Lsi08G010730 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G010730
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMalic enzyme
Locationchr08: 19289013 .. 19294657 (-)
RNA-Seq ExpressionLsi08G010730
SyntenyLsi08G010730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGTTTCTCACTCAAGAAAACGCGGAAACCTTGCAATTTTTTTTTCTTGTAAACTCAATTTCTATTTTCATACACGTTCTCACAGATATGCTTTCCTTCCTCCGACATCAGTATCTGGTAATAATAATAATAATAAAAAAAAGTTCACTTTTTTAATTTTAATTTTAATTTTTAATTTTGGGGGTTTGTTGAATTCTTTGAATCAATTAGTCCACGTGGGTCGTTTGTGCTCTTCACATTGTCGCCATAATTCGTTGACAAAACAACTCAATCAAGAATAAGCAAAGAATTTTACACTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTCATTATAAAAGCTGAATCTTGCTTCTTCCATTTCCACCAAATTCCTTCTGTATTTTCAAAAGGGTGTATTTCCATTCTCTCTGTGTTGCCTTTTTTCCTTTAGCTTTCTGCCATTTTGCAGAGAAATTTGGGTTGTAGGGGAAGGGGATTTGAGATTGAAGTTTTGGGATTAATGGGGAGTTTAATGAAGAAGACAATGAGCAGTGGTAGTGTTCATATAGAGATAGAAGAAATTGACAATAAGTTTGAAGAAGCTTATGGAGAACATAATGCCACTGAAGATCAGCTCATTACTCCTTGGACTGTCTCTGTTGCTAGGTAAGGGCTTTAAGGAAGATCTTTGCTGCTAAAAAAAGTGTTCTGTTTTTTTGGGGTTGTCTTTGCATTTGTTCTAATCTAACTTTAAACTTCCACATATTTTTGGCTGTTTTCTTTTTTGTTTTAGTGGTTATTCCTTATTGCGTGATCCGATCTACAACAAAGGACTTGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCACTTCTTGATCAAGAACTTCAGGTAAAGTTTAGATCTCTTTTTAGTTCTTCCCTTTTCTTTTTTCTTGATTTTTCTTCTTTATGTTCTTGATTTGGATTCTTTGTTAAAATGGCTTTTTTTTGGGAAGCAGGAAAGGAGGATGATGCATAATCTTCGAAACTATGAAGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGTTTGATATTATCTTCACTCATTTGTTGATAAATTTTAGTTTTCCTATTTGGTTCTTCCTTCTGCACCTAATGAGTTAAGACCATTAGTAGGAGGAAGAAATGAGTATCACTGATATAAGGATGTGGGGTGTTTGGACTTTGGGTATCCTATGTGGGGTTTGTCTTTGGAAGTGAACTTTTAAGGCTAAATTATAAAAAATATCTCTAAACTATCTTCTTTGTTTAAAAATATTCTTGTTTTTTCAAAAGTTGTAAGATTTTCTTGACCTTTCATAAATGTTTCAAAATTACCCTAGAGTACAAATTTTTAAGGTTTCTTATTGGAGTTGGGACGTGGAATGATGTGGCGGTGTCTTTGAATGGGTGGTGATCCAAATTTTCGTTGTAGGTTACTGCCGCATAATTCTTAGTCCCAATTTTGGTTCAAAATTCTAAAGAATTCTACTCGAGGGTAGTTTCAAAATGTTTATTAAAGGTCAAAGAATTCTACTTCAGGAGTAAATATAAGGTTTTAAAAGCAGAGGTGTTGCTGGGTTCTTGTTCTTTTAGTCATATCATTGAACTGCACACAACAAGATTGTAAATCTTAGAATCTATGAGACTTCCTTATCCCAATTGATGTTTCTACTTGTAGCTTCGTTTTACTCGGCCATACCGAGAATTTTCCTTCATGTCTACTGTCATTTGATCTTGCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATCGATAACGTTGAAGAACTGCTTCCAGTTGTGTACACTCCAACTGTAGGCGAAGCTTGCCAGAAGTATGGGAGTATTTACAGGCGACCTCAGGGCCTTTTCATCAGTTTGAAAGAGAAGTATGTTTTTAAACAAACATTTAGAATGTATGATTAGATGCATTAGTTCAGAACTGATAATCAACAATTTCCCTTTTCAGGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATTGTTGTTACCGATGGTGAGCGGATTCTAGGCCTTGGGGATCTCGGATGCCAGGTAACAGAACTTACTGCTTCCATTCAGCTGATGAACCTTCATTTTTACTTCTATCCTTTAGTTTACTGTTTACTATTCTCATTTTGTTAAAATGATATCTTTCAGGGCATGGGGATTCCTGTGGGGAAGCTTTCTTTGTATACAGCACTTGGAGGAATTAGACCCTCAGCTGTAAGCAATGCTTTAGTTTATATACTTCTGTTAATCTCTGCCTCCCTATGTTTATCATCAGTAATCTGAATTCAGCAAAATGTTGATACCTGGATTAGTCAGCTTCGCTTTCAAGCAAGTACTAAGCCAAGCAAGGATTAAAGTTTATAGTTGTGTATTTTGTGCAGTGCTTGCCAATAACCATTGACGTCGGTACGAACAACGAGCAGCTTCTTAACGATGAGTTCTACATTGGACTTAAACATAAACGGGCCAGAGGACAGGTGAAACATTCTCTTGCTCCCTTTTTACCTCCGGACTTTATGATTTCAATCCATTCATGCATGAACGTTTTAATCAATTTTTGTTTCTTTTTCCTTATTCAGGAATACATGGAACTTTTAGATGAGTTCATGTATGCAGTGAAGAAGAACTATGGAGAGAAAGTCCTTATACAGGTTATTTGGCAATTTCAACTTACAGTAGGATAGAACTTGCTTGGTGTTATGTACAAGTGATTCAATGTATCACTAAGCATTTCTTACTTATTAAACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTTAACGACGACATTCAAGTACAAAACATCCGCTTCGCTTTCAATTGACTTCTAACTTCCATATGTTTCAAGAGTAGCTCTCTAACACTGTCTCTCATTCCGACATTTAGGGTACGGCATCTGTTGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTGTTGGGGGGACATTAGCTGACCACACTTTCTTGTTCCTTGGAGCTGGAGAGGTGAGTTAAAAACGAGTTATATTCGAAGTGTTAAATTGTTTTCATCGACGTTGGCACGTATTTCCTTGATGATCATCTGCTTAAGTTTCATGTCTTGTCCCCAAAATTTTGATTCACAACATTATATGTTTTCTTTGGCACGTTTCTTATTTCTGTCATTTGACTGTTCTAGGCTGGAACTGGTATAGCTGAGCTTATTGCCTTGGAGATTTCAAAACAGGTATGAGCTTACTTCTACTTCTCCTCTTTTATTTGTTCTGAATCAATCAAATGCATTTATGGCTTATGATTTTACTTGTTTTTGGCAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTTGTGGACTCAAAGGTTGTTTGGAATGACACACTACAATATTGTGCCTTTACACTCAATGTTATTTCCTGTTCTAAAAGAATTATATCCATTTGTTACAGGGGCTGATCGTTCGATCCCGCTTGGAATCCCTTCAACATTTTAAGAAGCCATGGGCTCATGACCATGAATCCATCAAGGATCTTTACGGTGCCGTCCAGGTTTGTGATGGATATCGATTATGCTTTCATTATTTACGTTCTTCAAAGTATATGAGACCATTTTCTAACCAAATCTTAAAACTTTAAATTATATAGGCAATCAAGCCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAAACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGGTTCTTCCTTGTTCCCTCTAACTTTGTTATTTCTCAATTCTAAGACAAGAAATTATTTAAGATTAACTCAAGTATCGATTGCTATGCAGAAACCACTCATCCTTGCTCTCTCGAATCCTACGTCGCAATCGGAGTGTACGGCTGAAGAAGCCTATACATGGAGCCAGGTACAATCCAAATCTTTCAAAACAGCAGGCATCATTTTCTGTTTTTTGTAGATCACATCATAACAAAACTTCACCAAAAACGTGCAGGGCCGAGCGATCTTTGCTAGTGGAAGTCCATTTGACCCTTTTGAATATGATGGGAAAGTTTTTGTGCCTGGCCAGGTACCTTTCATACTTCAAAAACCATTTTTCTTAGTAACCATTCAATTTTAACAACATAGTTTGATCTTATGAGCTTGCTTTTCTCTTGCTAATGTCATTTAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGAACGATTCGTGTGCACGATGACATGCTCTTAGCAGCCTGTAAGTGAAATTTTGGTTAATTGATCCAACTAATGATGCTTGTTTTGTGCCAATCTTTATGAAGTATATGCATATCTATAGAATATTAGTTGTCGGGTTGGAGCTCGATTTAACGGTTTTTTCCCCTCTTTGCAGCGGAGGCTTTGGCTGCCCAAGTGAGTCAGGAAAACTATGACAAGGGATTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCTAAAGTTGCCGCTAAGGCTTATGAACTTGGTTAGCTTCTCTTCCATTTTCTTCACAATGTGATTTAAATCATTCATAGATAAACCAATGACAGGAAGAGTTCACTAACACATGCTATTACATTGCAGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTTGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTACCGCAGCTACCTTTAAGCGAATATTGTAGCTTCACCCTTAAAATTTTTTTCCTCATTTTCTTTTTTTGTTTGTGTAATTTGTTGTTTTTAGTCCTGGCATTAGGCACAGGACATGTCTTAAAAGAGCTTGTTACTTTTCTTTATCTTATTGTTCTGTTTCTGAAGTTGTTTATCTCTTATGTAACATTCATTTGTTATTTAGATTGAGTAATGTAATAGGCACTTCCAATTCAATGAGATTACTTGTGAAATATATAATAATGAATTGAGCAGATATTGACTGCTTGATCTGATGTAACCATTTGAAAATCATATTCCTTCCCTTATTAGTCTCA

mRNA sequence

CTGTTTCTCACTCAAGAAAACGCGGAAACCTTGCAATTTTTTTTTCTTGTAAACTCAATTTCTATTTTCATACACGTTCTCACAGATATGCTTTCCTTCCTCCGACATCAGTATCTGAGAAATTTGGGTTGTAGGGGAAGGGGATTTGAGATTGAAGTTTTGGGATTAATGGGGAGTTTAATGAAGAAGACAATGAGCAGTGGTAGTGTTCATATAGAGATAGAAGAAATTGACAATAAGTTTGAAGAAGCTTATGGAGAACATAATGCCACTGAAGATCAGCTCATTACTCCTTGGACTGTCTCTGTTGCTAGTGGTTATTCCTTATTGCGTGATCCGATCTACAACAAAGGACTTGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCACTTCTTGATCAAGAACTTCAGGAAAGGAGGATGATGCATAATCTTCGAAACTATGAAGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATCGATAACGTTGAAGAACTGCTTCCAGTTGTGTACACTCCAACTGTAGGCGAAGCTTGCCAGAAGTATGGGAGTATTTACAGGCGACCTCAGGGCCTTTTCATCAGTTTGAAAGAGAAGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATTGTTGTTACCGATGGTGAGCGGATTCTAGGCCTTGGGGATCTCGGATGCCAGGGCATGGGGATTCCTGTGGGGAAGCTTTCTTTGTATACAGCACTTGGAGGAATTAGACCCTCAGCTTGCTTGCCAATAACCATTGACGTCGGTACGAACAACGAGCAGCTTCTTAACGATGAGTTCTACATTGGACTTAAACATAAACGGGCCAGAGGACAGGTGAAACATTCTCTTGCTCCCTTTTTACCTCCGGACTTTATGATTTCAATCCATTCATGCATGAACGTTTTAATCAATTTTTGTTTCTTTTTCCTTATTCAGGAATACATGGAACTTTTAGATGAGTTCATGTATGCAGTGAAGAAGAACTATGGAGAGAAAGTCCTTATACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTTAACGACGACATTCAAGGTACGGCATCTGTTGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTGTTGGGGGGACATTAGCTGACCACACTTTCTTGTTCCTTGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCCTTGGAGATTTCAAAACAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTTGTGGACTCAAAGGGGCTGATCGTTCGATCCCGCTTGGAATCCCTTCAACATTTTAAGAAGCCATGGGCTCATGACCATGAATCCATCAAGGATCTTTACGGTGCCGTCCAGGCAATCAAGCCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAAACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGAAACCACTCATCCTTGCTCTCTCGAATCCTACGTCGCAATCGGAGTGTACGGCTGAAGAAGCCTATACATGGAGCCAGGGCCGAGCGATCTTTGCTAGTGGAAGTCCATTTGACCCTTTTGAATATGATGGGAAAGTTTTTGTGCCTGGCCAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGAACGATTCGTGTGCACGATGACATGCTCTTAGCAGCCTCGGAGGCTTTGGCTGCCCAAGTGAGTCAGGAAAACTATGACAAGGGATTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCTAAAGTTGCCGCTAAGGCTTATGAACTTGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTTGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTACCGCAGCTACCTTTAAGCGAATATTGTAGCTTCACCCTTAAAATTTTTTTCCTCATTTTCTTTTTTTGTTTGTGTAATTTGTTGTTTTTAGTCCTGGCATTAGGCACAGGACATGTCTTAAAAGAGCTTGTTACTTTTCTTTATCTTATTGTTCTGTTTCTGAAGTTGTTTATCTCTTATGTAACATTCATTTGTTATTTAGATTGAGTAATGTAATAGGCACTTCCAATTCAATGAGATTACTTGTGAAATATATAATAATGAATTGAGCAGATATTGACTGCTTGATCTGATGTAACCATTTGAAAATCATATTCCTTCCCTTATTAGTCTCA

Coding sequence (CDS)

ATGCTTTCCTTCCTCCGACATCAGTATCTGAGAAATTTGGGTTGTAGGGGAAGGGGATTTGAGATTGAAGTTTTGGGATTAATGGGGAGTTTAATGAAGAAGACAATGAGCAGTGGTAGTGTTCATATAGAGATAGAAGAAATTGACAATAAGTTTGAAGAAGCTTATGGAGAACATAATGCCACTGAAGATCAGCTCATTACTCCTTGGACTGTCTCTGTTGCTAGTGGTTATTCCTTATTGCGTGATCCGATCTACAACAAAGGACTTGCCTTTAGTGAAAAAGAAAGAGATGCTCATTACTTGCGTGGCTTGTTGCCACCAGCACTTCTTGATCAAGAACTTCAGGAAAGGAGGATGATGCATAATCTTCGAAACTATGAAGTTCCATTACACAGATATATTGCCATGATGGATCTTCAGGAGAGAAATGAAAGGTTGTTCTACAAGCTTCTCATCGATAACGTTGAAGAACTGCTTCCAGTTGTGTACACTCCAACTGTAGGCGAAGCTTGCCAGAAGTATGGGAGTATTTACAGGCGACCTCAGGGCCTTTTCATCAGTTTGAAAGAGAAGGGCAAGATTCTTGAAGTTTTGAAGAACTGGCCAGAAAGGAACATTCAAGTTATTGTTGTTACCGATGGTGAGCGGATTCTAGGCCTTGGGGATCTCGGATGCCAGGGCATGGGGATTCCTGTGGGGAAGCTTTCTTTGTATACAGCACTTGGAGGAATTAGACCCTCAGCTTGCTTGCCAATAACCATTGACGTCGGTACGAACAACGAGCAGCTTCTTAACGATGAGTTCTACATTGGACTTAAACATAAACGGGCCAGAGGACAGGTGAAACATTCTCTTGCTCCCTTTTTACCTCCGGACTTTATGATTTCAATCCATTCATGCATGAACGTTTTAATCAATTTTTGTTTCTTTTTCCTTATTCAGGAATACATGGAACTTTTAGATGAGTTCATGTATGCAGTGAAGAAGAACTATGGAGAGAAAGTCCTTATACAGTTTGAAGATTTTGCAAACCACAATGCATTCGAGTTGCTTTCAAGATATAGCTCGTCACACCTTGTGTTTAACGACGACATTCAAGGTACGGCATCTGTTGTCGTAGCAGGACTACTTGCAGCTCTTAAACTTGTTGGGGGGACATTAGCTGACCACACTTTCTTGTTCCTTGGAGCTGGAGAGGCTGGAACTGGTATAGCTGAGCTTATTGCCTTGGAGATTTCAAAACAGACTGGAGCTCCAACTGAAGAGACTCGCAAGAAGATTTGGCTTGTGGACTCAAAGGGGCTGATCGTTCGATCCCGCTTGGAATCCCTTCAACATTTTAAGAAGCCATGGGCTCATGACCATGAATCCATCAAGGATCTTTACGGTGCCGTCCAGGCAATCAAGCCAACAGTGTTGATAGGAACATCTGGTGTTGGGAAAACATTTACAAAGGAAGTTGTGGAGGCCATGGCATCCTTCAATGAGAAACCACTCATCCTTGCTCTCTCGAATCCTACGTCGCAATCGGAGTGTACGGCTGAAGAAGCCTATACATGGAGCCAGGGCCGAGCGATCTTTGCTAGTGGAAGTCCATTTGACCCTTTTGAATATGATGGGAAAGTTTTTGTGCCTGGCCAGGCAAACAATGCTTACATATTCCCTGGATTTGGCTTGGGTCTGATCATGTCGGGAACGATTCGTGTGCACGATGACATGCTCTTAGCAGCCTCGGAGGCTTTGGCTGCCCAAGTGAGTCAGGAAAACTATGACAAGGGATTGATTTACCCTCCTTTTACCAACATCAGAAAGATATCTGCTAACATTGCTGCTAAAGTTGCCGCTAAGGCTTATGAACTTGGTCTGGCTTCTCGTCTTCCTCGACCAAAGGATCTTGTAAAATTTGCCGAGAGCTGCATGTACAGCCCCCGCTACCGCAGCTACCTTTAA

Protein sequence

MLSFLRHQYLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Homology
BLAST of Lsi08G010730 vs. ExPASy Swiss-Prot
Match: P12628 (NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 7.5e-283
Identity = 489/596 (82.05%), Postives = 528/596 (88.59%), Query Frame = 0

Query: 54  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQ 113
           + YGE +ATED LITPWT SVASG SLLRDP YNKGLAF+E ERDAHYLRGLLPP++ +Q
Sbjct: 27  DLYGEDSATEDHLITPWTFSVASGCSLLRDPRYNKGLAFTEGERDAHYLRGLLPPSVFNQ 86

Query: 114 ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 173
           ELQE+R+MHNLR YEVPLHRY+A+MDLQERNERLFYKLLIDNV ELLPVVYTPTVGEACQ
Sbjct: 87  ELQEKRLMHNLRQYEVPLHRYMALMDLQERNERLFYKLLIDNVAELLPVVYTPTVGEACQ 146

Query: 174 KYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 233
           KYGSI+RRPQGL+ISLKEKGKILEVLKNWPE++IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 147 KYGSIFRRPQGLYISLKEKGKILEVLKNWPEKSIQVIVVTDGERILGLGDLGCQGMGIPV 206

Query: 234 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPD 293
           GKLSLYTALGG+RPS+CLP+TIDVGTNNE+LLNDEFYIGL+ +RA G             
Sbjct: 207 GKLSLYTALGGVRPSSCLPVTIDVGTNNEKLLNDEFYIGLRQRRATG------------- 266

Query: 294 FMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLS 353
                                QEY   LDEFM AVK+NYGEKVL+QFEDFANHNAF+LL 
Sbjct: 267 ---------------------QEYATFLDEFMRAVKQNYGEKVLVQFEDFANHNAFDLLE 326

Query: 354 RYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEI 413
           +YSSSHLVFNDDIQGTASVV+AGLLA+LKLVGGTLADHTFLFLGAGEAGTGIAELIA+E+
Sbjct: 327 KYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIAVEV 386

Query: 414 SKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTV 473
           SKQT AP EETRKKIWLVDSKGLIV SRLESLQ FKKPWAH+HE +K L  AV+AIKPTV
Sbjct: 387 SKQTKAPVEETRKKIWLVDSKGLIVSSRLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTV 446

Query: 474 LIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSP 533
           LIG+SG GKTFTKEVVE MAS NEKPLILALSNPTSQSECTAEEAYTWS+GRAIFASGSP
Sbjct: 447 LIGSSGAGKTFTKEVVETMASLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIFASGSP 506

Query: 534 FDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDK 593
           FDP EY+GK+FVPGQANNAYIFPGFGLGLIMSG IRV D+MLLAASEALAAQVS+ENYDK
Sbjct: 507 FDPVEYEGKLFVPGQANNAYIFPGFGLGLIMSGAIRVRDEMLLAASEALAAQVSEENYDK 566

Query: 594 GLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 650
           GLIYPPFTNIRKISANIAAKVAAKAY+LGLAS L RPKDLVK+AESCMYSP YRSY
Sbjct: 567 GLIYPPFTNIRKISANIAAKVAAKAYDLGLASHLKRPKDLVKYAESCMYSPGYRSY 588

BLAST of Lsi08G010730 vs. ExPASy Swiss-Prot
Match: P36444 (NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA PE=2 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 1.2e-272
Identity = 462/597 (77.39%), Postives = 526/597 (88.11%), Query Frame = 0

Query: 53  EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLD 112
           E+ YGE +ATED  ITPW+VSVASGYSLLRDP +NKGLAF+EKERDAHYLRGLLPP +++
Sbjct: 84  EDVYGEDSATEDHFITPWSVSVASGYSLLRDPHHNKGLAFTEKERDAHYLRGLLPPVVVN 143

Query: 113 QELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEAC 172
            +LQ ++MMHN+R YEVPL RY AMMDLQERNERLFYKLLI+N+EELLP+VYTPTVGEAC
Sbjct: 144 HDLQVKKMMHNIRQYEVPLQRYQAMMDLQERNERLFYKLLIENIEELLPIVYTPTVGEAC 203

Query: 173 QKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 232
           QKYG+I++ PQGL+ISLK+KGK+LE+LKNWP++ IQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 204 QKYGTIFKNPQGLYISLKDKGKVLEILKNWPQKKIQVIVVTDGERILGLGDLGCQGMGIP 263

Query: 233 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPP 292
           VGKLSLYTALGGIRPSACLPITIDVGTNNE++LNDEFYIGL+ +RA G            
Sbjct: 264 VGKLSLYTALGGIRPSACLPITIDVGTNNEKMLNDEFYIGLRQRRASG------------ 323

Query: 293 DFMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELL 352
                                 +EY EL++EFM AVK+NYGEKVLIQFEDFANHNAF+LL
Sbjct: 324 ----------------------KEYAELMNEFMSAVKQNYGEKVLIQFEDFANHNAFDLL 383

Query: 353 SRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALE 412
            +Y ++HLVFNDDIQGTASVV+AGL++ALKLVGG+LADH FLFLGAGEAGTGIAELIALE
Sbjct: 384 EKYRTTHLVFNDDIQGTASVVLAGLISALKLVGGSLADHKFLFLGAGEAGTGIAELIALE 443

Query: 413 ISKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPT 472
           ISKQT AP EETRKKIWLVDSKGLIVRSRL+SLQHFKKPWAHDHE +     AV+AIKPT
Sbjct: 444 ISKQTNAPLEETRKKIWLVDSKGLIVRSRLDSLQHFKKPWAHDHEPVNKFLDAVKAIKPT 503

Query: 473 VLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGS 532
           VLIG+SG G+TFTKEVVEAM+SFNEKP+ILALSNPTSQSECTAE+AYTWS+GR IFASGS
Sbjct: 504 VLIGSSGAGQTFTKEVVEAMSSFNEKPIILALSNPTSQSECTAEQAYTWSEGRTIFASGS 563

Query: 533 PFDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYD 592
           PF P EY+GKV+V GQ+NNAYIFPGFGLGLI+SG IRVHD+MLLAASEALA QV+QE++D
Sbjct: 564 PFAPVEYNGKVYVSGQSNNAYIFPGFGLGLIISGAIRVHDEMLLAASEALAEQVTQEHFD 623

Query: 593 KGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 650
            GLIYPPFTNIRKISA+IAAKVAAKAYELGLASRLP+P++LV +AESCMYSP+YR+Y
Sbjct: 624 NGLIYPPFTNIRKISAHIAAKVAAKAYELGLASRLPQPENLVAYAESCMYSPKYRNY 646

BLAST of Lsi08G010730 vs. ExPASy Swiss-Prot
Match: Q9XGZ0 (NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME3 PE=1 SV=1)

HSP 1 Score: 937.6 bits (2422), Expect = 7.7e-272
Identity = 467/596 (78.36%), Postives = 514/596 (86.24%), Query Frame = 0

Query: 54  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQ 113
           + YGE +AT DQL+TPW  SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP +L Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85

Query: 114 ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 173
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 174 KYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 233
           KYGSIYRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 234 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPD 293
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGLK KRA G             
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANG------------- 265

Query: 294 FMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLS 353
                                +EY E L EFM AVK+NYGEKVL+QFEDFANH+AFELLS
Sbjct: 266 ---------------------EEYAEFLQEFMCAVKQNYGEKVLVQFEDFANHHAFELLS 325

Query: 354 RYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEI 413
           +Y SSHLVFNDDIQGTASVV+AGL+AA K++G +LADHTFLFLGAGEAGTGIAELIAL+I
Sbjct: 326 KYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLADHTFLFLGAGEAGTGIAELIALKI 385

Query: 414 SKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTV 473
           SK+TG P +ETRKKIWLVDSKGLIV  R ESLQHFK+PWAHDH+ +K+L  AV AIKPTV
Sbjct: 386 SKETGKPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWAHDHKPVKELLAAVNAIKPTV 445

Query: 474 LIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSP 533
           LIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNPTSQ+ECTAEEAYTW++GRAIFASGSP
Sbjct: 446 LIGTSGVGKTFTKEVVEAMATLNEKPLILALSNPTSQAECTAEEAYTWTKGRAIFASGSP 505

Query: 534 FDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDK 593
           FDP +YDGK F PGQANN YIFPG GLGLIMSG IRV DDMLLAASEALA+QV++EN+  
Sbjct: 506 FDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAASEALASQVTEENFAN 565

Query: 594 GLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 650
           GLIYPPF NIRKISANIAA V AK YELGLAS LPRPKDLVK AESCMYSP YR++
Sbjct: 566 GLIYPPFANIRKISANIAASVGAKTYELGLASNLPRPKDLVKMAESCMYSPVYRNF 587

BLAST of Lsi08G010730 vs. ExPASy Swiss-Prot
Match: P51615 (NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 1.3e-271
Identity = 467/597 (78.22%), Postives = 522/597 (87.44%), Query Frame = 0

Query: 53  EEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLD 112
           E+ YGE  ATEDQL+TPWTVSVASGYSLLRDP +NKGLAF++KERDAHYL GLLPP +  
Sbjct: 28  EDLYGEDFATEDQLVTPWTVSVASGYSLLRDPRHNKGLAFNDKERDAHYLCGLLPPVVST 87

Query: 113 QELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEAC 172
           QELQER++M+++R Y+VPL +Y+AMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEAC
Sbjct: 88  QELQERKLMNSIRQYQVPLQKYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEAC 147

Query: 173 QKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIP 232
           QKYGSI+RRPQGL+ISLKEKGKILEVLKNWPER IQVIVVTDGERILGLGDLGCQGMGIP
Sbjct: 148 QKYGSIFRRPQGLYISLKEKGKILEVLKNWPERRIQVIVVTDGERILGLGDLGCQGMGIP 207

Query: 233 VGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPP 292
           VGKLSLYTALGG+RPSACLPITIDVGTNNE+LL +EFYIGLK +RA G            
Sbjct: 208 VGKLSLYTALGGVRPSACLPITIDVGTNNEKLLANEFYIGLKQRRATG------------ 267

Query: 293 DFMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELL 352
                                 +EY E L EFM  VK+NYGEKVLIQFEDFANHNAF+LL
Sbjct: 268 ----------------------KEYSEFLQEFMSPVKQNYGEKVLIQFEDFANHNAFDLL 327

Query: 353 SRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALE 412
           ++Y ++HL FNDDIQGTASVV+AG+++AL+L+GGTLADH FLFLGAGEAGTGIAELIALE
Sbjct: 328 AKYGTTHLAFNDDIQGTASVVLAGIVSALRLLGGTLADHKFLFLGAGEAGTGIAELIALE 387

Query: 413 ISKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPT 472
           +SKQT  P EETRKKIWLVDSKGLIV SR +SLQ FKKPWAH+HE +KDL  AV+ IKPT
Sbjct: 388 MSKQTKCPIEETRKKIWLVDSKGLIVGSRKDSLQQFKKPWAHEHEPVKDLLDAVKVIKPT 447

Query: 473 VLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGS 532
           VLIG+SGVGK FTKEV+EAMAS NEKPLILALSNPTSQSECTAEEAYTW+QGRAIFASGS
Sbjct: 448 VLIGSSGVGKAFTKEVIEAMASCNEKPLILALSNPTSQSECTAEEAYTWTQGRAIFASGS 507

Query: 533 PFDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYD 592
           PFDP EY+GK FVPGQANNAYIFPG G+GL++SG IRVHD+MLLAASEALA QV+QEN+D
Sbjct: 508 PFDPVEYNGKTFVPGQANNAYIFPGLGMGLVISGAIRVHDEMLLAASEALARQVTQENFD 567

Query: 593 KGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 650
           KGLIYPPF+NIRKISA+IAA VAAKAYELGLA+RLP+P++LVK+AESCMYSP YRSY
Sbjct: 568 KGLIYPPFSNIRKISAHIAANVAAKAYELGLATRLPQPENLVKYAESCMYSPVYRSY 590

BLAST of Lsi08G010730 vs. ExPASy Swiss-Prot
Match: Q9LYG3 (NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 2.7e-269
Identity = 465/596 (78.02%), Postives = 514/596 (86.24%), Query Frame = 0

Query: 54  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQ 113
           + YGE +AT DQL+TPW  SVASGY+L+RDP YNKGLAF++KERDAHYL GLLPP +L Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQ 85

Query: 114 ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 173
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 174 KYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 233
           KYGSI+R+PQGL+ISL EKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 234 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPD 293
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLNDEFYIGLK +RA G             
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATG------------- 265

Query: 294 FMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLS 353
                                QEY E L EFM AVK+NYGEKVL+QFEDFANHNAF+LLS
Sbjct: 266 ---------------------QEYAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLS 325

Query: 354 RYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEI 413
           +YS SHLVFNDDIQGTASVV+AGL+AA K++G  LADHTFLFLGAGEAGTGIAELIAL+I
Sbjct: 326 KYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLADHTFLFLGAGEAGTGIAELIALKI 385

Query: 414 SKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTV 473
           SK+TGAP  ETRKKIWLVDSKGLIV SR ESLQHFK+PWAH+H+ +KDL GAV AIKPTV
Sbjct: 386 SKETGAPITETRKKIWLVDSKGLIVSSRKESLQHFKQPWAHEHKPVKDLIGAVNAIKPTV 445

Query: 474 LIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSP 533
           LIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNPTSQ+ECTAE+AYTW++GRAIF SGSP
Sbjct: 446 LIGTSGVGQTFTKEVVEAMATNNEKPLILALSNPTSQAECTAEQAYTWTKGRAIFGSGSP 505

Query: 534 FDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDK 593
           FDP  YDGK ++PGQANN YIFPG GLGLIMSG IRV DDMLLAASEALAAQV++E+Y  
Sbjct: 506 FDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAASEALAAQVTEEHYAN 565

Query: 594 GLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 650
           GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS LPR KDLVKFAES MYSP YR+Y
Sbjct: 566 GLIYPPFSNIREISANIAACVAAKTYDLGLASNLPRAKDLVKFAESSMYSPVYRNY 587

BLAST of Lsi08G010730 vs. ExPASy TrEMBL
Match: A0A1S3BB74 (Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103488170 PE=3 SV=1)

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 594/648 (91.67%), Postives = 601/648 (92.75%), Query Frame = 0

Query: 3   SFLRHQYLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHNAT 62
           SFLRHQYLRNL   GR FEIE LG M S MKKTMS+GSVH+E+EEIDNKFEEAYGEHNAT
Sbjct: 4   SFLRHQYLRNLVFIGRRFEIEALGSMESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNAT 63

Query: 63  EDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRMMH 122
           EDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKER+AHYLRGLLPPALLDQELQERRMMH
Sbjct: 64  EDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERNAHYLRGLLPPALLDQELQERRMMH 123

Query: 123 NLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRP 182
           NLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRP
Sbjct: 124 NLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRP 183

Query: 183 QGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 242
           QGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 184 QGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 243

Query: 243 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHSCM 302
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGL+ KRARG                      
Sbjct: 244 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARG---------------------- 303

Query: 303 NVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 362
                       QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF
Sbjct: 304 ------------QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 363

Query: 363 NDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 422
           NDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP E
Sbjct: 364 NDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPIE 423

Query: 423 ETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 482
           ETRKKIWLVDSKGLIV+SR ESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK
Sbjct: 424 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 483

Query: 483 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYDGK 542
           TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDP EYDGK
Sbjct: 484 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 543

Query: 543 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 602
            FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN
Sbjct: 544 AFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 603

Query: 603 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 651
           IRKISANIAA+VAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 604 IRKISANIAAEVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 617

BLAST of Lsi08G010730 vs. ExPASy TrEMBL
Match: A0A6J1BSP9 (Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111004998 PE=3 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 579/650 (89.08%), Postives = 590/650 (90.77%), Query Frame = 0

Query: 1   MLSFLRHQYLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHN 60
           ML FLRHQYLR L   GR        LM   MKKTMS+GSVH+E EEIDNKFEEAYGEHN
Sbjct: 1   MLCFLRHQYLRQLAGSGR-------ALMEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHN 60

Query: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRM 120
           ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPA+LDQELQERRM
Sbjct: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAILDQELQERRM 120

Query: 121 MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 180
           MHNLR Y+VPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR
Sbjct: 121 MHNLRQYDVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 180

Query: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240
           RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT
Sbjct: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240

Query: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHS 300
           ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGL+HKRARG                    
Sbjct: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARG-------------------- 300

Query: 301 CMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360
                         QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL
Sbjct: 301 --------------QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360

Query: 361 VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 420
           VFNDDIQGTASVVVAGLLAALKL+GGTL DHTFLFLGAGEAGTGIAELIALEISKQT AP
Sbjct: 361 VFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGAGEAGTGIAELIALEISKQTKAP 420

Query: 421 TEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 480
            EETR+KIWLVDSKGLIVRSR ESLQHFKKPWAHDHESIKDL GAVQAIKPTVLIGTSGV
Sbjct: 421 VEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTVLIGTSGV 480

Query: 481 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYD 540
           GKTFTKEVVEAM S NEKPLILALSNPTSQSECTAEEAYTWS+GRAI+ASGSPFDP EYD
Sbjct: 481 GKTFTKEVVEAMESLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIYASGSPFDPVEYD 540

Query: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 600
           GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAA+VSQENYDKGLIYPPF
Sbjct: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAEVSQENYDKGLIYPPF 600

Query: 601 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 651
           TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYR+YL
Sbjct: 601 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRNYL 609

BLAST of Lsi08G010730 vs. ExPASy TrEMBL
Match: A0A0A0LLH9 (Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_2G373430 PE=3 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 573/623 (91.97%), Postives = 584/623 (93.74%), Query Frame = 0

Query: 28  MGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87
           M S MKKTMS+GSVH+++EEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 88  KGLAFSEKERDAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 147
           KGLAFSEKERDAHYLRGLLPPA++DQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 148 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 207
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180

Query: 208 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 267
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240

Query: 268 EFYIGLKHKRARGQVKHSLAPFLPPDFMISIHSCMNVLINFCFFFLIQEYMELLDEFMYA 327
           EFYIGL+ KRARG                                  QEYMELLDEFMYA
Sbjct: 241 EFYIGLRQKRARG----------------------------------QEYMELLDEFMYA 300

Query: 328 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGT 387
           VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKL+GGT
Sbjct: 301 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 360

Query: 388 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQH 447
           LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIV+SR ESLQH
Sbjct: 361 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 420

Query: 448 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 507
           FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP
Sbjct: 421 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 480

Query: 508 TSQSECTAEEAYTWSQGRAIFASGSPFDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 567
           TSQSECTAEEAYTWSQGRAIFASGSPFDP EYDGKVFVPGQANNAYIFPGFGLGLIMSGT
Sbjct: 481 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 540

Query: 568 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 627
           IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL
Sbjct: 541 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 589

Query: 628 PRPKDLVKFAESCMYSPRYRSYL 651
           PRPKDLVKFAESCMYSPRYRSYL
Sbjct: 601 PRPKDLVKFAESCMYSPRYRSYL 589

BLAST of Lsi08G010730 vs. ExPASy TrEMBL
Match: A0A5A7VH34 (Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003800 PE=3 SV=1)

HSP 1 Score: 1128.6 bits (2918), Expect = 0.0e+00
Identity = 578/624 (92.63%), Postives = 583/624 (93.43%), Query Frame = 0

Query: 28  MGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87
           M S MKKTMS+GSVH+E+EEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 88  KGLAFSEKERDAHYLRGLLPPALLDQEL-QERRMMHNLRNYEVPLHRYIAMMDLQERNER 147
           KGLAFSEKERDAHYLRGLLPPALLDQEL QERRMMHNLRNYEVPLHRYIAMMDLQERNER
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPALLDQELQQERRMMHNLRNYEVPLHRYIAMMDLQERNER 120

Query: 148 LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERN 207
           LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERN
Sbjct: 121 LFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERN 180

Query: 208 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN 267
           IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN
Sbjct: 181 IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLN 240

Query: 268 DEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHSCMNVLINFCFFFLIQEYMELLDEFMY 327
           DEFYIGL+ KRARG                                  QEYMELLDEFMY
Sbjct: 241 DEFYIGLRQKRARG----------------------------------QEYMELLDEFMY 300

Query: 328 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGG 387
           AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGG
Sbjct: 301 AVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGG 360

Query: 388 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQ 447
           TLADHTFLFLGAGEAGTGIAELIALEISKQTGAP EETRKKIWLVDSKGLIV+SR ESLQ
Sbjct: 361 TLADHTFLFLGAGEAGTGIAELIALEISKQTGAPIEETRKKIWLVDSKGLIVQSRFESLQ 420

Query: 448 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 507
           HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN
Sbjct: 421 HFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSN 480

Query: 508 PTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSG 567
           PTSQSECTAEEAYTWSQGRAIFASGSPFDP EYDGKVFVPGQANNAYIFPGFGLGLIMSG
Sbjct: 481 PTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSG 540

Query: 568 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 627
           TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR
Sbjct: 541 TIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASR 590

Query: 628 LPRPKDLVKFAESCMYSPRYRSYL 651
           LPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 601 LPRPKDLVKFAESCMYSPRYRSYL 590

BLAST of Lsi08G010730 vs. ExPASy TrEMBL
Match: A0A6J1KT37 (Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111496169 PE=3 SV=1)

HSP 1 Score: 1125.9 bits (2911), Expect = 0.0e+00
Identity = 580/651 (89.09%), Postives = 592/651 (90.94%), Query Frame = 0

Query: 1   MLSFLRHQ-YLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEH 60
           MLSFLRH+ YLR            V  LM   MKKTMS+GSVH+EIEEIDNK+EEAYGEH
Sbjct: 1   MLSFLRHRHYLR-----------PVERLMEGAMKKTMSNGSVHMEIEEIDNKYEEAYGEH 60

Query: 61  NATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERR 120
           NATEDQLITPWTVSVASGYSLLRDPIYNKGLAF+EKERDAHYLRGLLPPALL+QELQERR
Sbjct: 61  NATEDQLITPWTVSVASGYSLLRDPIYNKGLAFTEKERDAHYLRGLLPPALLNQELQERR 120

Query: 121 MMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIY 180
           MMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIY
Sbjct: 121 MMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIY 180

Query: 181 RRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 240
           RRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY
Sbjct: 181 RRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLY 240

Query: 241 TALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIH 300
           TALGGIRPSACLPITIDVGTNNEQLLNDEFYIGL+HKRARG                   
Sbjct: 241 TALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARG------------------- 300

Query: 301 SCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSH 360
                          QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSH
Sbjct: 301 ---------------QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSH 360

Query: 361 LVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGA 420
           LVFNDDIQGTASVVVAGLL+ALKLVGGTLADHTFLFLGAGEAGTGIAELIALE+SKQT A
Sbjct: 361 LVFNDDIQGTASVVVAGLLSALKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTQA 420

Query: 421 PTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSG 480
           P EETRKKIWLVDSKGLIVRSR ESLQHFKKPWAHDHESIKDL GAVQAIKPTVLIGTSG
Sbjct: 421 PVEETRKKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTVLIGTSG 480

Query: 481 VGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEY 540
           VGKTFTKEVVEAMAS NEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDP EY
Sbjct: 481 VGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEY 540

Query: 541 DGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPP 600
           DGKV+VPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVS+ENY+KGLIYPP
Sbjct: 541 DGKVYVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSEENYEKGLIYPP 600

Query: 601 FTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 651
           FTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSP YRSYL
Sbjct: 601 FTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPGYRSYL 606

BLAST of Lsi08G010730 vs. NCBI nr
Match: XP_038884200.1 (NADP-dependent malic enzyme [Benincasa hispida])

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 601/650 (92.46%), Postives = 607/650 (93.38%), Query Frame = 0

Query: 1   MLSFLRHQYLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHN 60
           MLSFLRHQYLRNL CRGRGFEIEVLGLM S MKKTMS+GSVH+E+EEIDNKFEEAYGEHN
Sbjct: 1   MLSFLRHQYLRNLACRGRGFEIEVLGLMESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHN 60

Query: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRM 120
           ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQ  QERRM
Sbjct: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQGRQERRM 120

Query: 121 MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 180
           MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR
Sbjct: 121 MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 180

Query: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240
           RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT
Sbjct: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240

Query: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHS 300
           ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGL+HKRARG                    
Sbjct: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARG-------------------- 300

Query: 301 CMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360
                         QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL
Sbjct: 301 --------------QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360

Query: 361 VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 420
           VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP
Sbjct: 361 VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 420

Query: 421 TEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 480
            EETRKKIWLVDSKGLIV+SR ESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV
Sbjct: 421 IEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 480

Query: 481 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYD 540
           GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDP EYD
Sbjct: 481 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD 540

Query: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 600
           GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAA VSQENYDKGLIYPPF
Sbjct: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAHVSQENYDKGLIYPPF 600

Query: 601 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 651
           TNIRKISANIAAKVAAKAYELGLA+RLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 601 TNIRKISANIAAKVAAKAYELGLATRLPRPKDLVKFAESCMYSPRYRSYL 616

BLAST of Lsi08G010730 vs. NCBI nr
Match: XP_011649727.1 (NADP-dependent malic enzyme isoform X1 [Cucumis sativus] >KGN63047.2 hypothetical protein Csa_022109 [Cucumis sativus])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 595/650 (91.54%), Postives = 606/650 (93.23%), Query Frame = 0

Query: 1   MLSFLRHQYLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHN 60
           ML FLRHQYLRNL CRGR FEIE LG M S MKKTMS+GSVH+++EEIDNKFEEAYGEHN
Sbjct: 1   MLFFLRHQYLRNLVCRGRRFEIEALGSMESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHN 60

Query: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRM 120
           ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPA++DQELQERRM
Sbjct: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAVIDQELQERRM 120

Query: 121 MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 180
           MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLP+VYTPTVGEACQKYGS+YR
Sbjct: 121 MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKYGSVYR 180

Query: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240
           RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT
Sbjct: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240

Query: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHS 300
           ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGL+ KRARG                    
Sbjct: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARG-------------------- 300

Query: 301 CMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360
                         QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL
Sbjct: 301 --------------QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360

Query: 361 VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 420
           VFNDDIQGTASVVVAGLLAALKL+GGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP
Sbjct: 361 VFNDDIQGTASVVVAGLLAALKLIGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 420

Query: 421 TEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 480
           TEETRKKIWLVDSKGLIV+SR ESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV
Sbjct: 421 TEETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 480

Query: 481 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYD 540
           GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDP EYD
Sbjct: 481 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYD 540

Query: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 600
           GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF
Sbjct: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 600

Query: 601 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 651
           TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 601 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 616

BLAST of Lsi08G010730 vs. NCBI nr
Match: XP_008445000.2 (PREDICTED: NADP-dependent malic enzyme [Cucumis melo])

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 594/648 (91.67%), Postives = 601/648 (92.75%), Query Frame = 0

Query: 3   SFLRHQYLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHNAT 62
           SFLRHQYLRNL   GR FEIE LG M S MKKTMS+GSVH+E+EEIDNKFEEAYGEHNAT
Sbjct: 4   SFLRHQYLRNLVFIGRRFEIEALGSMESSMKKTMSNGSVHMEMEEIDNKFEEAYGEHNAT 63

Query: 63  EDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRMMH 122
           EDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKER+AHYLRGLLPPALLDQELQERRMMH
Sbjct: 64  EDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERNAHYLRGLLPPALLDQELQERRMMH 123

Query: 123 NLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRP 182
           NLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRP
Sbjct: 124 NLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRP 183

Query: 183 QGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 242
           QGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL
Sbjct: 184 QGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTAL 243

Query: 243 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHSCM 302
           GGIRPSACLPITIDVGTNNEQLLNDEFYIGL+ KRARG                      
Sbjct: 244 GGIRPSACLPITIDVGTNNEQLLNDEFYIGLRQKRARG---------------------- 303

Query: 303 NVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 362
                       QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF
Sbjct: 304 ------------QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVF 363

Query: 363 NDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTE 422
           NDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP E
Sbjct: 364 NDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPIE 423

Query: 423 ETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 482
           ETRKKIWLVDSKGLIV+SR ESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK
Sbjct: 424 ETRKKIWLVDSKGLIVQSRFESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGK 483

Query: 483 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYDGK 542
           TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDP EYDGK
Sbjct: 484 TFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGK 543

Query: 543 VFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 602
            FVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN
Sbjct: 544 AFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTN 603

Query: 603 IRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 651
           IRKISANIAA+VAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL
Sbjct: 604 IRKISANIAAEVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 617

BLAST of Lsi08G010730 vs. NCBI nr
Match: XP_022132027.1 (NADP-dependent malic enzyme [Momordica charantia])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 579/650 (89.08%), Postives = 590/650 (90.77%), Query Frame = 0

Query: 1   MLSFLRHQYLRNLGCRGRGFEIEVLGLMGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHN 60
           ML FLRHQYLR L   GR        LM   MKKTMS+GSVH+E EEIDNKFEEAYGEHN
Sbjct: 1   MLCFLRHQYLRQLAGSGR-------ALMEGAMKKTMSNGSVHMEREEIDNKFEEAYGEHN 60

Query: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRM 120
           ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPA+LDQELQERRM
Sbjct: 61  ATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPAILDQELQERRM 120

Query: 121 MHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 180
           MHNLR Y+VPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR
Sbjct: 121 MHNLRQYDVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 180

Query: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240
           RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT
Sbjct: 181 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 240

Query: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHS 300
           ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGL+HKRARG                    
Sbjct: 241 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLRHKRARG-------------------- 300

Query: 301 CMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360
                         QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL
Sbjct: 301 --------------QEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 360

Query: 361 VFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAP 420
           VFNDDIQGTASVVVAGLLAALKL+GGTL DHTFLFLGAGEAGTGIAELIALEISKQT AP
Sbjct: 361 VFNDDIQGTASVVVAGLLAALKLIGGTLTDHTFLFLGAGEAGTGIAELIALEISKQTKAP 420

Query: 421 TEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGV 480
            EETR+KIWLVDSKGLIVRSR ESLQHFKKPWAHDHESIKDL GAVQAIKPTVLIGTSGV
Sbjct: 421 VEETRRKIWLVDSKGLIVRSRFESLQHFKKPWAHDHESIKDLLGAVQAIKPTVLIGTSGV 480

Query: 481 GKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYD 540
           GKTFTKEVVEAM S NEKPLILALSNPTSQSECTAEEAYTWS+GRAI+ASGSPFDP EYD
Sbjct: 481 GKTFTKEVVEAMESLNEKPLILALSNPTSQSECTAEEAYTWSKGRAIYASGSPFDPVEYD 540

Query: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPF 600
           GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAA+VSQENYDKGLIYPPF
Sbjct: 541 GKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAEVSQENYDKGLIYPPF 600

Query: 601 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSYL 651
           TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYR+YL
Sbjct: 601 TNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRNYL 609

BLAST of Lsi08G010730 vs. NCBI nr
Match: XP_031737340.1 (NADP-dependent malic enzyme isoform X2 [Cucumis sativus])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 573/623 (91.97%), Postives = 584/623 (93.74%), Query Frame = 0

Query: 28  MGSLMKKTMSSGSVHIEIEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 87
           M S MKKTMS+GSVH+++EEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN
Sbjct: 1   MESSMKKTMSNGSVHMDMEEIDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYN 60

Query: 88  KGLAFSEKERDAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 147
           KGLAFSEKERDAHYLRGLLPPA++DQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL
Sbjct: 61  KGLAFSEKERDAHYLRGLLPPAVIDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERL 120

Query: 148 FYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNI 207
           FYKLLIDNVEELLP+VYTPTVGEACQKYGS+YRRPQGLFISLKEKGKILEVLKNWPERNI
Sbjct: 121 FYKLLIDNVEELLPIVYTPTVGEACQKYGSVYRRPQGLFISLKEKGKILEVLKNWPERNI 180

Query: 208 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 267
           QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND
Sbjct: 181 QVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLND 240

Query: 268 EFYIGLKHKRARGQVKHSLAPFLPPDFMISIHSCMNVLINFCFFFLIQEYMELLDEFMYA 327
           EFYIGL+ KRARG                                  QEYMELLDEFMYA
Sbjct: 241 EFYIGLRQKRARG----------------------------------QEYMELLDEFMYA 300

Query: 328 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGT 387
           VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKL+GGT
Sbjct: 301 VKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLIGGT 360

Query: 388 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQH 447
           LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIV+SR ESLQH
Sbjct: 361 LADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVQSRFESLQH 420

Query: 448 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 507
           FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP
Sbjct: 421 FKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNP 480

Query: 508 TSQSECTAEEAYTWSQGRAIFASGSPFDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 567
           TSQSECTAEEAYTWSQGRAIFASGSPFDP EYDGKVFVPGQANNAYIFPGFGLGLIMSGT
Sbjct: 481 TSQSECTAEEAYTWSQGRAIFASGSPFDPVEYDGKVFVPGQANNAYIFPGFGLGLIMSGT 540

Query: 568 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 627
           IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL
Sbjct: 541 IRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRL 589

Query: 628 PRPKDLVKFAESCMYSPRYRSYL 651
           PRPKDLVKFAESCMYSPRYRSYL
Sbjct: 601 PRPKDLVKFAESCMYSPRYRSYL 589

BLAST of Lsi08G010730 vs. TAIR 10
Match: AT5G25880.1 (NADP-malic enzyme 3 )

HSP 1 Score: 937.6 bits (2422), Expect = 5.5e-273
Identity = 467/596 (78.36%), Postives = 514/596 (86.24%), Query Frame = 0

Query: 54  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQ 113
           + YGE +AT DQL+TPW  SVASGY+L+RDP YNKGLAF++KERDAHY+ GLLPP +L Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYITGLLPPVVLSQ 85

Query: 114 ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 173
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 174 KYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 233
           KYGSIYRRPQGL+ISLKEKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIYRRPQGLYISLKEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 234 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPD 293
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLN+EFYIGLK KRA G             
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNNEFYIGLKQKRANG------------- 265

Query: 294 FMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLS 353
                                +EY E L EFM AVK+NYGEKVL+QFEDFANH+AFELLS
Sbjct: 266 ---------------------EEYAEFLQEFMCAVKQNYGEKVLVQFEDFANHHAFELLS 325

Query: 354 RYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEI 413
           +Y SSHLVFNDDIQGTASVV+AGL+AA K++G +LADHTFLFLGAGEAGTGIAELIAL+I
Sbjct: 326 KYCSSHLVFNDDIQGTASVVLAGLIAAQKVLGKSLADHTFLFLGAGEAGTGIAELIALKI 385

Query: 414 SKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTV 473
           SK+TG P +ETRKKIWLVDSKGLIV  R ESLQHFK+PWAHDH+ +K+L  AV AIKPTV
Sbjct: 386 SKETGKPIDETRKKIWLVDSKGLIVSERKESLQHFKQPWAHDHKPVKELLAAVNAIKPTV 445

Query: 474 LIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSP 533
           LIGTSGVGKTFTKEVVEAMA+ NEKPLILALSNPTSQ+ECTAEEAYTW++GRAIFASGSP
Sbjct: 446 LIGTSGVGKTFTKEVVEAMATLNEKPLILALSNPTSQAECTAEEAYTWTKGRAIFASGSP 505

Query: 534 FDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDK 593
           FDP +YDGK F PGQANN YIFPG GLGLIMSG IRV DDMLLAASEALA+QV++EN+  
Sbjct: 506 FDPVQYDGKKFTPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAASEALASQVTEENFAN 565

Query: 594 GLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 650
           GLIYPPF NIRKISANIAA V AK YELGLAS LPRPKDLVK AESCMYSP YR++
Sbjct: 566 GLIYPPFANIRKISANIAASVGAKTYELGLASNLPRPKDLVKMAESCMYSPVYRNF 587

BLAST of Lsi08G010730 vs. TAIR 10
Match: AT5G11670.1 (NADP-malic enzyme 2 )

HSP 1 Score: 929.1 bits (2400), Expect = 2.0e-270
Identity = 465/596 (78.02%), Postives = 514/596 (86.24%), Query Frame = 0

Query: 54  EAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQ 113
           + YGE +AT DQL+TPW  SVASGY+L+RDP YNKGLAF++KERDAHYL GLLPP +L Q
Sbjct: 26  DVYGEDSATLDQLVTPWVTSVASGYTLMRDPRYNKGLAFTDKERDAHYLTGLLPPVILSQ 85

Query: 114 ELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 173
           ++QER++MHNLR Y VPL RY+A+MDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ
Sbjct: 86  DVQERKVMHNLRQYTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQ 145

Query: 174 KYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPV 233
           KYGSI+R+PQGL+ISL EKGKILEVLKNWP+R IQVIVVTDGERILGLGDLGCQGMGIPV
Sbjct: 146 KYGSIFRKPQGLYISLNEKGKILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPV 205

Query: 234 GKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPD 293
           GKLSLYTALGGIRPSACLPITIDVGTNNE+LLNDEFYIGLK +RA G             
Sbjct: 206 GKLSLYTALGGIRPSACLPITIDVGTNNEKLLNDEFYIGLKQRRATG------------- 265

Query: 294 FMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLS 353
                                QEY E L EFM AVK+NYGEKVL+QFEDFANHNAF+LLS
Sbjct: 266 ---------------------QEYAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLS 325

Query: 354 RYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAELIALEI 413
           +YS SHLVFNDDIQGTASVV+AGL+AA K++G  LADHTFLFLGAGEAGTGIAELIAL+I
Sbjct: 326 KYSDSHLVFNDDIQGTASVVLAGLIAAQKVLGKKLADHTFLFLGAGEAGTGIAELIALKI 385

Query: 414 SKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQAIKPTV 473
           SK+TGAP  ETRKKIWLVDSKGLIV SR ESLQHFK+PWAH+H+ +KDL GAV AIKPTV
Sbjct: 386 SKETGAPITETRKKIWLVDSKGLIVSSRKESLQHFKQPWAHEHKPVKDLIGAVNAIKPTV 445

Query: 474 LIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAIFASGSP 533
           LIGTSGVG+TFTKEVVEAMA+ NEKPLILALSNPTSQ+ECTAE+AYTW++GRAIF SGSP
Sbjct: 446 LIGTSGVGQTFTKEVVEAMATNNEKPLILALSNPTSQAECTAEQAYTWTKGRAIFGSGSP 505

Query: 534 FDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVSQENYDK 593
           FDP  YDGK ++PGQANN YIFPG GLGLIMSG IRV DDMLLAASEALAAQV++E+Y  
Sbjct: 506 FDPVVYDGKTYLPGQANNCYIFPGLGLGLIMSGAIRVRDDMLLAASEALAAQVTEEHYAN 565

Query: 594 GLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYRSY 650
           GLIYPPF+NIR+ISANIAA VAAK Y+LGLAS LPR KDLVKFAES MYSP YR+Y
Sbjct: 566 GLIYPPFSNIREISANIAACVAAKTYDLGLASNLPRAKDLVKFAESSMYSPVYRNY 587

BLAST of Lsi08G010730 vs. TAIR 10
Match: AT1G79750.1 (NADP-malic enzyme 4 )

HSP 1 Score: 916.8 bits (2368), Expect = 1.0e-266
Identity = 459/629 (72.97%), Postives = 527/629 (83.78%), Query Frame = 0

Query: 29  GSLMKKTMSSGSVHIEIEEID--------NKFEEAYGEHNATEDQLITPWTVSVASGYSL 88
           GS++ +T ++ S  +E    D           ++ YGE  ATED  ITPW++SVASGY+L
Sbjct: 51  GSVLIETTATSSSSLETSAADIVPKSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTL 110

Query: 89  LRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRMMHNLRNYEVPLHRYIAMMDL 148
           LRDP +NKGLAFS +ERDAHYLRGLLPP ++ Q+LQ +++MH LR Y+VPL +Y+AMMDL
Sbjct: 111 LRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDL 170

Query: 149 QERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLFISLKEKGKILEVLK 208
           QE NERLFYKLLID+VEELLPV+YTPTVGEACQKYGSI+ RPQGLFISLKEKGKI EVL+
Sbjct: 171 QETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLR 230

Query: 209 NWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTN 268
           NWPE+NIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLP+TIDVGTN
Sbjct: 231 NWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTN 290

Query: 269 NEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHSCMNVLINFCFFFLIQEYMEL 328
           NE+LLNDEFYIGL+ +RA G                                  +EY EL
Sbjct: 291 NEKLLNDEFYIGLRQRRATG----------------------------------EEYSEL 350

Query: 329 LDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHLVFNDDIQGTASVVVAGLLAA 388
           + EFM AVK+NYGEKV+IQFEDFANHNAF+LL++Y ++HLVFNDDIQGTASVV+AGL+AA
Sbjct: 351 MHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAA 410

Query: 389 LKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQTGAPTEETRKKIWLVDSKGLIVRS 448
           L+ VGG+L+DH FLFLGAGEAGTGIAELIALEISK++  P EE RK IWLVDSKGLIV S
Sbjct: 411 LRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSS 470

Query: 449 RLESLQHFKKPWAHDHESIKDLYGAVQAIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPL 508
           R ES+QHFKKPWAHDHE I++L  AV+AIKPTVLIGTSGVG+TFT++VVE MA  NEKP+
Sbjct: 471 RKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPI 530

Query: 509 ILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFDPFEYDGKVFVPGQANNAYIFPGFGL 568
           IL+LSNPTSQSECTAEEAYTWSQGRAIFASGSPF P EY+GK FVPGQANNAYIFPGFGL
Sbjct: 531 ILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGL 590

Query: 569 GLIMSGTIRVHDDMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYE 628
           GLIMSGTIRVHDDMLLAASEALA ++ +E+Y+KG+IYPPF NIRKISA IAAKVAAKAYE
Sbjct: 591 GLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYE 645

Query: 629 LGLASRLPRPKDLVKFAESCMYSPRYRSY 650
           LGLA+RLP+PK+L + AES MYSP YRSY
Sbjct: 651 LGLATRLPQPKELEQCAESSMYSPSYRSY 645

BLAST of Lsi08G010730 vs. TAIR 10
Match: AT2G19900.1 (NADP-malic enzyme 1 )

HSP 1 Score: 911.8 bits (2355), Expect = 3.2e-265
Identity = 451/602 (74.92%), Postives = 517/602 (85.88%), Query Frame = 0

Query: 48  IDNKFEEAYGEHNATEDQLITPWTVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLP 107
           +D    + YGE +AT +  ITPW++SV+SGYSLLRDP YNKGLAF+EKERD HYLRGLLP
Sbjct: 13  VDGGVVDVYGEDSATIEHNITPWSLSVSSGYSLLRDPRYNKGLAFTEKERDTHYLRGLLP 72

Query: 108 PALLDQELQERRMMHNLRNYEVPLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPT 167
           P +LDQ+LQE+R+++N+R Y+ PL +Y+A+ +LQERNERLFYKLLIDNVEELLP+VYTPT
Sbjct: 73  PVVLDQKLQEKRLLNNIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPT 132

Query: 168 VGEACQKYGSIYRRPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQ 227
           VGEACQK+GSI+RRPQGLFISLK+KGKIL+VLKNWPERNIQVIVVTDGERILGLGDLGCQ
Sbjct: 133 VGEACQKFGSIFRRPQGLFISLKDKGKILDVLKNWPERNIQVIVVTDGERILGLGDLGCQ 192

Query: 228 GMGIPVGKLSLYTALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLA 287
           GMGIPVGKL+LY+ALGG+RPSACLP+TIDVGTNNE+LLNDEFYIGL+ KRA G       
Sbjct: 193 GMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQKRATG------- 252

Query: 288 PFLPPDFMISIHSCMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHN 347
                                      QEY ELL+EFM AVK+NYGEKVLIQFEDFANHN
Sbjct: 253 ---------------------------QEYSELLNEFMSAVKQNYGEKVLIQFEDFANHN 312

Query: 348 AFELLSRYSSSHLVFNDDIQGTASVVVAGLLAALKLVGGTLADHTFLFLGAGEAGTGIAE 407
           AFELL++YS +HLVFNDDIQGTASVV+AGL++A KL    LA+HTFLFLGAGEAGTGIAE
Sbjct: 313 AFELLAKYSDTHLVFNDDIQGTASVVLAGLVSAQKLTNSPLAEHTFLFLGAGEAGTGIAE 372

Query: 408 LIALEISKQTGAPTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAHDHESIKDLYGAVQ 467
           LIAL +SKQ  A  EE+RKKIWLVDSKGLIV SR +SLQ FKKPWAH+HE +KDL GA++
Sbjct: 373 LIALYMSKQMNASVEESRKKIWLVDSKGLIVNSRKDSLQDFKKPWAHEHEPVKDLLGAIK 432

Query: 468 AIKPTVLIGTSGVGKTFTKEVVEAMASFNEKPLILALSNPTSQSECTAEEAYTWSQGRAI 527
           AIKPTVLIG+SGVG++FTKEV+EAM+S NE+PLI+ALSNPT+QSECTAEEAYTWS+GRAI
Sbjct: 433 AIKPTVLIGSSGVGRSFTKEVIEAMSSINERPLIMALSNPTTQSECTAEEAYTWSKGRAI 492

Query: 528 FASGSPFDPFEYDGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAAQVS 587
           FASGSPFDP EY+GKVFV  QANNAYIFPGFGLGL++SG IRVHDDMLLAA+EALA QVS
Sbjct: 493 FASGSPFDPVEYEGKVFVSTQANNAYIFPGFGLGLVISGAIRVHDDMLLAAAEALAGQVS 552

Query: 588 QENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGLASRLPRPKDLVKFAESCMYSPRYR 647
           +ENY+KG+IYP F++IRKISA IAA VA KAYELGLA RLPRPKD+VK AES MYSP YR
Sbjct: 553 KENYEKGMIYPSFSSIRKISAQIAANVATKAYELGLAGRLPRPKDIVKCAESSMYSPTYR 580

Query: 648 SY 650
            Y
Sbjct: 613 LY 580

BLAST of Lsi08G010730 vs. TAIR 10
Match: AT2G13560.1 (NAD-dependent malic enzyme 1 )

HSP 1 Score: 414.5 bits (1064), Expect = 1.6e-115
Identity = 242/612 (39.54%), Postives = 353/612 (57.68%), Query Frame = 0

Query: 71  TVSVASGYSLLRDPIYNKGLAFSEKERDAHYLRGLLPPALLDQELQERRMMHNLRNYEV- 130
           T+    G  +L DP +NKG AF+  ER+   LRGLLPP ++D E Q  R M +L+  E  
Sbjct: 40  TIVHKQGLDILHDPWFNKGTAFTMTERNRLDLRGLLPPNVMDSEQQIFRFMTDLKRLEEQ 99

Query: 131 ---------PLHRYIAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYR 190
                     L ++  +  L +RNE ++YK+LI+N+EE  P+VYTPTVG  CQ Y  ++R
Sbjct: 100 ARDGPSDPNALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFR 159

Query: 191 RPQGLFISLKEKGKILEVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYT 250
           RP+G++ S +++G+++ ++ NWP   + +IVVTDG RILGLGDLG  G+GI VGKL LY 
Sbjct: 160 RPRGMYFSAEDRGEMMSMVYNWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYV 219

Query: 251 ALGGIRPSACLPITIDVGTNNEQLLNDEFYIGLKHKRARGQVKHSLAPFLPPDFMISIHS 310
           A  GI P   LP+ IDVGTNNE+L ND  Y+GL+ +R                       
Sbjct: 220 AAAGINPQRVLPVMIDVGTNNEKLRNDPMYLGLQQRRLED-------------------- 279

Query: 311 CMNVLINFCFFFLIQEYMELLDEFMYAVKKNYGEKVLIQFEDFANHNAFELLSRYSSSHL 370
                          +Y++++DEFM AV   +   V++QFEDF +  AF+LL RY  ++ 
Sbjct: 280 --------------DDYIDVIDEFMEAVYTRW-PHVIVQFEDFQSKWAFKLLQRYRCTYR 339

Query: 371 VFNDDIQGTASVVVAGLLAALKLVGGTLADH---TFLFLGAGEAGTGIAELIALEISKQT 430
           +FNDD+QGTA V +AGLL A++  G  + D      +  GAG AG G+       +++  
Sbjct: 340 MFNDDVQGTAGVAIAGLLGAVRAQGRPMIDFPKMKIVVAGAGSAGIGVLNAARKTMARML 399

Query: 431 G---APTEETRKKIWLVDSKGLIVRSRLESLQHFKKPWAH-----DHESIKD---LYGAV 490
           G      +  + + W+VD++GLI   R E++    +P+A      + + +K+   L   V
Sbjct: 400 GNTETAFDSAQSQFWVVDAQGLITEGR-ENIDPEAQPFARKTKEMERQGLKEGATLVEVV 459

Query: 491 QAIKPTVLIGTSGVGKTFTKEVVEAM-ASFNEKPLILALSNPTSQSECTAEEAYTWSQGR 550
           + +KP VL+G S VG  F+KEV+EAM  S + +P I A+SNPT  +ECT ++A++     
Sbjct: 460 REVKPDVLLGLSAVGGLFSKEVLEAMKGSTSTRPAIFAMSNPTKNAECTPQDAFSILGEN 519

Query: 551 AIFASGSPFDPFEY-DGKVFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAA 610
            IFASGSPF   E+ +G V    Q NN Y+FPG GLG ++SG   V D ML AASE LAA
Sbjct: 520 MIFASGSPFKNVEFGNGHVGHCNQGNNMYLFPGIGLGTLLSGAPIVSDGMLQAASECLAA 579

Query: 611 QVSQENYDKGLIYPPFTNIRKISANIAAKVAAKAYELGL--------ASRLPR--PKDLV 647
            +S+E   +G+IYPP + IR I+  IAA V  +A E  L        A  + +   + L+
Sbjct: 580 YMSEEEVLEGIIYPPISRIRDITKRIAAAVIKEAIEEDLVEGYREMDAREIQKLDEEGLM 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P126287.5e-28382.05NADP-dependent malic enzyme OS=Phaseolus vulgaris OX=3885 GN=ME1 PE=2 SV=1[more]
P364441.2e-27277.39NADP-dependent malic enzyme, chloroplastic OS=Flaveria pringlei OX=4226 GN=MODA ... [more]
Q9XGZ07.7e-27278.36NADP-dependent malic enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME3 PE=1 S... [more]
P516151.3e-27178.22NADP-dependent malic enzyme OS=Vitis vinifera OX=29760 PE=2 SV=1[more]
Q9LYG32.7e-26978.02NADP-dependent malic enzyme 2 OS=Arabidopsis thaliana OX=3702 GN=NADP-ME2 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A1S3BB740.0e+0091.67Malic enzyme OS=Cucumis melo OX=3656 GN=LOC103488170 PE=3 SV=1[more]
A0A6J1BSP90.0e+0089.08Malic enzyme OS=Momordica charantia OX=3673 GN=LOC111004998 PE=3 SV=1[more]
A0A0A0LLH90.0e+0091.97Malic enzyme OS=Cucumis sativus OX=3659 GN=Csa_2G373430 PE=3 SV=1[more]
A0A5A7VH340.0e+0092.63Malic enzyme OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003800 P... [more]
A0A6J1KT370.0e+0089.09Malic enzyme OS=Cucurbita maxima OX=3661 GN=LOC111496169 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038884200.10.0e+0092.46NADP-dependent malic enzyme [Benincasa hispida][more]
XP_011649727.10.0e+0091.54NADP-dependent malic enzyme isoform X1 [Cucumis sativus] >KGN63047.2 hypothetica... [more]
XP_008445000.20.0e+0091.67PREDICTED: NADP-dependent malic enzyme [Cucumis melo][more]
XP_022132027.10.0e+0089.08NADP-dependent malic enzyme [Momordica charantia][more]
XP_031737340.10.0e+0091.97NADP-dependent malic enzyme isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT5G25880.15.5e-27378.36NADP-malic enzyme 3 [more]
AT5G11670.12.0e-27078.02NADP-malic enzyme 2 [more]
AT1G79750.11.0e-26672.97NADP-malic enzyme 4 [more]
AT2G19900.13.2e-26574.92NADP-malic enzyme 1 [more]
AT2G13560.11.6e-11539.54NAD-dependent malic enzyme 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 45..65
NoneNo IPR availableGENE3D3.40.50.720coord: 364..650
e-value: 3.3E-122
score: 409.6
NoneNo IPR availablePANTHERPTHR23406:SF65MALIC ENZYMEcoord: 315..649
NoneNo IPR availablePANTHERPTHR23406:SF65MALIC ENZYMEcoord: 34..298
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 34..298
NoneNo IPR availablePANTHERPTHR23406MALIC ENZYME-RELATEDcoord: 315..649
NoneNo IPR availableCDDcd05312NAD_bind_1_malic_enzcoord: 366..644
e-value: 3.97634E-146
score: 423.498
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 315..365
NoneNo IPR availableSUPERFAMILY53223Aminoacid dehydrogenase-like, N-terminal domaincoord: 55..284
IPR001891Malic oxidoreductasePRINTSPR00072MALOXRDTASEcoord: 496..512
score: 67.06
coord: 147..171
score: 69.8
coord: 243..265
score: 67.72
coord: 207..236
score: 82.75
IPR001891Malic oxidoreductasePIRSFPIRSF000106MEcoord: 314..650
e-value: 4.0E-133
score: 442.9
coord: 40..288
e-value: 8.9E-93
score: 309.7
IPR012302Malic enzyme, NAD-bindingSMARTSM00919Malic_M_2coord: 366..619
e-value: 1.0E-114
score: 397.1
IPR012302Malic enzyme, NAD-bindingPFAMPF03949Malic_Mcoord: 366..618
e-value: 9.0E-97
score: 323.4
IPR012301Malic enzyme, N-terminal domainSMARTSM01274malic_2coord: 141..356
e-value: 2.5E-86
score: 302.8
IPR012301Malic enzyme, N-terminal domainPFAMPF00390maliccoord: 141..285
e-value: 1.1E-62
score: 211.1
coord: 315..355
e-value: 1.5E-9
score: 37.9
IPR037062Malic enzyme, N-terminal domain superfamilyGENE3D3.40.50.10380coord: 75..363
e-value: 1.9E-119
score: 399.4
IPR015884Malic enzyme, conserved sitePROSITEPS00331MALIC_ENZYMEScoord: 362..378
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 366..649

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G010730.1Lsi08G010730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006108 malate metabolic process
molecular_function GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0004470 malic enzyme activity