Lsi08G013130 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G013130 vs. ExPASy Swiss-Prot
Match: Q9SA91 (Protein PSK SIMULATOR 2 OS=Arabidopsis thaliana OX=3702 GN=PSI2 PE=2 SV=2)

HSP 1 Score: 322.8 bits (826), Expect = 7.5e-87
Identity = 233/607 (38.39%), Postives = 333/607 (54.86%), Query Frame = 0

Query: 5   MGGVCSNGIVKDDFVSEKITQASEDR------KGNSCLNFEASDP-------NEMPQKSR 64
           MGGVCS  + KDD   +K+    +D+      K  S    + SD            + S+
Sbjct: 1   MGGVCS-CVFKDDDKKKKLRSNDDDKSRGFSGKLKSMRRSKTSDSYYSDNYGGSRRKSSK 60

Query: 65  SGVILL-------PSPPSKTGNNKVAPMNAQSGARG-----RAVDLWKTIGISVSNLHMN 124
              ++        P PP +  + K    N+  G  G     +AV++  T+G S++ ++ +
Sbjct: 61  PDEVVFNFSGELGPMPPLRNDSTKFMQRNSFMGRAGVMGLEKAVEVLDTLGSSMTRMNPS 120

Query: 125 SGLFTGMTSN-GREISILAFEVANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLIST 184
           +   +G+TS+ G +++ILAFEVANTI+K A L QSLSEEN++ +KK++L SE +K+L+ST
Sbjct: 121 NAYLSGVTSSRGGKVTILAFEVANTIAKGAALLQSLSEENLKFMKKDMLHSEEVKKLVST 180

Query: 185 SLEELLSIAAADKRQEFDVVSREVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQAL-EA 244
              EL  +AA+DKR+E D+ S EVIRFG  CKD QWHNLD+YF +LD  +S  K    +A
Sbjct: 181 DTTELQILAASDKREELDLFSGEVIRFGNMCKDLQWHNLDRYFMKLDTENSQHKLLKDDA 240

Query: 245 RATLRELTVLAQYTSELYHELQALERFEQDYRRKVDEVEFLNQAGIGWLAAGIQLL---- 304
            A ++EL  LA+ TSELYHELQAL+RFEQDYRRK+ EVE LN    G    GI +L    
Sbjct: 241 EARMQELVTLARITSELYHELQALDRFEQDYRRKLAEVESLNLPRRG---EGIVILQNEL 300

Query: 305 ---------ISNSLLYKPTIAD-------------KTLLTKDRSNG----------QKLG 364
                    +    L+   +A+             +T++    +NG          ++LG
Sbjct: 301 KQQKKLVKSLQKKSLWSQNLAEIIEKLVDVVSYIRQTIVEVFGNNGLRDNEGEQGRERLG 360

Query: 365 AVGLALHYANIISQINLIACRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTY 424
             GL+LHYAN+I QI+ IA RP+S+PSN+RD LY ALP +VK ALR RL+ +D  EE + 
Sbjct: 361 EAGLSLHYANLIQQIDNIASRPSSLPSNVRDTLYNALPATVKTALRPRLQTLDQEEELSV 420

Query: 425 LDVKAEMDKILEWFVPIAANTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSN--NPI 484
            ++KAEM+K L+W VP A NT+     +AHQ  G +GEWA    E  KG+    N  NP 
Sbjct: 421 PEIKAEMEKSLQWLVPFAENTT-----KAHQGFGWVGEWANSRIEFGKGKGKGENNGNPT 480

Query: 485 RLQTLYHADKVKTEQQILELVILLHHLIHLAKRQQRRSTSLRCRSPTPKDMANN--ARRI 541
           RLQTL+HADK   +  +LELV+ LH L+   K  ++R+  ++ +        NN      
Sbjct: 481 RLQTLHHADKPIVDSYVLELVVWLHRLM---KSSKKRAHGVKLQETNHVSPPNNRTISNT 540

BLAST of Lsi08G013130 vs. ExPASy Swiss-Prot
Match: Q9XID5 (Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2)

HSP 1 Score: 293.1 bits (749), Expect = 6.4e-78
Identity = 200/522 (38.31%), Postives = 292/522 (55.94%), Query Frame = 0

Query: 14  VKDDFVSEKITQASEDRKGNS-CLNFEASDPNEMPQKSRSG------VILLPSPPSKTGN 73
           +KDD     +T+  +D K  S   +F        PQ    G      V+   S  +K+  
Sbjct: 43  LKDD--PSPVTENVDDNKHTSESFSFPIVSSGSHPQNIEDGIPRLSRVLSQKSRSTKSRQ 102

Query: 74  NKVAPMNAQSGARGR--------AVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAF 133
             VA ++  S   GR        AVD+  T+G S++NL+++ G  +  T  G +ISIL+F
Sbjct: 103 AAVAKVSEVSSLLGRAGTMGLGKAVDVLDTLGSSMTNLNLSGGFSSATTVKGNKISILSF 162

Query: 134 EVANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVV 193
           EVANTI K A L  SLS+++I  LK+ VL SEG++ LIS  ++ELL IAAADKR+E  + 
Sbjct: 163 EVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDELLRIAAADKREELRIF 222

Query: 194 SREVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQ-ALEARATLRELTVLAQYTSELYHE 253
           S EV+RFG +CKD Q+HNLD++F RL    + QK    EA   + ++     +T++LYHE
Sbjct: 223 SGEVVRFGNRCKDPQYHNLDRFFDRLGSEFTPQKHLKQEAETIMHQMMSFVHFTADLYHE 282

Query: 254 LQALERFEQDYRRKVDEVE--FLNQAGIGWLAAGIQLLISNSLLYKPTIADKTLLTK--- 313
           L AL+RFEQDY+RK+ E E     Q G+G   A ++  + +   +   +  K+L ++   
Sbjct: 283 LHALDRFEQDYQRKIQEEENPSTAQRGVGDTLAILRTELKSQKKHVRNLKKKSLWSRILE 342

Query: 314 --------------------------DRS------NGQKLGAVGLALHYANIISQINLIA 373
                                     D+       N +KLG+ GLALHYANII+QI+ + 
Sbjct: 343 EVMEKLVDVVHFLHLEIHEAFGGADPDKPANDPPINHKKLGSAGLALHYANIITQIDTLV 402

Query: 374 CRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAA 433
            R +++P++ RDALY+ LP S+K ALRSR+++    EE T   +KAEM+K L+W VP+A 
Sbjct: 403 SRSSTMPASTRDALYQGLPPSIKSALRSRIQSFQVKEELTVPQIKAEMEKTLQWLVPVAT 462

Query: 434 NTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELV 482
           NT+     +AH   G +GEWA+   E ++  A Q+   +R+ TL+HADK KTE  IL+LV
Sbjct: 463 NTT-----KAHHGFGWVGEWASSGSEANQRPAGQT--ILRIDTLHHADKEKTEAYILDLV 522

BLAST of Lsi08G013130 vs. ExPASy Swiss-Prot
Match: P0DO24 (Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.6e-65
Identity = 159/412 (38.59%), Postives = 231/412 (56.07%), Query Frame = 0

Query: 80  GRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEVANTISKVAKLSQSLSEENI 139
           GRA D+  T+G S+++L  + G  +G+ + G E+ ILAFEVANTI K + L +SLS+ NI
Sbjct: 117 GRAKDVLDTLGSSMTDL-SSGGFTSGVATKGNELGILAFEVANTIVKSSNLIESLSKRNI 176

Query: 140 QLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSREVIRFGKQCKDSQWHNLDQ 199
           + LK  +L SEG++ L+S   +ELL + AADKRQE  V S EV+RFG + KD QWHNL +
Sbjct: 177 EHLKGTILYSEGVQNLVSNDFDELLRLVAADKRQELQVFSGEVVRFGNRSKDFQWHNLQR 236

Query: 200 YFSRLDFNDSSQKQALE-ARATLRELTVLAQYTSELYHELQALERFEQDYRRKVDEVEFL 259
           YF R+    + Q+Q  E A   + +L VL QYT+ELY ELQ L R E+DY +K  E E  
Sbjct: 237 YFDRISKELTPQRQLKEDAVLVVDQLMVLVQYTAELYQELQVLYRLEKDYEQKRREEENS 296

Query: 260 NQAGIGWLAAGIQLLISNSLLYKPTIADKTLLTKD------------------------- 319
             +  G   A ++  +        ++  K+L ++                          
Sbjct: 297 ANSSKGDGLAILKTELKAQRKVVKSLKKKSLWSRGFEEVMEKLVDIVHFLLLEIHNIFGG 356

Query: 320 ----------RSNGQKLGAVGLALHYANIISQINLIACRPTSIPSNMRDALYRALPTSVK 379
                         ++LG  GLALHYANII QI+ +  R +SI SN RD+LY++LP  +K
Sbjct: 357 ADDQPSKKGAAEYDKRLGPAGLALHYANIIVQIDTLVARASSITSNARDSLYQSLPPGIK 416

Query: 380 IALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTSNMIRDRAHQACGRIGEWATQ 439
           +ALRS++++ +  +E +   +K EM++ L W VP+A NT+     +AH   G +GEWA  
Sbjct: 417 LALRSKIKSFNVDKELSVTQIKDEMERTLHWLVPVAGNTT-----KAHHGFGWVGEWANT 476

Query: 440 SKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILLHHLIHLAKRQQR 456
             + +        + +R++TLYHA K KTE  IL  +I L HL+  AK   R
Sbjct: 477 GTDFTS--KPSGGDILRIETLYHASKEKTEIYILGQIIWLQHLVTKAKSDAR 520

BLAST of Lsi08G013130 vs. ExPASy TrEMBL
Match: A0A5A7VI62 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004950 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.4e-237
Identity = 455/579 (78.58%), Postives = 485/579 (83.77%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGIVKDDFVSEK+TQASEDRKGNS LNFEASDPNEMP+KS SGVILLPSP
Sbjct: 1   MGNTMGGVCSNGIVKDDFVSEKVTQASEDRKGNSHLNFEASDPNEMPEKSGSGVILLPSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSK G+NKVAPMNAQ+GARGRAVDLWKTIGISVSNLH+N+G FT MT +GREISILAFEV
Sbjct: 61  PSKAGSNKVAPMNAQAGARGRAVDLWKTIGISVSNLHINNGFFTAMTPSGREISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKVA LS+SLSEEN+QLLKKE+LQSEGIKQLIS SLEELLSIAAADKRQEF V+ R
Sbjct: 121 ANTISKVANLSKSLSEENMQLLKKELLQSEGIKQLISPSLEELLSIAAADKRQEFGVILR 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           EVIRFG QCKDS+WHNLDQYFSRLD NDSSQKQA EARA ++ELTVLAQ TSELYHELQA
Sbjct: 181 EVIRFGNQCKDSRWHNLDQYFSRLDSNDSSQKQAREARAAVQELTVLAQNTSELYHELQA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W---LAAGIQL 300
           LER EQDYRR+V+EVE LNQAGIG                         W   L   ++ 
Sbjct: 241 LERLEQDYRRRVEEVELLNQAGIGETLSIFQGELNVQRRLVRSFQSKCLWSRNLDEIVEK 300

Query: 301 LISNSLLYKPTI--------ADKTLLTKDRSNGQKLGAVGLALHYANIISQINLIACRPT 360
           L+        TI         DKTLL KDRSNGQKLGAVGLALHYANIISQINLIA RPT
Sbjct: 301 LVIVVTWINQTIVKEFGVDNTDKTLLIKDRSNGQKLGAVGLALHYANIISQINLIALRPT 360

Query: 361 SIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTSN 420
           SIPSNMRDALYRALPTS+KIALRSRLRAVDA EE TY DVKAEMDKILEW VPIAANTS 
Sbjct: 361 SIPSNMRDALYRALPTSIKIALRSRLRAVDAREEPTYYDVKAEMDKILEWLVPIAANTS- 420

Query: 421 MIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILLH 480
               +AHQ+CGRIGEWATQSKE SKGRATQSNNP RLQTLYHADKVKTE+QILELVILLH
Sbjct: 421 ----KAHQSCGRIGEWATQSKELSKGRATQSNNPNRLQTLYHADKVKTEKQILELVILLH 480

Query: 481 HLIHLAKRQQRRSTSLRCRSPTPKDMANNARRIQFKSQIIKANKDGVPAENRPLTGQSPT 540
           HLIH AK QQRRS+SLRCRSPTPKDMAN++RRIQFKSQII+  KDG P +N P   QSP 
Sbjct: 481 HLIHQAKHQQRRSSSLRCRSPTPKDMANSSRRIQFKSQIIRTTKDGFPTDNIPPASQSPI 540

Query: 541 RKRDLSNNKGMESYKNENKGIWTLSKAVSVSTLTSLGRV 544
           RKR L N KGMESYKNENKGIWTLSKAVSVSTL ++GRV
Sbjct: 541 RKRVLGNKKGMESYKNENKGIWTLSKAVSVSTLRTIGRV 574

BLAST of Lsi08G013130 vs. ExPASy TrEMBL
Match: A0A0A0LLM8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G368840 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.8e-233
Identity = 448/575 (77.91%), Postives = 476/575 (82.78%), Query Frame = 0

Query: 5   MGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSPPSKT 64
           MGGVCSNGIVKDDFVSEKI QASEDRKGNS LN EA DPNEMP+KSRS VILLPSPPSKT
Sbjct: 1   MGGVCSNGIVKDDFVSEKIIQASEDRKGNSYLNSEARDPNEMPEKSRSDVILLPSPPSKT 60

Query: 65  GNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEVANTI 124
           G+NKVAPMNAQ+GARGRAVDLWKTIGISVSN H+NSG+ TGM  +GREISILAFEVANTI
Sbjct: 61  GSNKVAPMNAQAGARGRAVDLWKTIGISVSNFHINSGVSTGMAPSGREISILAFEVANTI 120

Query: 125 SKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSREVIR 184
           SKVA LS+SLSEENIQLLK E+LQSE IKQLIS SLEELLSIAAADKRQEF V+ RE+IR
Sbjct: 121 SKVANLSKSLSEENIQLLKNELLQSEAIKQLISASLEELLSIAAADKRQEFGVILREIIR 180

Query: 185 FGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQALERF 244
           FG +CKDSQWHNLDQYFSRLD NDSSQKQA EARA L+ELTVLAQ TSELYHELQALER 
Sbjct: 181 FGNRCKDSQWHNLDQYFSRLDSNDSSQKQAREARAALQELTVLAQNTSELYHELQALERL 240

Query: 245 EQDYRRKVDEVEFLNQAGIG-------------------------W---LAAGIQLLISN 304
           EQDYRR+V+EVEFLNQAGIG                         W   L   ++ L+  
Sbjct: 241 EQDYRRRVEEVEFLNQAGIGETLSIFQGELNVQRKLVRSFQSKCLWSRNLDEIVEKLVIV 300

Query: 305 SLLYKPTI--------ADKTLLTKDRSNGQKLGAVGLALHYANIISQINLIACRPTSIPS 364
                 TI         DKTLL KDRSNGQKLGAVGLALHYANIISQINLIACRPTSIPS
Sbjct: 301 VTWINQTIIKEFGVDNTDKTLLIKDRSNGQKLGAVGLALHYANIISQINLIACRPTSIPS 360

Query: 365 NMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTSNMIRD 424
           NMRDALYRALPTS+KIALRSRLRAVDA EE TY DVK EMDKILEW VPIAANTS     
Sbjct: 361 NMRDALYRALPTSIKIALRSRLRAVDAREEPTYYDVKTEMDKILEWLVPIAANTS----- 420

Query: 425 RAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILLHHLIH 484
           +AHQ+CGRIGEWATQSKEHSKGRATQ+NN  RLQTLY+ADKVKTE QILELV LLHHLIH
Sbjct: 421 KAHQSCGRIGEWATQSKEHSKGRATQNNNANRLQTLYYADKVKTELQILELVTLLHHLIH 480

Query: 485 LAKRQQRRSTSLRCRSPTPKDMANNARRIQFKSQIIKANKDGVPAENRPLTGQSPTRKRD 544
           LAK QQRRS+SLRCRSPTPKDMAN +RRIQFKSQII+  KDG P +N P  GQ+P RK+ 
Sbjct: 481 LAKHQQRRSSSLRCRSPTPKDMANTSRRIQFKSQIIRTTKDGFPTDNIPSPGQTPIRKKV 540

BLAST of Lsi08G013130 vs. ExPASy TrEMBL
Match: A0A6J1K3J2 (uncharacterized protein LOC111492046 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492046 PE=4 SV=1)

HSP 1 Score: 763.1 bits (1969), Expect = 8.0e-217
Identity = 433/593 (73.02%), Postives = 467/593 (78.75%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGI KD F SEKITQ SEDR GNSCLN EA D NEMPQ+SRSGV LLPSP
Sbjct: 1   MGNTMGGVCSNGIDKDYFESEKITQTSEDRIGNSCLNSEAIDRNEMPQRSRSGVSLLPSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSK G+NKVAP+N+Q+G+RGRA+DL KTIG SVSNLHMN G FTGM SNG EISILAFEV
Sbjct: 61  PSKAGSNKVAPVNSQAGSRGRAIDLLKTIGNSVSNLHMNGGFFTGMASNGWEISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKV  LSQSLSEENIQLLK+E+LQSEGIKQL+STS EELLSIAAADKRQEFDV+ R
Sbjct: 121 ANTISKVVNLSQSLSEENIQLLKEELLQSEGIKQLVSTSSEELLSIAAADKRQEFDVLLR 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           EVIRFGKQCKD QWHNLDQYFSRLD NDSS+KQA EARA ++EL VLAQ TSELYHEL A
Sbjct: 181 EVIRFGKQCKDPQWHNLDQYFSRLDLNDSSKKQAREARAAIQELAVLAQSTSELYHELLA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W------LAAG 300
           LERFEQDYRRKVDEVE LNQ GIG                         W      +   
Sbjct: 241 LERFEQDYRRKVDEVESLNQGGIGESLSIFQGELNVQRKLVRSFQSKCLWSRSLDEIVEK 300

Query: 301 IQLLIS--NSLLYK------------PTIADKTLLTKDRSNGQKLGAVGLALHYANIISQ 360
           + ++++  N  + K            P I+DKTL  +DRS GQKLG+VGLALHYANIISQ
Sbjct: 301 LVIVVTWINQTIAKAFGDHNTGACNGPLISDKTLPIEDRSIGQKLGSVGLALHYANIISQ 360

Query: 361 INLIACRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWF 420
           INLIACRP SIPSNMRDALYRALPTSVKI LRSRLR VD SEE TY+ VKAEMDKILEW 
Sbjct: 361 INLIACRPASIPSNMRDALYRALPTSVKIGLRSRLRTVDVSEEPTYIGVKAEMDKILEWL 420

Query: 421 VPIAANTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQ 480
           VPIAANTS     +AHQACGRIGEWATQSKEHSKGRATQS NPIRLQTLYHAD+VKTEQQ
Sbjct: 421 VPIAANTS-----KAHQACGRIGEWATQSKEHSKGRATQS-NPIRLQTLYHADRVKTEQQ 480

Query: 481 ILELVILLHHLIHLAKRQQRRSTSLRCRSPTPKDMA----NNARRIQFKSQIIKANKDGV 540
           ILELV LLHHLIHL+K+Q +R TSLRC+SPTPK+MA     NARRIQFKSQIIKAN DG+
Sbjct: 481 ILELVTLLHHLIHLSKQQPQRFTSLRCQSPTPKNMAVPQPYNARRIQFKSQIIKANNDGI 540

Query: 541 PAENRPLTGQSPTRKRDLSNNKGMESYKNEN--KGIWTLSKAVSVSTLTSLGR 543
                       TRKRD SNNKG ESYKNEN  KGIWTLSK  SVSTL+SL R
Sbjct: 541 ---------TPTTRKRDPSNNKGTESYKNENIDKGIWTLSKGFSVSTLSSLAR 578

BLAST of Lsi08G013130 vs. ExPASy TrEMBL
Match: A0A6J1HF33 (uncharacterized protein LOC111462458 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462458 PE=4 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 4.4e-215
Identity = 431/594 (72.56%), Postives = 469/594 (78.96%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGI KD F SEKITQ SEDRKGNSCL+ EA DPNEMPQ+SRSGV LL SP
Sbjct: 1   MGNTMGGVCSNGIDKDYFESEKITQTSEDRKGNSCLSSEAIDPNEMPQRSRSGVTLLLSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSKTG+NKVAP+N+Q+G+RGRA+DL KTIG SVSNLHMNSG FTGM SNGREISILAFEV
Sbjct: 61  PSKTGSNKVAPVNSQAGSRGRAIDLLKTIGNSVSNLHMNSGFFTGMASNGREISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKVA LSQSLSEENIQLLK+E+LQSEGIKQL+STS EELLSIAAADKRQEFDV+  
Sbjct: 121 ANTISKVANLSQSLSEENIQLLKEELLQSEGIKQLVSTSSEELLSIAAADKRQEFDVLLG 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           EVIRFGKQCKD QWHNLDQYFSRLD NDSS+KQA EARA ++EL VLAQ TSELYHEL A
Sbjct: 181 EVIRFGKQCKDPQWHNLDQYFSRLDLNDSSKKQAREARAAIQELAVLAQSTSELYHELLA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W------LAAG 300
           LERFEQDYRRKVDEVE +NQAG G                         W      +   
Sbjct: 241 LERFEQDYRRKVDEVESMNQAGTGESLSIFQGELNVQRKLVRSFQSKCLWSRSLDEIVEK 300

Query: 301 IQLLIS--NSLLYK------------PTIADKTLLTKDRSNGQKLGAVGLALHYANIISQ 360
           + ++++  N  + K            P I DKTL  +DRSNGQKLG+VGLALHYA IISQ
Sbjct: 301 LVIVVTWINQTIAKTFDDHNTGACNGPVITDKTLPIEDRSNGQKLGSVGLALHYAIIISQ 360

Query: 361 INLIACRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWF 420
           INLIACRPTSIPSNMRDALYRALPTSVKI LRSRLR V+ SEE TY+ VKAEMDKILEW 
Sbjct: 361 INLIACRPTSIPSNMRDALYRALPTSVKIGLRSRLRNVNLSEEPTYIGVKAEMDKILEWL 420

Query: 421 VPIAANTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQ 480
           VPIAANTS     +AHQACGRIGEWATQSKEHSKGRATQS NPIRLQTLYHAD++KTEQQ
Sbjct: 421 VPIAANTS-----KAHQACGRIGEWATQSKEHSKGRATQS-NPIRLQTLYHADRIKTEQQ 480

Query: 481 ILELVILLHHLIHLAKRQQRRSTSLRCRSPTPKDMA----NNARRIQFKSQIIKANKDGV 540
           ILELV LLHH IHL K+Q +R TSLRC+SPT K+MA     NARRIQFKSQIIKAN DG+
Sbjct: 481 ILELVTLLHHFIHLGKQQPQRFTSLRCQSPTSKNMAVPQPYNARRIQFKSQIIKANNDGI 540

Query: 541 PAENRPLTGQSPT-RKRDLSNNKGMESYKNEN--KGIWTLSKAVSVSTLTSLGR 543
                     +PT RKRD SNNKG ESY+N N  KGIWTLSK  SVSTL+SL R
Sbjct: 541 ----------TPTIRKRDPSNNKGTESYRNYNKDKGIWTLSKGFSVSTLSSLAR 578

BLAST of Lsi08G013130 vs. ExPASy TrEMBL
Match: A0A6J1CSY9 (uncharacterized protein LOC111014009 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014009 PE=4 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 9.5e-202
Identity = 406/568 (71.48%), Postives = 446/568 (78.52%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQAS-EDRKGNSCLNFEASDPNEMPQKSRSGVILLPS 60
           MGNTMGGVCSNGI KDDFV EK T+ S +DRKGNSCL  EA+DP+ +PQKSRSGVILLPS
Sbjct: 1   MGNTMGGVCSNGIAKDDFVLEKKTEISDQDRKGNSCLISEATDPDYLPQKSRSGVILLPS 60

Query: 61  PPSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFE 120
           PPSKTG+NKVAPMN Q GARGRAV+L+KTIG SVSNLH N+G FTG  SNGREISILAFE
Sbjct: 61  PPSKTGSNKVAPMNPQGGARGRAVELFKTIGNSVSNLHTNTGFFTGTASNGREISILAFE 120

Query: 121 VANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVS 180
           VANTISK+A LSQSLSEE+IQ LKKE+ QSEGIKQL+ST++EELLSIAAADKRQEFD + 
Sbjct: 121 VANTISKLANLSQSLSEESIQFLKKELPQSEGIKQLVSTNMEELLSIAAADKRQEFDALL 180

Query: 181 REVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQ 240
           REVIRFGKQCKD QWHNLDQYFSRLD NDSSQKQA EARA L+ELTVLAQYTSELYHELQ
Sbjct: 181 REVIRFGKQCKDPQWHNLDQYFSRLDLNDSSQKQAREARAALQELTVLAQYTSELYHELQ 240

Query: 241 ALERFEQDYRRKVDEVEFLNQAGIGWLAA------GIQLLISNSLLYK------------ 300
           ALERFEQDYRRK+DEVE+LNQAGIG           +Q  +  SL  K            
Sbjct: 241 ALERFEQDYRRKLDEVEYLNQAGIGESLTIFQGELNVQRKLVRSLQSKCLWSRNLDEIVG 300

Query: 301 ----------PTIA--------DKTLLTKDRSNGQKLGAVGLALHYANIISQINLIACRP 360
                      TIA         +  L  DRS+GQKLG+VGLALHYANII+QINLIACRP
Sbjct: 301 KLVNIVTWIFQTIAKAFGDNSSGRPSLIVDRSSGQKLGSVGLALHYANIINQINLIACRP 360

Query: 361 TSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTS 420
           TSIPS+MRDALYRALPTSVK  LRSRL+AVDASEE TYL VKAEMD+ L+W VPIAANTS
Sbjct: 361 TSIPSSMRDALYRALPTSVKADLRSRLQAVDASEEPTYLSVKAEMDRTLQWLVPIAANTS 420

Query: 421 NMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILL 480
                +AHQACGRIGEWA+QSKE S+GRA Q NN IRLQTLYHADK KTEQ ILELV  L
Sbjct: 421 -----KAHQACGRIGEWASQSKEQSRGRAKQ-NNLIRLQTLYHADKEKTEQFILELVTSL 480

Query: 481 HHLIHLAKRQQRRSTSLRCRSPTPKDMANNARRIQFKSQIIKANKDGVPAENRPLTGQSP 529
           H +IHLAK+QQ+R TSLRCRSPTPKD+A   RRIQF+S II        AEN     Q+P
Sbjct: 481 HRIIHLAKQQQQRFTSLRCRSPTPKDLAKPQRRIQFRSHII--GTASASAEN-----QTP 540

BLAST of Lsi08G013130 vs. NCBI nr
Match: XP_038886160.1 (protein PSK SIMULATOR 2-like isoform X1 [Benincasa hispida] >XP_038886161.1 protein PSK SIMULATOR 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 852.8 bits (2202), Expect = 1.6e-243
Identity = 462/579 (79.79%), Postives = 494/579 (85.32%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGIVKDDFVSEKITQ SED KGNSC NFEASDPNEM QKS S VILLPSP
Sbjct: 1   MGNTMGGVCSNGIVKDDFVSEKITQTSEDGKGNSCSNFEASDPNEMLQKSCSSVILLPSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSKTG+NKVAP+NAQ+GARGRAVDLWKTIGIS+ NLHMN+G+FTGM +NGREISILAFEV
Sbjct: 61  PSKTGSNKVAPVNAQAGARGRAVDLWKTIGISLPNLHMNNGVFTGMATNGREISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKVA LSQSLSE NIQLLKKE+LQSEG+KQLISTSLEELLSIAAADKRQE DVVSR
Sbjct: 121 ANTISKVANLSQSLSEGNIQLLKKELLQSEGMKQLISTSLEELLSIAAADKRQELDVVSR 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           EVIRFG  CKDS+WHNLDQYFSRLD ND SQKQA EARA L ELTVL+Q TSELYHELQA
Sbjct: 181 EVIRFGNACKDSKWHNLDQYFSRLDLNDLSQKQAREARAALEELTVLSQNTSELYHELQA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W---LAAGIQL 300
           LERFEQDYRRKVDEVEFLNQAGIG                         W   L   ++ 
Sbjct: 241 LERFEQDYRRKVDEVEFLNQAGIGESLSIFQGELNVQRKLVRSFQSKCLWSRNLDEIVEK 300

Query: 301 LISNSLLYKPTIA--------DKTLLTKDRSNGQKLGAVGLALHYANIISQINLIACRPT 360
           L+        TIA        DKTLLT+DRSNGQKLGAVGLALHYANIISQINLIACRPT
Sbjct: 301 LVIVVTWINQTIAKAFGEDNTDKTLLTEDRSNGQKLGAVGLALHYANIISQINLIACRPT 360

Query: 361 SIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTSN 420
           SIPSNMRDALYRALPTSVKIALRSRLR V+ASEE TY DVKAE+DKILEW  PIAANT+ 
Sbjct: 361 SIPSNMRDALYRALPTSVKIALRSRLRPVNASEELTYFDVKAEVDKILEWLAPIAANTT- 420

Query: 421 MIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILLH 480
               +AHQ+CGRIGEWATQSKEHSKGRA Q+NNPIRLQTLYHADKVKTEQQILEL+ LLH
Sbjct: 421 ----KAHQSCGRIGEWATQSKEHSKGRAKQTNNPIRLQTLYHADKVKTEQQILELITLLH 480

Query: 481 HLIHLAKRQQRRSTSLRCRSPTPKDMANNARRIQFKSQIIKANKDGVPAENRPLTGQSPT 540
           HLIHLAK+QQ+RSTSLRCRSPTPKDMANN RRIQFKSQII++NKDGVPA +RP TGQ+P 
Sbjct: 481 HLIHLAKQQQQRSTSLRCRSPTPKDMANNVRRIQFKSQIIRSNKDGVPANSRPSTGQTPI 540

Query: 541 RKRDLSNNKGMESYKNENKGIWTLSKAVSVSTLTSLGRV 544
           RKR+LSNNKG ESYK+E KGIWTLSKAVSVST++SLGRV
Sbjct: 541 RKRELSNNKGTESYKSETKGIWTLSKAVSVSTVSSLGRV 574

BLAST of Lsi08G013130 vs. NCBI nr
Match: KAA0065159.1 (uncharacterized protein E6C27_scaffold82G004950 [Cucumis melo var. makuwa])

HSP 1 Score: 832.0 bits (2148), Expect = 2.9e-237
Identity = 455/579 (78.58%), Postives = 485/579 (83.77%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGIVKDDFVSEK+TQASEDRKGNS LNFEASDPNEMP+KS SGVILLPSP
Sbjct: 1   MGNTMGGVCSNGIVKDDFVSEKVTQASEDRKGNSHLNFEASDPNEMPEKSGSGVILLPSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSK G+NKVAPMNAQ+GARGRAVDLWKTIGISVSNLH+N+G FT MT +GREISILAFEV
Sbjct: 61  PSKAGSNKVAPMNAQAGARGRAVDLWKTIGISVSNLHINNGFFTAMTPSGREISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKVA LS+SLSEEN+QLLKKE+LQSEGIKQLIS SLEELLSIAAADKRQEF V+ R
Sbjct: 121 ANTISKVANLSKSLSEENMQLLKKELLQSEGIKQLISPSLEELLSIAAADKRQEFGVILR 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           EVIRFG QCKDS+WHNLDQYFSRLD NDSSQKQA EARA ++ELTVLAQ TSELYHELQA
Sbjct: 181 EVIRFGNQCKDSRWHNLDQYFSRLDSNDSSQKQAREARAAVQELTVLAQNTSELYHELQA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W---LAAGIQL 300
           LER EQDYRR+V+EVE LNQAGIG                         W   L   ++ 
Sbjct: 241 LERLEQDYRRRVEEVELLNQAGIGETLSIFQGELNVQRRLVRSFQSKCLWSRNLDEIVEK 300

Query: 301 LISNSLLYKPTI--------ADKTLLTKDRSNGQKLGAVGLALHYANIISQINLIACRPT 360
           L+        TI         DKTLL KDRSNGQKLGAVGLALHYANIISQINLIA RPT
Sbjct: 301 LVIVVTWINQTIVKEFGVDNTDKTLLIKDRSNGQKLGAVGLALHYANIISQINLIALRPT 360

Query: 361 SIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTSN 420
           SIPSNMRDALYRALPTS+KIALRSRLRAVDA EE TY DVKAEMDKILEW VPIAANTS 
Sbjct: 361 SIPSNMRDALYRALPTSIKIALRSRLRAVDAREEPTYYDVKAEMDKILEWLVPIAANTS- 420

Query: 421 MIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILLH 480
               +AHQ+CGRIGEWATQSKE SKGRATQSNNP RLQTLYHADKVKTE+QILELVILLH
Sbjct: 421 ----KAHQSCGRIGEWATQSKELSKGRATQSNNPNRLQTLYHADKVKTEKQILELVILLH 480

Query: 481 HLIHLAKRQQRRSTSLRCRSPTPKDMANNARRIQFKSQIIKANKDGVPAENRPLTGQSPT 540
           HLIH AK QQRRS+SLRCRSPTPKDMAN++RRIQFKSQII+  KDG P +N P   QSP 
Sbjct: 481 HLIHQAKHQQRRSSSLRCRSPTPKDMANSSRRIQFKSQIIRTTKDGFPTDNIPPASQSPI 540

Query: 541 RKRDLSNNKGMESYKNENKGIWTLSKAVSVSTLTSLGRV 544
           RKR L N KGMESYKNENKGIWTLSKAVSVSTL ++GRV
Sbjct: 541 RKRVLGNKKGMESYKNENKGIWTLSKAVSVSTLRTIGRV 574

BLAST of Lsi08G013130 vs. NCBI nr
Match: XP_011649665.1 (uncharacterized protein LOC101204577 isoform X1 [Cucumis sativus] >XP_031736338.1 uncharacterized protein LOC101204577 isoform X1 [Cucumis sativus] >KAE8652127.1 hypothetical protein Csa_022388 [Cucumis sativus])

HSP 1 Score: 825.9 bits (2132), Expect = 2.1e-235
Identity = 452/579 (78.07%), Postives = 480/579 (82.90%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGIVKDDFVSEKI QASEDRKGNS LN EA DPNEMP+KSRS VILLPSP
Sbjct: 1   MGNTMGGVCSNGIVKDDFVSEKIIQASEDRKGNSYLNSEARDPNEMPEKSRSDVILLPSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSKTG+NKVAPMNAQ+GARGRAVDLWKTIGISVSN H+NSG+ TGM  +GREISILAFEV
Sbjct: 61  PSKTGSNKVAPMNAQAGARGRAVDLWKTIGISVSNFHINSGVSTGMAPSGREISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKVA LS+SLSEENIQLLK E+LQSE IKQLIS SLEELLSIAAADKRQEF V+ R
Sbjct: 121 ANTISKVANLSKSLSEENIQLLKNELLQSEAIKQLISASLEELLSIAAADKRQEFGVILR 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           E+IRFG +CKDSQWHNLDQYFSRLD NDSSQKQA EARA L+ELTVLAQ TSELYHELQA
Sbjct: 181 EIIRFGNRCKDSQWHNLDQYFSRLDSNDSSQKQAREARAALQELTVLAQNTSELYHELQA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W---LAAGIQL 300
           LER EQDYRR+V+EVEFLNQAGIG                         W   L   ++ 
Sbjct: 241 LERLEQDYRRRVEEVEFLNQAGIGETLSIFQGELNVQRKLVRSFQSKCLWSRNLDEIVEK 300

Query: 301 LISNSLLYKPTI--------ADKTLLTKDRSNGQKLGAVGLALHYANIISQINLIACRPT 360
           L+        TI         DKTLL KDRSNGQKLGAVGLALHYANIISQINLIACRPT
Sbjct: 301 LVIVVTWINQTIIKEFGVDNTDKTLLIKDRSNGQKLGAVGLALHYANIISQINLIACRPT 360

Query: 361 SIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTSN 420
           SIPSNMRDALYRALPTS+KIALRSRLRAVDA EE TY DVK EMDKILEW VPIAANTS 
Sbjct: 361 SIPSNMRDALYRALPTSIKIALRSRLRAVDAREEPTYYDVKTEMDKILEWLVPIAANTS- 420

Query: 421 MIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILLH 480
               +AHQ+CGRIGEWATQSKEHSKGRATQ+NN  RLQTLY+ADKVKTE QILELV LLH
Sbjct: 421 ----KAHQSCGRIGEWATQSKEHSKGRATQNNNANRLQTLYYADKVKTELQILELVTLLH 480

Query: 481 HLIHLAKRQQRRSTSLRCRSPTPKDMANNARRIQFKSQIIKANKDGVPAENRPLTGQSPT 540
           HLIHLAK QQRRS+SLRCRSPTPKDMAN +RRIQFKSQII+  KDG P +N P  GQ+P 
Sbjct: 481 HLIHLAKHQQRRSSSLRCRSPTPKDMANTSRRIQFKSQIIRTTKDGFPTDNIPSPGQTPI 540

Query: 541 RKRDLSNNKGMESYKNENKGIWTLSKAVSVSTLTSLGRV 544
           RK+ L N KGMESYKNENKGIWTLSKAVSVSTL SLGRV
Sbjct: 541 RKKVLGNKKGMESYKNENKGIWTLSKAVSVSTLRSLGRV 574

BLAST of Lsi08G013130 vs. NCBI nr
Match: XP_022996976.1 (uncharacterized protein LOC111492046 isoform X1 [Cucurbita maxima] >XP_022996977.1 uncharacterized protein LOC111492046 isoform X1 [Cucurbita maxima])

HSP 1 Score: 763.1 bits (1969), Expect = 1.7e-216
Identity = 433/593 (73.02%), Postives = 467/593 (78.75%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGI KD F SEKITQ SEDR GNSCLN EA D NEMPQ+SRSGV LLPSP
Sbjct: 1   MGNTMGGVCSNGIDKDYFESEKITQTSEDRIGNSCLNSEAIDRNEMPQRSRSGVSLLPSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSK G+NKVAP+N+Q+G+RGRA+DL KTIG SVSNLHMN G FTGM SNG EISILAFEV
Sbjct: 61  PSKAGSNKVAPVNSQAGSRGRAIDLLKTIGNSVSNLHMNGGFFTGMASNGWEISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKV  LSQSLSEENIQLLK+E+LQSEGIKQL+STS EELLSIAAADKRQEFDV+ R
Sbjct: 121 ANTISKVVNLSQSLSEENIQLLKEELLQSEGIKQLVSTSSEELLSIAAADKRQEFDVLLR 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           EVIRFGKQCKD QWHNLDQYFSRLD NDSS+KQA EARA ++EL VLAQ TSELYHEL A
Sbjct: 181 EVIRFGKQCKDPQWHNLDQYFSRLDLNDSSKKQAREARAAIQELAVLAQSTSELYHELLA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W------LAAG 300
           LERFEQDYRRKVDEVE LNQ GIG                         W      +   
Sbjct: 241 LERFEQDYRRKVDEVESLNQGGIGESLSIFQGELNVQRKLVRSFQSKCLWSRSLDEIVEK 300

Query: 301 IQLLIS--NSLLYK------------PTIADKTLLTKDRSNGQKLGAVGLALHYANIISQ 360
           + ++++  N  + K            P I+DKTL  +DRS GQKLG+VGLALHYANIISQ
Sbjct: 301 LVIVVTWINQTIAKAFGDHNTGACNGPLISDKTLPIEDRSIGQKLGSVGLALHYANIISQ 360

Query: 361 INLIACRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWF 420
           INLIACRP SIPSNMRDALYRALPTSVKI LRSRLR VD SEE TY+ VKAEMDKILEW 
Sbjct: 361 INLIACRPASIPSNMRDALYRALPTSVKIGLRSRLRTVDVSEEPTYIGVKAEMDKILEWL 420

Query: 421 VPIAANTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQ 480
           VPIAANTS     +AHQACGRIGEWATQSKEHSKGRATQS NPIRLQTLYHAD+VKTEQQ
Sbjct: 421 VPIAANTS-----KAHQACGRIGEWATQSKEHSKGRATQS-NPIRLQTLYHADRVKTEQQ 480

Query: 481 ILELVILLHHLIHLAKRQQRRSTSLRCRSPTPKDMA----NNARRIQFKSQIIKANKDGV 540
           ILELV LLHHLIHL+K+Q +R TSLRC+SPTPK+MA     NARRIQFKSQIIKAN DG+
Sbjct: 481 ILELVTLLHHLIHLSKQQPQRFTSLRCQSPTPKNMAVPQPYNARRIQFKSQIIKANNDGI 540

Query: 541 PAENRPLTGQSPTRKRDLSNNKGMESYKNEN--KGIWTLSKAVSVSTLTSLGR 543
                       TRKRD SNNKG ESYKNEN  KGIWTLSK  SVSTL+SL R
Sbjct: 541 ---------TPTTRKRDPSNNKGTESYKNENIDKGIWTLSKGFSVSTLSSLAR 578

BLAST of Lsi08G013130 vs. NCBI nr
Match: XP_023546943.1 (uncharacterized protein LOC111805888 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 762.7 bits (1968), Expect = 2.2e-216
Identity = 433/594 (72.90%), Postives = 471/594 (79.29%), Query Frame = 0

Query: 1   MGNTMGGVCSNGIVKDDFVSEKITQASEDRKGNSCLNFEASDPNEMPQKSRSGVILLPSP 60
           MGNTMGGVCSNGI KD F SEKITQ SEDRKGNSCLN EA DPNEM Q+SRSG  LL SP
Sbjct: 1   MGNTMGGVCSNGIDKDYFESEKITQTSEDRKGNSCLNSEAIDPNEMQQRSRSGGTLLLSP 60

Query: 61  PSKTGNNKVAPMNAQSGARGRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEV 120
           PSKTG+NKVAP+N+Q+G+RGRA+DL KTIG SVSNLHMN G FTGM SNGREISILAFEV
Sbjct: 61  PSKTGSNKVAPVNSQAGSRGRAIDLLKTIGNSVSNLHMNGGFFTGMASNGREISILAFEV 120

Query: 121 ANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSR 180
           ANTISKVA LSQSLSEENIQLLK+E+LQSEGIKQL+STS EELLSIAAADKRQEFDV+ R
Sbjct: 121 ANTISKVANLSQSLSEENIQLLKEELLQSEGIKQLVSTSSEELLSIAAADKRQEFDVLLR 180

Query: 181 EVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQALEARATLRELTVLAQYTSELYHELQA 240
           EVIRFGKQCKD QWHNLDQYFSRLD NDSS+KQA EARA ++EL VLAQ TSELYHEL A
Sbjct: 181 EVIRFGKQCKDPQWHNLDQYFSRLDLNDSSKKQAREARAAIQELAVLAQSTSELYHELLA 240

Query: 241 LERFEQDYRRKVDEVEFLNQAGIG-------------------------W------LAAG 300
           LERFEQDYRRKVDEVE +NQAGIG                         W      +   
Sbjct: 241 LERFEQDYRRKVDEVESMNQAGIGESLSIFQGELNVQRKLVRSFQSKCLWSRSLDEIVEK 300

Query: 301 IQLLIS--NSLLYK------------PTIADKTLLTKDRSNGQKLGAVGLALHYANIISQ 360
           + ++++  N  + K            P I DKTL  +DRSNGQKLG+VGLALHYANIISQ
Sbjct: 301 LVIVVTWINQTIAKTFDDHNTGACNGPVITDKTLPIEDRSNGQKLGSVGLALHYANIISQ 360

Query: 361 INLIACRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWF 420
           INLIACRPTSIPSNMRDALYRALP SVKI LRSRLR V  SEE TY+ VKAEMDKILEW 
Sbjct: 361 INLIACRPTSIPSNMRDALYRALPISVKIGLRSRLRTVHVSEEPTYIGVKAEMDKILEWL 420

Query: 421 VPIAANTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQ 480
           VPIAANTS     +AHQACGRIGEWATQSKEHSKGRATQS NPIRLQTL+HAD+VKTEQQ
Sbjct: 421 VPIAANTS-----KAHQACGRIGEWATQSKEHSKGRATQS-NPIRLQTLFHADRVKTEQQ 480

Query: 481 ILELVILLHHLIHLAKRQQRRSTSLRCRSPTPKDMA----NNARRIQFKSQIIKANKDGV 540
           ILELV LLHHLIHLAK+Q +R TSLRC+SPTPK+MA     NARRIQFKSQIIKAN DG+
Sbjct: 481 ILELVTLLHHLIHLAKQQPQRFTSLRCQSPTPKNMAVPQPYNARRIQFKSQIIKANNDGI 540

Query: 541 PAENRPLTGQSPT-RKRDLSNNKGMESYKNE--NKGIWTLSKAVSVSTLTSLGR 543
                     +PT RKRD SNNKG ESY+N+  +KGIWTLSK  SVSTL+SL R
Sbjct: 541 ----------TPTIRKRDPSNNKGTESYRNDIKDKGIWTLSKGFSVSTLSSLAR 578

BLAST of Lsi08G013130 vs. TAIR 10
Match: AT1G30755.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 322.8 bits (826), Expect = 5.3e-88
Identity = 233/607 (38.39%), Postives = 333/607 (54.86%), Query Frame = 0

Query: 5   MGGVCSNGIVKDDFVSEKITQASEDR------KGNSCLNFEASDP-------NEMPQKSR 64
           MGGVCS  + KDD   +K+    +D+      K  S    + SD            + S+
Sbjct: 1   MGGVCS-CVFKDDDKKKKLRSNDDDKSRGFSGKLKSMRRSKTSDSYYSDNYGGSRRKSSK 60

Query: 65  SGVILL-------PSPPSKTGNNKVAPMNAQSGARG-----RAVDLWKTIGISVSNLHMN 124
              ++        P PP +  + K    N+  G  G     +AV++  T+G S++ ++ +
Sbjct: 61  PDEVVFNFSGELGPMPPLRNDSTKFMQRNSFMGRAGVMGLEKAVEVLDTLGSSMTRMNPS 120

Query: 125 SGLFTGMTSN-GREISILAFEVANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLIST 184
           +   +G+TS+ G +++ILAFEVANTI+K A L QSLSEEN++ +KK++L SE +K+L+ST
Sbjct: 121 NAYLSGVTSSRGGKVTILAFEVANTIAKGAALLQSLSEENLKFMKKDMLHSEEVKKLVST 180

Query: 185 SLEELLSIAAADKRQEFDVVSREVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQAL-EA 244
              EL  +AA+DKR+E D+ S EVIRFG  CKD QWHNLD+YF +LD  +S  K    +A
Sbjct: 181 DTTELQILAASDKREELDLFSGEVIRFGNMCKDLQWHNLDRYFMKLDTENSQHKLLKDDA 240

Query: 245 RATLRELTVLAQYTSELYHELQALERFEQDYRRKVDEVEFLNQAGIGWLAAGIQLL---- 304
            A ++EL  LA+ TSELYHELQAL+RFEQDYRRK+ EVE LN    G    GI +L    
Sbjct: 241 EARMQELVTLARITSELYHELQALDRFEQDYRRKLAEVESLNLPRRG---EGIVILQNEL 300

Query: 305 ---------ISNSLLYKPTIAD-------------KTLLTKDRSNG----------QKLG 364
                    +    L+   +A+             +T++    +NG          ++LG
Sbjct: 301 KQQKKLVKSLQKKSLWSQNLAEIIEKLVDVVSYIRQTIVEVFGNNGLRDNEGEQGRERLG 360

Query: 365 AVGLALHYANIISQINLIACRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTY 424
             GL+LHYAN+I QI+ IA RP+S+PSN+RD LY ALP +VK ALR RL+ +D  EE + 
Sbjct: 361 EAGLSLHYANLIQQIDNIASRPSSLPSNVRDTLYNALPATVKTALRPRLQTLDQEEELSV 420

Query: 425 LDVKAEMDKILEWFVPIAANTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSN--NPI 484
            ++KAEM+K L+W VP A NT+     +AHQ  G +GEWA    E  KG+    N  NP 
Sbjct: 421 PEIKAEMEKSLQWLVPFAENTT-----KAHQGFGWVGEWANSRIEFGKGKGKGENNGNPT 480

Query: 485 RLQTLYHADKVKTEQQILELVILLHHLIHLAKRQQRRSTSLRCRSPTPKDMANN--ARRI 541
           RLQTL+HADK   +  +LELV+ LH L+   K  ++R+  ++ +        NN      
Sbjct: 481 RLQTLHHADKPIVDSYVLELVVWLHRLM---KSSKKRAHGVKLQETNHVSPPNNRTISNT 540

BLAST of Lsi08G013130 vs. TAIR 10
Match: AT1G34320.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 293.1 bits (749), Expect = 4.5e-79
Identity = 200/522 (38.31%), Postives = 292/522 (55.94%), Query Frame = 0

Query: 14  VKDDFVSEKITQASEDRKGNS-CLNFEASDPNEMPQKSRSG------VILLPSPPSKTGN 73
           +KDD     +T+  +D K  S   +F        PQ    G      V+   S  +K+  
Sbjct: 43  LKDD--PSPVTENVDDNKHTSESFSFPIVSSGSHPQNIEDGIPRLSRVLSQKSRSTKSRQ 102

Query: 74  NKVAPMNAQSGARGR--------AVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAF 133
             VA ++  S   GR        AVD+  T+G S++NL+++ G  +  T  G +ISIL+F
Sbjct: 103 AAVAKVSEVSSLLGRAGTMGLGKAVDVLDTLGSSMTNLNLSGGFSSATTVKGNKISILSF 162

Query: 134 EVANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVV 193
           EVANTI K A L  SLS+++I  LK+ VL SEG++ LIS  ++ELL IAAADKR+E  + 
Sbjct: 163 EVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDELLRIAAADKREELRIF 222

Query: 194 SREVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQKQ-ALEARATLRELTVLAQYTSELYHE 253
           S EV+RFG +CKD Q+HNLD++F RL    + QK    EA   + ++     +T++LYHE
Sbjct: 223 SGEVVRFGNRCKDPQYHNLDRFFDRLGSEFTPQKHLKQEAETIMHQMMSFVHFTADLYHE 282

Query: 254 LQALERFEQDYRRKVDEVE--FLNQAGIGWLAAGIQLLISNSLLYKPTIADKTLLTK--- 313
           L AL+RFEQDY+RK+ E E     Q G+G   A ++  + +   +   +  K+L ++   
Sbjct: 283 LHALDRFEQDYQRKIQEEENPSTAQRGVGDTLAILRTELKSQKKHVRNLKKKSLWSRILE 342

Query: 314 --------------------------DRS------NGQKLGAVGLALHYANIISQINLIA 373
                                     D+       N +KLG+ GLALHYANII+QI+ + 
Sbjct: 343 EVMEKLVDVVHFLHLEIHEAFGGADPDKPANDPPINHKKLGSAGLALHYANIITQIDTLV 402

Query: 374 CRPTSIPSNMRDALYRALPTSVKIALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAA 433
            R +++P++ RDALY+ LP S+K ALRSR+++    EE T   +KAEM+K L+W VP+A 
Sbjct: 403 SRSSTMPASTRDALYQGLPPSIKSALRSRIQSFQVKEELTVPQIKAEMEKTLQWLVPVAT 462

Query: 434 NTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELV 482
           NT+     +AH   G +GEWA+   E ++  A Q+   +R+ TL+HADK KTE  IL+LV
Sbjct: 463 NTT-----KAHHGFGWVGEWASSGSEANQRPAGQT--ILRIDTLHHADKEKTEAYILDLV 522

BLAST of Lsi08G013130 vs. TAIR 10
Match: AT5G08660.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 251.9 bits (642), Expect = 1.2e-66
Identity = 159/412 (38.59%), Postives = 231/412 (56.07%), Query Frame = 0

Query: 80  GRAVDLWKTIGISVSNLHMNSGLFTGMTSNGREISILAFEVANTISKVAKLSQSLSEENI 139
           GRA D+  T+G S+++L  + G  +G+ + G E+ ILAFEVANTI K + L +SLS+ NI
Sbjct: 117 GRAKDVLDTLGSSMTDL-SSGGFTSGVATKGNELGILAFEVANTIVKSSNLIESLSKRNI 176

Query: 140 QLLKKEVLQSEGIKQLISTSLEELLSIAAADKRQEFDVVSREVIRFGKQCKDSQWHNLDQ 199
           + LK  +L SEG++ L+S   +ELL + AADKRQE  V S EV+RFG + KD QWHNL +
Sbjct: 177 EHLKGTILYSEGVQNLVSNDFDELLRLVAADKRQELQVFSGEVVRFGNRSKDFQWHNLQR 236

Query: 200 YFSRLDFNDSSQKQALE-ARATLRELTVLAQYTSELYHELQALERFEQDYRRKVDEVEFL 259
           YF R+    + Q+Q  E A   + +L VL QYT+ELY ELQ L R E+DY +K  E E  
Sbjct: 237 YFDRISKELTPQRQLKEDAVLVVDQLMVLVQYTAELYQELQVLYRLEKDYEQKRREEENS 296

Query: 260 NQAGIGWLAAGIQLLISNSLLYKPTIADKTLLTKD------------------------- 319
             +  G   A ++  +        ++  K+L ++                          
Sbjct: 297 ANSSKGDGLAILKTELKAQRKVVKSLKKKSLWSRGFEEVMEKLVDIVHFLLLEIHNIFGG 356

Query: 320 ----------RSNGQKLGAVGLALHYANIISQINLIACRPTSIPSNMRDALYRALPTSVK 379
                         ++LG  GLALHYANII QI+ +  R +SI SN RD+LY++LP  +K
Sbjct: 357 ADDQPSKKGAAEYDKRLGPAGLALHYANIIVQIDTLVARASSITSNARDSLYQSLPPGIK 416

Query: 380 IALRSRLRAVDASEESTYLDVKAEMDKILEWFVPIAANTSNMIRDRAHQACGRIGEWATQ 439
           +ALRS++++ +  +E +   +K EM++ L W VP+A NT+     +AH   G +GEWA  
Sbjct: 417 LALRSKIKSFNVDKELSVTQIKDEMERTLHWLVPVAGNTT-----KAHHGFGWVGEWANT 476

Query: 440 SKEHSKGRATQSNNPIRLQTLYHADKVKTEQQILELVILLHHLIHLAKRQQR 456
             + +        + +R++TLYHA K KTE  IL  +I L HL+  AK   R
Sbjct: 477 GTDFTS--KPSGGDILRIETLYHASKEKTEIYILGQIIWLQHLVTKAKSDAR 520

BLAST of Lsi08G013130 vs. TAIR 10
Match: AT3G23160.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-15
Identity = 111/488 (22.75%), Postives = 181/488 (37.09%), Query Frame = 0

Query: 113 ISILAFEVANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLISTSLEELLSIAAADKR 172
           I IL+FEVAN +SK   L +SLS+  I  LK EV  SEG+++L+S+    LL ++ ++K 
Sbjct: 43  IGILSFEVANVMSKTIHLHRSLSDTEISKLKAEVFHSEGVRKLVSSDENHLLDLSVSEKL 102

Query: 173 QEFDVVSREVIRFGKQCKDSQWHNLDQYF-----SRLDFN---------DSSQKQ----- 232
            +   V+  V R GK+C +      +  +       +DF          +S  K+     
Sbjct: 103 DDLSRVASVVSRLGKKCNEPALQGFEHVYEDIVNGAIDFRKLGFLVKDMESMVKKMERFV 162

Query: 233 --------ALEARATLRELTVLAQYTSELYHELQALER-----------------FEQDY 292
                    +E    L +  V  Q + +    ++A E+                 + Q Y
Sbjct: 163 NATCSLYCEMEVMNELEQAIVKLQRSQQHQESVKAFEQKLMWQRQDVKSLRDGSLWNQTY 222

Query: 293 RRKVD------------------------------------------------------- 352
            + V+                                                       
Sbjct: 223 DKVVEMLARTVCTIYGRIETVFGGLGLRGKKDVTLKRDRSKNEASKAVNSRSVAGFKDSR 282

Query: 353 --EVEFLNQAGIGWLAAGIQ--LLISNSLLYKPTIAD----------------------- 412
             E +   +AG      G     +    L    TI D                       
Sbjct: 283 RSEADEFTRAGDFNFPCGTNPGRMFMECLAMNRTIGDDDDDDDEDGGRITFPLSTARMIR 342

Query: 413 ------KTLLTKDRSNGQKLGAVGLALHYANIISQINLIACRPTSIPSNMRDALYRALPT 465
                 K+ LT+  ++   +G   L+LHYAN++  +  +   P  I    RD LY+ LPT
Sbjct: 343 SNKFGFKSRLTQ-HASASTIGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPT 402

BLAST of Lsi08G013130 vs. TAIR 10
Match: AT5G51670.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 77.4 bits (189), Expect = 3.9e-14
Identity = 101/462 (21.86%), Postives = 185/462 (40.04%), Query Frame = 0

Query: 97  HMNSGLFTGMTSNGREISILAFEVANTISKVAKLSQSLSEENIQLLKKEVLQSEGIKQLI 156
           H +S   +  TS+   + +L+FEVA  ++K+  L+ SL++ N+   +   L  EG+ +++
Sbjct: 24  HRSSPPISTTTSS---VGVLSFEVARVMTKLLHLTHSLTDSNLLTPRDHSLSLEGLTKIV 83

Query: 157 STSLEELLSIAAADKRQEFDVVSREVIRFGKQCKDSQWHNLDQYFSRLDFNDSSQ----- 216
           +      LS+  A+        +  V R   +C  +   +  + F   +F D  +     
Sbjct: 84  NGDETFHLSLVCAELADSLAHAANSVSRLSNRCTTASLRSFHRLFH--EFADMGRDPHGW 143

Query: 217 -KQALEARATLRELTVLAQYTSELYHELQALE-----------------RFEQDYRRKVD 276
                +  A  +++      T+ LY E++ +                    E+DY  K D
Sbjct: 144 VMNCKDTEAKNKKIERYVSVTTALYREMEEMAILENSLRKQSLQIGIEFEEEEDYENKKD 203

Query: 277 EVE-------------------------------------------------FLNQAGIG 336
            ++                                                 F + A  G
Sbjct: 204 VMKVIDLQNKIERQKQHVKYLKDRSLWNKSFDTVVLILARSVFTALARLKSVFSSAAATG 263

Query: 337 WLAAGIQLLISNSL--------LYKPTIADK--------TLLTKDRSNGQK-----LGAV 396
           ++   +   +  SL        L  P+  D+        +   ++ S   K     LG  
Sbjct: 264 YMVPTVVSSLPRSLSSSSSSMNLVHPSPNDEERDKTTTSSAFLEESSRLLKPPETTLGGA 323

Query: 397 GLALHYANIISQINLIACRPTSIPSNMRDALYRALPTSVKIALRSRLRAV--DASEESTY 456
           G+ALHYAN+I  +  +  +P  +  + RD LY  LP SV+ +LRSRL+ V   A++    
Sbjct: 324 GVALHYANLIVVMEKMIKQPQLVGLDARDDLYSMLPASVRSSLRSRLKGVGFTATDGGLA 383

Query: 457 LDVKAEMDKILEWFVPIAANTSNMIRDRAHQACGRIGEWATQSKEHSKGRATQSNNPIRL 463
            + KA + +IL W +P+A    NMIR ++ ++           ++H        N  + +
Sbjct: 384 TEWKAALGRILRWLLPLA---QNMIRWQSERSF---------EQQHMATATNSQNRVMLV 443