Lsi08G011270 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G011270
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCaM_binding domain-containing protein
Locationchr08: 19899327 .. 19900691 (-)
RNA-Seq ExpressionLsi08G011270
SyntenyLsi08G011270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACAACTAAAGCAAAAGAAAGCTGTGGTTTGGGGAAGGAAAGGAAGATCACAGCTCCAAGTTCTGTCACAATTCCTAATAATACTCCAACCAAAAGAACTCCAAAATCATCTCCTAGAGGCATTATTTCTTCCACTGATCCAAAACTTTCTTCTTTTTCTTCTTCTTCTTCTTCTCCTTCTTCCTCTTCTTCCTCTGCTCCTTCCCTTTCCATTCTCCCCGAAAAACACGTCCCGAATTATCTCAAATCGACAGCCAGCTCCCGCCATGACCATAACTTCAGGCCAATAACCAAGAGTAAGTCTGGCCCTGCACCAATACCTGAGGGCAAACCTAGTTTGAATAGAAGAAGATCTTTCGATAAACCTCCCCCGACCGCACCCCGTTTGGAGAAGCCGTTTCGATCGCCTGGACCAAGGAATCGGGCTGGTCATGTTCCGGTCCGGTCGTCCTCTTTTGGGGCTAAACCCTCCACCACCACCACCACCACCAACACAATTGTCAATTCTACAAAGCCTGGTTTATTGGAGAGATCATCTTCTAAAGTCTCTAAAGTTGGAGGGAAACCACAACAACCAATTCAGGCTTTGAAAAGTAGCACTAGTAATGTAAAGAAAAGCTTGAAAAGAGAACCTAGCAATGCTGCTTCTGCTTCTGCTTCTGCTTCTGTTTCAGCTTCTGCTTCAGCAGAAATGATTCCCAAGACTAAGAATGCTATTGAACATGTAGATCAAAGTCCTTCTTTCGTTCTCGGTGTTAACGAAGAAGATTTAAAAAAGATCGAGTGCGAACTCGATCCGTATCTTCCTGATCCAATGCCTGAATTGGACAAACAACTCAGAATTGATCAGGTGGAAAAGAATGAGACTGATGACACAGAGAACTTCAAAGTAGTTGTTACTAATAAAGAGGTGGAGGAAGAAACTCCAGAGGCAGCTCAGACTGAAATGTCGTTAATGAATCGAGAACAAAACGAAGCGGAAGGAGACGAGGAGAAAACAGAGAGAATTACAGTGGAAAAAGAGGAAGTTATTGCATCAGAGGAAAACACAAACAACAAGAATGATCAAGAGAAGAGTGAAACAGATCCTATTTTAGATGGGGAGAGTTACAAGGAAGAAAATGATCACAAGGCGACCGAGACAACGGAGAAAGAAGGCGAAGCGACCGACAAAGTGGCGGCGGCAGTGGCAGCAGCAAAACCGCAACAAGGAGGACCGGCGAGAAAAGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGTAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGATTATGAATCTTCATCTAAATGA

mRNA sequence

ATGGCAACAACTAAAGCAAAAGAAAGCTGTGGTTTGGGGAAGGAAAGGAAGATCACAGCTCCAAGTTCTGTCACAATTCCTAATAATACTCCAACCAAAAGAACTCCAAAATCATCTCCTAGAGGCATTATTTCTTCCACTGATCCAAAACTTTCTTCTTTTTCTTCTTCTTCTTCTTCTCCTTCTTCCTCTTCTTCCTCTGCTCCTTCCCTTTCCATTCTCCCCGAAAAACACGTCCCGAATTATCTCAAATCGACAGCCAGCTCCCGCCATGACCATAACTTCAGGCCAATAACCAAGAGTAAGTCTGGCCCTGCACCAATACCTGAGGGCAAACCTAGTTTGAATAGAAGAAGATCTTTCGATAAACCTCCCCCGACCGCACCCCGTTTGGAGAAGCCGTTTCGATCGCCTGGACCAAGGAATCGGGCTGGTCATGTTCCGGTCCGGTCGTCCTCTTTTGGGGCTAAACCCTCCACCACCACCACCACCACCAACACAATTGTCAATTCTACAAAGCCTGGTTTATTGGAGAGATCATCTTCTAAAGTCTCTAAAGTTGGAGGGAAACCACAACAACCAATTCAGGCTTTGAAAAGTAGCACTAGTAATGTAAAGAAAAGCTTGAAAAGAGAACCTAGCAATGCTGCTTCTGCTTCTGCTTCTGCTTCTGTTTCAGCTTCTGCTTCAGCAGAAATGATTCCCAAGACTAAGAATGCTATTGAACATGTAGATCAAAGTCCTTCTTTCGTTCTCGGTGTTAACGAAGAAGATTTAAAAAAGATCGAGTGCGAACTCGATCCGTATCTTCCTGATCCAATGCCTGAATTGGACAAACAACTCAGAATTGATCAGGTGGAAAAGAATGAGACTGATGACACAGAGAACTTCAAAGTAGTTGTTACTAATAAAGAGGTGGAGGAAGAAACTCCAGAGGCAGCTCAGACTGAAATGTCGTTAATGAATCGAGAACAAAACGAAGCGGAAGGAGACGAGGAGAAAACAGAGAGAATTACAGTGGAAAAAGAGGAAGTTATTGCATCAGAGGAAAACACAAACAACAAGAATGATCAAGAGAAGAGTGAAACAGATCCTATTTTAGATGGGGAGAGTTACAAGGAAGAAAATGATCACAAGGCGACCGAGACAACGGAGAAAGAAGGCGAAGCGACCGACAAAGTGGCGGCGGCAGTGGCAGCAGCAAAACCGCAACAAGGAGGACCGGCGAGAAAAGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGTAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGATTATGAATCTTCATCTAAATGA

Coding sequence (CDS)

ATGGCAACAACTAAAGCAAAAGAAAGCTGTGGTTTGGGGAAGGAAAGGAAGATCACAGCTCCAAGTTCTGTCACAATTCCTAATAATACTCCAACCAAAAGAACTCCAAAATCATCTCCTAGAGGCATTATTTCTTCCACTGATCCAAAACTTTCTTCTTTTTCTTCTTCTTCTTCTTCTCCTTCTTCCTCTTCTTCCTCTGCTCCTTCCCTTTCCATTCTCCCCGAAAAACACGTCCCGAATTATCTCAAATCGACAGCCAGCTCCCGCCATGACCATAACTTCAGGCCAATAACCAAGAGTAAGTCTGGCCCTGCACCAATACCTGAGGGCAAACCTAGTTTGAATAGAAGAAGATCTTTCGATAAACCTCCCCCGACCGCACCCCGTTTGGAGAAGCCGTTTCGATCGCCTGGACCAAGGAATCGGGCTGGTCATGTTCCGGTCCGGTCGTCCTCTTTTGGGGCTAAACCCTCCACCACCACCACCACCACCAACACAATTGTCAATTCTACAAAGCCTGGTTTATTGGAGAGATCATCTTCTAAAGTCTCTAAAGTTGGAGGGAAACCACAACAACCAATTCAGGCTTTGAAAAGTAGCACTAGTAATGTAAAGAAAAGCTTGAAAAGAGAACCTAGCAATGCTGCTTCTGCTTCTGCTTCTGCTTCTGTTTCAGCTTCTGCTTCAGCAGAAATGATTCCCAAGACTAAGAATGCTATTGAACATGTAGATCAAAGTCCTTCTTTCGTTCTCGGTGTTAACGAAGAAGATTTAAAAAAGATCGAGTGCGAACTCGATCCGTATCTTCCTGATCCAATGCCTGAATTGGACAAACAACTCAGAATTGATCAGGTGGAAAAGAATGAGACTGATGACACAGAGAACTTCAAAGTAGTTGTTACTAATAAAGAGGTGGAGGAAGAAACTCCAGAGGCAGCTCAGACTGAAATGTCGTTAATGAATCGAGAACAAAACGAAGCGGAAGGAGACGAGGAGAAAACAGAGAGAATTACAGTGGAAAAAGAGGAAGTTATTGCATCAGAGGAAAACACAAACAACAAGAATGATCAAGAGAAGAGTGAAACAGATCCTATTTTAGATGGGGAGAGTTACAAGGAAGAAAATGATCACAAGGCGACCGAGACAACGGAGAAAGAAGGCGAAGCGACCGACAAAGTGGCGGCGGCAGTGGCAGCAGCAAAACCGCAACAAGGAGGACCGGCGAGAAAAGAGTCTCCAGCAATGTACAATCATGTAATAGAGGAGACAGCAAGTAAGCTTTTGGAAAAGAGGAAGAACAAGGTAAGAGCATTGGCTGGGGCATTTCAGACAGTCATTGATTATGAATCTTCATCTAAATGA

Protein sequence

MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSSPSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEKNETDDTENFKVVVTNKEVEEETPEAAQTEMSLMNREQNEAEGDEEKTERITVEKEEVIASEENTNNKNDQEKSETDPILDGESYKEENDHKATETTEKEGEATDKVAAAVAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Homology
BLAST of Lsi08G011270 vs. ExPASy TrEMBL
Match: A0A0A0LS85 (CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000 PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.7e-173
Identity = 370/472 (78.39%), Postives = 402/472 (85.17%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSS 60
           MATT++KESCGLGKERKIT PSSVTIP+NT TKRTPKSSPRGIISSTDPK  SF S SS+
Sbjct: 1   MATTRSKESCGLGKERKITPPSSVTIPSNTSTKRTPKSSPRGIISSTDPKFPSF-SPSSA 60

Query: 61  PSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRS 120
           PSS SS+  SLSILPEKHVPNYLKSTASSR+DHNF+PI +SKSGPAPIPEG+PSLNRRRS
Sbjct: 61  PSSPSSAPSSLSILPEKHVPNYLKSTASSRNDHNFKPIARSKSGPAPIPEGRPSLNRRRS 120

Query: 121 FDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERS 180
           FDKPPPT PRL KPFRSPGPRNR  HVPVRSSSFGAKPSTTTT   TI +STKPG LERS
Sbjct: 121 FDKPPPTVPRLGKPFRSPGPRNRTPHVPVRSSSFGAKPSTTTT---TIGHSTKPGFLERS 180

Query: 181 SSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNA---ASASASASVSASASAEMIPKT 240
           SSK SKVGGKPQ PIQ+LK+S+S VKKSL+RE SNA   ASASASAS SASASAE++PK+
Sbjct: 181 SSKSSKVGGKPQPPIQSLKTSSSYVKKSLRRESSNAAASASASASASASASASAEIVPKS 240

Query: 241 KNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK-----NETD 300
           KNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+R+DQVEK      +  
Sbjct: 241 KNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQIRLDQVEKKAVLDKDET 300

Query: 301 DTENFKVVVTNKEVEE--ETPEAAQTEMSLMNREQNEAEGDEEKTE----RITVEKEEVI 360
           DTE  KVVVTNKEVEE  ETP   +TE+SL N+EQNE EG+E K E    RI VEKEEV+
Sbjct: 301 DTEILKVVVTNKEVEEQKETPRTVETEISLANQEQNEREGEEPKEEDNPKRIEVEKEEVV 360

Query: 361 ASEENTNNKNDQEKSETDPILDGESYKEENDH----KATETTEKEGEATDKVAAAVAAAK 420
           A EE+ NNKNDQEKSETD +LDGES KEE+D     KA ETTEKE E+T+K       AK
Sbjct: 361 ALEESINNKNDQEKSETDSVLDGESCKEESDQNSTPKAAETTEKEAESTEK------TAK 420

Query: 421 PQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
           P+QGGP RKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 PRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 462

BLAST of Lsi08G011270 vs. ExPASy TrEMBL
Match: A0A5A7VBK4 (Serine/arginine repetitive matrix protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003220 PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 2.3e-172
Identity = 369/468 (78.85%), Postives = 401/468 (85.68%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSS 60
           MATT++KES GLGKER+IT PSSVTIPNNTPTKRTPKSSPRG ISSTDPK+ SFSSSS S
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  PSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRS 120
           PSS+ +   SLSILPEK VPNYLKSTASSR+DHNF+PIT+SKSGPAPIPEGKPSLNRRRS
Sbjct: 61  PSSAPT---SLSILPEKPVPNYLKSTASSRNDHNFKPITRSKSGPAPIPEGKPSLNRRRS 120

Query: 121 FDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERS 180
           FDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFGAKPST+TT    I +STKPG LERS
Sbjct: 121 FDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTT---IIGHSTKPGFLERS 180

Query: 181 SSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKNA 240
           SSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN A   ASAS SASASAE++PKTKNA
Sbjct: 181 SSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSNVA---ASASASASASAEIVPKTKNA 240

Query: 241 IEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK-----NETDDTE 300
           +EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+RIDQVEK      +  DTE
Sbjct: 241 VEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQVRIDQVEKKAALDKDETDTE 300

Query: 301 NFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAEGDEEKTE----RITVEKEEVIASEE 360
             KVVVTNKEVEE ETP  A+TE+SL NREQNE E +E K E    RI VEKEEV+A EE
Sbjct: 301 ILKVVVTNKEVEEKETPGTAETEISLANREQNEGEEEEPKEEDKPKRIGVEKEEVLALEE 360

Query: 361 NTNNKNDQEKSETDPILDGESYKEENDH----KATETTEKEGEATDKVAAAVAAAKPQQG 420
           + NNKNDQEKSETD +LDGES KEEND     KA ETTEKE E+T+K       AKP+QG
Sbjct: 361 SINNKNDQEKSETDSVLDGESCKEENDQNSTPKAAETTEKEAESTEK------TAKPRQG 420

Query: 421 GPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
            P RKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 APGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 453

BLAST of Lsi08G011270 vs. ExPASy TrEMBL
Match: A0A1S3BBU4 (serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488229 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 1.9e-171
Identity = 367/468 (78.42%), Postives = 399/468 (85.26%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSS 60
           MATT++KES GLGKER+IT PSSVTIPNNTPTKRTPKSSPRG ISSTDPK+ SFSSSS S
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  PSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRS 120
           PSS+ +   SLSILPEK VPNYLKSTASSR+DHNF+PIT+SKSGPAPIPEGKPSLNRRRS
Sbjct: 61  PSSAPT---SLSILPEKPVPNYLKSTASSRNDHNFKPITRSKSGPAPIPEGKPSLNRRRS 120

Query: 121 FDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERS 180
           FDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFGAKPST+TT    I +STKPG LERS
Sbjct: 121 FDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTT---IIGHSTKPGFLERS 180

Query: 181 SSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKNA 240
           SSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN A     AS SASASAE++PKTKNA
Sbjct: 181 SSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSNVA-----ASASASASAEIVPKTKNA 240

Query: 241 IEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK-----NETDDTE 300
           +EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+RIDQVEK      +  DTE
Sbjct: 241 VEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQVRIDQVEKKAALDKDETDTE 300

Query: 301 NFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAEGDEEKTE----RITVEKEEVIASEE 360
             KVVVTNKEVEE ETP  A+TE+SL NREQNE E +E K E    RI VEKEEV+A EE
Sbjct: 301 ILKVVVTNKEVEEKETPGTAETEISLANREQNEGEEEEPKEEDKPKRIGVEKEEVLALEE 360

Query: 361 NTNNKNDQEKSETDPILDGESYKEENDH----KATETTEKEGEATDKVAAAVAAAKPQQG 420
           + NNKNDQEKSETD +LDGES KEEND     KA ETTEKE E+T+K       AKP+QG
Sbjct: 361 SINNKNDQEKSETDSVLDGESCKEENDQNSTPKAAETTEKEAESTEK------TAKPRQG 420

Query: 421 GPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
            P RKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 APGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 451

BLAST of Lsi08G011270 vs. ExPASy TrEMBL
Match: A0A6J1K599 (microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX=3661 GN=LOC111492415 PE=4 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.7e-143
Identity = 343/491 (69.86%), Postives = 375/491 (76.37%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPN-NTPTKRTPKSSPRGIISSTDPKLSSFSSSSS 60
           MAT++AKESCGLGKERKIT PSSVTIPN NT TKRTPKSSPRGI  STD KLSSF    S
Sbjct: 1   MATSRAKESCGLGKERKITTPSSVTIPNPNTSTKRTPKSSPRGI--STDQKLSSF---PS 60

Query: 61  SPSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRR 120
           SPS SSSS  SLSILP+KHVPNYLKSTASSRHDHNF+PI KS +GPAPIPEGKPSLNRRR
Sbjct: 61  SPSPSSSSTHSLSILPQKHVPNYLKSTASSRHDHNFKPIKKS-AGPAPIPEGKPSLNRRR 120

Query: 121 SFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLER 180
           SFDKPPP   RL KPFRSP PRNRA H PVRSSSFGAKPST      T+V+STK G LER
Sbjct: 121 SFDKPPPAMQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPST------TLVSSTKTGFLER 180

Query: 181 SSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKN 240
           S SK+ KVGGK QQ +Q  KSS SN++KSLKRE SNAASAS         SAEM+PKTKN
Sbjct: 181 SGSKIYKVGGKTQQSVQTFKSS-SNLRKSLKRESSNAASAS---------SAEMVPKTKN 240

Query: 241 AIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQL-RIDQVEK-----NETDD 300
           A+EHV QSP  VLGVNEEDLKKIECELD  LPDP PELDK+L   DQVEK         D
Sbjct: 241 AVEHVGQSPVLVLGVNEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLHHHQPIQD 300

Query: 301 TENFKVVVTNKEVEEETPEAAQTEMSLM------NREQNEAEG-----DEEKTERITVEK 360
           T+ FKVV +NK+V EETP AA+T+ +        N+EQNE EG     +EEK ERI VEK
Sbjct: 301 TKIFKVVGSNKKV-EETPGAAETDNAKSSIWEDENQEQNEGEGEEEAKEEEKPERIEVEK 360

Query: 361 EE---VIASEENTNNKNDQEKSETDPIL------------DGESYKEENDHKATETTEKE 420
           EE   V+ASEE+TNNKN++EKS TDPI             DG+S KEEN +    +TEKE
Sbjct: 361 EEVYGVVASEESTNNKNEEEKSHTDPIQEGVIVLEHKEAEDGQSSKEENHN----STEKE 420

Query: 421 GEATDKVAAAVA----AAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAF 455
            EAT+KVAAA A    AAKP+QGGP RKESPA+YNHVIEETASKLLEKRKNKVRALAGAF
Sbjct: 421 AEATEKVAAAAAAAAEAAKPRQGGPGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAF 464

BLAST of Lsi08G011270 vs. ExPASy TrEMBL
Match: A0A6J1HE16 (neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462632 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.6e-141
Identity = 344/493 (69.78%), Postives = 373/493 (75.66%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPN-NTPTKRTPKSSPRGIISSTDPKLSSFSSSSS 60
           MAT++AKESCGLGKERKITAPSSVTIPN NT TKRTPKSSPRGI  STD KLSSF SS S
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNLNTSTKRTPKSSPRGI--STDQKLSSFPSSPS 60

Query: 61  SPSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRR 120
             SSSSSS  SLSILP+KHVPNYLKSTASSRHDHNF+PI KS  GPAPIPEGKPSLNRRR
Sbjct: 61  --SSSSSSTHSLSILPQKHVPNYLKSTASSRHDHNFKPIKKS-GGPAPIPEGKPSLNRRR 120

Query: 121 SFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLER 180
           SFDKPPP   RL KPFRSP PRNRA H PVRSSSFGAKPST      +IV+STK G LER
Sbjct: 121 SFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPST------SIVSSTKTGFLER 180

Query: 181 SSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKN 240
           S SK+ KVGGK QQ +Q LKSS SNV+KSLKRE SNAASAS         SAEM+PKTKN
Sbjct: 181 SGSKIYKVGGKTQQSVQTLKSS-SNVRKSLKRESSNAASAS---------SAEMVPKTKN 240

Query: 241 AIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQL-RIDQVEK-----NETDD 300
           A+EHV QSP  VLG NEEDLKKIECELD  LPDP PELDK+L   DQVEK         +
Sbjct: 241 AVEHVGQSPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLHHHQPIQE 300

Query: 301 TENFKVVVTNKEVEEETPEAAQTEMSLM------NREQNEAEG-----DEEKTERITVEK 360
           T+ FKVV +NK+V EETP AA+T+ +        NREQNE EG     +EEK ERI VEK
Sbjct: 301 TKIFKVVGSNKKV-EETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKPERIEVEK 360

Query: 361 EE---VIASEENTNNKNDQEKSETDPIL------------DGESYKEENDHKATETTEKE 420
           EE   V+ASEE+TNNKN++EKS T PI             DG+S KEEN      +TEKE
Sbjct: 361 EEVFGVVASEESTNNKNEEEKSHTVPIQEGVIVLEHKEAEDGQSSKEENH----TSTEKE 420

Query: 421 GEATDKVAAA------VAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAG 455
            EAT+KVAAA        AAKP+QGGP RKESPA+YNHVIEETASKLLEKRKNKVRALAG
Sbjct: 421 TEATEKVAAAATSAAEAVAAKPRQGGPGRKESPAVYNHVIEETASKLLEKRKNKVRALAG 467

BLAST of Lsi08G011270 vs. NCBI nr
Match: XP_038886465.1 (uncharacterized protein CG45076 [Benincasa hispida])

HSP 1 Score: 663.7 bits (1711), Expect = 1.1e-186
Identity = 387/463 (83.59%), Postives = 412/463 (88.98%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSS 60
           MATTKAKE+ GLGKER+ITAPSSVTIP +TPTKRTPKSSPRG+ISSTDPKLS  SSS S 
Sbjct: 1   MATTKAKENSGLGKERRITAPSSVTIPTHTPTKRTPKSSPRGLISSTDPKLS--SSSPSP 60

Query: 61  PSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRS 120
            SSSSSS  SLS+LPEKH+PNYLKSTASSRHDHNF+P+T+SKSGPAPIPEGKPSLNRRRS
Sbjct: 61  SSSSSSSTLSLSVLPEKHIPNYLKSTASSRHDHNFKPVTRSKSGPAPIPEGKPSLNRRRS 120

Query: 121 FDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERS 180
           FDKPP TAPRL KPFRSPGPRNRA HVPVRSSSFGAKPSTT     TIVNSTKPG L+RS
Sbjct: 121 FDKPPSTAPRLGKPFRSPGPRNRAAHVPVRSSSFGAKPSTT-----TIVNSTKPGFLDRS 180

Query: 181 SSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNA---ASASASASVSASASAEMIPKT 240
           SSK+SKVGGKPQQPI +LKSS SNVKKSLKRE SNA   AS SASAS SASASAE++PKT
Sbjct: 181 SSKISKVGGKPQQPIHSLKSSNSNVKKSLKRESSNAAASASVSASASASASASAEIVPKT 240

Query: 241 KNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK-----NETD 300
           KNA+EHVD SPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK      +  
Sbjct: 241 KNAVEHVDHSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEKKAVLDKDEA 300

Query: 301 DTENFKVVVTNKEVEEETPEAAQTEMSLMNREQNEAEGDEEKTERITVEKEEVIASEE-N 360
           DTE FKVVV NKEV EE PEAA+TE+SL NRE++E EG+EEK ERI VEKEEV+ SEE N
Sbjct: 301 DTETFKVVVANKEV-EEIPEAAETEISLTNRERDEVEGEEEKPERIAVEKEEVVGSEEIN 360

Query: 361 TNNKNDQEKSETDPILDGESYKEENDHKATETTEKEGEATDKVAAAVAAAKPQQGGPARK 420
           TNNKNDQEKSETDP+LDGESYKEENDHKA ETTEKE EAT+K AAAV  AKP+QGGP RK
Sbjct: 361 TNNKNDQEKSETDPVLDGESYKEENDHKAAETTEKEAEATEKAAAAV-VAKPRQGGPGRK 420

Query: 421 ESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
           ESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 ESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 454

BLAST of Lsi08G011270 vs. NCBI nr
Match: XP_011649760.1 (neurofilament heavy polypeptide [Cucumis sativus] >KGN62866.1 hypothetical protein Csa_021828 [Cucumis sativus])

HSP 1 Score: 618.2 bits (1593), Expect = 5.5e-173
Identity = 370/472 (78.39%), Postives = 402/472 (85.17%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSS 60
           MATT++KESCGLGKERKIT PSSVTIP+NT TKRTPKSSPRGIISSTDPK  SF S SS+
Sbjct: 1   MATTRSKESCGLGKERKITPPSSVTIPSNTSTKRTPKSSPRGIISSTDPKFPSF-SPSSA 60

Query: 61  PSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRS 120
           PSS SS+  SLSILPEKHVPNYLKSTASSR+DHNF+PI +SKSGPAPIPEG+PSLNRRRS
Sbjct: 61  PSSPSSAPSSLSILPEKHVPNYLKSTASSRNDHNFKPIARSKSGPAPIPEGRPSLNRRRS 120

Query: 121 FDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERS 180
           FDKPPPT PRL KPFRSPGPRNR  HVPVRSSSFGAKPSTTTT   TI +STKPG LERS
Sbjct: 121 FDKPPPTVPRLGKPFRSPGPRNRTPHVPVRSSSFGAKPSTTTT---TIGHSTKPGFLERS 180

Query: 181 SSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNA---ASASASASVSASASAEMIPKT 240
           SSK SKVGGKPQ PIQ+LK+S+S VKKSL+RE SNA   ASASASAS SASASAE++PK+
Sbjct: 181 SSKSSKVGGKPQPPIQSLKTSSSYVKKSLRRESSNAAASASASASASASASASAEIVPKS 240

Query: 241 KNAIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK-----NETD 300
           KNA+EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+R+DQVEK      +  
Sbjct: 241 KNAVEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQIRLDQVEKKAVLDKDET 300

Query: 301 DTENFKVVVTNKEVEE--ETPEAAQTEMSLMNREQNEAEGDEEKTE----RITVEKEEVI 360
           DTE  KVVVTNKEVEE  ETP   +TE+SL N+EQNE EG+E K E    RI VEKEEV+
Sbjct: 301 DTEILKVVVTNKEVEEQKETPRTVETEISLANQEQNEREGEEPKEEDNPKRIEVEKEEVV 360

Query: 361 ASEENTNNKNDQEKSETDPILDGESYKEENDH----KATETTEKEGEATDKVAAAVAAAK 420
           A EE+ NNKNDQEKSETD +LDGES KEE+D     KA ETTEKE E+T+K       AK
Sbjct: 361 ALEESINNKNDQEKSETDSVLDGESCKEESDQNSTPKAAETTEKEAESTEK------TAK 420

Query: 421 PQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
           P+QGGP RKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 PRQGGPGRKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 462

BLAST of Lsi08G011270 vs. NCBI nr
Match: KAA0064998.1 (serine/arginine repetitive matrix protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 615.1 bits (1585), Expect = 4.7e-172
Identity = 369/468 (78.85%), Postives = 401/468 (85.68%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSS 60
           MATT++KES GLGKER+IT PSSVTIPNNTPTKRTPKSSPRG ISSTDPK+ SFSSSS S
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  PSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRS 120
           PSS+ +   SLSILPEK VPNYLKSTASSR+DHNF+PIT+SKSGPAPIPEGKPSLNRRRS
Sbjct: 61  PSSAPT---SLSILPEKPVPNYLKSTASSRNDHNFKPITRSKSGPAPIPEGKPSLNRRRS 120

Query: 121 FDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERS 180
           FDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFGAKPST+TT    I +STKPG LERS
Sbjct: 121 FDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTT---IIGHSTKPGFLERS 180

Query: 181 SSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKNA 240
           SSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN A   ASAS SASASAE++PKTKNA
Sbjct: 181 SSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSNVA---ASASASASASAEIVPKTKNA 240

Query: 241 IEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK-----NETDDTE 300
           +EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+RIDQVEK      +  DTE
Sbjct: 241 VEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQVRIDQVEKKAALDKDETDTE 300

Query: 301 NFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAEGDEEKTE----RITVEKEEVIASEE 360
             KVVVTNKEVEE ETP  A+TE+SL NREQNE E +E K E    RI VEKEEV+A EE
Sbjct: 301 ILKVVVTNKEVEEKETPGTAETEISLANREQNEGEEEEPKEEDKPKRIGVEKEEVLALEE 360

Query: 361 NTNNKNDQEKSETDPILDGESYKEENDH----KATETTEKEGEATDKVAAAVAAAKPQQG 420
           + NNKNDQEKSETD +LDGES KEEND     KA ETTEKE E+T+K       AKP+QG
Sbjct: 361 SINNKNDQEKSETDSVLDGESCKEENDQNSTPKAAETTEKEAESTEK------TAKPRQG 420

Query: 421 GPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
            P RKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 APGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 453

BLAST of Lsi08G011270 vs. NCBI nr
Match: XP_008445084.1 (PREDICTED: serine/arginine repetitive matrix protein 1 [Cucumis melo])

HSP 1 Score: 612.1 bits (1577), Expect = 4.0e-171
Identity = 367/468 (78.42%), Postives = 399/468 (85.26%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSS 60
           MATT++KES GLGKER+IT PSSVTIPNNTPTKRTPKSSPRG ISSTDPK+ SFSSSS S
Sbjct: 1   MATTRSKESGGLGKERRITPPSSVTIPNNTPTKRTPKSSPRGFISSTDPKVPSFSSSSPS 60

Query: 61  PSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRS 120
           PSS+ +   SLSILPEK VPNYLKSTASSR+DHNF+PIT+SKSGPAPIPEGKPSLNRRRS
Sbjct: 61  PSSAPT---SLSILPEKPVPNYLKSTASSRNDHNFKPITRSKSGPAPIPEGKPSLNRRRS 120

Query: 121 FDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERS 180
           FDKPPPTAPRLEKPFRSPGPRNRA HVPVRSSSFGAKPST+TT    I +STKPG LERS
Sbjct: 121 FDKPPPTAPRLEKPFRSPGPRNRATHVPVRSSSFGAKPSTSTT---IIGHSTKPGFLERS 180

Query: 181 SSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKNA 240
           SSK+SKVGGKPQ PIQ+LK+S+S+VKKSL+RE SN A     AS SASASAE++PKTKNA
Sbjct: 181 SSKISKVGGKPQPPIQSLKTSSSHVKKSLRRESSNVA-----ASASASASAEIVPKTKNA 240

Query: 241 IEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQLRIDQVEK-----NETDDTE 300
           +EHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQ+RIDQVEK      +  DTE
Sbjct: 241 VEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQVRIDQVEKKAALDKDETDTE 300

Query: 301 NFKVVVTNKEVEE-ETPEAAQTEMSLMNREQNEAEGDEEKTE----RITVEKEEVIASEE 360
             KVVVTNKEVEE ETP  A+TE+SL NREQNE E +E K E    RI VEKEEV+A EE
Sbjct: 301 ILKVVVTNKEVEEKETPGTAETEISLANREQNEGEEEEPKEEDKPKRIGVEKEEVLALEE 360

Query: 361 NTNNKNDQEKSETDPILDGESYKEENDH----KATETTEKEGEATDKVAAAVAAAKPQQG 420
           + NNKNDQEKSETD +LDGES KEEND     KA ETTEKE E+T+K       AKP+QG
Sbjct: 361 SINNKNDQEKSETDSVLDGESCKEENDQNSTPKAAETTEKEAESTEK------TAKPRQG 420

Query: 421 GPARKESPAMYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 455
            P RKESPA+YNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK
Sbjct: 421 APGRKESPAVYNHVIEETASKLLEKRKNKVRALAGAFQTVIDYESSSK 451

BLAST of Lsi08G011270 vs. NCBI nr
Match: XP_023546370.1 (cell wall protein RBR3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 521.5 bits (1342), Expect = 7.0e-144
Identity = 347/494 (70.24%), Postives = 376/494 (76.11%), Query Frame = 0

Query: 1   MATTKAKESCGLGKERKITAPSSVTIPN-NTPTKRTPKSSPRGIISSTDPKLSSFSSSSS 60
           MAT++AKESCGLGKERKITAPSSVTIPN NT TKRTPKSSPRGI  STD KLSSF SS S
Sbjct: 1   MATSRAKESCGLGKERKITAPSSVTIPNVNTSTKRTPKSSPRGI--STDQKLSSFPSSPS 60

Query: 61  SPSSSSSSAPSLSILPEKHVPNYLKSTASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRR 120
             SSSSSS  SLSILP+KHVPNYLKSTASSRHDHNF+PI KS +GP PIPEGKPSLNRRR
Sbjct: 61  --SSSSSSTHSLSILPQKHVPNYLKSTASSRHDHNFKPIKKS-AGPTPIPEGKPSLNRRR 120

Query: 121 SFDKPPPTAPRLEKPFRSPGPRNRAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLER 180
           SFDKPPP   RL KPFRSP PRNRA H PVRSSSFGAKPST      TIV+STK G LER
Sbjct: 121 SFDKPPPATQRLGKPFRSPEPRNRAAHAPVRSSSFGAKPST------TIVSSTKTGFLER 180

Query: 181 SSSKVSKVGGKPQQPIQALKSSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKN 240
           S SK+ KVGGK QQ +Q LKSS SNV+KSLKRE SNAASAS         SAEM+PKTKN
Sbjct: 181 SGSKIYKVGGKTQQSVQTLKSS-SNVRKSLKRESSNAASAS---------SAEMVPKTKN 240

Query: 241 AIEHVDQSPSFVLGVNEEDLKKIECELDPYLPDPMPELDKQL-RIDQVEK-----NETDD 300
           A+EHV QSP  VLG NEEDLKKIECELD  LPDP PELDK+L   DQVEK         D
Sbjct: 241 AVEHVGQSPVLVLGANEEDLKKIECELDLCLPDPRPELDKELSTTDQVEKRLHHHQPIQD 300

Query: 301 TENFKVVVTNKEVEEETPEAAQTEMSLM------NREQNEAEG-----DEEKTERITVEK 360
           T+ FKVV +NK+V EETP AA+T+ +        NREQNE EG     +EEK ERI VEK
Sbjct: 301 TKIFKVVGSNKKV-EETPGAAETDNAKSSIREDENREQNEGEGEEEAKEEEKPERIEVEK 360

Query: 361 EE---VIASEENTNNKNDQEKSETDPIL------------DGESYKEENDHKATETTEKE 420
           EE   V+ASEE+TNNKN++EKS TDPI             DG+S KEEN +    +TEKE
Sbjct: 361 EEVFGVVASEESTNNKNEEEKSHTDPIQVGVIVLEHKEVEDGQSSKEENHN----STEKE 420

Query: 421 GEATDKVAAA-------VAAAKPQQGGPARKESPAMYNHVIEETASKLLEKRKNKVRALA 455
            EAT+KVAAA        AAAKP+QGGP RKESPA+YNHVIEETASKLLEKRKNKVRALA
Sbjct: 421 AEATEKVAAAAAAAAAEAAAAKPRQGGPGRKESPAVYNHVIEETASKLLEKRKNKVRALA 468

BLAST of Lsi08G011270 vs. TAIR 10
Match: AT5G24880.1 (BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (TAIR:AT5G10660.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 55.8 bits (133), Expect = 1.0e-07
Identity = 137/473 (28.96%), Postives = 204/473 (43.13%), Query Frame = 0

Query: 32  TKRTPKSSPRGIISSTDPKLSSFSSSSSSP----SSSSSSAPSLSILPEKHVPNYLKSTA 91
           T  T K+ P       +  LS   S    P    S++SS   S    PEK +PNYLK T 
Sbjct: 4   TSSTTKAKP------DNQNLSRTKSLGRKPKQPLSTASSEDGSSDQKPEKPLPNYLKPTI 63

Query: 92  SSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRSFDKPPPTAPRLEKPFRSPGPRNRAGHV 151
           SSR D   + + K+ +      +    L RRRSFD+PP +   L  P  S  PR +    
Sbjct: 64  SSRPDAG-KLLKKNNNS----LDDNQKLLRRRSFDRPPSS---LTSPSTSASPRIQ---- 123

Query: 152 PVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERSSS-----KVSKVGGKPQQPIQALK--- 211
                S    PS   +     V   KP    RSSS      V K G   + P  A K   
Sbjct: 124 ----KSLNVSPS--RSRDRPAVPREKPVTALRSSSFHGSRNVPKGGNTAKSPPVAPKKSG 183

Query: 212 -SSTSNVKKSLKREPSNAASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEED 271
            +S+S   KS K    N     AS    A  SA M    ++  E + +  S  L V++ D
Sbjct: 184 LNSSSTSSKSKKEGSENVRIKKASDKEIALDSASMSSAQEDHQEEILKVESDHLQVSDHD 243

Query: 272 LKKIECELDPYLPDPMPELDKQLRIDQVEKNETDDTENF--------------------- 331
           +++ + E +        E + Q ++ Q  ++  +  E+                      
Sbjct: 244 IEEPKYEKE--------EKEVQEKVVQANESVEEKAESSGPTPVASPVGKDCNAVVAELE 303

Query: 332 -KVVVTNKEVEEETPEAAQTEMSLMNREQNEAE-----GDEEKTERITVEKEEVIASEEN 391
            K++    ++EE+T E  + + +  N+ + E +      + E  E++  E +EV + EE 
Sbjct: 304 EKLIKNEDDIEEKTEEMKEQDNNQANKSEEEEDVKKKIDENETPEKVDTESKEVESVEET 363

Query: 392 TNNKNDQEKSE-----TDPILDGESYKEENDHKATETTEKEGEATDKVAAAV-----AAA 451
           T  K ++ K E      +   + E  KE++  +  E  EKE    D+    V     A  
Sbjct: 364 TQEKEEEVKEEGKERVEEEEKEKEKVKEDDQKEKVEEEEKEKVKGDEEKEKVKEEESAEG 423

Query: 452 KPQQGGPARKESPAMYNHVIEETASKLLEK-RKNKVRALAGAFQTVIDYESSS 454
           K ++    +KESP+ YN VI   ASK+ E  RKNKV ALAGAFQTVIDYE+++
Sbjct: 424 KKKEVVKGKKESPSAYNDVI---ASKMQENPRKNKVLALAGAFQTVIDYETAA 441

BLAST of Lsi08G011270 vs. TAIR 10
Match: AT5G10660.1 (calmodulin-binding protein-related )

HSP 1 Score: 52.0 bits (123), Expect = 1.5e-06
Identity = 137/451 (30.38%), Postives = 198/451 (43.90%), Query Frame = 0

Query: 25  TIPNNTPTKRTPKSSPRGIISSTDPKLSSFSSSSSSPSSSSSSAPSLSILPEKHVPNYLK 84
           T  + T TKR  K+  R       PK      SSS P +++  +   +I   K +PNYLK
Sbjct: 3   TTSSTTKTKRDNKNLTRSKSLGRKPK----PVSSSEPEANADGSDRKTI--GKPLPNYLK 62

Query: 85  STASSRHDHNFRPITKSKSGPAPIPEGKPSLNRRRSFDKPPP--TAPRLEKPFRSPGPRN 144
            T SSR D    P+   +   A   E    L RRRSFD PP   T+P      RS     
Sbjct: 63  PTISSRPD----PVKFLRKNNA--VEENQKLLRRRSFDHPPSSLTSPSTSSAHRSLNTSP 122

Query: 145 RAGHVPVRSSSFGAKPSTTTTTTNTIVNSTKPGLLERSSSKVSKVGGKPQQPIQALKSST 204
              H+  + +    KP T   +T +   S++ GL  R SS V       + P  A + S+
Sbjct: 123 AHPHLRDKPAVPREKPVTGLRST-SFHGSSRGGL--RGSSTV-------KSPPVASRGSS 182

Query: 205 SNVKKSLKREPSNAASASASASVSASASAEMIPKTKNAIEHVDQSPSFVLGVNEEDLKKI 264
              K  L    S+ +    S +V   +S + I          D SP      +EE++ K+
Sbjct: 183 GVKKSGLSGNSSSKSKKEGSGNVPKKSSGKEISP--------DSSPLASAHEDEEEIVKV 242

Query: 265 ECELDPYLPDPMPELDKQLRIDQVEKNETDDTENFKVVVTNKEVEEETPEAAQTE--MSL 324
           E ++                 D  E+ + +D + F     + E +E +P AA TE     
Sbjct: 243 ETDVH--------------ISDHGEEPKEEDKDQFAQPDESGEEKETSPVAASTEEQKGE 302

Query: 325 MNREQNEAEGDEEKTERITVEKEEVIASEENTNNKNDQEKSET-------DPILDGESYK 384
           +  E    E  EE  E   +E+      EE     +D+E SET       +  ++ E  K
Sbjct: 303 LIDEDKSTEQIEEPKEPENIEENNSEEEEEVKKKSDDEENSETVATTTDMNEAVNVEESK 362

Query: 385 EENDHKATETTEKEGE---ATDKVAAAVAAAK--PQQGG-----PARKESPAMYNHVIEE 444
           EE   +A E  E+EGE   A ++    +A  +  P++G        +KESP  YN VI  
Sbjct: 363 EEEKEEA-EVKEEEGESSAAKEETTETMAQVEELPEEGTKNEVVQGKKESPTAYNDVI-- 405

Query: 445 TASKLLE-KRKNKVRALAGAFQTVIDYESSS 454
            ASK+ E  +KNKV ALAGAFQTVIDYE+++
Sbjct: 423 -ASKMQENSKKNKVLALAGAFQTVIDYETAA 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0LS852.7e-17378.39CaM_binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G378000... [more]
A0A5A7VBK42.3e-17278.85Serine/arginine repetitive matrix protein 1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BBU41.9e-17178.42serine/arginine repetitive matrix protein 1 OS=Cucumis melo OX=3656 GN=LOC103488... [more]
A0A6J1K5991.7e-14369.86microtubule-associated protein RP/EB family member 1-like OS=Cucurbita maxima OX... [more]
A0A6J1HE161.6e-14169.78neurofilament medium polypeptide-like OS=Cucurbita moschata OX=3662 GN=LOC111462... [more]
Match NameE-valueIdentityDescription
XP_038886465.11.1e-18683.59uncharacterized protein CG45076 [Benincasa hispida][more]
XP_011649760.15.5e-17378.39neurofilament heavy polypeptide [Cucumis sativus] >KGN62866.1 hypothetical prote... [more]
KAA0064998.14.7e-17278.85serine/arginine repetitive matrix protein 1 [Cucumis melo var. makuwa][more]
XP_008445084.14.0e-17178.42PREDICTED: serine/arginine repetitive matrix protein 1 [Cucumis melo][more]
XP_023546370.17.0e-14470.24cell wall protein RBR3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G24880.11.0e-0728.96BEST Arabidopsis thaliana protein match is: calmodulin-binding protein-related (... [more]
AT5G10660.11.5e-0630.38calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 316..448
e-value: 0.0089
score: 6.6
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 343..447
e-value: 1.7E-13
score: 51.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 324..391
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 301..391
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..228
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 21..72
NoneNo IPR availablePANTHERPTHR33349:SF20CALMODULIN-BINDING PROTEIN-LIKE PROTEINcoord: 1..451
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 1..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G011270.1Lsi08G011270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding