPSF ++         KR +   + R +T +G   + Y+  + G   + + VKP KL FK
Sbjct: 678 YPSFSVLFGG------KRVV--RYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFK 737

BLAST of Lsi08G015260 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 425.2 bits (1092), Expect = 1.0e-118
Identity = 279/767 (36.38%), Postives = 401/767 (52.28%), Query Frame = 0

Query: 36  YIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSSSFPSKLIHIYNHAISG 95
           YIVH++  A P  F T   WY+++++S+               +S P  +IH Y+    G
Sbjct: 28  YIVHVDHEAKPSIFPTHFHWYTSSLASL---------------TSSPPSIIHTYDTVFHG 87

Query: 96  FSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLNS--NFGLLPISNYGSDVI 155
           FSA  T  +   L + P  +S + +    + TT S  FLGL S    GLL  S++GSD++
Sbjct: 88  FSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLV 147

Query: 156 IGFVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKLIGARFFNKGLIAK- 215
           IG +DTG+WPE  SF+D G+  +P +WKG+C +   F  S CN+KL+GARFF  G  A  
Sbjct: 148 IGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATN 207

Query: 216 -------------------------------FPNLTI-------------------SAIW 275
                                          FP  T+                      W
Sbjct: 208 GKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW 267

Query: 276 EESNSVLDVIAAIDQAISDGVDVISLSLGLDGVPLYEDPVAIATFAAVERGIFVATSAGN 335
                  D++AA D A++DGVDVISLS+G   VP Y D +AI  F A++RGIFV+ SAGN
Sbjct: 268 NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGN 327

Query: 336 NGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSILGSSLFPLNSTMDVSPLPIVF 395
            GP   TV N APW+  V AGT+DRDF   + L NG  I G S++           P+V+
Sbjct: 328 GGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVY 387

Query: 396 ----MGGCHNLKKL--------KRIGYKIVVCEDKDGYSLSSQVDNIQSAKVALGVFISN 455
               +GG      L          +  KIV+C+   G +  +    I      LG+ I+N
Sbjct: 388 GGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCD--RGINSRATKGEIVRKNGGLGMIIAN 447

Query: 456 -ISDWDNLIQTS--FPSIFLSPHHGNIIKDYLKECS------DPKAEVNFHKTVLGTKPA 515
            + D + L+      P+  +    G+ I+ Y+ E S       P A + F  T LG +PA
Sbjct: 448 GVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPA 507

Query: 516 PSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSM 575
           P VA +S+RGP+   P +LKPD++APG  ILA+WP  +    V S    T+FN++SGTSM
Sbjct: 508 PVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSM 567

Query: 576 SCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSG 635
           +CPH + +AALLK  H  WSPAAIRSA++TTA  VDN+   + D    N  ++ +  GSG
Sbjct: 568 ACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT-SSVMDYGSG 627

Query: 636 HVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASNDCKNPSL-----DLNY 695
           HV+P KA+DP L+YD+   DY+N LC  NYT+  I TITR  + DC          +LNY
Sbjct: 628 HVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQA-DCDGARRAGHVGNLNY 687

Query: 696 PSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKR 722
           PSF ++      +  + ++   F RT+T +G+  + YE K+R  +G  V V+P+KL F+R
Sbjct: 688 PSFSVVF----QQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRR 747

BLAST of Lsi08G015260 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 423.3 bits (1087), Expect = 3.9e-118
Identity = 274/761 (36.01%), Postives = 401/761 (52.69%), Query Frame = 0

Query: 36  YIVHMNSAAMPKPFATRHSWYSATISSVLHTSLLDTTISSSSSSSFPSKLIHIYNHAISG 95
           +I  ++  +MP  F T + WYS   +                     S+++H+Y+    G
Sbjct: 28  FIFRIDGGSMPSIFPTHYHWYSTEFAE-------------------ESRIVHVYHTVFHG 87

Query: 96  FSASFTPSELEALKNSPGYVSSVLDSSVRVDTTHSSHFLGLNSNFGLLPISNYGSDVIIG 155
           FSA  TP E + L+N P  ++   D    + TT S  FLGL +  GL   S+YGSDVIIG
Sbjct: 88  FSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKGLWSESDYGSDVIIG 147

Query: 156 FVDTGIWPESESFNDDGMSEIPSRWKGECESGTHFNTSLCNKKLIGARFFNKGL------ 215
             DTGIWPE  SF+D  +  IP RW+G CESG  F+   CN+K+IGARFF KG       
Sbjct: 148 VFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKIIGARFFAKGQQAAVIG 207

Query: 216 -----------------------------------------IAK--FPNLTISA---IWE 275
                                                    +AK   P   I+A    W+
Sbjct: 208 GINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAKGVAPKARIAAYKVCWK 267

Query: 276 ESNSV-LDVIAAIDQAISDGVDVISLSL-GLDGV--PLYEDPVAIATFAAVERGIFVATS 335
           +S  +  D++AA D A+ DGVDVIS+S+ G DG+  P Y DP+AI ++ A  +GIFV++S
Sbjct: 268 DSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSS 327

Query: 336 AGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTITLSNGVSILGSSLF---PLNSTMDVS 395
           AGN GP   +V N APWV  V A T+DR+F     L +G  + G SL+   PLN  M   
Sbjct: 328 AGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRGVSLYAGVPLNGRM--- 387

Query: 396 PLPIVFMGG---------CHNLKKLKRIGYKIVVCEDKDGYSLSSQVDNIQSAKVALGVF 455
             P+V+ G            N    K++  KIV+C+      ++  +   ++  V + + 
Sbjct: 388 -FPVVYPGKSGMSSASLCMENTLDPKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGM-IL 447

Query: 456 ISNISDWDNLIQTS--FPSIFLSPHHGNIIKDYLKECSDPKAEVNFHKTVLGTKPAPSVA 515
            +  S+ + L+  +   P+  +  + G+ IK Y     +P A ++F  T++G KPAP +A
Sbjct: 448 ANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIA 507

Query: 516 RYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAAMDVNSTQIYTKFNVMSGTSMSCPH 575
            +S RGP+   P +LKPD++APG  ILA+W   V    + S    T+FN++SGTSM+CPH
Sbjct: 508 SFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPH 567

Query: 576 AAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQTSIKDIGNKNKFATPLAMGSGHVNP 635
            +  AALLK  H  WSPA IRSAMMTT  +VDN+  S+ D  +  K ATP   GSGH+N 
Sbjct: 568 VSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLID-ESTGKSATPYDYGSGHLNL 627

Query: 636 NKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITRSASN--DCKNPSL-DLNYPSFIII 695
            +A++P L+YD+   DY+  LC++ Y    IQ ITR+       + PS  +LNYPS   +
Sbjct: 628 GRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAV 687

Query: 696 VNSSDSKTRKREILGEFKRTLTKIGEKKATYEAKLRGMKGLKVRVKPKKLEFKRKNQKLS 722
             ++      + ++    RT T +G+ +A Y A++   +G+ V VKP +L F    ++ S
Sbjct: 688 FPTNRRGLVSKTVI----RTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFTSAVKRRS 747

BLAST of Lsi08G015260 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 417.5 bits (1072), Expect = 2.1e-116
Identity = 279/788 (35.41%), Postives = 413/788 (52.41%), Query Frame = 0

Query: 8   SSMAARLYFWFSLIPIFC-LCPTLTETDIYIVHMNSAAMPKPFATRHSWYSATISSVLHT 67
           SS  +   F+  L   FC +  + ++   YIVHM  + MP  F    +WY +++ S+   
Sbjct: 3   SSFLSSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSI--- 62

Query: 68  SLLDTTISSSSSSSFPSKLIHIYNHAISGFSASFTPSELEALKNSPGYVSSVLDSSVRVD 127
                        S  ++L++ Y +AI GFS   T  E ++L   PG +S + +    + 
Sbjct: 63  -------------SDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELH 122

Query: 128 TTHSSHFLGLNSNFG-LLPISNYGSDVIIGFVDTGIWPESESFNDDGMSEIPSRWKGECE 187
           TT +  FLGL+ +   L P +   SDV++G +DTG+WPES+S++D+G   IPS WKG CE
Sbjct: 123 TTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCE 182

Query: 188 SGTHFNTSLCNKKLIGARFFNKGLIAKF---------------------PNLTISAIWEE 247
           +GT+F  SLCN+KLIGARFF +G  +                        + T +    E
Sbjct: 183 AGTNFTASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVE 242

Query: 248 SNSVL------------------------------DVIAAIDQAISDGVDVISLSLGLDG 307
             S+L                              D++AAID+AI+D V+V+S+SLG   
Sbjct: 243 GASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGM 302

Query: 308 VPLYEDPVAIATFAAVERGIFVATSAGNNGPELGTVHNGAPWVLNVAAGTMDRDFGGTIT 367
              Y D VAI  FAA+ERGI V+ SAGN GP   ++ N APW+  V AGT+DRDF     
Sbjct: 303 SDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 362

Query: 368 LSNGVSILGSSLFPLNSTMDVSPLPIVFMGGCHNLKK----------LKRIGYKIVVCED 427
           L NG +  G SLF   +  D   LP ++ G   N              +++  KIV+C+ 
Sbjct: 363 LGNGKNFTGVSLFKGEALPD-KLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCD- 422

Query: 428 KDGYSLSSQVDNIQSAKVALGVFISN-ISDWDNLIQTS--FPSIFLSPHHGNIIKDYLKE 487
             G +   Q  ++  A   +G+ ++N  ++ + L+  +   P+  +    G+II+ Y+  
Sbjct: 423 -RGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTT 482

Query: 488 CSDPKAEVNFHKTVLGTKPAPSVARYSSRGPSMSCPFVLKPDIMAPGDAILASWPHNVAA 547
             +P A ++   TV+G KP+P VA +SSRGP+   P +LKPD++APG  ILA+W      
Sbjct: 483 DPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGP 542

Query: 548 MDVNSTQIYTKFNVMSGTSMSCPHAAAVAALLKGVHHRWSPAAIRSAMMTTAGVVDNTQT 607
             + S     +FN++SGTSMSCPH + +AALLK VH  WSPAAIRSA+MTTA        
Sbjct: 543 TGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGK 602

Query: 608 SIKDIGNKNKFATPLAMGSGHVNPNKAIDPDLIYDMGIQDYVNLLCALNYTKNQIQTITR 667
            + DI    K +TP   G+GHV+P  A +P LIYD+  +DY+  LCALNYT  QI++++R
Sbjct: 603 PLLDIAT-GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR 662

Query: 668 SASNDCKNPS-----LDLNYPSFIIIVNSSDSKTRKREILGEFKRTLTKIGEKKATYEAK 723
              N   +PS      DLNYPSF + V+   +         ++ RT+T +G    TY  K
Sbjct: 663 --RNYTCDPSKSYSVADLNYPSFAVNVDGVGAY--------KYTRTVTSVG-GAGTYSVK 722