Lsi08G012970 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G012970 vs. ExPASy Swiss-Prot
Match: Q94CH8 (GDSL esterase/lipase EXL1 OS=Arabidopsis thaliana OX=3702 GN=EXL1 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 5.7e-82
Identity = 156/291 (53.61%), Postives = 204/291 (70.10%), Query Frame = 0

Query: 15  SFKLNVFQCLQLLLILQPAS---AAIKIPPGYSVPAVFVFGDSIVDTGNNNDLITQAKCN 74
           S  L +F C+ +L++L   S   A +KIP   +VPAV VFGDSIVD GNN+D+IT+A+C+
Sbjct: 17  SSSLILFWCIFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMITEARCD 76

Query: 75  YPPYGRDFPGGRPTGRFSNGRVPSDLV----GIKAFLPPYVNTNLQPEDLITGVNFASGG 134
           Y PYG DF GG  TGRFSNG+VP D+V    GIK  +P Y N NL+PE+L+TGV FASGG
Sbjct: 77  YAPYGIDFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGG 136

Query: 135 AGFDPLTSKTA-PAISLEAQLAMFREYKKKLEGLVGEERAKFIIDNSLYLVVAGSNDIAN 194
           AG+ PLT+K A   I L  QL  F EY +KL+ +VGE+R KFII NSL++V+ GSNDIAN
Sbjct: 137 AGYVPLTTKIAVGGIPLPQQLIYFEEYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIAN 196

Query: 195 TFYLARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRRIGFFGTPPLGCVPSQRTLAGG 254
            F+     ++HY + ++T  M  +A +F + LY  G RRI  FG PP+GCVPSQRT+AGG
Sbjct: 197 DFFTLPPVRLHYTVASFTALMADNARSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGG 256

Query: 255 IERACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYVDIYNPLLDVIQN 298
             R CV  +N A++LFN KL + +D L   L D  ++Y+DIY+PLLD+I N
Sbjct: 257 PTRDCVARFNDAAKLFNTKLSANIDVLSRTLQDPTIIYIDIYSPLLDLILN 307

BLAST of Lsi08G012970 vs. ExPASy Swiss-Prot
Match: Q94CH6 (GDSL esterase/lipase EXL3 OS=Arabidopsis thaliana OX=3702 GN=EXL3 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 1.1e-80
Identity = 151/288 (52.43%), Postives = 204/288 (70.83%), Query Frame = 0

Query: 14  SSFKLNVFQCLQLLLILQPASAAIKIPPGYSVPAVFVFGDSIVDTGNNNDLITQAKCNYP 73
           SS+K+    CL  +L L     A+K+PP   +PAV  FGDSIVDTG NN++ T  KC++ 
Sbjct: 14  SSWKI----CLLSVLFLTETITAVKLPPKLIIPAVIAFGDSIVDTGMNNNVKTVVKCDFL 73

Query: 74  PYGRDFPGGRPTGRFSNGRVPSDL----VGIKAFLPPYVNTNLQPEDLITGVNFASGGAG 133
           PYG +F  G  TGRF +GRVP+DL    +GIK+ +P Y++ NL+ +DL+TGV+FASGG+G
Sbjct: 74  PYGINFQSGVATGRFCDGRVPADLLAEELGIKSIVPAYLDPNLKSKDLLTGVSFASGGSG 133

Query: 134 FDPLTSKTAPAISLEAQLAMFREYKKKLEGLVGEERAKFIIDNSLYLVVAGSNDIANTFY 193
           +DP+T K    ISLE QL+ F EY +K++ +VGE R  FI+ NSL+L+VAGS+DIANT+Y
Sbjct: 134 YDPITPKLVAVISLEDQLSYFEEYIEKVKNIVGEARKDFIVANSLFLLVAGSDDIANTYY 193

Query: 194 LARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRRIGFFGTPPLGCVPSQRTLAGGIER 253
             R R   Y++++YT  M  SAS FV +LY  G RR+  FG PP+GCVPSQRTL GGI R
Sbjct: 194 TLRARP-EYDVDSYTTLMSDSASEFVTKLYGYGVRRVAVFGAPPIGCVPSQRTLGGGILR 253

Query: 254 ACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYVDIYNPLLDVIQN 298
            C + YN+A++LFN KL   LD L+  LP  + +Y++IY+PL D+IQN
Sbjct: 254 DCADNYNEAAKLFNSKLSPKLDSLRKTLPGIKPIYINIYDPLFDIIQN 296

BLAST of Lsi08G012970 vs. ExPASy Swiss-Prot
Match: Q9FHW9 (GDSL esterase/lipase At5g42170 OS=Arabidopsis thaliana OX=3702 GN=At5g42170/At5g42160 PE=3 SV=2)

HSP 1 Score: 291.6 bits (745), Expect = 2.5e-77
Identity = 142/265 (53.58%), Postives = 185/265 (69.81%), Query Frame = 0

Query: 37  IKIPPGYSVPAVFVFGDSIVDTGNNNDLITQAKCNYPPYGRDFPGGRPTGRFSNGRVPSD 96
           IK+PP  ++P +  FGDSIVD+GNNN L T  KCN+PPYG+DFPG   TGRFS+GRVPSD
Sbjct: 40  IKLPPNVTIPGIITFGDSIVDSGNNNHLRTALKCNFPPYGKDFPGKIATGRFSDGRVPSD 99

Query: 97  LV----GIKAFLPPYVNTNLQPEDLITGVNFASGGAGFDPLTSKTAPAISLEAQLAMFRE 156
           +V    GI   +P Y+N  L+ EDL+ GVNFASGG+G+DPLT+K    +SL  QL  F+E
Sbjct: 100 IVAERLGIAETIPAYLNPKLKNEDLLKGVNFASGGSGYDPLTAKLVKVVSLSDQLKNFQE 159

Query: 157 YKKKLEGLVGEERAKFIIDNSLYLVVAGSNDIANTFYLARIRQVHYNINTYTDFMVQSAS 216
           YK KL+ +VGEE+A F++ NSLYLVVA SNDIA+T+     R + YN  +Y D++  SAS
Sbjct: 160 YKNKLKVIVGEEKANFLVKNSLYLVVASSNDIAHTY---TARSIKYNKTSYADYLADSAS 219

Query: 217 TFVKELYAAGGRRIGFFGTPPLGCVPSQRTLAGGIERACVNEYNKASELFNGKLHSTLDY 276
            FV  LY  G RRIG F   P+GCVP+ RTL G ++R C  + N+ +  FN K+  TL+ 
Sbjct: 220 KFVSALYGLGARRIGVFSAVPVGCVPAARTLRGKLKRRCSEKLNEVARNFNAKISPTLEA 279

Query: 277 LQTILPDSRVVYVDIYNPLLDVIQN 298
           L   LPDSRVV +D+ + L D+I+N
Sbjct: 280 LGKELPDSRVVLIDVCDTLNDMIEN 301

BLAST of Lsi08G012970 vs. ExPASy Swiss-Prot
Match: Q94CH7 (GDSL esterase/lipase EXL2 OS=Arabidopsis thaliana OX=3702 GN=EXL2 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 4.0e-75
Identity = 147/302 (48.68%), Postives = 199/302 (65.89%), Query Frame = 0

Query: 14  SSFKLNVFQCLQLLLILQPAS--AAIKIPPGYSVPAVFVFGDSIVDTGNNNDLITQ-AKC 73
           +SF  + F C+  L++L   S  A +K PP  + PA+ VFGDSIVD GNN+D++T  A+C
Sbjct: 12  TSFSSSPFWCVFFLVLLCKTSTNALVKQPPNETTPAIIVFGDSIVDAGNNDDIMTTLARC 71

Query: 74  NYPPYGRDFPGGRPTGRFSNGRVPSDLV----GIKAFLPPYVNTNLQPEDLITGVNFASG 133
           NYPPYG DF GG PTGRF NG+V +D +    GIK  +P Y N NL+PEDL+TGV FASG
Sbjct: 72  NYPPYGIDFDGGIPTGRFCNGKVATDFIAGKFGIKPSIPAYRNPNLKPEDLLTGVTFASG 131

Query: 134 GAGFDPLTSKTAP-------------AISLEAQLAMFREYKKKLEGLVGEERAKFIIDNS 193
           GAG+ P T++ +               I+L  QL +F EY +K++ +VGEER K II NS
Sbjct: 132 GAGYVPFTTQLSTYLFIYKPLLFLKGGIALSQQLKLFEEYVEKMKKMVGEERTKLIIKNS 191

Query: 194 LYLVVAGSNDIANTFYLARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRRIGFFGTPP 253
           L++V+ GSNDI NT++     Q  Y++ ++T  M  +A +F ++L+  G RRI  FG PP
Sbjct: 192 LFMVICGSNDITNTYFGLPSVQQQYDVASFTTLMADNARSFAQKLHEYGARRIQVFGAPP 251

Query: 254 LGCVPSQRTLAGGIERACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYVDIYNPLLD 296
           +GCVPSQRTLAGG  R CV  +N A++L+N KL + L  L   L D  ++YVDIY+ LLD
Sbjct: 252 VGCVPSQRTLAGGPTRNCVVRFNDATKLYNVKLAANLGSLSRTLGDKTIIYVDIYDSLLD 311

BLAST of Lsi08G012970 vs. ExPASy Swiss-Prot
Match: Q9LH73 (GDSL esterase/lipase At3g14820 OS=Arabidopsis thaliana OX=3702 GN=At3g14820 PE=3 SV=2)

HSP 1 Score: 279.3 bits (713), Expect = 1.3e-73
Identity = 133/258 (51.55%), Postives = 187/258 (72.48%), Query Frame = 0

Query: 44  SVPAVFVFGDSIVDTGNNNDLITQAKCNYPPYGRDFPGGRPTGRFSNGRVPSDLV----G 103
           ++PA+ VFGDSI+DTGNNND+ T  K N+PPYGRDFPG  PTGRFS+G+VPSD++    G
Sbjct: 29  TIPALIVFGDSIMDTGNNNDIPTLLKSNFPPYGRDFPGAIPTGRFSDGKVPSDIIAESLG 88

Query: 104 IKAFLPPYVNTNLQPEDLITGVNFASGGAGFDPLTSKTAPAISLEAQLAMFREYKKKLEG 163
           I   LPPY+ +NL+P DL+ GV FASGG+G+DPLTS     +S+  QL  F+EY  K++ 
Sbjct: 89  IAKTLPPYLGSNLKPHDLLKGVIFASGGSGYDPLTSTLLSVVSMSDQLKYFQEYLAKIKQ 148

Query: 164 LVGEERAKFIIDNSLYLVVAGSNDIANTFYLARIRQVHYNINTYTDFMVQSASTFVKELY 223
             GEE+ KFI++ S++LVV+ SND+A T++   +R V Y+ N+Y +++V+ AS F+KEL 
Sbjct: 149 HFGEEKVKFILEKSVFLVVSSSNDLAETYW---VRSVEYDRNSYAEYLVELASEFIKELS 208

Query: 224 AAGGRRIGFFGTPPLGCVPSQRTLAGGIERACVNEYNKASELFNGKLHSTLDYLQTILPD 283
             G + IG F   P+GC+P+QRTL GG ER C  + N  +  FN KL S+LD L+  LP 
Sbjct: 209 ELGAKNIGLFSGVPVGCLPAQRTLFGGFERKCYEKLNNMALHFNSKLSSSLDTLKKELP- 268

Query: 284 SRVVYVDIYNPLLDVIQN 298
           SR++++D+Y+ LLD+I+N
Sbjct: 269 SRLIFIDVYDTLLDIIKN 282

BLAST of Lsi08G012970 vs. ExPASy TrEMBL
Match: A0A5J5ADW3 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_036125 PE=3 SV=1)

HSP 1 Score: 790.8 bits (2041), Expect = 4.8e-225
Identity = 402/706 (56.94%), Postives = 515/706 (72.95%), Query Frame = 0

Query: 50  VFGDSIVDTGNNNDLITQAKCNYPPYGRDFPGGRPTGRFSNGRVPSDL----VGIKAFLP 109
           +FGDSIVDTGNNN+L T AK NYPPYG+DF GG+PTGRFSNG+VPSDL    +GIK  LP
Sbjct: 1   MFGDSIVDTGNNNNLKTVAKVNYPPYGKDFMGGKPTGRFSNGKVPSDLLVEELGIKELLP 60

Query: 110 PYVNTNLQPEDLITGVNFASGGAGFDPLTSKTAPAISLEAQLAMFREYKKKLEGLVGEER 169
           PY++ NLQ  DL TG+NFASG AG+DPLTS+    ISL  QL +F+EY  KL+G+VGEER
Sbjct: 61  PYLDPNLQTNDLSTGLNFASGAAGYDPLTSQLVSVISLSNQLELFKEYIVKLKGVVGEER 120

Query: 170 AKFIIDNSLYLVVAGSNDIANTFYLARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRR 229
              I+ NSLY VVAGSNDI+NT++    R++ Y++ +Y DFMV  ASTFV+ELY  G RR
Sbjct: 121 TSSILSNSLYTVVAGSNDISNTYFSTAFRKLQYDVPSYADFMVSMASTFVQELYKLGARR 180

Query: 230 IGFFGTPPLGCVPSQRTLAGGIERACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYV 289
           IG    PP+GCVPSQRT AGG ER CV +YN+A++LFN KL + L+ L+T LP +++VY+
Sbjct: 181 IGVMSVPPIGCVPSQRTGAGGPERECVEKYNEAAQLFNTKLSAELNSLKTNLPHAKIVYI 240

Query: 290 DIYNPLLDVIQN----------------------YVPPIMEPATFVVDKLKGFGTATQNF 349
           DI+NPLLD+IQN                      Y+   MEP   VVDKLKGF  + Q+F
Sbjct: 241 DIFNPLLDLIQNPEKYGFKIADKGCCGTGTIEVEYLCFEMEPGASVVDKLKGFSKSFQDF 300

Query: 350 FDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQDKVEKVLSFYNTQRSSPFQENA 409
            +G  HRR  S+RR+PIEILKRLQREAFSDLM+LRDRQDK+E++LSFY + + SPFQE +
Sbjct: 301 ANGFFHRRNNSNRRNPIEILKRLQREAFSDLMKLRDRQDKMERLLSFYKSSKGSPFQEVS 360

Query: 410 THVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSRLTFETTVRENDSLVAEFVANQ 469
           T V+GEV+ L ALL+M  +D  +++ +RRAGI  GI SR TFE+T+R+ND+LV EFVA++
Sbjct: 361 TRVRGEVDFLEALLMMDNIDQQNYDTIRRAGIRRGIQSRFTFESTIRQNDNLVVEFVASE 420

Query: 470 KGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGARCRDVAVIANPSHQEKGLTDTS 529
           +G+   G   GS L+L+KVFY+A + DW SA+ +PVGARCRDV V  N SHQ++GLT+ S
Sbjct: 421 EGQ---GDALGSPLSLAKVFYEANISDWFSAVAIPVGARCRDVGVATNSSHQKRGLTNYS 480

Query: 530 SFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISS-ERIQPSFDRIQHYFGTFGQLVCQLP 589
           SFGPPLL+ PNG A GL VRKSN  AS+A F+S    +QP    I   F TFGQ+VCQL 
Sbjct: 481 SFGPPLLNQPNGSAFGLMVRKSNFGASVAHFLSGLGNMQPGSVGITRCFSTFGQVVCQLS 540

Query: 590 RGTKLSVLGLLQATKLSNQHVNLGALTIPVCLSRHKSA--ETVEASDPRLLTVSGEAISR 649
           R TKLS+LG+ Q  KLS+Q VNLGAL +P+ + +H+ A   ++EAS   + +   E +  
Sbjct: 541 RSTKLSLLGIHQLPKLSSQQVNLGALALPIGIFKHRKAPETSMEASATPIESQLEENVMA 600

Query: 650 GSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNNNEDVLGWGMSLSGILGGPL 709
           GS+AL+LESELDE TRIGGW+E+ +S+PKYLQWAV+MSD   ED  GWG+SL G + GP 
Sbjct: 601 GSIALMLESELDESTRIGGWLEIKKSDPKYLQWAVTMSD-TPEDEFGWGLSLGGSIQGPK 660

Query: 710 DGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVRSNWSL 727
             D FQVE+++K N+ KRFSL+P + YV DG  +  A ++RS WSL
Sbjct: 661 SWDHFQVETFLKFNLGKRFSLQPALVYVMDGTTQFPALMLRSTWSL 702

BLAST of Lsi08G012970 vs. ExPASy TrEMBL
Match: A0A1S3BBY0 (uncharacterized protein LOC103488388 OS=Cucumis melo OX=3656 GN=LOC103488388 PE=4 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 2.5e-213
Identity = 383/425 (90.12%), Postives = 397/425 (93.41%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           MEP TFVVDKLKGFGTATQNFF GLVHRREKS+RRSPIEILKRLQREAFSDLMRLRDRQD
Sbjct: 1   MEPVTFVVDKLKGFGTATQNFFYGLVHRREKSARRSPIEILKRLQREAFSDLMRLRDRQD 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVEKVLS YNTQRSSPFQENATHVKGEVNILGALL MSV+DNHSF+AL RAGISTGIHSR
Sbjct: 61  KVEKVLSLYNTQRSSPFQENATHVKGEVNILGALLFMSVIDNHSFDALHRAGISTGIHSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
           LTFETTVRE+DSLVAEFVANQK KVDFGVDSGSELTLSKV YKA V DWMSA VVPVGAR
Sbjct: 121 LTFETTVRESDSLVAEFVANQKAKVDFGVDSGSELTLSKVLYKANVGDWMSATVVPVGAR 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
           CRDVAVIANPSHQEKGLTD SSFGPPL D PNGGAIGLTVRKSN+TASLAQFIS+ERIQP
Sbjct: 181 CRDVAVIANPSHQEKGLTDASSFGPPLFDQPNGGAIGLTVRKSNLTASLAQFISTERIQP 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKL-SNQHVNLGALTIPVCLSRHKSAE 602
           SFDRIQH+ GTFGQLVCQLPRGTKLS+LGLLQ  KL SNQHVNLGALTIPVCLSR KS E
Sbjct: 241 SFDRIQHHLGTFGQLVCQLPRGTKLSLLGLLQVPKLSSNQHVNLGALTIPVCLSRRKSPE 300

Query: 603 TVEASDPRLLTVSGEAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNN 662
           TVE  DPRLLTVSGEAISR S+ALLLESELDEVTRIGGWVELSQSN KYLQWAVSMSDNN
Sbjct: 301 TVEGPDPRLLTVSGEAISRRSIALLLESELDEVTRIGGWVELSQSNSKYLQWAVSMSDNN 360

Query: 663 NEDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVR 722
           +ED LGWGMSLSGILG  LD D FQVESYVKLN+SK+F+LKPGIAYVTDGNAKMMAF+VR
Sbjct: 361 SEDALGWGMSLSGILGSSLDRDHFQVESYVKLNVSKKFNLKPGIAYVTDGNAKMMAFLVR 420

Query: 723 SNWSL 727
           SNWSL
Sbjct: 421 SNWSL 425

BLAST of Lsi08G012970 vs. ExPASy TrEMBL
Match: A0A0A0LQH7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G346070 PE=4 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 7.0e-208
Identity = 373/426 (87.56%), Postives = 393/426 (92.25%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           MEP TFVVDKLKGFGTATQNFFDGLVHRREKS+RRSPIEILKRLQREAFSDLMRLRDRQD
Sbjct: 1   MEPVTFVVDKLKGFGTATQNFFDGLVHRREKSARRSPIEILKRLQREAFSDLMRLRDRQD 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVEKVLS YNTQRSSPFQEN THVKGEVN+LGALL MSV+DNHSF AL RAGISTGIHSR
Sbjct: 61  KVEKVLSLYNTQRSSPFQENVTHVKGEVNVLGALLFMSVIDNHSFKALHRAGISTGIHSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
           LTFETTVRE+DSLVAEFVANQK KVDFGVDSGSELTLSKV YKA V DWMSA VVPVGAR
Sbjct: 121 LTFETTVRESDSLVAEFVANQKAKVDFGVDSGSELTLSKVLYKASVGDWMSATVVPVGAR 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
           CRDV++IANPSHQEKGLTD SSFGPPLLD PNGGAIGLTVRKSN+TASLAQFIS+ERIQP
Sbjct: 181 CRDVSIIANPSHQEKGLTDASSFGPPLLDQPNGGAIGLTVRKSNLTASLAQFISTERIQP 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKL-SNQHVNLGALTIPVCLSRHKSAE 602
           SFDRIQH+ GTFGQLVCQLPRG KLS+LGLLQ  KL SNQHVNLGALTIPVCLSR KS E
Sbjct: 241 SFDRIQHHLGTFGQLVCQLPRGMKLSLLGLLQVPKLSSNQHVNLGALTIPVCLSRRKSPE 300

Query: 603 TVEASDPRLLTVSG-EAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDN 662
           TV+  DPRLLTVSG EAISR S+ALLLESELDE TRI GWVELSQSN KYL+WAVS+SDN
Sbjct: 301 TVDGPDPRLLTVSGEEAISRRSIALLLESELDEETRIAGWVELSQSNSKYLRWAVSVSDN 360

Query: 663 NNEDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVV 722
           N+ED LGWGMSLSGILG  +D D FQVESYVKLN+SK+F+LKPGIAYVTDGNAKMMAF+V
Sbjct: 361 NSEDALGWGMSLSGILGSAIDRDHFQVESYVKLNVSKKFNLKPGIAYVTDGNAKMMAFLV 420

Query: 723 RSNWSL 727
           RS WSL
Sbjct: 421 RSKWSL 426

BLAST of Lsi08G012970 vs. ExPASy TrEMBL
Match: A0A6J1CSD9 (uncharacterized protein LOC111013892 OS=Momordica charantia OX=3673 GN=LOC111013892 PE=4 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 1.6e-196
Identity = 360/425 (84.71%), Postives = 386/425 (90.82%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           MEP T+VVDKLKGFGTA+ NF DGLVHRREKSSRR PIEILKRLQREAFSDLMRLRDRQD
Sbjct: 1   MEPITYVVDKLKGFGTASHNFIDGLVHRREKSSRRRPIEILKRLQREAFSDLMRLRDRQD 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVEK LSFY TQ+SSPFQE+ATHVKGEV++LGALLLMSV+D +S NAL RAGISTGIHSR
Sbjct: 61  KVEKTLSFYGTQKSSPFQESATHVKGEVDLLGALLLMSVIDRNSCNALCRAGISTGIHSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
           LTFE TVRENDSLVAEFVANQK  VDFGV SGSELTLSKVFYKA V DWMSAI +P+GAR
Sbjct: 121 LTFEATVRENDSLVAEFVANQKAIVDFGVHSGSELTLSKVFYKASVGDWMSAIAIPLGAR 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
           CRDVAVIANPSHQEKGLTD SSFGPPLLD P+GGAIGLTVRKSNVTASLAQFIS+ERIQP
Sbjct: 181 CRDVAVIANPSHQEKGLTDISSFGPPLLDQPSGGAIGLTVRKSNVTASLAQFISTERIQP 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKLSNQHVNLGALTIPVCL-SRHKSAE 602
           SFDRIQHY  TFGQLV QLPRGTKLS+LGLLQA K + QHVNLGALT+PV L SRH S E
Sbjct: 241 SFDRIQHYLSTFGQLVYQLPRGTKLSLLGLLQAPKFT-QHVNLGALTLPVSLGSRHTSPE 300

Query: 603 TVEASDPRLLTVSGEAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNN 662
           TVEA DP   TVS +A+SRGS+ALLLESELDE+TRIGGWVE+SQSNPK++QWAVS+SD N
Sbjct: 301 TVEAPDPLSSTVSRKAMSRGSIALLLESELDELTRIGGWVEVSQSNPKHMQWAVSVSD-N 360

Query: 663 NEDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVR 722
           +ED LGWGMSLSGI  GPLD  QFQVESYVKLNMSKRFSLKPGIAYVTDG+AKMMAF+VR
Sbjct: 361 SEDALGWGMSLSGITQGPLDRSQFQVESYVKLNMSKRFSLKPGIAYVTDGDAKMMAFLVR 420

Query: 723 SNWSL 727
           SNWSL
Sbjct: 421 SNWSL 423

BLAST of Lsi08G012970 vs. ExPASy TrEMBL
Match: A0A6J1KDN4 (uncharacterized protein LOC111492305 OS=Cucurbita maxima OX=3661 GN=LOC111492305 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 4.9e-193
Identity = 352/421 (83.61%), Postives = 381/421 (90.50%), Query Frame = 0

Query: 309 VVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQDKVEKVL 368
           VVDKLKGFGTATQ+FFDGLVHRREKSSRRSPIEI+KRLQREAFSD+MR+RDRQDKVEK+L
Sbjct: 4   VVDKLKGFGTATQSFFDGLVHRREKSSRRSPIEIMKRLQREAFSDIMRIRDRQDKVEKIL 63

Query: 369 SFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSRLTFETT 428
           SFYNTQ+SSPFQENATHVKGEV+++GALLLMSV+DNHS NALRRAGISTGIHSRLTFETT
Sbjct: 64  SFYNTQKSSPFQENATHVKGEVHMVGALLLMSVIDNHSVNALRRAGISTGIHSRLTFETT 123

Query: 429 VRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGARCRDVAV 488
           VRE+DSLVAEFVANQK  VD GVDSG ELTLSKV YKA VRDWMSAIVVPVGARCRDVAV
Sbjct: 124 VRESDSLVAEFVANQKAVVDSGVDSGRELTLSKVLYKASVRDWMSAIVVPVGARCRDVAV 183

Query: 489 IANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQPSFDRIQ 548
           +ANPSHQEKGLTD SS+GPPLL+ PNGGAIGLTVRKSNVTASLAQFIS+ER QPS DRIQ
Sbjct: 184 LANPSHQEKGLTDISSYGPPLLEQPNGGAIGLTVRKSNVTASLAQFISTERNQPSLDRIQ 243

Query: 549 HYFGTFGQLVCQLPRGTKLSVLGLLQATKLSNQHVNLGALTIPVCL-SRHKSAETVEASD 608
           H  GTFGQLVCQLPRGTKLS+LGLL+  KLSN  +NLGALTIPVCL +RH+S ETVEA D
Sbjct: 244 HDLGTFGQLVCQLPRGTKLSLLGLLEVPKLSN--LNLGALTIPVCLGTRHESPETVEAPD 303

Query: 609 PRLLTVSGEAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSD--NNNEDV 668
           P      G+AISR S+AL LESELDE TRIGGWVEL+QSNPK ++WAVSMSD  NNNED 
Sbjct: 304 P------GKAISRRSIALHLESELDEATRIGGWVELNQSNPKQIKWAVSMSDKNNNNEDA 363

Query: 669 LGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVRSNWS 727
           LGWGMSLSGI GGP + DQF+ ESYV LNM+KRF LKPGIAYVTDGNAK MAF+VRSNWS
Sbjct: 364 LGWGMSLSGIAGGPQERDQFKAESYVTLNMNKRFKLKPGIAYVTDGNAKAMAFLVRSNWS 416

BLAST of Lsi08G012970 vs. NCBI nr
Match: KAA8528770.1 (hypothetical protein F0562_036125 [Nyssa sinensis])

HSP 1 Score: 790.8 bits (2041), Expect = 1.0e-224
Identity = 402/706 (56.94%), Postives = 515/706 (72.95%), Query Frame = 0

Query: 50  VFGDSIVDTGNNNDLITQAKCNYPPYGRDFPGGRPTGRFSNGRVPSDL----VGIKAFLP 109
           +FGDSIVDTGNNN+L T AK NYPPYG+DF GG+PTGRFSNG+VPSDL    +GIK  LP
Sbjct: 1   MFGDSIVDTGNNNNLKTVAKVNYPPYGKDFMGGKPTGRFSNGKVPSDLLVEELGIKELLP 60

Query: 110 PYVNTNLQPEDLITGVNFASGGAGFDPLTSKTAPAISLEAQLAMFREYKKKLEGLVGEER 169
           PY++ NLQ  DL TG+NFASG AG+DPLTS+    ISL  QL +F+EY  KL+G+VGEER
Sbjct: 61  PYLDPNLQTNDLSTGLNFASGAAGYDPLTSQLVSVISLSNQLELFKEYIVKLKGVVGEER 120

Query: 170 AKFIIDNSLYLVVAGSNDIANTFYLARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRR 229
              I+ NSLY VVAGSNDI+NT++    R++ Y++ +Y DFMV  ASTFV+ELY  G RR
Sbjct: 121 TSSILSNSLYTVVAGSNDISNTYFSTAFRKLQYDVPSYADFMVSMASTFVQELYKLGARR 180

Query: 230 IGFFGTPPLGCVPSQRTLAGGIERACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYV 289
           IG    PP+GCVPSQRT AGG ER CV +YN+A++LFN KL + L+ L+T LP +++VY+
Sbjct: 181 IGVMSVPPIGCVPSQRTGAGGPERECVEKYNEAAQLFNTKLSAELNSLKTNLPHAKIVYI 240

Query: 290 DIYNPLLDVIQN----------------------YVPPIMEPATFVVDKLKGFGTATQNF 349
           DI+NPLLD+IQN                      Y+   MEP   VVDKLKGF  + Q+F
Sbjct: 241 DIFNPLLDLIQNPEKYGFKIADKGCCGTGTIEVEYLCFEMEPGASVVDKLKGFSKSFQDF 300

Query: 350 FDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQDKVEKVLSFYNTQRSSPFQENA 409
            +G  HRR  S+RR+PIEILKRLQREAFSDLM+LRDRQDK+E++LSFY + + SPFQE +
Sbjct: 301 ANGFFHRRNNSNRRNPIEILKRLQREAFSDLMKLRDRQDKMERLLSFYKSSKGSPFQEVS 360

Query: 410 THVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSRLTFETTVRENDSLVAEFVANQ 469
           T V+GEV+ L ALL+M  +D  +++ +RRAGI  GI SR TFE+T+R+ND+LV EFVA++
Sbjct: 361 TRVRGEVDFLEALLMMDNIDQQNYDTIRRAGIRRGIQSRFTFESTIRQNDNLVVEFVASE 420

Query: 470 KGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGARCRDVAVIANPSHQEKGLTDTS 529
           +G+   G   GS L+L+KVFY+A + DW SA+ +PVGARCRDV V  N SHQ++GLT+ S
Sbjct: 421 EGQ---GDALGSPLSLAKVFYEANISDWFSAVAIPVGARCRDVGVATNSSHQKRGLTNYS 480

Query: 530 SFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISS-ERIQPSFDRIQHYFGTFGQLVCQLP 589
           SFGPPLL+ PNG A GL VRKSN  AS+A F+S    +QP    I   F TFGQ+VCQL 
Sbjct: 481 SFGPPLLNQPNGSAFGLMVRKSNFGASVAHFLSGLGNMQPGSVGITRCFSTFGQVVCQLS 540

Query: 590 RGTKLSVLGLLQATKLSNQHVNLGALTIPVCLSRHKSA--ETVEASDPRLLTVSGEAISR 649
           R TKLS+LG+ Q  KLS+Q VNLGAL +P+ + +H+ A   ++EAS   + +   E +  
Sbjct: 541 RSTKLSLLGIHQLPKLSSQQVNLGALALPIGIFKHRKAPETSMEASATPIESQLEENVMA 600

Query: 650 GSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNNNEDVLGWGMSLSGILGGPL 709
           GS+AL+LESELDE TRIGGW+E+ +S+PKYLQWAV+MSD   ED  GWG+SL G + GP 
Sbjct: 601 GSIALMLESELDESTRIGGWLEIKKSDPKYLQWAVTMSD-TPEDEFGWGLSLGGSIQGPK 660

Query: 710 DGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVRSNWSL 727
             D FQVE+++K N+ KRFSL+P + YV DG  +  A ++RS WSL
Sbjct: 661 SWDHFQVETFLKFNLGKRFSLQPALVYVMDGTTQFPALMLRSTWSL 702

BLAST of Lsi08G012970 vs. NCBI nr
Match: XP_038884063.1 (uncharacterized protein LOC120075002 [Benincasa hispida] >XP_038884064.1 uncharacterized protein LOC120075002 [Benincasa hispida] >XP_038884065.1 uncharacterized protein LOC120075002 [Benincasa hispida])

HSP 1 Score: 787.7 bits (2033), Expect = 8.4e-224
Identity = 395/424 (93.16%), Postives = 411/424 (96.93%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           MEP  FVVDKLKGFGTATQNFFDGLVHRREKSSRR+PIEILKRLQREAFSDLMRLRDRQD
Sbjct: 1   MEPVIFVVDKLKGFGTATQNFFDGLVHRREKSSRRNPIEILKRLQREAFSDLMRLRDRQD 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVEKVLSFYNTQRSSPFQE +THVKGEVNILGALLLMSV+DNHSFN LRRAGISTGI SR
Sbjct: 61  KVEKVLSFYNTQRSSPFQEISTHVKGEVNILGALLLMSVIDNHSFNTLRRAGISTGIQSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
           LTFETTVRE+DSLVAEFVANQK  +DFGVDSGSELTLSKV YKA V DWMSAIVVPVGAR
Sbjct: 121 LTFETTVRESDSLVAEFVANQKATMDFGVDSGSELTLSKVLYKANVGDWMSAIVVPVGAR 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
           CRDVAVIANPSHQEKGLTDTSSFGPPLLD P+GGA+GLTVRKSNVTASLAQFIS+ERIQP
Sbjct: 181 CRDVAVIANPSHQEKGLTDTSSFGPPLLDQPSGGAVGLTVRKSNVTASLAQFISTERIQP 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKLSNQHVNLGALTIPVCLSRHKSAET 602
           SFDRI+HYFGTFGQLVCQLPRGTKLSVLGLLQATKLSNQHVNLGALTIPVCLSRHKS+ET
Sbjct: 241 SFDRIRHYFGTFGQLVCQLPRGTKLSVLGLLQATKLSNQHVNLGALTIPVCLSRHKSSET 300

Query: 603 VEASDPRLLTVSGEAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNNN 662
           VEA DPRLLTVSGEAISRGS+ALLLESELDEVTRIGGWVELSQSNPKYLQWAVSM+DNNN
Sbjct: 301 VEAPDPRLLTVSGEAISRGSIALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMADNNN 360

Query: 663 EDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVRS 722
           ED LGWGMSLSGILGGPLDG+QFQ+ESYVKLNMSKRF+LKPGIAYVTDGNAKMMAF+VRS
Sbjct: 361 EDALGWGMSLSGILGGPLDGNQFQIESYVKLNMSKRFNLKPGIAYVTDGNAKMMAFLVRS 420

Query: 723 NWSL 727
           NWSL
Sbjct: 421 NWSL 424

BLAST of Lsi08G012970 vs. NCBI nr
Match: XP_008445322.1 (PREDICTED: uncharacterized protein LOC103488388 [Cucumis melo])

HSP 1 Score: 751.9 bits (1940), Expect = 5.1e-213
Identity = 383/425 (90.12%), Postives = 397/425 (93.41%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           MEP TFVVDKLKGFGTATQNFF GLVHRREKS+RRSPIEILKRLQREAFSDLMRLRDRQD
Sbjct: 1   MEPVTFVVDKLKGFGTATQNFFYGLVHRREKSARRSPIEILKRLQREAFSDLMRLRDRQD 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVEKVLS YNTQRSSPFQENATHVKGEVNILGALL MSV+DNHSF+AL RAGISTGIHSR
Sbjct: 61  KVEKVLSLYNTQRSSPFQENATHVKGEVNILGALLFMSVIDNHSFDALHRAGISTGIHSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
           LTFETTVRE+DSLVAEFVANQK KVDFGVDSGSELTLSKV YKA V DWMSA VVPVGAR
Sbjct: 121 LTFETTVRESDSLVAEFVANQKAKVDFGVDSGSELTLSKVLYKANVGDWMSATVVPVGAR 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
           CRDVAVIANPSHQEKGLTD SSFGPPL D PNGGAIGLTVRKSN+TASLAQFIS+ERIQP
Sbjct: 181 CRDVAVIANPSHQEKGLTDASSFGPPLFDQPNGGAIGLTVRKSNLTASLAQFISTERIQP 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKL-SNQHVNLGALTIPVCLSRHKSAE 602
           SFDRIQH+ GTFGQLVCQLPRGTKLS+LGLLQ  KL SNQHVNLGALTIPVCLSR KS E
Sbjct: 241 SFDRIQHHLGTFGQLVCQLPRGTKLSLLGLLQVPKLSSNQHVNLGALTIPVCLSRRKSPE 300

Query: 603 TVEASDPRLLTVSGEAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNN 662
           TVE  DPRLLTVSGEAISR S+ALLLESELDEVTRIGGWVELSQSN KYLQWAVSMSDNN
Sbjct: 301 TVEGPDPRLLTVSGEAISRRSIALLLESELDEVTRIGGWVELSQSNSKYLQWAVSMSDNN 360

Query: 663 NEDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVR 722
           +ED LGWGMSLSGILG  LD D FQVESYVKLN+SK+F+LKPGIAYVTDGNAKMMAF+VR
Sbjct: 361 SEDALGWGMSLSGILGSSLDRDHFQVESYVKLNVSKKFNLKPGIAYVTDGNAKMMAFLVR 420

Query: 723 SNWSL 727
           SNWSL
Sbjct: 421 SNWSL 425

BLAST of Lsi08G012970 vs. NCBI nr
Match: XP_031737106.1 (uncharacterized protein LOC101215602 [Cucumis sativus] >KGN62266.1 hypothetical protein Csa_018590 [Cucumis sativus])

HSP 1 Score: 733.8 bits (1893), Expect = 1.4e-207
Identity = 373/426 (87.56%), Postives = 393/426 (92.25%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           MEP TFVVDKLKGFGTATQNFFDGLVHRREKS+RRSPIEILKRLQREAFSDLMRLRDRQD
Sbjct: 1   MEPVTFVVDKLKGFGTATQNFFDGLVHRREKSARRSPIEILKRLQREAFSDLMRLRDRQD 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVEKVLS YNTQRSSPFQEN THVKGEVN+LGALL MSV+DNHSF AL RAGISTGIHSR
Sbjct: 61  KVEKVLSLYNTQRSSPFQENVTHVKGEVNVLGALLFMSVIDNHSFKALHRAGISTGIHSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
           LTFETTVRE+DSLVAEFVANQK KVDFGVDSGSELTLSKV YKA V DWMSA VVPVGAR
Sbjct: 121 LTFETTVRESDSLVAEFVANQKAKVDFGVDSGSELTLSKVLYKASVGDWMSATVVPVGAR 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
           CRDV++IANPSHQEKGLTD SSFGPPLLD PNGGAIGLTVRKSN+TASLAQFIS+ERIQP
Sbjct: 181 CRDVSIIANPSHQEKGLTDASSFGPPLLDQPNGGAIGLTVRKSNLTASLAQFISTERIQP 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKL-SNQHVNLGALTIPVCLSRHKSAE 602
           SFDRIQH+ GTFGQLVCQLPRG KLS+LGLLQ  KL SNQHVNLGALTIPVCLSR KS E
Sbjct: 241 SFDRIQHHLGTFGQLVCQLPRGMKLSLLGLLQVPKLSSNQHVNLGALTIPVCLSRRKSPE 300

Query: 603 TVEASDPRLLTVSG-EAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDN 662
           TV+  DPRLLTVSG EAISR S+ALLLESELDE TRI GWVELSQSN KYL+WAVS+SDN
Sbjct: 301 TVDGPDPRLLTVSGEEAISRRSIALLLESELDEETRIAGWVELSQSNSKYLRWAVSVSDN 360

Query: 663 NNEDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVV 722
           N+ED LGWGMSLSGILG  +D D FQVESYVKLN+SK+F+LKPGIAYVTDGNAKMMAF+V
Sbjct: 361 NSEDALGWGMSLSGILGSAIDRDHFQVESYVKLNVSKKFNLKPGIAYVTDGNAKMMAFLV 420

Query: 723 RSNWSL 727
           RS WSL
Sbjct: 421 RSKWSL 426

BLAST of Lsi08G012970 vs. NCBI nr
Match: XP_022144126.1 (uncharacterized protein LOC111013892 [Momordica charantia])

HSP 1 Score: 696.0 bits (1795), Expect = 3.3e-196
Identity = 360/425 (84.71%), Postives = 386/425 (90.82%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           MEP T+VVDKLKGFGTA+ NF DGLVHRREKSSRR PIEILKRLQREAFSDLMRLRDRQD
Sbjct: 1   MEPITYVVDKLKGFGTASHNFIDGLVHRREKSSRRRPIEILKRLQREAFSDLMRLRDRQD 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVEK LSFY TQ+SSPFQE+ATHVKGEV++LGALLLMSV+D +S NAL RAGISTGIHSR
Sbjct: 61  KVEKTLSFYGTQKSSPFQESATHVKGEVDLLGALLLMSVIDRNSCNALCRAGISTGIHSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
           LTFE TVRENDSLVAEFVANQK  VDFGV SGSELTLSKVFYKA V DWMSAI +P+GAR
Sbjct: 121 LTFEATVRENDSLVAEFVANQKAIVDFGVHSGSELTLSKVFYKASVGDWMSAIAIPLGAR 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
           CRDVAVIANPSHQEKGLTD SSFGPPLLD P+GGAIGLTVRKSNVTASLAQFIS+ERIQP
Sbjct: 181 CRDVAVIANPSHQEKGLTDISSFGPPLLDQPSGGAIGLTVRKSNVTASLAQFISTERIQP 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKLSNQHVNLGALTIPVCL-SRHKSAE 602
           SFDRIQHY  TFGQLV QLPRGTKLS+LGLLQA K + QHVNLGALT+PV L SRH S E
Sbjct: 241 SFDRIQHYLSTFGQLVYQLPRGTKLSLLGLLQAPKFT-QHVNLGALTLPVSLGSRHTSPE 300

Query: 603 TVEASDPRLLTVSGEAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNN 662
           TVEA DP   TVS +A+SRGS+ALLLESELDE+TRIGGWVE+SQSNPK++QWAVS+SD N
Sbjct: 301 TVEAPDPLSSTVSRKAMSRGSIALLLESELDELTRIGGWVEVSQSNPKHMQWAVSVSD-N 360

Query: 663 NEDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVR 722
           +ED LGWGMSLSGI  GPLD  QFQVESYVKLNMSKRFSLKPGIAYVTDG+AKMMAF+VR
Sbjct: 361 SEDALGWGMSLSGITQGPLDRSQFQVESYVKLNMSKRFSLKPGIAYVTDGDAKMMAFLVR 420

Query: 723 SNWSL 727
           SNWSL
Sbjct: 421 SNWSL 423

BLAST of Lsi08G012970 vs. TAIR 10
Match: AT1G11320.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion, plastid; EXPRESSED IN: 7 plant structures; EXPRESSED DURING: C globular stage, F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage, D bilateral stage; Has 46 Blast hits to 46 proteins in 14 species: Archae - 0; Bacteria - 2; Metazoa - 0; Fungi - 0; Plants - 44; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 376.7 bits (966), Expect = 4.2e-104
Identity = 193/425 (45.41%), Postives = 274/425 (64.47%), Query Frame = 0

Query: 303 MEPATFVVDKLKGFGTATQNFFDGLVHRREKSSRRSPIEILKRLQREAFSDLMRLRDRQD 362
           M+P   V++K+K F  ++Q+         E  SR +PI+ILKRLQRE FSDLM+LR+RQ+
Sbjct: 1   MDPIASVLEKVKSFTKSSQDLVSRHFGFHENPSRLNPIDILKRLQRETFSDLMKLRERQE 60

Query: 363 KVEKVLSFYNTQRSSPFQENATHVKGEVNILGALLLMSVVDNHSFNALRRAGISTGIHSR 422
           KVE+++S     +  PFQE +THV+GEV++LGA+LLM   D  SFN L + G+  G+ SR
Sbjct: 61  KVERIISSQKLSKGGPFQETSTHVRGEVDVLGAILLMGNTDEESFNGLNKEGVRPGLLSR 120

Query: 423 LTFETTVRENDSLVAEFVANQKGKVDFGVDSGSELTLSKVFYKARVRDWMSAIVVPVGAR 482
             FET++RE D LVAE VA   G        G EL+L+KVFYKA + DW SAI +PVGA 
Sbjct: 121 FVFETSLRETDRLVAELVAGYNGAGSHRDFLGGELSLAKVFYKADINDWFSAIAIPVGAH 180

Query: 483 CRDVAVIANPSHQEKGLTDTSSFGPPLLDHPNGGAIGLTVRKSNVTASLAQFISSERIQP 542
            RD+      S+Q   LT+ S  GPPLL   NG AIGLT RKSN+TASLAQ +S+  ++ 
Sbjct: 181 FRDIEASTVSSYQGMSLTEVSELGPPLLSQHNGSAIGLTFRKSNITASLAQSMSNVEVEQ 240

Query: 543 SFDRIQHYFGTFGQLVCQLPRGTKLSVLGLLQATKLSNQHVNLGALTIPVC-LSRHKSAE 602
                   F TFGQ+ C + R  KLS+LG  Q    S+   ++GA+T+PV  L RH + E
Sbjct: 241 GLTAPNRCFRTFGQVTCHILRSLKLSLLGCHQVLTSSSNLHSVGAITVPVSFLRRHTATE 300

Query: 603 TVEASDPRLLTVSGEAISRGSLALLLESELDEVTRIGGWVELSQSNPKYLQWAVSMSDNN 662
              ++ P  ++ +   +S  S+AL L+S +DE T++GGW+E+  S  K ++W+VS++D N
Sbjct: 301 PEPSAPPLEMSRNMNHVSSSSIALKLDSLMDESTKLGGWIEIQNSREKQVKWSVSITD-N 360

Query: 663 NEDVLGWGMSLSGILGGPLDGDQFQVESYVKLNMSKRFSLKPGIAYVTDGNAKMMAFVVR 722
            ED +GWGMS+ G++ G  + DQFQVESY+K N+  RF L P + Y T+ + + +  +++
Sbjct: 361 PEDEVGWGMSVGGVVDGSRNHDQFQVESYLKFNIGDRFRLSPCLVYHTNSDGRTIGLMLK 420

Query: 723 SNWSL 727
           S+WSL
Sbjct: 421 SHWSL 424

BLAST of Lsi08G012970 vs. TAIR 10
Match: AT1G75880.2 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.6e-84
Identity = 156/290 (53.79%), Postives = 204/290 (70.34%), Query Frame = 0

Query: 15  SFKLNVFQCLQLLLILQPAS---AAIKIPPGYSVPAVFVFGDSIVDTGNNNDLITQAKCN 74
           S  L +F C+ +L++L   S   A +KIP   +VPAV VFGDSIVD GNN+D+IT+A+C+
Sbjct: 17  SSSLILFWCIFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMITEARCD 76

Query: 75  YPPYGRDFPGGRPTGRFSNGRVPSDLV----GIKAFLPPYVNTNLQPEDLITGVNFASGG 134
           Y PYG DF GG  TGRFSNG+VP D+V    GIK  +P Y N NL+PE+L+TGV FASGG
Sbjct: 77  YAPYGIDFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGG 136

Query: 135 AGFDPLTSKTAPAISLEAQLAMFREYKKKLEGLVGEERAKFIIDNSLYLVVAGSNDIANT 194
           AG+ PLT+K A  I L  QL  F EY +KL+ +VGE+R KFII NSL++V+ GSNDIAN 
Sbjct: 137 AGYVPLTTKIAGGIPLPQQLIYFEEYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIAND 196

Query: 195 FYLARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRRIGFFGTPPLGCVPSQRTLAGGI 254
           F+     ++HY + ++T  M  +A +F + LY  G RRI  FG PP+GCVPSQRT+AGG 
Sbjct: 197 FFTLPPVRLHYTVASFTALMADNARSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGGP 256

Query: 255 ERACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYVDIYNPLLDVIQN 298
            R CV  +N A++LFN KL + +D L   L D  ++Y+DIY+PLLD+I N
Sbjct: 257 TRDCVARFNDAAKLFNTKLSANIDVLSRTLQDPTIIYIDIYSPLLDLILN 306

BLAST of Lsi08G012970 vs. TAIR 10
Match: AT1G75880.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 307.0 bits (785), Expect = 4.1e-83
Identity = 156/291 (53.61%), Postives = 204/291 (70.10%), Query Frame = 0

Query: 15  SFKLNVFQCLQLLLILQPAS---AAIKIPPGYSVPAVFVFGDSIVDTGNNNDLITQAKCN 74
           S  L +F C+ +L++L   S   A +KIP   +VPAV VFGDSIVD GNN+D+IT+A+C+
Sbjct: 17  SSSLILFWCIFVLVLLSTTSTTNALVKIPKNTTVPAVIVFGDSIVDAGNNDDMITEARCD 76

Query: 75  YPPYGRDFPGGRPTGRFSNGRVPSDLV----GIKAFLPPYVNTNLQPEDLITGVNFASGG 134
           Y PYG DF GG  TGRFSNG+VP D+V    GIK  +P Y N NL+PE+L+TGV FASGG
Sbjct: 77  YAPYGIDFDGGVATGRFSNGKVPGDIVAEELGIKPNIPAYRNPNLKPEELLTGVTFASGG 136

Query: 135 AGFDPLTSKTA-PAISLEAQLAMFREYKKKLEGLVGEERAKFIIDNSLYLVVAGSNDIAN 194
           AG+ PLT+K A   I L  QL  F EY +KL+ +VGE+R KFII NSL++V+ GSNDIAN
Sbjct: 137 AGYVPLTTKIAVGGIPLPQQLIYFEEYIEKLKQMVGEKRTKFIIKNSLFVVICGSNDIAN 196

Query: 195 TFYLARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRRIGFFGTPPLGCVPSQRTLAGG 254
            F+     ++HY + ++T  M  +A +F + LY  G RRI  FG PP+GCVPSQRT+AGG
Sbjct: 197 DFFTLPPVRLHYTVASFTALMADNARSFAQTLYGYGARRILVFGAPPIGCVPSQRTVAGG 256

Query: 255 IERACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYVDIYNPLLDVIQN 298
             R CV  +N A++LFN KL + +D L   L D  ++Y+DIY+PLLD+I N
Sbjct: 257 PTRDCVARFNDAAKLFNTKLSANIDVLSRTLQDPTIIYIDIYSPLLDLILN 307

BLAST of Lsi08G012970 vs. TAIR 10
Match: AT1G75900.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 302.8 bits (774), Expect = 7.7e-82
Identity = 151/288 (52.43%), Postives = 204/288 (70.83%), Query Frame = 0

Query: 14  SSFKLNVFQCLQLLLILQPASAAIKIPPGYSVPAVFVFGDSIVDTGNNNDLITQAKCNYP 73
           SS+K+    CL  +L L     A+K+PP   +PAV  FGDSIVDTG NN++ T  KC++ 
Sbjct: 14  SSWKI----CLLSVLFLTETITAVKLPPKLIIPAVIAFGDSIVDTGMNNNVKTVVKCDFL 73

Query: 74  PYGRDFPGGRPTGRFSNGRVPSDL----VGIKAFLPPYVNTNLQPEDLITGVNFASGGAG 133
           PYG +F  G  TGRF +GRVP+DL    +GIK+ +P Y++ NL+ +DL+TGV+FASGG+G
Sbjct: 74  PYGINFQSGVATGRFCDGRVPADLLAEELGIKSIVPAYLDPNLKSKDLLTGVSFASGGSG 133

Query: 134 FDPLTSKTAPAISLEAQLAMFREYKKKLEGLVGEERAKFIIDNSLYLVVAGSNDIANTFY 193
           +DP+T K    ISLE QL+ F EY +K++ +VGE R  FI+ NSL+L+VAGS+DIANT+Y
Sbjct: 134 YDPITPKLVAVISLEDQLSYFEEYIEKVKNIVGEARKDFIVANSLFLLVAGSDDIANTYY 193

Query: 194 LARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRRIGFFGTPPLGCVPSQRTLAGGIER 253
             R R   Y++++YT  M  SAS FV +LY  G RR+  FG PP+GCVPSQRTL GGI R
Sbjct: 194 TLRARP-EYDVDSYTTLMSDSASEFVTKLYGYGVRRVAVFGAPPIGCVPSQRTLGGGILR 253

Query: 254 ACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYVDIYNPLLDVIQN 298
            C + YN+A++LFN KL   LD L+  LP  + +Y++IY+PL D+IQN
Sbjct: 254 DCADNYNEAAKLFNSKLSPKLDSLRKTLPGIKPIYINIYDPLFDIIQN 296

BLAST of Lsi08G012970 vs. TAIR 10
Match: AT1G75890.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 6.1e-79
Identity = 147/289 (50.87%), Postives = 199/289 (68.86%), Query Frame = 0

Query: 14  SSFKLNVFQCLQLLLILQPAS--AAIKIPPGYSVPAVFVFGDSIVDTGNNNDLITQ-AKC 73
           +SF  + F C+  L++L   S  A +K PP  + PA+ VFGDSIVD GNN+D++T  A+C
Sbjct: 12  TSFSSSPFWCVFFLVLLCKTSTNALVKQPPNETTPAIIVFGDSIVDAGNNDDIMTTLARC 71

Query: 74  NYPPYGRDFPGGRPTGRFSNGRVPSDLV----GIKAFLPPYVNTNLQPEDLITGVNFASG 133
           NYPPYG DF GG PTGRF NG+V +D +    GIK  +P Y N NL+PEDL+TGV FASG
Sbjct: 72  NYPPYGIDFDGGIPTGRFCNGKVATDFIAGKFGIKPSIPAYRNPNLKPEDLLTGVTFASG 131

Query: 134 GAGFDPLTSKTAPAISLEAQLAMFREYKKKLEGLVGEERAKFIIDNSLYLVVAGSNDIAN 193
           GAG+ P T++ +  I+L  QL +F EY +K++ +VGEER K II NSL++V+ GSNDI N
Sbjct: 132 GAGYVPFTTQLSGGIALSQQLKLFEEYVEKMKKMVGEERTKLIIKNSLFMVICGSNDITN 191

Query: 194 TFYLARIRQVHYNINTYTDFMVQSASTFVKELYAAGGRRIGFFGTPPLGCVPSQRTLAGG 253
           T++     Q  Y++ ++T  M  +A +F ++L+  G RRI  FG PP+GCVPSQRTLAGG
Sbjct: 192 TYFGLPSVQQQYDVASFTTLMADNARSFAQKLHEYGARRIQVFGAPPVGCVPSQRTLAGG 251

Query: 254 IERACVNEYNKASELFNGKLHSTLDYLQTILPDSRVVYVDIYNPLLDVI 296
             R CV  +N A++L+N KL + L  L   L D  ++YVDIY+ LLD+I
Sbjct: 252 PTRNCVVRFNDATKLYNVKLAANLGSLSRTLGDKTIIYVDIYDSLLDII 300