Lsi08G014340 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G014340
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.5-like
Locationchr08: 22566846 .. 22570745 (-)
RNA-Seq ExpressionLsi08G014340
SyntenyLsi08G014340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTTGTGTCGATGGCGCTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCGGTGACCACTCTCACCAATTTCATGGGAACCACGTTTCTTCTCACACTCGTCGGCGGTTTCGTCTCCGAAACTTATCTCTCAAGATTCAAGACTTGCGTCCTCTTCGCTTCCTTTGAGTTCATGGTTGCTCTCACCACTTTCTTTCTCTCTCTACCCTTCATTTCTTACACACAACATCAGTAATGCAAATTCTTAGAATTTAAAATACCCATTTGAATTTGATGGACTCTAATATCATATTAGATATTCTATTTCGAATTAATCAATAATACATGAGATGAGTAGCATCTCTATCTTATTAAAATTATGATGTTCCTTCCTTCTTTTCAATGTGGGATAATTCGTCAAAATGACCATAGTTTAATTAACATATAAATAATATTAAATGTCGTATTTGTTCAACATGACTTAAATTGAATTGATGGATAAGATTAGTACATGTGGAGTCAATTTTTTTTTATAGCAAAGTTAAAAGTGTCTTAAATAGGGGAAAGTTTCCTATGTTTTTTTTATGATAATATGCACAATTTTTCATACTATTTGTCACATTAATGGACTTTTAAAAATAAGGTAGGATTCGTTAAAATTAATGTGTAGTTCATAAATACTTATAAAGATAAAATTTAGGGAAATTATTGTGTATAGAAAAAAAAACAAAACTAATTACACATATAGAAAAAAGTGCACCTCATGCTTTTATATTTTCCACTTTTTTTTCTATATATGTAATTAGTTTTGTTTTTTTTTTTCTATTTGTGAAAAGACCCCATAAATTTATAGTGTGAATAAAGAGAATAAATAAATTTATAGCATAAAAATTTGTGTTGAAAAAATAATAATAATTACTAAAATAATTAGGAGAATTTTTAAAAATATAAAAATAAACAAAACTATTTATACAAAATAACAAAAATTTTAAATAGTTATTATTGACACGGATAAAAGTCTATCACAGTATATATGTAATAGAAATGATACAAGTTTATAAAAAATATATATATATAGTTTTTCTATAAATAGTTTGACATTTATTTTATGAAAATTTCTCAAATAATTATTATCTCGTCTTTTTCGGTAGGGATATTGTCTGCTAACAGCCCAGGCACATTTCCCGCACCTAAGGCCGGCACCGTGCAAAGGAGCGGCGGCGGGCAGCAAGTGCGAGGCGGCGGACAGGGGTCAGGAGGCGATTCTTTACGTGGGAATATACCTGATAGCGCTGGGAGCGAGTGGCGTGAAAGTGGCGCTGCCAGCATTGGGGGCGGATCAATTCGACGACAGAGATCCAGTGGAGGCGGCGAAGCTGTCGAGCTTTTTCAACTGGTTCCTGTTCAGTCTGACGATAGGATCGATGGTGGGAGTGACATTGATAGCGTGGATAAACACAGCGGTGGGTTGGGACTTGGCGTTTGTGGTTTGTAGCATTGCACTTCTTGCTGCCATTGTTGTTGTGCTTGCAGGCAAGTCGTTTTATCGCAATAATGTGCCTATTGGAAGCCCCGTTCTCAGACTTCTTCAGGTATACAAATATCTTTTATCTTCTTTACTTTCCTCCATTTCGATTTTCCAATATCGCTTTTCTAAAGCACACCAACAATTCAAAATCTGCACGTTGTTTAATAATCATATGAGATATAAATTTAAGATAATTTTTTTCTCGCGATAGTTACTCATATTGTAGTGACCTTGTTGAATAATTTTTCATTTGAAGATGAGTGAATATTACTAAAAAAAAAAGTAGAAAATATATAATTTTAGTCCTAGGTTTAGAGCCTTGTTTTCATTTGGTTACTAGGTTCAAAATGTTATACTTTGACCCTTGATTTTGGGTTTAATTTTTATTTTGTCCTTAAGATTCAAAATTAAGCAATACTTAGGTGCAACATAAGTTGTACCTAAGTTTTATACTCCCTTTCCTCTATCCACAAAAAATATATTTTTTAATCATTTTTTAATTGACATATGTCAATTTTTAATTGGGCGTAGCAAAGTTGTACGGCAGCCAAGTATTTTCTTTCAAAATATTACATTTTACCCTTAGTTTTAGAGTTTTGTTTTCCATTTGATGCTAGATTTCAAAATATCACATTTTGACCTTTGAGTTTTGAATTTAATTTTCATTTAGTCTATAAATTTAAAAATTACATTTTTACCCTCGCATTTTGAAGTTTTATTTCTATTGGGTCCTTAAGTTTTTAGATTTACATATTTACCCTTGATTTTTCTCTAAATACTCATTTTTGTCCTTGTTAACGTCTAATGATTAAATTAAAAGAATTGAATTAGTTTTCTTTAATTTTCATCACTATTAAAAATAATTATAAATTTTTACATCATAATTCTTTAAATAGATTAACTGAAAATTAACGCCATGAGACCAAAAGTAGGTTGCTAAAATTCATTGGATAAGAATATAAAATACTAAAACATAGAGACCAAATGAGAAAATTAAACTCAAAACTCTAAAGTAAAAATGTAACATTTAAAAACCTAGTGACCAACAAAACTAAAAACTCAAAAATAAAGAGCATCGCTATGATTATATTTTTTTTCTTTGAAAAATTATTCGATGAGGCAACATGCTACAATGTGGTTAAAAATTTTACTGCAAAATATTTTTAAATTTGTATCAAGTGGTTATTGAGAACACCTAGATTTTCAAACCCACATTGGACGGATTAAAAAAAACGTTTCACCGTGTTTTTTCCTCATAAGTAGTCTCCTTGAATTCATCAATCATGATGCAACGAACGGTTGTTCAATCCATTTTATTCTCGAAGTATAGGTCTTTGTAGCTTCAATCCGAAACAGGAAACTTCCACTTCCAGAGAATGTTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAATCCTCGAGAAAACTGATCAATTCAGGTATGATATATACCTTTTAATGATCAAAAAACAACCTAAGCTTTGGAATTCAATTGGTTGGTTGAAGGCATGAAAATTTTGAAATTCTGTAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAAGTGCATCAATGTCAGCAAATCAGCTAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAAGAAACAAAGATTCTAATCAGAATGCTCCCAATTATACTAAGCACCATCTTCATGGCCACCTGCATGGCTCAACTCCAAACATTCTCCATTCAACAAAGCATAACCATGGATTGCCATTTCCTGGGATTCAAAATTCCAGGCCCCTCTGTCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTATTTTCGTTCCCCTTGCTCGTAAAATTACCGGGATTCCCACAGGAATTCGGCACCTCCAGAGGATTGGCATTGGACTGGTACTCACTGCTGCATCAATGGCCATTGCAGGGGTCGTGGAGACTCGCCGCAAAAATGTTGCAATCAAACACAATATGGTGGATAGTGTGGAGCCTTTGCCGATGAGCGTTTTCTGGTTGGGATTTCAGTTTTGCATATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAAAGCTCAGCAGGGATGAAGTCATTGAGCACAGCCATCGCTTGGTGTTCGATTGCGTTTGGGTACTTCACAAGTACGGTAGTGGTGGAGGTGGTGAACAAGGCGAGCGGAGGATGGCTGGCAAGCAACAATCTCAACAGAGATAACTTGAACTATTTCTACTGGTTACTGTCAGTGTTGAGCGTGCTGAATTTTGGGTTTTATTTGGTTTGTGCTTCTTGGTATAGGTATAAGAATGTTGAGGTCCTTTGA

mRNA sequence

ATGGCATTTGTGTCGATGGCGCTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCGGTGACCACTCTCACCAATTTCATGGGAACCACGTTTCTTCTCACACTCGTCGGCGGTTTCGTCTCCGAAACTTATCTCTCAAGATTCAAGACTTGCGTCCTCTTCGCTTCCTTTGAGTTCATGGCACATTTCCCGCACCTAAGGCCGGCACCGTGCAAAGGAGCGGCGGCGGGCAGCAAGTGCGAGGCGGCGGACAGGGGTCAGGAGGCGATTCTTTACGTGGGAATATACCTGATAGCGCTGGGAGCGAGTGGCGTGAAAGTGGCGCTGCCAGCATTGGGGGCGGATCAATTCGACGACAGAGATCCAGTGGAGGCGGCGAAGCTGTCGAGCTTTTTCAACTGGTTCCTGTTCAGTCTGACGATAGGATCGATGGTGGGAGTGACATTGATAGCGTGGATAAACACAGCGGTGGGTTGGGACTTGGCGTTTGTGGTTTGTAGCATTGCACTTCTTGCTGCCATTGTTGTTGTGCTTGCAGGCAAGTCGTTTTATCGCAATAATGTGCCTATTGGAAGCCCCGTTCTCAGACTTCTTCAGGTCTTTGTAGCTTCAATCCGAAACAGGAAACTTCCACTTCCAGAGAATGTTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAATCCTCGAGAAAACTGATCAATTCAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAAGTGCATCAATGTCAGCAAATCAGCTAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAAGAAACAAAGATTCTAATCAGAATGCTCCCAATTATACTAAGCACCATCTTCATGGCCACCTGCATGGCTCAACTCCAAACATTCTCCATTCAACAAAGCATAACCATGGATTGCCATTTCCTGGGATTCAAAATTCCAGGCCCCTCTGTCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTATTTTCGTTCCCCTTGCTCGTAAAATTACCGGGATTCCCACAGGAATTCGGCACCTCCAGAGGATTGGCATTGGACTGGTACTCACTGCTGCATCAATGGCCATTGCAGGGGTCGTGGAGACTCGCCGCAAAAATGTTGCAATCAAACACAATATGGTGGATAGTGTGGAGCCTTTGCCGATGAGCGTTTTCTGGTTGGGATTTCAGTTTTGCATATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAAAGCTCAGCAGGGATGAAGTCATTGAGCACAGCCATCGCTTGGTGTTCGATTGCGTTTGGGTACTTCACAAGTACGGTAGTGGTGGAGGTGGTGAACAAGGCGAGCGGAGGATGGCTGGCAAGCAACAATCTCAACAGAGATAACTTGAACTATTTCTACTGGTTACTGTCAGTGTTGAGCGTGCTGAATTTTGGGTTTTATTTGGTTTGTGCTTCTTGGTATAGGTATAAGAATGTTGAGGTCCTTTGA

Coding sequence (CDS)

ATGGCATTTGTGTCGATGGCGCTGAGCCTTGTGACGTATTTCAGTGGATATATGAACTTCAGCTTGACAAAATCGGTGACCACTCTCACCAATTTCATGGGAACCACGTTTCTTCTCACACTCGTCGGCGGTTTCGTCTCCGAAACTTATCTCTCAAGATTCAAGACTTGCGTCCTCTTCGCTTCCTTTGAGTTCATGGCACATTTCCCGCACCTAAGGCCGGCACCGTGCAAAGGAGCGGCGGCGGGCAGCAAGTGCGAGGCGGCGGACAGGGGTCAGGAGGCGATTCTTTACGTGGGAATATACCTGATAGCGCTGGGAGCGAGTGGCGTGAAAGTGGCGCTGCCAGCATTGGGGGCGGATCAATTCGACGACAGAGATCCAGTGGAGGCGGCGAAGCTGTCGAGCTTTTTCAACTGGTTCCTGTTCAGTCTGACGATAGGATCGATGGTGGGAGTGACATTGATAGCGTGGATAAACACAGCGGTGGGTTGGGACTTGGCGTTTGTGGTTTGTAGCATTGCACTTCTTGCTGCCATTGTTGTTGTGCTTGCAGGCAAGTCGTTTTATCGCAATAATGTGCCTATTGGAAGCCCCGTTCTCAGACTTCTTCAGGTCTTTGTAGCTTCAATCCGAAACAGGAAACTTCCACTTCCAGAGAATGTTAATGAACTACATGAGATTCGTGACAAAGAAGCTGCAATCCCATATGAAATCCTCGAGAAAACTGATCAATTCAGATTCTTAGACAGAGCAGCCATTATAAGGAATGATACAAGTGCATCAATGTCAGCAAATCAGCTAGGACCATGGAGACTCTGCACAGTGACACAGGTTGAAGAAACAAAGATTCTAATCAGAATGCTCCCAATTATACTAAGCACCATCTTCATGGCCACCTGCATGGCTCAACTCCAAACATTCTCCATTCAACAAAGCATAACCATGGATTGCCATTTCCTGGGATTCAAAATTCCAGGCCCCTCTGTCCCCGTCATTCCCTTGCTTTTCATGTTCTTCTTTATCCCCTTTTATGAGCGTATTTTCGTTCCCCTTGCTCGTAAAATTACCGGGATTCCCACAGGAATTCGGCACCTCCAGAGGATTGGCATTGGACTGGTACTCACTGCTGCATCAATGGCCATTGCAGGGGTCGTGGAGACTCGCCGCAAAAATGTTGCAATCAAACACAATATGGTGGATAGTGTGGAGCCTTTGCCGATGAGCGTTTTCTGGTTGGGATTTCAGTTTTGCATATTTGGAATGGGCGATATATTCACTCTTGTAGGACTGCTGGAATTCTTTTATGCAGAAAGCTCAGCAGGGATGAAGTCATTGAGCACAGCCATCGCTTGGTGTTCGATTGCGTTTGGGTACTTCACAAGTACGGTAGTGGTGGAGGTGGTGAACAAGGCGAGCGGAGGATGGCTGGCAAGCAACAATCTCAACAGAGATAACTTGAACTATTTCTACTGGTTACTGTCAGTGTTGAGCGTGCTGAATTTTGGGTTTTATTTGGTTTGTGCTTCTTGGTATAGGTATAAGAATGTTGAGGTCCTTTGA

Protein sequence

MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLFASFEFMAHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVKVALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVCSIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKEAAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL
Homology
BLAST of Lsi08G014340 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 4.7e-102
Identity = 212/541 (39.19%), Postives = 322/541 (59.52%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           +A+++ A +LV Y   YM+ S +KS   +TNFMGT FLL L+GGF+S+ + S F+  ++ 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EF+        A  P L P  C        CE     + A+L+VG+YL+ALG  G+K
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSCDSPT----CEEVSGSKAAMLFVGLYLVALGVGGIK 163

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            +L + GA+QFD+  P    + S+FFN+F+F L  G++V VT + W+    GW+  F V 
Sbjct: 164 GSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVS 223

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVA---------SIRNRKLPLPENVN 240
           +IA+  +I++ L+G  FYRN +P GSP+  +L+V +A         S  N    +  + +
Sbjct: 224 TIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS 283

Query: 241 ELHEIRDKEAAIPYEILEKTDQFRFLDRAAIIRNDTSA-SMSANQLGPWRL--CTVTQVE 300
                + K+       LEK  Q   L   A + N     + +A++    RL  CTV QVE
Sbjct: 284 NHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVE 343

Query: 301 ETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFF 360
           + KI+++MLPI   TI +  C+AQL TFS+QQ+ +M+      KIP  S+P+ P++F+  
Sbjct: 344 DVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMI 403

Query: 361 FIPFYERIFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMV 420
             P Y+ + +P ARK T   TG+ HLQRIG+GLVL+  +MA+A +VE +RK VA    ++
Sbjct: 404 LAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLL 463

Query: 421 DSVEPLPMSVFWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYF 480
           DS E LP++  W+  Q+   G  D+FTL GLLE+F+ E+ + M+SL+T+++W S+A GY+
Sbjct: 464 DSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYY 523

Query: 481 TSTVVVEVVNKASGG-----WLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYK 517
            S+V+V +VN  +G      WL   ++NR  L+YFYWL+ VLS  NF  YL  A  Y+Y+
Sbjct: 524 LSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 580

BLAST of Lsi08G014340 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 3.0e-101
Identity = 216/529 (40.83%), Postives = 317/529 (59.92%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MA  ++  +L+TY    M+F L+KS   +TNF+GT FLL+L+GGF+S++YL  F+T ++F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  ASFEF--------MAHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
              E          AH P LRP  C   +    C  A+  + A LY  + L+ALG+  +K
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
             + + GA+QF  +D     KLSSFFN   F+ ++G ++ +TL+ W+ T  G D+ F V 
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           +  + A ++ ++AG SFYRN  P GS    + QVFVA+I  RK   P N N +H+     
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQ--PST 296

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
             +  + L  +++FRFLD+A I       +       PWRLCT+ QV + KIL+ ++PI 
Sbjct: 297 DLVRVKPLLHSNKFRFLDKACI------KTQGKAMESPWRLCTIEQVHQVKILLSVIPIF 356

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFL-GFKIPGPSVPVIPLLFMFFFIPFYERIFVP 360
             TI   T +AQLQTFS+QQ  +M+ H    F+IP  S+  IP + + FF+P YE  FVP
Sbjct: 357 ACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVP 416

Query: 361 LARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVF 420
           LARK+TG  +GI  LQRIG GL L   SM  A +VE +R+   ++ N++       +S+F
Sbjct: 417 LARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM-------LSIF 476

Query: 421 WLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNK 480
           W+  QF IFG+ ++FT VGL+EFFY +SS  M+S  TA+ +CS +FG++ S+V+V  VN+
Sbjct: 477 WIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNR 536

Query: 481 ASG--------GWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWY 513
            +         GWL  N+LN+D L++FYWLL+ LS +NF  YL  + WY
Sbjct: 537 VTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567

BLAST of Lsi08G014340 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.1e-100
Identity = 210/529 (39.70%), Postives = 322/529 (60.87%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL L+GGF+++ + S F   ++ 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EF+        A  P L P PCK +AA  +CE     + A L+VG+YL++LG  G+K
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSSAA-LRCEVVGGSKAAFLFVGLYLVSLGIGGIK 165

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V VT + WI    GW+  F V 
Sbjct: 166 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 225

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFV-ASIRNRKLPLPENVNELHEIR-D 240
           +I++  +I+V L G  FY+N +P GSP+  + +V + ASI +       N     E++ +
Sbjct: 226 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 285

Query: 241 KEAAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          +       W  CTV QVE+ KI+++MLP
Sbjct: 286 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 345

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFV 360
           I   TI +  C+AQL T+S+ Q+ TM+   + F +P  S+PV P++FM    P Y+ + +
Sbjct: 346 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 405

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A +VE +RK VA +  ++DS E LP++ 
Sbjct: 406 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 465

Query: 421 FWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W S+A GY+ S+V+V +VN
Sbjct: 466 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 525

Query: 481 KAS-----GGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRY 515
           + +       WL    LNR+ L+ FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 526 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Lsi08G014340 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 2.2e-96
Identity = 209/527 (39.66%), Postives = 309/527 (58.63%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           M   ++  +L+TY    M+F L+K+   +TNF+GT F+  L+GG++S+ +L  F T ++F
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  ASFEF--------MAHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
              E          AH P L+P  C        CE A   +  I ++ +YL+ALG+  VK
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKC-NPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVK 179

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
             + A GADQF    P ++ +LSS+FN   F+ ++G ++ +TL+ W+ T  G D+ F V 
Sbjct: 180 PNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVS 239

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELH-EIRDK 240
           + A+   I+ +++G  ++RN  P  S    +  V VA+I  RKL  P +   LH +    
Sbjct: 240 AAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVA 299

Query: 241 EAAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPI 300
              +P   L  T +FRFLD+A I   DT+   S     PWRLCTVTQVE+ K LI ++PI
Sbjct: 300 NDVVPSSTLPHTPRFRFLDKACIKIQDTNTKES-----PWRLCTVTQVEQVKTLISLVPI 359

Query: 301 ILSTIFMATCMAQLQTFSIQQSITMDCHFL-GFKIPGPSVPVIPLLFMFFFIPFYERIFV 360
             STI   T +AQLQTFS+QQ  +M+      F IP  S+  IP + + F +P Y+   V
Sbjct: 360 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 419

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSV 420
           P ARK+TG  +GI  L RIGIGL L+  SM  A ++E +R++ ++    +       +S+
Sbjct: 420 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSI 479

Query: 421 FWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVN 480
           FW+  QF IFG+ ++FT VGL+EFFY +S+ GM+S   A+ +CS +FG++ S+V+V VVN
Sbjct: 480 FWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVN 539

Query: 481 KA------SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASW 512
           K       S GWL  N+LN+D L+ FYWLL+VLS+LNF  YL  + W
Sbjct: 540 KITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of Lsi08G014340 vs. ExPASy Swiss-Prot
Match: Q9SX20 (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 4.7e-94
Identity = 212/537 (39.48%), Postives = 316/537 (58.85%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           +A V    ++++Y +  ++  LTK+  TLTNF GT+ L  L+G F+++++  RF T + F
Sbjct: 42  LAVVGFHANMISYLTTQLHLPLTKAANTLTNFAGTSSLTPLLGAFIADSFAGRFWT-ITF 101

Query: 61  ASFEFM---------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGV 120
           AS  +          A  P LRP PCKG      C  AD  Q +ILYV + L ALG+ G+
Sbjct: 102 ASIIYQIGMTLLTISAIIPTLRPPPCKGEEV---CVVADTAQLSILYVALLLGALGSGGI 161

Query: 121 KVALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVV 180
           +  + A GADQFD+ DP +  K  ++FNW+ F +    ++ VT++ WI   VGW L   +
Sbjct: 162 RPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCMGAAVLLAVTVLVWIQDNVGWGLGLGI 221

Query: 181 CSIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDK 240
            ++A+  +++  + G   YR+ VP GSP  RL+QV VA+ R RKL +  + + L+   + 
Sbjct: 222 PTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQVGVAAFRKRKLRMVSDPSLLYFNDEI 281

Query: 241 EAAIPY-EILEKTDQFRFLDRAAIIRNDTSASMSANQL-GPWRLCTVTQVEETKILIRML 300
           +A I     L  T    FLD+AAI+  +   ++   Q+   WRL TV +VEE K +IRM 
Sbjct: 282 DAPISLGGKLTHTKHMSFLDKAAIVTEED--NLKPGQIPNHWRLSTVHRVEELKSVIRMG 341

Query: 301 PIILSTIFMATCMAQLQTFSIQQSITMDCHFL-GFKIPGPSVPVIPLLFMFFFIPFYERI 360
           PI  S I + T  AQ  TFS+QQ+ TM+ H    F+IP  S+ V   + M   I FY+R+
Sbjct: 342 PIGASGILLITAYAQQGTFSLQQAKTMNRHLTNSFQIPAGSMSVFTTVAMLTTIIFYDRV 401

Query: 361 FVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPL-P 420
           FV +ARK TG+  GI  L R+GIG V++  +  +AG VE +RK+VAI+H ++D    + P
Sbjct: 402 FVKVARKFTGLERGITFLHRMGIGFVISIIATLVAGFVEVKRKSVAIEHGLLDKPHTIVP 461

Query: 421 MSVFWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVE 480
           +S  WL  Q+ + G+ + F  +G LEFFY ++   M+S +TA+ W +I+ G + ST++V 
Sbjct: 462 ISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPESMRSTATALFWMAISIGNYVSTLLVT 521

Query: 481 VVNKAS-----GGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEV 520
           +V+K S       WL  NNLNR  L YFYWL++VL  +N  +YL CA  Y YK V+V
Sbjct: 522 LVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITVLQAVNLVYYLWCAKIYTYKPVQV 572

BLAST of Lsi08G014340 vs. ExPASy TrEMBL
Match: A0A6J1CR11 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 2.1e-251
Identity = 454/528 (85.98%), Postives = 483/528 (91.48%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTL+GGF+S+TYLSRFKTC++F
Sbjct: 37  MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 96

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EFM        AHF  LRPAPCKGA A  +CEAAD GQEAILY+GIYLIALG SGVK
Sbjct: 97  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 156

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            ALPALGADQFD  DP EA KLSSFFNWFLFSLT GS+VG+TLI WINT VGWD AFVVC
Sbjct: 157 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 216

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           S+A++AAIV V +GK FYRNN P GSP+ R+ QVFVASIRNRKLPLP+N +ELHEIRDKE
Sbjct: 217 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 276

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN TSASM+A Q GPWRLCTVTQVEETKILIRMLPII
Sbjct: 277 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 336

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           +STIFMATCMAQLQTFSIQQSITMD +FLGFKIPGPSVPVIPLLFM F IPFYER+FVPL
Sbjct: 337 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 396

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAG VETRRKN+AI+H+MVDS EPLPMSVFW
Sbjct: 397 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 456

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           L FQFCIFGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 457 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 516

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           SGGWL SNNLNRD LNYFYWLLSVLSVLNFGFYL+CASWYRYKNVE+L
Sbjct: 517 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEIL 563

BLAST of Lsi08G014340 vs. ExPASy TrEMBL
Match: A0A6J1CSN2 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 877.9 bits (2267), Expect = 2.1e-251
Identity = 454/528 (85.98%), Postives = 483/528 (91.48%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTL+GGF+S+TYLSRFKTC++F
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EFM        AHF  LRPAPCKGA A  +CEAAD GQEAILY+GIYLIALG SGVK
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 120

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            ALPALGADQFD  DP EA KLSSFFNWFLFSLT GS+VG+TLI WINT VGWD AFVVC
Sbjct: 121 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 180

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           S+A++AAIV V +GK FYRNN P GSP+ R+ QVFVASIRNRKLPLP+N +ELHEIRDKE
Sbjct: 181 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 240

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN TSASM+A Q GPWRLCTVTQVEETKILIRMLPII
Sbjct: 241 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 300

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           +STIFMATCMAQLQTFSIQQSITMD +FLGFKIPGPSVPVIPLLFM F IPFYER+FVPL
Sbjct: 301 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 360

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAG VETRRKN+AI+H+MVDS EPLPMSVFW
Sbjct: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 420

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           L FQFCIFGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 421 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           SGGWL SNNLNRD LNYFYWLLSVLSVLNFGFYL+CASWYRYKNVE+L
Sbjct: 481 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEIL 527

BLAST of Lsi08G014340 vs. ExPASy TrEMBL
Match: A0A6J1KKU7 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 1.0e-248
Identity = 451/528 (85.42%), Postives = 480/528 (90.91%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFV++ALSL+TYFSGYMNFSLTKS TT+TNFMGTTFLLTLVGGFVS+TYLSRFKTCVLF
Sbjct: 49  MAFVALALSLMTYFSGYMNFSLTKSATTMTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLF 108

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           A FE +        AHF  LRP PCKGA AGS+C+AA+ GQEAILYVGIYLIALGASGVK
Sbjct: 109 ACFELLGYFLLSAQAHFRELRPEPCKGALAGSECKAANGGQEAILYVGIYLIALGASGVK 168

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
           VALPALGADQF++ DP EA KLSSFFNWFLFSLT GS VG+TLI WINT VGWD AFVVC
Sbjct: 169 VALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVC 228

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           +IA+LAAI+VV AGKSF+RNNVP GSP+LR+LQV VASI NRKLPLPEN +ELHEIRDKE
Sbjct: 229 TIAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIHNRKLPLPENADELHEIRDKE 288

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           A IP EILEKTDQFRFL+RAAIIRNDT           WRLCTVTQVEETKILIRMLPII
Sbjct: 289 ADIPIEILEKTDQFRFLERAAIIRNDTRTL--------WRLCTVTQVEETKILIRMLPII 348

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           +STIFMATCMAQLQTFSIQQS+TMD +FLGFKIPGPSVPVIPLLFMFFFIPFYER+FVPL
Sbjct: 349 VSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPGPSVPVIPLLFMFFFIPFYERVFVPL 408

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIG GL+LTAASMAIAG VETRRKNVAI+HNMVDS EPLPMSVFW
Sbjct: 409 ARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSEEPLPMSVFW 468

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           L FQFC+FGM DIFT+VGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 469 LSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 528

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           SGGWLASNNLNRDNLNYFYWLLSVLSV+NFGFYLVCASWYRYKNVEVL
Sbjct: 529 SGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVL 568

BLAST of Lsi08G014340 vs. ExPASy TrEMBL
Match: A0A6J1GIY5 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.9e-248
Identity = 449/528 (85.04%), Postives = 480/528 (90.91%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFV++ALSL+TYF+GYMNFSLTKS TTLTNFMGTTFLLTLVGGFVS+TYLSRFKTCVLF
Sbjct: 34  MAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLF 93

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           A FE +        AHF  LRP PCKGAAAGS+C+AA+ GQEAILYVGIYLIALGASGVK
Sbjct: 94  ACFELLGYCLLTAQAHFRELRPEPCKGAAAGSECKAANGGQEAILYVGIYLIALGASGVK 153

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
           VALPALGADQF++ DP EA KLSSFFNWFLFSLT GS VG+TLI WINT VGWD AFVVC
Sbjct: 154 VALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVC 213

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           + A+LAAI+VV AGKSF+RNNVP GSP+LR+LQV VASIRNRKLPLP N +ELHEIRDKE
Sbjct: 214 TAAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRDKE 273

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           A IP EILEKTDQFRFL+RAAIIRNDT          PWRLCTVTQVEETKILIRMLPII
Sbjct: 274 ADIPIEILEKTDQFRFLERAAIIRNDTRT--------PWRLCTVTQVEETKILIRMLPII 333

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           +STIFMA+CMAQLQTFSIQQS+TMD +F+GFKIPGPSVPVIPLLFMFFFIPFYER+FVPL
Sbjct: 334 VSTIFMASCMAQLQTFSIQQSLTMDSNFIGFKIPGPSVPVIPLLFMFFFIPFYERVFVPL 393

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIG GL+LTAASMAIAG VETRRKNVAI+HNMVDS EPLPMSVFW
Sbjct: 394 ARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSEEPLPMSVFW 453

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           L FQFC+FGM DIFT+VGLLEFFYAESSA MKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 454 LSFQFCVFGMADIFTMVGLLEFFYAESSAEMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 513

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           SGGWLASNNLNRDNLNYFYWLLSVLSV+NFGFYLVCASWYRYKNVEVL
Sbjct: 514 SGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVL 553

BLAST of Lsi08G014340 vs. ExPASy TrEMBL
Match: A0A0A0LLC0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360700 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 3.3e-228
Identity = 404/453 (89.18%), Postives = 423/453 (93.38%), Query Frame = 0

Query: 67  AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVKVALPALGADQFDDR 126
           AHFPHLRPAPCK A AGS+CEAA   QEA LY G+YLIALG SGVK ALPALGADQFDD+
Sbjct: 21  AHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVKAALPALGADQFDDK 80

Query: 127 DPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVCSIALLAAIVVVLAG 186
           DP EA K+SSFFNWFLFSLTIGS+VG+TLI WINT VGWD AFVVCSI++LAAI VV  G
Sbjct: 81  DPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVCSISVLAAIFVVFLG 140

Query: 187 KSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKEAAIPYEILEKTDQF 246
           KSFYR+NVP GSP+LR LQVFVASIRNRKLPLP N NELHEIRDKEAAIPYEILEKTDQF
Sbjct: 141 KSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKEAAIPYEILEKTDQF 200

Query: 247 RFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPIILSTIFMATCMAQLQ 306
           RFLDRAAIIRNDT AS+S NQ GPWRLCTVTQVEETKILIRMLPIILSTIFMATCMAQLQ
Sbjct: 201 RFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPIILSTIFMATCMAQLQ 260

Query: 307 TFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPLARKITGIPTGIRHL 366
           TFSIQQSITMD  FLGFKIPGPS+PVIPLLFMFFFIPFYER+FVPLARKITGIPTGIRHL
Sbjct: 261 TFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHL 320

Query: 367 QRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFWLGFQFCIFGMGDIF 426
           QRIGIGLVLTAASMAIAG VETRRKNVAIKHNMVDS EPLP+SVFWLGFQFC+FGMGDIF
Sbjct: 321 QRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFWLGFQFCVFGMGDIF 380

Query: 427 TLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDN 486
           TLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKASGGWLASNNLN+D 
Sbjct: 381 TLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKASGGWLASNNLNKDK 440

Query: 487 LNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEV 520
           L+YFYWLLSVLSVLNFGFYLVCASWYRYKNVE+
Sbjct: 441 LDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEI 473

BLAST of Lsi08G014340 vs. NCBI nr
Match: XP_038885750.1 (protein NRT1/ PTR FAMILY 4.5-like [Benincasa hispida])

HSP 1 Score: 927.2 bits (2395), Expect = 6.4e-266
Identity = 476/528 (90.15%), Postives = 497/528 (94.13%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTL+GGF+SETYL RFKTCVLF
Sbjct: 61  MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLPRFKTCVLF 120

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           ASFE M        AHFPHLRPAPCKGAAA S+CEAA  GQEAILYVG+YLIALG SGVK
Sbjct: 121 ASFELMGYCLLTAQAHFPHLRPAPCKGAAARSECEAAGSGQEAILYVGLYLIALGTSGVK 180

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            ALPALGADQFDDRDP EAAK+SSFFNWFLFSLTIGS+VGVTLI WINT VGWD AFVVC
Sbjct: 181 AALPALGADQFDDRDPAEAAKVSSFFNWFLFSLTIGSIVGVTLIVWINTEVGWDWAFVVC 240

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           SIA+L A+VVV AGKSFYRNNVP GSP+LRLLQVFVASIRNRKLPLPENVNELHEIRDKE
Sbjct: 241 SIAILVAVVVVFAGKSFYRNNVPKGSPILRLLQVFVASIRNRKLPLPENVNELHEIRDKE 300

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           AAIP+EILEKTDQFRFLDRAAI+RNDT+ASMSANQ GPWRLCTVTQVEETKILIRMLPII
Sbjct: 301 AAIPHEILEKTDQFRFLDRAAIVRNDTNASMSANQPGPWRLCTVTQVEETKILIRMLPII 360

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           +STIFMATCMAQLQTFSIQQSITMDC FLGFKIPGPSVPVIPLLFM FFIPFYE +FVPL
Sbjct: 361 VSTIFMATCMAQLQTFSIQQSITMDCQFLGFKIPGPSVPVIPLLFMIFFIPFYEHVFVPL 420

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIGIGLV+T AS+AIA VVETRRKN+AIKHNMVDS EPLPM+VFW
Sbjct: 421 ARKITGIPTGIRHLQRIGIGLVITTASIAIAAVVETRRKNIAIKHNMVDSEEPLPMTVFW 480

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           LGFQFC+FGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 481 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 540

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           SGGWLASNNLNRDNL+YFYWLLSVLSVLNFGFYLVCASWY YKNV VL
Sbjct: 541 SGGWLASNNLNRDNLDYFYWLLSVLSVLNFGFYLVCASWYTYKNVGVL 588

BLAST of Lsi08G014340 vs. NCBI nr
Match: XP_011649593.2 (protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus])

HSP 1 Score: 903.3 bits (2333), Expect = 9.9e-259
Identity = 464/527 (88.05%), Postives = 487/527 (92.41%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTL+GGF+SETYLSRFKTC+LF
Sbjct: 25  MAFVSVALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLLGGFISETYLSRFKTCLLF 84

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           A FE M        AHFPHLRPAPCK A AGS+CEAA   QEA LY G+YLIALG SGVK
Sbjct: 85  AFFELMGYCLLTVQAHFPHLRPAPCKEAPAGSECEAAGSSQEAFLYTGLYLIALGTSGVK 144

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            ALPALGADQFDD+DP EA K+SSFFNWFLFSLTIGS+VG+TLI WINT VGWD AFVVC
Sbjct: 145 AALPALGADQFDDKDPSEAGKVSSFFNWFLFSLTIGSIVGLTLIVWINTEVGWDWAFVVC 204

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           SI++LAAI VV  GKSFYR+NVP GSP+LR LQVFVASIRNRKLPLP N NELHEIRDKE
Sbjct: 205 SISVLAAIFVVFLGKSFYRHNVPQGSPILRFLQVFVASIRNRKLPLPVNANELHEIRDKE 264

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           AAIPYEILEKTDQFRFLDRAAIIRNDT AS+S NQ GPWRLCTVTQVEETKILIRMLPII
Sbjct: 265 AAIPYEILEKTDQFRFLDRAAIIRNDTIASISTNQQGPWRLCTVTQVEETKILIRMLPII 324

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           LSTIFMATCMAQLQTFSIQQSITMD  FLGFKIPGPS+PVIPLLFMFFFIPFYER+FVPL
Sbjct: 325 LSTIFMATCMAQLQTFSIQQSITMDSQFLGFKIPGPSIPVIPLLFMFFFIPFYERVFVPL 384

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAG VETRRKNVAIKHNMVDS EPLP+SVFW
Sbjct: 385 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNVAIKHNMVDSTEPLPISVFW 444

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           LGFQFC+FGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWC+IAFGYFTSTVVV VVNKA
Sbjct: 445 LGFQFCVFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCAIAFGYFTSTVVVTVVNKA 504

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEV 520
           SGGWLASNNLN+D L+YFYWLLSVLSVLNFGFYLVCASWYRYKNVE+
Sbjct: 505 SGGWLASNNLNKDKLDYFYWLLSVLSVLNFGFYLVCASWYRYKNVEI 551

BLAST of Lsi08G014340 vs. NCBI nr
Match: KAG6585639.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 878.6 bits (2269), Expect = 2.6e-251
Identity = 450/520 (86.54%), Postives = 481/520 (92.50%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFV++ALSL+TYF+GYMNFSLTKS TTLTNFMGTTFLLTLVGGFVS+TYLSRFKTCVLF
Sbjct: 283 MAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLF 342

Query: 61  ASFEFMAHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVKVALPALGA 120
           A FE +AHF  LRP PCKG AAGS+C+AA+ GQEAILYVGIYLIALGASGVKVALPALGA
Sbjct: 343 ACFELLAHFRELRPEPCKGTAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGA 402

Query: 121 DQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVCSIALLAAI 180
           DQF++ DP EA KLSSFFNWFLFSLT GS VG+TLI WINT VGWD AFVVC+ A+LAAI
Sbjct: 403 DQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTAAVLAAI 462

Query: 181 VVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKEAAIPYEIL 240
           +VV +GKSF+RNNVP GSP+LR+LQV VASIRNRKLPLP NV+ELHEIR KEA IP EIL
Sbjct: 463 LVVCSGKSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNVDELHEIRGKEADIPIEIL 522

Query: 241 EKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPIILSTIFMAT 300
           EKTDQFRFL+RAAIIRNDT          PWRLCTVTQVEETKILIRMLPII+STIFMAT
Sbjct: 523 EKTDQFRFLERAAIIRNDTRT--------PWRLCTVTQVEETKILIRMLPIIVSTIFMAT 582

Query: 301 CMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPLARKITGIP 360
           CMAQLQTFSIQQS+TMDC+F+GFKIPGPSVPVIPLLFMFFFIPFYER+FVPLARKITGIP
Sbjct: 583 CMAQLQTFSIQQSLTMDCNFIGFKIPGPSVPVIPLLFMFFFIPFYERVFVPLARKITGIP 642

Query: 361 TGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFWLGFQFCIF 420
           TGIRHLQRIG GL+LTAASMAIAG VETRRKNVAI+HNMVDS EPLPMSVFWL FQFC+F
Sbjct: 643 TGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVF 702

Query: 421 GMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLASN 480
           GM DIFT+VGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLASN
Sbjct: 703 GMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLASN 762

Query: 481 NLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           NLNRDNLNYFYWLLSVLSV+NFGFYLVCASWYRYKNVEVL
Sbjct: 763 NLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVL 794

BLAST of Lsi08G014340 vs. NCBI nr
Match: XP_022144216.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Momordica charantia])

HSP 1 Score: 877.9 bits (2267), Expect = 4.4e-251
Identity = 454/528 (85.98%), Postives = 483/528 (91.48%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTL+GGF+S+TYLSRFKTC++F
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EFM        AHF  LRPAPCKGA A  +CEAAD GQEAILY+GIYLIALG SGVK
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 120

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            ALPALGADQFD  DP EA KLSSFFNWFLFSLT GS+VG+TLI WINT VGWD AFVVC
Sbjct: 121 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 180

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           S+A++AAIV V +GK FYRNN P GSP+ R+ QVFVASIRNRKLPLP+N +ELHEIRDKE
Sbjct: 181 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 240

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN TSASM+A Q GPWRLCTVTQVEETKILIRMLPII
Sbjct: 241 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 300

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           +STIFMATCMAQLQTFSIQQSITMD +FLGFKIPGPSVPVIPLLFM F IPFYER+FVPL
Sbjct: 301 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 360

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAG VETRRKN+AI+H+MVDS EPLPMSVFW
Sbjct: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 420

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           L FQFCIFGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 421 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           SGGWL SNNLNRD LNYFYWLLSVLSVLNFGFYL+CASWYRYKNVE+L
Sbjct: 481 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEIL 527

BLAST of Lsi08G014340 vs. NCBI nr
Match: XP_022144215.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 [Momordica charantia])

HSP 1 Score: 877.9 bits (2267), Expect = 4.4e-251
Identity = 454/528 (85.98%), Postives = 483/528 (91.48%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MAFVS+ALSLVTYFSGYMNFSLTKS TTLTNFMGTTFLLTL+GGF+S+TYLSRFKTC++F
Sbjct: 37  MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 96

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EFM        AHF  LRPAPCKGA A  +CEAAD GQEAILY+GIYLIALG SGVK
Sbjct: 97  ASIEFMGYGLLTAQAHFRELRPAPCKGAVA-RECEAADGGQEAILYLGIYLIALGTSGVK 156

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            ALPALGADQFD  DP EA KLSSFFNWFLFSLT GS+VG+TLI WINT VGWD AFVVC
Sbjct: 157 SALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVC 216

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           S+A++AAIV V +GK FYRNN P GSP+ R+ QVFVASIRNRKLPLP+N +ELHEIRDKE
Sbjct: 217 SLAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKE 276

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
           AA+PYEILEKTDQFRFLDRAAIIRN TSASM+A Q GPWRLCTVTQVEETKILIRMLPII
Sbjct: 277 AAMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPII 336

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFVPL 360
           +STIFMATCMAQLQTFSIQQSITMD +FLGFKIPGPSVPVIPLLFM F IPFYER+FVPL
Sbjct: 337 VSTIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPL 396

Query: 361 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVFW 420
           ARKITGIPTGIRHLQRIGIGLVLTAASMAIAG VETRRKN+AI+H+MVDS EPLPMSVFW
Sbjct: 397 ARKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFW 456

Query: 421 LGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 480
           L FQFCIFGMGD+FTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA
Sbjct: 457 LSFQFCIFGMGDMFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNKA 516

Query: 481 SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYKNVEVL 521
           SGGWL SNNLNRD LNYFYWLLSVLSVLNFGFYL+CASWYRYKNVE+L
Sbjct: 517 SGGWLESNNLNRDKLNYFYWLLSVLSVLNFGFYLMCASWYRYKNVEIL 563

BLAST of Lsi08G014340 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 373.2 bits (957), Expect = 3.3e-103
Identity = 212/541 (39.19%), Postives = 322/541 (59.52%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           +A+++ A +LV Y   YM+ S +KS   +TNFMGT FLL L+GGF+S+ + S F+  ++ 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EF+        A  P L P  C        CE     + A+L+VG+YL+ALG  G+K
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSCDSPT----CEEVSGSKAAMLFVGLYLVALGVGGIK 163

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            +L + GA+QFD+  P    + S+FFN+F+F L  G++V VT + W+    GW+  F V 
Sbjct: 164 GSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVS 223

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVA---------SIRNRKLPLPENVN 240
           +IA+  +I++ L+G  FYRN +P GSP+  +L+V +A         S  N    +  + +
Sbjct: 224 TIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPS 283

Query: 241 ELHEIRDKEAAIPYEILEKTDQFRFLDRAAIIRNDTSA-SMSANQLGPWRL--CTVTQVE 300
                + K+       LEK  Q   L   A + N     + +A++    RL  CTV QVE
Sbjct: 284 NHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQVE 343

Query: 301 ETKILIRMLPIILSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFF 360
           + KI+++MLPI   TI +  C+AQL TFS+QQ+ +M+      KIP  S+P+ P++F+  
Sbjct: 344 DVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFIMI 403

Query: 361 FIPFYERIFVPLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMV 420
             P Y+ + +P ARK T   TG+ HLQRIG+GLVL+  +MA+A +VE +RK VA    ++
Sbjct: 404 LAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSGLL 463

Query: 421 DSVEPLPMSVFWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYF 480
           DS E LP++  W+  Q+   G  D+FTL GLLE+F+ E+ + M+SL+T+++W S+A GY+
Sbjct: 464 DSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMGYY 523

Query: 481 TSTVVVEVVNKASGG-----WLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRYK 517
            S+V+V +VN  +G      WL   ++NR  L+YFYWL+ VLS  NF  YL  A  Y+Y+
Sbjct: 524 LSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYKYR 580

BLAST of Lsi08G014340 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.1e-102
Identity = 216/529 (40.83%), Postives = 317/529 (59.92%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           MA  ++  +L+TY    M+F L+KS   +TNF+GT FLL+L+GGF+S++YL  F+T ++F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  ASFEF--------MAHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
              E          AH P LRP  C   +    C  A+  + A LY  + L+ALG+  +K
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
             + + GA+QF  +D     KLSSFFN   F+ ++G ++ +TL+ W+ T  G D+ F V 
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELHEIRDKE 240
           +  + A ++ ++AG SFYRN  P GS    + QVFVA+I  RK   P N N +H+     
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQ--PST 296

Query: 241 AAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPII 300
             +  + L  +++FRFLD+A I       +       PWRLCT+ QV + KIL+ ++PI 
Sbjct: 297 DLVRVKPLLHSNKFRFLDKACI------KTQGKAMESPWRLCTIEQVHQVKILLSVIPIF 356

Query: 301 LSTIFMATCMAQLQTFSIQQSITMDCHFL-GFKIPGPSVPVIPLLFMFFFIPFYERIFVP 360
             TI   T +AQLQTFS+QQ  +M+ H    F+IP  S+  IP + + FF+P YE  FVP
Sbjct: 357 ACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVP 416

Query: 361 LARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSVF 420
           LARK+TG  +GI  LQRIG GL L   SM  A +VE +R+   ++ N++       +S+F
Sbjct: 417 LARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM-------LSIF 476

Query: 421 WLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVNK 480
           W+  QF IFG+ ++FT VGL+EFFY +SS  M+S  TA+ +CS +FG++ S+V+V  VN+
Sbjct: 477 WIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNR 536

Query: 481 ASG--------GWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWY 513
            +         GWL  N+LN+D L++FYWLL+ LS +NF  YL  + WY
Sbjct: 537 VTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567

BLAST of Lsi08G014340 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 8.1e-102
Identity = 210/529 (39.70%), Postives = 322/529 (60.87%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL L+GGF+++ + S F   ++ 
Sbjct: 42  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 101

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EF+        A  P L P PCK +AA  +CE     + A L+VG+YL++LG  G+K
Sbjct: 102 ASIEFLGLILLTIQARRPSLMPPPCKSSAA-LRCEVVGGSKAAFLFVGLYLVSLGIGGIK 161

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V VT + WI    GW+  F V 
Sbjct: 162 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 221

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFV-ASIRNRKLPLPENVNELHEIR-D 240
           +I++  +I+V L G  FY+N +P GSP+  + +V + ASI +       N     E++ +
Sbjct: 222 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 281

Query: 241 KEAAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          +       W  CTV QVE+ KI+++MLP
Sbjct: 282 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 341

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFV 360
           I   TI +  C+AQL T+S+ Q+ TM+   + F +P  S+PV P++FM    P Y+ + +
Sbjct: 342 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 401

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A +VE +RK VA +  ++DS E LP++ 
Sbjct: 402 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 461

Query: 421 FWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W S+A GY+ S+V+V +VN
Sbjct: 462 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 521

Query: 481 KAS-----GGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRY 515
           + +       WL    LNR+ L+ FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 522 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of Lsi08G014340 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 368.6 bits (945), Expect = 8.1e-102
Identity = 210/529 (39.70%), Postives = 322/529 (60.87%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           +AF++ A +LV Y   +M+ SL +S + +T FM T FLL L+GGF+++ + S F   ++ 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  ASFEFM--------AHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
           AS EF+        A  P L P PCK +AA  +CE     + A L+VG+YL++LG  G+K
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSSAA-LRCEVVGGSKAAFLFVGLYLVSLGIGGIK 165

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
            +LP+ GA+QFD+  P    + S+FFN+++F L+ G++V VT + WI    GW+  F V 
Sbjct: 166 GSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVS 225

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFV-ASIRNRKLPLPENVNELHEIR-D 240
           +I++  +I+V L G  FY+N +P GSP+  + +V + ASI +       N     E++ +
Sbjct: 226 TISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSCSSKTSSNHFTSREVQSE 285

Query: 241 KEAAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLP 300
            E   P + L  T+    L++A          +       W  CTV QVE+ KI+++MLP
Sbjct: 286 HEEKTPSQSL--TNSLTCLNKA----------IEGKTHHIWLECTVQQVEDVKIVLKMLP 345

Query: 301 IILSTIFMATCMAQLQTFSIQQSITMDCHFLGFKIPGPSVPVIPLLFMFFFIPFYERIFV 360
           I   TI +  C+AQL T+S+ Q+ TM+   + F +P  S+PV P++FM    P Y+ + +
Sbjct: 346 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 405

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSV 420
           P ARK+T    GI HLQRIG+GLVL+  +MA+A +VE +RK VA +  ++DS E LP++ 
Sbjct: 406 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 465

Query: 421 FWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVN 480
            W+  Q+   G  D+FTL GLLEFF+ E+ + M+SL+T+++W S+A GY+ S+V+V +VN
Sbjct: 466 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 525

Query: 481 KAS-----GGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASWYRY 515
           + +       WL    LNR+ L+ FYWL+ VLSV+NF  YL  A  Y+Y
Sbjct: 526 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Lsi08G014340 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 354.4 bits (908), Expect = 1.6e-97
Identity = 209/527 (39.66%), Postives = 309/527 (58.63%), Query Frame = 0

Query: 1   MAFVSMALSLVTYFSGYMNFSLTKSVTTLTNFMGTTFLLTLVGGFVSETYLSRFKTCVLF 60
           M   ++  +L+TY    M+F L+K+   +TNF+GT F+  L+GG++S+ +L  F T ++F
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  ASFEF--------MAHFPHLRPAPCKGAAAGSKCEAADRGQEAILYVGIYLIALGASGVK 120
              E          AH P L+P  C        CE A   +  I ++ +YL+ALG+  VK
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKC-NPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVK 179

Query: 121 VALPALGADQFDDRDPVEAAKLSSFFNWFLFSLTIGSMVGVTLIAWINTAVGWDLAFVVC 180
             + A GADQF    P ++ +LSS+FN   F+ ++G ++ +TL+ W+ T  G D+ F V 
Sbjct: 180 PNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVS 239

Query: 181 SIALLAAIVVVLAGKSFYRNNVPIGSPVLRLLQVFVASIRNRKLPLPENVNELH-EIRDK 240
           + A+   I+ +++G  ++RN  P  S    +  V VA+I  RKL  P +   LH +    
Sbjct: 240 AAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVA 299

Query: 241 EAAIPYEILEKTDQFRFLDRAAIIRNDTSASMSANQLGPWRLCTVTQVEETKILIRMLPI 300
              +P   L  T +FRFLD+A I   DT+   S     PWRLCTVTQVE+ K LI ++PI
Sbjct: 300 NDVVPSSTLPHTPRFRFLDKACIKIQDTNTKES-----PWRLCTVTQVEQVKTLISLVPI 359

Query: 301 ILSTIFMATCMAQLQTFSIQQSITMDCHFL-GFKIPGPSVPVIPLLFMFFFIPFYERIFV 360
             STI   T +AQLQTFS+QQ  +M+      F IP  S+  IP + + F +P Y+   V
Sbjct: 360 FASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDSFLV 419

Query: 361 PLARKITGIPTGIRHLQRIGIGLVLTAASMAIAGVVETRRKNVAIKHNMVDSVEPLPMSV 420
           P ARK+TG  +GI  L RIGIGL L+  SM  A ++E +R++ ++    +       +S+
Sbjct: 420 PFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLDGRI-------LSI 479

Query: 421 FWLGFQFCIFGMGDIFTLVGLLEFFYAESSAGMKSLSTAIAWCSIAFGYFTSTVVVEVVN 480
           FW+  QF IFG+ ++FT VGL+EFFY +S+ GM+S   A+ +CS +FG++ S+V+V VVN
Sbjct: 480 FWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVN 539

Query: 481 KA------SGGWLASNNLNRDNLNYFYWLLSVLSVLNFGFYLVCASW 512
           K       S GWL  N+LN+D L+ FYWLL+VLS+LNF  YL  + W
Sbjct: 540 KITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1574.7e-10239.19Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q56XQ63.0e-10140.83Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q8VYE41.1e-10039.70Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q93VV52.2e-9639.66Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q9SX204.7e-9439.48Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana OX=3702 GN=NPF3.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CR112.1e-25185.98protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1CSN22.1e-25185.98protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1KKU71.0e-24885.42protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE... [more]
A0A6J1GIY54.9e-24885.04protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 ... [more]
A0A0A0LLC03.3e-22889.18Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G360700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038885750.16.4e-26690.15protein NRT1/ PTR FAMILY 4.5-like [Benincasa hispida][more]
XP_011649593.29.9e-25988.05protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus][more]
KAG6585639.12.6e-25186.54Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022144216.14.4e-25185.98protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Momordica charantia][more]
XP_022144215.14.4e-25185.98protein NRT1/ PTR FAMILY 4.5-like isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G69850.13.3e-10339.19nitrate transporter 1:2 [more]
AT1G33440.12.1e-10240.83Major facilitator superfamily protein [more]
AT1G27040.28.1e-10239.70Major facilitator superfamily protein [more]
AT1G27040.18.1e-10239.70Major facilitator superfamily protein [more]
AT1G59740.11.6e-9739.66Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 68..473
e-value: 1.7E-84
score: 283.9
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 1..517
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..519
e-value: 1.2E-152
score: 510.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 4..515
NoneNo IPR availablePANTHERPTHR11654:SF409PROTEIN NRT1/ PTR FAMILY 4.5-LIKEcoord: 1..517

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G014340.1Lsi08G014340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042981 regulation of apoptotic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity