Lsi08G013280 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G013280
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6
Location	chr08: 21578485 .. 21584365 (+)
RNA-Seq Expression	Lsi08G013280
Synteny	Lsi08G013280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCAACGGTTAAACCCTCTCGTTCTCAGTTTTGTATTTCGTTGTCCTTCTCCAACCGTCACTCTCTTCATAAACCCTAAAAATCACAGAAAACCCATAGCAATTTCCCACTCTTTTGATTATCTACAGTTTATGCGTTCTTAACTTATTCCTCTTCTTAGTATAGTTTCAAGATGTCTTCGAGTATTCTTCAATCTGAATCGCCTCGAAACCCTAGACTGACGTGGGAAGGCTGCAGTGTTTTGCTCGACATCAATGATGGCGACCGCTTGGTTTTTGCTCGACTCTCGCCCGCCTCGTATGTCTCAAGTTTGAGTGTTGATTTATGGTTTTCAATGCTCAGTAAGTATGATGTATGATAACGAAAATTACTTTGATTTGCAGTACGCTGAAAATTGGGAACAAAAGTTTCTCTCTAAAGCCTTTAATCGGTTGTCCATTCGGTTCCTTGTTTCAAATTGAGAATGGAGGCAATGGTCCACATCTGGCTCGCGTTATACCCTCCAGAGAAGAAGGTTTTTATTTCTTACTATGGATTTTGTTAACGAATTGGTTTCATCTTTGCAAAAAAAAAAAAAAAAAATTAACTGAAGCGATCTTCTATTTCGGTATCTTTTTCCGAAGGCATTAACTGTCAGGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATCGTAGACAATAATAAGGCACAGAGCCTCTCTAGTGAAGATATCGATGCAATGCGAAGGTAAGATATTGCTTTGCTTTTCTTTGCCACTGCTTCAAGAGGAGGCTCAAAGTTTTATCTTGAGCTTACACCTCATTTAAATTATGAAAGGCTAAAAGCTCCTACAGAGGCGTACTCAGACTCATAGTCTTTAGACTTCATGTAATTGTTAGAACATTACTGCTCTTTTAGCTACATTATAAATTTTGGTGATTTAGCCACTATTAACTTTACCGCCCAGCGTTATAAAAACGTAGAAGACAGGAAATATTAGTCATAACAATGACAATCCTGAAAGTAACTCTTATCTAAAAAAAGACAATGCTGAATATAACCATTGAGAACTTTTATTTTGTTAAAAGAATAATTAATAGTGGTGTTTTTTTTTTTCCTTTTTTTTTTTTCTTTATTGGTAATTCTTACAATATAATCGGTGAATCTTATAATTTGTTTTATCCTGTCCCTCATACTGCCTTGAGAGGCACCATTGGCTTTATCCTTTTAAGATATGGGTCCAATCACATTTTTTTTTTTACTACGAATGAAGTCACATAGTCTTGAATTAACCAGCGATGAAATGCACTGGCAATTTCAGATCCTATTCCTATCCCGTATATATTGGGCATTATTATTATTATTATTTGAATTAGTTGGTTTTGTAATAGGAGGGACCAAATATTGTTCTTTTGTTGGCCTAACGTTGCAGCACAATGAACTTGCTTGAACCCTGCCTTTTTCACTGTGATTCTAGCTCTGTGGGATCCCACTTGCAGGATGCCAACCATTTGGGACCACTGTACTAGGATTGCAGTGGCACGGGGTCTGTACCTCACATTCTCCTCATTGTTTTGACATTTATGGGAATTAATCTTCTTTGATTAATTTCATATAACTTGCTGTTGTCTTTTCTTAAATATGTTAGTATACATGATGCATACTTTCTCTCTTTATATGTAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGCGCAACATTTGAAAAGAAAACAGTTTTCTCTCAGGTGATCAGAATGTGCACACCTGAGTAGCTTTATCTTTGCATGTCCAGTATTTTTTTTTCCTGAAGTCCATATCTTATCTGCAGGAGAAATATAGGCTCAGGAAACAAAAGAAGTATGCGCCTAGGGTACTTCTGAGGAGACCTTTTACTCGAAGGTATTCATAATAACACTTGTTAACAAGAACTTTTCATAATATGTAGACTTCAAAAATAAACGCAGGGTAAATCACAATCATGAATATTTGTATTTGTAACGTCATTAGAATGCATATATTTAATGATGCTGATGTTTTTCCCCCATCTTACAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATCGGGTAATTTATTTGTCCACCTTTTCACTACATTTTTTTACCATTCAGTTTTAATTCTTTCCACATCTTGTTTTTTCAATAATTTATTTTAATTGTAACGATAGCTGCTTGTTTTCTATTCATTAGATGACTAAGTGATAATGCCGTATGAGGGCTTCAATTATTTGTTTTCTATTCAATACTTTTCTGATTTTTTCCACCAGCTACTTGTATAAGGAATAGTTTGATTTTATTTTTTCCAGTAATATCACAATTCAGAACGCAATGTGCCAGTTCAATTATTATGTTTTATTGGTAACAACATTTTTTTTGCCCTTGCCAATTATTTCTTTCTTCGTCTCCTGGAAAATCGTGTGATAATTGAATTTCACATTATTGAACATATTTGAAGTTTCTATCCTTTCCTTTTGTCATCATGCATCATGAGCTCGCTGATGGTTTGAAGTTGGAAACAAGATTAATGTACTCTGTTTCCTTGCTTTTGATTCAGATTTTTGCGATTGGATGCTTTATCTCTTCTTCTCTCTTTTGCCAACGTTACTGCAAATTCCGATGTCCTTGTGGTTGATATGGTTGGTGGTCTTCTCACTGGAGCCGTGGCTGAGCGCATAGGAGGTCTTTTTTCTCTTATTCTGGGCATTATTTAAAAATAAATAAATAAATAAAGTGGGTTCATTTTGTTTAAGAAGTGGCTGTAAATCCTTGATGAATATGAGCCATGGTATGAAGCTAGTTTTATGTGTAGTGAAAAAACATTGGGTATAAACCAGGCAGGCTCAAAATTACTTGAGCAAATGTTGACAGCCTCTTCTTTGTACTTGTCTCGAGCAAATTAAGGTCTTATAGTATAGCTAATGCGTTATGCATACAGTGTCTATGTCGTTAATCGTATTTTCTAGCCTTACAATTTCTCCAATTTAATTTTGATTTGTATTAGTGTACAAAACAAGGTTAGGTGTCCATTCAAGATGCTCTGGTTGTGAGGTAAGGGCTTACAATATTTGGAGATACCTTCAAGGTCGCGAGTATATTACTGTGTGGAAAACTTAATGGCTTAAGAACCATGTTGTTTCATGGAGTTTGGACAAAGACTCTCACCATTAGTCCAAGGAGGTCCAGTGTGTTCAAGTCCCTGCAATATTATTTCCTTCCCAATAAATATTGATTTCCACTTGTTGGGTCTTATTCATATTTCAAGTCCATGAGTGAGAGCGAGTGTTAGATGATATGATATTAAATTTGTCTTCACCTATCAACTTAAGCTTTTGGGTCAATCTGTGATTTAAGAGGGATTGAAATATGAAGAAGACCCAACAAGTAGAAATCAATATTAATCGGAAAAGAAATAACAATGTAGGGGTTTGAACACATGACCTCTTTGGACCGCCTGCTCTGATACCACCTTAAATCGTCGACTGACCCAAAAGCTTAAGTTGATGAGTGAAGGCAACTTTAATATTGTATCATCTAACATGGTCCATAGGTGTAATTCAAATAAATAGGGGGAAAAACATTGTCGGGTGTCAAATTTCAATATTATAATTACAATTTTTTGTTCTGCTTCATATTATCTGATGCTTAAATTGTGTGTTTTACAGGCAAGGGTTCTGTTTGCAATACATACGTTGGAAATAATCCAAACCCCATGGATATAGTAAGAATATTCAACTTTGATGATGAAAATTGCTCGAGGTTTGCTGCTCTAGTCCTCTTTCTTTTGTCTTCTTTAGATTACAGAATCTTGTTTTTTTTTCTTTTATTTATTATTATTTTTAAATGTCATTCTAGTTTCTACTCCTCAGTAACTCTTTATCCACCAGGATAATGCGTTCACCTCTTGCTGAGCTCGCCTCATCCCAAGTTGAAGCTTCCAAGCCTAATGGTGAACAGGAAGTTGGTGTAAGTAGAGTTTTCCTTTTCTTAATGTTTTCTTAAGATGAGTTTGAAACTGACTTCTGGAATGCTGGAGAGTACTCTTGACTAAGAGCTTAATCAAATACTTTTAAAAGGACATTATAAATATCTCTAGAAGAATTGCTTAATTTAGTCCTTCCAGAAGTACCAGTTGAAATTTGTTACTCTCAAAATTCATCTCAGGAGCAGGCATCTCAACCTCTTAGTATGGATGAAAGCCCTCTACCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATTGTTTCTCCATTAACAAAAGTATTTAAATGCGCCAAGGCTGGAGATAAGGCACCGCCAGAAGTTATTAACTTATGGAAGGAAAATGGTTTTACTAGGTGAGTAATGAGTTTTGGATGGTGGTAAAATGTGCTTCACTATTAATTATAATTCACTAAATTATTTTGATTAATTGCAGCCTAATAATTGCAGCACCACAGTCGGATGCATGGAGTCTTGTTAAAGATCTGTTGCCTCTTGTAGCAAACTCTGCCCCATTTGCTATTTACCACCAGTATCTTCAGGTTGGTTCTTCGAAAGGAAAAAAAAAAATATATATATATATATATTGGAAAAAGTTCCTTAAAATTTACCTCCTCAAGGGAGTGCTCGAAGGGCTTGATGCATTTGAACTTCATAATTGAATATGCTTATTACTTCTTGGAAGTGATTTATAGCAAGGAAAGGGGATGAACCATGAACGTAATGGTAGGAGCGGAGCTCGACACCCTGGTTATAAATTGTTTTTTTTTATAGAAATGTGATATCATGTAACGGGGAAGTGATTGTATATCAATGTTATATTTATCTATGTATCAGATTGACTAAGTTGCTTATTTGTTGAATGCAGCCTCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATTTCAGAACCCTGGCTTCGTGAATACCAGGTTTGATGGCTCTACATTTCCGCATACTTATTGCTCTAGGAGATAACGTGCAGTGTTAAGAGAAGGATTGGGGAACAATAATCTGAATTTAAACGATGGATTTATGCATATTCTTAGTCCTAGAAAAAAGTTATAAATTCAAGATAATTACACAGTTTGGATTCTATTAGTTAAACTTCATGGAGTTTACATTTGTTTGATAGCACAATGAAAAGAAAATGTCTTAACTAGCGTGCTTTGGCTTACTGGTAGTTGGTGAAAGTGAGAAAAGATAAAAAGGCTTTTGGGAAAAGGTTCAAAAATCAAATTCCATGTGGGATCCACCTAGGATCTTAGAATCTTATGAAGTCTCTAACAACCAAGAGTTATTGGGTCAATCGAGGTGATTACAAAAAATTAAGTTACTATAAAATAGGTGACAATTTTGTCAACATAATCATAACTCAGTAGAAAGGCCGAAGGTTTAAATCTTCATACTACGTTTGTTGAACTAAAAAATGACGATATTTATCATAATAATGCAGGTTCTTCCTTCGAGAACACATCCCTTCATGCAAATGAATGCGTTTGGTGGATACATTCTTAGTGGGACTAAGATATGCAACCATCAATAACACTTCCAATGTGTAGGTTTTACAGTGCAACTCTAAAATGCAGTGGAGGTCAAAACCAAATTTTGCCATGGGCTTGTATTTTCAGCTTCTGTTAATTAATTATGTATCTTTCCATTTTTATTTAATCCCTAGGATTACAGCTTAATAAACTTCCTTTTTCTTTTTATTTATTTCACATAAAACCATGTATCAATTTTATTATTGTTATTATTGAAGGTGGATCAAGGTGTGATATCTCTGCATGATTTCAATCGTCTCCAATTCAATTCGAT

mRNA sequence

ACCAACGGTTAAACCCTCTCGTTCTCAGTTTTGTATTTCGTTGTCCTTCTCCAACCGTCACTCTCTTCATAAACCCTAAAAATCACAGAAAACCCATAGCAATTTCCCACTCTTTTGATTATCTACAGTTTATGCGTTCTTAACTTATTCCTCTTCTTAGTATAGTTTCAAGATGTCTTCGAGTATTCTTCAATCTGAATCGCCTCGAAACCCTAGACTGACGTGGGAAGGCTGCAGTGTTTTGCTCGACATCAATGATGGCGACCGCTTGGTTTTTGCTCGACTCTCGCCCGCCTCTACGCTGAAAATTGGGAACAAAAGTTTCTCTCTAAAGCCTTTAATCGGTTGTCCATTCGGTTCCTTGTTTCAAATTGAGAATGGAGGCAATGGTCCACATCTGGCTCGCGTTATACCCTCCAGAGAAGAAGGCATTAACTGTCAGGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATCGTAGACAATAATAAGGCACAGAGCCTCTCTAGTGAAGATATCGATGCAATGCGAAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGCGCAACATTTGAAAAGAAAACAGTTTTCTCTCAGGAGAAATATAGGCTCAGGAAACAAAAGAAGTATGCGCCTAGGGTACTTCTGAGGAGACCTTTTACTCGAAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATCGGATTTTTGCGATTGGATGCTTTATCTCTTCTTCTCTCTTTTGCCAACGTTACTGCAAATTCCGATGTCCTTGTGGTTGATATGGTTGGTGGTCTTCTCACTGGAGCCGTGGCTGAGCGCATAGGAGGCAAGGGTTCTGTTTGCAATACATACGTTGGAAATAATCCAAACCCCATGGATATAGTAAGAATATTCAACTTTGATGATGAAAATTGCTCGAGGATAATGCGTTCACCTCTTGCTGAGCTCGCCTCATCCCAAGTTGAAGCTTCCAAGCCTAATGGTGAACAGGAAGTTGGTGAGCAGGCATCTCAACCTCTTAGTATGGATGAAAGCCCTCTACCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATTGTTTCTCCATTAACAAAAGTATTTAAATGCGCCAAGGCTGGAGATAAGGCACCGCCAGAAGTTATTAACTTATGGAAGGAAAATGGTTTTACTAGCCTAATAATTGCAGCACCACAGTCGGATGCATGGAGTCTTGTTAAAGATCTGTTGCCTCTTGTAGCAAACTCTGCCCCATTTGCTATTTACCACCAGTATCTTCAGCCTCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATTTCAGAACCCTGGCTTCGTGAATACCAGGTTCTTCCTTCGAGAACACATCCCTTCATGCAAATGAATGCGTTTGGTGGATACATTCTTAGTGGGACTAAGATATGCAACCATCAATAACACTTCCAATGTGTAGGTTTTACAGTGCAACTCTAAAATGCAGTGGAGGTCAAAACCAAATTTTGCCATGGGCTTGTATTTTCAGCTTCTGTTAATTAATTATGTATCTTTCCATTTTTATTTAATCCCTAGGATTACAGCTTAATAAACTTCCTTTTTCTTTTTATTTATTTCACATAAAACCATGTATCAATTTTATTATTGTTATTATTGAAGGTGGATCAAGGTGTGATATCTCTGCATGATTTCAATCGTCTCCAATTCAATTCGAT

Coding sequence (CDS)

ATGTCTTCGAGTATTCTTCAATCTGAATCGCCTCGAAACCCTAGACTGACGTGGGAAGGCTGCAGTGTTTTGCTCGACATCAATGATGGCGACCGCTTGGTTTTTGCTCGACTCTCGCCCGCCTCTACGCTGAAAATTGGGAACAAAAGTTTCTCTCTAAAGCCTTTAATCGGTTGTCCATTCGGTTCCTTGTTTCAAATTGAGAATGGAGGCAATGGTCCACATCTGGCTCGCGTTATACCCTCCAGAGAAGAAGGCATTAACTGTCAGGAAACGGAGGAGATGAAGGAAGAGCAGGTGACAAAAGATGAGCTGAGAGATAACAGAGCGATCGTAGACAATAATAAGGCACAGAGCCTCTCTAGTGAAGATATCGATGCAATGCGAAGTCAGGGTGCAACTGGTGATGAAATTGTGGAAGCTCTCATTGCCAACAGCGCAACATTTGAAAAGAAAACAGTTTTCTCTCAGGAGAAATATAGGCTCAGGAAACAAAAGAAGTATGCGCCTAGGGTACTTCTGAGGAGACCTTTTACTCGAAGTATATGTGAGGCATACTTCAAGAAATATCCAGCTAGAATCGGATTTTTGCGATTGGATGCTTTATCTCTTCTTCTCTCTTTTGCCAACGTTACTGCAAATTCCGATGTCCTTGTGGTTGATATGGTTGGTGGTCTTCTCACTGGAGCCGTGGCTGAGCGCATAGGAGGCAAGGGTTCTGTTTGCAATACATACGTTGGAAATAATCCAAACCCCATGGATATAGTAAGAATATTCAACTTTGATGATGAAAATTGCTCGAGGATAATGCGTTCACCTCTTGCTGAGCTCGCCTCATCCCAAGTTGAAGCTTCCAAGCCTAATGGTGAACAGGAAGTTGGTGAGCAGGCATCTCAACCTCTTAGTATGGATGAAAGCCCTCTACCATCTACAGACCAGTCCTCAGATCTCAACTCAGAGTCTATTGTTTCTCCATTAACAAAAGTATTTAAATGCGCCAAGGCTGGAGATAAGGCACCGCCAGAAGTTATTAACTTATGGAAGGAAAATGGTTTTACTAGCCTAATAATTGCAGCACCACAGTCGGATGCATGGAGTCTTGTTAAAGATCTGTTGCCTCTTGTAGCAAACTCTGCCCCATTTGCTATTTACCACCAGTATCTTCAGCCTCTTGCAACTTGCATGCATAAACTACAGCTTGAGAAGATGGCAATCAGCTTGCAAATTTCAGAACCCTGGCTTCGTGAATACCAGGTTCTTCCTTCGAGAACACATCCCTTCATGCAAATGAATGCGTTTGGTGGATACATTCTTAGTGGGACTAAGATATGCAACCATCAATAA

Protein sequence

MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSVCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVGEQASQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTHPFMQMNAFGGYILSGTKICNHQ

Homology

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BLAST of Lsi08G013280 vs. ExPASy Swiss-Prot
Match: Q54UB1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 OS=Dictyostelium discoideum OX=44689 GN=trmt6 PE=3 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.6e-53
Identity = 146/443 (32.96%), Postives = 225/443 (50.79%), Query Frame = 0


Query: 19  EGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQIENGGNGPHLAR 78
           EG  V+LDIN+G++    ++   S +KIG K   +  +IG  + S FQ+ N  N      
Sbjct: 37  EGDHVILDINNGEKFSVIKVKLGSKVKIGKKQILINSIIGESYYSSFQVSNEKN---TLE 96

Query: 79  VIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEI 138
            I  +E         E+ +         DNR +  NN AQ L+ EDI+ M+ +G   + I
Sbjct: 97  RITQKEIDDRLNNLVELNQNDA------DNRNLDQNNTAQKLTQEDINEMKQKGTDSNTI 156

Query: 139 VEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLR 198
           ++ ++ NS +F+ KT FSQ KY  +K KKY+  V + +P  +S+ EAY+KK   +I  LR
Sbjct: 157 IKTIVENSESFKTKTSFSQIKYLKKKIKKYSTIVKIIKPTLKSLTEAYYKKDSRKICGLR 216

Query: 199 LDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSVCNTYVGNNPNPMDIVRI 258
            D+   LL+  N+ ANS VLVV+   GL+TG++AER+ G+G++ + Y+G  P+ + IV  
Sbjct: 217 FDSFGQLLTLGNIRANSQVLVVETCMGLVTGSIAERMNGQGTILSAYIGKGPS-LSIVNN 276

Query: 259 FNFDDE----------NCSRIMR-----SPLAELASSQV---EASKPNGEQEVGEQASQP 318
           F F+ +          N + ++      S L   ASS +   +  +   E+E  E     
Sbjct: 277 FGFNTDVLNTIYPFNLNITSVLNKGEDISKLPPTASSGIYDKQVKEKEKEKEKDENVKDE 336

Query: 319 LSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAP 378
               E      +Q +D ++E+IV                      L K+ G  SL+I   
Sbjct: 337 KESGEEAKTIINQRNDTSNENIV---------------------KLLKDGGVWSLVIVTK 396

Query: 379 QSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVL 438
            S   +++    P + +S  F IY Q+ QPL      L   +MA++ QISE W+RE+QVL
Sbjct: 397 YSPL-NILLSCWPYLNSSGSFVIYSQFPQPLMEVHQFLHKNQMAVNQQISEIWMREHQVL 447

Query: 439 PSRTHPFMQMNAFGGYILSGTKI 444
           P RTHP M M+   G+IL GTK+
Sbjct: 457 PKRTHPMMGMDGASGFILYGTKV 447

[Close]

BLAST of Lsi08G013280 vs. ExPASy Swiss-Prot
Match: Q2T9V5 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Bos taurus OX=9913 GN=TRMT6 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.8e-47
Identity = 142/454 (31.28%), Postives = 220/454 (48.46%), Query Frame = 0


Query: 27  INDGDRLVFARLSPASTLKIGNKS--------FSLKPLIGCPFGSLFQIENGGNGPHLAR 86
           I DGD +V  R      +++  +         F L  +IG  +G+ F++ NGG+      
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNIIGHSYGTTFEVTNGGS------ 79

Query: 87  VIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEI 146
           + P +++       EE   E  TK+   DNR I+D+ K+Q L+ +DI A++ +G  G+EI
Sbjct: 80  LQPKKKK-------EEPTSE--TKEAGTDNRNIIDDGKSQKLTQDDIKALKDKGIKGEEI 139

Query: 147 VEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLR 206
           V+ LI NS TF  KT F+Q+KY  +K+KKY   + + +P TR +   Y+ + P +I  +R
Sbjct: 140 VQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAMITVVKPSTRILSVMYYAREPGKINHMR 199

Query: 207 LDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSVCNTYVGNNPNPMDIVRI 266
            D L+ +L+  N+ A + ++V++   GL+ GA+ ER+GG GS+   Y G  P        
Sbjct: 200 YDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGP-VRAATAC 259

Query: 267 FNFDDENCSRIMRSPL------------AELASSQ----VEASKPNGEQEVGEQASQPLS 326
           F F     S +   PL            AE+ SS+        + NG  E  + + Q   
Sbjct: 260 FGFPKSFLSGLYEFPLNKVDSLLNGTFSAEMLSSEPKDIASVEESNGTLEEKQTSEQENE 319

Query: 327 MDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAP----------------PEVINL 386
              +  P ++   +     IVS      +  ++G K                   E   L
Sbjct: 320 DSIAEAPESNHPEEQERMEIVSQDPDYKEPKESGSKKDYIQEKQRRQEEQKKRHLEAAAL 379

Query: 387 WKENGFTSLIIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAIS 441
             E     LI+A+ +     L+  LL  VA S PF +Y QY +PL  C  KL+     I+
Sbjct: 380 LSERNADGLIVAS-RFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVIN 439

[Close]

BLAST of Lsi08G013280 vs. ExPASy Swiss-Prot
Match: Q8CE96 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Mus musculus OX=10090 GN=Trmt6 PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 6.3e-47
Identity = 148/453 (32.67%), Postives = 224/453 (49.45%), Query Frame = 0


Query: 27  INDGDRLVFARLSPASTLKIGNKS--------FSLKPLIGCPFGSLFQIENGGNGPHLAR 86
           I+DGD +V  R      +++  +         F L   IG  +GS F + +GG+      
Sbjct: 21  IHDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNAIGHSYGSAFDVSSGGS------ 80

Query: 87  VIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDEI 146
            +  R      ++ EE   E  TK+   DNR IVD+ K+Q L+ +DI A++ +G  G+EI
Sbjct: 81  -LQLR------KKLEEPASE--TKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEEI 140

Query: 147 VEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLR 206
           V+ LI NS TF  KT F+Q+KY  +K+KKY   V + +P TR +   Y+ + P +I  +R
Sbjct: 141 VQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIVTILKPSTRILSIMYYAREPGKINHMR 200

Query: 207 LDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSVCNTYVGNNPNPMDIVRI 266
            D L+ +L+  N+ A + ++V++   GL+ GA+ ER+GG GS+   Y G+ P        
Sbjct: 201 YDTLAQMLTLGNIRAGNKMIVMETCSGLVLGAMMERMGGFGSIIQLYPGDGP-VRAATAC 260

Query: 267 FNFDDENCSRIMRSPL------------AELASSQVEASKP----NG---EQEVGEQASQ 326
           F F     S +   PL            AE+ SS+ + S P    NG   E+E+ EQA +
Sbjct: 261 FGFPKSFLSGLYEFPLNKVNSLLNGTFSAEMLSSEPKDSTPVEESNGELEEKEIAEQADE 320

Query: 327 PLSMDESPLPSTDQ------------SSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLW 386
              +D +   S +Q                   S    + +  +  +   K   E   L 
Sbjct: 321 DNIVDAAENNSGEQRPMEIVPGDPENKEPKEKRSKRDYIQEKQRRQEEQRKRHLEAAALL 380

Query: 387 KENGFTSLIIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISL 441
            E     LI+A+ +     L+  LL  VA S PF +Y QY +PL  C  KL+     I+L
Sbjct: 381 GERNADGLIVAS-RFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVINL 440

[Close]

BLAST of Lsi08G013280 vs. ExPASy Swiss-Prot
Match: Q9UJA5 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Homo sapiens OX=9606 GN=TRMT6 PE=1 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.1e-46
Identity = 140/455 (30.77%), Postives = 219/455 (48.13%), Query Frame = 0


Query: 27  INDGDRLVFARLSPASTLKIGNKS--------FSLKPLIGCPFGSLFQIENGGN-GPHLA 86
           I DGD +V  R      +++  +         F L  +IG  +G+ F++ +GG+  P   
Sbjct: 20  IRDGDFVVLKREDVFKAVQVQRRKKVTFEKQWFYLDNVIGHSYGTAFEVTSGGSLQPKKK 79

Query: 87  RVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQGATGDE 146
           R  P+ E                TK+   DNR IVD+ K+Q L+ +DI A++ +G  G+E
Sbjct: 80  REEPTAE----------------TKEAGTDNRNIVDDGKSQKLTQDDIKALKDKGIKGEE 139

Query: 147 IVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFL 206
           IV+ LI NS TF  KT F+Q+KY  +K+KKY   + + +P TR +   Y+ + P +I  +
Sbjct: 140 IVQQLIENSTTFRDKTEFAQDKYIKKKKKKYEAIITVVKPSTRILSIMYYAREPGKINHM 199

Query: 207 RLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSVCNTYVGNNPNPMDIVR 266
           R D L+ +L+  N+ A + ++V++   GL+ GA+ ER+GG GS+   Y G  P       
Sbjct: 200 RYDTLAQMLTLGNIRAGNKMIVMETCAGLVLGAMMERMGGFGSIIQLYPGGGP-VRAATA 259

Query: 267 IFNFDDENCSRIMRSPL------------AELASSQVEAS----KPNGEQEVGEQASQPL 326
            F F     S +   PL            A++ SS+ + S    + NG  E  + + Q  
Sbjct: 260 CFGFPKSFLSGLYEFPLNKVDSLLHGTFSAKMLSSEPKDSALVEESNGTLEEKQASEQEN 319

Query: 327 SMDESPLPSTDQSSDLNSESIVSP----------------LTKVFKCAKAGDKAPPEVIN 386
               +  P ++   D  +   +S                 + +  +  +   K   E   
Sbjct: 320 EDSMAEAPESNHPEDQETMETISQDPEHKGPKERGSKKDYIQEKQRRQEEQRKRHLEAAA 379

Query: 387 LWKENGFTSLIIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAI 441
           L  E     LI+A+ +     L+  LL  VA S PF +Y QY +PL  C  KL+     I
Sbjct: 380 LLSERNADGLIVAS-RFHPTPLLLSLLDFVAPSRPFVVYCQYKEPLLECYTKLRERGGVI 439

[Close]

BLAST of Lsi08G013280 vs. ExPASy Swiss-Prot
Match: Q6BKK7 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=TRM6 PE=3 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 9.4e-35
Identity = 116/421 (27.55%), Postives = 209/421 (49.64%), Query Frame = 0


Query: 38  LSPASTLKIGN-KSFSLKPLIGCPFGSLFQIENGGNGPHLARVIPSREEGINCQETE-EM 97
           L P   + +G   +F++  ++G PFG  F+I          +V P +   +   ET  + 
Sbjct: 50  LKPGGIISLGKFGTFAVDGILGYPFGQTFEILEE------LKVKPIKSMTMQADETSVDN 109

Query: 98  KEEQVTKDELR--------DNRAIVD-NNKAQSLSSEDIDAMRSQGAT---GDEIVEALI 157
           + E++TKD+L         +N+ I++  +K Q L+SED+D ++  GAT   G  I+E +I
Sbjct: 110 ENEELTKDDLTKMLSNSSDNNQNIINIGSKIQKLTSEDVDKLKESGATSDIGQRIIEQMI 169

Query: 158 ANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPARIGFLRLDALS 217
           A    F+KKT+FSQ+KY  RKQ+K+  R  +    +  + + Y +K   R+  +  + L 
Sbjct: 170 AGHEGFDKKTLFSQQKYLKRKQQKFLRRFTVEYLGSSQLLQYYIEKDTQRVLDMSEETLG 229

Query: 218 LLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSVCNTYVGNNPNPMDIVRIFNFDD 277
           LLL++ANV      L++D  GG++  A+ ER+ G+G++ + +   +PN +  +R  ++ +
Sbjct: 230 LLLNYANVRPGGKYLLIDETGGIILYAMMERMNGQGTIVSAHDNEHPNHI-ALRYSDYPE 289

Query: 278 ENCSRIMRSPLAELASSQVEASKPNGEQEVGEQASQPLSMDESPLPSTDQ-SSDLNSESI 337
           E  +R+++S +  L   + E  K   E    E+  +      +     +Q + ++NS   
Sbjct: 290 EMQNRMVKS-INWLQFLEPENEKVEFETATEEEIEEMKHAKRAQYYRREQRAKNINS--- 349

Query: 338 VSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQSDAWSLVKDLLPLVANSAPFA 397
                               VI++  E  F    I+    +  +L+ +++P +  S P  
Sbjct: 350 --------------------VIDMVMEGNFDG-FISVSTLNMPTLLPEIIPTIGGSRPVV 409

Query: 398 IYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRTHPFMQMNAFGGYILSGTK 444
           IY Q+ + L    H +  +K  ++  I E  +R +Q +P R HP M M  FGGYIL GT+
Sbjct: 410 IYSQFKEALLETQHHMSTDKRVLAPSIMETRVRPHQTIPGRMHPVMCMRGFGGYILWGTR 438

[Close]

BLAST of Lsi08G013280 vs. ExPASy TrEMBL
Match: A0A1S3BB68 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis melo OX=3656 GN=LOC103488037 PE=3 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 4.9e-236
Identity = 418/446 (93.72%), Postives = 434/446 (97.31%), Query Frame = 0


Query: 2   SSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 61
           S++ILQS+S RNPRLTWEGCSVLLD+NDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLS 121
           GSLFQIENG NGP LARV+PSREEGINCQETEEMKEEQVTKDELRDNRA+VDNNKAQSLS
Sbjct: 63  GSLFQIENGENGPQLARVMPSREEGINCQETEEMKEEQVTKDELRDNRALVDNNKAQSLS 122

Query: 122 SEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 181
           SEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS
Sbjct: 123 SEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 182

Query: 182 ICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSV 241
           ICEAYFKKYPARIGFLRLDALSLLLSF NVTANSDVLVVDMVGGLLTGAVAER+GGKGSV
Sbjct: 183 ICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSV 242

Query: 242 CNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVGEQASQPL 301
           CNTYVGN PNPMDIVRIFNFDDE CSRIMRS LA+L SSQ+E +KPNGE +V EQASQP+
Sbjct: 243 CNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENKVSEQASQPV 302

Query: 302 SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQ 361
           SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQ
Sbjct: 303 SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQ 362

Query: 362 SDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLP 421
           SDAW LVKDLLPLVA+SAPFAIYHQYLQPLATCMHKLQLEKMAI+LQISEPWLREYQVLP
Sbjct: 363 SDAWRLVKDLLPLVASSAPFAIYHQYLQPLATCMHKLQLEKMAINLQISEPWLREYQVLP 422

Query: 422 SRTHPFMQMNAFGGYILSGTKICNHQ 448
           SRTHPFMQMNAFGGYILSGTKIC+HQ
Sbjct: 423 SRTHPFMQMNAFGGYILSGTKICDHQ 448

[Close]

BLAST of Lsi08G013280 vs. ExPASy TrEMBL
Match: A0A0A0LRN2 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis sativus OX=3659 GN=Csa_2G367740 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.5e-234
Identity = 422/452 (93.36%), Postives = 435/452 (96.24%), Query Frame = 0


Query: 1   MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MS +ILQSES RNPRLTWEGCSVLLD+NDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPHLARVIPSR-EEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENGGNGP LARV+PSR EEGI+CQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRL+KQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKG 240
           RSICEAYFKKYPARIGFLRLDALSLLLSF NVTANSDVLVVDMVGGLLTGAVAER+GGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQE----VGE 300
           SVCNTYVGN PNPMDIVRIFNFDDE CSRIMRS LA+L SSQ+E +KPNGE E    V E
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 QASQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360
           QASQP+SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360

Query: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLR 420
           IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQY+QPLATCMHKLQLEKMAISLQISEPWLR
Sbjct: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYVQPLATCMHKLQLEKMAISLQISEPWLR 420

Query: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICNHQ 448
           EYQVLPSRTHPFMQMNAFGGYILSGTKIC+ Q
Sbjct: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICDRQ 452

[Close]

BLAST of Lsi08G013280 vs. ExPASy TrEMBL
Match: A0A6J1CS60 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Momordica charantia OX=3673 GN=LOC111014079 PE=3 SV=1)

HSP 1 Score: 788.5 bits (2035), Expect = 1.5e-224
Identity = 404/452 (89.38%), Postives = 426/452 (94.25%), Query Frame = 0


Query: 1   MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSS  LQSE  RNPR+TWEGCSVLLDINDGDRLVFARLSPASTLKIGNKS+SLKPLIGCP
Sbjct: 1   MSSDNLQSELHRNPRVTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIEN   GPHL+RV+PSREEGINCQETE+MKEEQVTKDELRDNRAIVDNNKAQSL
Sbjct: 61  FGSLFQIENVAGGPHLSRVMPSREEGINCQETEKMKEEQVTKDELRDNRAIVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVL+RRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLVRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGS 240
           SICEAYFKKYPARIGFLR+DALSLLLSFANVT+NSDVLV+DMVGGLLTGAVAER+GGKGS
Sbjct: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTSNSDVLVLDMVGGLLTGAVAERLGGKGS 240

Query: 241 VCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVG----EQ 300
           VCNTY+GNNPN MDIVRIFNFDDE C+RIMRSPL +LASSQV++SK NGE E      EQ
Sbjct: 241 VCNTYLGNNPNHMDIVRIFNFDDEICARIMRSPLIDLASSQVDSSKSNGEHEASINAEEQ 300

Query: 301 ASQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLI 360
           AS+P+SMDES +PSTDQ S+LNSESIVSPLTK FKCAKAGDKAP EV+N WKENGFTSLI
Sbjct: 301 ASEPVSMDESAVPSTDQPSELNSESIVSPLTKAFKCAKAGDKAPSEVVNSWKENGFTSLI 360

Query: 361 IAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQ DAWSLVKDLLPLV+NSAPFAIYHQYLQPLATCMHKLQLE+MAISLQISEPWLRE
Sbjct: 361 IAAPQLDAWSLVKDLLPLVSNSAPFAIYHQYLQPLATCMHKLQLERMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC-NHQ 448
           YQVLPSRTHPFMQMNAFGGYILSGTKIC  HQ
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICGGHQ 452

[Close]

BLAST of Lsi08G013280 vs. ExPASy TrEMBL
Match: A0A5A7VI74 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005060 PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.3e-216
Identity = 391/422 (92.65%), Postives = 406/422 (96.21%), Query Frame = 0


Query: 2   SSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 61
           S++ILQS+S RNPRLTWEGCSVLLD+NDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLS 121
           GSLFQIENG NGP LARV+PSREEGINCQETEEMKEEQVTKDELRDNRA+VDNNKAQSLS
Sbjct: 63  GSLFQIENGENGPQLARVMPSREEGINCQETEEMKEEQVTKDELRDNRALVDNNKAQSLS 122

Query: 122 SEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 181
           SEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS
Sbjct: 123 SEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 182

Query: 182 ICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSV 241
           ICEAYFKKYPARIGFLRLDALSLLLSF NVTANSDVLVVDMVGGLLTGAVAER+GGKGSV
Sbjct: 183 ICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSV 242

Query: 242 CNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVG----EQA 301
           CNTYVGN PNPMDIVRIFNFDDE CSRIMRS LA+L SSQ+E +KPNGE +V     EQA
Sbjct: 243 CNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENKVSVNVQEQA 302

Query: 302 SQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLII 361
           SQP+SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLII
Sbjct: 303 SQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLII 362

Query: 362 AAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREY 420
           AAPQSDAW LVKDLLPLVA+SAPFAIYHQYLQPLATCMHKLQLEKMAI+LQISEPWLREY
Sbjct: 363 AAPQSDAWRLVKDLLPLVASSAPFAIYHQYLQPLATCMHKLQLEKMAINLQISEPWLREY 422

[Close]

BLAST of Lsi08G013280 vs. ExPASy TrEMBL
Match: A0A6J1GI99 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbita moschata OX=3662 GN=LOC111454455 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.6e-216
Identity = 393/449 (87.53%), Postives = 416/449 (92.65%), Query Frame = 0


Query: 1   MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSSS LQSESPRNPR+ WEGCSVLLDINDGDRLVFARLSPASTLKIGNKS+SLKPLIG P
Sbjct: 1   MSSSNLQSESPRNPRVAWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGRP 60

Query: 61  FGSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIE+G NG HLA V PSREE     ETEEMKEEQV KDELRDNRA+VDNNKAQSL
Sbjct: 61  FGSLFQIESGDNGQHLACVTPSREE-----ETEEMKEEQVVKDELRDNRALVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRL+KQKKYAPRVLLRRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLKKQKKYAPRVLLRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGS 240
           SICEAYFKK+PARIGFLR+DALSLLLSFANVT+NSDVLV DMVGGL+TGAVAER+GGKGS
Sbjct: 181 SICEAYFKKFPARIGFLRVDALSLLLSFANVTSNSDVLVFDMVGGLITGAVAERLGGKGS 240

Query: 241 VCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEV----GEQ 300
           VCNTY+G+NPNPM+IVR+FNFDDE C+RIMRSPL +L SSQV     NGE EV     ++
Sbjct: 241 VCNTYLGSNPNPMEIVRMFNFDDEICARIMRSPLTDLTSSQVS----NGEHEVTIKAQDE 300

Query: 301 ASQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLI 360
           AS+P++MDE PLPSTDQSSDLNSESIVSPLTKV KCAKAGDKAPPEV+  WKENGFTSLI
Sbjct: 301 ASEPVNMDEGPLPSTDQSSDLNSESIVSPLTKVLKCAKAGDKAPPEVVKSWKENGFTSLI 360

Query: 361 IAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQSDAW LVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE
Sbjct: 361 IAAPQSDAWGLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICN 446
           YQVLPSRTHPFMQMNAFGGYILSGTKIC+
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICD 440

[Close]

BLAST of Lsi08G013280 vs. NCBI nr
Match: XP_038884150.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Benincasa hispida])

HSP 1 Score: 851.3 bits (2198), Expect = 3.8e-243
Identity = 436/451 (96.67%), Postives = 443/451 (98.23%), Query Frame = 0


Query: 1   MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSSSILQSESPRNPRLTWEGCSVLLD+NDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSSSILQSESPRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIENGGNGPHLARV+PSREEG NCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL
Sbjct: 61  FGSLFQIENGGNGPHLARVMPSREEGNNCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SS+DIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR
Sbjct: 121 SSDDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGS 240
           SICEAYFKKYPARIGFLRLDALSLLLSFANVTA+SDVLVVDMVGGLLTGAVAER+GGKGS
Sbjct: 181 SICEAYFKKYPARIGFLRLDALSLLLSFANVTAHSDVLVVDMVGGLLTGAVAERLGGKGS 240

Query: 241 VCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVG----EQ 300
           VCNTYVGNNPNPMDIVRIFNFDDE CSRIMRSPLAELASSQVE SKPNGEQEVG    +Q
Sbjct: 241 VCNTYVGNNPNPMDIVRIFNFDDEICSRIMRSPLAELASSQVEDSKPNGEQEVGINLHDQ 300

Query: 301 ASQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLI 360
           ASQP+SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAP EVINLWKENGFTSLI
Sbjct: 301 ASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPQEVINLWKENGFTSLI 360

Query: 361 IAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE
Sbjct: 361 IAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICNHQ 448
           YQVLPSRTHPFMQMNAFGGYILSGTKICNHQ
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICNHQ 451

[Close]

BLAST of Lsi08G013280 vs. NCBI nr
Match: XP_008444799.1 (PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Cucumis melo])

HSP 1 Score: 826.6 bits (2134), Expect = 1.0e-235
Identity = 418/446 (93.72%), Postives = 434/446 (97.31%), Query Frame = 0


Query: 2   SSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 61
           S++ILQS+S RNPRLTWEGCSVLLD+NDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF
Sbjct: 3   STTILQSDSRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPF 62

Query: 62  GSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLS 121
           GSLFQIENG NGP LARV+PSREEGINCQETEEMKEEQVTKDELRDNRA+VDNNKAQSLS
Sbjct: 63  GSLFQIENGENGPQLARVMPSREEGINCQETEEMKEEQVTKDELRDNRALVDNNKAQSLS 122

Query: 122 SEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 181
           SEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS
Sbjct: 123 SEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRS 182

Query: 182 ICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSV 241
           ICEAYFKKYPARIGFLRLDALSLLLSF NVTANSDVLVVDMVGGLLTGAVAER+GGKGSV
Sbjct: 183 ICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKGSV 242

Query: 242 CNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVGEQASQPL 301
           CNTYVGN PNPMDIVRIFNFDDE CSRIMRS LA+L SSQ+E +KPNGE +V EQASQP+
Sbjct: 243 CNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENKVSEQASQPV 302

Query: 302 SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQ 361
           SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQ
Sbjct: 303 SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQ 362

Query: 362 SDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLP 421
           SDAW LVKDLLPLVA+SAPFAIYHQYLQPLATCMHKLQLEKMAI+LQISEPWLREYQVLP
Sbjct: 363 SDAWRLVKDLLPLVASSAPFAIYHQYLQPLATCMHKLQLEKMAINLQISEPWLREYQVLP 422

Query: 422 SRTHPFMQMNAFGGYILSGTKICNHQ 448
           SRTHPFMQMNAFGGYILSGTKIC+HQ
Sbjct: 423 SRTHPFMQMNAFGGYILSGTKICDHQ 448

[Close]

BLAST of Lsi08G013280 vs. NCBI nr
Match: XP_004152653.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X2 [Cucumis sativus])

HSP 1 Score: 824.3 bits (2128), Expect = 5.0e-235
Identity = 421/448 (93.97%), Postives = 434/448 (96.88%), Query Frame = 0


Query: 1   MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MS +ILQSES RNPRLTWEGCSVLLD+NDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPHLARVIPSR-EEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENGGNGP LARV+PSR EEGI+CQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRL+KQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKG 240
           RSICEAYFKKYPARIGFLRLDALSLLLSF NVTANSDVLVVDMVGGLLTGAVAER+GGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVGEQASQ 300
           SVCNTYVGN PNPMDIVRIFNFDDE CSRIMRS LA+L SSQ+E +KPNGE E  EQASQ
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSEQASQ 300

Query: 301 PLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAA 360
           P+SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAA
Sbjct: 301 PVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAA 360

Query: 361 PQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQV 420
           PQSDAWSLVKDLLPLVANSAPFAIYHQY+QPLATCMHKLQLEKMAISLQISEPWLREYQV
Sbjct: 361 PQSDAWSLVKDLLPLVANSAPFAIYHQYVQPLATCMHKLQLEKMAISLQISEPWLREYQV 420

Query: 421 LPSRTHPFMQMNAFGGYILSGTKICNHQ 448
           LPSRTHPFMQMNAFGGYILSGTKIC+ Q
Sbjct: 421 LPSRTHPFMQMNAFGGYILSGTKICDRQ 448

[Close]

BLAST of Lsi08G013280 vs. NCBI nr
Match: XP_011649656.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X1 [Cucumis sativus] >KGN62671.1 hypothetical protein Csa_022108 [Cucumis sativus])

HSP 1 Score: 820.5 bits (2118), Expect = 7.2e-234
Identity = 422/452 (93.36%), Postives = 435/452 (96.24%), Query Frame = 0


Query: 1   MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MS +ILQSES RNPRLTWEGCSVLLD+NDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP
Sbjct: 1   MSLTILQSESRRNPRLTWEGCSVLLDVNDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPHLARVIPSR-EEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120
           FGSLFQIENGGNGP LARV+PSR EEGI+CQETEEMKEEQVTKDELRDNRAIVDNNKAQS
Sbjct: 61  FGSLFQIENGGNGPELARVMPSREEEGISCQETEEMKEEQVTKDELRDNRAIVDNNKAQS 120

Query: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFT 180
           LSSEDIDAMRSQGATGDEIVEALIANSATF+KKTVFSQEKYRL+KQKKYAPRVLLRRPFT
Sbjct: 121 LSSEDIDAMRSQGATGDEIVEALIANSATFDKKTVFSQEKYRLKKQKKYAPRVLLRRPFT 180

Query: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKG 240
           RSICEAYFKKYPARIGFLRLDALSLLLSF NVTANSDVLVVDMVGGLLTGAVAER+GGKG
Sbjct: 181 RSICEAYFKKYPARIGFLRLDALSLLLSFGNVTANSDVLVVDMVGGLLTGAVAERLGGKG 240

Query: 241 SVCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQE----VGE 300
           SVCNTYVGN PNPMDIVRIFNFDDE CSRIMRS LA+L SSQ+E +KPNGE E    V E
Sbjct: 241 SVCNTYVGNTPNPMDIVRIFNFDDEICSRIMRSSLADLTSSQIEPTKPNGENEDSINVQE 300

Query: 301 QASQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360
           QASQP+SMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL
Sbjct: 301 QASQPVSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSL 360

Query: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLR 420
           IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQY+QPLATCMHKLQLEKMAISLQISEPWLR
Sbjct: 361 IIAAPQSDAWSLVKDLLPLVANSAPFAIYHQYVQPLATCMHKLQLEKMAISLQISEPWLR 420

Query: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICNHQ 448
           EYQVLPSRTHPFMQMNAFGGYILSGTKIC+ Q
Sbjct: 421 EYQVLPSRTHPFMQMNAFGGYILSGTKICDRQ 452

[Close]

BLAST of Lsi08G013280 vs. NCBI nr
Match: XP_022144389.1 (tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Momordica charantia])

HSP 1 Score: 788.5 bits (2035), Expect = 3.0e-224
Identity = 404/452 (89.38%), Postives = 426/452 (94.25%), Query Frame = 0


Query: 1   MSSSILQSESPRNPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCP 60
           MSS  LQSE  RNPR+TWEGCSVLLDINDGDRLVFARLSPASTLKIGNKS+SLKPLIGCP
Sbjct: 1   MSSDNLQSELHRNPRVTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSYSLKPLIGCP 60

Query: 61  FGSLFQIENGGNGPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSL 120
           FGSLFQIEN   GPHL+RV+PSREEGINCQETE+MKEEQVTKDELRDNRAIVDNNKAQSL
Sbjct: 61  FGSLFQIENVAGGPHLSRVMPSREEGINCQETEKMKEEQVTKDELRDNRAIVDNNKAQSL 120

Query: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTR 180
           SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVL+RRPFTR
Sbjct: 121 SSEDIDAMRSQGATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLVRRPFTR 180

Query: 181 SICEAYFKKYPARIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGS 240
           SICEAYFKKYPARIGFLR+DALSLLLSFANVT+NSDVLV+DMVGGLLTGAVAER+GGKGS
Sbjct: 181 SICEAYFKKYPARIGFLRVDALSLLLSFANVTSNSDVLVLDMVGGLLTGAVAERLGGKGS 240

Query: 241 VCNTYVGNNPNPMDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQEVG----EQ 300
           VCNTY+GNNPN MDIVRIFNFDDE C+RIMRSPL +LASSQV++SK NGE E      EQ
Sbjct: 241 VCNTYLGNNPNHMDIVRIFNFDDEICARIMRSPLIDLASSQVDSSKSNGEHEASINAEEQ 300

Query: 301 ASQPLSMDESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLI 360
           AS+P+SMDES +PSTDQ S+LNSESIVSPLTK FKCAKAGDKAP EV+N WKENGFTSLI
Sbjct: 301 ASEPVSMDESAVPSTDQPSELNSESIVSPLTKAFKCAKAGDKAPSEVVNSWKENGFTSLI 360

Query: 361 IAAPQSDAWSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLRE 420
           IAAPQ DAWSLVKDLLPLV+NSAPFAIYHQYLQPLATCMHKLQLE+MAISLQISEPWLRE
Sbjct: 361 IAAPQLDAWSLVKDLLPLVSNSAPFAIYHQYLQPLATCMHKLQLERMAISLQISEPWLRE 420

Query: 421 YQVLPSRTHPFMQMNAFGGYILSGTKIC-NHQ 448
           YQVLPSRTHPFMQMNAFGGYILSGTKIC  HQ
Sbjct: 421 YQVLPSRTHPFMQMNAFGGYILSGTKICGGHQ 452

[Close]

BLAST of Lsi08G013280 vs. TAIR 10
Match: AT2G45730.1 (eukaryotic initiation factor 3 gamma subunit family protein )

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-147
Identity = 269/439 (61.28%), Postives = 339/439 (77.22%), Query Frame = 0


Query: 13  NPRLTWEGCSVLLDINDGDRLVFARLSPASTLKIGNKSFSLKPLIGCPFGSLFQIENGGN 72
           NPR   EGCSVLLDINDGDRLVFARLS  + LKIGNK++SLKPLIG PFGSLFQ+E G +
Sbjct: 20  NPRFACEGCSVLLDINDGDRLVFARLSSGAILKIGNKNYSLKPLIGAPFGSLFQVETGED 79

Query: 73  GPHLARVIPSREEGINCQETEEMKEEQVTKDELRDNRAIVDNNKAQSLSSEDIDAMRSQG 132
           G  L+R++P ++E                 D+ RDNR IVDNN+AQ+L+ E+I+AMR +G
Sbjct: 80  GSFLSRILPIKQE----------SNSNNVMDDSRDNREIVDNNEAQNLTGEEIEAMRREG 139

Query: 133 ATGDEIVEALIANSATFEKKTVFSQEKYRLRKQKKYAPRVLLRRPFTRSICEAYFKKYPA 192
           A GDEI+EALIANS TF+KK   SQEKY+L+KQKKYAP++LLRRPF RSICEAYFKKYPA
Sbjct: 140 AKGDEIIEALIANSKTFDKKFQLSQEKYKLKKQKKYAPKLLLRRPFARSICEAYFKKYPA 199

Query: 193 RIGFLRLDALSLLLSFANVTANSDVLVVDMVGGLLTGAVAERIGGKGSVCNTYVGNNPNP 252
           RIGFLR+DALSLLL+ ANVTA SDVLVVDMVGGL+TGAVAER+GG G VCNTY G++P P
Sbjct: 200 RIGFLRVDALSLLLTMANVTAYSDVLVVDMVGGLVTGAVAERLGGTGYVCNTYKGSSPYP 259

Query: 253 MDIVRIFNFDDENCSRIMRSPLAELASSQVEASKPNGEQE--------VGEQASQPLSMD 312
           +++VR+FNF D+   RI++S + EL+S++  +++ N +QE        + E A+  + ++
Sbjct: 260 VEMVRMFNFSDKVLKRIVQSSINELSSAKTASTEGNNQQEGVCKAENNINETATSDVMVE 319

Query: 313 ESPLPSTDQSSDLNSESIVSPLTKVFKCAKAGDKAPPEVINLWKENGFTSLIIAAPQSDA 372
           E  +     +++     IV P +K+ K AKAG KA  E I +W+ENGF+SLI+AA   D 
Sbjct: 320 EISV-----TTEAGVVDIVVPESKIIKAAKAGAKASKETIEMWQENGFSSLIMAAQDQDP 379

Query: 373 WSLVKDLLPLVANSAPFAIYHQYLQPLATCMHKLQLEKMAISLQISEPWLREYQVLPSRT 432
           W + KD+LPL++ SAPFAIYHQYLQPLATCMHKLQ  KMAI+LQI+EPWLREYQVLPSR+
Sbjct: 380 WIVAKDVLPLLSYSAPFAIYHQYLQPLATCMHKLQQGKMAINLQITEPWLREYQVLPSRS 439

Query: 433 HPFMQMNAFGGYILSGTKI 444
           HP MQM++FGGY+LSG +I
Sbjct: 440 HPHMQMSSFGGYVLSGIRI 443

The following BLAST results are available for this feature:

BLAST of Lsi08G013280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54UB1	7.6e-53	32.96	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 OS=Dictyost...	[more]
Q2T9V5	4.8e-47	31.28	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Bos taur...	[more]
Q8CE96	6.3e-47	32.67	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Mus musc...	[more]
Q9UJA5	4.1e-46	30.77	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Homo sap...	[more]
Q6BKK7	9.4e-35	27.55	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Debaryom...	[more]

BLAST of Lsi08G013280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BB68	4.9e-236	93.72	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis ...	[more]
A0A0A0LRN2	3.5e-234	93.36	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis ...	[more]
A0A6J1CS60	1.5e-224	89.38	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Momordic...	[more]
A0A5A7VI74	3.3e-216	92.65	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucumis ...	[more]
A0A6J1GI99	9.6e-216	87.53	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6 OS=Cucurbit...	[more]

BLAST of Lsi08G013280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884150.1	3.8e-243	96.67	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Benincasa ...	[more]
XP_008444799.1	1.0e-235	93.72	PREDICTED: tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 ...	[more]
XP_004152653.1	5.0e-235	93.97	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X2 ...	[more]
XP_011649656.1	7.2e-234	93.36	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 isoform X1 ...	[more]
XP_022144389.1	3.0e-224	89.38	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit trm6 [Momordica ...	[more]

BLAST of Lsi08G013280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45730.1	1.1e-147	61.28	eukaryotic initiation factor 3 gamma subunit family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017423	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6	PIRSF	PIRSF038170	TRM6	coord: 8..447 e-value: 1.3E-129 score: 430.7
IPR017423	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6	PFAM	PF04189	Gcd10p	coord: 22..282 e-value: 1.0E-61 score: 208.8
IPR017423	tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6	PANTHER	PTHR12945	TRANSLATION INITIATION FACTOR EIF3-RELATED	coord: 12..446
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 280..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G013280.1	Lsi08G013280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007049	cell cycle
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005667	transcription regulator complex
cellular_component	GO:0031515	tRNA (m1A) methyltransferase complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity