Lsi08G014550 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G014550
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionorigin of replication complex subunit 2
Locationchr08: 22715175 .. 22717532 (+)
RNA-Seq ExpressionLsi08G014550
SyntenyLsi08G014550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTGAAGCGTAAAAATGGCGGGAAAAATAAAGGGAGCTTTCTCTAGGGTTCGCTCCACTACAACCATTTTCCCTCCCTTCTCGAAGTAACTGTAATCGATTGGATTGCAATTTTTCGTTGCTGTTTATCGTTATCATGGAGATCGATGACTTGGACGATGAGGAGTTGGCATTTTCAAGGAACTATTTTTTGGCTAAGGAATTGGGCGGCTCCAAAAAGAAATCTTCTGGGAAACTCGCCGACATCGACGTCGTCGACGAACAGGTGCGTAACCAAATTAAACTACTTTGATCTGTTTGAGTTTAAGGAAAAACATTTAGAAGAAATTAATGATCAGGAATTGAGGGCGGCGGCGGCGAATATTGAGCCTAAGCATGAGAAGGAGATCGCGGCTCTGATATCGAGCTACAAGAGTTTGTACTCGGAATGGGTGTTTGAGCTCAGGTGTGGCATTGCCATGATAGTTGAACTGTAGTATTGTTAGATTGAAGCTCTCTGGTTGTCATTTTTTCAAATTACTTCCGGGTTTGAAATTAATTAACTTGCTATCGAAGTTCATTTTCTTTTAAGGTTATTTGTTCCATTCCTTTTGTAGATTCGCTTAATGTTTCTTTTTCCTGTGGTCCAGATGTGGTTTTGGCCTTCTAATGTATGGATTTGGGTCTAAGAAGGCTTTGATTGAAGATTTTGCTTCGACAGCGTTGACGGATTATTCTGTAATAGTTGTTAATGGCTATCTTCAATCAGTGAATATTAAACAGGTACACTTTAGCCAATGGCTATTCACATACAGACGTACTAAATTTATTTTTATGTGTCACTGAAGTCTTGCAATTTGTTGTGTAGGTTATAGTAGCCATAGCTGAAGTATTGTCGGATCAGTTGAAATCCCGACCCAAAAATGCATCAGGGAGCACACCTAATGTTCATCAGCCATTTACTTCGCGATCCATGGATGATCTGTTTACATTTTTGGATAGATCAGATGAGGAAGACAAGGATTGTTTTGTTTGTGTTGTGATACACAACATCGATGGGCCTGGGTTAAGAGATTCTGAAACGCAAGAGTATCTTGCACGAGTTGCTGCTTGTTCCCGTGTTCGAATTATTGTCTCCGTCGACCACGTGAATTCTCCTCTTTGTAAGTATATGCATTTTATTTTCATCAATTTTTATATGAATTATGATTTTCAAACATTAAACCTATGCCTCTATTGCATATTGCTATTTGTAGAGGTGCAATTTTGTTAAATTGGAAAATGTCATAGTCTTGCTTTCCATATACAGACATTGGATGTTGAGTTCAACAACATGTGGGATTAAGGAATTCAAATCTCTGGCCTCTTAGATTGAGGATATATGCCTAAACTAGTTGACCGTATGCTCAAGTTGGTAAGACCGCGGATTTTGTTGCAAGTCGAAGTATCATGCTTTGGATTCTCAAGATTTACAGTGGGAATTCAAAGAATAGAAATAAGGGTTGTTAGTGTAATCATGACTTCTATGCAAAGTTCTTTAGATAAAGGTGGATTATCGTTATAAAAATTATATTTTCATCTCTTTGCACAGAGTGTCGTGAGATAATCAAGTACTGCAACATAAATTTACTGCCAGAAGATATTGTATGAAGCATTGCAAATTGCACTATTTCATTGGTTTTTATCTCACTGAATCATGTCTTTGGTTTCTCTAGTGTGGGACAAGAAGATGGTTCACACACAATTTAACTGGTTATGGTATCATGTTCCAACATTTGCCCCTTACAAGATTGAAGGAATGTTCTTCCCTTTGATTCTTGCACATGGTGGTACTGCTCAAAGTGCCAAAACTGCTACAATAGTTTTACAGAGTTTGACACCCAATGCACAAAGTGTATTTAAAGTTCTTATAGAACATCAGCTTTCTCATCCTGATGAAGAAGGTAATTGAAGTTAATCTGATAGTATTCATTGGTTTCAAACACTTAAAATATCGAAGTAGCTTGTTGTATGAATAATGTTGATGTTATAATCTCAACTTTTCAGGTATGCCAATCAATAAACTATATGCAATTTGTCGGGAGCGCTTCCTAGTCAGCAGCCAGGTAACACTGAACTCTCATTTGACCGAATTTAAAGACCACGAGTTGGTGAAGACCAGAAGGCATTCTGATGGTCAAGATTGCTTGTATATACCTCTTACCTCCGAAGCACTTGAAAAACTATTAATGGAGTTGAGTTAAGTTTGCACATTCATTCTTTCTGTACCTGTCAACAAAGTCTGTGAATGTGTGTTAGTATGGTTGAAAGTTCAAGTTTTACCTGTTATCTCTTCGATTACTCTTAAATTTCAAGTTAGATATTTACTATTTAGATTTAA

mRNA sequence

ATTGAAGCGTAAAAATGGCGGGAAAAATAAAGGGAGCTTTCTCTAGGGTTCGCTCCACTACAACCATTTTCCCTCCCTTCTCGAAGTAACTGTAATCGATTGGATTGCAATTTTTCGTTGCTGTTTATCGTTATCATGGAGATCGATGACTTGGACGATGAGGAGTTGGCATTTTCAAGGAACTATTTTTTGGCTAAGGAATTGGGCGGCTCCAAAAAGAAATCTTCTGGGAAACTCGCCGACATCGACGTCGTCGACGAACAGGAAAAACATTTAGAAGAAATTAATGATCAGGAATTGAGGGCGGCGGCGGCGAATATTGAGCCTAAGCATGAGAAGGAGATCGCGGCTCTGATATCGAGCTACAAGAGTTTGTACTCGGAATGGGTGTTTGAGCTCAGATGTGGTTTTGGCCTTCTAATGTATGGATTTGGGTCTAAGAAGGCTTTGATTGAAGATTTTGCTTCGACAGCGTTGACGGATTATTCTGTAATAGTTGTTAATGGCTATCTTCAATCAGTGAATATTAAACAGGTTATAGTAGCCATAGCTGAAGTATTGTCGGATCAGTTGAAATCCCGACCCAAAAATGCATCAGGGAGCACACCTAATGTTCATCAGCCATTTACTTCGCGATCCATGGATGATCTGTTTACATTTTTGGATAGATCAGATGAGGAAGACAAGGATTGTTTTGTTTGTGTTGTGATACACAACATCGATGGGCCTGGGTTAAGAGATTCTGAAACGCAAGAGTATCTTGCACGAGTTGCTGCTTGTTCCCGTGTTCGAATTATTGTCTCCGTCGACCACGTGAATTCTCCTCTTTTGTGGGACAAGAAGATGGTTCACACACAATTTAACTGGTTATGGTATCATGTTCCAACATTTGCCCCTTACAAGATTGAAGGAATGTTCTTCCCTTTGATTCTTGCACATGGTGGTACTGCTCAAAGTGCCAAAACTGCTACAATAGTTTTACAGAGTTTGACACCCAATGCACAAAGTGTATTTAAAGTTCTTATAGAACATCAGCTTTCTCATCCTGATGAAGAAGGTATGCCAATCAATAAACTATATGCAATTTGTCGGGAGCGCTTCCTAGTCAGCAGCCAGGTAACACTGAACTCTCATTTGACCGAATTTAAAGACCACGAGTTGGTGAAGACCAGAAGGCATTCTGATGGTCAAGATTGCTTGTATATACCTCTTACCTCCGAAGCACTTGAAAAACTATTAATGGAGTTGAGTTAAGTTTGCACATTCATTCTTTCTGTACCTGTCAACAAAGTCTGTGAATGTGTGTTAGTATGGTTGAAAGTTCAAGTTTTACCTGTTATCTCTTCGATTACTCTTAAATTTCAAGTTAGATATTTACTATTTAGATTTAA

Coding sequence (CDS)

ATGGAGATCGATGACTTGGACGATGAGGAGTTGGCATTTTCAAGGAACTATTTTTTGGCTAAGGAATTGGGCGGCTCCAAAAAGAAATCTTCTGGGAAACTCGCCGACATCGACGTCGTCGACGAACAGGAAAAACATTTAGAAGAAATTAATGATCAGGAATTGAGGGCGGCGGCGGCGAATATTGAGCCTAAGCATGAGAAGGAGATCGCGGCTCTGATATCGAGCTACAAGAGTTTGTACTCGGAATGGGTGTTTGAGCTCAGATGTGGTTTTGGCCTTCTAATGTATGGATTTGGGTCTAAGAAGGCTTTGATTGAAGATTTTGCTTCGACAGCGTTGACGGATTATTCTGTAATAGTTGTTAATGGCTATCTTCAATCAGTGAATATTAAACAGGTTATAGTAGCCATAGCTGAAGTATTGTCGGATCAGTTGAAATCCCGACCCAAAAATGCATCAGGGAGCACACCTAATGTTCATCAGCCATTTACTTCGCGATCCATGGATGATCTGTTTACATTTTTGGATAGATCAGATGAGGAAGACAAGGATTGTTTTGTTTGTGTTGTGATACACAACATCGATGGGCCTGGGTTAAGAGATTCTGAAACGCAAGAGTATCTTGCACGAGTTGCTGCTTGTTCCCGTGTTCGAATTATTGTCTCCGTCGACCACGTGAATTCTCCTCTTTTGTGGGACAAGAAGATGGTTCACACACAATTTAACTGGTTATGGTATCATGTTCCAACATTTGCCCCTTACAAGATTGAAGGAATGTTCTTCCCTTTGATTCTTGCACATGGTGGTACTGCTCAAAGTGCCAAAACTGCTACAATAGTTTTACAGAGTTTGACACCCAATGCACAAAGTGTATTTAAAGTTCTTATAGAACATCAGCTTTCTCATCCTGATGAAGAAGGTATGCCAATCAATAAACTATATGCAATTTGTCGGGAGCGCTTCCTAGTCAGCAGCCAGGTAACACTGAACTCTCATTTGACCGAATTTAAAGACCACGAGTTGGTGAAGACCAGAAGGCATTCTGATGGTCAAGATTGCTTGTATATACCTCTTACCTCCGAAGCACTTGAAAAACTATTAATGGAGTTGAGTTAA

Protein sequence

MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAANIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVIVVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSDEEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHTQFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQLSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLTSEALEKLLMELS
Homology
[Close]
BLAST of Lsi08G014550 vs. ExPASy Swiss-Prot
Match: Q38899 (Origin of replication complex subunit 2 OS=Arabidopsis thaliana OX=3702 GN=ORC2 PE=1 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 3.5e-144
Identity = 245/371 (66.04%), Postives = 310/371 (83.56%), Query Frame = 0 

Query: 2   EIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAAN 61
           +I++++++E  FSRNYFLAKELGG+ K+S+ KL+DI +VDE          QELR  A+ 
Sbjct: 3   DIENIEEDEYGFSRNYFLAKELGGASKRSAHKLSDIHIVDE----------QELRETAST 62

Query: 62  IEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVIV 121
           IE KH KEI+ L+S YK++YS+WVFELRCGFGLLMYGFGSKKAL+EDFAS +LTDYSV+V
Sbjct: 63  IEMKHSKEISELMSDYKTMYSKWVFELRCGFGLLMYGFGSKKALVEDFASASLTDYSVVV 122

Query: 122 VNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSDE 181
           +NGYL SVN+KQV++A+AE+LS+ LK + K +SGS     + F SRSMDD+ +FL     
Sbjct: 123 INGYLPSVNLKQVLLALAELLSELLKCKRK-SSGSLSKGQETFPSRSMDDILSFLHGPQS 182

Query: 182 EDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHTQ 241
            DKDCF+CVV+HNIDGP LRD E+Q+ LAR+++CS +R++ S+DHVN+PLLWDKKMVH Q
Sbjct: 183 GDKDCFICVVVHNIDGPALRDPESQQTLARLSSCSHIRLVASIDHVNAPLLWDKKMVHKQ 242

Query: 242 FNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQL 301
           FNWLW+HVPTFAPY +EG+FFPL+LA G TAQ+AKTA IVLQSLTPN Q+VFK+L E+QL
Sbjct: 243 FNWLWHHVPTFAPYNVEGVFFPLVLAQGSTAQTAKTAAIVLQSLTPNGQNVFKILAEYQL 302

Query: 302 SHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLTS 361
           SHPDE+GMP + LY+  RERF VSSQVTLNSHLTEFKDHELVKT+R+SDGQ+CL IPLTS
Sbjct: 303 SHPDEDGMPTDDLYSASRERFFVSSQVTLNSHLTEFKDHELVKTKRNSDGQECLNIPLTS 362

Query: 362 EALEKLLMELS 373
           +A+ +LL++L+
Sbjct: 363 DAIRQLLLDLN 362

[Close]
BLAST of Lsi08G014550 vs. ExPASy Swiss-Prot
Match: B8APQ0 (Origin of replication complex subunit 2 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_10291 PE=3 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 6.2e-133
Identity = 236/370 (63.78%), Postives = 293/370 (79.19%), Query Frame = 0 

Query: 7   DDEELAFSRNYFLAKE----LGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAANI 66
           DDEE  FSR+YFLAKE     G  + +++GKL+D+++VDE          Q LRA+ A I
Sbjct: 21  DDEEAGFSRSYFLAKEKEPSSGKKRARAAGKLSDLNLVDE----------QVLRASLAEI 80

Query: 67  EPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVIVV 126
            PKHE+E+ AL  SYK  Y  W+FELRCGFGLLMYGFGSKK L+EDFAST L+D++VIVV
Sbjct: 81  PPKHEREVEALTRSYKEQYRNWLFELRCGFGLLMYGFGSKKMLLEDFASTTLSDFTVIVV 140

Query: 127 NGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSDEE 186
           NGYL S+N+KQVIV IAE+  +Q K + K  + +T +  QPF S+S+DD+ +FL+    +
Sbjct: 141 NGYLPSINLKQVIVTIAEIFWEQTKLKRKRQT-ATRSQLQPFASQSIDDIISFLNNQTSD 200

Query: 187 DKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHTQF 246
           + D  VC++IHNIDGP LRD+E+Q+YLA+V+ C +V ++ SVDHVN+PLLWDKKMVHTQF
Sbjct: 201 NGDDNVCLLIHNIDGPALRDAESQQYLAQVSCCPQVHVVASVDHVNAPLLWDKKMVHTQF 260

Query: 247 NWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQLS 306
            W WYHVPTFAPYK+EG+F+PLILA GG AQ+ KTA +VLQSLTPNAQSVF+VL E+QL+
Sbjct: 261 KWSWYHVPTFAPYKVEGVFYPLILASGGHAQTMKTALVVLQSLTPNAQSVFRVLAEYQLA 320

Query: 307 HPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLTSE 366
           H  EEGM  + LY  CRERFLVSSQVTLNSHLTEFKDH+LVK R+HSDGQDCL+IPL S+
Sbjct: 321 HEKEEGMHFSSLYTKCRERFLVSSQVTLNSHLTEFKDHDLVKIRKHSDGQDCLHIPLVSD 379

Query: 367 ALEKLLMELS 373
           ALEKLL EL+
Sbjct: 381 ALEKLLQELT 379

[Close]
BLAST of Lsi08G014550 vs. ExPASy Swiss-Prot
Match: Q10QS7 (Origin of replication complex subunit 2 OS=Oryza sativa subsp. japonica OX=39947 GN=ORC2 PE=2 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 6.2e-133
Identity = 236/370 (63.78%), Postives = 293/370 (79.19%), Query Frame = 0 

Query: 7   DDEELAFSRNYFLAKE----LGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAANI 66
           DDEE  FSR+YFLAKE     G  + +++GKL+D+++VDE          Q LRA+ A I
Sbjct: 21  DDEEAGFSRSYFLAKEKEPSSGKKRARAAGKLSDLNLVDE----------QVLRASLAEI 80

Query: 67  EPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVIVV 126
            PKHE+E+ AL  SYK  Y  W+FELRCGFGLLMYGFGSKK L+EDFAST L+D++VIVV
Sbjct: 81  PPKHEREVEALTRSYKEQYRNWLFELRCGFGLLMYGFGSKKMLLEDFASTTLSDFTVIVV 140

Query: 127 NGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSDEE 186
           NGYL S+N+KQVIV IAE+  +Q K + K  + +T +  QPF S+S+DD+ +FL+    +
Sbjct: 141 NGYLPSINLKQVIVTIAEIFWEQTKLKRKRQT-ATRSQLQPFASQSIDDIISFLNNQTSD 200

Query: 187 DKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHTQF 246
           + D  VC++IHNIDGP LRD+E+Q+YLA+V+ C +V ++ SVDHVN+PLLWDKKMVHTQF
Sbjct: 201 NGDDNVCLLIHNIDGPALRDAESQQYLAQVSCCPQVHVVASVDHVNAPLLWDKKMVHTQF 260

Query: 247 NWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQLS 306
            W WYHVPTFAPYK+EG+F+PLILA GG AQ+ KTA +VLQSLTPNAQSVF+VL E+QL+
Sbjct: 261 KWSWYHVPTFAPYKVEGVFYPLILASGGHAQTMKTALVVLQSLTPNAQSVFRVLAEYQLA 320

Query: 307 HPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLTSE 366
           H  EEGM  + LY  CRERFLVSSQVTLNSHLTEFKDH+LVK R+HSDGQDCL+IPL S+
Sbjct: 321 HEKEEGMHFSSLYTKCRERFLVSSQVTLNSHLTEFKDHDLVKIRKHSDGQDCLHIPLVSD 379

Query: 367 ALEKLLMELS 373
           ALEKLL EL+
Sbjct: 381 ALEKLLQELT 379

[Close]
BLAST of Lsi08G014550 vs. ExPASy Swiss-Prot
Match: Q13416 (Origin recognition complex subunit 2 OS=Homo sapiens OX=9606 GN=ORC2 PE=1 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-50
Identity = 110/342 (32.16%), Postives = 191/342 (55.85%), Query Frame = 0 

Query: 30  SSGKLADIDVVDEQEKHLEEINDQELRAAAANIEPKHEKEIAALISSYKSLYSEWVFELR 89
           SS K+   D   ++ K   +++ Q LR   + + P    E+  L   Y+ L+ +W+ +L 
Sbjct: 248 SSSKVLTSDRTLQKLKR-AKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHKWMLQLH 307

Query: 90  CGFGLLMYGFGSKKALIEDFASTALTDYSVIVVNGYLQSVNIKQVIVAIAEVLSDQLKSR 149
            GF +++YG GSK+ L+E F +T L D   +V+NG+   +++K V+ +I E + D +   
Sbjct: 308 LGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVLDHMG-- 367

Query: 150 PKNASGSTPNVHQPFTSRSMDDLFTFLDRSDEEDKDCFVCVVIHNIDGPGLRDSETQEYL 209
                          T RS+ D   ++    +ED    + ++IHN+D   LR  ++Q+ +
Sbjct: 368 ---------------TFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQII 427

Query: 210 ARVAACSRVRIIVSVDHVNSPLLWDKKMVHTQFNWLWYHVPTFAPYKIEGMFFPLILAHG 269
            ++++   + +I S+DH+N+PL+WD     + FNWLWY   T++PY  E  +   +L   
Sbjct: 428 GQLSSLHNIYLIASIDHLNAPLMWDHAK-QSLFNWLWYETTTYSPYTEETSYENSLLVKQ 487

Query: 270 GTAQSAKTATIVLQSLTPNAQSVFKVLIEHQLSHPDEE---GMPINKLYAICRERFLVSS 329
             +    + T VL+SLTPNA+ +F++LI++QL + D     G+     Y  CRE FLV+S
Sbjct: 488 SGSLPLSSLTHVLRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNS 547

Query: 330 QVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLTSEALEKLL 369
            +TL + LTEF+DH+L++T++ +DG + L IP+ +  L   L
Sbjct: 548 DLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIPVDNGTLTDFL 570

[Close]
BLAST of Lsi08G014550 vs. ExPASy Swiss-Prot
Match: Q75PQ8 (Origin recognition complex subunit 2 OS=Rattus norvegicus OX=10116 GN=Orc2 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.1e-47
Identity = 103/329 (31.31%), Postives = 181/329 (55.02%), Query Frame = 0 

Query: 43  QEKHLEEINDQELRAAAANIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSK 102
           Q+     ++ + LR   +   P    EI  L   ++ L+ +W+ +LR GF +++YG GSK
Sbjct: 258 QKLRRARVDQKTLRNLLSKFVPSFSTEIGQLNQQHERLFDKWILQLRLGFNIVLYGLGSK 317

Query: 103 KALIEDFASTALTDYSVIVVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQ 162
           + L+E F +T L D   +V+NG+   +++K ++ +I E +   +                
Sbjct: 318 RDLLEKFRTTMLQDSIHVVINGFFPGISVKSILNSITEDVLSHMG--------------- 377

Query: 163 PFTSRSMDDLFTFLDRSDEEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIV 222
             T +S+ D   ++    +ED    + ++IHN+D   LR   +Q+ L ++++   V +I 
Sbjct: 378 --TFQSVLDQRDWIMNRFKEDSSLELFLLIHNLDSQMLRGDNSQQILGQLSSLRNVYLIA 437

Query: 223 SVDHVNSPLLWDKKMVHTQFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVL 282
           S+DH+N+PL+WD     + +NWLWY   T++PY  E  +   +L     +    +   VL
Sbjct: 438 SIDHLNAPLMWDHAK-QSLYNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLIHVL 497

Query: 283 QSLTPNAQSVFKVLIEHQLSH---PDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKD 342
           +SLTPNA+ +F++L+++QL +   P   G+     Y  CRE FLV+S +TL + LTEF+D
Sbjct: 498 RSLTPNARGIFRLLMKYQLDNQDCPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFRD 557

Query: 343 HELVKTRRHSDGQDCLYIPLTSEALEKLL 369
           H+L++T++ +DG + L IP+ S  L   L
Sbjct: 558 HKLIRTKKGTDGVEYLLIPVDSGTLADFL 568

[Close]
BLAST of Lsi08G014550 vs. ExPASy TrEMBL
Match: A0A6J1CSK4 (origin of replication complex subunit 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013922 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.3e-186
Identity = 330/371 (88.95%), Postives = 349/371 (94.07%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDD E AFSRNYFLAKELGGS KKS+ KL+DIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDGEFAFSRNYFLAKELGGSSKKSARKLSDIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
           N++PKHEKEIAALISSYKSLYS+WVFELRCGFGLLMYGFGSKKALIEDFAST+LTDYSVI
Sbjct: 61  NVQPKHEKEIAALISSYKSLYSKWVFELRCGFGLLMYGFGSKKALIEDFASTSLTDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           VVNGYLQS+NIKQV+VAIAE+LSDQLKSR +NASGSTP +HQPFTSRSMDD+F+FL+ S+
Sbjct: 121 VVNGYLQSINIKQVVVAIAEILSDQLKSRLRNASGSTPKIHQPFTSRSMDDIFSFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVA+CS VRII S+DHVN+PLLWDKKM+HT
Sbjct: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVASCSYVRIIASIDHVNAPLLWDKKMIHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPIN LY ICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPINSLYTICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMEL 372
           SEALEKLLMEL
Sbjct: 361 SEALEKLLMEL 361

[Close]
BLAST of Lsi08G014550 vs. ExPASy TrEMBL
Match: A0A0A0LRD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359990 PE=3 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 8.6e-186
Identity = 336/372 (90.32%), Postives = 348/372 (93.55%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           ME+DDLDDEE AFSRNYFLAKELGGSKKKSSGKLADIDVVDE          QELRAAAA
Sbjct: 1   MEVDDLDDEEFAFSRNYFLAKELGGSKKKSSGKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
           N EPKHE+EIAALISSYKS Y++WVFELRCGFGLLMYGFGSKK+LIEDFASTAL DYSVI
Sbjct: 61  NTEPKHEREIAALISSYKSSYAKWVFELRCGFGLLMYGFGSKKSLIEDFASTALMDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           VVNGYLQSVNIKQVI+AIAE LSDQLKSRPKNASGS  NVHQ F+SRSMDDLF FL+ S+
Sbjct: 121 VVNGYLQSVNIKQVIIAIAEELSDQLKSRPKNASGS--NVHQTFSSRSMDDLFVFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACS VRII SVDHVN+PLLWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSHVRIIASVDHVNAPLLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPI+KLYAICRERFLVSSQ+TLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPIDKLYAICRERFLVSSQITLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMELS 373
           SEALEKLL ELS
Sbjct: 361 SEALEKLLTELS 360

[Close]
BLAST of Lsi08G014550 vs. ExPASy TrEMBL
Match: A0A6J1GGS0 (origin of replication complex subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC111454083 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.3e-185
Identity = 336/371 (90.57%), Postives = 346/371 (93.26%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDDEELAFSRNYFLAKELGGSKKKSS KLADIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSRKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
            IEPKHEKEIAALI+SYKSLYS+W FELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI
Sbjct: 61  KIEPKHEKEIAALIASYKSLYSKWEFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           V+NGYLQSVNIKQVIVAIAEVLSDQLKSR +NASGST  VH PFTSRSMDDLF+FL+ S+
Sbjct: 121 VINGYLQSVNIKQVIVAIAEVLSDQLKSRVRNASGSTSKVHHPFTSRSMDDLFSFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDG GLRDSETQEYLARVAACS VRII S+DHVN+PLLWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGAGLRDSETQEYLARVAACSHVRIIASIDHVNAPLLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFA YK+EGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFASYKVEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPIN LYAI RERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPINNLYAISRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMEL 372
           SEALEKLLMEL
Sbjct: 361 SEALEKLLMEL 361

[Close]
BLAST of Lsi08G014550 vs. ExPASy TrEMBL
Match: A0A6J1KMJ5 (origin of replication complex subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111495969 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 2.1e-184
Identity = 334/371 (90.03%), Postives = 346/371 (93.26%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDDEELAFSRNYFLAKELGGS KKSS KLADIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDEELAFSRNYFLAKELGGSTKKSSRKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
            IEPKHEKEIAALI+SYKSLYS+W FELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI
Sbjct: 61  KIEPKHEKEIAALIASYKSLYSKWEFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           V+NGYLQSVNIKQVIVAIAEVLSDQLKSR +NASGST  VHQPFTSRSMDDLF+FL+ S+
Sbjct: 121 VINGYLQSVNIKQVIVAIAEVLSDQLKSRVRNASGSTSKVHQPFTSRSMDDLFSFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDG GLRDSETQ+YLARVAACS VRII S+DHVN+PLLWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGVGLRDSETQDYLARVAACSHVRIIASIDHVNAPLLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFA YK+EGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFASYKVEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPIN LYAI RERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPINNLYAISRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMEL 372
           SEALEKL+MEL
Sbjct: 361 SEALEKLIMEL 361

[Close]
BLAST of Lsi08G014550 vs. ExPASy TrEMBL
Match: A0A5A7V592 (Origin of replication complex subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00110 PE=3 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 1.0e-183
Identity = 335/372 (90.05%), Postives = 343/372 (92.20%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDDEE AFSRNYFLAKELGGSKKKSSGKLADIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDEEFAFSRNYFLAKELGGSKKKSSGKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
           NIEPKHE EIAALISSYKS YS+WVFELRCGFGLLMYGFGSKK+LIEDFAS AL DYSVI
Sbjct: 61  NIEPKHESEIAALISSYKSSYSKWVFELRCGFGLLMYGFGSKKSLIEDFASRALVDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           VVNGYLQSVNIKQVIVAIAE LSDQLKSRPKNASGS  N HQ F+SRSMDDLF FL+ S+
Sbjct: 121 VVNGYLQSVNIKQVIVAIAEELSDQLKSRPKNASGS--NAHQTFSSRSMDDLFVFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACS VRII SVDHVN+PLLWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSHVRIIASVDHVNAPLLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFAPYKIE MFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFAPYKIEAMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPI+KLYA CRERFLVSSQ+TLNSHLTEFKDHELVK RRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPIDKLYATCRERFLVSSQITLNSHLTEFKDHELVKIRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMELS 373
           SEALEKLL ELS
Sbjct: 361 SEALEKLLTELS 360

[Close]
BLAST of Lsi08G014550 vs. NCBI nr
Match: XP_038885694.1 (origin of replication complex subunit 2 [Benincasa hispida])

HSP 1 Score: 685.6 bits (1768), Expect = 2.3e-193
Identity = 350/372 (94.09%), Postives = 354/372 (95.16%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDDEELAF RNYFLAKELGGSKKKSSGKLADIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDEELAFQRNYFLAKELGGSKKKSSGKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
           NIEP HEKEIAALISSYKSLYS+WVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI
Sbjct: 61  NIEPTHEKEIAALISSYKSLYSKWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPK+ASGSTPNVHQPFTSRSMDDLFTFL+ S+
Sbjct: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKSASGSTPNVHQPFTSRSMDDLFTFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACS VRII SVDHVNSPLLWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSHVRIIASVDHVNSPLLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVK RRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKIRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMELS 373
           SEALEKL MELS
Sbjct: 361 SEALEKLSMELS 362

[Close]
BLAST of Lsi08G014550 vs. NCBI nr
Match: XP_022144163.1 (origin of replication complex subunit 2 isoform X1 [Momordica charantia])

HSP 1 Score: 661.4 bits (1705), Expect = 4.7e-186
Identity = 330/371 (88.95%), Postives = 349/371 (94.07%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDD E AFSRNYFLAKELGGS KKS+ KL+DIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDGEFAFSRNYFLAKELGGSSKKSARKLSDIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
           N++PKHEKEIAALISSYKSLYS+WVFELRCGFGLLMYGFGSKKALIEDFAST+LTDYSVI
Sbjct: 61  NVQPKHEKEIAALISSYKSLYSKWVFELRCGFGLLMYGFGSKKALIEDFASTSLTDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           VVNGYLQS+NIKQV+VAIAE+LSDQLKSR +NASGSTP +HQPFTSRSMDD+F+FL+ S+
Sbjct: 121 VVNGYLQSINIKQVVVAIAEILSDQLKSRLRNASGSTPKIHQPFTSRSMDDIFSFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVA+CS VRII S+DHVN+PLLWDKKM+HT
Sbjct: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVASCSYVRIIASIDHVNAPLLWDKKMIHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPIN LY ICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPINSLYTICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMEL 372
           SEALEKLLMEL
Sbjct: 361 SEALEKLLMEL 361

[Close]
BLAST of Lsi08G014550 vs. NCBI nr
Match: XP_004152733.1 (origin of replication complex subunit 2 [Cucumis sativus] >KGN62531.1 hypothetical protein Csa_022538 [Cucumis sativus])

HSP 1 Score: 659.4 bits (1700), Expect = 1.8e-185
Identity = 336/372 (90.32%), Postives = 348/372 (93.55%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           ME+DDLDDEE AFSRNYFLAKELGGSKKKSSGKLADIDVVDE          QELRAAAA
Sbjct: 1   MEVDDLDDEEFAFSRNYFLAKELGGSKKKSSGKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
           N EPKHE+EIAALISSYKS Y++WVFELRCGFGLLMYGFGSKK+LIEDFASTAL DYSVI
Sbjct: 61  NTEPKHEREIAALISSYKSSYAKWVFELRCGFGLLMYGFGSKKSLIEDFASTALMDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           VVNGYLQSVNIKQVI+AIAE LSDQLKSRPKNASGS  NVHQ F+SRSMDDLF FL+ S+
Sbjct: 121 VVNGYLQSVNIKQVIIAIAEELSDQLKSRPKNASGS--NVHQTFSSRSMDDLFVFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACS VRII SVDHVN+PLLWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSHVRIIASVDHVNAPLLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPI+KLYAICRERFLVSSQ+TLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPIDKLYAICRERFLVSSQITLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMELS 373
           SEALEKLL ELS
Sbjct: 361 SEALEKLLTELS 360

[Close]
BLAST of Lsi08G014550 vs. NCBI nr
Match: XP_022951151.1 (origin of replication complex subunit 2 [Cucurbita moschata])

HSP 1 Score: 657.5 bits (1695), Expect = 6.7e-185
Identity = 336/371 (90.57%), Postives = 346/371 (93.26%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDDEELAFSRNYFLAKELGGSKKKSS KLADIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSRKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
            IEPKHEKEIAALI+SYKSLYS+W FELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI
Sbjct: 61  KIEPKHEKEIAALIASYKSLYSKWEFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           V+NGYLQSVNIKQVIVAIAEVLSDQLKSR +NASGST  VH PFTSRSMDDLF+FL+ S+
Sbjct: 121 VINGYLQSVNIKQVIVAIAEVLSDQLKSRVRNASGSTSKVHHPFTSRSMDDLFSFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDG GLRDSETQEYLARVAACS VRII S+DHVN+PLLWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGAGLRDSETQEYLARVAACSHVRIIASIDHVNAPLLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFA YK+EGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFASYKVEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPIN LYAI RERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPINNLYAISRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMEL 372
           SEALEKLLMEL
Sbjct: 361 SEALEKLLMEL 361

[Close]
BLAST of Lsi08G014550 vs. NCBI nr
Match: XP_023537716.1 (origin of replication complex subunit 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 335/371 (90.30%), Postives = 346/371 (93.26%), Query Frame = 0 

Query: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAA 60
           MEIDDLDDEELAFSRNYFLAKELGGSKKKSS KLADIDVVDE          QELRAAAA
Sbjct: 1   MEIDDLDDEELAFSRNYFLAKELGGSKKKSSRKLADIDVVDE----------QELRAAAA 60

Query: 61  NIEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVI 120
            IEPKHEKEIAALI+SYKSLYS+W FELRCGFGLLM+GFGSKKALIEDFASTALTDYSVI
Sbjct: 61  KIEPKHEKEIAALIASYKSLYSKWEFELRCGFGLLMFGFGSKKALIEDFASTALTDYSVI 120

Query: 121 VVNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSD 180
           V+NGYLQSVNIKQVIVAIAEVLSDQLKSR +NASGST  VHQPFTSRSMDDLF+FL+ S+
Sbjct: 121 VINGYLQSVNIKQVIVAIAEVLSDQLKSRVRNASGSTSKVHQPFTSRSMDDLFSFLNGSN 180

Query: 181 EEDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHT 240
           EEDKDCFVCVVIHNIDG GLRDSETQEYLARVAACS VRII S+DHVN+P LWDKKMVHT
Sbjct: 181 EEDKDCFVCVVIHNIDGAGLRDSETQEYLARVAACSHVRIIASIDHVNAPFLWDKKMVHT 240

Query: 241 QFNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300
           QFNWLWYHVPTFA YK+EGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ
Sbjct: 241 QFNWLWYHVPTFASYKVEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQ 300

Query: 301 LSHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLT 360
           LSHPDEEGMPIN LYAI RERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPL 
Sbjct: 301 LSHPDEEGMPINNLYAISRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLP 360

Query: 361 SEALEKLLMEL 372
           SEALEKLLMEL
Sbjct: 361 SEALEKLLMEL 361

[Close]
BLAST of Lsi08G014550 vs. TAIR 10
Match: AT2G37560.1 (origin recognition complex second largest subunit 2 )

HSP 1 Score: 512.7 bits (1319), Expect = 2.5e-145
Identity = 245/371 (66.04%), Postives = 310/371 (83.56%), Query Frame = 0 

Query: 2   EIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAAN 61
           +I++++++E  FSRNYFLAKELGG+ K+S+ KL+DI +VDE          QELR  A+ 
Sbjct: 3   DIENIEEDEYGFSRNYFLAKELGGASKRSAHKLSDIHIVDE----------QELRETAST 62

Query: 62  IEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVIV 121
           IE KH KEI+ L+S YK++YS+WVFELRCGFGLLMYGFGSKKAL+EDFAS +LTDYSV+V
Sbjct: 63  IEMKHSKEISELMSDYKTMYSKWVFELRCGFGLLMYGFGSKKALVEDFASASLTDYSVVV 122

Query: 122 VNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSDE 181
           +NGYL SVN+KQV++A+AE+LS+ LK + K +SGS     + F SRSMDD+ +FL     
Sbjct: 123 INGYLPSVNLKQVLLALAELLSELLKCKRK-SSGSLSKGQETFPSRSMDDILSFLHGPQS 182

Query: 182 EDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHTQ 241
            DKDCF+CVV+HNIDGP LRD E+Q+ LAR+++CS +R++ S+DHVN+PLLWDKKMVH Q
Sbjct: 183 GDKDCFICVVVHNIDGPALRDPESQQTLARLSSCSHIRLVASIDHVNAPLLWDKKMVHKQ 242

Query: 242 FNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQL 301
           FNWLW+HVPTFAPY +EG+FFPL+LA G TAQ+AKTA IVLQSLTPN Q+VFK+L E+QL
Sbjct: 243 FNWLWHHVPTFAPYNVEGVFFPLVLAQGSTAQTAKTAAIVLQSLTPNGQNVFKILAEYQL 302

Query: 302 SHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLTS 361
           SHPDE+GMP + LY+  RERF VSSQVTLNSHLTEFKDHELVKT+R+SDGQ+CL IPLTS
Sbjct: 303 SHPDEDGMPTDDLYSASRERFFVSSQVTLNSHLTEFKDHELVKTKRNSDGQECLNIPLTS 362

Query: 362 EALEKLLMELS 373
           +A+ +LL++L+
Sbjct: 363 DAIRQLLLDLN 362

[Close]
BLAST of Lsi08G014550 vs. TAIR 10
Match: AT2G37560.2 (origin recognition complex second largest subunit 2 )

HSP 1 Score: 490.0 bits (1260), Expect = 1.7e-138
Identity = 236/371 (63.61%), Postives = 295/371 (79.51%), Query Frame = 0 

Query: 2   EIDDLDDEELAFSRNYFLAKELGGSKKKSSGKLADIDVVDEQEKHLEEINDQELRAAAAN 61
           +I++++++E  FSRNYFLAKELGG+ K+S+ KL+DI +VDE          QELR  A+ 
Sbjct: 3   DIENIEEDEYGFSRNYFLAKELGGASKRSAHKLSDIHIVDE----------QELRETAST 62

Query: 62  IEPKHEKEIAALISSYKSLYSEWVFELRCGFGLLMYGFGSKKALIEDFASTALTDYSVIV 121
           IE KH KEI+ L+S YK++YS+WVFELRCGFGLLMYGFGSKKAL+EDFAS +LTDYSV+V
Sbjct: 63  IEMKHSKEISELMSDYKTMYSKWVFELRCGFGLLMYGFGSKKALVEDFASASLTDYSVVV 122

Query: 122 VNGYLQSVNIKQVIVAIAEVLSDQLKSRPKNASGSTPNVHQPFTSRSMDDLFTFLDRSDE 181
           +NGYL SVN+KQ                   +SGS     + F SRSMDD+ +FL     
Sbjct: 123 INGYLPSVNLKQ-------------------SSGSLSKGQETFPSRSMDDILSFLHGPQS 182

Query: 182 EDKDCFVCVVIHNIDGPGLRDSETQEYLARVAACSRVRIIVSVDHVNSPLLWDKKMVHTQ 241
            DKDCF+CVV+HNIDGP LRD E+Q+ LAR+++CS +R++ S+DHVN+PLLWDKKMVH Q
Sbjct: 183 GDKDCFICVVVHNIDGPALRDPESQQTLARLSSCSHIRLVASIDHVNAPLLWDKKMVHKQ 242

Query: 242 FNWLWYHVPTFAPYKIEGMFFPLILAHGGTAQSAKTATIVLQSLTPNAQSVFKVLIEHQL 301
           FNWLW+HVPTFAPY +EG+FFPL+LA G TAQ+AKTA IVLQSLTPN Q+VFK+L E+QL
Sbjct: 243 FNWLWHHVPTFAPYNVEGVFFPLVLAQGSTAQTAKTAAIVLQSLTPNGQNVFKILAEYQL 302

Query: 302 SHPDEEGMPINKLYAICRERFLVSSQVTLNSHLTEFKDHELVKTRRHSDGQDCLYIPLTS 361
           SHPDE+GMP + LY+  RERF VSSQVTLNSHLTEFKDHELVKT+R+SDGQ+CL IPLTS
Sbjct: 303 SHPDEDGMPTDDLYSASRERFFVSSQVTLNSHLTEFKDHELVKTKRNSDGQECLNIPLTS 344

Query: 362 EALEKLLMELS 373
           +A+ +LL++L+
Sbjct: 363 DAIRQLLLDLN 344

The following BLAST results are available for this feature:
BLAST of Lsi08G014550 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q388993.5e-14466.04Origin of replication complex subunit 2 OS=Arabidopsis thaliana OX=3702 GN=ORC2 ... [more]
B8APQ06.2e-13363.78Origin of replication complex subunit 2 OS=Oryza sativa subsp. indica OX=39946 G... [more]
Q10QS76.2e-13363.78Origin of replication complex subunit 2 OS=Oryza sativa subsp. japonica OX=39947... [more]
Q134161.0e-5032.16Origin recognition complex subunit 2 OS=Homo sapiens OX=9606 GN=ORC2 PE=1 SV=2[more]
Q75PQ81.1e-4731.31Origin recognition complex subunit 2 OS=Rattus norvegicus OX=10116 GN=Orc2 PE=2 ... [more]
BLAST of Lsi08G014550 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CSK42.3e-18688.95origin of replication complex subunit 2 isoform X1 OS=Momordica charantia OX=367... [more]
A0A0A0LRD08.6e-18690.32Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G359990 PE=3 SV=1[more]
A0A6J1GGS03.3e-18590.57origin of replication complex subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KMJ52.1e-18490.03origin of replication complex subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111495... [more]
A0A5A7V5921.0e-18390.05Origin of replication complex subunit 2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
BLAST of Lsi08G014550 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038885694.12.3e-19394.09origin of replication complex subunit 2 [Benincasa hispida][more]
XP_022144163.14.7e-18688.95origin of replication complex subunit 2 isoform X1 [Momordica charantia][more]
XP_004152733.11.8e-18590.32origin of replication complex subunit 2 [Cucumis sativus] >KGN62531.1 hypothetic... [more]
XP_022951151.16.7e-18590.57origin of replication complex subunit 2 [Cucurbita moschata][more]
XP_023537716.11.5e-18490.30origin of replication complex subunit 2-like [Cucurbita pepo subsp. pepo][more]
BLAST of Lsi08G014550 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G37560.12.5e-14566.04origin recognition complex second largest subunit 2 [more]
AT2G37560.21.7e-13863.61origin recognition complex second largest subunit 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007220Origin recognition complex, subunit 2PFAMPF04084ORC2coord: 46..362
e-value: 3.3E-96
score: 322.1
IPR007220Origin recognition complex, subunit 2PANTHERPTHR14052ORIGIN RECOGNITION COMPLEX SUBUNIT 2coord: 15..371

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G014550.1Lsi08G014550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006260 DNA replication
cellular_component GO:0005664 nuclear origin of replication recognition complex
cellular_component GO:0005634 nucleus
cellular_component GO:0000808 origin recognition complex
molecular_function GO:0003688 DNA replication origin binding