Lsi08G012510 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G012510
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	early nodulin-like protein 1
Location	chr08: 20976998 .. 20979817 (+)
RNA-Seq Expression	Lsi08G012510
Synteny	Lsi08G012510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTAAACGACGACGCATCGAGGAAGACAACGGGGTCGGTATTAAGCCAAAAGAGAAACCTTCCTTTGAGCAACACCGGCATGGCTATTGCTGCCACTCTCCTCCTCGGAATCGGCTACATGGTTTATGTCAGCAGATCCAGAAATGTTAGTAATAATAATATTAATCGCCAATCTAGTCGCTGATTTTTTTTTATATTTTATTTTTCAAGGAATATGTTTATTTTTATTTTTTTTGTTTTTTCTGTTTATTTATTGCATGTGTGTGTGTGTATTTGGTCTTCCTTTTATACTGTAAAAGCATATAAATAGAGATCATCTAAGCCTTTTCTTTAATTATTGGGTCAAATTTTGTCAGTGACTTCAAGTTGTATGGGTATATTTGCTAAATGGTAATATTTTTTGGACTTTTTCTTTTTCCTGGGTATAGTTTCCCTTTAATTTATTTTTTAATTTCTGATTTTTAGAATCTTGTTTATTTCCACACAAATCTGAGTGTAAATTCTAAAAATATCATCTTATATCATCTTATATTTTTCAATGTATCGAGATTTTAAATTTGAAAATTTAAAATTGCATATCCTTTGTTTCTAATGTATCTAAATTCACTAAATTTTGTATTTGAAAAATTAAAATGCACAATTATATTAGAGAAGATAAAATTATTTCAGATTATAAACTGAATTTTTTTTTTGTTAAATTAAAATATGTATTATATAATATTTAATATATTATATGTACTACAAAAGAATAATTACACAAATTTTAACATAATAAATTAATAAAAATTTATATTTAATTTAATATTTAGTGAGTAATTATAATTATATATTATAAATATAATTTTTTAAAATATTCTTTTAGCGGTTTTAAAAATTGGGCGTTTTCAAATATGGAAAGTCTATCCATCAAGCCATAGAATTCATAAATAGTTTAATTTTTTAATTTTTTCTTAATGAAGGAAAACCAACATAAAAAATATTTATATTCCATCTTTTATTGTTGTTGTGAAACTTGCAATATATGCATTTGAATATTTAATCATAAATCTAAATATCAAGTTTCAATTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAGGAAGATAAAATTTCGTTGGATAATGTAGAAAAGAAAAGGTGGTGATCAAACAACGAGCAAATGATAATTCATTTGAAAATGACTGGTTGCTTTTTTCTAAATAAAATTGAAATATGTCTTGAGGAAAAAACCAAGAACAAAACCGAGATTATAGAATTACACTAAGGAAAACAAAAGGATCCAAATTCATGGTAAAGGGATGAGAGAAGAGAAGAAGCAACCAAATCAGTGTTCAACTTTCAATGGTGTCTTAGTGTCATCGCAAATATTACAACCATATTTTTTAAAAATGGCTATTTCGATTATTATTATTTTTAGTTTAAAGAGATCAGAGTGATCATATTTTCTATCAAAAGAAAAAAAAGTAATTAAAAAGTAAAAAATATCAAAATGAACCATTCTTGAACTAAAAGAACTCAAAGGTGAAAAGTATAGAGATTAGAATAAATATTTTAATTAAAAACAAAAGATCTAAAATGAATGAAAGTAAAATATATATATAGAGAGAGACTTAAACAAAAAATTAAAAAAGTAATAACCTCATCCTTTCAAAATAGGAATAATAAATGTTTTACATAATAATAATAATAATATCCTCTGCAAAGTGCCAGCCAAAATATAACCGTTGGATTATAAGCCGTTTAAAAGTTCCCTTTAGCCATTCTCCACGCATCTTAGCCGTTGGAACTTTGAAAGGGAAGGAAATAAAAGACAAAAACCCAACATACACTCAGGAGTCAGATGGCCGATTAAAGTTAAAAACCCCAACGGCTAGTTTTCCTCTTTCTTCCACCTCTGCATATAAAATGGAAAGCCCCTTCTCCTCAAAACTCATTCTTTCATTCATTTTTTTTCCAATTAGTCATTTTTTTTCTCATCTCTGTTTCTCACTCCATCGGTGAAGAAGAAGAAAGATCCCATTCCCATTCATCGTCATCATCCATCCATGGCTGGTTTTTCAAGGGCTTTGTTTCTTCTCACATTTTTCCTCTTCAGCTTCTCCGAAGCTAGAGAGATCTTGGTCGGGGGCAAATCCAATGCCTGGAAAATCCCTTCTTCTCAATCTCAATCTCTCAATCAATGGGCTGAAACCTCCCGCTTTCGCGTCGGCGATACTCTCGGTAACTTCCTCTACTCTGTTTTTCACGACCTCTGTTTTTTGTGGGAATTGTTGAATGATCTAACTTGATTTTTTGCTTGATTGAATGTTAACAGTGTGGAATTACGAAGAAGGGAAAGATTCGGTACTGAAAGTGAGGAAGGAAGATTATGAAGCCTGCAATACCGAAAACCCAGAACAGATATTTGAGGATGGGAATACGAAAGTGGAGCTACACAAACCAGGCCCATTTTATTTCATCAGTGGGGCAAATGGTCACTGCGAGCAGGGCCAGAAGCTGATTGTTGTGGTTGTGACTCCTCGCCGAAGGTTCATAGGCATCTCTCCGGCGCCTTCTCCGGCGGAGAGTGAGGGTCCAGCAGTTGCTCCGTCGAGTGGAGCTGCAAATTTGAAGGTTGGGTTTTTGGCGGTAATTGTCGGAATTTTGGGTGTTGGAGTTGGATTGCTGTGA

mRNA sequence

ATGGCATTAAACGACGACGCATCGAGGAAGACAACGGGGTCGGTATTAAGCCAAAAGAGAAACCTTCCTTTGAGCAACACCGGCATGGCTATTGCTGCCACTCTCCTCCTCGGAATCGGCTACATGGTTTATAAGAAGAAAGATCCCATTCCCATTCATCGTCATCATCCATCCATGGCTGGTTTTTCAAGGGCTTTGTTTCTTCTCACATTTTTCCTCTTCAGCTTCTCCGAAGCTAGAGAGATCTTGGTCGGGGGCAAATCCAATGCCTGGAAAATCCCTTCTTCTCAATCTCAATCTCTCAATCAATGGGCTGAAACCTCCCGCTTTCGCGTCGGCGATACTCTCGTGTGGAATTACGAAGAAGGGAAAGATTCGGTACTGAAAGTGAGGAAGGAAGATTATGAAGCCTGCAATACCGAAAACCCAGAACAGATATTTGAGGATGGGAATACGAAAGTGGAGCTACACAAACCAGGCCCATTTTATTTCATCAGTGGGGCAAATGGTCACTGCGAGCAGGGCCAGAAGCTGATTGTTGTGGTTGTGACTCCTCGCCGAAGGTTCATAGGCATCTCTCCGGCGCCTTCTCCGGCGGAGAGTGAGGGTCCAGCAGTTGCTCCGTCGAGTGGAGCTGCAAATTTGAAGGTTGGGTTTTTGGCGGTAATTGTCGGAATTTTGGGTGTTGGAGTTGGATTGCTGTGA

Coding sequence (CDS)

ATGGCATTAAACGACGACGCATCGAGGAAGACAACGGGGTCGGTATTAAGCCAAAAGAGAAACCTTCCTTTGAGCAACACCGGCATGGCTATTGCTGCCACTCTCCTCCTCGGAATCGGCTACATGGTTTATAAGAAGAAAGATCCCATTCCCATTCATCGTCATCATCCATCCATGGCTGGTTTTTCAAGGGCTTTGTTTCTTCTCACATTTTTCCTCTTCAGCTTCTCCGAAGCTAGAGAGATCTTGGTCGGGGGCAAATCCAATGCCTGGAAAATCCCTTCTTCTCAATCTCAATCTCTCAATCAATGGGCTGAAACCTCCCGCTTTCGCGTCGGCGATACTCTCGTGTGGAATTACGAAGAAGGGAAAGATTCGGTACTGAAAGTGAGGAAGGAAGATTATGAAGCCTGCAATACCGAAAACCCAGAACAGATATTTGAGGATGGGAATACGAAAGTGGAGCTACACAAACCAGGCCCATTTTATTTCATCAGTGGGGCAAATGGTCACTGCGAGCAGGGCCAGAAGCTGATTGTTGTGGTTGTGACTCCTCGCCGAAGGTTCATAGGCATCTCTCCGGCGCCTTCTCCGGCGGAGAGTGAGGGTCCAGCAGTTGCTCCGTCGAGTGGAGCTGCAAATTTGAAGGTTGGGTTTTTGGCGGTAATTGTCGGAATTTTGGGTGTTGGAGTTGGATTGCTGTGA

Protein sequence

MALNDDASRKTTGSVLSQKRNLPLSNTGMAIAATLLLGIGYMVYKKKDPIPIHRHHPSMAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL

Homology

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BLAST of Lsi08G012510 vs. ExPASy Swiss-Prot
Match: Q9SK27 (Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 SV=2)

HSP 1 Score: 176.8 bits (447), Expect = 2.9e-43
Identity = 91/164 (55.49%), Postives = 120/164 (73.17%), Query Frame = 0


Query: 65  ALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGK 124
           A+F L  FLFS + A E+ VGGKS  WKIP S S S  +WA+ +RF+VGD +V+ YE GK
Sbjct: 15  AIFSL-IFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYESGK 74

Query: 125 DSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVT 184
           DSVL+V KE Y +CNT NP   + DG TKV+L + GPFYFISGANGHCE+GQKL +VV++
Sbjct: 75  DSVLEVTKEAYNSCNTTNPLANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLVVIS 134

Query: 185 PRRRFIGISPAPSPAESE-GPAVAPSSGAANLKVGFLAVIVGIL 228
           PR     ISPAPSP E E GPA+AP+  + ++++G   V++G++
Sbjct: 135 PRHSV--ISPAPSPVEFEDGPALAPAPISGSVRLGGCYVVLGLV 175

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BLAST of Lsi08G012510 vs. ExPASy Swiss-Prot
Match: Q8LC95 (Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 4.8e-38
Identity = 89/175 (50.86%), Postives = 120/175 (68.57%), Query Frame = 0


Query: 67  FLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGKDS 126
           FL+ F L +   ++EI+VGGK+++WKIPSS S+SLN+WAE+ RFRVGDTLVW Y+E KDS
Sbjct: 11  FLIFFLLTNLVCSKEIIVGGKTSSWKIPSSPSESLNKWAESLRFRVGDTLVWKYDEEKDS 70

Query: 127 VLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVTPR 186
           VL+V K+ Y  CNT NP   + +G+TKV+L + GP++FISG+  +C +G+KL +VV++ R
Sbjct: 71  VLQVTKDAYINCNTTNPAANYSNGDTKVKLERSGPYFFISGSKSNCVEGEKLHIVVMSSR 130

Query: 187 RRFIG-------ISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
               G        SPAPSPA    P VAP+SG +      L   VG+LG  VGLL
Sbjct: 131 GGHTGGFFTGSSPSPAPSPALLGAPTVAPASGGS---ASSLTRQVGVLGF-VGLL 181

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BLAST of Lsi08G012510 vs. ExPASy Swiss-Prot
Match: Q02917 (Early nodulin-55-2 OS=Glycine max OX=3847 GN=ENOD55-2 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.6e-25
Identity = 68/172 (39.53%), Postives = 99/172 (57.56%), Query Frame = 0


Query: 66  LFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGKD 125
           + L    L S SEA + +VGG   +WK P S+  SL+ WA + RF++GDTL++ YE+  +
Sbjct: 13  VMLAMCLLISTSEAEKYVVGGSEKSWKFPLSKPDSLSHWANSHRFKIGDTLIFKYEKRTE 72

Query: 126 SVLKVRKEDYEACNTENPEQI-FEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVT 185
           SV +  + DYE CNT     I F  GNTKV L KPG  +FISG   HC+ G KL V+V++
Sbjct: 73  SVHEGNETDYEGCNTVGKYHIVFNGGNTKVMLTKPGFRHFISGNQSHCQMGLKLAVLVIS 132

Query: 186 ---PRRRFIGISPAPSPAESEGPAVAPSSGAANLKV------GFLAVIVGIL 228
               ++  +  SP+PSP  S   + +PS    N  V      GF+ V++ ++
Sbjct: 133 SNKTKKNLLSPSPSPSPPPSSLLSPSPSPLPNNQGVTSSSGAGFIGVMMWLM 184

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BLAST of Lsi08G012510 vs. ExPASy Swiss-Prot
Match: Q9T076 (Early nodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g27520 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.9e-24
Identity = 63/145 (43.45%), Postives = 93/145 (64.14%), Query Frame = 0


Query: 73  LFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGKDSVLKVRK 132
           LF+ S AR+  VGG S AW   ++  ++   W+  +RF V DTL ++Y +G DSVL+V K
Sbjct: 22  LFTISNARKFNVGG-SGAW--VTNPPENYESWSGKNRFLVHDTLYFSYAKGADSVLEVNK 81

Query: 133 EDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVTPRRRFIGI 192
            DY+ACNT+NP +  +DG++++ L + GPFYFISG   +C++GQKL VVV++ R      
Sbjct: 82  ADYDACNTKNPIKRVDDGDSEISLDRYGPFYFISGNEDNCKKGQKLNVVVISARIPSTAQ 141

Query: 193 SP-APSPAESEGPAVAPSSGAANLK 217
           SP A +P  S   ++ P  GA + K
Sbjct: 142 SPHAAAPGSSTPGSMTPPGGAHSPK 163

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BLAST of Lsi08G012510 vs. ExPASy Swiss-Prot
Match: P93329 (Early nodulin-20 OS=Medicago truncatula OX=3880 GN=ENOD20 PE=3 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.9e-21
Identity = 64/178 (35.96%), Postives = 86/178 (48.31%), Query Frame = 0


Query: 59  MAGFSRALFLLTF---FLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDT 118
           M+  S  L +  F    L S+SE+ + LVG   N+WK P     +L +WA   +F VGDT
Sbjct: 1   MSSSSPILLMFIFSIWMLISYSESTDYLVGDSENSWKFPLPTRHALTRWASNYQFIVGDT 60

Query: 119 LVWNYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQG 178
           + + Y    +SV +V +EDY+ C          DGNT V L K G  +FISG   HC  G
Sbjct: 61  ITFQYNNKTESVHEVEEEDYDRCGIRGEHVDHYDGNTMVVLKKTGIHHFISGKKRHCRLG 120

Query: 179 QKLIVVVV---------------TPR---------RRFIGISPAPSPAESEGPAVAPS 210
            KL VVV+               TPR         RR +   P+PSP+ S  P+ +PS
Sbjct: 121 LKLAVVVMVAPVLSSPPPPPSPPTPRSSTPIPHPPRRSLPSPPSPSPSPSPSPSPSPS 178

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BLAST of Lsi08G012510 vs. ExPASy TrEMBL
Match: A0A5A7VC90 (Early nodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001970 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.7e-84
Identity = 162/176 (92.05%), Postives = 166/176 (94.32%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MA FS ALFL TF LFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFR+GDTLVW
Sbjct: 1   MANFSIALFLFTFLLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRIGDTLVW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
            YEEGKDSVLKV KEDYEACNTENPEQ FEDG TKVEL KPGPFYFISGA GHCEQGQKL
Sbjct: 61  TYEEGKDSVLKVTKEDYEACNTENPEQRFEDGKTKVELEKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           IVVV+TPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAV+VGILGVGVGL+
Sbjct: 121 IVVVLTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVVVGILGVGVGLV 176

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BLAST of Lsi08G012510 vs. ExPASy TrEMBL
Match: A0A1S3BBT8 (early nodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103488354 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 2.7e-84
Identity = 162/176 (92.05%), Postives = 166/176 (94.32%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MA FS ALFL TF LFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFR+GDTLVW
Sbjct: 1   MANFSIALFLFTFLLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRIGDTLVW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
            YEEGKDSVLKV KEDYEACNTENPEQ FEDG TKVEL KPGPFYFISGA GHCEQGQKL
Sbjct: 61  TYEEGKDSVLKVTKEDYEACNTENPEQRFEDGKTKVELEKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           IVVV+TPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAV+VGILGVGVGL+
Sbjct: 121 IVVVLTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVVVGILGVGVGLV 176

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BLAST of Lsi08G012510 vs. ExPASy TrEMBL
Match: A0A0A0LQG0 (Phytocyanin-like arabinogalactan-protein OS=Cucumis sativus OX=3659 GN=Csa_2G382700 PE=4 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 4.0e-80
Identity = 158/178 (88.76%), Postives = 163/178 (91.57%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFL--FSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTL 118
           MA FS AL L TF L   SFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFR+GDTL
Sbjct: 1   MANFSIALSLFTFLLLFISFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRIGDTL 60

Query: 119 VWNYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQ 178
           VW+YE+GKDSVLKV KEDYEACNTENPEQ FEDG TKVEL KPGPFYFISGA GHCEQGQ
Sbjct: 61  VWDYEDGKDSVLKVTKEDYEACNTENPEQRFEDGKTKVELEKPGPFYFISGAKGHCEQGQ 120

Query: 179 KLIVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           KLIVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGA NLKVG LAV+VGILGVGVGL+
Sbjct: 121 KLIVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAGNLKVGLLAVVVGILGVGVGLV 178

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BLAST of Lsi08G012510 vs. ExPASy TrEMBL
Match: A0A6J1K2Z5 (early nodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111491916 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 6.8e-80
Identity = 151/176 (85.80%), Postives = 162/176 (92.05%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MAG SRALFLLTF L SFSEA EILVGGK+NAWK+PSSQSQSLNQWAETSRFRVGDTLVW
Sbjct: 1   MAGSSRALFLLTFLLLSFSEATEILVGGKANAWKLPSSQSQSLNQWAETSRFRVGDTLVW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
           +YEEGKDSVLKV KEDYEACNT+NPEQ FEDGNTKV+LHKPGPFYFISGA GHCEQGQKL
Sbjct: 61  SYEEGKDSVLKVGKEDYEACNTQNPEQRFEDGNTKVQLHKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           +VVVVTPRRRF+  SPAPSPAE EGPAV+PSSGAA+  VGFLA +VGILG+GVG L
Sbjct: 121 VVVVVTPRRRFVSFSPAPSPAEIEGPAVSPSSGAASFNVGFLAAVVGILGLGVGFL 176

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BLAST of Lsi08G012510 vs. ExPASy TrEMBL
Match: A0A6J1HCD3 (early nodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111462709 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.2e-79
Identity = 152/176 (86.36%), Postives = 159/176 (90.34%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MAG SRALFLLTF LFSFSEA EILVGGK NAWK+PSSQSQSLNQWAETSRFRVGDTL W
Sbjct: 1   MAGSSRALFLLTFLLFSFSEATEILVGGKPNAWKLPSSQSQSLNQWAETSRFRVGDTLAW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
           +YEEGKDSVLKV KEDYEACNT+NPEQ FEDGNTKVELHKPGPFYFISGA GHCEQGQKL
Sbjct: 61  SYEEGKDSVLKVGKEDYEACNTQNPEQRFEDGNTKVELHKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           +VVVVTPRRRF+  SPAPSP E EGPAVAPSSGAA+  VGFLA  VGILG+GVG L
Sbjct: 121 VVVVVTPRRRFVSFSPAPSPTEIEGPAVAPSSGAASFNVGFLAAAVGILGLGVGFL 176

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BLAST of Lsi08G012510 vs. NCBI nr
Match: XP_038884215.1 (early nodulin-like protein 1 [Benincasa hispida])

HSP 1 Score: 321.6 bits (823), Expect = 5.5e-84
Identity = 159/171 (92.98%), Postives = 164/171 (95.91%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MAGFSRALFLLTF LFSFSEAREILVGGKSN W IPSSQSQSLNQWAETSRFR+GDTLVW
Sbjct: 1   MAGFSRALFLLTFLLFSFSEAREILVGGKSNVWTIPSSQSQSLNQWAETSRFRIGDTLVW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
           NYEEGKDSVLKV KEDYEACNTENPEQ FEDGNTKVEL KPGPFYFISGA GHCEQGQKL
Sbjct: 61  NYEEGKDSVLKVTKEDYEACNTENPEQRFEDGNTKVELQKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGV 230
           IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAV+VG++G+
Sbjct: 121 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVVVGVMGL 171

[Close]

BLAST of Lsi08G012510 vs. NCBI nr
Match: XP_008445271.1 (PREDICTED: early nodulin-like protein 1 [Cucumis melo] >KAA0064884.1 early nodulin-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 321.6 bits (823), Expect = 5.5e-84
Identity = 162/176 (92.05%), Postives = 166/176 (94.32%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MA FS ALFL TF LFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFR+GDTLVW
Sbjct: 1   MANFSIALFLFTFLLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRIGDTLVW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
            YEEGKDSVLKV KEDYEACNTENPEQ FEDG TKVEL KPGPFYFISGA GHCEQGQKL
Sbjct: 61  TYEEGKDSVLKVTKEDYEACNTENPEQRFEDGKTKVELEKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           IVVV+TPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAV+VGILGVGVGL+
Sbjct: 121 IVVVLTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVVVGILGVGVGLV 176

[Close]

BLAST of Lsi08G012510 vs. NCBI nr
Match: XP_004138700.1 (early nodulin-like protein 1 [Cucumis sativus] >KGN62997.1 hypothetical protein Csa_022425 [Cucumis sativus])

HSP 1 Score: 307.8 bits (787), Expect = 8.2e-80
Identity = 158/178 (88.76%), Postives = 163/178 (91.57%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFL--FSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTL 118
           MA FS AL L TF L   SFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFR+GDTL
Sbjct: 1   MANFSIALSLFTFLLLFISFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRIGDTL 60

Query: 119 VWNYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQ 178
           VW+YE+GKDSVLKV KEDYEACNTENPEQ FEDG TKVEL KPGPFYFISGA GHCEQGQ
Sbjct: 61  VWDYEDGKDSVLKVTKEDYEACNTENPEQRFEDGKTKVELEKPGPFYFISGAKGHCEQGQ 120

Query: 179 KLIVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           KLIVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGA NLKVG LAV+VGILGVGVGL+
Sbjct: 121 KLIVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAGNLKVGLLAVVVGILGVGVGLV 178

[Close]

BLAST of Lsi08G012510 vs. NCBI nr
Match: XP_022996782.1 (early nodulin-like protein 1 [Cucurbita maxima])

HSP 1 Score: 307.0 bits (785), Expect = 1.4e-79
Identity = 151/176 (85.80%), Postives = 162/176 (92.05%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MAG SRALFLLTF L SFSEA EILVGGK+NAWK+PSSQSQSLNQWAETSRFRVGDTLVW
Sbjct: 1   MAGSSRALFLLTFLLLSFSEATEILVGGKANAWKLPSSQSQSLNQWAETSRFRVGDTLVW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
           +YEEGKDSVLKV KEDYEACNT+NPEQ FEDGNTKV+LHKPGPFYFISGA GHCEQGQKL
Sbjct: 61  SYEEGKDSVLKVGKEDYEACNTQNPEQRFEDGNTKVQLHKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           +VVVVTPRRRF+  SPAPSPAE EGPAV+PSSGAA+  VGFLA +VGILG+GVG L
Sbjct: 121 VVVVVTPRRRFVSFSPAPSPAEIEGPAVSPSSGAASFNVGFLAAVVGILGLGVGFL 176

[Close]

BLAST of Lsi08G012510 vs. NCBI nr
Match: XP_022962176.1 (early nodulin-like protein 1 [Cucurbita moschata])

HSP 1 Score: 306.2 bits (783), Expect = 2.4e-79
Identity = 152/176 (86.36%), Postives = 159/176 (90.34%), Query Frame = 0


Query: 59  MAGFSRALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVW 118
           MAG SRALFLLTF LFSFSEA EILVGGK NAWK+PSSQSQSLNQWAETSRFRVGDTL W
Sbjct: 1   MAGSSRALFLLTFLLFSFSEATEILVGGKPNAWKLPSSQSQSLNQWAETSRFRVGDTLAW 60

Query: 119 NYEEGKDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKL 178
           +YEEGKDSVLKV KEDYEACNT+NPEQ FEDGNTKVELHKPGPFYFISGA GHCEQGQKL
Sbjct: 61  SYEEGKDSVLKVGKEDYEACNTQNPEQRFEDGNTKVELHKPGPFYFISGAKGHCEQGQKL 120

Query: 179 IVVVVTPRRRFIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
           +VVVVTPRRRF+  SPAPSP E EGPAVAPSSGAA+  VGFLA  VGILG+GVG L
Sbjct: 121 VVVVVTPRRRFVSFSPAPSPTEIEGPAVAPSSGAASFNVGFLAAAVGILGLGVGFL 176

[Close]

BLAST of Lsi08G012510 vs. TAIR 10
Match: AT4G31840.1 (early nodulin-like protein 15 )

HSP 1 Score: 179.9 bits (455), Expect = 2.4e-45
Identity = 89/161 (55.28%), Postives = 115/161 (71.43%), Query Frame = 0


Query: 69  LTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGKDSVL 128
           ++ F FS   A E+ VGGKS  WKIP S S S N+WA+ +RF+VGD +V+ YE GKDSVL
Sbjct: 14  ISVFFFSSVNANEVTVGGKSGDWKIPPSSSFSFNEWAQKARFKVGDFIVFKYEAGKDSVL 73

Query: 129 KVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVTPRRR 188
           +V +E YE CNT +P+  + DGNTKV+L + GP YF+SG  GHC++GQKL +VV+TPR  
Sbjct: 74  QVTREAYEKCNTTSPKASYTDGNTKVKLDQAGPVYFVSGTEGHCQKGQKLRLVVITPRNS 133

Query: 189 FIGISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGV 230
               SP PSP+E +GPAVAP+SGAA L  GF  V   +LG+
Sbjct: 134 --AFSPGPSPSEFDGPAVAPTSGAAKLAGGFSVVFGLVLGL 172

[Close]

BLAST of Lsi08G012510 vs. TAIR 10
Match: AT2G25060.1 (early nodulin-like protein 14 )

HSP 1 Score: 176.8 bits (447), Expect = 2.0e-44
Identity = 91/164 (55.49%), Postives = 120/164 (73.17%), Query Frame = 0


Query: 65  ALFLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGK 124
           A+F L  FLFS + A E+ VGGKS  WKIP S S S  +WA+ +RF+VGD +V+ YE GK
Sbjct: 15  AIFSL-IFLFSLAAANEVTVGGKSGDWKIPPSSSYSFTEWAQKARFKVGDFIVFRYESGK 74

Query: 125 DSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVT 184
           DSVL+V KE Y +CNT NP   + DG TKV+L + GPFYFISGANGHCE+GQKL +VV++
Sbjct: 75  DSVLEVTKEAYNSCNTTNPLANYTDGETKVKLDRSGPFYFISGANGHCEKGQKLSLVVIS 134

Query: 185 PRRRFIGISPAPSPAESE-GPAVAPSSGAANLKVGFLAVIVGIL 228
           PR     ISPAPSP E E GPA+AP+  + ++++G   V++G++
Sbjct: 135 PRHSV--ISPAPSPVEFEDGPALAPAPISGSVRLGGCYVVLGLV 175

[Close]

BLAST of Lsi08G012510 vs. TAIR 10
Match: AT5G25090.1 (early nodulin-like protein 13 )

HSP 1 Score: 159.5 bits (402), Expect = 3.4e-39
Identity = 89/175 (50.86%), Postives = 120/175 (68.57%), Query Frame = 0


Query: 67  FLLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGKDS 126
           FL+ F L +   ++EI+VGGK+++WKIPSS S+SLN+WAE+ RFRVGDTLVW Y+E KDS
Sbjct: 11  FLIFFLLTNLVCSKEIIVGGKTSSWKIPSSPSESLNKWAESLRFRVGDTLVWKYDEEKDS 70

Query: 127 VLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVTPR 186
           VL+V K+ Y  CNT NP   + +G+TKV+L + GP++FISG+  +C +G+KL +VV++ R
Sbjct: 71  VLQVTKDAYINCNTTNPAANYSNGDTKVKLERSGPYFFISGSKSNCVEGEKLHIVVMSSR 130

Query: 187 RRFIG-------ISPAPSPAESEGPAVAPSSGAANLKVGFLAVIVGILGVGVGLL 235
               G        SPAPSPA    P VAP+SG +      L   VG+LG  VGLL
Sbjct: 131 GGHTGGFFTGSSPSPAPSPALLGAPTVAPASGGS---ASSLTRQVGVLGF-VGLL 181

[Close]

BLAST of Lsi08G012510 vs. TAIR 10
Match: AT5G57920.1 (early nodulin-like protein 10 )

HSP 1 Score: 146.0 bits (367), Expect = 3.9e-35
Identity = 74/147 (50.34%), Postives = 100/147 (68.03%), Query Frame = 0


Query: 68  LLTFFLFSFSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEGKDSV 127
           LL F L   SE REILVGGKSN WK P S+ ++LNQW+  +RF++GD+L+W Y    DSV
Sbjct: 11  LLLFGL--LSEGREILVGGKSNTWKAPESRDETLNQWSGRTRFKIGDSLLWKYNAENDSV 70

Query: 128 LKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVVTP-R 187
           L+VR+ DYE C+   P + ++DG+T +EL + GPFYFISG  GHC++G+KL VVV++P  
Sbjct: 71  LQVRQTDYERCDRSEPIRGYKDGHTNIELKRSGPFYFISGEEGHCQRGEKLRVVVLSPNH 130

Query: 188 RRFIGISPAP-----SPAESEGPAVAP 209
            R +  +PAP     SP  +   A AP
Sbjct: 131 NRSVVDAPAPVNIVLSPNYNRSVAAAP 155

[Close]

BLAST of Lsi08G012510 vs. TAIR 10
Match: AT4G30590.1 (early nodulin-like protein 12 )

HSP 1 Score: 144.4 bits (363), Expect = 1.1e-34
Identity = 83/179 (46.37%), Postives = 111/179 (62.01%), Query Frame = 0


Query: 69  LTFFLFS-----FSEAREILVGGKSNAWKIPSSQSQSLNQWAETSRFRVGDTLVWNYEEG 128
           L F LF+      S  R ILVGG   +WK+P S + +LN WAE +RF+VGD +VW Y+  
Sbjct: 10  LVFLLFAKVSHGASNPRVILVGGSVGSWKVPDSPNNTLNHWAENNRFKVGDFIVWKYDMK 69

Query: 129 KDSVLKVRKEDYEACNTENPEQIFEDGNTKVELHKPGPFYFISGANGHCEQGQKLIVVVV 188
            DSVL+V KEDYE+CNT NP + + DGNTKV L K GP++FISGA G+C +G+K+ +VV+
Sbjct: 70  VDSVLQVTKEDYESCNTANPLKQYNDGNTKVALDKSGPYFFISGAPGNCAKGEKITLVVL 129

Query: 189 TPRRRFIG-----------ISP-APSPAESEGPAVAPSSGAANLKVG----FLAVIVGI 227
             R+   G           +SP A +PA + GPA A  + A  LKV       AV+VG+
Sbjct: 130 AERKSGGGSSSGDAPKVSPVSPTAQTPAPAPGPAAA-HNAAVGLKVASGWFLTAVVVGL 187

The following BLAST results are available for this feature:

BLAST of Lsi08G012510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SK27	2.9e-43	55.49	Early nodulin-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At2g25060 PE=2 S...	[more]
Q8LC95	4.8e-38	50.86	Early nodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=At5g25090 PE=2 S...	[more]
Q02917	4.6e-25	39.53	Early nodulin-55-2 OS=Glycine max OX=3847 GN=ENOD55-2 PE=2 SV=1	[more]
Q9T076	3.9e-24	43.45	Early nodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g27520 PE=1 S...	[more]
P93329	6.9e-21	35.96	Early nodulin-20 OS=Medicago truncatula OX=3880 GN=ENOD20 PE=3 SV=1	[more]

BLAST of Lsi08G012510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VC90	2.7e-84	92.05	Early nodulin-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca...	[more]
A0A1S3BBT8	2.7e-84	92.05	early nodulin-like protein 1 OS=Cucumis melo OX=3656 GN=LOC103488354 PE=4 SV=1	[more]
A0A0A0LQG0	4.0e-80	88.76	Phytocyanin-like arabinogalactan-protein OS=Cucumis sativus OX=3659 GN=Csa_2G382...	[more]
A0A6J1K2Z5	6.8e-80	85.80	early nodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111491916 PE=4 SV...	[more]
A0A6J1HCD3	1.2e-79	86.36	early nodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111462709 PE=4 ...	[more]

BLAST of Lsi08G012510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884215.1	5.5e-84	92.98	early nodulin-like protein 1 [Benincasa hispida]	[more]
XP_008445271.1	5.5e-84	92.05	PREDICTED: early nodulin-like protein 1 [Cucumis melo] >KAA0064884.1 early nodul...	[more]
XP_004138700.1	8.2e-80	88.76	early nodulin-like protein 1 [Cucumis sativus] >KGN62997.1 hypothetical protein ...	[more]
XP_022996782.1	1.4e-79	85.80	early nodulin-like protein 1 [Cucurbita maxima]	[more]
XP_022962176.1	2.4e-79	86.36	early nodulin-like protein 1 [Cucurbita moschata]	[more]

BLAST of Lsi08G012510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31840.1	2.4e-45	55.28	early nodulin-like protein 15	[more]
AT2G25060.1	2.0e-44	55.49	early nodulin-like protein 14	[more]
AT5G25090.1	3.4e-39	50.86	early nodulin-like protein 13	[more]
AT5G57920.1	3.9e-35	50.34	early nodulin-like protein 10	[more]
AT4G30590.1	1.1e-34	46.37	early nodulin-like protein 12	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 78..186 e-value: 2.0E-33 score: 116.7
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 79..183
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 91..176 e-value: 2.8E-26 score: 91.3
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 80..184 score: 38.707726
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 53..230
None	No IPR available	PANTHER	PTHR33021:SF392	EARLY NODULIN-LIKE PROTEIN 3	coord: 53..230
IPR041846	Early nodulin-like protein domain	CDD	cd11019	OsENODL1_like	coord: 80..182 e-value: 2.93602E-57 score: 175.534

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G012510.1	Lsi08G012510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity