Lsi08G014260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G014260
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Allene oxide synthase
Location	chr08: 22492550 .. 22494148 (+)
RNA-Seq Expression	Lsi08G014260
Synteny	Lsi08G014260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGACTACCTCCATTATTATCCCTTCTCTTCAAACCCAGTTGCGATTCCCGTCGCAAGAAACGCCTCTGAGATCTCGTTCTAGAGTTGGGTTCGTTTCCCTACGCCCAATCTGCGCCGCCGACAGGGTTTCCTCCACGTCTTCCTCCTCTGTTCAAGTGCCGCAGCGGACTGTTTCGCCGCCGGAACCTACCAAGCTTCCTTTGAGGAAGATTCCAGGTGACTATGGGCCGCCGATGTTTGGGGCGTTGAAGGACCGACAAGACTATTTCTATAATCAGGGGAGGGAAGAGTATCTGAAATCTCGAATGCTTCGGTATGAATCCACTGTGTATAGAACCAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACAATTCTAAAGTTGAGAAGAAGGATCTCTTTACCGGGACTTACATGCCTTTAACAGAGCTCACCGGCGGTTACAGGGTTCTCTCTTATATTGACCCATCTGAGCCCGATCACGAGAAGCTTAAACAGCTCATCTTCTTCCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTCTCCGAGCTATTTGAGACTCTGGAGAAGGATTTGGCTGCTGCTGGTAAAGCCGAGTACAATGCTTCCGGTGAACAGGCGGCGTTTAATTTCTTGGCTCGGTCTCTCTTCGGAGCCAATCCGGTCGATACCAAATTGGGTCGTGACGCTCCGAAATTGATTTCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTCAGGCTCGGTCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGCTGATCAAAGCCGATTACCGGCGTCTGTATGACTTCTTTTACCAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGCCACAACTTGCTATTCACAACTTGTTTTAATTCATTCGGTGGGATGAAGATCTTATTCCCCAATATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTATATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAGGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAATGTTGTGTGGGTATCAGCCATTCGCGACCAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGATTCACCGGCGACGGTGAAGAGTTGCTGAAACACGTGCTATGGTCAAACGGACCAGAGACCCAGTCGCCGACGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTCTTCATCTCTCGCCTTCTCGTCGTCGAACTCTTCCTCCGGTACGACTCCTTCGACATCGAAGCCACAAACACTCCGTTAGGCGCCGCCGTCACTGTAACTTCCCTGAAGAAAGCAAGTTTCTGA

mRNA sequence

ATGTCGACTACCTCCATTATTATCCCTTCTCTTCAAACCCAGTTGCGATTCCCGTCGCAAGAAACGCCTCTGAGATCTCGTTCTAGAGTTGGGTTCGTTTCCCTACGCCCAATCTGCGCCGCCGACAGGGTTTCCTCCACGTCTTCCTCCTCTGTTCAAGTGCCGCAGCGGACTGTTTCGCCGCCGGAACCTACCAAGCTTCCTTTGAGGAAGATTCCAGGTGACTATGGGCCGCCGATGTTTGGGGCGTTGAAGGACCGACAAGACTATTTCTATAATCAGGGGAGGGAAGAGTATCTGAAATCTCGAATGCTTCGGTATGAATCCACTGTGTATAGAACCAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACAATTCTAAAGTTGAGAAGAAGGATCTCTTTACCGGGACTTACATGCCTTTAACAGAGCTCACCGGCGGTTACAGGGTTCTCTCTTATATTGACCCATCTGAGCCCGATCACGAGAAGCTTAAACAGCTCATCTTCTTCCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTCTCCGAGCTATTTGAGACTCTGGAGAAGGATTTGGCTGCTGCTGGTAAAGCCGAGTACAATGCTTCCGGTGAACAGGCGGCGTTTAATTTCTTGGCTCGGTCTCTCTTCGGAGCCAATCCGGTCGATACCAAATTGGGTCGTGACGCTCCGAAATTGATTTCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTCAGGCTCGGTCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGCTGATCAAAGCCGATTACCGGCGTCTGTATGACTTCTTTTACCAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGCCACAACTTGCTATTCACAACTTGTTTTAATTCATTCGGTGGGATGAAGATCTTATTCCCCAATATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTATATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAGGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAATGTTGTGTGGGTATCAGCCATTCGCGACCAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGATTCACCGGCGACGGTGAAGAGTTGCTGAAACACGTGCTATGGTCAAACGGACCAGAGACCCAGTCGCCGACGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTCTTCATCTCTCGCCTTCTCGTCGTCGAACTCTTCCTCCGGTACGACTCCTTCGACATCGAAGCCACAAACACTCCGTTAGGCGCCGCCGTCACTGTAACTTCCCTGAAGAAAGCAAGTTTCTGA

Coding sequence (CDS)

ATGTCGACTACCTCCATTATTATCCCTTCTCTTCAAACCCAGTTGCGATTCCCGTCGCAAGAAACGCCTCTGAGATCTCGTTCTAGAGTTGGGTTCGTTTCCCTACGCCCAATCTGCGCCGCCGACAGGGTTTCCTCCACGTCTTCCTCCTCTGTTCAAGTGCCGCAGCGGACTGTTTCGCCGCCGGAACCTACCAAGCTTCCTTTGAGGAAGATTCCAGGTGACTATGGGCCGCCGATGTTTGGGGCGTTGAAGGACCGACAAGACTATTTCTATAATCAGGGGAGGGAAGAGTATCTGAAATCTCGAATGCTTCGGTATGAATCCACTGTGTATAGAACCAATATGCCACCGGGTCCATTTATCACTTCCGATTCTCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGACAATTCTAAAGTTGAGAAGAAGGATCTCTTTACCGGGACTTACATGCCTTTAACAGAGCTCACCGGCGGTTACAGGGTTCTCTCTTATATTGACCCATCTGAGCCCGATCACGAGAAGCTTAAACAGCTCATCTTCTTCCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTCTCCGAGCTATTTGAGACTCTGGAGAAGGATTTGGCTGCTGCTGGTAAAGCCGAGTACAATGCTTCCGGTGAACAGGCGGCGTTTAATTTCTTGGCTCGGTCTCTCTTCGGAGCCAATCCGGTCGATACCAAATTGGGTCGTGACGCTCCGAAATTGATTTCGAAATGGGTTTTGTTCCAGCTTGGCCCTGTTCTCAGGCTCGGTCTCCCCAAGGTCGTCGAGGAGCTTCTCCTCCGCACGGTCCGGCTCCCGCCGGCGCTGATCAAAGCCGATTACCGGCGTCTGTATGACTTCTTTTACCAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGCCACAACTTGCTATTCACAACTTGTTTTAATTCATTCGGTGGGATGAAGATCTTATTCCCCAATATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCAACTCGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGGGCTATGGAACAGATGCCGCTGATGAAATCAGTGGTATATGAAGCGTTCAGAATCGAGCCGCCGGTTCCGGTTCAGTACGGTCGGGCGAAGAAGGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAATGTTGTGTGGGTATCAGCCATTCGCGACCAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTGCCTGATCGATTCACCGGCGACGGTGAAGAGTTGCTGAAACACGTGCTATGGTCAAACGGACCAGAGACCCAGTCGCCGACGGTTCAGAACAAGCAGTGCGCCGGAAAAGACTTCATCGTCTTCATCTCTCGCCTTCTCGTCGTCGAACTCTTCCTCCGGTACGACTCCTTCGACATCGAAGCCACAAACACTCCGTTAGGCGCCGCCGTCACTGTAACTTCCCTGAAGAAAGCAAGTTTCTGA

Protein sequence

MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF

Homology

[Close]

BLAST of Lsi08G014260 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.6e-209
Identity = 356/536 (66.42%), Postives = 447/536 (83.40%), Query Frame = 0


Query: 1   MSTTSIIIPSLQTQLRFPSQ-ETPLRSRSRVGFVSLRPICAA-DRVSSTSSSSVQVPQRT 60
           M++TS+ +PSL  +L+FPS   +  R  S    VS+RPI A+   +    SS  Q P  +
Sbjct: 1   MASTSLSLPSL--KLQFPSHTSSSSRKNSSSYRVSIRPIQASVSEIPPYISSPSQSPSSS 60

Query: 61  VSPP-EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMP 120
            SPP +  KLP +K+PGDYG P+ G  KDR DYFYNQG+ E+ KSR+ +++STV+RTNMP
Sbjct: 61  SSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRTNMP 120

Query: 121 PGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEP 180
           PGPFI+ +  VVVLLDGKSFPVLFD SKVEKKDLFTGT+MP T+LTGGYRVLSY+DPSEP
Sbjct: 121 PGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPSEP 180

Query: 181 DHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLA 240
           +H KLK+L+F+LL  RR++++PEFH+++SELFETLE +L+  GKA  NA+ +QAAFNFLA
Sbjct: 181 NHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAGLNAANDQAAFNFLA 240

Query: 241 RSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADY 300
           RSL+G NP DT+LG D PKLI KWVLFQL P+L LGLPKV+E+L++ T RLPPAL+K DY
Sbjct: 241 RSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKVLEDLVMHTFRLPPALVKKDY 300

Query: 301 RRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGV 360
           +RLY+FFY++S +V +EA+++GISREEACHNLLF TCFNSFGG+KI FPNM+KWIGRAG 
Sbjct: 301 QRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKWIGRAGA 360

Query: 361 NLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVE 420
            LH+QLA+EIR+ + +N GK+TM AME+MPLMKSVVYE+ RIEPPV  QYGRAK D+V+E
Sbjct: 361 KLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAKHDMVIE 420

Query: 421 SHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQ 480
           SHDA+FEIKEGE+L GYQPFAT+DPKIFDR++EFV DRF G +GE+LLKHVLWSNG ET+
Sbjct: 421 SHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWSNGSETE 480

Query: 481 SPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           + ++ NKQCAGKDF+V +SRLL+VELFLRYDSF+IE   +PLGAA+T+TSL++ASF
Sbjct: 481 NASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRASF 534

[Close]

BLAST of Lsi08G014260 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 4.3e-196
Identity = 325/503 (64.61%), Postives = 412/503 (81.91%), Query Frame = 0


Query: 34  SLRPICAADRVSSTSSSSVQVPQRTVSPP---EPTKLPLRKIPGDYGPPMFGALKDRQDY 93
           + RPI    + S    S +++P  T  PP   + T LP+R+IPGDYG P  G ++DR DY
Sbjct: 38  AFRPI----KASLFGDSPIKIPGITSQPPPSSDETTLPIRQIPGDYGLPGIGPIQDRLDY 97

Query: 94  FYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKD 153
           FYNQGREE+ KSR+ +Y+STVYR NMPPGPFI S+ RV+VLLD KSFPVLFD SKVEKKD
Sbjct: 98  FYNQGREEFFKSRLQKYKSTVYRANMPPGPFIASNPRVIVLLDAKSFPVLFDMSKVEKKD 157

Query: 154 LFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFE 213
           LFTGTYMP TELTGGYR+LSY+DPSEP+H KLKQL+F L+K+RRD ++PEF S+F++L E
Sbjct: 158 LFTGTYMPSTELTGGYRILSYLDPSEPNHTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCE 217

Query: 214 TLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVL 273
            +E DLA  GKA +N   EQAAFNFL+R+ FG  P+DT LG+DAP LISKWVLF L P+L
Sbjct: 218 VVEYDLATKGKAAFNDPAEQAAFNFLSRAFFGVKPIDTPLGKDAPSLISKWVLFNLAPIL 277

Query: 274 RLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSEAVFEEADRLGISREEACHNLL 333
            +GLPK VEE  L +VRLPP L++ DY RLY+FF  ++ +V +EA++ GISR+EACHN+L
Sbjct: 278 SVGLPKEVEEATLHSVRLPPLLVQNDYHRLYEFFTSAAGSVLDEAEQSGISRDEACHNIL 337

Query: 334 FTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRTAVKA-NGGKITMGAMEQMPLM 393
           F  CFNS+GG KILFP+++KWIGRAG+ LHT+LA+EIR+A+++  GGK+TM AMEQMPLM
Sbjct: 338 FAVCFNSWGGFKILFPSLMKWIGRAGLELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLM 397

Query: 394 KSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDPKIFDRAD 453
           KSVVYE  RIEPPV +QYG+AKKD ++ESH+AA+++KEGEML GYQPFAT+DPKIFDR +
Sbjct: 398 KSVVYETLRIEPPVALQYGKAKKDFILESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPE 457

Query: 454 EFVPDRFTGDGEELLKHVLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVELFLRYDSF 513
           EFV DRF G+G +L+++V+WSNGPET++P+V NKQCAGKDF+V  +RL VVELF RYDSF
Sbjct: 458 EFVADRFVGEGVKLMEYVMWSNGPETETPSVANKQCAGKDFVVMAARLFVVELFKRYDSF 517

Query: 514 DIEATNTPLGAAVTVTSLKKASF 533
           DIE   + LGA++T+TSLK+++F
Sbjct: 518 DIEVGTSSLGASITLTSLKRSTF 536

[Close]

BLAST of Lsi08G014260 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 7.4e-196
Identity = 331/522 (63.41%), Postives = 414/522 (79.31%), Query Frame = 0


Query: 10  SLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPPEPTKLPL 69
           +L   L  PS    + S+    + + RPI     VS +  S++++ Q       P KL  
Sbjct: 4   TLSFSLPLPSLHQKIPSK----YSTFRPII----VSLSDKSTIEITQ-------PIKLST 63

Query: 70  RKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVV 129
           R IPGDYG P  G  KDR DYFYNQG+ ++ +SR+ +Y+ST++RTNMPPGPFITS+ +V+
Sbjct: 64  RTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITSNPKVI 123

Query: 130 VLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFL 189
           VLLDGKSFPVLFD SKVEKKDLFTGT++P TELTGGYR+LSY+DPSEP+HEKLK+L+FFL
Sbjct: 124 VLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKKLMFFL 183

Query: 190 LKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTK 249
           L  RRD ++PEFH T++ELFETL+K++   G   +N+  +QAAFNFLARSLFG NPV+TK
Sbjct: 184 LSSRRDHVIPEFHETYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLARSLFGVNPVETK 243

Query: 250 LGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSE 309
           LG D P LI KW+L QL PV+ LGLPK ++++LL T RLPP L+K DY+RLYDFFY +S 
Sbjct: 244 LGTDGPALIGKWILLQLHPVITLGLPKFLDDVLLHTFRLPPILVKKDYQRLYDFFYTNSA 303

Query: 310 AVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRT 369
            +F EA++LGIS++EACHNLLF TCFNSFGGMKI FPNM+K I +AGV +HT+LA EIR+
Sbjct: 304 NLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHTRLANEIRS 363

Query: 370 AVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGE 429
            VK+ GGKITM AME+MPLMKSVVYEA R++PPV  QYGRAK+DL +ESHDA FE+K+GE
Sbjct: 364 EVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDAVFEVKKGE 423

Query: 430 MLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPTVQNKQCAGK 489
           +L GYQPFAT+DPKIFDR  EFV DRF G +GE+LLKHVLWSNGPET+SPTV NKQCAGK
Sbjct: 424 ILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTVGNKQCAGK 483

Query: 490 DFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKA 531
           DF+V +SRL V E FLRY + +++   + LG+++T+TSLKKA
Sbjct: 484 DFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

[Close]

BLAST of Lsi08G014260 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 676.4 bits (1744), Expect = 2.6e-193
Identity = 331/524 (63.17%), Postives = 409/524 (78.05%), Query Frame = 0


Query: 10  SLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPPEPTKLPL 69
           S+ T          +RS+     V  RPI    + S + +  + V  RT S      LP+
Sbjct: 3   SISTPFPISLHPKTVRSKPLKFRVLTRPI----KASGSETPDLTVATRTGS----KDLPI 62

Query: 70  RKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVV 129
           R IPG+YG P+ G +KDR DYFY+QG EE+ KSR+ +Y STVYR NMPPG FI  + +VV
Sbjct: 63  RNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVV 122

Query: 130 VLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFL 189
            LLDGKSFPVLFD  KVEKKDLFTGTYMP TELTGGYR+LSY+DPSEP HEKLK L+FFL
Sbjct: 123 ALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFL 182

Query: 190 LKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTK 249
           LK  R++I PEF +T+SELF++LEK+L+  GKA++  S +  AFNFLAR+ +G NP DTK
Sbjct: 183 LKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPADTK 242

Query: 250 LGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSE 309
           L  DAP LI+KWVLF L P+L +GLP+V+EE L+ T  LPPAL+K+DY+RLY+FF +S+ 
Sbjct: 243 LKADAPGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFFLESAG 302

Query: 310 AVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRT 369
            +  EAD+LGISREEA HNLLF TCFN++GGMKILFPNM+K IGRAG  +H +LA EIR+
Sbjct: 303 EILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRLAEEIRS 362

Query: 370 AVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGE 429
            +K+NGG++TMGA+E+M L KSVVYE  R EPPV  QYGRAKKDLV+ESHDAAF++K GE
Sbjct: 363 VIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGE 422

Query: 430 MLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPTVQNKQCAGK 489
           ML GYQP ATRDPKIFDRADEFVP+RF G +GE+LL+HVLWSNGPET++PTV NKQCAGK
Sbjct: 423 MLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGK 482

Query: 490 DFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           DF+V ++RL V+E+F RYDSFDIE   +PLG++V  +SL+KASF
Sbjct: 483 DFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKASF 518

[Close]

BLAST of Lsi08G014260 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 643.3 bits (1658), Expect = 2.5e-183
Identity = 304/471 (64.54%), Postives = 380/471 (80.68%), Query Frame = 0


Query: 63  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQ-GREEYLKSRMLRYESTVYRTNMPPGPF 122
           +P+  PLR+IPG YG P F  +KDR +YFY   GR+EY +SRM +Y+STV+R NMPPGPF
Sbjct: 2   DPSSKPLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPF 61

Query: 123 ITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEK 182
           ++S+ +V+VLLD KSFP+LFD SKVEKKDLFTGTYMP T+LTGGYRVLSY+DPSEP H +
Sbjct: 62  VSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQ 121

Query: 183 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLF 242
           LK L+FF+LK+  ++++P+F +T++ELFE LE +LA  GKA +N  GEQAAF FL R+ F
Sbjct: 122 LKNLLFFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYF 181

Query: 243 GANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLY 302
            +NP +TKLG  AP LIS WVLF L P L LGLP  ++E LL T RLP  LIK+ Y +LY
Sbjct: 182 NSNPEETKLGTSAPTLISSWVLFNLAPTLDLGLPWFLQEPLLHTFRLPAFLIKSTYNKLY 241

Query: 303 DFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHT 362
           D+F   +  V E+A++LG+ ++EA HN+LF  CFN+FGG+KILFPN +KWIG AG NLHT
Sbjct: 242 DYFQSVATPVMEQAEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENLHT 301

Query: 363 QLAREIRTAVKANG-GKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 422
           QLA EIR A+K+ G G +T+ A+EQMPL KSVVYE+ RIEPPVP QYG+AK +  +ESHD
Sbjct: 302 QLAEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHD 361

Query: 423 AAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTV 482
           A FE+K+GEML GYQPFAT+DPK+FDR +E+VPDRF GDGE LLK+V WSNGPET+SPTV
Sbjct: 362 ATFEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGDGEALLKYVWWSNGPETESPTV 421

Query: 483 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKAS 532
           +NKQCAGKDF+V I+RL V+ELF RYDSF+IE   +PLGAAVT+T LK+AS
Sbjct: 422 ENKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRAS 472

[Close]

BLAST of Lsi08G014260 vs. ExPASy TrEMBL
Match: A0A5A7VCB0 (Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G006100 PE=4 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.3e-288
Identity = 498/530 (93.96%), Postives = 519/530 (97.92%), Query Frame = 0


Query: 4   TSIIIPSLQTQLRFP-SQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFP SQETP RSRSRVGFVS+RPI AAD VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYAADGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

[Close]

BLAST of Lsi08G014260 vs. ExPASy TrEMBL
Match: A0A1S3BAD5 (allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 1.9e-287
Identity = 496/530 (93.58%), Postives = 517/530 (97.55%), Query Frame = 0


Query: 4   TSIIIPSLQTQLRFP-SQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFP SQETP RSRSRVGFVS+RPI A D VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

[Close]

BLAST of Lsi08G014260 vs. ExPASy TrEMBL
Match: U5YTX9 (Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 9.4e-287
Identity = 497/531 (93.60%), Postives = 518/531 (97.55%), Query Frame = 0


Query: 4   TSIIIPSLQTQLRFP-SQETPLRSRSRVGFVSLRPIC-AADRVSSTSSSSVQVPQRTVSP 63
           +SI+IPSLQ  LRFP SQETP RSRSRVGFVS+RPI  AAD VSS+SSSS++VPQR VSP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVSP 61

Query: 64  PEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 123
           PEP KLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 62  PEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 121

Query: 124 ITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEK 183
           ITSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH K
Sbjct: 122 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 181

Query: 184 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLF 243
           LKQL+FFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+G+AEYNA GEQAAFNFLARSLF
Sbjct: 182 LKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSLF 241

Query: 244 GANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLY 303
           GA+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 242 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 301

Query: 304 DFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHT 363
           +FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT
Sbjct: 302 EFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 361

Query: 364 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 423
           QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 421

Query: 424 AFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQ 483
           AFEIKEGEM+CGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSP+VQ
Sbjct: 422 AFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 481

Query: 484 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           NKQCAGKDFIVFISRL+VVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 NKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

[Close]

BLAST of Lsi08G014260 vs. ExPASy TrEMBL
Match: Q8L5M5 (Allene oxide synthase OS=Cucumis melo OX=3656 GN=AOS PE=2 SV=1)

HSP 1 Score: 988.8 bits (2555), Expect = 8.8e-285
Identity = 495/536 (92.35%), Postives = 516/536 (96.27%), Query Frame = 0


Query: 4   TSIIIPSLQTQLRFP-SQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFP SQETP RSRSRVGFVS+RPI A D VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLG------AAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLG      AAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

[Close]

BLAST of Lsi08G014260 vs. ExPASy TrEMBL
Match: A0A6J1K6Z3 (allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491740 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 1.7e-280
Identity = 486/536 (90.67%), Postives = 506/536 (94.40%), Query Frame = 0


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSS----SSVQVPQ 60
           MS TSI +P  Q  LRFPSQ+ PLRSRS +GF S R I AAD VSS+SS    SSVQ+PQ
Sbjct: 1   MSVTSITLPCPQPHLRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQ 60

Query: 61  RTVSPPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           R    PEPTKLPLRKIPGDYGPP+FGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 61  RIAPTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120

Query: 121 PPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSE 180
           PPGPFIT+DSRVVVLLDGKSFPVLFDN+KVEKKDLFTGTYMP TELTGGYRVLSYIDPSE
Sbjct: 121 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 180

Query: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFL 240
           PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELF+TLEKDLAA G+AEYNA GEQAAFNFL
Sbjct: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 240

Query: 241 ARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKAD 300
           ARSLFGANPVDTKLGRDAPKLI+KW+LFQLGPVLRLGLPKVVEELLLRTVRLPP+LIKAD
Sbjct: 241 ARSLFGANPVDTKLGRDAPKLIAKWILFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 300

Query: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAG 360
           YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAG
Sbjct: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360

Query: 361 VNLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420
            NLHTQLAREIRTAV+ANGG+ITMGAMEQMPLMKSVV+EAFRIEPPVPVQYGRAKKDLVV
Sbjct: 361 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVHEAFRIEPPVPVQYGRAKKDLVV 420

Query: 421 ESHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGEM+CG+QPFATRDPKIFDRADEFVPDRFTGDGE LLKHVLWSNGPETQ
Sbjct: 421 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQ 480

Query: 481 SPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           SPTVQNKQCAGKDFIVFISRLLVVE FLRYDSFDIE  N+PLGAA+TVTSLKKASF
Sbjct: 481 SPTVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: KAA0065268.1 (allene oxide synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.7e-288
Identity = 498/530 (93.96%), Postives = 519/530 (97.92%), Query Frame = 0


Query: 4   TSIIIPSLQTQLRFP-SQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFP SQETP RSRSRVGFVS+RPI AAD VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYAADGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: XP_038885277.1 (allene oxide synthase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 996.9 bits (2576), Expect = 6.7e-287
Identity = 501/534 (93.82%), Postives = 515/534 (96.44%), Query Frame = 0


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRV--SSTSSSSVQVPQRT 60
           MS+TSI IP LQ  LRFP QE P RSRSRVGFVSLRPI AADRV  SS+SSSSVQVPQRT
Sbjct: 1   MSSTSITIPCLQPPLRFPYQEPPPRSRSRVGFVSLRPIYAADRVSSSSSSSSSVQVPQRT 60

Query: 61  VSPPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPP 120
           VS PEPTKLPLRKIPGDYGPP+FGALKDRQDYFYNQG+EEYLKSRMLRYESTVYRTNMPP
Sbjct: 61  VSTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGKEEYLKSRMLRYESTVYRTNMPP 120

Query: 121 GPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPD 180
           GPFITSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGG+RVLSYIDPSEPD
Sbjct: 121 GPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGHRVLSYIDPSEPD 180

Query: 181 HEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLAR 240
           H KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAG+A+YNA GEQAAFNFLAR
Sbjct: 181 HAKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGRAQYNAPGEQAAFNFLAR 240

Query: 241 SLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYR 300
           SLFGANPVD+KLGRDAPKLI KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYR
Sbjct: 241 SLFGANPVDSKLGRDAPKLIGKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYR 300

Query: 301 RLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVN 360
           RLYDFFY+SSEAVFEEADRLGISREEACHNLLF TCFNSFGGMKI FPNMIKWIGRAGV 
Sbjct: 301 RLYDFFYESSEAVFEEADRLGISREEACHNLLFATCFNSFGGMKIFFPNMIKWIGRAGVK 360

Query: 361 LHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVES 420
           LHTQLAREIRTAVK+NGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVES
Sbjct: 361 LHTQLAREIRTAVKSNGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVES 420

Query: 421 HDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSP 480
           HDAAFEIKEGE++CGYQPFATRDPKIFDRA EFVPDRFTGDGEELLKHVLWSNGPETQSP
Sbjct: 421 HDAAFEIKEGEVICGYQPFATRDPKIFDRAGEFVPDRFTGDGEELLKHVLWSNGPETQSP 480

Query: 481 TVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           TVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF
Sbjct: 481 TVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 534

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: NP_001274390.1 (allene oxide synthase [Cucumis sativus] >AGZ95026.1 allene oxide synthase [Cucumis sativus] >KGN62561.1 hypothetical protein Csa_021833 [Cucumis sativus])

HSP 1 Score: 995.3 bits (2572), Expect = 1.9e-286
Identity = 497/531 (93.60%), Postives = 518/531 (97.55%), Query Frame = 0


Query: 4   TSIIIPSLQTQLRFP-SQETPLRSRSRVGFVSLRPIC-AADRVSSTSSSSVQVPQRTVSP 63
           +SI+IPSLQ  LRFP SQETP RSRSRVGFVS+RPI  AAD VSS+SSSS++VPQR VSP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVSP 61

Query: 64  PEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 123
           PEP KLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF
Sbjct: 62  PEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPF 121

Query: 124 ITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEK 183
           ITSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH K
Sbjct: 122 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 181

Query: 184 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLF 243
           LKQL+FFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+G+AEYNA GEQAAFNFLARSLF
Sbjct: 182 LKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSLF 241

Query: 244 GANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLY 303
           GA+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLY
Sbjct: 242 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 301

Query: 304 DFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHT 363
           +FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT
Sbjct: 302 EFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 361

Query: 364 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 423
           QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA
Sbjct: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 421

Query: 424 AFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQ 483
           AFEIKEGEM+CGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSP+VQ
Sbjct: 422 AFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 481

Query: 484 NKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           NKQCAGKDFIVFISRL+VVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 482 NKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: NP_001315375.1 (allene oxide synthase 1, chloroplastic [Cucumis melo] >AAM66138.1 allene oxide synthase [Cucumis melo])

HSP 1 Score: 988.8 bits (2555), Expect = 1.8e-284
Identity = 495/536 (92.35%), Postives = 516/536 (96.27%), Query Frame = 0


Query: 4   TSIIIPSLQTQLRFP-SQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPP 63
           +SI+IPSLQ  LRFP SQETP RSRSRVGFVS+RPI A D VSS+SSSS+QVPQR VSPP
Sbjct: 2   SSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIYATDGVSSSSSSSLQVPQRIVSPP 61

Query: 64  EPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 123
           EPTKLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI
Sbjct: 62  EPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 121

Query: 124 TSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKL 183
           TSDSRVVVLLDGKSFPVLFD+SKVEKKDLFTGTYMP+TELTGGYRVLSYIDPSEPDH KL
Sbjct: 122 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 181

Query: 184 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFG 243
           KQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAAAG+AEYNASGEQAAFNFLARSLFG
Sbjct: 182 KQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSLFG 241

Query: 244 ANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYD 303
           A+PVD+KLGRDAPKLI+KWVLFQLGPVL LGLPKVVEELLLRTVRLPPALIKADYRRLYD
Sbjct: 242 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYD 301

Query: 304 FFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQ 363
           FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAGVNLHT+
Sbjct: 302 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 361

Query: 364 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 423
           LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHDAA
Sbjct: 362 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHDAA 421

Query: 424 FEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPTVQN 483
           FEIKEGE++CGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSP+VQN
Sbjct: 422 FEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSVQN 481

Query: 484 KQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLG------AAVTVTSLKKASF 533
           KQCAGKDFIVFISRLLVVELFLRYDSFDIEA+NTPLG      AAVTVTSLKKASF
Sbjct: 482 KQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

[Close]

BLAST of Lsi08G014260 vs. NCBI nr
Match: XP_022996510.1 (allene oxide synthase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 974.5 bits (2518), Expect = 3.6e-280
Identity = 486/536 (90.67%), Postives = 506/536 (94.40%), Query Frame = 0


Query: 1   MSTTSIIIPSLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSS----SSVQVPQ 60
           MS TSI +P  Q  LRFPSQ+ PLRSRS +GF S R I AAD VSS+SS    SSVQ+PQ
Sbjct: 1   MSVTSITLPCPQPHLRFPSQKKPLRSRSNLGFPSRRLIYAADGVSSSSSSSPPSSVQLPQ 60

Query: 61  RTVSPPEPTKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           R    PEPTKLPLRKIPGDYGPP+FGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 61  RIAPTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120

Query: 121 PPGPFITSDSRVVVLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSE 180
           PPGPFIT+DSRVVVLLDGKSFPVLFDN+KVEKKDLFTGTYMP TELTGGYRVLSYIDPSE
Sbjct: 121 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 180

Query: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFL 240
           PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELF+TLEKDLAA G+AEYNA GEQAAFNFL
Sbjct: 181 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 240

Query: 241 ARSLFGANPVDTKLGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKAD 300
           ARSLFGANPVDTKLGRDAPKLI+KW+LFQLGPVLRLGLPKVVEELLLRTVRLPP+LIKAD
Sbjct: 241 ARSLFGANPVDTKLGRDAPKLIAKWILFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 300

Query: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAG 360
           YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKI FPNMIKWIGRAG
Sbjct: 301 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360

Query: 361 VNLHTQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420
            NLHTQLAREIRTAV+ANGG+ITMGAMEQMPLMKSVV+EAFRIEPPVPVQYGRAKKDLVV
Sbjct: 361 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVHEAFRIEPPVPVQYGRAKKDLVV 420

Query: 421 ESHDAAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGEM+CG+QPFATRDPKIFDRADEFVPDRFTGDGE LLKHVLWSNGPETQ
Sbjct: 421 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQ 480

Query: 481 SPTVQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           SPTVQNKQCAGKDFIVFISRLLVVE FLRYDSFDIE  N+PLGAA+TVTSLKKASF
Sbjct: 481 SPTVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

[Close]

BLAST of Lsi08G014260 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 676.4 bits (1744), Expect = 1.9e-194
Identity = 331/524 (63.17%), Postives = 409/524 (78.05%), Query Frame = 0


Query: 10  SLQTQLRFPSQETPLRSRSRVGFVSLRPICAADRVSSTSSSSVQVPQRTVSPPEPTKLPL 69
           S+ T          +RS+     V  RPI    + S + +  + V  RT S      LP+
Sbjct: 3   SISTPFPISLHPKTVRSKPLKFRVLTRPI----KASGSETPDLTVATRTGS----KDLPI 62

Query: 70  RKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVV 129
           R IPG+YG P+ G +KDR DYFY+QG EE+ KSR+ +Y STVYR NMPPG FI  + +VV
Sbjct: 63  RNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVV 122

Query: 130 VLLDGKSFPVLFDNSKVEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFL 189
            LLDGKSFPVLFD  KVEKKDLFTGTYMP TELTGGYR+LSY+DPSEP HEKLK L+FFL
Sbjct: 123 ALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFL 182

Query: 190 LKHRRDKILPEFHSTFSELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGANPVDTK 249
           LK  R++I PEF +T+SELF++LEK+L+  GKA++  S +  AFNFLAR+ +G NP DTK
Sbjct: 183 LKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPADTK 242

Query: 250 LGRDAPKLISKWVLFQLGPVLRLGLPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSSE 309
           L  DAP LI+KWVLF L P+L +GLP+V+EE L+ T  LPPAL+K+DY+RLY+FF +S+ 
Sbjct: 243 LKADAPGLITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFFLESAG 302

Query: 310 AVFEEADRLGISREEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRT 369
            +  EAD+LGISREEA HNLLF TCFN++GGMKILFPNM+K IGRAG  +H +LA EIR+
Sbjct: 303 EILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRLAEEIRS 362

Query: 370 AVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGE 429
            +K+NGG++TMGA+E+M L KSVVYE  R EPPV  QYGRAKKDLV+ESHDAAF++K GE
Sbjct: 363 VIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGE 422

Query: 430 MLCGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPTVQNKQCAGK 489
           ML GYQP ATRDPKIFDRADEFVP+RF G +GE+LL+HVLWSNGPET++PTV NKQCAGK
Sbjct: 423 MLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGK 482

Query: 490 DFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 533
           DF+V ++RL V+E+F RYDSFDIE   +PLG++V  +SL+KASF
Sbjct: 483 DFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKASF 518

[Close]

BLAST of Lsi08G014260 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 277.7 bits (709), Expect = 1.9e-74
Identity = 143/367 (38.96%), Postives = 216/367 (58.86%), Query Frame = 0


Query: 146 VEKKDLFTGTYMPLTELTGGYRVLSYIDPSEPDHEKLKQLIFFLLKHRRDKILPEFHSTF 205
           V+K+D+  G + P     GG RV  Y+D +EP H K+K      LK      L E  S  
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 206 SELFETLEKDLAAAGKAEYNASGEQAAFNFLARSLFGAN-PVDTKLGRDAPKLISKWVLF 265
           +  + T+E +++  G A Y    ++  F+FL  SL G +  V   +  +  K I+ W+  
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLAL 123

Query: 266 QLGPVLRLG-LPKVVEELLLRTVRLPPALIKADYRRLYDFFYQSS-EAVFEEADRLGISR 325
           Q+ P  +LG +P+ +EE+LL T   P  LI  +Y++LY+F  +++ + +    +  G++R
Sbjct: 124 QVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFGLTR 183

Query: 326 EEACHNLLFTTCFNSFGGMKILFPNMIKWIGRAGVNLHTQLAREIRTAVKANGGKITMGA 385
           +EA  NLLF   FN++GG  +  P++I  I      L  ++  E+R  V  +G  +    
Sbjct: 184 DEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVR-RVCGSGSDLNFKT 243

Query: 386 MEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEMLCGYQPFATRDP 445
           + +M L+KSVVYE  R  PPVP+Q+ RA+KD  + SHDA FE+K+GE+LCGYQP   RD 
Sbjct: 244 VNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDA 303

Query: 446 KIFDRADEFVPDRFTGD-GEELLKHVLWSNGPETQSPTVQNKQCAGKDFIVFISRLLVVE 505
            +FD  +EF PDR+ G+ G ELL ++ WSNGP+T +P+  NKQCA KD +   + LLV +
Sbjct: 304 NVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVAD 363

Query: 506 LFLRYDS 509
           LFLRYD+
Sbjct: 364 LFLRYDT 369

[Close]

BLAST of Lsi08G014260 vs. TAIR 10
Match: AT3G53280.1 (cytochrome p450 71b5 )

HSP 1 Score: 55.8 bits (133), Expect = 1.2e-07
Identity = 33/96 (34.38%), Postives = 52/96 (54.17%), Query Frame = 0


Query: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 421
           +L  EIR A+  N  KIT   +E++  +K V+ EAFR+ PP P+   R    D+ ++   
Sbjct: 324 KLQEEIRAALGPNKEKITEEDLEKVEYLKMVIEEAFRLHPPAPLLLPRLTMSDINIQ--- 383

Query: 422 AAFEIKEGEMLCGYQPFATRDPKIFDRADEFVPDRF 457
             + I +  M+        RDPK + + DEF+P+RF
Sbjct: 384 -GYSIPKNTMIQINTYTIGRDPKNWTKPDEFIPERF 415

[Close]

BLAST of Lsi08G014260 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 45.4 bits (106), Expect = 1.6e-04
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 421
           +L  EIR+ ++ +G  I    +E M  +K+V+ E FR+ PP+P+   R   +D+ V+ +D
Sbjct: 323 KLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD 382

Query: 422 AAFEIKEGEMLCGYQPFATRDPKIF-DRADEFVPDR 456
               I  G  +        RDP I+   A+EF P+R
Sbjct: 383 ----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPER 414

[Close]

BLAST of Lsi08G014260 vs. TAIR 10
Match: AT1G11610.2 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 45.4 bits (106), Expect = 1.6e-04
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 362 QLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 421
           +L  EIR+ ++ +G  I    +E M  +K+V+ E FR+ PP+P+   R   +D+ V+ +D
Sbjct: 323 KLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD 382

Query: 422 AAFEIKEGEMLCGYQPFATRDPKIF-DRADEFVPDR 456
               I  G  +        RDP I+   A+EF P+R
Sbjct: 383 ----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPER 414

The following BLAST results are available for this feature:

BLAST of Lsi08G014260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
K4BV52	2.6e-209	66.42	Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P...	[more]
P48417	4.3e-196	64.61	Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE...	[more]
Q9LLB0	7.4e-196	63.41	Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P...	[more]
Q96242	2.6e-193	63.17	Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P...	[more]
Q40778	2.5e-183	64.54	Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2	[more]

BLAST of Lsi08G014260 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VCB0	1.3e-288	93.96	Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A1S3BAD5	1.9e-287	93.58	allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 P...	[more]
U5YTX9	9.4e-287	93.60	Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1	[more]
Q8L5M5	8.8e-285	92.35	Allene oxide synthase OS=Cucumis melo OX=3656 GN=AOS PE=2 SV=1	[more]
A0A6J1K6Z3	1.7e-280	90.67	allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]

BLAST of Lsi08G014260 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0065268.1	2.7e-288	93.96	allene oxide synthase 1 [Cucumis melo var. makuwa]	[more]
XP_038885277.1	6.7e-287	93.82	allene oxide synthase 1, chloroplastic [Benincasa hispida]	[more]
NP_001274390.1	1.9e-286	93.60	allene oxide synthase [Cucumis sativus] >AGZ95026.1 allene oxide synthase [Cucum...	[more]
NP_001315375.1	1.8e-284	92.35	allene oxide synthase 1, chloroplastic [Cucumis melo] >AAM66138.1 allene oxide s...	[more]
XP_022996510.1	3.6e-280	90.67	allene oxide synthase 1, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Lsi08G014260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42650.1	1.9e-194	63.17	allene oxide synthase	[more]
AT4G15440.1	1.9e-74	38.96	hydroperoxide lyase 1	[more]
AT3G53280.1	1.2e-07	34.38	cytochrome p450 71b5	[more]
AT1G11610.1	1.6e-04	32.29	cytochrome P450, family 71, subfamily A, polypeptide 18	[more]
AT1G11610.2	1.6e-04	32.29	cytochrome P450, family 71, subfamily A, polypeptide 18	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 386..402 score: 31.0 coord: 485..503 score: 21.66 coord: 439..457 score: 31.17
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 361..459 e-value: 1.7E-13 score: 50.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 63..532 e-value: 1.0E-116 score: 392.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 67..507
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..72
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..60
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 48..530
None	No IPR available	PANTHER	PTHR24286:SF267	ALLENE OXIDE SYNTHASE 1, CHLOROPLASTIC-LIKE	coord: 48..530

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G014260.1	Lsi08G014260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009695	jasmonic acid biosynthetic process
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen