Lsi08G014050 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G014050 vs. ExPASy Swiss-Prot
Match: Q9FRK5 (Uncharacterized membrane protein At1g75140 OS=Arabidopsis thaliana OX=3702 GN=At1g75140 PE=1 SV=3)

HSP 1 Score: 644.4 bits (1661), Expect = 1.4e-183
Identity = 355/672 (52.83%), Postives = 456/672 (67.86%), Query Frame = 0

Query: 1   MANPRKGKLLFIYF----LFAFAFPHVSRVFVCSSSSFEQNQSETEVPF-----EIRQYY 60
           MA+ + GK  F +F    +  F  P  S V    S       S+            RQ  
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  VLKNLEELVRNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGK 120
            L  LEELVRNL+++V++L+ +LS+ P  V +E               + DE + K K  
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKVKKE--------------ITRDEIEEKAK-- 120

Query: 121 GKEDSLNDGGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPL 180
                                    A SVTKYSPFWSERF F SAVKL+S+ATCINVLP 
Sbjct: 121 -------------------------AFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPF 180

Query: 181 RDFERHSKYVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGH 240
           RD E  SKY AVGD  GR++VF+RNGDV VEF T  +SPITAM+SYMS+YKNE+ +VTGH
Sbjct: 181 RDHEGLSKYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGH 240

Query: 241 KNGAILMHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSD 300
           ++G IL+HR+ EGS GE+LNS  ME+V +F  T   ED   +++LEVHHVGR RYIL++D
Sbjct: 241 QSGVILLHRLREGSIGEDLNSAVMENVGKFDGT---EDGLQVTLLEVHHVGRVRYILATD 300

Query: 301 VRGRINVFKEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNY 360
           + G++ VF E+ T+YGSV P SRP+ FLKQRLLFLTE GAGSLDLRSMK+RESECEGLN+
Sbjct: 301 LSGKLTVFTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNH 360

Query: 361 SLARNYVFDATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKG 420
           SLAR+YVFDA+ER+KAYG+TS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+  QA+KG
Sbjct: 361 SLARSYVFDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKG 420

Query: 421 YLLVISNEKVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNS----DLESEGNFI 480
           YLL+++ EKV V+NVS+QHYVR   PRLLF A L++IR +FL+++ S    D +      
Sbjct: 421 YLLIVNQEKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVT 480

Query: 481 PLISSDREKLLVLGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKK 540
           PLI+SDREKLLV+GLG GYV  Y+  LPI K EFNTMLW+SPV FFILFLFGAW FF+KK
Sbjct: 481 PLIASDREKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKK 540

Query: 541 KEALTSWGPDDPFTATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRY 600
           KE+LT+WGPDDPF++T+ ++ +   T +   ++F ++  R+ D MDLR         RRY
Sbjct: 541 KESLTAWGPDDPFSSTTMSSSST--TTAQNSSAFSESTRRNDDHMDLR---------RRY 600

Query: 601 GSPTGY-PGGATSSFRPATTSDHISRPATVDPNYR-VASELKFR-GSPLEPPGFPKRREP 657
            SP+ Y PG AT ++R   ++D  SR      NYR  A E+K+R GS L+  GF KRRE 
Sbjct: 601 VSPSRYPPGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRES 617

BLAST of Lsi08G014050 vs. ExPASy TrEMBL
Match: A0A0A0LNK9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G361500 PE=4 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 572/654 (87.46%), Postives = 601/654 (91.90%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MA  RKG+  FI+FLF FAFPHVSRVFVCSSSS EQNQSE EVPFEI+QY +LKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLNDG 120
           RNLSDVVSKLELRLSDIPTAV+REKLNTDA R VVPEV       GK K KGKE+ LNDG
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDAFRRVVPEV-------GKEKRKGKEEGLNDG 120

Query: 121 GDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSKY 180
           GDGGLD+KIQDG RTRAVSVTKYS FWSERFHFLSAVKLE+DATCINVLPLRDFE HSKY
Sbjct: 121 GDGGLDHKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKY 180

Query: 181 VAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHR 240
           VAVGDERGRIYVFV NGDVA+E  T+  SP+TAMLSYMSIYKNETLLVTGHKNGAILMHR
Sbjct: 181 VAVGDERGRIYVFVINGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHR 240

Query: 241 IWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGRINVFK 300
           IWEGSNGE+LN IFMEHVVEFVAT S+EDES IS+LE+HHVGRTRYILSSD  G+I VF+
Sbjct: 241 IWEGSNGEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFR 300

Query: 301 EDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYVFD 360
           EDGT+YGSVMP SRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLN+SLARNYVFD
Sbjct: 301 EDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFD 360

Query: 361 ATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISNEK 420
           A ERSKAYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ +KGYLLV SNEK
Sbjct: 361 AMERSKAYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEK 420

Query: 421 VHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDREKLLVL 480
           VHVFNVSSQHYVRVGAPRLLFSAGLDEI+ SFLN QNSDL SE NFIPLISSD EKL+VL
Sbjct: 421 VHVFNVSSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVL 480

Query: 481 GLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPF 540
           GLGGGYVGMYR NLPIFKGEFNTM+WTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPF
Sbjct: 481 GLGGGYVGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPF 540

Query: 541 TATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSS 600
           TATSPTTGAP+GTGSSERASFIDTPSRSTD+MDLR  GGLRGPPRRYGSPTGYP GATSS
Sbjct: 541 TATSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSS 600

Query: 601 FRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPLFANNQVVDE 655
           FRPATT+DH SRPA VDPNYR ASELKFRGSPLEPPGFPKRREPLFANNQVV++
Sbjct: 601 FRPATTNDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ 647

BLAST of Lsi08G014050 vs. ExPASy TrEMBL
Match: A0A6J1CSU7 (uncharacterized membrane protein At1g75140-like OS=Momordica charantia OX=3673 GN=LOC111013986 PE=4 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 575/661 (86.99%), Postives = 604/661 (91.38%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQ----SETEVPFEIRQYYVLKNL 60
           MANPRKGKLL IYFLF F  PHVSRVFVCSSSSFEQNQ    SE+EVPFEIRQ  +LKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVHREKL-NTDASRPVVPEVSSLDERKGKGKGKGKED 120
           EELVRNLSDVVSKLE+RLSDIPT V R+K  NTDASR VVPEV SL        G  KED
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSL--------GGRKED 120

Query: 121 SLNDGGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFE 180
            LNDGGDGGL++KI+DG RTRAVSVTKYSPFWSERFHFLSAVKL+SDATCINVLP RDFE
Sbjct: 121 GLNDGGDGGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFE 180

Query: 181 RHSKYVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGA 240
            HSKYV VGDE+GR+YVF RNGDVAVE RT SESPI  MLSYMSIYKNETLLVTGHKNGA
Sbjct: 181 GHSKYVVVGDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGA 240

Query: 241 ILMHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGR 300
           ILMHRIWE SNGEE +S+FMEHVVEFVAT  +EDESPISILEVHHVGRTRYILSSDVRG+
Sbjct: 241 ILMHRIWERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGK 300

Query: 301 INVFKEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLAR 360
           I VF+E+GT+YGSVMP S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLAR
Sbjct: 301 IKVFRENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLAR 360

Query: 361 NYVFDATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLV 420
           NYVFDATERSKAYG+TS GDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQA+KGYLLV
Sbjct: 361 NYVFDATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLV 420

Query: 421 ISNEKVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDRE 480
           ISNEKVH+FNVSSQHY RVGAPR LFSA +DEIR SFLN QN DLESEG F+PLISSDRE
Sbjct: 421 ISNEKVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDRE 480

Query: 481 KLLVLGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWG 540
           KL+VLGLGGGYVGMYR NLPIFKGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWG
Sbjct: 481 KLVVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWG 540

Query: 541 PDDPFTATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPG 600
           PDDPFTATSPTTGAPLGTG+SER+SFIDTPSRSTDMMDLRGG GLRGPPRRYGSPTGYPG
Sbjct: 541 PDDPFTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPG 600

Query: 601 GATSSFRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPLFANNQVVDEN 657
           GAT+SFRP TTSDH SRPA VDPNYR ASELKFRGSPLEPPGFPKRREPLFANNQVVDE+
Sbjct: 601 GATNSFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDES 653

BLAST of Lsi08G014050 vs. ExPASy TrEMBL
Match: A0A6J1KBU3 (uncharacterized membrane protein At1g75140-like OS=Cucurbita maxima OX=3661 GN=LOC111491879 PE=4 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 576/669 (86.10%), Postives = 601/669 (89.84%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MANPRKGKLLFIYFLF F  PHVSR FVC SSSFE NQSETEVPFE RQY +LKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPHVSRDFVCCSSSFELNQSETEVPFETRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRE-KLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLND 120
           RNLSDVVSKLELRLS+IPTAVHR+ KLNTDASR VVPEV SL        G GKED LND
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSL--------GGGKEDGLND 120

Query: 121 GGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSK 180
           GGDG LD KIQDG R RAVSVTKYSPFWSERFHFLSAVKLESDAT INVLPLRDFE HSK
Sbjct: 121 GGDGALDTKIQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSK 180

Query: 181 YVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMH 240
           YVAVGDE GRIYVFVRNGDVAVE RTMS SPITA LSYMSIYKNETLLVTGH++GAI M 
Sbjct: 181 YVAVGDETGRIYVFVRNGDVAVELRTMSGSPITATLSYMSIYKNETLLVTGHESGAISML 240

Query: 241 RIWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGRINVF 300
           RIWEGSNGEE NSIFMEHV+EFV T  +E++SPISILEVHHVGRTRYILSSDV+G+I VF
Sbjct: 241 RIWEGSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVF 300

Query: 301 KEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYVF 360
           +EDGT+YGSVMP SRPIAFLKQRLLFLTE GAGSLDLRSMKLRESECEGLNYSLARNYVF
Sbjct: 301 REDGTVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVF 360

Query: 361 DATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISNE 420
           DATERSKAYG+TSDGDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQA+KGYLLVI +E
Sbjct: 361 DATERSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSE 420

Query: 421 KVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDREKLLV 480
           KVHVFNVSSQHYVRVGAPRLLFSA LDEIR SFLNNQN DLES+G FIPLISSDREKL+V
Sbjct: 421 KVHVFNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVV 480

Query: 481 LGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540
           LGLGGGY+GMYR NLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP
Sbjct: 481 LGLGGGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540

Query: 541 FTATSPTTGAPLGTGSSE--------------RASFIDTPSRSTDMMDLRGGGGLRGPPR 600
           FTATSPTTGAPLGTGS+E              R SFIDTPSRSTDMMDLRGG GLRGPPR
Sbjct: 541 FTATSPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPR 600

Query: 601 RYGSPTGYPGGATSSFRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPL 655
           RYGSPTGYPGG TSSFRPATTSDH SR A VDPNYR ASELKFRGSPLEPPGFPKRREPL
Sbjct: 601 RYGSPTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPL 660

BLAST of Lsi08G014050 vs. ExPASy TrEMBL
Match: A0A1S3BAX2 (uncharacterized membrane protein At1g75140-like OS=Cucumis melo OX=3656 GN=LOC103487958 PE=4 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 574/657 (87.37%), Postives = 601/657 (91.48%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MA  RKG+  FI+FLF FAFPHVSRVFVCSS+SFEQNQSE EVPFEI+QY +LKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSNSFEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLNDG 120
           RNLSDVVSKLELRLSDIPTAV+REKLN DASR VVPEV       GK KGKGKED   D 
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNIDASRRVVPEV-------GKEKGKGKED---DP 120

Query: 121 GDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSKY 180
            D GLD+KIQDG RTRAVSVTKYS FWSERFHFLSAVKLESDATC NVLPLRDFE +SKY
Sbjct: 121 NDVGLDHKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLESDATCTNVLPLRDFEGNSKY 180

Query: 181 VAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHR 240
           VAVGDERGRIYVFVRNGDVA+E  T+  SPITAMLSYMSIYKNETLLVTGHKNG ILMHR
Sbjct: 181 VAVGDERGRIYVFVRNGDVAIELPTVPGSPITAMLSYMSIYKNETLLVTGHKNGGILMHR 240

Query: 241 IWEGSNGEELNSIFMEHVVEFVATGSQEDE--SPISILEVHHVGRTRYILSSDVRGRINV 300
           IWEGSNGE+LN IFME VVEFVAT S+EDE  S IS+LE+HHVGRTRYILSSD+RG+I V
Sbjct: 241 IWEGSNGEDLNLIFMERVVEFVATDSREDESQSQISLLELHHVGRTRYILSSDLRGKIKV 300

Query: 301 FKEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYV 360
           FKEDGT+YGSVMP SRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLN+SL RNYV
Sbjct: 301 FKEDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLVRNYV 360

Query: 361 FDATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISN 420
           FDATERSKAYG+TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ +KGYLLV SN
Sbjct: 361 FDATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSN 420

Query: 421 EKVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNF-IPLISSDREKL 480
           EKVHVFNVSS HYVRVGAPRLLFSAGLDEI+ SFLN QN DLESE NF IPLISSDREKL
Sbjct: 421 EKVHVFNVSSHHYVRVGAPRLLFSAGLDEIKSSFLNYQNLDLESEANFYIPLISSDREKL 480

Query: 481 LVLGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPD 540
           +VLGLGGGYVGMYR NLPIFKGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWGPD
Sbjct: 481 VVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWGPD 540

Query: 541 DPFTATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGA 600
           DPFTATSPTTGAP+GTGSSERASFIDTPSRSTD+MDLRGGGGLRGP RRYGSPTGYPGGA
Sbjct: 541 DPFTATSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRGGGGLRGPARRYGSPTGYPGGA 600

Query: 601 TSSFRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPLFANNQVVDE 655
           TSSFRPATTSDH SRPA VDPNYR ASELKFRGSPLEPPGFPKRREPLFANNQVV++
Sbjct: 601 TSSFRPATTSDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ 647

BLAST of Lsi08G014050 vs. ExPASy TrEMBL
Match: A0A6J1HC37 (uncharacterized membrane protein At1g75140-like OS=Cucurbita moschata OX=3662 GN=LOC111462650 PE=4 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 571/669 (85.35%), Postives = 600/669 (89.69%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MANP KGKLLFIYFLF F  P+VSR FVC SSS E NQSETEVP+E RQY VLKNLEELV
Sbjct: 1   MANPWKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPYETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRE-KLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLND 120
           RNLSDVVSKLELRLS+IPTAVHR+ KLNTDASR VVPEV SL        G GKED LND
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSL--------GGGKEDGLND 120

Query: 121 GGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSK 180
           GG G LD ++QDG R RAVSVTKYSPFWSERFHFLSAVKLESDAT INVLPLRDFE HSK
Sbjct: 121 GGHGALDTRVQDGGRGRAVSVTKYSPFWSERFHFLSAVKLESDATSINVLPLRDFEGHSK 180

Query: 181 YVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMH 240
           YVAVGDE GRIYVFVRNGDVAVE RT+S SPITAMLSYMSIYKNETLLVTGH++GAI MH
Sbjct: 181 YVAVGDETGRIYVFVRNGDVAVELRTVSGSPITAMLSYMSIYKNETLLVTGHESGAISMH 240

Query: 241 RIWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGRINVF 300
           RIWEGSNGEE NSIFMEHV+EFV T  +E++SPISILEVHHVGRTRYILSSDV+G+I VF
Sbjct: 241 RIWEGSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVF 300

Query: 301 KEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYVF 360
           +EDGT+YGSVMP SRPIAFLKQRLLFLTE GAGSLDLRSMKLRESECEGLNYSLARNYVF
Sbjct: 301 REDGTVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVF 360

Query: 361 DATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISNE 420
           DATERSKAYG+TSDGDLIHV+LLGDIMNFKCRVRSK+KFELDEPL FQA+KGYLLVI +E
Sbjct: 361 DATERSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLFFQAVKGYLLVIGSE 420

Query: 421 KVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDREKLLV 480
           KVHVFNVSSQHYVRVGAPRLLFSA LDEIR SFLNNQN DLES+G FIPLISSDREKL+V
Sbjct: 421 KVHVFNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNLDLESQGKFIPLISSDREKLVV 480

Query: 481 LGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540
           LGLGGGY+GMYR NLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP
Sbjct: 481 LGLGGGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540

Query: 541 FTATSPTTGAPLGTGSSE--------------RASFIDTPSRSTDMMDLRGGGGLRGPPR 600
           FTATSPTTGAPLGTGS+E              R SFIDTPSRSTDMMDLRGG GLRGPPR
Sbjct: 541 FTATSPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPR 600

Query: 601 RYGSPTGYPGGATSSFRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPL 655
           RYGSPTGYPGG TSSFRPATTSDH SR A VDPNYR ASELKFRGSPLEPPGFPKRREPL
Sbjct: 601 RYGSPTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPL 660

BLAST of Lsi08G014050 vs. NCBI nr
Match: XP_038885478.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1159.4 bits (2998), Expect = 0.0e+00
Identity = 586/654 (89.60%), Postives = 610/654 (93.27%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MAN RKGKLLFIYFLF F+FPHVSRVFVCSSSSFE NQSE EVPFEI+QY +LKNLEELV
Sbjct: 1   MANLRKGKLLFIYFLFVFSFPHVSRVFVCSSSSFELNQSEIEVPFEIKQYNLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLNDG 120
           RNLSDVVSKLELRLSDIPTAVH++KLNTDASR VV EV  L    G  K KGKED LNDG
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVHKDKLNTDASRRVVSEVVGL----GGVKRKGKEDGLNDG 120

Query: 121 GDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSKY 180
           GDGGLDNKIQDG R RAVSVTKYSPFWSERFHFLSAVKLESDATCINVLP+RDFE HSKY
Sbjct: 121 GDGGLDNKIQDGGRMRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPIRDFEGHSKY 180

Query: 181 VAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHR 240
           VAVGDE+GRIYVFVRNGDVA+E  T+SESPITAMLSY SIYKNETLLVTGH NGAILMHR
Sbjct: 181 VAVGDEKGRIYVFVRNGDVAIELPTVSESPITAMLSYTSIYKNETLLVTGHNNGAILMHR 240

Query: 241 IWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGRINVFK 300
           IWEG NGEELNSIFMEHVVEFVAT S+ED+SPISILEVHHVGRTRYILSSD RG+I VFK
Sbjct: 241 IWEGLNGEELNSIFMEHVVEFVATDSREDDSPISILEVHHVGRTRYILSSDFRGKIKVFK 300

Query: 301 EDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYVFD 360
           EDGT+YGSV  MSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRE+ CEGLNYSLARNYVFD
Sbjct: 301 EDGTVYGSVTLMSRPIAFLKQRLLFLTESGAGSLDLRSMKLREAGCEGLNYSLARNYVFD 360

Query: 361 ATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISNEK 420
           ATERSKAYG+TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQA+KGYLLVISNEK
Sbjct: 361 ATERSKAYGFTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLVISNEK 420

Query: 421 VHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDREKLLVL 480
           VHVFNVSSQHYVRV APRLLFSAGLDEIR SFLN QN +LESEG FIPLISSD EKL+VL
Sbjct: 421 VHVFNVSSQHYVRVAAPRLLFSAGLDEIRSSFLNYQNLNLESEGKFIPLISSDHEKLVVL 480

Query: 481 GLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPF 540
           GLGGGYVGMYR NLPIFKGEFN+MLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPF
Sbjct: 481 GLGGGYVGMYRSNLPIFKGEFNSMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPF 540

Query: 541 TATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSS 600
           TATSPTTG PLGTGSSERASFIDTPSRSTDMMDLRGGGG+RGPPR YGSPTGYPGGATSS
Sbjct: 541 TATSPTTGVPLGTGSSERASFIDTPSRSTDMMDLRGGGGIRGPPRHYGSPTGYPGGATSS 600

Query: 601 FRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPLFANNQVVDE 655
           FRP TTSDH SRPA VDP+YR ASELKFRGSPLEPPGFPKRREPLFANNQVV++
Sbjct: 601 FRPPTTSDHSSRPAAVDPSYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ 650

BLAST of Lsi08G014050 vs. NCBI nr
Match: XP_011649613.2 (uncharacterized membrane protein At1g75140 [Cucumis sativus] >KAE8652103.1 hypothetical protein Csa_022548 [Cucumis sativus])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 573/654 (87.61%), Postives = 602/654 (92.05%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MA  RKG+  FI+FLF FAFPHVSRVFVCSSSS EQNQSE EVPFEI+QY +LKNLEELV
Sbjct: 1   MAIERKGRFFFIFFLFIFAFPHVSRVFVCSSSSLEQNQSEIEVPFEIKQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLNDG 120
           RNLSDVVSKLELRLSDIPTAV+REKLNTDA R VVPEV       GK K KGKE+ LNDG
Sbjct: 61  RNLSDVVSKLELRLSDIPTAVNREKLNTDAFRRVVPEV-------GKEKRKGKEEGLNDG 120

Query: 121 GDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSKY 180
           GDGGLD+KIQDG RTRAVSVTKYS FWSERFHFLSAVKLE+DATCINVLPLRDFE HSKY
Sbjct: 121 GDGGLDHKIQDGGRTRAVSVTKYSSFWSERFHFLSAVKLEADATCINVLPLRDFEGHSKY 180

Query: 181 VAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHR 240
           VAVGDERGRIYVFVRNGDVA+E  T+  SP+TAMLSYMSIYKNETLLVTGHKNGAILMHR
Sbjct: 181 VAVGDERGRIYVFVRNGDVAIELPTVPGSPVTAMLSYMSIYKNETLLVTGHKNGAILMHR 240

Query: 241 IWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGRINVFK 300
           IWEGSNGE+LN IFMEHVVEFVAT S+EDES IS+LE+HHVGRTRYILSSD  G+I VF+
Sbjct: 241 IWEGSNGEDLNLIFMEHVVEFVATNSREDESQISLLELHHVGRTRYILSSDFGGKIKVFR 300

Query: 301 EDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYVFD 360
           EDGT+YGSVMP SRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLN+SLARNYVFD
Sbjct: 301 EDGTVYGSVMPTSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNHSLARNYVFD 360

Query: 361 ATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISNEK 420
           A ERSKAYG TSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPL FQ +KGYLLV SNEK
Sbjct: 361 AMERSKAYGVTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLAFQTIKGYLLVTSNEK 420

Query: 421 VHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDREKLLVL 480
           VHVFNVSSQHYVRVGAPRLLFSAGLDEI+ SFLN QNSDL SE NFIPLISSD EKL+VL
Sbjct: 421 VHVFNVSSQHYVRVGAPRLLFSAGLDEIKSSFLNYQNSDLVSEANFIPLISSDHEKLVVL 480

Query: 481 GLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPF 540
           GLGGGYVGMYR NLPIFKGEFNTM+WTSPVLFFILFLFGAW FFAKKKEALTSWGPDDPF
Sbjct: 481 GLGGGYVGMYRSNLPIFKGEFNTMVWTSPVLFFILFLFGAWHFFAKKKEALTSWGPDDPF 540

Query: 541 TATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSS 600
           TATSPTTGAP+GTGSSERASFIDTPSRSTD+MDLR  GGLRGPPRRYGSPTGYP GATSS
Sbjct: 541 TATSPTTGAPMGTGSSERASFIDTPSRSTDVMDLRSAGGLRGPPRRYGSPTGYPAGATSS 600

Query: 601 FRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPLFANNQVVDE 655
           FRPATT+DH SRPA VDPNYR ASELKFRGSPLEPPGFPKRREPLFANNQVV++
Sbjct: 601 FRPATTNDHSSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVNQ 647

BLAST of Lsi08G014050 vs. NCBI nr
Match: XP_038885660.1 (uncharacterized membrane protein At1g75140-like [Benincasa hispida])

HSP 1 Score: 1134.4 bits (2933), Expect = 0.0e+00
Identity = 575/654 (87.92%), Postives = 597/654 (91.28%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MANPRKGKLLFIY L  FAF HVSRV VCSSSSFE NQS  EVPFE+RQY +LKNLEELV
Sbjct: 1   MANPRKGKLLFIYLLLVFAFLHVSRVVVCSSSSFELNQSGIEVPFEVRQYDLLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLNDG 120
           RNLSDVVSKLELRLSDIPT V REKLN DASR V+PE                 D+LNDG
Sbjct: 61  RNLSDVVSKLELRLSDIPTVVRREKLNPDASRRVMPE----------------GDTLNDG 120

Query: 121 GDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSKY 180
           GDGGLDNKIQDGSR RAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFE +SKY
Sbjct: 121 GDGGLDNKIQDGSRARAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFEGNSKY 180

Query: 181 VAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMHR 240
           VAVGDE+GRIYVF+R+GDVA+E  T+SESPITAMLSYMSIYKNETLLVTGHKNG ILMHR
Sbjct: 181 VAVGDEKGRIYVFMRSGDVAIELPTVSESPITAMLSYMSIYKNETLLVTGHKNGMILMHR 240

Query: 241 IWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGRINVFK 300
           IWEGSNGEELN IFMEHVVEF  T SQEDESPISILEVHHVG TRYILSSD RG I VFK
Sbjct: 241 IWEGSNGEELNLIFMEHVVEFADTDSQEDESPISILEVHHVGWTRYILSSDFRGVIKVFK 300

Query: 301 EDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYVFD 360
           EDGT+YGSVMPMSRPIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLARNYVFD
Sbjct: 301 EDGTVYGSVMPMSRPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLARNYVFD 360

Query: 361 ATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISNEK 420
           ATERSKAYG+TSDGDLIHVLLLGDIMNFKCR+RSKRKFELD PLVFQA+KGYLLVISNEK
Sbjct: 361 ATERSKAYGFTSDGDLIHVLLLGDIMNFKCRIRSKRKFELDGPLVFQAIKGYLLVISNEK 420

Query: 421 VHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDREKLLVL 480
           VH+FNVSSQHYVRVGAPRLLFSAGLDEI  SFLN QN DLESEGNFIPLISSDREKL+VL
Sbjct: 421 VHIFNVSSQHYVRVGAPRLLFSAGLDEIMSSFLNYQNLDLESEGNFIPLISSDREKLVVL 480

Query: 481 GLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDPF 540
           GLGGGYVGMYR NLP+FKGEFNTMLWTSPVLFFILFLFGAW  FAKKKEALTSWGPDD F
Sbjct: 481 GLGGGYVGMYRSNLPVFKGEFNTMLWTSPVLFFILFLFGAWHLFAKKKEALTSWGPDDSF 540

Query: 541 TATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPGGATSS 600
           TATSPTTGAPLGTGSSERASFID PSR+TDMMDLRGG GLRGPPRRYGSPTGYPGGATSS
Sbjct: 541 TATSPTTGAPLGTGSSERASFIDPPSRTTDMMDLRGGAGLRGPPRRYGSPTGYPGGATSS 600

Query: 601 FRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPLFANNQVVDE 655
           FRPATTSDH SRPA VDPNYR ASE+KFRGSPLEPPGFPKRREPLFANNQVV++
Sbjct: 601 FRPATTSDHSSRPAAVDPNYRAASEIKFRGSPLEPPGFPKRREPLFANNQVVNQ 638

BLAST of Lsi08G014050 vs. NCBI nr
Match: XP_022144258.1 (uncharacterized membrane protein At1g75140-like [Momordica charantia])

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 575/661 (86.99%), Postives = 604/661 (91.38%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQ----SETEVPFEIRQYYVLKNL 60
           MANPRKGKLL IYFLF F  PHVSRVFVCSSSSFEQNQ    SE+EVPFEIRQ  +LKNL
Sbjct: 1   MANPRKGKLLLIYFLFVFGVPHVSRVFVCSSSSFEQNQPESESESEVPFEIRQRDLLKNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVHREKL-NTDASRPVVPEVSSLDERKGKGKGKGKED 120
           EELVRNLSDVVSKLE+RLSDIPT V R+K  NTDASR VVPEV SL        G  KED
Sbjct: 61  EELVRNLSDVVSKLEMRLSDIPTEVIRDKKPNTDASRRVVPEVVSL--------GGRKED 120

Query: 121 SLNDGGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFE 180
            LNDGGDGGL++KI+DG RTRAVSVTKYSPFWSERFHFLSAVKL+SDATCINVLP RDFE
Sbjct: 121 GLNDGGDGGLESKIRDGDRTRAVSVTKYSPFWSERFHFLSAVKLDSDATCINVLPFRDFE 180

Query: 181 RHSKYVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGA 240
            HSKYV VGDE+GR+YVF RNGDVAVE RT SESPI  MLSYMSIYKNETLLVTGHKNGA
Sbjct: 181 GHSKYVVVGDEKGRVYVFTRNGDVAVELRTKSESPIMTMLSYMSIYKNETLLVTGHKNGA 240

Query: 241 ILMHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGR 300
           ILMHRIWE SNGEE +S+FMEHVVEFVAT  +EDESPISILEVHHVGRTRYILSSDVRG+
Sbjct: 241 ILMHRIWERSNGEESHSLFMEHVVEFVATDGREDESPISILEVHHVGRTRYILSSDVRGK 300

Query: 301 INVFKEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLAR 360
           I VF+E+GT+YGSVMP S+PIAFLKQRLLFLTE+GAGSLDLRSMKLRESECEGLNYSLAR
Sbjct: 301 IKVFRENGTVYGSVMPESKPIAFLKQRLLFLTESGAGSLDLRSMKLRESECEGLNYSLAR 360

Query: 361 NYVFDATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLV 420
           NYVFDATERSKAYG+TS GDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQA+KGYLLV
Sbjct: 361 NYVFDATERSKAYGFTSTGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQAIKGYLLV 420

Query: 421 ISNEKVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDRE 480
           ISNEKVH+FNVSSQHY RVGAPR LFSA +DEIR SFLN QN DLESEG F+PLISSDRE
Sbjct: 421 ISNEKVHIFNVSSQHYARVGAPRPLFSAAVDEIRSSFLNYQNLDLESEGKFVPLISSDRE 480

Query: 481 KLLVLGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWG 540
           KL+VLGLGGGYVGMYR NLPIFKGEFNTMLWTSPVLFFILFLFGAW FFAKKKEALTSWG
Sbjct: 481 KLVVLGLGGGYVGMYRSNLPIFKGEFNTMLWTSPVLFFILFLFGAWHFFAKKKEALTSWG 540

Query: 541 PDDPFTATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTGYPG 600
           PDDPFTATSPTTGAPLGTG+SER+SFIDTPSRSTDMMDLRGG GLRGPPRRYGSPTGYPG
Sbjct: 541 PDDPFTATSPTTGAPLGTGTSERSSFIDTPSRSTDMMDLRGGSGLRGPPRRYGSPTGYPG 600

Query: 601 GATSSFRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPLFANNQVVDEN 657
           GAT+SFRP TTSDH SRPA VDPNYR ASELKFRGSPLEPPGFPKRREPLFANNQVVDE+
Sbjct: 601 GATNSFRPPTTSDHNSRPAAVDPNYRAASELKFRGSPLEPPGFPKRREPLFANNQVVDES 653

BLAST of Lsi08G014050 vs. NCBI nr
Match: XP_023546241.1 (uncharacterized membrane protein At1g75140-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1128.2 bits (2917), Expect = 0.0e+00
Identity = 575/669 (85.95%), Postives = 604/669 (90.28%), Query Frame = 0

Query: 1   MANPRKGKLLFIYFLFAFAFPHVSRVFVCSSSSFEQNQSETEVPFEIRQYYVLKNLEELV 60
           MANPRKGKLLFIYFLF F  P+VSR FVC SSS E NQSETEVPFE RQY VLKNLEELV
Sbjct: 1   MANPRKGKLLFIYFLFVFGIPYVSRDFVCCSSSLELNQSETEVPFETRQYDVLKNLEELV 60

Query: 61  RNLSDVVSKLELRLSDIPTAVHRE-KLNTDASRPVVPEVSSLDERKGKGKGKGKEDSLND 120
           RNLSDVVSKLELRLS+IPTAVHR+ KLNTDASR VVPEV SL        G GK+D LND
Sbjct: 61  RNLSDVVSKLELRLSNIPTAVHRDVKLNTDASRRVVPEVVSL--------GGGKQDGLND 120

Query: 121 GGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFERHSK 180
           GGDG LD +IQDG R RAVSVTKYSPFWSERFHF+SAVKLESDAT INVLPLRDFE HSK
Sbjct: 121 GGDGDLDTRIQDGGRGRAVSVTKYSPFWSERFHFMSAVKLESDATSINVLPLRDFEGHSK 180

Query: 181 YVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAILMH 240
           YVAVGDE GRIYVFVRNGDVAVE RTMS SPITAMLSYMSIYKNETLLVTGH++GAI MH
Sbjct: 181 YVAVGDETGRIYVFVRNGDVAVELRTMSGSPITAMLSYMSIYKNETLLVTGHESGAISMH 240

Query: 241 RIWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRGRINVF 300
           RIWEGSNGEE NSIFMEHV+EFV T  +E++SPISILEVHHVGRTRYILSSDV+G+I VF
Sbjct: 241 RIWEGSNGEESNSIFMEHVLEFVVTDGREEKSPISILEVHHVGRTRYILSSDVKGKIKVF 300

Query: 301 KEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLARNYVF 360
           +EDGT+YGSVMP SRPIAFLKQRLLFLTE GAGSLDLRSMKLRESECEGLNYSLARNYVF
Sbjct: 301 REDGTVYGSVMPTSRPIAFLKQRLLFLTETGAGSLDLRSMKLRESECEGLNYSLARNYVF 360

Query: 361 DATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLLVISNE 420
           DATERSKAYG+TSDGDLIHV+LLGDIMNFKCRVRSK+KFELDEPL+FQA+KGYLLVI +E
Sbjct: 361 DATERSKAYGFTSDGDLIHVMLLGDIMNFKCRVRSKKKFELDEPLLFQAVKGYLLVIGSE 420

Query: 421 KVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEGNFIPLISSDREKLLV 480
           KVHVFNVSSQHYVRVGAPRLLFSA LDEIR SFLNNQNSDLES+G FIPLISSDREKL+V
Sbjct: 421 KVHVFNVSSQHYVRVGAPRLLFSARLDEIRSSFLNNQNSDLESQGKFIPLISSDREKLVV 480

Query: 481 LGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540
           LGLGGGY+GMYR NLPI+KGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP
Sbjct: 481 LGLGGGYIGMYRSNLPIYKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALTSWGPDDP 540

Query: 541 FTATSPTTGAPLGTGSSE--------------RASFIDTPSRSTDMMDLRGGGGLRGPPR 600
           FTATSPTTGAPLGTGS+E              R SFIDTPSRSTDMMDLRGG GLRGPPR
Sbjct: 541 FTATSPTTGAPLGTGSTERTSFIDTPSRSTDMRTSFIDTPSRSTDMMDLRGGSGLRGPPR 600

Query: 601 RYGSPTGYPGGATSSFRPATTSDHISRPATVDPNYRVASELKFRGSPLEPPGFPKRREPL 655
           RYGSPTGYPGG TSSFRPATTSDH SR A VDPNYR ASELKFRGSPLEPPGFPKRREPL
Sbjct: 601 RYGSPTGYPGGVTSSFRPATTSDHSSRQAAVDPNYRAASELKFRGSPLEPPGFPKRREPL 660

BLAST of Lsi08G014050 vs. TAIR 10
Match: AT1G75140.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G19370.1); Has 51 Blast hits to 49 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 46; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 644.4 bits (1661), Expect = 9.7e-185
Identity = 355/672 (52.83%), Postives = 456/672 (67.86%), Query Frame = 0

Query: 1   MANPRKGKLLFIYF----LFAFAFPHVSRVFVCSSSSFEQNQSETEVPF-----EIRQYY 60
           MA+ + GK  F +F    +  F  P  S V    S       S+            RQ  
Sbjct: 1   MADSQNGKSAFFFFFVSLILLFLSPSYSDVTASESDPIPYENSDASPGVVTSSESDRQGV 60

Query: 61  VLKNLEELVRNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGK 120
            L  LEELVRNL+++V++L+ +LS+ P  V +E               + DE + K K  
Sbjct: 61  SLHRLEELVRNLTELVARLDAKLSETPFKVKKE--------------ITRDEIEEKAK-- 120

Query: 121 GKEDSLNDGGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPL 180
                                    A SVTKYSPFWSERF F SAVKL+S+ATCINVLP 
Sbjct: 121 -------------------------AFSVTKYSPFWSERFEFTSAVKLDSEATCINVLPF 180

Query: 181 RDFERHSKYVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGH 240
           RD E  SKY AVGD  GR++VF+RNGDV VEF T  +SPITAM+SYMS+YKNE+ +VTGH
Sbjct: 181 RDHEGLSKYFAVGDSSGRVFVFLRNGDVLVEFFTTCDSPITAMVSYMSVYKNESFVVTGH 240

Query: 241 KNGAILMHRIWEGSNGEELNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSD 300
           ++G IL+HR+ EGS GE+LNS  ME+V +F  T   ED   +++LEVHHVGR RYIL++D
Sbjct: 241 QSGVILLHRLREGSIGEDLNSAVMENVGKFDGT---EDGLQVTLLEVHHVGRVRYILATD 300

Query: 301 VRGRINVFKEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNY 360
           + G++ VF E+ T+YGSV P SRP+ FLKQRLLFLTE GAGSLDLRSMK+RESECEGLN+
Sbjct: 301 LSGKLTVFTENRTVYGSVSPTSRPLVFLKQRLLFLTETGAGSLDLRSMKIRESECEGLNH 360

Query: 361 SLARNYVFDATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKG 420
           SLAR+YVFDA+ER+KAYG+TS+G++IHVLLLGDIMNFKCRVRSK+K +++EP+  QA+KG
Sbjct: 361 SLARSYVFDASERAKAYGFTSEGEIIHVLLLGDIMNFKCRVRSKKKVQMEEPVALQAIKG 420

Query: 421 YLLVISNEKVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNS----DLESEGNFI 480
           YLL+++ EKV V+NVS+QHYVR   PRLLF A L++IR +FL+++ S    D +      
Sbjct: 421 YLLIVNQEKVFVYNVSTQHYVRTTGPRLLFPAALEDIRSTFLSHRESTKTTDHQKLEKVT 480

Query: 481 PLISSDREKLLVLGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKK 540
           PLI+SDREKLLV+GLG GYV  Y+  LPI K EFNTMLW+SPV FFILFLFGAW FF+KK
Sbjct: 481 PLIASDREKLLVMGLGDGYVATYKSKLPISKAEFNTMLWSSPVFFFILFLFGAWHFFSKK 540

Query: 541 KEALTSWGPDDPFTATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRY 600
           KE+LT+WGPDDPF++T+ ++ +   T +   ++F ++  R+ D MDLR         RRY
Sbjct: 541 KESLTAWGPDDPFSSTTMSSSST--TTAQNSSAFSESTRRNDDHMDLR---------RRY 600

Query: 601 GSPTGY-PGGATSSFRPATTSDHISRPATVDPNYR-VASELKFR-GSPLEPPGFPKRREP 657
            SP+ Y PG AT ++R   ++D  SR      NYR  A E+K+R GS L+  GF KRRE 
Sbjct: 601 VSPSRYPPGAATGAYRSVGSNDPSSRAPVETTNYRTTAQEMKYRGGSGLDSGGFGKRRES 617

BLAST of Lsi08G014050 vs. TAIR 10
Match: AT1G19370.1 (unknown protein; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G75140.1); Has 45 Blast hits to 43 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 638.6 bits (1646), Expect = 5.3e-183
Identity = 352/668 (52.69%), Postives = 450/668 (67.37%), Query Frame = 0

Query: 1   MANPRKGKLLFIYF--LFAFAFPHVSRVFVCSSSSFEQNQSETEVPFE--IRQYYVLKNL 60
           MA+P  GK  FI F  LF+F    +S ++   S   E   ++  V      R+  +L NL
Sbjct: 1   MADPLNGKSFFICFSLLFSFTLLFISPLYATESPVIEDVSTDVAVSVSETNREAVLLHNL 60

Query: 61  EELVRNLSDVVSKLELRLSDIPTAVHREKLNTDASRPVVPEVSSLDERKGKGKGKGKEDS 120
           EELV+NL+++V+ L+ +LS  P     E               S+D+  G+ K +G    
Sbjct: 61  EELVKNLTELVANLDAKLSATPLKEKNE--------------ISVDDDIGEEKERG---- 120

Query: 121 LNDGGDGGLDNKIQDGSRTRAVSVTKYSPFWSERFHFLSAVKLESDATCINVLPLRDFER 180
                            R +A SVTKYSPFWSERF F SAVKL SDATCINVLP RDFE 
Sbjct: 121 -----------------RAKAFSVTKYSPFWSERFQFTSAVKLNSDATCINVLPFRDFEG 180

Query: 181 HSKYVAVGDERGRIYVFVRNGDVAVEFRTMSESPITAMLSYMSIYKNETLLVTGHKNGAI 240
            SKY A+GD +GR+YVF+RNGDV +EF T  +SP+TAM+SY S++KN + +VTGH+NGA+
Sbjct: 181 SSKYFAIGDSKGRVYVFLRNGDVLIEFFTTVDSPVTAMVSYSSVFKNSSFVVTGHQNGAV 240

Query: 241 LMHRIWEGSNGEE--LNSIFMEHVVEFVATGSQEDESPISILEVHHVGRTRYILSSDVRG 300
           L+HRI EGSNGE+   NS+ MEHV +F    S +   P+++LEVHHVGR RYIL++D+ G
Sbjct: 241 LLHRIHEGSNGEDWNSNSVSMEHVGKFDVDDSAD---PVTLLEVHHVGRVRYILATDLSG 300

Query: 301 RINVFKEDGTIYGSVMPMSRPIAFLKQRLLFLTENGAGSLDLRSMKLRESECEGLNYSLA 360
           ++ V  E+ T+YGSV+P SRP+ FLKQRLLFLTE+GAGSLDLRSMK+RE+ECEGLN+SLA
Sbjct: 301 KLTVLTENRTVYGSVIPSSRPLVFLKQRLLFLTESGAGSLDLRSMKIRETECEGLNHSLA 360

Query: 361 RNYVFDATERSKAYGYTSDGDLIHVLLLGDIMNFKCRVRSKRKFELDEPLVFQALKGYLL 420
           R YVFDA ERSKAYG+TS+G++IHVLL GDIMNFKCRVRSK+KF+++EP+  Q++KGYLL
Sbjct: 361 RTYVFDAAERSKAYGFTSEGEIIHVLLHGDIMNFKCRVRSKKKFQMEEPVALQSIKGYLL 420

Query: 421 VISNEKVHVFNVSSQHYVRVGAPRLLFSAGLDEIRLSFLNNQNSDLESEG--NFIPLISS 480
           VI+ EKV  FNVS+QHYVR   PRLLFSAGL+EIR +FL+++ S   +       PLI+S
Sbjct: 421 VINEEKVFAFNVSTQHYVRTAGPRLLFSAGLEEIRSAFLSHRESSSRTTTVVKTRPLIAS 480

Query: 481 DREKLLVLGLGGGYVGMYRPNLPIFKGEFNTMLWTSPVLFFILFLFGAWQFFAKKKEALT 540
           DRE LLV+GL  GY  +Y+  LP  KG+FNTMLW+SPV FFILFLFGAW FFAKKKE+LT
Sbjct: 481 DRENLLVIGLENGYFAVYKSKLPTLKGDFNTMLWSSPVFFFILFLFGAWHFFAKKKESLT 540

Query: 541 SWGPDDPFTATSPTTGAPLGTGSSERASFIDTPSRSTDMMDLRGGGGLRGPPRRYGSPTG 600
           +WGPDDPFT     TGA     S++  +F +   R+ D+MDLR         RRY     
Sbjct: 541 AWGPDDPFT----PTGAQ--NSSAKEPTFTEPSRRNDDLMDLR---------RRY----- 600

Query: 601 YPGGATSSFRPATTSDHISRPATVDPNYRVASE----LKFRGSPLEPPGFPKRREPLFAN 657
               A  S+R    +D  SR A VD NYR  ++     +  GS L+  GF  RR+ LF N
Sbjct: 601 ----AGGSYRSVGANDPSSR-APVDGNYRTTAQDHNNYRGGGSGLDSNGFGNRRDHLFGN 605