Lsi08G014800 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G014800
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	transcription factor HEC3-like
Location	chr08: 22953246 .. 22954131 (-)
RNA-Seq Expression	Lsi08G014800
Synteny	Lsi08G014800

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGACTATTATTATTAGTTTTTGAAATTGTAATAAATAATTGAGAGAGGGATTTGGCGATGGATGTTGAGCATGTAATTAAGTCGGGAGATGAGATGGATATTGTGAGTGTGATTCAGAATATGGGAAAATTTGCAGAGAATTTAGAATATAGTGAAGTGGCAGCTTCGGCTTTTGCTTTTGGTTTTGGTAGTTCCAATATTCCCACTCCCACTTCACAACCCATTTTTCCTTCTTTTAATAATCTCCCACCATCCACCCTTCCCATCAATAACGCCACTCCACCACCGTCTTTTCCACCCTATTCCGCCGCTGCCCGGTGGAGATCCCATCTTCCCGCCACTCGAAATTCCCGCATGGTACGTATCATACATACTCTTAAACTTACAGTCATTTTAACCATTTCGTTCGTCTAAGTTTTATGTTTTTAAGATTATGGGGTTAATTAATTAATTAATTATAATAATGATGATTATAGATGAAGGGGGAAGAATCAGAATCAATGGGAGCAATGAGGGAGATGATATACAGAATAGCAGCGATGCAGCCAATAGAGATAGACCCAGAAGCGGTGAAACCACCAAAGCGCCGGAATGTTAAAATTTCAAAAGATCCACAGAGCGTAGCGGCCAGGCTTAGAAGAGAGAGAATAAGTGAGAGAATTCGAATTCTTCAAAGAATTGTCCCCGGCGGCACCAAAATGGACACCGCCTCCATGTTAGACGAAGCCATTCATTACCTCAAGTTCTTGAAAAACCAACTCCATTCACTCCAAATTGCCGCCGCCACCACCAACCGCCCCGCCGCCTCCGCCTATCCTTTCCCCACCACGATGTTCTCCGGTGGGACTACTAATTTTAAACATCACCACGTGGAGGATGCTTGA

mRNA sequence

TTTGACTATTATTATTAGTTTTTGAAATTGTAATAAATAATTGAGAGAGGGATTTGGCGATGGATGTTGAGCATGTAATTAAGTCGGGAGATGAGATGGATATTGTGAGTGTGATTCAGAATATGGGAAAATTTGCAGAGAATTTAGAATATAGTGAAGTGGCAGCTTCGGCTTTTGCTTTTGGTTTTGGTAGTTCCAATATTCCCACTCCCACTTCACAACCCATTTTTCCTTCTTTTAATAATCTCCCACCATCCACCCTTCCCATCAATAACGCCACTCCACCACCGTCTTTTCCACCCTATTCCGCCGCTGCCCGGTGGAGATCCCATCTTCCCGCCACTCGAAATTCCCGCATGATGAAGGGGGAAGAATCAGAATCAATGGGAGCAATGAGGGAGATGATATACAGAATAGCAGCGATGCAGCCAATAGAGATAGACCCAGAAGCGGTGAAACCACCAAAGCGCCGGAATGTTAAAATTTCAAAAGATCCACAGAGCGTAGCGGCCAGGCTTAGAAGAGAGAGAATAAGTGAGAGAATTCGAATTCTTCAAAGAATTGTCCCCGGCGGCACCAAAATGGACACCGCCTCCATGTTAGACGAAGCCATTCATTACCTCAAGTTCTTGAAAAACCAACTCCATTCACTCCAAATTGCCGCCGCCACCACCAACCGCCCCGCCGCCTCCGCCTATCCTTTCCCCACCACGATGTTCTCCGGTGGGACTACTAATTTTAAACATCACCACGTGGAGGATGCTTGA

Coding sequence (CDS)

ATGGATGTTGAGCATGTAATTAAGTCGGGAGATGAGATGGATATTGTGAGTGTGATTCAGAATATGGGAAAATTTGCAGAGAATTTAGAATATAGTGAAGTGGCAGCTTCGGCTTTTGCTTTTGGTTTTGGTAGTTCCAATATTCCCACTCCCACTTCACAACCCATTTTTCCTTCTTTTAATAATCTCCCACCATCCACCCTTCCCATCAATAACGCCACTCCACCACCGTCTTTTCCACCCTATTCCGCCGCTGCCCGGTGGAGATCCCATCTTCCCGCCACTCGAAATTCCCGCATGATGAAGGGGGAAGAATCAGAATCAATGGGAGCAATGAGGGAGATGATATACAGAATAGCAGCGATGCAGCCAATAGAGATAGACCCAGAAGCGGTGAAACCACCAAAGCGCCGGAATGTTAAAATTTCAAAAGATCCACAGAGCGTAGCGGCCAGGCTTAGAAGAGAGAGAATAAGTGAGAGAATTCGAATTCTTCAAAGAATTGTCCCCGGCGGCACCAAAATGGACACCGCCTCCATGTTAGACGAAGCCATTCATTACCTCAAGTTCTTGAAAAACCAACTCCATTCACTCCAAATTGCCGCCGCCACCACCAACCGCCCCGCCGCCTCCGCCTATCCTTTCCCCACCACGATGTTCTCCGGTGGGACTACTAATTTTAAACATCACCACGTGGAGGATGCTTGA

Protein sequence

MDVEHVIKSGDEMDIVSVIQNMGKFAENLEYSEVAASAFAFGFGSSNIPTPTSQPIFPSFNNLPPSTLPINNATPPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNFKHHHVEDA

Homology

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BLAST of Lsi08G014800 vs. ExPASy Swiss-Prot
Match: Q9FHA7 (Transcription factor HEC1 OS=Arabidopsis thaliana OX=3702 GN=HEC1 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.4e-36
Identity = 87/132 (65.91%), Postives = 98/132 (74.24%), Query Frame = 0


Query: 74  TPPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEIDPEAVK 133
           T P S  P +A     S +   + +         +M AMREMI+RIA MQPI IDPEAVK
Sbjct: 64  TNPSSISPNTA----YSSVFLDKRNNSNNNNNGTNMAAMREMIFRIAVMQPIHIDPEAVK 123

Query: 134 PPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLKFLKN 193
           PPKRRNV+ISKDPQSVAAR RRERISERIRILQR+VPGGTKMDTASMLDEAIHY+KFLK 
Sbjct: 124 PPKRRNVRISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKK 183

Query: 194 QLHSLQIAAATT 206
           Q+ SL+  A  T
Sbjct: 184 QVQSLEEQAVVT 191

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BLAST of Lsi08G014800 vs. ExPASy Swiss-Prot
Match: Q9SND4 (Transcription factor HEC2 OS=Arabidopsis thaliana OX=3702 GN=HEC2 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 8.4e-35
Identity = 92/155 (59.35%), Postives = 106/155 (68.39%), Query Frame = 0


Query: 47  NIPTPTSQPIFPSFNNLPPSTLPINNATP--PPSFPPYSAAARWRSHLPATRNSRMMKGE 106
           NI   +     P F N   S LP +   P   P    +  A    S LP  R        
Sbjct: 33  NIMMLSESNTHPFFFNPTHSHLPFDQTMPHHQPGL-NFRYAPSPSSSLPEKRGG----CS 92

Query: 107 ESESMGAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRI 166
           ++ +M AMREMI+RIA MQPI IDPE+VKPPKR+NV+ISKDPQSVAAR RRERISERIRI
Sbjct: 93  DNANMAAMREMIFRIAVMQPIHIDPESVKPPKRKNVRISKDPQSVAARHRRERISERIRI 152

Query: 167 LQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQ 200
           LQR+VPGGTKMDTASMLDEAIHY+KFLK Q+ SL+
Sbjct: 153 LQRLVPGGTKMDTASMLDEAIHYVKFLKKQVQSLE 182

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BLAST of Lsi08G014800 vs. ExPASy Swiss-Prot
Match: Q9LXD8 (Transcription factor HEC3 OS=Arabidopsis thaliana OX=3702 GN=HEC3 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.0e-32
Identity = 82/141 (58.16%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 68  LPINNATPPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEI 127
           L I N      FPP S++    + L   +     + E  E +GAM+EM+Y+IAAMQ ++I
Sbjct: 51  LHIFNPFSSSHFPPLSSSLTTTTLLSGDQEDDEDEEEPLEELGAMKEMMYKIAAMQSVDI 110

Query: 128 DPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHY 187
           DP  VK PKRRNV+IS DPQSVAAR RRERISERIRILQR+VPGGTKMDTASMLDEAI Y
Sbjct: 111 DPATVKKPKRRNVRISDDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIRY 170

Query: 188 LKFLKNQLHSLQIAAATTNRP 209
           +KFLK Q+  L      T  P
Sbjct: 171 VKFLKRQIRLLNNNTGYTPPP 191

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BLAST of Lsi08G014800 vs. ExPASy Swiss-Prot
Match: O81313 (Transcription factor IND OS=Arabidopsis thaliana OX=3702 GN=IND PE=1 SV=3)

HSP 1 Score: 128.3 bits (321), Expect = 1.2e-28
Identity = 67/96 (69.79%), Postives = 75/96 (78.12%), Query Frame = 0


Query: 104 EESESMGAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIR 163
           E  E M AM+EM Y IA MQP++IDP  V  P RRNV+IS DPQ+V AR RRERISE+IR
Sbjct: 80  EYDEDMDAMKEMQYMIAVMQPVDIDPATVPKPNRRNVRISDDPQTVVARRRRERISEKIR 139

Query: 164 ILQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQ 200
           IL+RIVPGG KMDTASMLDEAI Y KFLK Q+  LQ
Sbjct: 140 ILKRIVPGGAKMDTASMLDEAIRYTKFLKRQVRILQ 175

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BLAST of Lsi08G014800 vs. ExPASy Swiss-Prot
Match: Q8S3D2 (Transcription factor bHLH87 OS=Arabidopsis thaliana OX=3702 GN=BHLH87 PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 8.4e-27
Identity = 61/95 (64.21%), Postives = 79/95 (83.16%), Query Frame = 0


Query: 105 ESESMGAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRI 164
           ++E++  M+EMIYR AA +P+    E V+ PKR+NVKIS DPQ+VAAR RRERISE+IR+
Sbjct: 238 DAEAIAQMKEMIYRAAAFRPVNFGLEIVEKPKRKNVKISTDPQTVAARQRRERISEKIRV 297

Query: 165 LQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQ 200
           LQ +VPGGTKMDTASMLDEA +YLKFL+ Q+ +L+
Sbjct: 298 LQTLVPGGTKMDTASMLDEAANYLKFLRAQVKALE 332

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BLAST of Lsi08G014800 vs. ExPASy TrEMBL
Match: A0A0A0LRA8 (BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G357190 PE=4 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.9e-74
Identity = 166/211 (78.67%), Postives = 177/211 (83.89%), Query Frame = 0


Query: 19  IQNMGKFAENLEYSEVAASAFAFGFGSSNIPTPTSQPIFPSFNNLPPSTLPINNAT---P 78
           I+ MGKF EN E +E+A S   FGFGSSNI T TS PIFPSFNN+PP+TL +NNAT    
Sbjct: 3   IEEMGKFGENFECNEIATS-LDFGFGSSNI-TSTSHPIFPSFNNIPPTTLSMNNATHAVA 62

Query: 79  PPSFPPYSAAARWRSHLPATRNSRMMKGE------ESESM-GAMREMIYRIAAMQPIEID 138
           PPSFPPYSAAARWRSH PATRNSRM  GE      ESESM G MREMIYRIAAMQPIEID
Sbjct: 63  PPSFPPYSAAARWRSHFPATRNSRMKAGEEEVEGSESESMGGGMREMIYRIAAMQPIEID 122

Query: 139 PEAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYL 198
           PEAVK PKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDEAIHYL
Sbjct: 123 PEAVKAPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDEAIHYL 182

Query: 199 KFLKNQLHSLQIAAA--TTNRPAASAYPFPT 218
           KFLKNQLHSLQIAAA  +++  ++SAY FPT
Sbjct: 183 KFLKNQLHSLQIAAASSSSSSSSSSAYAFPT 211

[Close]

BLAST of Lsi08G014800 vs. ExPASy TrEMBL
Match: A0A1S3BA85 (transcription factor HEC3-like OS=Cucumis melo OX=3656 GN=LOC103487880 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 6.8e-72
Identity = 164/233 (70.39%), Postives = 181/233 (77.68%), Query Frame = 0


Query: 19  IQNMGKFAENLEYSEVAASAFAFGFGSSNIPTPTSQPIFPSFNNLPPSTLPINNAT---P 78
           I+ MGKF EN EY+E+ A+   FGFGSSNI + TS  IFPSFNN+PP+TL +NNAT    
Sbjct: 3   IEEMGKFGENFEYNEI-ATTLDFGFGSSNIAS-TSHLIFPSFNNIPPTTLSMNNATHPVA 62

Query: 79  PPSFPPYSAAARWRSHLPATRNS-----RMMKGEESESM-GAMREMIYRIAAMQPIEIDP 138
           PPSFPPYSAAARWRSH PATRNS       ++G ESESM G MREMIYRIAAMQPIEIDP
Sbjct: 63  PPSFPPYSAAARWRSHFPATRNSGTTGEEEVEGSESESMGGGMREMIYRIAAMQPIEIDP 122

Query: 139 EAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 198
           EAVK PKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK
Sbjct: 123 EAVKAPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 182

Query: 199 FLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNF---------KHHHVE 234
           FLKNQLHSLQIAAA++   ++SAY FPT        +           HHH++
Sbjct: 183 FLKNQLHSLQIAAASS---SSSAYVFPTVAHYNNKQHLIHFPQFPTHPHHHLD 230

[Close]

BLAST of Lsi08G014800 vs. ExPASy TrEMBL
Match: A0A6J1KA10 (transcription factor HEC2-like OS=Cucurbita maxima OX=3661 GN=LOC111492009 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 1.4e-53
Identity = 138/234 (58.97%), Postives = 164/234 (70.09%), Query Frame = 0


Query: 16  VSVIQNMGKFAENLEYSEVAASAFAFG---FGSSNIPTPTSQPIFPSFNNLPPSTLPINN 75
           + V+  M  F+E  E++++ AS F+FG    G+++I      PI PS+     ST  +NN
Sbjct: 1   MDVLTQMENFSEYFEFTKLPASPFSFGSSNIGATSIRFQNPTPILPSY--ALSSTASMNN 60

Query: 76  AT---PPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEIDP 135
           AT    PP  PP+S+AA   SH            ++ +SMGAMREMIYRIAAMQPI+IDP
Sbjct: 61  ATHPLAPPCLPPHSSAATPNSHTK----------QQQDSMGAMREMIYRIAAMQPIQIDP 120

Query: 136 EAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 195
           EAVKPPKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDEAIHY+K
Sbjct: 121 EAVKPPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDEAIHYVK 180

Query: 196 FLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNFK--------HHHVEDA 236
           FLKNQ+  LQIAA   NRP +SA+ FP  M +GG +N+K        H HVEDA
Sbjct: 181 FLKNQVQCLQIAA--NNRP-SSAFGFPAAMSAGG-SNYKQFHFAQSGHEHVEDA 218

[Close]

BLAST of Lsi08G014800 vs. ExPASy TrEMBL
Match: A0A6J1HET7 (transcription factor HEC2-like OS=Cucurbita moschata OX=3662 GN=LOC111462825 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 5.6e-50
Identity = 135/234 (57.69%), Postives = 159/234 (67.95%), Query Frame = 0


Query: 13  MDIVSVIQNMGKFAENLEYSEVAASAFAFGFGSSNIPTPTSQPIFPSFNNLPPSTLPINN 72
           MD+++ ++N  +F E  ++    +  F         PTP   PI PS+     ST  +NN
Sbjct: 1   MDVLTQMENFSEFFEFTDFGSSTSHRF-------QNPTP---PILPSY--ALSSTASMNN 60

Query: 73  AT---PPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEIDP 132
           AT    PP  PP+S AA   SH            ++ +SMGAMREMIYRIAAMQPI+IDP
Sbjct: 61  ATHPLAPPCPPPHSPAATPNSHTK----------QQQDSMGAMREMIYRIAAMQPIQIDP 120

Query: 133 EAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 192
           EAVKPPKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDEAIHY+K
Sbjct: 121 EAVKPPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDEAIHYVK 180

Query: 193 FLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNFK--------HHHVEDA 236
           FLK+Q+ SLQIAA   NRP +SA+ FP  M +GG +N+K        H HVEDA
Sbjct: 181 FLKSQVQSLQIAA--NNRP-SSAFRFPAAMSAGG-SNYKQFQFAQSGHEHVEDA 208

[Close]

BLAST of Lsi08G014800 vs. ExPASy TrEMBL
Match: A0A5A7V0Q5 (Transcription factor HEC3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00430 PE=4 SV=1)

HSP 1 Score: 186.4 bits (472), Expect = 1.3e-43
Identity = 106/143 (74.13%), Postives = 116/143 (81.12%), Query Frame = 0


Query: 101 MKGEESESM-GAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERIS 160
           ++G ESESM G MREMIYRIAAMQPIEIDPEAVK PKRRNVKISKDPQSVAAR RRERIS
Sbjct: 53  VEGSESESMGGGMREMIYRIAAMQPIEIDPEAVKAPKRRNVKISKDPQSVAARHRRERIS 112

Query: 161 ERIRILQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQIAAATTNRPAASAYPFPTTM 220
           ERIRILQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQIAAA+++  ++SAY FPT  
Sbjct: 113 ERIRILQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQIAAASSS-SSSSAYVFPTVA 172

Query: 221 FSGGTTNF---------KHHHVE 234
                 +           HHH++
Sbjct: 173 HYNNKQHLIHFPQFPTHPHHHLD 194

[Close]

BLAST of Lsi08G014800 vs. NCBI nr
Match: XP_011650182.1 (transcription factor HEC3 [Cucumis sativus] >KAE8652076.1 hypothetical protein Csa_018561 [Cucumis sativus])

HSP 1 Score: 288.9 bits (738), Expect = 4.0e-74
Identity = 165/210 (78.57%), Postives = 178/210 (84.76%), Query Frame = 0


Query: 19  IQNMGKFAENLEYSEVAASAFAFGFGSSNIPTPTSQPIFPSFNNLPPSTLPINNAT---P 78
           I+ MGKF EN E +E+A S   FGFGSSNI T TS PIFPSFNN+PP+TL +NNAT    
Sbjct: 3   IEEMGKFGENFECNEIATS-LDFGFGSSNI-TSTSHPIFPSFNNIPPTTLSMNNATHAVA 62

Query: 79  PPSFPPYSAAARWRSHLPATRNSRM-----MKGEESESM-GAMREMIYRIAAMQPIEIDP 138
           PPSFPPYSAAARWRSH PATRNSRM     ++G ESESM G MREMIYRIAAMQPIEIDP
Sbjct: 63  PPSFPPYSAAARWRSHFPATRNSRMAGEEEVEGSESESMGGGMREMIYRIAAMQPIEIDP 122

Query: 139 EAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 198
           EAVK PKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK
Sbjct: 123 EAVKAPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 182

Query: 199 FLKNQLHSLQIAAA--TTNRPAASAYPFPT 218
           FLKNQLHSLQIAAA  +++  ++SAY FPT
Sbjct: 183 FLKNQLHSLQIAAASSSSSSSSSSAYAFPT 210

[Close]

BLAST of Lsi08G014800 vs. NCBI nr
Match: XP_038886669.1 (transcription factor HEC2-like [Benincasa hispida])

HSP 1 Score: 282.3 bits (721), Expect = 3.7e-72
Identity = 174/242 (71.90%), Postives = 185/242 (76.45%), Query Frame = 0


Query: 16  VSVIQNMGKF--AENLEYSEVAASAFAFGFGSSNI----PTPTSQPIFPSFNNL-PPSTL 75
           +++  +MG+F  AENLE SEV   AFA  FGSSNI     T TS PIF SFNNL PPST 
Sbjct: 1   MNIEDDMGEFAVAENLELSEVGGLAFA--FGSSNIGVSSSTSTSYPIFSSFNNLPPPSTF 60

Query: 76  PINNAT---PPPSFPPYSAAARW-RSHLPATRNSRMM-KGEESESMGAMREMIYRIAAMQ 135
            +NN T    PPSFPPYS+A RW  SHLPAT  S M  + EESESMG MREMIYR+AAMQ
Sbjct: 61  SMNNPTQPVAPPSFPPYSSAPRWTSSHLPATPISAMKGEEEESESMGGMREMIYRMAAMQ 120

Query: 136 PIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDE 195
           PIEIDPE VKPPKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDE
Sbjct: 121 PIEIDPETVKPPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDE 180

Query: 196 AIHYLKFLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNFK----------HHHVE 236
           AIHYLKFLKNQLHSLQIAAATT       YPFPT M SGGTTN+K          H+HVE
Sbjct: 181 AIHYLKFLKNQLHSLQIAAATT-------YPFPTAMSSGGTTNYKHLFHFPQPSGHNHVE 233

[Close]

BLAST of Lsi08G014800 vs. NCBI nr
Match: XP_008444609.1 (PREDICTED: transcription factor HEC3-like [Cucumis melo])

HSP 1 Score: 280.4 bits (716), Expect = 1.4e-71
Identity = 164/233 (70.39%), Postives = 181/233 (77.68%), Query Frame = 0


Query: 19  IQNMGKFAENLEYSEVAASAFAFGFGSSNIPTPTSQPIFPSFNNLPPSTLPINNAT---P 78
           I+ MGKF EN EY+E+ A+   FGFGSSNI + TS  IFPSFNN+PP+TL +NNAT    
Sbjct: 3   IEEMGKFGENFEYNEI-ATTLDFGFGSSNIAS-TSHLIFPSFNNIPPTTLSMNNATHPVA 62

Query: 79  PPSFPPYSAAARWRSHLPATRNS-----RMMKGEESESM-GAMREMIYRIAAMQPIEIDP 138
           PPSFPPYSAAARWRSH PATRNS       ++G ESESM G MREMIYRIAAMQPIEIDP
Sbjct: 63  PPSFPPYSAAARWRSHFPATRNSGTTGEEEVEGSESESMGGGMREMIYRIAAMQPIEIDP 122

Query: 139 EAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 198
           EAVK PKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK
Sbjct: 123 EAVKAPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 182

Query: 199 FLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNF---------KHHHVE 234
           FLKNQLHSLQIAAA++   ++SAY FPT        +           HHH++
Sbjct: 183 FLKNQLHSLQIAAASS---SSSAYVFPTVAHYNNKQHLIHFPQFPTHPHHHLD 230

[Close]

BLAST of Lsi08G014800 vs. NCBI nr
Match: XP_022996919.1 (transcription factor HEC2-like [Cucurbita maxima])

HSP 1 Score: 219.5 bits (558), Expect = 3.0e-53
Identity = 138/234 (58.97%), Postives = 164/234 (70.09%), Query Frame = 0


Query: 16  VSVIQNMGKFAENLEYSEVAASAFAFG---FGSSNIPTPTSQPIFPSFNNLPPSTLPINN 75
           + V+  M  F+E  E++++ AS F+FG    G+++I      PI PS+     ST  +NN
Sbjct: 1   MDVLTQMENFSEYFEFTKLPASPFSFGSSNIGATSIRFQNPTPILPSY--ALSSTASMNN 60

Query: 76  AT---PPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEIDP 135
           AT    PP  PP+S+AA   SH            ++ +SMGAMREMIYRIAAMQPI+IDP
Sbjct: 61  ATHPLAPPCLPPHSSAATPNSHTK----------QQQDSMGAMREMIYRIAAMQPIQIDP 120

Query: 136 EAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLK 195
           EAVKPPKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDEAIHY+K
Sbjct: 121 EAVKPPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDEAIHYVK 180

Query: 196 FLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNFK--------HHHVEDA 236
           FLKNQ+  LQIAA   NRP +SA+ FP  M +GG +N+K        H HVEDA
Sbjct: 181 FLKNQVQCLQIAA--NNRP-SSAFGFPAAMSAGG-SNYKQFHFAQSGHEHVEDA 218

[Close]

BLAST of Lsi08G014800 vs. NCBI nr
Match: KAG7029335.1 (Transcription factor HEC1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 216.9 bits (551), Expect = 1.9e-52
Identity = 143/240 (59.58%), Postives = 166/240 (69.17%), Query Frame = 0


Query: 16  VSVIQNMGKFAENLEYSEVAASAFAFGFGSSNI---------PTPTSQPIFPSFNNLPPS 75
           + V+  M  F+E  E++E+ +S F+  FGSSNI         PTP   PI PS+     S
Sbjct: 1   MDVLTQMENFSEFFEFTELPSSPFS--FGSSNIGATSHRFQNPTP---PILPSY--ALSS 60

Query: 76  TLPINNAT---PPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQ 135
           T  +NNAT    PP  PP+S AA   SH            ++ +SMGAMREMIYRIAAMQ
Sbjct: 61  TASMNNATHPLAPPCPPPHSPAATPNSHTK----------QQQDSMGAMREMIYRIAAMQ 120

Query: 136 PIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDE 195
           PI+IDPEAVKPPKRRNVKISKDPQSVAAR RRERISERIRILQRIVPGGTKMDTASMLDE
Sbjct: 121 PIQIDPEAVKPPKRRNVKISKDPQSVAARHRRERISERIRILQRIVPGGTKMDTASMLDE 180

Query: 196 AIHYLKFLKNQLHSLQIAAATTNRPAASAYPFPTTMFSGGTTNFK--------HHHVEDA 236
           AIHY+KFLK+Q+ SLQIAA   NRP +SA+ FP  M +GG +N+K        H HVEDA
Sbjct: 181 AIHYVKFLKSQVQSLQIAA--NNRP-SSAFRFPAAMSAGG-SNYKQFQFAQSGHEHVEDA 219

[Close]

BLAST of Lsi08G014800 vs. TAIR 10
Match: AT5G67060.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.4e-37
Identity = 87/132 (65.91%), Postives = 98/132 (74.24%), Query Frame = 0


Query: 74  TPPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEIDPEAVK 133
           T P S  P +A     S +   + +         +M AMREMI+RIA MQPI IDPEAVK
Sbjct: 64  TNPSSISPNTA----YSSVFLDKRNNSNNNNNGTNMAAMREMIFRIAVMQPIHIDPEAVK 123

Query: 134 PPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHYLKFLKN 193
           PPKRRNV+ISKDPQSVAAR RRERISERIRILQR+VPGGTKMDTASMLDEAIHY+KFLK 
Sbjct: 124 PPKRRNVRISKDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIHYVKFLKK 183

Query: 194 QLHSLQIAAATT 206
           Q+ SL+  A  T
Sbjct: 184 QVQSLEEQAVVT 191

[Close]

BLAST of Lsi08G014800 vs. TAIR 10
Match: AT3G50330.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 6.0e-36
Identity = 92/155 (59.35%), Postives = 106/155 (68.39%), Query Frame = 0


Query: 47  NIPTPTSQPIFPSFNNLPPSTLPINNATP--PPSFPPYSAAARWRSHLPATRNSRMMKGE 106
           NI   +     P F N   S LP +   P   P    +  A    S LP  R        
Sbjct: 33  NIMMLSESNTHPFFFNPTHSHLPFDQTMPHHQPGL-NFRYAPSPSSSLPEKRGG----CS 92

Query: 107 ESESMGAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRI 166
           ++ +M AMREMI+RIA MQPI IDPE+VKPPKR+NV+ISKDPQSVAAR RRERISERIRI
Sbjct: 93  DNANMAAMREMIFRIAVMQPIHIDPESVKPPKRKNVRISKDPQSVAARHRRERISERIRI 152

Query: 167 LQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQ 200
           LQR+VPGGTKMDTASMLDEAIHY+KFLK Q+ SL+
Sbjct: 153 LQRLVPGGTKMDTASMLDEAIHYVKFLKKQVQSLE 182

[Close]

BLAST of Lsi08G014800 vs. TAIR 10
Match: AT5G09750.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 141.7 bits (356), Expect = 7.3e-34
Identity = 82/141 (58.16%), Postives = 97/141 (68.79%), Query Frame = 0


Query: 68  LPINNATPPPSFPPYSAAARWRSHLPATRNSRMMKGEESESMGAMREMIYRIAAMQPIEI 127
           L I N      FPP S++    + L   +     + E  E +GAM+EM+Y+IAAMQ ++I
Sbjct: 51  LHIFNPFSSSHFPPLSSSLTTTTLLSGDQEDDEDEEEPLEELGAMKEMMYKIAAMQSVDI 110

Query: 128 DPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRILQRIVPGGTKMDTASMLDEAIHY 187
           DP  VK PKRRNV+IS DPQSVAAR RRERISERIRILQR+VPGGTKMDTASMLDEAI Y
Sbjct: 111 DPATVKKPKRRNVRISDDPQSVAARHRRERISERIRILQRLVPGGTKMDTASMLDEAIRY 170

Query: 188 LKFLKNQLHSLQIAAATTNRP 209
           +KFLK Q+  L      T  P
Sbjct: 171 VKFLKRQIRLLNNNTGYTPPP 191

[Close]

BLAST of Lsi08G014800 vs. TAIR 10
Match: AT4G00120.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 8.4e-30
Identity = 67/96 (69.79%), Postives = 75/96 (78.12%), Query Frame = 0


Query: 104 EESESMGAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIR 163
           E  E M AM+EM Y IA MQP++IDP  V  P RRNV+IS DPQ+V AR RRERISE+IR
Sbjct: 80  EYDEDMDAMKEMQYMIAVMQPVDIDPATVPKPNRRNVRISDDPQTVVARRRRERISEKIR 139

Query: 164 ILQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQ 200
           IL+RIVPGG KMDTASMLDEAI Y KFLK Q+  LQ
Sbjct: 140 ILKRIVPGGAKMDTASMLDEAIRYTKFLKRQVRILQ 175

[Close]

BLAST of Lsi08G014800 vs. TAIR 10
Match: AT3G21330.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 122.1 bits (305), Expect = 6.0e-28
Identity = 61/95 (64.21%), Postives = 79/95 (83.16%), Query Frame = 0


Query: 105 ESESMGAMREMIYRIAAMQPIEIDPEAVKPPKRRNVKISKDPQSVAARLRRERISERIRI 164
           ++E++  M+EMIYR AA +P+    E V+ PKR+NVKIS DPQ+VAAR RRERISE+IR+
Sbjct: 238 DAEAIAQMKEMIYRAAAFRPVNFGLEIVEKPKRKNVKISTDPQTVAARQRRERISEKIRV 297

Query: 165 LQRIVPGGTKMDTASMLDEAIHYLKFLKNQLHSLQ 200
           LQ +VPGGTKMDTASMLDEA +YLKFL+ Q+ +L+
Sbjct: 298 LQTLVPGGTKMDTASMLDEAANYLKFLRAQVKALE 332

The following BLAST results are available for this feature:

BLAST of Lsi08G014800 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FHA7	3.4e-36	65.91	Transcription factor HEC1 OS=Arabidopsis thaliana OX=3702 GN=HEC1 PE=1 SV=1	[more]
Q9SND4	8.4e-35	59.35	Transcription factor HEC2 OS=Arabidopsis thaliana OX=3702 GN=HEC2 PE=1 SV=1	[more]
Q9LXD8	1.0e-32	58.16	Transcription factor HEC3 OS=Arabidopsis thaliana OX=3702 GN=HEC3 PE=1 SV=1	[more]
O81313	1.2e-28	69.79	Transcription factor IND OS=Arabidopsis thaliana OX=3702 GN=IND PE=1 SV=3	[more]
Q8S3D2	8.4e-27	64.21	Transcription factor bHLH87 OS=Arabidopsis thaliana OX=3702 GN=BHLH87 PE=1 SV=1	[more]

BLAST of Lsi08G014800 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRA8	1.9e-74	78.67	BHLH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G357190 PE=4 S...	[more]
A0A1S3BA85	6.8e-72	70.39	transcription factor HEC3-like OS=Cucumis melo OX=3656 GN=LOC103487880 PE=4 SV=1	[more]
A0A6J1KA10	1.4e-53	58.97	transcription factor HEC2-like OS=Cucurbita maxima OX=3661 GN=LOC111492009 PE=4 ...	[more]
A0A6J1HET7	5.6e-50	57.69	transcription factor HEC2-like OS=Cucurbita moschata OX=3662 GN=LOC111462825 PE=...	[more]
A0A5A7V0Q5	1.3e-43	74.13	Transcription factor HEC3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]

BLAST of Lsi08G014800 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650182.1	4.0e-74	78.57	transcription factor HEC3 [Cucumis sativus] >KAE8652076.1 hypothetical protein C...	[more]
XP_038886669.1	3.7e-72	71.90	transcription factor HEC2-like [Benincasa hispida]	[more]
XP_008444609.1	1.4e-71	70.39	PREDICTED: transcription factor HEC3-like [Cucumis melo]	[more]
XP_022996919.1	3.0e-53	58.97	transcription factor HEC2-like [Cucurbita maxima]	[more]
KAG7029335.1	1.9e-52	59.58	Transcription factor HEC1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lsi08G014800 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67060.1	2.4e-37	65.91	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT3G50330.1	6.0e-36	59.35	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G09750.1	7.3e-34	58.16	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT4G00120.1	8.4e-30	69.79	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT3G21330.1	6.0e-28	64.21	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 148..197 e-value: 8.9E-13 score: 58.4
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 152..191 e-value: 1.4E-6 score: 28.2
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 142..191 score: 15.186197
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 138..201 e-value: 9.8E-11 score: 43.2
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 146..204
None	No IPR available	PANTHER	PTHR45914:SF29	BASIC HELIX-LOOP-HELIX (BHLH)-LIKE PROTEIN	coord: 47..228
None	No IPR available	PANTHER	PTHR45914	TRANSCRIPTION FACTOR HEC3-RELATED	coord: 47..228

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G014800.1	Lsi08G014800.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046983	protein dimerization activity