Lsi08G011080 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G011080
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	calmodulin-like protein 7
Location	chr08: 19729144 .. 19729677 (+)
RNA-Seq Expression	Lsi08G011080
Synteny	Lsi08G011080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTATCTCTAGATTCATTGGGGACTTTCTACAAACACTTGGTGGGCGGTCCCGAGCAACGGCGGAATCCGCCATTAACGACGGCGAATCAAGGCCCCACAGAATGGCATACGACGACGTATTACTCAGGGCGTTGATTACGGTGTTTGGGATGAGGGAAAACGGGAGGATCAAAAAGGAAAAAGCAAAGGGGGTGGTGGAGAAACTTGGGTTGATAGAGAGTGAAGAAGAGAAGGAGAAGTTCGAGTTGGCGGCGGCGGAGGACGGCGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGGAGGAAGGGACGAGAAATGAGCTGCTTTATGAAGCGTTTAAGATATTTGATGTGGATGGAGATGGGTTTATTGACACAGTGGAATTGAAAAGAGTGCTTGATTGTTTGGGATTAGATAAAGGATGGGGAATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTTAATTTGGATGGCAAAGTTGATTTTAGTGAGTTTGAATTAATGATGGGTGTTAAATGCCTTTGA

mRNA sequence

ATGGGTATCTCTAGATTCATTGGGGACTTTCTACAAACACTTGGTGGGCGGTCCCGAGCAACGGCGGAATCCGCCATTAACGACGGCGAATCAAGGCCCCACAGAATGGCATACGACGACGTATTACTCAGGGCGTTGATTACGGTGTTTGGGATGAGGGAAAACGGGAGGATCAAAAAGGAAAAAGCAAAGGGGGTGGTGGAGAAACTTGGGTTGATAGAGAGTGAAGAAGAGAAGGAGAAGTTCGAGTTGGCGGCGGCGGAGGACGGCGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGGAGGAAGGGACGAGAAATGAGCTGCTTTATGAAGCGTTTAAGATATTTGATGTGGATGGAGATGGGTTTATTGACACAGTGGAATTGAAAAGAGTGCTTGATTGTTTGGGATTAGATAAAGGATGGGGAATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTTAATTTGGATGGCAAAGTTGATTTTAGTGAGTTTGAATTAATGATGGGTGTTAAATGCCTTTGA

Coding sequence (CDS)

ATGGGTATCTCTAGATTCATTGGGGACTTTCTACAAACACTTGGTGGGCGGTCCCGAGCAACGGCGGAATCCGCCATTAACGACGGCGAATCAAGGCCCCACAGAATGGCATACGACGACGTATTACTCAGGGCGTTGATTACGGTGTTTGGGATGAGGGAAAACGGGAGGATCAAAAAGGAAAAAGCAAAGGGGGTGGTGGAGAAACTTGGGTTGATAGAGAGTGAAGAAGAGAAGGAGAAGTTCGAGTTGGCGGCGGCGGAGGACGGCGATGAGGTGGCGGTGGAGGAGGTGATCGGAGAGGAGGAAGGGACGAGAAATGAGCTGCTTTATGAAGCGTTTAAGATATTTGATGTGGATGGAGATGGGTTTATTGACACAGTGGAATTGAAAAGAGTGCTTGATTGTTTGGGATTAGATAAAGGATGGGGAATTAGGGAGATTGAGAAAATGGTTAGTGTTGTGGATGTTAATTTGGATGGCAAAGTTGATTTTAGTGAGTTTGAATTAATGATGGGTGTTAAATGCCTTTGA

Protein sequence

MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGEEEGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL

Homology

[Close]

BLAST of Lsi08G011080 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.6e-12
Identity = 37/70 (52.86%), Postives = 48/70 (68.57%), Query Frame = 0


Query: 103 EGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGK 162
           +G+    L EAF +FD DGDGFI  VEL++VL  LGL +   I ++EKM+  VD N DG+
Sbjct: 105 DGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGR 164

Query: 163 VDFSEFELMM 173
           VDF EF+ MM
Sbjct: 165 VDFFEFKNMM 174

[Close]

BLAST of Lsi08G011080 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-11
Identity = 46/136 (33.82%), Postives = 72/136 (52.94%), Query Frame = 0


Query: 43  LRALITVFGMRENGRIKKEKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGE- 102
           L  +  +F    +G+I K + K   + +G++  E E  +       +GD     +  G  
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 103 -----EEGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVD 162
                EE    E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 163 VNLDGKVDFSEFELMM 173
           V+ DG V+F EF+ MM
Sbjct: 126 VDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi08G011080 vs. ExPASy Swiss-Prot
Match: Q84UL5 (Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 GN=CML32 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 3.7e-11
Identity = 40/84 (47.62%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 89  DGDEVAVEEVIGEEEGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREI 148
           DG   A E   G   G   E + EAFK+FDVDGDGFI   EL+ VL  LGL +   +  +
Sbjct: 107 DGAAAAGEP--GGGGGDEEEEMREAFKVFDVDGDGFISASELQEVLKKLGLPEAGSLATV 166

Query: 149 EKMVSVVDVNLDGKVDFSEFELMM 173
            +M+  VD N DG+VDF EF+ MM
Sbjct: 167 REMICNVDRNSDGRVDFGEFKSMM 188

[Close]

BLAST of Lsi08G011080 vs. ExPASy Swiss-Prot
Match: Q7XHW4 (Probable calcium-binding protein CML24 OS=Oryza sativa subsp. japonica OX=39947 GN=CML24 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-11
Identity = 33/69 (47.83%), Postives = 46/69 (66.67%), Query Frame = 0


Query: 104 GTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKV 163
           G  +E + EAFK+FDVDGDGFI   EL+ VL  LG+ +   +  + +M+  VD + DG+V
Sbjct: 121 GGGDEEMKEAFKVFDVDGDGFISASELQEVLKKLGMPEAGSLANVREMICNVDRDSDGRV 180

Query: 164 DFSEFELMM 173
           DF EF+ MM
Sbjct: 181 DFGEFKCMM 189

[Close]

BLAST of Lsi08G011080 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.9e-11
Identity = 49/143 (34.27%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 39  DDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIESEEEKEKFELAAAEDGDE-VAVEE 98
           D   L  +  +F    +G+I  ++     + LG+I  E+E  +       +GD  V +EE
Sbjct: 2   DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEE 61

Query: 99  --------VIGEEEGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIE 158
                   ++ +E+    E + EAF +FD +GDGFI   ELK VL  LGL +G  + E  
Sbjct: 62  FGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 121

Query: 159 KMVSVVDVNLDGKVDFSEFELMM 173
           KM+  VDV+ DG+V++ EF  MM
Sbjct: 122 KMIMQVDVDGDGRVNYMEFRQMM 144

[Close]

BLAST of Lsi08G011080 vs. ExPASy TrEMBL
Match: A0A5A7VBM3 (Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003420 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 6.1e-81
Identity = 161/177 (90.96%), Postives = 166/177 (93.79%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGRSRA AES I+ GES+P+RM YDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGEEEGTRNELLYEAFKIFDVD 120
           EKAKGVVEKLGLI   EEKEKFELAA E GDEVAVEEVIGEEEG RNELLYEAFKIFDVD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDVD 120

Query: 121 GDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 178
           GDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 GDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

[Close]

BLAST of Lsi08G011080 vs. ExPASy TrEMBL
Match: A0A1S3BBS0 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 6.1e-81
Identity = 161/177 (90.96%), Postives = 166/177 (93.79%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGRSRA AES I+ GES+P+RM YDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGEEEGTRNELLYEAFKIFDVD 120
           EKAKGVVEKLGLI   EEKEKFELAA E GDEVAVEEVIGEEEG RNELLYEAFKIFDVD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDVD 120

Query: 121 GDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 178
           GDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 GDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

[Close]

BLAST of Lsi08G011080 vs. ExPASy TrEMBL
Match: A0A0A0LP90 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G376820 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.9e-80
Identity = 159/177 (89.83%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGRSRA  ES I+ GES+P+RM YDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGEEEGTRNELLYEAFKIFDVD 120
           EKAKGVVEKLGLI   EEKEKFELAA E GDEVAVEE+IGEEEG RNELLYEAFKIFDVD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEMIGEEEGKRNELLYEAFKIFDVD 120

Query: 121 GDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 178
           GDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 GDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

[Close]

BLAST of Lsi08G011080 vs. ExPASy TrEMBL
Match: A0A6J1GHP6 (calmodulin-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111454254 PE=4 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 4.2e-74
Identity = 153/178 (85.96%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGRSRA A+S  + G   PHR+ +DDVLLRALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSRA-ADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIG--EEEGTRNELLYEAFKIFD 120
           EKAKGVVEKLGLI+SEEEKE FEL A   GDEVAVEEVIG  EEEG RNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 177
           VDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 177

[Close]

BLAST of Lsi08G011080 vs. ExPASy TrEMBL
Match: A0A6J1KJR1 (calmodulin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111496334 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.7e-73
Identity = 153/178 (85.96%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGRSRA A+SA + G   P R+ +DDVLLRALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSRA-ADSAADGGAPSPCRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIG--EEEGTRNELLYEAFKIFD 120
           EKAKGVVEKLGLI+SEEEKE FEL A   GDEVAVEEVIG  EEEG RNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 177
           VDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 177

[Close]

BLAST of Lsi08G011080 vs. NCBI nr
Match: XP_038886166.1 (calmodulin-like protein 2 [Benincasa hispida])

HSP 1 Score: 317.4 bits (812), Expect = 7.9e-83
Identity = 164/178 (92.13%), Postives = 170/178 (95.51%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT GGRSR+ AES I+DG++RPHRM YDDVLLRALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTFGGRSRSAAESTIDDGKTRPHRMVYDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELA-AAEDGDEVAVEEVIGEEEGTRNELLYEAFKIFDV 120
           EKAKGVVEKLGLI+SEEEK +FELA AAE GDEVAVEEVIGEEEG RNELLYEAFKIFDV
Sbjct: 61  EKAKGVVEKLGLIQSEEEKARFELATAAEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDV 120

Query: 121 DGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 178
           DGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 DGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 178

[Close]

BLAST of Lsi08G011080 vs. NCBI nr
Match: XP_008445056.1 (PREDICTED: calmodulin-like protein 7 [Cucumis melo] >KAA0065018.1 calmodulin-like protein 7 [Cucumis melo var. makuwa])

HSP 1 Score: 310.1 bits (793), Expect = 1.3e-80
Identity = 161/177 (90.96%), Postives = 166/177 (93.79%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGRSRA AES I+ GES+P+RM YDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAAESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGEEEGTRNELLYEAFKIFDVD 120
           EKAKGVVEKLGLI   EEKEKFELAA E GDEVAVEEVIGEEEG RNELLYEAFKIFDVD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEVIGEEEGKRNELLYEAFKIFDVD 120

Query: 121 GDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 178
           GDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 GDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

[Close]

BLAST of Lsi08G011080 vs. NCBI nr
Match: XP_004138884.1 (calmodulin-like protein 2 [Cucumis sativus] >KGN62844.1 hypothetical protein Csa_021949 [Cucumis sativus])

HSP 1 Score: 307.4 bits (786), Expect = 8.1e-80
Identity = 159/177 (89.83%), Postives = 165/177 (93.22%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQTLGGRSRA  ES I+ GES+P+RM YDDVLLRALITVFGMRENGRIK 
Sbjct: 1   MGISRFIGDFLQTLGGRSRAAVESTIDAGESKPNRMVYDDVLLRALITVFGMRENGRIKT 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGEEEGTRNELLYEAFKIFDVD 120
           EKAKGVVEKLGLI   EEKEKFELAA E GDEVAVEE+IGEEEG RNELLYEAFKIFDVD
Sbjct: 61  EKAKGVVEKLGLI---EEKEKFELAAGEGGDEVAVEEMIGEEEGKRNELLYEAFKIFDVD 120

Query: 121 GDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 178
           GDGFIDT+ELKRV+DCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL
Sbjct: 121 GDGFIDTIELKRVIDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKCL 174

[Close]

BLAST of Lsi08G011080 vs. NCBI nr
Match: XP_022951438.1 (calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 287.3 bits (734), Expect = 8.7e-74
Identity = 153/178 (85.96%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+GGRSRA A+S  + G   PHR+ +DDVLLRALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVGGRSRA-ADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIG--EEEGTRNELLYEAFKIFD 120
           EKAKGVVEKLGLI+SEEEKE FEL A   GDEVAVEEVIG  EEEG RNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 177
           VDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 177

[Close]

BLAST of Lsi08G011080 vs. NCBI nr
Match: KAG6585405.1 (putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020321.1 putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 285.0 bits (728), Expect = 4.3e-73
Identity = 152/178 (85.39%), Postives = 160/178 (89.89%), Query Frame = 0


Query: 1   MGISRFIGDFLQTLGGRSRATAESAINDGESRPHRMAYDDVLLRALITVFGMRENGRIKK 60
           MGISRFIGDFLQT+ GRSRA A+S  + G   PHR+ +DDVLLRALITVFGMRENGRIKK
Sbjct: 1   MGISRFIGDFLQTVSGRSRA-ADSTADGGAPSPHRVIHDDVLLRALITVFGMRENGRIKK 60

Query: 61  EKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIG--EEEGTRNELLYEAFKIFD 120
           EKAKGVVEKLGLI+SEEEKE FEL A   GDEVAVEEVIG  EEEG RNELL+EAFKIFD
Sbjct: 61  EKAKGVVEKLGLIKSEEEKEGFELPADGGGDEVAVEEVIGEMEEEGKRNELLWEAFKIFD 120

Query: 121 VDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFELMMGVKC 177
           VDGDGFIDTVELKRVL+CLGLDKGWGIREIEKMVSVVDVNLDGKVDF EFELMMGVKC
Sbjct: 121 VDGDGFIDTVELKRVLECLGLDKGWGIREIEKMVSVVDVNLDGKVDFGEFELMMGVKC 177

[Close]

BLAST of Lsi08G011080 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 73.6 bits (179), Expect = 1.8e-13
Identity = 37/70 (52.86%), Postives = 48/70 (68.57%), Query Frame = 0


Query: 103 EGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGK 162
           +G+    L EAF +FD DGDGFI  VEL++VL  LGL +   I ++EKM+  VD N DG+
Sbjct: 105 DGSPESDLEEAFNVFDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGR 164

Query: 163 VDFSEFELMM 173
           VDF EF+ MM
Sbjct: 165 VDFFEFKNMM 174

[Close]

BLAST of Lsi08G011080 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-12
Identity = 46/136 (33.82%), Postives = 72/136 (52.94%), Query Frame = 0


Query: 43  LRALITVFGMRENGRIKKEKAKGVVEKLGLIESEEEKEKFELAAAEDGDEVAVEEVIGE- 102
           L  +  +F    +G+I K + K   + +G++  E E  +       +GD     +  G  
Sbjct: 6   LSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSL 65

Query: 103 -----EEGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVD 162
                EE    E + EAF++FD +GDGFI   EL+ VL  +GL +G  + + +KM+S VD
Sbjct: 66  YQEMVEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVD 125

Query: 163 VNLDGKVDFSEFELMM 173
           V+ DG V+F EF+ MM
Sbjct: 126 VDGDGMVNFKEFKQMM 141

[Close]

BLAST of Lsi08G011080 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 69.3 bits (168), Expect = 3.5e-12
Identity = 49/143 (34.27%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 39  DDVLLRALITVFGMRENGRIKKEKAKGVVEKLGLIESEEEKEKFELAAAEDGDE-VAVEE 98
           D   L  +  +F    +G+I  ++     + LG+I  E+E  +       +GD  V +EE
Sbjct: 2   DSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEE 61

Query: 99  --------VIGEEEGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIE 158
                   ++ +E+    E + EAF +FD +GDGFI   ELK VL  LGL +G  + E  
Sbjct: 62  FGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECR 121

Query: 159 KMVSVVDVNLDGKVDFSEFELMM 173
           KM+  VDV+ DG+V++ EF  MM
Sbjct: 122 KMIMQVDVDGDGRVNYMEFRQMM 144

[Close]

BLAST of Lsi08G011080 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 68.9 bits (167), Expect = 4.5e-12
Identity = 35/63 (55.56%), Postives = 44/63 (69.84%), Query Frame = 0


Query: 110 LYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMVSVVDVNLDGKVDFSEFE 169
           L EAFK+FD +GDGFI   EL+ VL  LGL +G  +  +EKM+  VD N DG+VDF EF+
Sbjct: 121 LAEAFKVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFK 180

Query: 170 LMM 173
            MM
Sbjct: 181 NMM 183

[Close]

BLAST of Lsi08G011080 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 67.4 bits (163), Expect = 1.3e-11
Identity = 48/140 (34.29%), Postives = 73/140 (52.14%), Query Frame = 0


Query: 39  DDVLLRALITVFGMRENGRIKKEKAKGVVEKLGL-IESEEEKEKFELAAAEDGDEVAVEE 98
           D   L  +  +F    +G+I K++    +E LG+ I  ++  +  E         V +EE
Sbjct: 2   DQAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEE 61

Query: 99  VIG-----EEEGTRNELLYEAFKIFDVDGDGFIDTVELKRVLDCLGLDKGWGIREIEKMV 158
             G      EE    E + EAF +FD + DGFI   EL+ VL  LGL +G  + + ++M+
Sbjct: 62  FGGLYQTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMI 121

Query: 159 SVVDVNLDGKVDFSEFELMM 173
           S VDV+ DG V+F EF+ MM
Sbjct: 122 SKVDVDGDGMVNFKEFKQMM 141

The following BLAST results are available for this feature:

BLAST of Lsi08G011080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FI19	2.6e-12	52.86	Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ...	[more]
Q9SU00	2.2e-11	33.82	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q84UL5	3.7e-11	47.62	Probable calcium-binding protein CML32 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q7XHW4	4.9e-11	47.83	Probable calcium-binding protein CML24 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9ZR02	4.9e-11	34.27	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of Lsi08G011080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VBM3	6.1e-81	90.96	Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A1S3BBS0	6.1e-81	90.96	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103488209 PE=4 SV=1	[more]
A0A0A0LP90	3.9e-80	89.83	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G376820 PE=4 SV=1	[more]
A0A6J1GHP6	4.2e-74	85.96	calmodulin-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111454254 PE=4 SV=...	[more]
A0A6J1KJR1	2.7e-73	85.96	calmodulin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111496334 PE=4 SV=1	[more]

BLAST of Lsi08G011080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886166.1	7.9e-83	92.13	calmodulin-like protein 2 [Benincasa hispida]	[more]
XP_008445056.1	1.3e-80	90.96	PREDICTED: calmodulin-like protein 7 [Cucumis melo] >KAA0065018.1 calmodulin-lik...	[more]
XP_004138884.1	8.1e-80	89.83	calmodulin-like protein 2 [Cucumis sativus] >KGN62844.1 hypothetical protein Csa...	[more]
XP_022951438.1	8.7e-74	85.96	calmodulin-like protein 2 [Cucurbita moschata] >XP_023537761.1 calmodulin-like p...	[more]
KAG6585405.1	4.3e-73	85.39	putative calcium-binding protein CML27, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Lsi08G011080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44460.1	1.8e-13	52.86	calmodulin like 43	[more]
AT4G12860.1	1.6e-12	33.82	EF hand calcium-binding protein family	[more]
AT4G03290.1	3.5e-12	34.27	EF hand calcium-binding protein family	[more]
AT4G20780.1	4.5e-12	55.56	calmodulin like 42	[more]
AT3G07490.1	1.3e-11	34.29	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 147..175 e-value: 0.71 score: 18.9 coord: 109..137 e-value: 0.0018 score: 27.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 109..172 e-value: 2.0E-10 score: 40.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 105..140 score: 14.234451
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 143..177 score: 8.962221
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 112..168 e-value: 8.09349E-14 score: 61.0245
None	No IPR available	GENE3D	1.10.238.10		coord: 17..175 e-value: 6.0E-20 score: 74.0
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 88..173
None	No IPR available	PANTHER	PTHR10891:SF743	CALCIUM-BINDING PROTEIN CML42	coord: 88..173
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 118..130
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 49..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G011080.1	Lsi08G011080.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding