Lsi08G011650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G011650
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	2,6-dihydroxypyridine 3-monooxygenase-like
Location	chr08: 20287549 .. 20288061 (-)
RNA-Seq Expression	Lsi08G011650
Synteny	Lsi08G011650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGATGAAAGTAAATGAGGAGATGGTGAGAAAATTGCACCAACAAGTAGACGACTTTTGGGCCCCTGAGCTTGCAAAAGTCATTAGAGAAACAAAAAGTCCTTTCATCAATGTCATCTACGATTGTGATCCCTCAAAACAACTAGTTTGGGACAATGTGGTATTAGTTGGAGAAGCAGCTCATCCAACGACTCCTCATTGTGCAAGAAGCACAAATATGACGTTTTTAGATGCATCTGTTTTAGGTGAATGCCTCCTAAAATGGGGACCACTTGACATAAAATCAGCCCTTGCTGAGTATCAGTCTCTCCGGTTACCTGTCATTTATGCCCAAATCCTGCATTCTCGATACGTCGGTCGAATAAAACAAGGTTTGACACTTCCTGGCCGCGAATCCTTCGACCCAAATATAGCTACTACTACACAGAACTTACAAGAGCTTCAACTTAGAAATACACCTTTCCTTGATGATGTTGAGGATGGTGTTCCTCAAGTGATCGATCTAATTTAA

mRNA sequence

ATGACGATGAAAGTAAATGAGGAGATGGTGAGAAAATTGCACCAACAAGTAGACGACTTTTGGGCCCCTGAGCTTGCAAAAGTCATTAGAGAAACAAAAAGTCCTTTCATCAATGTCATCTACGATTGTGATCCCTCAAAACAACTAGTTTGGGACAATGTGGTATTAGTTGGAGAAGCAGCTCATCCAACGACTCCTCATTGTGCAAGAAGCACAAATATGACGTTTTTAGATGCATCTGTTTTAGGTGAATGCCTCCTAAAATGGGGACCACTTGACATAAAATCAGCCCTTGCTGAGTATCAGTCTCTCCGGTTACCTGTCATTTATGCCCAAATCCTGCATTCTCGATACGTCGGTCGAATAAAACAAGGTTTGACACTTCCTGGCCGCGAATCCTTCGACCCAAATATAGCTACTACTACACAGAACTTACAAGAGCTTCAACTTAGAAATACACCTTTCCTTGATGATGTTGAGGATGGTGTTCCTCAAGTGATCGATCTAATTTAA

Coding sequence (CDS)

ATGACGATGAAAGTAAATGAGGAGATGGTGAGAAAATTGCACCAACAAGTAGACGACTTTTGGGCCCCTGAGCTTGCAAAAGTCATTAGAGAAACAAAAAGTCCTTTCATCAATGTCATCTACGATTGTGATCCCTCAAAACAACTAGTTTGGGACAATGTGGTATTAGTTGGAGAAGCAGCTCATCCAACGACTCCTCATTGTGCAAGAAGCACAAATATGACGTTTTTAGATGCATCTGTTTTAGGTGAATGCCTCCTAAAATGGGGACCACTTGACATAAAATCAGCCCTTGCTGAGTATCAGTCTCTCCGGTTACCTGTCATTTATGCCCAAATCCTGCATTCTCGATACGTCGGTCGAATAAAACAAGGTTTGACACTTCCTGGCCGCGAATCCTTCGACCCAAATATAGCTACTACTACACAGAACTTACAAGAGCTTCAACTTAGAAATACACCTTTCCTTGATGATGTTGAGGATGGTGTTCCTCAAGTGATCGATCTAATTTAA

Protein sequence

MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEAAHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVGRIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGVPQVIDLI

Homology

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BLAST of Lsi08G011650 vs. ExPASy Swiss-Prot
Match: H1ZZA4 (Aurachin C monooxygenase/isomerase OS=Stigmatella aurantiaca OX=41 GN=auaG PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 3.5e-06
Identity = 35/106 (33.02%), Postives = 53/106 (50.00%), Query Frame = 0


Query: 21  WAPELAKVIRETKSPFI--NVIYDCDPSKQLVWDNVVLVGEAAHPTTPHCARSTNMTFLD 80
           W   + ++I  T S  I    I+D  P +Q V    VL+G+AAHP TP+  +       D
Sbjct: 245 WHAPIPQLIENTPSSAIMRTDIHDRVPIRQWVQGRAVLLGDAAHPMTPNMGQGGCQAVED 304

Query: 81  ASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVGRIKQ 125
           A VL  CL      ++ +ALA YQ++R+      +  S  +G+I Q
Sbjct: 305 AVVLARCLSL--EAELPAALARYQAVRVERANDFVAGSYRIGQIGQ 348

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BLAST of Lsi08G011650 vs. ExPASy Swiss-Prot
Match: Q0QFQ1 (3-hydroxybenzoate 6-hydroxylase 2 OS=Pseudomonas alcaligenes OX=43263 GN=hbzD PE=2 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 8.6e-05
Identity = 30/81 (37.04%), Postives = 40/81 (49.38%), Query Frame = 0


Query: 42  DCDPSKQLVWDNVVLVGEAAHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEY 101
           D +P  Q  +    L+G+AAHPTT + A+   M   DA  LGE L   G  D   AL  Y
Sbjct: 198 DREPIPQWTFGRTTLLGDAAHPTTQYMAQGACMALEDAVTLGEALRVHGN-DWGKALDLY 257

Query: 102 QSLRLPVIYAQILHSRYVGRI 123
           Q  R+      +L  R +GR+
Sbjct: 258 QRSRITRTARIVLSGREMGRL 277

[Close]

BLAST of Lsi08G011650 vs. ExPASy Swiss-Prot
Match: Q5EXK1 (3-hydroxybenzoate 6-hydroxylase OS=Klebsiella oxytoca OX=571 GN=mhbM PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-04
Identity = 27/81 (33.33%), Postives = 43/81 (53.09%), Query Frame = 0


Query: 42  DCDPSKQLVWDNVVLVGEAAHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEY 101
           D +P ++   D + LVG+AAHP   + A+   M   DA  LG+ L +    D   A A Y
Sbjct: 276 DREPVEKWGNDRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCDG-DAARAFALY 335

Query: 102 QSLRLPVIYAQILHSRYVGRI 123
           +S+R+P     +  +R +GR+
Sbjct: 336 ESVRIPRTARIVWSTREMGRV 355

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BLAST of Lsi08G011650 vs. ExPASy Swiss-Prot
Match: Q3S4B7 (3-hydroxybenzoate 6-hydroxylase OS=Polaromonas naphthalenivorans (strain CJ2) OX=365044 GN=nagX PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-04
Identity = 30/81 (37.04%), Postives = 40/81 (49.38%), Query Frame = 0


Query: 42  DCDPSKQLVWDNVVLVGEAAHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEY 101
           D +P  Q  +  V L+G+AAHPTT + A+   M   D   LGE  L+    D   A   Y
Sbjct: 279 DREPIGQWSFGRVTLLGDAAHPTTQYMAQGACMAMEDGVTLGEA-LRVNNNDFPKAFELY 338

Query: 102 QSLRLPVIYAQILHSRYVGRI 123
           Q  R+      +L SR +GRI
Sbjct: 339 QRSRVARTARIVLSSREMGRI 358

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BLAST of Lsi08G011650 vs. ExPASy Swiss-Prot
Match: Q88FY2 (6-hydroxynicotinate 3-monooxygenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) OX=160488 GN=nicC PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-04
Identity = 27/83 (32.53%), Postives = 44/83 (53.01%), Query Frame = 0


Query: 23  PELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEAAHPTTPHCARSTNMTFLDASVL 82
           P + K+I  T+S     + + +P        +VL+G+A HP  PH A+   M   DA++L
Sbjct: 258 PTVQKLIDATESITKWPLRNRNPLPLWSRGRLVLLGDACHPMKPHMAQGACMAIEDAAML 317

Query: 83  GECLLKWGPLDIKSALAEYQSLR 106
             CL + G  D ++A A Y++ R
Sbjct: 318 TRCLQETGLSDHRTAFALYEANR 340

[Close]

BLAST of Lsi08G011650 vs. ExPASy TrEMBL
Match: A0A6J1BPL9 (uncharacterized protein LOC111004675 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004675 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.1e-66
Identity = 123/170 (72.35%), Postives = 143/170 (84.12%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMK NEEMVRKLH+QVD+ W PE A+V++ETK PFINVIYD DP KQLVWDNVVLVGEA
Sbjct: 265 MTMKANEEMVRKLHKQVDEIWVPEFARVVKETKHPFINVIYDSDPLKQLVWDNVVLVGEA 324

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHPTTPHCARSTNM+ LDA VLG+CL KWG   +++ALAEYQSLRLP++  Q+LHSR+VG
Sbjct: 325 AHPTTPHCARSTNMSILDAEVLGKCLKKWGVEYLETALAEYQSLRLPIVSGQVLHSRHVG 384

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGVPQVIDLI 171
            IKQGL LP  E FD  + TTT+NLQE+Q+RNTPFLDD    VPQVI+L+
Sbjct: 385 LIKQGLALPNHERFDSMVVTTTENLQEIQIRNTPFLDD----VPQVINLV 430

[Close]

BLAST of Lsi08G011650 vs. ExPASy TrEMBL
Match: A0A6J1BR49 (uncharacterized protein LOC111004675 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111004675 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.1e-66
Identity = 123/170 (72.35%), Postives = 143/170 (84.12%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMK NEEMVRKLH+QVD+ W PE A+V++ETK PFINVIYD DP KQLVWDNVVLVGEA
Sbjct: 260 MTMKANEEMVRKLHKQVDEIWVPEFARVVKETKHPFINVIYDSDPLKQLVWDNVVLVGEA 319

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHPTTPHCARSTNM+ LDA VLG+CL KWG   +++ALAEYQSLRLP++  Q+LHSR+VG
Sbjct: 320 AHPTTPHCARSTNMSILDAEVLGKCLKKWGVEYLETALAEYQSLRLPIVSGQVLHSRHVG 379

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGVPQVIDLI 171
            IKQGL LP  E FD  + TTT+NLQE+Q+RNTPFLDD    VPQVI+L+
Sbjct: 380 LIKQGLALPNHERFDSMVVTTTENLQEIQIRNTPFLDD----VPQVINLV 425

[Close]

BLAST of Lsi08G011650 vs. ExPASy TrEMBL
Match: A0A6J1GIU3 (uncharacterized protein LOC111454588 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454588 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.2e-65
Identity = 119/163 (73.01%), Postives = 139/163 (85.28%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMKVNEEMVR+LH+Q ++ W PE A V+RETK PFIN IYDCDP +QLVWDNVVLVGEA
Sbjct: 259 MTMKVNEEMVRRLHEQANEIWVPEFANVVRETKDPFINAIYDCDPLEQLVWDNVVLVGEA 318

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHP TPHC RSTNM+ LDA+VLG+CL KWG  D+ SALAEYQSLRLP+I  Q+LHSR+VG
Sbjct: 319 AHPITPHCGRSTNMSILDAAVLGKCLQKWGAEDLNSALAEYQSLRLPIISEQVLHSRHVG 378

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGV 164
           RIKQGL LP R++FDPNIA   +NLQELQ+RN+PF DDV +G+
Sbjct: 379 RIKQGLALPNRQAFDPNIA--EENLQELQIRNSPFCDDVPEGI 419

[Close]

BLAST of Lsi08G011650 vs. ExPASy TrEMBL
Match: A0A6J1KNQ1 (uncharacterized protein LOC111496224 OS=Cucurbita maxima OX=3661 GN=LOC111496224 PE=4 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.7e-65
Identity = 118/163 (72.39%), Postives = 136/163 (83.44%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMKVNEEM R+LH+Q  + W PE A V+RETK PFIN IYDCDP +QLVWDNVVLVGE+
Sbjct: 259 MTMKVNEEMARRLHEQASEIWVPEFANVVRETKDPFINAIYDCDPLEQLVWDNVVLVGES 318

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHP TPHC RSTNM+ LDA+VLG+CL KWG  D+ SALAEYQSLRLP+I  Q+LHSR+VG
Sbjct: 319 AHPITPHCGRSTNMSILDAAVLGKCLQKWGAEDLNSALAEYQSLRLPIISEQVLHSRHVG 378

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGV 164
           RIKQGL LP R+ FDPNIA   +NLQELQ+RNTPF DDV +G+
Sbjct: 379 RIKQGLALPNRQVFDPNIA--EENLQELQIRNTPFCDDVPEGI 419

[Close]

BLAST of Lsi08G011650 vs. ExPASy TrEMBL
Match: A0A6J1GIN1 (uncharacterized protein LOC111454588 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454588 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.0e-64
Identity = 118/163 (72.39%), Postives = 138/163 (84.66%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMKVNEEMVR+LH+Q ++ W PE A V+RETK PFIN IYDCDP +QLVWDNVVLVGEA
Sbjct: 259 MTMKVNEEMVRRLHEQANEIWVPEFANVVRETKDPFINAIYDCDPLEQLVWDNVVLVGEA 318

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHP TPHC RSTNM+ LDA+VLG+CL KWG  D+ SALAEYQSLRLP+I  Q+LHSR+VG
Sbjct: 319 AHPITPHCGRSTNMSILDAAVLGKCLQKWGAEDLNSALAEYQSLRLPIISEQVLHSRHVG 378

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGV 164
           RIKQGL L  R++FDPN+A   +NLQELQ+RNTPF DDV +G+
Sbjct: 379 RIKQGLPLLNRQAFDPNVA--EENLQELQIRNTPFCDDVPEGI 419

[Close]

BLAST of Lsi08G011650 vs. NCBI nr
Match: XP_038886774.1 (aurachin C monooxygenase/isomerase-like [Benincasa hispida])

HSP 1 Score: 284.6 bits (727), Expect = 5.4e-73
Identity = 137/170 (80.59%), Postives = 150/170 (88.24%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           M+MKVNE MV+KLHQQVD+ W PELAKV++ETK PFINVIYDCDP +Q+VWDNVVLVGEA
Sbjct: 267 MSMKVNETMVKKLHQQVDEIWVPELAKVVKETKDPFINVIYDCDPLEQIVWDNVVLVGEA 326

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHPTTPHCARSTNM  LDA VLGECLLKWG LD+KSALAEYQSLRLPVIYAQ+LHSR+VG
Sbjct: 327 AHPTTPHCARSTNMALLDAFVLGECLLKWGLLDLKSALAEYQSLRLPVIYAQVLHSRHVG 386

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGVPQVIDLI 171
           RIKQG TL  RE FDP+ ATTTQN  ELQ+RNTPFLDD    VP+VIDLI
Sbjct: 387 RIKQGSTLSDRERFDPSAATTTQNFPELQIRNTPFLDD----VPRVIDLI 432

[Close]

BLAST of Lsi08G011650 vs. NCBI nr
Match: XP_038884093.1 (6-hydroxynicotinate 3-monooxygenase-like [Benincasa hispida])

HSP 1 Score: 268.1 bits (684), Expect = 5.3e-68
Identity = 127/169 (75.15%), Postives = 142/169 (84.02%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMKVNEE++RKLH+QVD+ W PE AKV+RETK PFIN IYDCDP +QLVWDNVVLVGEA
Sbjct: 259 MTMKVNEEILRKLHEQVDEIWVPEFAKVVRETKDPFINAIYDCDPLEQLVWDNVVLVGEA 318

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHPTTPHCARSTNM  LDA+VLG+CL KWG   +KSAL EYQSLRLPV+ AQ+LHSR  G
Sbjct: 319 AHPTTPHCARSTNMAILDAAVLGKCLQKWGAQGLKSALIEYQSLRLPVVSAQVLHSRRAG 378

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGVPQVIDL 170
           RIKQGLTLP  E FDPN+AT+ QN  ELQ+RN PF+DD    VPQVIDL
Sbjct: 379 RIKQGLTLPDCEPFDPNVATSIQNFPELQIRNIPFIDD----VPQVIDL 423

[Close]

BLAST of Lsi08G011650 vs. NCBI nr
Match: XP_022131482.1 (uncharacterized protein LOC111004675 isoform X2 [Momordica charantia])

HSP 1 Score: 261.2 bits (666), Expect = 6.4e-66
Identity = 123/170 (72.35%), Postives = 143/170 (84.12%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMK NEEMVRKLH+QVD+ W PE A+V++ETK PFINVIYD DP KQLVWDNVVLVGEA
Sbjct: 260 MTMKANEEMVRKLHKQVDEIWVPEFARVVKETKHPFINVIYDSDPLKQLVWDNVVLVGEA 319

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHPTTPHCARSTNM+ LDA VLG+CL KWG   +++ALAEYQSLRLP++  Q+LHSR+VG
Sbjct: 320 AHPTTPHCARSTNMSILDAEVLGKCLKKWGVEYLETALAEYQSLRLPIVSGQVLHSRHVG 379

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGVPQVIDLI 171
            IKQGL LP  E FD  + TTT+NLQE+Q+RNTPFLDD    VPQVI+L+
Sbjct: 380 LIKQGLALPNHERFDSMVVTTTENLQEIQIRNTPFLDD----VPQVINLV 425

[Close]

BLAST of Lsi08G011650 vs. NCBI nr
Match: XP_022131481.1 (uncharacterized protein LOC111004675 isoform X1 [Momordica charantia])

HSP 1 Score: 261.2 bits (666), Expect = 6.4e-66
Identity = 123/170 (72.35%), Postives = 143/170 (84.12%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMK NEEMVRKLH+QVD+ W PE A+V++ETK PFINVIYD DP KQLVWDNVVLVGEA
Sbjct: 265 MTMKANEEMVRKLHKQVDEIWVPEFARVVKETKHPFINVIYDSDPLKQLVWDNVVLVGEA 324

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHPTTPHCARSTNM+ LDA VLG+CL KWG   +++ALAEYQSLRLP++  Q+LHSR+VG
Sbjct: 325 AHPTTPHCARSTNMSILDAEVLGKCLKKWGVEYLETALAEYQSLRLPIVSGQVLHSRHVG 384

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGVPQVIDLI 171
            IKQGL LP  E FD  + TTT+NLQE+Q+RNTPFLDD    VPQVI+L+
Sbjct: 385 LIKQGLALPNHERFDSMVVTTTENLQEIQIRNTPFLDD----VPQVINLV 430

[Close]

BLAST of Lsi08G011650 vs. NCBI nr
Match: XP_023538145.1 (uncharacterized protein LOC111799003 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 260.4 bits (664), Expect = 1.1e-65
Identity = 119/163 (73.01%), Postives = 139/163 (85.28%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           MTMKVN+EMV +LH+Q ++ W PE A V+RETK PFIN IYDCDP +QLVWDNVVLVGEA
Sbjct: 254 MTMKVNKEMVMRLHEQANEIWVPEFANVVRETKDPFINAIYDCDPLEQLVWDNVVLVGEA 313

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHP TPHC RSTNM+ LDA+VLG+CL KWG  D+ SALAEYQSLRLP+IY Q+LHSR+VG
Sbjct: 314 AHPITPHCGRSTNMSILDAAVLGKCLQKWGAEDLNSALAEYQSLRLPIIYEQVLHSRHVG 373

Query: 121 RIKQGLTLPGRESFDPNIATTTQNLQELQLRNTPFLDDVEDGV 164
           RIKQGL LP R++FDPNIA   +NLQELQ+RNTPF DDV +G+
Sbjct: 374 RIKQGLALPNRQAFDPNIA--EENLQELQIRNTPFCDDVPEGI 414

[Close]

BLAST of Lsi08G011650 vs. TAIR 10
Match: AT5G11330.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 166.8 bits (421), Expect = 1.5e-41
Identity = 73/130 (56.15%), Postives = 100/130 (76.92%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           +T+KV++EM+ K+HQ+ +  W PELA+++ ETK PF+NVIYD DP +++ W N+VLVG+A
Sbjct: 257 VTLKVSQEMINKMHQEAETIWIPELARLMSETKDPFLNVIYDSDPLERIFWGNIVLVGDA 316

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQSLRLPVIYAQILHSRYVG 120
           AHPTTPH  RSTNM+ LDA VLG+CL   GP ++   L EYQ +RLPV+  Q+L++R +G
Sbjct: 317 AHPTTPHGLRSTNMSILDAEVLGKCLENCGPENVSLGLEEYQRIRLPVVSEQVLYARRLG 376

Query: 121 RIKQGLTLPG 131
           RIKQGL   G
Sbjct: 377 RIKQGLDHDG 386

[Close]

BLAST of Lsi08G011650 vs. TAIR 10
Match: AT5G11330.2 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 131.3 bits (329), Expect = 7.2e-31
Identity = 57/103 (55.34%), Postives = 79/103 (76.70%), Query Frame = 0


Query: 1   MTMKVNEEMVRKLHQQVDDFWAPELAKVIRETKSPFINVIYDCDPSKQLVWDNVVLVGEA 60
           +T+KV++EM+ K+HQ+ +  W PELA+++ ETK PF+NVIYD DP +++ W N+VLVG+A
Sbjct: 257 VTLKVSQEMINKMHQEAETIWIPELARLMSETKDPFLNVIYDSDPLERIFWGNIVLVGDA 316

Query: 61  AHPTTPHCARSTNMTFLDASVLGECLLKWGPLDIKSALAEYQS 104
           AHPTTPH  RSTNM+ LDA VLG+CL   GP   ++A   YQ+
Sbjct: 317 AHPTTPHGLRSTNMSILDAEVLGKCLENCGP---ENAFGAYQA 356

The following BLAST results are available for this feature:

BLAST of Lsi08G011650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H1ZZA4	3.5e-06	33.02	Aurachin C monooxygenase/isomerase OS=Stigmatella aurantiaca OX=41 GN=auaG PE=1 ...	[more]
Q0QFQ1	8.6e-05	37.04	3-hydroxybenzoate 6-hydroxylase 2 OS=Pseudomonas alcaligenes OX=43263 GN=hbzD PE...	[more]
Q5EXK1	1.1e-04	33.33	3-hydroxybenzoate 6-hydroxylase OS=Klebsiella oxytoca OX=571 GN=mhbM PE=1 SV=1	[more]
Q3S4B7	1.1e-04	37.04	3-hydroxybenzoate 6-hydroxylase OS=Polaromonas naphthalenivorans (strain CJ2) OX...	[more]
Q88FY2	1.9e-04	32.53	6-hydroxynicotinate 3-monooxygenase OS=Pseudomonas putida (strain ATCC 47054 / D...	[more]

BLAST of Lsi08G011650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BPL9	3.1e-66	72.35	uncharacterized protein LOC111004675 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1BR49	3.1e-66	72.35	uncharacterized protein LOC111004675 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1GIU3	1.2e-65	73.01	uncharacterized protein LOC111454588 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1KNQ1	7.7e-65	72.39	uncharacterized protein LOC111496224 OS=Cucurbita maxima OX=3661 GN=LOC111496224...	[more]
A0A6J1GIN1	1.0e-64	72.39	uncharacterized protein LOC111454588 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Lsi08G011650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886774.1	5.4e-73	80.59	aurachin C monooxygenase/isomerase-like [Benincasa hispida]	[more]
XP_038884093.1	5.3e-68	75.15	6-hydroxynicotinate 3-monooxygenase-like [Benincasa hispida]	[more]
XP_022131482.1	6.4e-66	72.35	uncharacterized protein LOC111004675 isoform X2 [Momordica charantia]	[more]
XP_022131481.1	6.4e-66	72.35	uncharacterized protein LOC111004675 isoform X1 [Momordica charantia]	[more]
XP_023538145.1	1.1e-65	73.01	uncharacterized protein LOC111799003 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi08G011650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G11330.1	1.5e-41	56.15	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT5G11330.2	7.2e-31	55.34	FAD/NAD(P)-binding oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 2..127 e-value: 2.9E-21 score: 78.1
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 41..129
None	No IPR available	PANTHER	PTHR47469	MONOOXYGENASE-LIKE	coord: 1..159
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 4..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G011650.1	Lsi08G011650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0004497	monooxygenase activity