Lsi08G011700 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G011700
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAmmonium transporter
Locationchr08: 20318908 .. 20322284 (+)
RNA-Seq ExpressionLsi08G011700
SyntenyLsi08G011700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCAATATTATAGTCCCTCTGCCTTCCAACCTTCTCCCAGATGCAGCAAATCCAGAATGGATGAGCAAAGGCGACAACGCATGGCAGTTGACGGCGGCGACACTGGTCGGATTACAGAGCGTCCCGGGTCTAATAATCCTGTACGGCGGAGCGGTTAAGAAGAAATGGGCCATAAACTCAGCGTTCATGGCTCTGTACGCGTTCGCTTGCGTGTTGGTTTGTTGGGTTGGGTGGGGGTACCGGCTGTCGTTTGGGGATAAATTGGCGCCGTTTTGGGGGAAATTGAATGTGGCTTTGGATCAGGAGTTTCTGCTAGAGCAAGCTTTTGGTGGGAAGTTTCCATCGGCTACAATGGTGTTTTTTCAATTTGTATTTGCAGCGATTACTTTGGTTTTGATTGCTGGGGCTTTGCTTGGGCGTATGAATTTTTATGCTTGGATGTTGTTTGTGCCGCTTTGGTTAACGTTTTCTTATACTTTTGTGGCGTTTAGTATTTGGAGTCCTAATGGATTTTTCTTTAAAATGGGACTTATTGATTACTCTGGTGGTTACGTCATTCATTTGTCCTCTGGTGTCGCTGGCTTCACCGCTGCTTACTGGGTATGTTTTTTTTTTTAATACATTTTTTTTTTACTCTCTCGTTTCAATTCTAAATTTTGTTTAATTCCTATCAATTTTGTTTTTTTAAGCTTTTAGAAGGTTTAATTTTATCTCAATTTTATCTCTAGATTTTAAAAATTACACAATTAGGCACTATTTTGGCTCAATTTCAATTTTCAATTGTTGGGCTCATTTAAGCTCGTTTATACATAAAAAAAAAATTATCACATGACAATTTTTTATTGAACAACTCCTTAAGCGGCACAAAGATAGGTACAGCCTTGCAACACTCAAGTATTTTTCCTAATTATAATAGTTGTATTTTCAACTATTATAACTTATTATTAATAACTATATTACTATTTCAAATCTCTCTTACAATATTTACTTGTTTACTATTTCTTACTAGTCGACTCTTTCTTTCTTTCCTGAAGTGTTTACTATTTTCTACACATTCTCTCTTTCCATCTTTACTACCATGTGTATTTGCTACCCAAACTAAAATAGTTTGCATAGTGTTTATTATTTTCTACCAAAATTAAAACAATTTGCATCCCAAACACAAACATTATAATTGAGACTAAAATAGTCTGCACCCTAAACACAAACTATTATAACATACATGTTATAATAACCAACTCAATACTCTAAATACCTCCTTAGTTTTTAAGCTTTGAGACACTATATTTTTAAGTTAAAGAATCTTTAAACATGTACTTGTAAATTTTTGGATTATACCAAGTTACATACTTGAATTTAGTCTCTAAACCTTTGAACGTCTAATTTTGTGTTTAATGCATATTTAAACTTTAAAAGGTATGTAAATGGTTCTTAAAACTTTTAGTTTCTTATTTAGTAAGTTATTAACATACTATTTGATATTTTTTTTAAAAAAAATCACAGATATACTTGACACAAAATTGAAAATTTAAACTCCGTCTTACAAGAACAGTTGGTTTTAATTTTTAAATTTTGTATTTATTGTATCTTATTTGCTTTACGATGAATTTTATTTTTTTAAGAAAACATTTCAAATTTTAGTAAAATTTTAAAAATAAAAATTATTCTAAAATCATGTTCATGTCTTTTAAAATGTTATTTTCAAATATAGAAAAATGAACCAAAATATTTATAAATATAGCAAAATTTCACTGTCTATTTGTGATAGACTGCGATAGACTACTATTTATAAATACACATGACTGAAAGTTATATGTTTGAATACCTTAAAAATTTAAGGATCAAATAAACACAAATTAAAAAGTTTGAGGTCTAAATTTATAATTAAAGCTAAATTATGAATTAAAAATTGAGTTTTATAGTCGTAATTTGAAAATGGAAAATGTGTGAGTGACAGGTTGGGCCACGGCTTTCGAAGGACAGAGAAAGCTTCCCTCCGAACAACATTCTTCTGATGCTGGCCGGAGCAGGGCTGCTGTGGATGGGTTGGACCGGGTTCAACGGCGGAGACCCATACGCCGCCAACATCGATGCCTCACTGGCGGTTCTGAACACCCACGTTTGCACCGCCACCAGCCTCCTCACTTGGCTCATCTTCGACATCGTCTTCTTCAGAAAGCCCTCCGTCATCGGCGCCGTCCAGGGCATGATCACCGGCCTGGTTTGCATCACTCCCGCCGCAGGTCCCTTCTCTTTTTATTTACCCTCTTTCTCTATTATGAATATTTTAAACACCGTGCTAATTGCAATTTTACCATAAGTTTGATTTGTAGTTTCTAATTTTGAAAGTTTATAAACACTATTTTTATATATAAATTTCAATATTTTATTATCTATTTTTTTACTAATATTTTAATAAACCAAGCAAAGTTTCAATCTATACTTAATAAAAAAAAATTAAACGTATTTTTATTATTGACTAAATTGAAATCAAATTCAAAACATACTAAACTTTAAACGTTTAACAATTTAAAATCTATGAACCAAATGCAAACTAAACCCAAAACCTAGAGATCAAAAAGACTTTTTTTTTCTTTAAAAAAAACAATAAATAAAGTTCAAACTTTATAAGAATGAAATTCCTAATTTAACCTACAATATAAATAGTAAGTTCCTAAAGGTTTTTTTAAATTAAATAGGGGAGTAACATTATTGTAAAAATTTGTGAAACATAAAATATCCAGGAGTTGTTCAAGGATGGGCAGCGATTATAATGGGAGTGCTGTCAGGTTCAATCCCATGGTTCACAATGATGGTAGTTCACAAGAAATCCGCCATTCTCCAAAAGGTAGACGACACAATGGCCGTCCTCCACACCCACGCCATCGCCGGCAGCCTCGGCGGCATTTTAAGTGGCATCTTCGCCGAGCCACGCCTCAACTTCGTCTTCTACGGCGCCTACAACAAATACATCGGCCTTTTCTACGGCTTCAACACAAAACAAGTTAAACTCGGCTTTCGCCAACTTGGCGTTCAACTCCTCGGAATCGTCTTCGTCACTCTCATAAACGTCATTGTCACTAGCCTTATCTGTGTCCTCCTCCAGTACATCGTCCCGCTCCGAATGTCCGAGGAGGACATGGCTATAGGCGACGAGGCTGCCCACGGCGAAGAGGCCTACGCCATTTGGGGCCAAGGCGAGAAGATGGATTCTTCTAAGTATGATATTGAAGTCCCGGTTGCAACTAAACATAGGCATACAGGCCAGGTTGAAATGGCCTAACGCGATTGGTTTTTTCTATTTTTTTATGTAATTGCAGGGGTGAGAGATCAAACTATTTGTCACTACAATGGTAATTGATATCTTGTTTTAG

mRNA sequence

ATGAGCAATATTATAGTCCCTCTGCCTTCCAACCTTCTCCCAGATGCAGCAAATCCAGAATGGATGAGCAAAGGCGACAACGCATGGCAGTTGACGGCGGCGACACTGGTCGGATTACAGAGCGTCCCGGGTCTAATAATCCTGTACGGCGGAGCGGTTAAGAAGAAATGGGCCATAAACTCAGCGTTCATGGCTCTGTACGCGTTCGCTTGCGTGTTGGTTTGTTGGGTTGGGTGGGGGTACCGGCTGTCGTTTGGGGATAAATTGGCGCCGTTTTGGGGGAAATTGAATGTGGCTTTGGATCAGGAGTTTCTGCTAGAGCAAGCTTTTGGTGGGAAGTTTCCATCGGCTACAATGGTGTTTTTTCAATTTGTATTTGCAGCGATTACTTTGGTTTTGATTGCTGGGGCTTTGCTTGGGCGTATGAATTTTTATGCTTGGATGTTGTTTGTGCCGCTTTGGTTAACGTTTTCTTATACTTTTGTGGCGTTTAGTATTTGGAGTCCTAATGGATTTTTCTTTAAAATGGGACTTATTGATTACTCTGGTGGTTACGTCATTCATTTGTCCTCTGGTGTCGCTGGCTTCACCGCTGCTTACTGGGTTGGGCCACGGCTTTCGAAGGACAGAGAAAGCTTCCCTCCGAACAACATTCTTCTGATGCTGGCCGGAGCAGGGCTGCTGTGGATGGGTTGGACCGGGTTCAACGGCGGAGACCCATACGCCGCCAACATCGATGCCTCACTGGCGGTTCTGAACACCCACGTTTGCACCGCCACCAGCCTCCTCACTTGGCTCATCTTCGACATCGTCTTCTTCAGAAAGCCCTCCGTCATCGGCGCCGTCCAGGGCATGATCACCGGCCTGGTTTGCATCACTCCCGCCGCAGGAGTTGTTCAAGGATGGGCAGCGATTATAATGGGAGTGCTGTCAGGTTCAATCCCATGGTTCACAATGATGGTAGTTCACAAGAAATCCGCCATTCTCCAAAAGGTAGACGACACAATGGCCGTCCTCCACACCCACGCCATCGCCGGCAGCCTCGGCGGCATTTTAAGTGGCATCTTCGCCGAGCCACGCCTCAACTTCGTCTTCTACGGCGCCTACAACAAATACATCGGCCTTTTCTACGGCTTCAACACAAAACAAGTTAAACTCGGCTTTCGCCAACTTGGCGTTCAACTCCTCGGAATCGTCTTCGTCACTCTCATAAACGTCATTGTCACTAGCCTTATCTGTGTCCTCCTCCAGTACATCGTCCCGCTCCGAATGTCCGAGGAGGACATGGCTATAGGCGACGAGGCTGCCCACGGCGAAGAGGCCTACGCCATTTGGGGCCAAGGCGAGAAGATGGATTCTTCTAAGTATGATATTGAAGTCCCGGTTGCAACTAAACATAGGCATACAGGCCAGGTTGAAATGGCCTAACGCGATTGGTTTTTTCTATTTTTTTATGTAATTGCAGGGGTGAGAGATCAAACTATTTGTCACTACAATGGTAATTGATATCTTGTTTTAG

Coding sequence (CDS)

ATGAGCAATATTATAGTCCCTCTGCCTTCCAACCTTCTCCCAGATGCAGCAAATCCAGAATGGATGAGCAAAGGCGACAACGCATGGCAGTTGACGGCGGCGACACTGGTCGGATTACAGAGCGTCCCGGGTCTAATAATCCTGTACGGCGGAGCGGTTAAGAAGAAATGGGCCATAAACTCAGCGTTCATGGCTCTGTACGCGTTCGCTTGCGTGTTGGTTTGTTGGGTTGGGTGGGGGTACCGGCTGTCGTTTGGGGATAAATTGGCGCCGTTTTGGGGGAAATTGAATGTGGCTTTGGATCAGGAGTTTCTGCTAGAGCAAGCTTTTGGTGGGAAGTTTCCATCGGCTACAATGGTGTTTTTTCAATTTGTATTTGCAGCGATTACTTTGGTTTTGATTGCTGGGGCTTTGCTTGGGCGTATGAATTTTTATGCTTGGATGTTGTTTGTGCCGCTTTGGTTAACGTTTTCTTATACTTTTGTGGCGTTTAGTATTTGGAGTCCTAATGGATTTTTCTTTAAAATGGGACTTATTGATTACTCTGGTGGTTACGTCATTCATTTGTCCTCTGGTGTCGCTGGCTTCACCGCTGCTTACTGGGTTGGGCCACGGCTTTCGAAGGACAGAGAAAGCTTCCCTCCGAACAACATTCTTCTGATGCTGGCCGGAGCAGGGCTGCTGTGGATGGGTTGGACCGGGTTCAACGGCGGAGACCCATACGCCGCCAACATCGATGCCTCACTGGCGGTTCTGAACACCCACGTTTGCACCGCCACCAGCCTCCTCACTTGGCTCATCTTCGACATCGTCTTCTTCAGAAAGCCCTCCGTCATCGGCGCCGTCCAGGGCATGATCACCGGCCTGGTTTGCATCACTCCCGCCGCAGGAGTTGTTCAAGGATGGGCAGCGATTATAATGGGAGTGCTGTCAGGTTCAATCCCATGGTTCACAATGATGGTAGTTCACAAGAAATCCGCCATTCTCCAAAAGGTAGACGACACAATGGCCGTCCTCCACACCCACGCCATCGCCGGCAGCCTCGGCGGCATTTTAAGTGGCATCTTCGCCGAGCCACGCCTCAACTTCGTCTTCTACGGCGCCTACAACAAATACATCGGCCTTTTCTACGGCTTCAACACAAAACAAGTTAAACTCGGCTTTCGCCAACTTGGCGTTCAACTCCTCGGAATCGTCTTCGTCACTCTCATAAACGTCATTGTCACTAGCCTTATCTGTGTCCTCCTCCAGTACATCGTCCCGCTCCGAATGTCCGAGGAGGACATGGCTATAGGCGACGAGGCTGCCCACGGCGAAGAGGCCTACGCCATTTGGGGCCAAGGCGAGAAGATGGATTCTTCTAAGTATGATATTGAAGTCCCGGTTGCAACTAAACATAGGCATACAGGCCAGGTTGAAATGGCCTAA

Protein sequence

MSNIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQVEMA
Homology
BLAST of Lsi08G011700 vs. ExPASy Swiss-Prot
Match: A0A072VHJ1 (Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 5.2e-217
Identity = 364/477 (76.31%), Postives = 415/477 (87.00%), Query Frame = 0

Query: 6   VPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMA 65
           VP PSNLLP  ++PEW+SK DNAWQL AATLVG+QSVPGLIILYGGAVKKKWA+NSAFM+
Sbjct: 4   VPFPSNLLPSPSSPEWLSKADNAWQLMAATLVGMQSVPGLIILYGGAVKKKWAVNSAFMS 63

Query: 66  LYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFFQFV 125
           LYAFACV  CWV W YR+SFGD L PFWGK  +ALD+++L +QAF G FP+ATM++FQ V
Sbjct: 64  LYAFACVFFCWVFWAYRMSFGDTLFPFWGKPALALDEKYLFKQAFLGAFPNATMIYFQCV 123

Query: 126 FAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYSGGY 185
           FAAITL+LIAGA+LGRMNFYAWM+FVPLWLTFSYTF AFSIWS NGF  KMG+IDYSGGY
Sbjct: 124 FAAITLILIAGAVLGRMNFYAWMMFVPLWLTFSYTFTAFSIWSTNGFLAKMGIIDYSGGY 183

Query: 186 VIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANI 245
           VIHLSSGVAGFTAAYWVGPRL+KDRE FPPNN+LLMLAGAGLLWMGWTGFNGGDPY+  +
Sbjct: 184 VIHLSSGVAGFTAAYWVGPRLNKDRERFPPNNLLLMLAGAGLLWMGWTGFNGGDPYSVGL 243

Query: 246 DASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAAI 305
           DASLAVLNTH CTATSLLTW+  D++FFRKPSVIGAVQGMITGLVCITPAAGVVQGWAA+
Sbjct: 244 DASLAVLNTHACTATSLLTWVFLDVIFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAAL 303

Query: 306 IMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLNFVF 365
           IMG+ SGSIPWFTMMV+HK+S +LQKVDDTMAVLHTHAIAGSLGGIL+G+FAEP+LN +F
Sbjct: 304 IMGLFSGSIPWFTMMVIHKRSKLLQKVDDTMAVLHTHAIAGSLGGILTGLFAEPKLNSLF 363

Query: 366 YGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPLRMS 425
           YG YNKY+GLFYG        G RQ+G+QLLGI+FV  +NV+ TSLIC+ ++  VPLRMS
Sbjct: 364 YGVYNKYVGLFYGIQMNMASTGRRQIGIQLLGILFVIFVNVVSTSLICLFIRLFVPLRMS 423

Query: 426 EEDMAIGDEAAHGEEAYAIWGQGEKMD--SSKY------DIEVPVATKHRHTGQVEM 475
           EEDM IGDEAAHGEEAYAIWGQG+K +  +SKY      D+EV   TK++   Q+EM
Sbjct: 424 EEDMEIGDEAAHGEEAYAIWGQGDKHENSNSKYGSSLYEDVEVAATTKNKRGSQIEM 480

BLAST of Lsi08G011700 vs. ExPASy Swiss-Prot
Match: G7L1W7 (Ammonium transporter 2 member 4 OS=Medicago truncatula OX=3880 GN=AMT2-4 PE=2 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 1.1e-171
Identity = 298/453 (65.78%), Postives = 369/453 (81.46%), Query Frame = 0

Query: 8   LPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALY 67
           LPSNLLPD A+PEWM+KGDNAWQLTAAT+VGLQS+PGL+ILYG  VKK WAINSAFMA Y
Sbjct: 3   LPSNLLPDEASPEWMNKGDNAWQLTAATMVGLQSIPGLVILYGSLVKKTWAINSAFMAFY 62

Query: 68  AFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFFQFVFA 127
           AFA VL+CWV W Y++SFG+K+  F GK NVALD++FLL +AF G FP+ATMVF+Q VFA
Sbjct: 63  AFASVLLCWVSWAYQMSFGEKMVFFLGKPNVALDEKFLLGKAFLGNFPNATMVFYQGVFA 122

Query: 128 AITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYSGGYVI 187
            +TL+LIAGALLGRMN  AWMLFVPLW+TFSYT VAFSIW P+G+  K G+ID++GGYVI
Sbjct: 123 GLTLILIAGALLGRMNIRAWMLFVPLWVTFSYTVVAFSIWCPDGWLAKRGVIDFAGGYVI 182

Query: 188 HLSSGVAGFTAAYWVGPRLSKDRESFP--PNNILLMLAGAGLLWMGWTGFNGGDPYAANI 247
           HLS+GVAGFTAAYWVGPR  KDRE+FP   NN++++LAGAGLLWMGW+GFNGG P+ A+ 
Sbjct: 183 HLSAGVAGFTAAYWVGPRADKDRETFPAATNNMIMVLAGAGLLWMGWSGFNGGAPFVAST 242

Query: 248 DASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAAI 307
            ASLA+LNTHVCTA S+  W++ D  +F KP+V GAVQGMITGLVCITPAAGVVQGWAAI
Sbjct: 243 IASLAILNTHVCTAASITVWVMLDTFYFGKPTVFGAVQGMITGLVCITPAAGVVQGWAAI 302

Query: 308 IMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLNFVF 367
           +MG +SGSIPW+TMMV+H K   L+K+DD MAV HTHAIAG+LGGIL+G FA P+L  +F
Sbjct: 303 LMGFISGSIPWYTMMVLHNKVNFLKKIDDPMAVFHTHAIAGALGGILTGFFAVPKLCRLF 362

Query: 368 YGA--YNKYIGLFYGFNTK-QVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPL 427
           Y    + KYIGL YG   K   + G +Q+ +Q+  IVFV   NV++TSLIC++++ IVPL
Sbjct: 363 YMVPDWEKYIGLAYGLQNKGATQAGLKQMVIQIEAIVFVICYNVLMTSLICLIVRVIVPL 422

Query: 428 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSK 456
           R++ + + +GD+A HGE+A+A+  +  K  + K
Sbjct: 423 RLNGDALQMGDKAIHGEDAFALHSEATKFVNIK 455

BLAST of Lsi08G011700 vs. ExPASy Swiss-Prot
Match: Q84KJ6 (Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-1 PE=2 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 3.3e-171
Identity = 305/463 (65.87%), Postives = 359/463 (77.54%), Query Frame = 0

Query: 17  ANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCW 76
           A PEW++KGDNAWQ+ +ATLVG+QSVPGL+ILYG  VKKKWA+NSAFMALYAFA V +CW
Sbjct: 18  AVPEWLNKGDNAWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSAFMALYAFAAVWLCW 77

Query: 77  VGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAF-----------GGK------------ 136
           V WGY +SFG KL PFWGK   AL Q FLL QA            GG             
Sbjct: 78  VTWGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGGGADAVVETPWVNPL 137

Query: 137 FPSATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF 196
           +P ATMV+FQ VFAAITL+L+AG+LLGRMN  AWMLFVPLWLTFSYT  AFS+W   GF 
Sbjct: 138 YPMATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSYTVGAFSLWG-GGFL 197

Query: 197 FKMGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWT 256
           F  G++DYSGGYVIHLSSGVAGFTAAYWVGPR +KDRE FPPNN+LLML GAG+LWMGW 
Sbjct: 198 FHWGVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLLMLTGAGILWMGWA 257

Query: 257 GFNGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCIT 316
           GFNGGDPY+ANID+SLAVLNT++C ATSLL W   D++FF+KPSVIGAVQGMITGLVCIT
Sbjct: 258 GFNGGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIGAVQGMITGLVCIT 317

Query: 317 PAAGVVQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILS 376
           P AG+VQGWAAI+MG+LSGSIPWFTMMVVHK+S +LQ+VDDT+ V HTHA+AG LGG  +
Sbjct: 318 PGAGLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFHTHAVAGFLGGATT 377

Query: 377 GIFAEPRLNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLIC 436
           G+FAEP L  +F    N   G FY       + G  Q   Q+ G +F+   NV+VTSL+C
Sbjct: 378 GLFAEPVLCSLFLPVTNSR-GAFY-----PGRGGGLQFVRQVAGALFIICWNVVVTSLVC 437

Query: 437 VLLQYIVPLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKY 457
           + ++ +VPLRM EE++AIGD+A HGEEAYA+WG GEK DS+K+
Sbjct: 438 LAVRAVVPLRMPEEELAIGDDAVHGEEAYALWGDGEKYDSTKH 473

BLAST of Lsi08G011700 vs. ExPASy Swiss-Prot
Match: G7LAA8 (Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV=2)

HSP 1 Score: 591.3 bits (1523), Expect = 9.9e-168
Identity = 298/472 (63.14%), Postives = 361/472 (76.48%), Query Frame = 0

Query: 2   SNIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINS 61
           S  I  LP +LLP+ A+PEW +K DNAWQLTAATLVGLQ+VPGL+ILYG  VKKKWA+NS
Sbjct: 6   SKYISHLPESLLPNDASPEWNNKADNAWQLTAATLVGLQTVPGLVILYGSMVKKKWAVNS 65

Query: 62  AFMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKF-PSATMV 121
           AFMALYAFA VLVCWV W + ++FG KL PF GK N AL Q+FLL +A    + P A  V
Sbjct: 66  AFMALYAFAAVLVCWVLWAHHMAFGTKLLPFVGKPNFALSQKFLLSKASTNYYLPMADFV 125

Query: 122 FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLID 181
           F+QF FAAITLVL+ G+LLGRMNFYAWMLFVPLWLT SYT  AF+IW  NG F +  +ID
Sbjct: 126 FYQFAFAAITLVLLGGSLLGRMNFYAWMLFVPLWLTLSYTVGAFTIWG-NG-FLEGKIID 185

Query: 182 YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP 241
           Y+GG+VIHLSSGVAGFTAAYWVGPR S DR++FPPNNI+ ML GAG LWMGWTGFNGG P
Sbjct: 186 YAGGFVIHLSSGVAGFTAAYWVGPRTSNDRQNFPPNNIIHMLGGAGFLWMGWTGFNGGAP 245

Query: 242 YAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQ 301
           +      SLA+ NTH+CTATS+L W+  D+  ++K S+IG+VQGM+TGLVCITP AG+V 
Sbjct: 246 FQVGEITSLAIFNTHLCTATSILVWISLDMAVYKKGSLIGSVQGMMTGLVCITPGAGLVD 305

Query: 302 GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPR 361
            WAAI+MG LSGSIPW+TMMV+HKKS   Q VDDT+ V HTHA+AG LGGILSG+FA+P+
Sbjct: 306 PWAAILMGALSGSIPWYTMMVLHKKSPFFQSVDDTLGVFHTHAVAGILGGILSGVFAKPK 365

Query: 362 LNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIV 421
           L  + YG Y    GL Y +    +  G +Q+  QLLG VF+T+ NV++TSLIC+LL   V
Sbjct: 366 LLRILYGPYGS--GLLYSYFDDNIGQGIKQMWYQLLGAVFITIWNVVITSLICILLNRFV 425

Query: 422 PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMD-SSKYDIE--VPVATKHRHT 470
            LRM EED+ +GD+AAHGEEAY +WG GE+M    + DI   +P  +  RH+
Sbjct: 426 NLRMQEEDLEVGDDAAHGEEAYVLWGDGERMRLPLRRDISPIIPYISHQRHS 473

BLAST of Lsi08G011700 vs. ExPASy Swiss-Prot
Match: Q69T29 (Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-3 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 5.4e-166
Identity = 287/470 (61.06%), Postives = 361/470 (76.81%), Query Frame = 0

Query: 6   VPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMA 65
           +P+     P  ++P+W++KGDNAWQ+T+ATLVGLQS+PGL+ILYG  VKKKWAINSAFMA
Sbjct: 6   IPMAYQTTP--SSPDWLNKGDNAWQMTSATLVGLQSMPGLVILYGSIVKKKWAINSAFMA 65

Query: 66  LYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQA---------------- 125
           LYAFA V +CWV W Y +SFGD+L PFWGK   AL Q FL+ Q+                
Sbjct: 66  LYAFAAVWICWVVWAYNMSFGDRLLPFWGKARPALGQSFLVAQSELTATAIRYHNGSAEA 125

Query: 126 --FGGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIW 185
                 +P ATMV+FQ +FA+IT++++AG+LLGRMN  AWM FVPLW+TFSYT  AFS+W
Sbjct: 126 PMLKPLYPVATMVYFQCMFASITIIILAGSLLGRMNIKAWMAFVPLWITFSYTVCAFSLW 185

Query: 186 SPNGFFFKMGLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGL 245
              GF F+ G+IDYSGGYVIHLSSG+AG TAAYWVGPR + DRE FPPNNILL+LAGAGL
Sbjct: 186 G-GGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRSASDRERFPPNNILLVLAGAGL 245

Query: 246 LWMGWTGFNGGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMIT 305
           LW+GWTGFNGGDPY+ANID+S+AVLNTH+C +TSLL W I D+ FF KPSVIGAVQGMIT
Sbjct: 246 LWLGWTGFNGGDPYSANIDSSMAVLNTHICASTSLLVWTILDVFFFGKPSVIGAVQGMIT 305

Query: 306 GLVCITPAAGVVQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGS 365
           GLVCITP AG+VQGWAAI+MG+LSGSIPW+TMMV+HKK + +Q++DDT+ V HTHA+AG 
Sbjct: 306 GLVCITPGAGLVQGWAAIVMGILSGSIPWYTMMVLHKKWSFMQRIDDTLGVFHTHAVAGF 365

Query: 366 LGGILSGIFAEPRLNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVI 425
           LGG  +G+FAEP L  +F    +   G FYG        G  Q G Q+ G +FVT  N++
Sbjct: 366 LGGATTGLFAEPILCSLFLSIPDSK-GAFYG------GPGGSQFGKQIAGALFVTAWNIV 425

Query: 426 VTSLICVLLQYIVPLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYD 458
           +TS+ICV++  I+PLR++++++ IGD+A HGEEAYAIW +GE  D + ++
Sbjct: 426 ITSIICVIISLILPLRIADQELLIGDDAVHGEEAYAIWAEGELNDMTHHN 465

BLAST of Lsi08G011700 vs. ExPASy TrEMBL
Match: A0A6J1HD34 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111462995 PE=3 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 6.5e-263
Identity = 453/473 (95.77%), Postives = 464/473 (98.10%), Query Frame = 0

Query: 3   NIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSA 62
           ++IVPLPSNLLPD ANPEWMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 63  FMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFF 122
           FMALYAFACVLVCWVGWGYRLSFGDKL PFWGKLNVALDQ+FLLEQAF GK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 123 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 182
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 183 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 242
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 243 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 302
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAGVVQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 303 AAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLN 362
           AAIIMG+LSGSIPWFTMMVVHKKSAILQKVDDTMAV HTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 363 FVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPL 422
           FV+YGAYNKY+GLFYGFN+KQVKLGFRQLGVQLLGI+FVTLIN+IVTSLICVLLQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 423 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQVEMA 476
           RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPV  KHRH G VEMA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHAGHVEMA 474

BLAST of Lsi08G011700 vs. ExPASy TrEMBL
Match: A0A6J1K7F6 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111491842 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.5e-262
Identity = 452/473 (95.56%), Postives = 464/473 (98.10%), Query Frame = 0

Query: 3   NIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSA 62
           ++IVPLPSNLLPD ANPEWMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 63  FMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFF 122
           FMALYAFACVLVCWVGWGYRLSFGD+L PFWGKLNVALDQ+FLLEQAF GK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDRLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 123 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 182
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 183 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 242
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 243 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 302
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 303 AAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLN 362
           AAIIMG+LSGSIPWFTMMVVHKKSAILQKVDDTMAV HTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 363 FVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPL 422
           FV+YGAYNKY+GLFYGFN+KQVKLGFRQLGVQLLGI+FVTLIN+IVTSLICVLLQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 423 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQVEMA 476
           RMSEEDMAIGDEAAHGEEAYAIWGQGEKMD+SKYDIEVP   KHRH GQVEMA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDTSKYDIEVPAVAKHRHIGQVEMA 474

BLAST of Lsi08G011700 vs. ExPASy TrEMBL
Match: A0A1S3BCV9 (Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103488277 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 3.8e-255
Identity = 443/476 (93.07%), Postives = 460/476 (96.64%), Query Frame = 0

Query: 1   MSNIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAIN 60
           MSNIIVPLP+N++PD  +P WMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAIN
Sbjct: 1   MSNIIVPLPANVVPDIGDPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAIN 60

Query: 61  SAFMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMV 120
           SAFMALYAFACVLVCWVGWGYRLSFG+KL PFWGKLNVALDQE+LL+Q F GKFP+ATMV
Sbjct: 61  SAFMALYAFACVLVCWVGWGYRLSFGEKLVPFWGKLNVALDQEYLLQQGFAGKFPAATMV 120

Query: 121 FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLID 180
           FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSP GFF KMGLID
Sbjct: 121 FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPEGFFAKMGLID 180

Query: 181 YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP 240
           YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP
Sbjct: 181 YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP 240

Query: 241 YAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQ 300
           YAANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAGVVQ
Sbjct: 241 YAANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQ 300

Query: 301 GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPR 360
           GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEP 
Sbjct: 301 GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPH 360

Query: 361 LNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIV 420
           LN +FYG YN+YIGLFYGFNTKQV+LGFRQLGVQLLGI+FVTL+NVIVTSLICVLLQYIV
Sbjct: 361 LNNIFYGNYNQYIGLFYGFNTKQVRLGFRQLGVQLLGIIFVTLLNVIVTSLICVLLQYIV 420

Query: 421 PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPV-ATKHRH-TGQVEM 475
           PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYD+E P+ A KHRH  GQVEM
Sbjct: 421 PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDVEAPIAAAKHRHAAGQVEM 476

BLAST of Lsi08G011700 vs. ExPASy TrEMBL
Match: A0A6J1BQ83 (Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111004759 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.8e-250
Identity = 434/473 (91.75%), Postives = 455/473 (96.19%), Query Frame = 0

Query: 1   MSN--IIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWA 60
           MSN  IIVPLP+NLL DAA+P WMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWA
Sbjct: 1   MSNYSIIVPLPANLLEDAADPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWA 60

Query: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSAT 120
           INSAFMALYAFACVLVCWVGWGYRLSFGDKL PFWGKLNVALDQ+FLL+ AF GKFPSAT
Sbjct: 61  INSAFMALYAFACVLVCWVGWGYRLSFGDKLVPFWGKLNVALDQKFLLDPAFSGKFPSAT 120

Query: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGL 180
           MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFF+KMGL
Sbjct: 121 MVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFYKMGL 180

Query: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240
           IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG
Sbjct: 181 IDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGG 240

Query: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGV 300
           DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAG+
Sbjct: 241 DPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGL 300

Query: 301 VQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAE 360
           VQGWAAIIMG+LSGSIPWFTMMVVHKKS +LQKVDDTMAV HTHAIAGSLGGILSGIFAE
Sbjct: 301 VQGWAAIIMGMLSGSIPWFTMMVVHKKSPVLQKVDDTMAVFHTHAIAGSLGGILSGIFAE 360

Query: 361 PRLNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQY 420
           PRLN ++YGAYNKY+GLFYGF+T QV LGFRQLGVQLLGI+FVTLINVIVT++ICV LQY
Sbjct: 361 PRLNSIYYGAYNKYVGLFYGFSTAQVGLGFRQLGVQLLGIIFVTLINVIVTTIICVALQY 420

Query: 421 IVPLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQ 472
           IVPLRM+EEDMAIGDEAAHGEEAYAIWGQGEK+D+SKYDIE+P   KHRH  +
Sbjct: 421 IVPLRMTEEDMAIGDEAAHGEEAYAIWGQGEKLDNSKYDIEMP--AKHRHAAE 471

BLAST of Lsi08G011700 vs. ExPASy TrEMBL
Match: A0A6J5TRS8 (Ammonium transporter OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS9100 PE=3 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.1e-225
Identity = 384/473 (81.18%), Postives = 430/473 (90.91%), Query Frame = 0

Query: 7   PLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMAL 66
           PLPSNLLPD ANP+WM+K DNAWQLTAATLVGLQSVPGLIILYGGAVKKKWA+NSAFMAL
Sbjct: 8   PLPSNLLPDPANPDWMNKADNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAVNSAFMAL 67

Query: 67  YAFACVLVCWVGWGYRLSFGD---KLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFFQ 126
           YAFACVLVCWVGWGY +SFG+   K  PFWG+ +VAL+Q++LLEQAF GKFP+ATMV+FQ
Sbjct: 68  YAFACVLVCWVGWGYHMSFGETLIKAVPFWGRADVALNQKYLLEQAFSGKFPNATMVYFQ 127

Query: 127 FVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYSG 186
           FVFAAITL+LIAGALLGRMNFYAWMLFVPLWLTFSYT  AFSIWSPNGF FK GLIDYSG
Sbjct: 128 FVFAAITLILIAGALLGRMNFYAWMLFVPLWLTFSYTIGAFSIWSPNGFLFKKGLIDYSG 187

Query: 187 GYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAA 246
           G+VIHLSSGVAGFTAAYWVGPR +KDRE FPPNNILLML GAGLLWMGWTGFNGGDPY  
Sbjct: 188 GFVIHLSSGVAGFTAAYWVGPRSNKDRERFPPNNILLMLTGAGLLWMGWTGFNGGDPYVV 247

Query: 247 NIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGWA 306
           ++DASLAVLNTHVCTATSLLTWL+ D++FFRKPSVIGAVQGMITGLVCITPAAGVVQGWA
Sbjct: 248 SVDASLAVLNTHVCTATSLLTWLMLDLIFFRKPSVIGAVQGMITGLVCITPAAGVVQGWA 307

Query: 307 AIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLNF 366
           AI+MG+ SGSIPWFTMMVVHKKSA+LQKVDDTMAVLHTHAIAG+LGGIL+G+FA PRL F
Sbjct: 308 AILMGLFSGSIPWFTMMVVHKKSALLQKVDDTMAVLHTHAIAGALGGILTGLFAHPRLCF 367

Query: 367 VFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPLR 426
           +F  +Y +Y+GLFYGF    V  GFRQLG+QLLGI +V ++NV+VTSLICVL+Q+IVPLR
Sbjct: 368 LFTTSYGQYVGLFYGFKMGVVHTGFRQLGIQLLGIFYVVILNVLVTSLICVLIQFIVPLR 427

Query: 427 MSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRH-TGQVEMA 476
           MS+ DM IGDEAAHGEEAYAIWGQGEK+++S+Y  ++  +TK RH  GQ+EMA
Sbjct: 428 MSDADMEIGDEAAHGEEAYAIWGQGEKLENSRYGYDIQSSTKSRHAAGQIEMA 480

BLAST of Lsi08G011700 vs. NCBI nr
Match: XP_022962592.1 (ammonium transporter 3 member 1-like [Cucurbita moschata] >KAG6598591.1 Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029524.1 Ammonium transporter 3 member 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 916.0 bits (2366), Expect = 1.3e-262
Identity = 453/473 (95.77%), Postives = 464/473 (98.10%), Query Frame = 0

Query: 3   NIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSA 62
           ++IVPLPSNLLPD ANPEWMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 63  FMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFF 122
           FMALYAFACVLVCWVGWGYRLSFGDKL PFWGKLNVALDQ+FLLEQAF GK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 123 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 182
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 183 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 242
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 243 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 302
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAGVVQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 303 AAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLN 362
           AAIIMG+LSGSIPWFTMMVVHKKSAILQKVDDTMAV HTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 363 FVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPL 422
           FV+YGAYNKY+GLFYGFN+KQVKLGFRQLGVQLLGI+FVTLIN+IVTSLICVLLQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 423 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQVEMA 476
           RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPV  KHRH G VEMA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHAGHVEMA 474

BLAST of Lsi08G011700 vs. NCBI nr
Match: XP_023546976.1 (ammonium transporter 3 member 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 914.1 bits (2361), Expect = 5.1e-262
Identity = 452/473 (95.56%), Postives = 463/473 (97.89%), Query Frame = 0

Query: 3   NIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSA 62
           ++IVPLP NLLPD ANPEWMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPPNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 63  FMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFF 122
           FMALYAFACVLVCWVGWGYRLSFGDKL PFWGKLNVALDQ+FLLEQAF GK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDKLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 123 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 182
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 183 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 242
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 243 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 302
           ANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAGVVQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 303 AAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLN 362
           AAIIMG+LSGSIPWFTMMVVHKKSAILQKVDDTMAV HTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 363 FVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPL 422
           FV+YGAYNKY+GLFYGFN+KQVKLGFRQLGVQLLGI+FVTLIN+IVTSLICVLLQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 423 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQVEMA 476
           RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPV  KHRH G VEMA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVVAKHRHAGHVEMA 474

BLAST of Lsi08G011700 vs. NCBI nr
Match: XP_022996665.1 (ammonium transporter 3 member 1-like [Cucurbita maxima])

HSP 1 Score: 914.1 bits (2361), Expect = 5.1e-262
Identity = 452/473 (95.56%), Postives = 464/473 (98.10%), Query Frame = 0

Query: 3   NIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSA 62
           ++IVPLPSNLLPD ANPEWMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAINSA
Sbjct: 2   SLIVPLPSNLLPDPANPEWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAINSA 61

Query: 63  FMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFF 122
           FMALYAFACVLVCWVGWGYRLSFGD+L PFWGKLNVALDQ+FLLEQAF GK PSATMVFF
Sbjct: 62  FMALYAFACVLVCWVGWGYRLSFGDRLLPFWGKLNVALDQQFLLEQAFSGKLPSATMVFF 121

Query: 123 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 182
           QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS
Sbjct: 122 QFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYS 181

Query: 183 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 242
           GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA
Sbjct: 182 GGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYA 241

Query: 243 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 302
           ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW
Sbjct: 242 ANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGW 301

Query: 303 AAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLN 362
           AAIIMG+LSGSIPWFTMMVVHKKSAILQKVDDTMAV HTHAIAGSLGGILSGIFAEPRLN
Sbjct: 302 AAIIMGLLSGSIPWFTMMVVHKKSAILQKVDDTMAVFHTHAIAGSLGGILSGIFAEPRLN 361

Query: 363 FVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPL 422
           FV+YGAYNKY+GLFYGFN+KQVKLGFRQLGVQLLGI+FVTLIN+IVTSLICVLLQYIVPL
Sbjct: 362 FVYYGAYNKYVGLFYGFNSKQVKLGFRQLGVQLLGILFVTLINIIVTSLICVLLQYIVPL 421

Query: 423 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQVEMA 476
           RMSEEDMAIGDEAAHGEEAYAIWGQGEKMD+SKYDIEVP   KHRH GQVEMA
Sbjct: 422 RMSEEDMAIGDEAAHGEEAYAIWGQGEKMDTSKYDIEVPAVAKHRHIGQVEMA 474

BLAST of Lsi08G011700 vs. NCBI nr
Match: XP_038885846.1 (ammonium transporter 2 member 5-like [Benincasa hispida])

HSP 1 Score: 908.7 bits (2347), Expect = 2.1e-260
Identity = 450/475 (94.74%), Postives = 464/475 (97.68%), Query Frame = 0

Query: 1   MSNIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAIN 60
           MSNIIVPLPSNL+ D A+P+WMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAIN
Sbjct: 1   MSNIIVPLPSNLVTDTADPDWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAIN 60

Query: 61  SAFMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMV 120
           SAFMALYAFACVLVCWVGWGYRLSFGDKL PFWGKLNVAL+Q++LLEQ F GKFP+ATMV
Sbjct: 61  SAFMALYAFACVLVCWVGWGYRLSFGDKLTPFWGKLNVALEQQYLLEQGFAGKFPAATMV 120

Query: 121 FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLID 180
           FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSP GFF KMGLID
Sbjct: 121 FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPQGFFNKMGLID 180

Query: 181 YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP 240
           YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP
Sbjct: 181 YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP 240

Query: 241 YAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQ 300
           YAANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAGVVQ
Sbjct: 241 YAANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQ 300

Query: 301 GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPR 360
           GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSG+FAEPR
Sbjct: 301 GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGLFAEPR 360

Query: 361 LNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIV 420
           LN +FYG YNKYIGLFYGFNTKQVKLGFRQLGVQLLGI+FVTLIN+IVTSLICVLLQYIV
Sbjct: 361 LNLIFYGNYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIIFVTLINIIVTSLICVLLQYIV 420

Query: 421 PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRHTGQVEMA 476
           PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYD+EVPVATKHR  GQVEMA
Sbjct: 421 PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDMEVPVATKHRQAGQVEMA 475

BLAST of Lsi08G011700 vs. NCBI nr
Match: XP_004138867.1 (ammonium transporter 3 member 1 [Cucumis sativus] >KAE8652192.1 hypothetical protein Csa_021807 [Cucumis sativus])

HSP 1 Score: 893.3 bits (2307), Expect = 9.3e-256
Identity = 445/475 (93.68%), Postives = 458/475 (96.42%), Query Frame = 0

Query: 1   MSNIIVPLPSNLLPDAANPEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAIN 60
           MSNIIVPLP+NL+ D  +P WMSKGDNAWQ+TAATLVGLQSVPGLIILYGGAVKKKWAIN
Sbjct: 1   MSNIIVPLPANLVTDVGDPGWMSKGDNAWQMTAATLVGLQSVPGLIILYGGAVKKKWAIN 60

Query: 61  SAFMALYAFACVLVCWVGWGYRLSFGDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMV 120
           SAFMALYAFACVLVCWVGWGYRLSFG+KLAPFWGKLNVALDQE+LL   F GKFP+ATMV
Sbjct: 61  SAFMALYAFACVLVCWVGWGYRLSFGEKLAPFWGKLNVALDQEYLLAPGFAGKFPTATMV 120

Query: 121 FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLID 180
           FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSP GFF KMGLID
Sbjct: 121 FFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPGGFFAKMGLID 180

Query: 181 YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP 240
           YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP
Sbjct: 181 YSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDP 240

Query: 241 YAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQ 300
           YAANIDASLAVLNTHVCTATSLLTWLIFDI+FFRKPSVIGAVQGMITGLVCITPAAGVVQ
Sbjct: 241 YAANIDASLAVLNTHVCTATSLLTWLIFDIIFFRKPSVIGAVQGMITGLVCITPAAGVVQ 300

Query: 301 GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPR 360
           GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEP 
Sbjct: 301 GWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPH 360

Query: 361 LNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIV 420
           LN +FYG YNKYIGLFYGFNTKQVKLGFRQLGVQLLGI+FVTL+N+IVTSLICVLLQYIV
Sbjct: 361 LNNIFYGNYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIIFVTLLNIIVTSLICVLLQYIV 420

Query: 421 PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEVPVATKHRH-TGQVEM 475
           PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIE PV+ KHRH  GQVEM
Sbjct: 421 PLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKYDIEAPVSAKHRHAAGQVEM 475

BLAST of Lsi08G011700 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 555.4 bits (1430), Expect = 4.3e-158
Identity = 278/445 (62.47%), Postives = 346/445 (77.75%), Query Frame = 0

Query: 19  PEWMSKGDNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVG 78
           PEW++KGDNAWQLTAATLVGLQS+PGL+ILY   VKKKWA+NSAFMALYAFA VL+CWV 
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 79  WGYRLSFGDKLAPFWGKLNVALDQEFLLEQA-------FGGKFPSATMVFFQFVFAAITL 138
             Y+++FG++L PFWGK   A DQ +L  QA           FP AT+V+FQF FAAIT 
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVAAPYFPMATLVYFQFTFAAITT 132

Query: 139 VLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKMGLIDYSGGYVIHLSS 198
           +L+AG++LGRMN  AWM FVPLWL FSYT  A+SIW   GF ++ G+IDYSGGYVIHLSS
Sbjct: 133 ILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWG-GGFLYQWGVIDYSGGYVIHLSS 192

Query: 199 GVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFNGGDPYAANIDASLAV 258
           GVAGF AAYWVGPR   DRE FPPNN+LLMLAGAGLLWMGW+GFNGG PYAAN+ +S+AV
Sbjct: 193 GVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYAANLTSSIAV 252

Query: 259 LNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVVQGWAAIIMGVLS 318
           LNT++  ATSLL W   D++FF KPSVIGA+QGM+TGL  +TP AG++Q WAAII+GV+S
Sbjct: 253 LNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTWAAIIIGVVS 312

Query: 319 GSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFAEPRLNFVFYGAYNK 378
           G+ PW +MM++HKKSA+LQKVDDT+AV +THA+AG LGGI++G+FA P L  V       
Sbjct: 313 GTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDL-CVLVLPLPA 372

Query: 379 YIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQYIVPLRMSEEDMAI 438
             G FYG N      G +QL  QL G  F+ + NV+ T++I + ++  +PLRM+EE++ I
Sbjct: 373 TRGAFYGGN------GGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEELGI 432

Query: 439 GDEAAHGEEAYAIWGQGEKMDSSKY 457
           GD+AAHGEEAYA+WG GEK D++++
Sbjct: 433 GDDAAHGEEAYALWGDGEKFDATRH 449

BLAST of Lsi08G011700 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 424.1 bits (1089), Expect = 1.5e-118
Identity = 212/340 (62.35%), Postives = 268/340 (78.82%), Query Frame = 0

Query: 117 ATMVFFQFVFAAITLVLIAGALLGRMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGFFFKM 176
           AT+V+FQF FAAIT +L+AG++LGRMN  AWM FVPLWL FSYT  A+SIW   GF ++ 
Sbjct: 2   ATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWG-GGFLYQW 61

Query: 177 GLIDYSGGYVIHLSSGVAGFTAAYWVGPRLSKDRESFPPNNILLMLAGAGLLWMGWTGFN 236
           G+IDYSGGYVIHLSSGVAGF AAYWVGPR   DRE FPPNN+LLMLAGAGLLWMGW+GFN
Sbjct: 62  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 121

Query: 237 GGDPYAANIDASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAA 296
           GG PYAAN+ +S+AVLNT++  ATSLL W   D++FF KPSVIGA+QGM+TGL  +TP A
Sbjct: 122 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 181

Query: 297 GVVQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIF 356
           G++Q WAAII+GV+SG+ PW +MM++HKKSA+LQKVDDT+AV +THA+AG LGGI++G+F
Sbjct: 182 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLF 241

Query: 357 AEPRLNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLL 416
           A P L  V         G FYG N      G +QL  QL G  F+ + NV+ T++I + +
Sbjct: 242 AHPDL-CVLVLPLPATRGAFYGGN------GGKQLLKQLAGAAFIAVWNVVSTTIILLAI 301

Query: 417 QYIVPLRMSEEDMAIGDEAAHGEEAYAIWGQGEKMDSSKY 457
           +  +PLRM+EE++ IGD+AAHGEEAYA+WG GEK D++++
Sbjct: 302 RVFIPLRMAEEELGIGDDAAHGEEAYALWGDGEKFDATRH 333

BLAST of Lsi08G011700 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-25
Identity = 122/445 (27.42%), Postives = 195/445 (43.82%), Query Frame = 0

Query: 26  DNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 85
           DN + L +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 86  GDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFFQFVFAAITLVLIAGALLGRMNFY 145
           G+    F G+ N  L       Q F       +   +Q+ FA     + +G++  R  F 
Sbjct: 106 GESSDGFIGRHNFGL-------QNFPTLTSDYSFFLYQWAFAIAAAGITSGSIAERTKFV 165

Query: 146 AWMLFVPLWLTFSYTFVAFSIWSPNGF---------FFKMGLIDYSGGYVIHLSSGVAGF 205
           A++++      F Y  V+   WSP+G+          F  G ID++G  V+H+  G+AG 
Sbjct: 166 AYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSEDRLFGTGAIDFAGSGVVHMVGGIAGL 225

Query: 206 TAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD------PY--AA 265
             A   GPR+ +  +     +   ++  L++ G  LLW GW GFN G       PY   +
Sbjct: 226 WGALIEGPRIGRFPDGGHAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILIPYNSGS 285

Query: 266 NIDASLAVLNTHVCT----ATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAAGVV 325
           N      +  T V T     T+ LT L    +     +V     G++ G   IT    VV
Sbjct: 286 NYGQWSGIGRTAVTTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVV 345

Query: 326 QGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIFA-E 385
             WAAI+ G ++  +     ++   K A L K DD +     H   G+ G I  G+FA E
Sbjct: 346 DPWAAIVCGFVASLV-----LIGCNKLAELLKYDDPLEAAQLHGGCGAWGLIFVGLFAKE 405

Query: 386 PRLNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVLLQY 443
             +N V+  +  ++ GLF G        G + LG QL+ I+ +          +  +L+ 
Sbjct: 406 KYINEVYGASPGRHYGLFMGG-------GGKLLGAQLVQIIVIVGWVSATMGTLFFILKK 465

BLAST of Lsi08G011700 vs. TAIR 10
Match: AT3G24300.1 (ammonium transporter 1;3 )

HSP 1 Score: 112.5 bits (280), Expect = 9.6e-25
Identity = 123/448 (27.46%), Postives = 196/448 (43.75%), Query Frame = 0

Query: 26  DNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 85
           DN + L +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 86  GDKLAPFWGKLNVALDQEFLLEQAFGGKFPSATMVFF--QFVFAAITLVLIAGALLGRMN 145
           G     F G+ N AL ++F          P+A   FF  Q+ FA     + +G++  R  
Sbjct: 106 GGSSEGFIGRHNFAL-RDF--------PTPTADYSFFLYQWAFAIAAAGITSGSIAERTQ 165

Query: 146 FYAWMLFVPLWLTFSYTFVAFSIWSPNGF----------FFKMGLIDYSGGYVIHLSSGV 205
           F A++++      F Y  V+   WSP+G+           F  G ID++G  V+H+  G+
Sbjct: 166 FVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTGAIDFAGSGVVHMVGGI 225

Query: 206 AGFTAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD------PYA 265
           AG   A   GPR  +  +     +   ++  L++ G  LLW GW GFN G       PY 
Sbjct: 226 AGLWGALIEGPRRGRFEKGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILVPYN 285

Query: 266 ANID------ASLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCITPAA 325
           +  +           +NT +   T+ LT L    +     +V     G++ G   IT   
Sbjct: 286 SGSNYGQWSGIGRTAVNTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGC 345

Query: 326 GVVQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILSGIF 385
            VV+ WAAI+ G ++       +++   K A L + DD +     H   G+ G I  G+F
Sbjct: 346 SVVEPWAAIVCGFMAS-----VVLIGCNKLAELVQYDDPLEAAQLHGGCGAWGLIFVGLF 405

Query: 386 A-EPRLNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVTLINVIVTSLICVL 443
           A E  LN V+     +  GLF G        G + LG QL+ I+ +          +  +
Sbjct: 406 AKEKYLNEVYGATPGRPYGLFMGG-------GGKLLGAQLVQILVIVGWVSATMGTLFFI 465

BLAST of Lsi08G011700 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 101.7 bits (252), Expect = 1.7e-21
Identity = 120/458 (26.20%), Postives = 195/458 (42.58%), Query Frame = 0

Query: 26  DNAWQLTAATLVGLQSVPGLIILYGGAVKKKWAINSAFMALYAFACVLVCWVGWGYRLSF 85
           DN + L +A LV    + G  +L  G+V+ K  +N     +   A   + +  +GY  +F
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 86  GDKLAPFWGKLNVALDQEFLLEQAFGGK-FPSATMVF----FQFVFAAITLVLIAGALLG 145
           G     F GK              FG K  P+A+  +    +Q+ FA     + +G++  
Sbjct: 102 GSPSNGFIGK------------HYFGLKDIPTASADYSNFLYQWAFAIAAAGITSGSIAE 161

Query: 146 RMNFYAWMLFVPLWLTFSYTFVAFSIWSPNGF----------FFKMGLIDYSGGYVIHLS 205
           R  F A++++      F Y  V+   WS +G+           F  G ID++G  V+H+ 
Sbjct: 162 RTQFVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLFSTGAIDFAGSGVVHMV 221

Query: 206 SGVAGFTAAYWVGPRLSKDRE-----SFPPNNILLMLAGAGLLWMGWTGFNGGD------ 265
            G+AG   A   GPRL +        +   ++  L++ G  LLW GW GFN G       
Sbjct: 222 GGIAGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFNKILV 281

Query: 266 -----PYAANIDA-SLAVLNTHVCTATSLLTWLIFDIVFFRKPSVIGAVQGMITGLVCIT 325
                 Y     A     + T +   T+ LT L    +     +V     G++ G   IT
Sbjct: 282 TYETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAIT 341

Query: 326 PAAGVVQGWAAIIMGVLSGSIPWFTMMVVHKKSAILQKVDDTMAVLHTHAIAGSLGGILS 385
               VV+ WAAII G ++  +     ++   K A   K DD +     H   G+ G I +
Sbjct: 342 GGCSVVEPWAAIICGFVAALV-----LLGCNKLAEKLKYDDPLEAAQLHGGCGAWGLIFT 401

Query: 386 GIFAEPR-LNFVFYGAYNKYIGLFYGFNTKQVKLGFRQLGVQLLGIVFVT-LINVIVTSL 445
            +FA+ + LN ++     +  GLF G        G + LG QL+ I+ +T  ++  + +L
Sbjct: 402 ALFAQEKYLNQIYGNKPGRPHGLFMGG-------GGKLLGAQLIQIIVITGWVSATMGTL 461

Query: 446 ICVLLQYIVPLRMSEEDMAIGDEAAHGEEAYAIWGQGE 450
             +L +  +    SE++MA  D   HG  AY  +   E
Sbjct: 462 FFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDE 474

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A072VHJ15.2e-21776.31Ammonium transporter 2 member 5 OS=Medicago truncatula OX=3880 GN=AMT2-5 PE=2 SV... [more]
G7L1W71.1e-17165.78Ammonium transporter 2 member 4 OS=Medicago truncatula OX=3880 GN=AMT2-4 PE=2 SV... [more]
Q84KJ63.3e-17165.87Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
G7LAA89.9e-16863.14Ammonium transporter 2 member 3 OS=Medicago truncatula OX=3880 GN=AMT2-3 PE=2 SV... [more]
Q69T295.4e-16661.06Ammonium transporter 3 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3... [more]
Match NameE-valueIdentityDescription
A0A6J1HD346.5e-26395.77Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111462995 PE=3 SV=1[more]
A0A6J1K7F62.5e-26295.56Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111491842 PE=3 SV=1[more]
A0A1S3BCV93.8e-25593.07Ammonium transporter OS=Cucumis melo OX=3656 GN=LOC103488277 PE=3 SV=1[more]
A0A6J1BQ834.8e-25091.75Ammonium transporter OS=Momordica charantia OX=3673 GN=LOC111004759 PE=3 SV=1[more]
A0A6J5TRS84.1e-22581.18Ammonium transporter OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS9100 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022962592.11.3e-26295.77ammonium transporter 3 member 1-like [Cucurbita moschata] >KAG6598591.1 Ammonium... [more]
XP_023546976.15.1e-26295.56ammonium transporter 3 member 1-like [Cucurbita pepo subsp. pepo][more]
XP_022996665.15.1e-26295.56ammonium transporter 3 member 1-like [Cucurbita maxima][more]
XP_038885846.12.1e-26094.74ammonium transporter 2 member 5-like [Benincasa hispida][more]
XP_004138867.19.3e-25693.68ammonium transporter 3 member 1 [Cucumis sativus] >KAE8652192.1 hypothetical pro... [more]
Match NameE-valueIdentityDescription
AT2G38290.14.3e-15862.47ammonium transporter 2 [more]
AT2G38290.21.5e-11862.35ammonium transporter 2 [more]
AT3G24290.11.5e-2527.42ammonium transporter 1;5 [more]
AT3G24300.19.6e-2527.46ammonium transporter 1;3 [more]
AT4G13510.11.7e-2126.20ammonium transporter 1;1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002229Blood group Rhesus C/E/D polypeptidePRINTSPR00342RHESUSRHDcoord: 219..237
score: 41.52
coord: 250..272
score: 27.54
coord: 123..139
score: 25.49
IPR029020Ammonium/urea transporterGENE3D1.10.3430.10Ammonium transporter AmtB like domainscoord: 13..446
e-value: 3.5E-123
score: 413.6
IPR024041Ammonium transporter AmtB-like domainPFAMPF00909Ammonium_transpcoord: 28..442
e-value: 2.3E-83
score: 280.1
IPR001905Ammonium transporterTIGRFAMTIGR00836TIGR00836coord: 26..442
e-value: 2.5E-112
score: 373.7
NoneNo IPR availablePANTHERPTHR43029AMMONIUM TRANSPORTER MEP2coord: 6..474
NoneNo IPR availablePANTHERPTHR43029:SF7AMMONIUM TRANSPORTERcoord: 6..474
NoneNo IPR availableSUPERFAMILY111352Ammonium transportercoord: 21..424
IPR018047Ammonium transporter, conserved sitePROSITEPS01219AMMONIUM_TRANSPcoord: 180..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G011700.1Lsi08G011700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072488 ammonium transmembrane transport
biological_process GO:0019740 nitrogen utilization
biological_process GO:0015696 ammonium transport
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008519 ammonium transmembrane transporter activity