Lsi08G014660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G014660
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	EF hand calcium-binding protein family
Location	chr08: 22797390 .. 22801316 (-)
RNA-Seq Expression	Lsi08G014660
Synteny	Lsi08G014660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGGTGATGGTGTCATCGATGAACATGAGTTGGACAAACTCGTCGACTATGTCATGAGGTTTCAGGCCCCAAAGGCCCCCAAGGGCCCCAAGCCCCCAAAAAAGCATTGTGACGATGGCAAATCTATTGTCTAATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAGTTTACACATTACGTATTACACATTACAAACATTTGAAAGTCTATACCTGCAAAGTTTACACATTATGTTAGTTGTTACGCTGTTGAATAGGGATTAAAAAATGTAGCATGCCATCTTTGACATGCTTAACTTTAACGTAATGACCGAATTTTCAACAACAGGGAGATCTATAAATGGGGTGTGCGGGAGAATATAGTTGTTTTGGGTGTGTGTGGAGAATAATATTCTTTTATTTGCAACTTGATCTACAATTAATTTATTTATTTATTTTTTTTTTGGAGAAAGTACAAAAGTCTTCCTTTATGTTAATTATTTATACCTCCTTTTAATTGGCACTATTTCATGTTTTTTTTTTTTTTTGTTTTGTTTACATGCACTTTTAGTGTCGAGGTGTGATGAGGGTGCTAAGATATGTCCTAGTTGATATATCCAGGTGCACCTACTAATCTTCTACCTTCGTATCTTGTTAAAAAAAAGAAAAACATTAACAAACTATTAACATTTTCATTCAAACTTTCAATTGCCCATTATTTAGGTATTTTGCTTCTTTTTTTTTTTTTTTTTCAAGTTTTGTTTTTGAAAGATATAAATCATGAATGTAAAATTTGATAACAATTTTTTTCCCTAAAAAACTAAGTACTTTTTTTTAGTGGTTTTAGGTTTTAGTTTTTATAAAAAACCAAAAATCAGTCAGTTTGTTACATACTTTTAGTTTTGTTTTTTTTTTTAAAAAAAAAAAGAATGAAGATCAAATCACAAATGTTTACATACTTAGATTTTATAATAAAAATAAGTATTTAGAATTTGATTCGATTATAATGAGATTATTTTGATGGTGTTTATGGCAAATTACAAGCCACAATCTTAATAAGAAATCTATTGGTATTGTTTACTTTCATATGGAATTGCAACGAAGTATAGATGATAATCAAAATGTCATTTTTATATTTTTAATTAATTGGAGTATCATTGTCTATTTTAATTTAACTATTAATTTTTATTTACTTGTTCAAATTTTTATACTTGAACAAAAAATTATATTAGAAAATTTTAAATTTTAAAAATATAATAGTCGTTATCTTAAATCTCACCCATCTGTTTTCAATATTTGTAAAATAAAAACATTATAATTTTCCAAACAAAATCTAAGATAATTAGAATTTTTTTTATTATTTATTTTTTTGTAATTAATGGATGATATGTAGAAAGAAATGTTTGTACAATATATATTCTGGTTCATGATAGACCACTTAAATAGCTAAATAATTCGTGAAAAACGATTCTTCTAGTTCGGTAGGCTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTGAAAAACAATAATAATAGTAATAACAACAACTTGATTCGGATACTTTATGGTTGAGTTTGAGTCTCCTTTTACTAGCCTAAATCGTGGCTAGCTTAAGGATATTATTTAAACACTTAGATTTAACGCATTATACAATTTAATTTTGAATATTTTGCCGGTTGAATTTTAACTAATTAATTGATCCATTCACGAGTAACTTTCTTTTCCAACTTATTAATTGATTAGTTTCACCGTTCACAGTATTTAGTTGCTTATGGTTTAACTCGTTAAGCTGCAGATGAGTTTTAATCTCATTTATTGATAAGATTTTTCTAGTTGACTTGACATTTGGTTGAGGGCAGATATGACCTCTTTTGTCTTAATTTTGATTGTATCTCACTATCTTGTTCTTCTAAAAGAAAATTTTAATTAATTATATTAAATTCCAAAGTTCAAATTTAATGTTAAATTCATCCAAATTGGAAGATTGAATTAAAATTGGAGTAAAATTACAATATTATGTTTAGTACATCCACGAATGTCGGAGATGCGATCTGTTATTACTACAGTGTGTTATATTAAAAAAAATTCAAATTTTGTATTTAGTACATATTTAAGACCTAATGCATACAAAATCAAAATTTAAGTTTACAATTTGTAAAGTAGATGGACTAAAATAAATACAGGTTTTAAAATTTAATGACTAGACTTGTTATTTAATTTTTTTTAATTATATAGATTTTTATGTACTACACAATTAAATGGTAATTCATATAATTGCTTGTTTTAATTTACTAAACAATTAAATATTCACTACACACATGCACGGTCAATATCAATATAATAGTAGAATTAAAAAAAAAAAAAAAAAAGGAAATATTTTCAAGGAATTGCATGAGGTCAGATTAATTTGGACTTGGATATGATATCTCTTGATGGATGCTGTACGGGAAACTATATTAGATTTGCTATCTAGTTTCGTCTGGTCTCGTAACCATGACTACTTTTTCTAGTTACGAACGGGTCACGTAGATATGCAATAAAGGCCTGATTAACATGAACAAACATTTTTGGATGGAATTCTCGTTTGTTGTCGATACGAAGATATCAATTGGGCTATATTAAACTAGCATGCTCAGTAACCCATATGTTGTATTTTATGATATGTGGCTACTTCTTAAAATATGAAGTGTCATCGGTTTATTAATTTAAAGTGATTTTCAACTTTTGATTATTTTTCTTTCTATTAAGTGCCAAAAATATATAATATTTTTACTTTATATATATATATATATATATTTAATTTAAAAAACTAAAGTGTCGATTTTAAAATGGTAGGTATCAAAATATACAGCAAATTGAAACCTATCCAAACTAGTGTATTTAGTCACAAAAATAATCAAATCAAATATATATATAAAAAGCTAATGAATTATTTGGTGTGGAGTACTTATTTTGGTCCATGACAAAAGCCACTTAAATTGCTAAAATTTGTGTTACCGATGAGAACAAGCACAATTAGTAGTGTACTTATCTTCTTTTTTCTTTCTTTAATTTTTTTTTCTTTTAACATAAATTAAATTAAAGGTACATATTTGAGGATATAAGAGAACCAAGATTAATCAATCGCATCTGACAAAGATAGGAAGTTCAAAATGTTCAAAACGTTCTATATTCGCCGAGGCTTACTAATCAAGATAAGCATACACGAGATACACCATCAAGATGGAACCTAAAAATCTATGCTTTTTAAGAACCCTTTCATGCCTCACATTTTCTTCTCAACAGTTCTTCAATCGCTGCAAAATCGTGTTGGTTTCGTCTGTGTTCGTAGCGATAAAATGACTCGTAAACATGACAAGAAGAAACCAGGGTTGGAAAAAGAAGAAATGAAAGCAATTTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATCAAAGAATTATCGAGAGTGTTCGGGTCGCTCGGTGCATTCTTCCCTCTCTACCGTGCAACTTGTGGCTTGTTCATTGCCGATGAGGACGGCGATGGATTTATCACTGAGCAAGAACTAGAGAAAGTGATAGATTATGCTATTAAATGTAAGTATAACATAATATAG

mRNA sequence

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGTTCTTCAATCGCTGCAAAATCGTGTTGGTTTCGTCTGTGTTCGTAGCGATAAAATGACTCGTAAACATGACAAGAAGAAACCAGGGTTGGAAAAAGAAGAAATGAAAGCAATTTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATCAAAGAATTATCGAGAGTGTTCGGGTCGCTCGGTGCATTCTTCCCTCTCTACCGTGCAACTTGTGGCTTGTTCATTGCCGATGAGGACGGCGATGGATTTATCACTGAGCAAGAACTAGAGAAAGTGATAGATTATGCTATTAAATGTAAGTATAACATAATATAG

Coding sequence (CDS)

ATGGCGTTGTCAAGATCACAACTGAAGTTGTCAAGGGATGAGATTAAGGAGATCTTACAAGACCATGATATTGATGGAGATGGCACCCTCTGCAAGAAAGAGGTGGTGCAAGCCCTGCAATCTTTAGGCACTTGGACTGCGCATCAGAAAGCTCACTATGGTGTTGCGTTTGCTGACGAAGATGTTCTTCAATCGCTGCAAAATCGTGTTGGTTTCGTCTGTGTTCGTAGCGATAAAATGACTCGTAAACATGACAAGAAGAAACCAGGGTTGGAAAAAGAAGAAATGAAAGCAATTTTTAGGGAGCATGATTTCAATGGCGACGGTCGCTTGAGCATCAAAGAATTATCGAGAGTGTTCGGGTCGCTCGGTGCATTCTTCCCTCTCTACCGTGCAACTTGTGGCTTGTTCATTGCCGATGAGGACGGCGATGGATTTATCACTGAGCAAGAACTAGAGAAAGTGATAGATTATGCTATTAAATGTAAGTATAACATAATATAG

Protein sequence

MALSRSQLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGLFIADEDGDGFITEQELEKVIDYAIKCKYNII

Homology

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BLAST of Lsi08G014660 vs. ExPASy Swiss-Prot
Match: Q8K4K1 (Centrin-4 OS=Mus musculus OX=10090 GN=Cetn4 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-07
Identity = 45/145 (31.03%), Postives = 69/145 (47.59%), Query Frame = 0


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVG 71
           + EIKE     DIDG GT+  KE+  A+++LG     ++    +A  D++   ++     
Sbjct: 26  KQEIKEAFDLFDIDGSGTIDLKELKIAMRALGFEPKKEEVKQLIAEIDKEGTGTICFE-D 85

Query: 72  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 131
           F  + S KM+ K        EKEE+   F+  D +  G +S+  + RV   LG       
Sbjct: 86  FFAIMSVKMSEKD-------EKEEILKAFKLFDDDATGSISLNNIKRVAKELGENLTEDE 145

Query: 132 ATCGLFIADEDGDGFITEQELEKVI 157
               L  AD DGDG I E+E  K++
Sbjct: 146 LQEMLDEADRDGDGEINEEEFLKMM 162

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BLAST of Lsi08G014660 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-07
Identity = 45/149 (30.20%), Postives = 71/149 (47.65%), Query Frame = 0


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQ--NRVG 73
           E+K + Q  D +GDGT+  KE+ + L+SL           G+   D+++ Q ++  +  G
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSL-----------GIYIPDKELTQMIEKIDVNG 64

Query: 74  FVCVRSDKMTR--KHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPL 133
             CV  D+     K    +   E+E+MK  F   D NGDG +++ EL  V  SLG     
Sbjct: 65  DGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 124

Query: 134 YRATCGLFI--ADEDGDGFITEQELEKVI 157
               C   I   D DGDG +  +E  +++
Sbjct: 125 TLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of Lsi08G014660 vs. ExPASy Swiss-Prot
Match: Q338P8 (Probable calcium-binding protein CML8 OS=Oryza sativa subsp. japonica OX=39947 GN=CML8 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-07
Identity = 42/145 (28.97%), Postives = 73/145 (50.34%), Query Frame = 0


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVG 71
           R EIKE     D DG GT+  KE+  A+++LG     ++ H  +A  D+D   ++ +   
Sbjct: 45  RKEIKEAFDLFDTDGSGTIDPKELNVAMRALGFEMTPEQIHQMIAEVDKDGSGTI-DFDE 104

Query: 72  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 131
           FV + +DKM  +         +EE+   F+  D + +G++S  ++ R+    G  F L  
Sbjct: 105 FVHMMTDKMGERD-------AREELNKAFKIIDKDNNGKISDVDIQRLAIETGEPFTLDE 164

Query: 132 ATCGLFIADEDGDGFITEQELEKVI 157
               +  ADE+GDG +  +E  K++
Sbjct: 165 VREMIEAADENGDGEVDHEEFLKMM 181

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BLAST of Lsi08G014660 vs. ExPASy Swiss-Prot
Match: Q12798 (Centrin-1 OS=Homo sapiens OX=9606 GN=CETN1 PE=1 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.1e-07
Identity = 40/145 (27.59%), Postives = 68/145 (46.90%), Query Frame = 0


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVG 71
           + E++E     D+DG GT+  KE+  A+++LG     ++    ++  D +    +     
Sbjct: 30  KQEVREAFDLFDVDGSGTIDAKELKVAMRALGFEPRKEEMKKMISEVDREGTGKISFN-D 89

Query: 72  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 131
           F+ V + KM+ K         KEE+   FR  D +  G++S K L RV   LG       
Sbjct: 90  FLAVMTQKMSEKD-------TKEEILKAFRLFDDDETGKISFKNLKRVANELGENLTDEE 149

Query: 132 ATCGLFIADEDGDGFITEQELEKVI 157
               +  AD DGDG + E+E  +++
Sbjct: 150 LQEMIDEADRDGDGEVNEEEFLRIM 166

[Close]

BLAST of Lsi08G014660 vs. ExPASy Swiss-Prot
Match: P41208 (Centrin-2 OS=Homo sapiens OX=9606 GN=CETN2 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 6.9e-07
Identity = 42/145 (28.97%), Postives = 70/145 (48.28%), Query Frame = 0


Query: 12  RDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVG 71
           + EI+E     D DG GT+  KE+  A+++LG     ++    ++  D++    + N   
Sbjct: 30  KQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKM-NFGD 89

Query: 72  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 131
           F+ V + KM+ K         KEE+   F+  D +  G++S K L RV   LG       
Sbjct: 90  FLTVMTQKMSEKD-------TKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEE 149

Query: 132 ATCGLFIADEDGDGFITEQELEKVI 157
               +  AD DGDG ++EQE  +++
Sbjct: 150 LQEMIDEADRDGDGEVSEQEFLRIM 166

[Close]

BLAST of Lsi08G014660 vs. ExPASy TrEMBL
Match: A0A5A7UY46 (Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00260 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.9e-32
Identity = 73/91 (80.22%), Postives = 81/91 (89.01%), Query Frame = 0


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR  +DKKK G  L+KEEMK IFREHDFNGDGRLSIKELSR FGS GAFFPLYRA  GL
Sbjct: 1   MTRPLYDKKKSGYKLQKEEMKQIFREHDFNGDGRLSIKELSRAFGSFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIKCKYNII 168
           F+AD+DGDGFI+EQELEKV+DYAIKCKYN++
Sbjct: 61  FVADDDGDGFISEQELEKVVDYAIKCKYNLL 91

[Close]

BLAST of Lsi08G014660 vs. ExPASy TrEMBL
Match: A0A0A0LKW4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358860 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 3.2e-31
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 0


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR+ +DKKK G  L+KEEMK +FREHDFNGDGRLSIKELSR FG  GAFFPLYRA  GL
Sbjct: 1   MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIKCKYNII 168
           F+AD+DGDGFI+EQEL+KV+DYAIKCKYN++
Sbjct: 61  FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Lsi08G014660 vs. ExPASy TrEMBL
Match: A0A6J1CS20 (calmodulin-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111014054 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 2.8e-27
Identity = 63/90 (70.00%), Postives = 73/90 (81.11%), Query Frame = 0


Query: 80  MTRKHDKKKPGLE--KEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGLF 139
           MT K  KKKP ++  +EE+KAIFR HD NGDG LSIKELSR FGSLGA  PLYRA CG+ 
Sbjct: 1   MTHKCVKKKPSIKLLREEVKAIFRAHDINGDGHLSIKELSRAFGSLGALLPLYRAVCGIL 60

Query: 140 IADEDGDGFITEQELEKVIDYAIKCKYNII 168
            AD DGDG IT++ELEKV+DYA+KCKY+II
Sbjct: 61  AADADGDGLITDEELEKVVDYAVKCKYDII 90

[Close]

BLAST of Lsi08G014660 vs. ExPASy TrEMBL
Match: A0A6J1CSH1 (probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC111013915 PE=4 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 1.2e-25
Identity = 70/160 (43.75%), Postives = 95/160 (59.38%), Query Frame = 0


Query: 7   QLKLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADED----- 66
           ++K SR +IKEI  +HDIDGDG L   E+++A   LG+     KAHYG+AFAD+D     
Sbjct: 70  KIKFSRSQIKEIFMEHDIDGDGCLSVSELIKAFSFLGSVIPLYKAHYGLAFADDDGDGLI 129

Query: 67  VLQSLQNRVGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFG 126
             + L   V +    SD+ T         L +E MK IF+ HD N DG L+++EL++ FG
Sbjct: 130 SEEELDKLVDYAEKGSDRKT-----SNMPLSREHMKEIFKYHDSNKDGFLNVRELTKAFG 189

Query: 127 SLGAFFPLYRATCGLFIADEDGDGFITEQELEKVIDYAIK 162
            LG+  P  +A  GL  AD + DG I E EL+K+IDYA K
Sbjct: 190 FLGSIIPFCKAHYGLVHADANNDGLINETELDKLIDYAYK 224

[Close]

BLAST of Lsi08G014660 vs. ExPASy TrEMBL
Match: A0A6J1KUW2 (calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.9e-15
Identity = 58/167 (34.73%), Postives = 91/167 (54.49%), Query Frame = 0


Query: 3   LSRSQLKLSRD-------EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGV 62
           +S + L+L RD       E +E +Q++D + D +L K+E+  A+        H +A    
Sbjct: 1   MSEASLQLHRDQAHGAEAEAQEFIQNYDKNADSSLSKEELSAAIH-------HNQAKNPG 60

Query: 63  AFADEDVLQSLQNRVGFVCVRSDKMTRKHDKKKPG---LEKEEMKAIFREHDFNGDGRLS 122
           +   E   +        V  ++ K+  K  ++K     L + ++K IF EHD +GDG LS
Sbjct: 61  SHKPEAKKKD-------VAPKAQKVPAKAAEEKGASMKLSRSQIKEIFMEHDIDGDGYLS 120

Query: 123 IKELSRVFGSLGAFFPLYRATCGLFIADEDGDGFITEQELEKVIDYA 160
           + EL++ F   G+  PLY+A  GL  AD DGDG I+E+ELEK++DYA
Sbjct: 121 VSELTKAFSFFGSIIPLYKAHYGLAYADADGDGLISEEELEKLVDYA 153

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: KAA0060893.1 (putative calcium-binding protein CML10 [Cucumis melo var. makuwa])

HSP 1 Score: 148.3 bits (373), Expect = 6.0e-32
Identity = 73/91 (80.22%), Postives = 81/91 (89.01%), Query Frame = 0


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR  +DKKK G  L+KEEMK IFREHDFNGDGRLSIKELSR FGS GAFFPLYRA  GL
Sbjct: 1   MTRPLYDKKKSGYKLQKEEMKQIFREHDFNGDGRLSIKELSRAFGSFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIKCKYNII 168
           F+AD+DGDGFI+EQELEKV+DYAIKCKYN++
Sbjct: 61  FVADDDGDGFISEQELEKVVDYAIKCKYNLL 91

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: KGN62515.1 (hypothetical protein Csa_022644 [Cucumis sativus])

HSP 1 Score: 144.8 bits (364), Expect = 6.6e-31
Identity = 70/91 (76.92%), Postives = 81/91 (89.01%), Query Frame = 0


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR+ +DKKK G  L+KEEMK +FREHDFNGDGRLSIKELSR FG  GAFFPLYRA  GL
Sbjct: 1   MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIKCKYNII 168
           F+AD+DGDGFI+EQEL+KV+DYAIKCKYN++
Sbjct: 61  FLADDDGDGFISEQELDKVVDYAIKCKYNLL 91

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: XP_004152805.1 (probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 132.9 bits (333), Expect = 2.6e-27
Identity = 66/85 (77.65%), Postives = 75/85 (88.24%), Query Frame = 0


Query: 80  MTRK-HDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGL 139
           MTR+ +DKKK G  L+KEEMK +FREHDFNGDGRLSIKELSR FG  GAFFPLYRA  GL
Sbjct: 1   MTRQLYDKKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGL 60

Query: 140 FIADEDGDGFITEQELEKVIDYAIK 162
           F+AD+DGDGFI+EQEL+KV+DYAIK
Sbjct: 61  FLADDDGDGFISEQELDKVVDYAIK 85

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: XP_022144349.1 (calmodulin-like protein 5 [Momordica charantia])

HSP 1 Score: 131.7 bits (330), Expect = 5.8e-27
Identity = 63/90 (70.00%), Postives = 73/90 (81.11%), Query Frame = 0


Query: 80  MTRKHDKKKPGLE--KEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGLF 139
           MT K  KKKP ++  +EE+KAIFR HD NGDG LSIKELSR FGSLGA  PLYRA CG+ 
Sbjct: 1   MTHKCVKKKPSIKLLREEVKAIFRAHDINGDGHLSIKELSRAFGSLGALLPLYRAVCGIL 60

Query: 140 IADEDGDGFITEQELEKVIDYAIKCKYNII 168
            AD DGDG IT++ELEKV+DYA+KCKY+II
Sbjct: 61  AADADGDGLITDEELEKVVDYAVKCKYDII 90

[Close]

BLAST of Lsi08G014660 vs. NCBI nr
Match: KAG6585659.1 (hypothetical protein SDJN03_18392, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 129.0 bits (323), Expect = 3.7e-26
Identity = 63/90 (70.00%), Postives = 73/90 (81.11%), Query Frame = 0


Query: 80  MTRKHDKKKPG--LEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRATCGLF 139
           M  K+ KK     L +EE+KAIF+EHDFNGDGRLSI+ELSR FG LGAF PLYRA  G+ 
Sbjct: 1   MASKYGKKNSSSMLPREEIKAIFKEHDFNGDGRLSIQELSRAFGMLGAFLPLYRAVRGIL 60

Query: 140 IADEDGDGFITEQELEKVIDYAIKCKYNII 168
           IAD +GDG+ITE EL+KV+DYAIKCKYNII
Sbjct: 61  IADVNGDGYITEHELDKVVDYAIKCKYNII 90

[Close]

BLAST of Lsi08G014660 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 57.0 bits (136), Expect = 1.7e-08
Identity = 45/149 (30.20%), Postives = 71/149 (47.65%), Query Frame = 0


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQ--NRVG 73
           E+K + Q  D +GDGT+  KE+ + L+SL           G+   D+++ Q ++  +  G
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSL-----------GIYIPDKELTQMIEKIDVNG 64

Query: 74  FVCVRSDKMTR--KHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPL 133
             CV  D+     K    +   E+E+MK  F   D NGDG +++ EL  V  SLG     
Sbjct: 65  DGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 124

Query: 134 YRATCGLFI--ADEDGDGFITEQELEKVI 157
               C   I   D DGDG +  +E  +++
Sbjct: 125 TLDDCKKMIKKVDVDGDGRVNYKEFRQMM 142

[Close]

BLAST of Lsi08G014660 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 54.7 bits (130), Expect = 8.4e-08
Identity = 40/142 (28.17%), Postives = 70/142 (49.30%), Query Frame = 0


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQ--NRVG 73
           ++K + Q  D +GDG + K+E+  +L++L           G+   D+D++Q +Q  +  G
Sbjct: 51  DLKRVFQMFDKNGDGRITKEELNDSLENL-----------GIFMPDKDLIQMIQKMDANG 110

Query: 74  FVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYR 133
             CV  ++    +       E+ +M+  F   D +GDG ++++EL+ V  SLG       
Sbjct: 111 DGCVDINEFESLYGSIVEEKEEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTL 170

Query: 134 ATCGLFI--ADEDGDGFITEQE 152
             C   I   DEDGDG +  +E
Sbjct: 171 ECCKEMIMQVDEDGDGRVNYKE 181

[Close]

BLAST of Lsi08G014660 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 52.8 bits (125), Expect = 3.2e-07
Identity = 43/153 (28.10%), Postives = 70/153 (45.75%), Query Frame = 0


Query: 9   KLSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLG--------TWTAHQKAHYGVAFADE 68
           ++   E+K + Q  D +GDG + K+E+  +L++LG        T   H+    G    D 
Sbjct: 60  RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDI 119

Query: 69  DVLQSLQNRVGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVF 128
           D  +SL + +         +   H+  +   E+E+MK  F   D +GDG ++++EL  V 
Sbjct: 120 DEFESLYSSI---------VDEHHNDGE--TEEEDMKDAFNVFDQDGDGFITVEELKSVM 179

Query: 129 GSLGAFFPLYRATCGLFI--ADEDGDGFITEQE 152
            SLG         C   I   D DGDG +  +E
Sbjct: 180 ASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKE 201

[Close]

BLAST of Lsi08G014660 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 50.1 bits (118), Expect = 2.1e-06
Identity = 38/149 (25.50%), Postives = 68/149 (45.64%), Query Frame = 0


Query: 10  LSRDEIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNR 69
           + R E+  + Q  D +GDG + K E+    +S+G      + +  +A  D +      + 
Sbjct: 1   MDRGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNG-DGAMDI 60

Query: 70  VGFVCVRSDKMTRKHDKKKPGLEKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPL 129
             F  +  + +  K        E+E+M+  FR  D NGDG ++ +EL  V  S+G     
Sbjct: 61  DEFGSLYQEMVEEKE-------EEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGR 120

Query: 130 YRATCGLFIA--DEDGDGFITEQELEKVI 157
               C   I+  D DGDG +  +E ++++
Sbjct: 121 TLEDCKKMISKVDVDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi08G014660 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 45.8 bits (107), Expect = 3.9e-05
Identity = 40/144 (27.78%), Postives = 64/144 (44.44%), Query Frame = 0


Query: 14  EIKEILQDHDIDGDGTLCKKEVVQALQSLGTWTAHQKAHYGVAFADEDVLQSLQNRVGFV 73
           ++K+I    D+D DG+L   E+   L+SLG   +  + H  +A  D        N  GFV
Sbjct: 7   QLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMD-------SNGNGFV 66

Query: 74  CVRSDKMTRKHDKKKPGL-EKEEMKAIFREHDFNGDGRLSIKELSRVFGSLGAFFPLYRA 133
                  T   D  +  L   E++  IF+  D +G+G +S  EL+     +G        
Sbjct: 67  EFDELVGTILPDLNEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKEL 126

Query: 134 TCGLFIADEDGDGFITEQELEKVI 157
           T  +  AD +GDG I+  E   ++
Sbjct: 127 TEMIKEADTNGDGVISFGEFASIM 143

The following BLAST results are available for this feature:

BLAST of Lsi08G014660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8K4K1	2.4e-07	31.03	Centrin-4 OS=Mus musculus OX=10090 GN=Cetn4 PE=1 SV=1	[more]
Q9LNE7	2.4e-07	30.20	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q338P8	2.4e-07	28.97	Probable calcium-binding protein CML8 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q12798	3.1e-07	27.59	Centrin-1 OS=Homo sapiens OX=9606 GN=CETN1 PE=1 SV=1	[more]
P41208	6.9e-07	28.97	Centrin-2 OS=Homo sapiens OX=9606 GN=CETN2 PE=1 SV=1	[more]

BLAST of Lsi08G014660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UY46	2.9e-32	80.22	Putative calcium-binding protein CML10 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A0A0LKW4	3.2e-31	76.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G358860 PE=4 SV=1	[more]
A0A6J1CS20	2.8e-27	70.00	calmodulin-like protein 5 OS=Momordica charantia OX=3673 GN=LOC111014054 PE=4 SV...	[more]
A0A6J1CSH1	1.2e-25	43.75	probable calcium-binding protein CML15 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1KUW2	1.9e-15	34.73	calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1	[more]

BLAST of Lsi08G014660 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0060893.1	6.0e-32	80.22	putative calcium-binding protein CML10 [Cucumis melo var. makuwa]	[more]
KGN62515.1	6.6e-31	76.92	hypothetical protein Csa_022644 [Cucumis sativus]	[more]
XP_004152805.1	2.6e-27	77.65	probable calcium-binding protein CML10 [Cucumis sativus]	[more]
XP_022144349.1	5.8e-27	70.00	calmodulin-like protein 5 [Momordica charantia]	[more]
KAG6585659.1	3.7e-26	70.00	hypothetical protein SDJN03_18392, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lsi08G014660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05990.1	1.7e-08	30.20	EF hand calcium-binding protein family	[more]
AT3G59440.1	8.4e-08	28.17	Calcium-binding EF-hand family protein	[more]
AT2G43290.1	3.2e-07	28.10	Calcium-binding EF-hand family protein	[more]
AT4G12860.1	2.1e-06	25.50	EF hand calcium-binding protein family	[more]
AT1G18530.1	3.9e-05	27.78	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 14..42 e-value: 3.5E-4 score: 29.9 coord: 95..123 e-value: 7.1E-4 score: 28.9
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 95..124 e-value: 4.0E-6 score: 26.2
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 139..155 e-value: 0.0034 score: 16.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 91..126 score: 14.150764
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 10..45 score: 13.09074
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 95..152 e-value: 4.53682E-7 score: 42.9201
None	No IPR available	GENE3D	1.10.238.10		coord: 49..161 e-value: 3.8E-11 score: 45.2
None	No IPR available	GENE3D	1.10.238.10		coord: 1..48 e-value: 2.1E-5 score: 26.1
None	No IPR available	PANTHER	PTHR10891:SF911	SUBFAMILY NOT NAMED	coord: 90..161
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 90..161
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 140..152
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 104..116
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 3..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G014660.1	Lsi08G014660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding