Lsi08G010660 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G010660
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPolynucleotide adenylyltransferase
Locationchr08: 19261865 .. 19263212 (+)
RNA-Seq ExpressionLsi08G010660
SyntenyLsi08G010660
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTAAGAGAAGTGGCATTCTAGGGATAGTGAGTCGGGTATTTGCAGGGCAATGATAGCAGGATGGATCCAGATTTGGCATTGGAAAATGGTTCTGTTGTGACTGGAAGTGTGTCTCAAAATGGTCAATCTAAGGAGTTGGAGGTTTGAATCTAATTGTTAAATTACTTTCATTTTATATATATTTACACGATACTCTTATAACTGATTATGGTGCTTCCACCAAGGAATCTAGGATGTGAGGAGGTTGAGATTTTAATTGATATCAATCAGAAGAATAGAAGATATAAACACATTTCTGGTTTGATACCTTAATAATATGCATATATTTATTTTGCAAAAGAAGCAACTTTTGTTGTTTTGTTCCATTTCTCTTCACCATGTGTCTTTCCTTCTCTCTTTCCGAACCATAATTATTGGTCTATTTTCCTTTTGCATTTTAATTATCATTTTGTAATGCCAAGTTTTCGCACAGGCAATCTTCTGACTGGTGCACATATGGTGGCAGGTCCTGCACCACTATACGTGTTATGTTACCAGTAACTGTGCTAAAGAACCCATTTCTGCTCAAGTTTTTCCTGCATTACCTTTTTTTCTCATGAAAATCTTTGCCTAATTAGTAATTTTCTTTTTGAACTTTATTTTATAGCTGAAGATCAGCGATCAGGCAGTCCCAGAATGGAGTTAAATCAGAATCTAAGCAAAATTCTGGTAAAAAAGAGTCTTGGGGGATCTGGCCACAAGGCATGTGTATTTAGATTCAATGCAATTTCTATTGGATAAAGAAATTATTTTTCCGAATCTGGCGAAGGGCATGTGCTTTTTCCCGTTCTAGTACTTAAGTTGACTGATTATTATGTTTATTTTGATCATCTTCTCTTTATGTTGCTTGTTTGGCTCTTAGCATCCTTTGAACAGACTGAATCTGAACCTCAAGGCATGACATGTGGAGGGGAAGTACTGGATGCTGGATGGGTTGGGAGGTCGGGAGGACCAGCATTTGGTAAGTATCACCTCAATTACTGGCTTTGATTATTGATGTAGATGGTGTTCATCATGTATGAACCTGCGGACTTGGGGAGGAGTCGCGTGGCATGGTGGCGTGGCATACGGTTCAGCTCCAGTATGCTGTTTATTGTCCTTGAACATGGGAATCCGAAGGAGTGCTGTTGGGGAAAAAACCTTAAATAAGTGCATGCGATGCTTTGAAGAATCTGGTGCATTTCATGTTAGATGCCTGGTGATGGAACTGTCACAGCTTTCCATTTTTAGAAGATGGGAGATTCTTAGTGAAGGAACTGCTCATGGGGTCGTTTTCGGGGCGAATCTAGTCTACACTTATTGA

mRNA sequence

ATGGCAGGCAATGATAGCAGGATGGATCCAGATTTGGCATTGGAAAATGGTTCTGTTGTGACTGGAAGTGTGTCTCAAAATGGTCAATCTAAGGAGTTGGAGACTGAATCTGAACCTCAAGGCATGACATGTGGAGGGGAAGTACTGGATGCTGGATGGGTTGGGAGGTCGGGAGGACCAGCATTTGGTAAATGGTGTTCATCATGTATGAACCTGCGGACTTGGGGAGGAGTCGCGTGGCATGGTGGCGTGGCATACGGTTCAGCTCCAGTATGCTGTTTATTGTCCTTGAACATGGGAATCCGAAGGAGTGCTGTTGGGGAAAAAACCTTAAATAAGTGCATGCGATGCTTTGAAGAATCTGGTGCATTTCATGTTAGATGCCTGGTGATGGAACTGTCACAGCTTTCCATTTTTAGAAGATGGGAGATTCTTAGTGAAGGAACTGCTCATGGGGTCGTTTTCGGGGCGAATCTAGTCTACACTTATTGA

Coding sequence (CDS)

ATGGCAGGCAATGATAGCAGGATGGATCCAGATTTGGCATTGGAAAATGGTTCTGTTGTGACTGGAAGTGTGTCTCAAAATGGTCAATCTAAGGAGTTGGAGACTGAATCTGAACCTCAAGGCATGACATGTGGAGGGGAAGTACTGGATGCTGGATGGGTTGGGAGGTCGGGAGGACCAGCATTTGGTAAATGGTGTTCATCATGTATGAACCTGCGGACTTGGGGAGGAGTCGCGTGGCATGGTGGCGTGGCATACGGTTCAGCTCCAGTATGCTGTTTATTGTCCTTGAACATGGGAATCCGAAGGAGTGCTGTTGGGGAAAAAACCTTAAATAAGTGCATGCGATGCTTTGAAGAATCTGGTGCATTTCATGTTAGATGCCTGGTGATGGAACTGTCACAGCTTTCCATTTTTAGAAGATGGGAGATTCTTAGTGAAGGAACTGCTCATGGGGTCGTTTTCGGGGCGAATCTAGTCTACACTTATTGA

Protein sequence

MAGNDSRMDPDLALENGSVVTGSVSQNGQSKELETESEPQGMTCGGEVLDAGWVGRSGGPAFGKWCSSCMNLRTWGGVAWHGGVAYGSAPVCCLLSLNMGIRRSAVGEKTLNKCMRCFEESGAFHVRCLVMELSQLSIFRRWEILSEGTAHGVVFGANLVYTY
Homology
BLAST of Lsi08G010660 vs. ExPASy TrEMBL
Match: A0A5A7VGN2 (Polynucleotide adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003840 PE=3 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 3.0e-26
Identity = 88/210 (41.90%), Postives = 96/210 (45.71%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQ--------------------------------- 62
           GNDSR DPDLAL+NGSVVTG VSQNG                                  
Sbjct: 715 GNDSRTDPDLALDNGSVVTGRVSQNGNLLTGAHVVAGSPPLCVFCYHRRSAIRQSPNGVN 774

Query: 63  ---SKELETES---------------EPQGMTCGGEVLDAGWVGRSGGPAFGKWCSSCMN 122
              SK L  +S                 + M C GEVLDAGWV RSG             
Sbjct: 775 QNLSKILVKKSLGGSGYKHPLNRLNLNLEDMACEGEVLDAGWVVRSGADHH--------- 834

Query: 123 LRTWGGVAWHGGVAYGSAPVCCLLSLNMGIRRSAVGEKTLNKCMRCFEESGAFHVRCLVM 162
                                 LL   MGI+ S+VGEKTL +CM+CFEESGA H RCL  
Sbjct: 835 ----------------------LLQYAMGIQSSSVGEKTLKRCMQCFEESGALHFRCLAF 893

BLAST of Lsi08G010660 vs. ExPASy TrEMBL
Match: A0A0A0LLH3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373390 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.1e-15
Identity = 45/85 (52.94%), Postives = 55/85 (64.71%), Query Frame = 0

Query: 78  VAWHGGVAYGSAPVCCLLSLNMGIRRSAVGEKTLNKCMRCFEESGAFHVRCLVMELSQLS 137
           +AW  G+ + S  +   + L+ G  +     K L  CM+CFEESGAFH RCL +E SQL 
Sbjct: 1   MAWWHGIWFSSNML--FIVLDHGNPKQQCWGKNLKDCMQCFEESGAFHFRCLALEPSQLP 60

Query: 138 IFRRWEILSEGTAHGVVFGANLVYT 163
           IFRRWEIL EGTAH VV G N +YT
Sbjct: 61  IFRRWEILCEGTAHAVVSGVNAIYT 83

BLAST of Lsi08G010660 vs. ExPASy TrEMBL
Match: A0A1S3BCI7 (Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103488167 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.2e-05
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQSKELETESEPQGMT 44
           GNDSR DPDLAL+NGSVVTG VSQNGQSK LE +   Q +T
Sbjct: 742 GNDSRTDPDLALDNGSVVTGRVSQNGQSKVLEPKISNQAVT 782

BLAST of Lsi08G010660 vs. ExPASy TrEMBL
Match: A0A0A0LP30 (Polynucleotide adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G373380 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.2e-05
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQSKELETESEPQGMT 44
           GNDSR DPDLAL+NGSVVTG VSQNGQSK LE +   Q +T
Sbjct: 718 GNDSRTDPDLALDNGSVVTGRVSQNGQSKVLEPKISNQAVT 758

BLAST of Lsi08G010660 vs. ExPASy TrEMBL
Match: A0A1S3BBN8 (Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103488167 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.2e-05
Identity = 30/41 (73.17%), Postives = 33/41 (80.49%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQSKELETESEPQGMT 44
           GNDSR DPDLAL+NGSVVTG VSQNGQSK LE +   Q +T
Sbjct: 714 GNDSRTDPDLALDNGSVVTGRVSQNGQSKVLEPKISNQAVT 754

BLAST of Lsi08G010660 vs. NCBI nr
Match: KAA0065055.1 (nuclear poly(A) polymerase 4 isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 128.3 bits (321), Expect = 6.2e-26
Identity = 88/210 (41.90%), Postives = 96/210 (45.71%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQ--------------------------------- 62
           GNDSR DPDLAL+NGSVVTG VSQNG                                  
Sbjct: 715 GNDSRTDPDLALDNGSVVTGRVSQNGNLLTGAHVVAGSPPLCVFCYHRRSAIRQSPNGVN 774

Query: 63  ---SKELETES---------------EPQGMTCGGEVLDAGWVGRSGGPAFGKWCSSCMN 122
              SK L  +S                 + M C GEVLDAGWV RSG             
Sbjct: 775 QNLSKILVKKSLGGSGYKHPLNRLNLNLEDMACEGEVLDAGWVVRSGADHH--------- 834

Query: 123 LRTWGGVAWHGGVAYGSAPVCCLLSLNMGIRRSAVGEKTLNKCMRCFEESGAFHVRCLVM 162
                                 LL   MGI+ S+VGEKTL +CM+CFEESGA H RCL  
Sbjct: 835 ----------------------LLQYAMGIQSSSVGEKTLKRCMQCFEESGALHFRCLAF 893

BLAST of Lsi08G010660 vs. NCBI nr
Match: KGN62795.1 (hypothetical protein Csa_022232 [Cucumis sativus])

HSP 1 Score: 91.7 bits (226), Expect = 6.5e-15
Identity = 45/85 (52.94%), Postives = 55/85 (64.71%), Query Frame = 0

Query: 78  VAWHGGVAYGSAPVCCLLSLNMGIRRSAVGEKTLNKCMRCFEESGAFHVRCLVMELSQLS 137
           +AW  G+ + S  +   + L+ G  +     K L  CM+CFEESGAFH RCL +E SQL 
Sbjct: 1   MAWWHGIWFSSNML--FIVLDHGNPKQQCWGKNLKDCMQCFEESGAFHFRCLALEPSQLP 60

Query: 138 IFRRWEILSEGTAHGVVFGANLVYT 163
           IFRRWEIL EGTAH VV G N +YT
Sbjct: 61  IFRRWEILCEGTAHAVVSGVNAIYT 83

BLAST of Lsi08G010660 vs. NCBI nr
Match: XP_038884464.1 (nuclear poly(A) polymerase 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 63.2 bits (152), Expect = 2.5e-06
Identity = 32/41 (78.05%), Postives = 34/41 (82.93%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQSKELETESEPQGMT 44
           GNDSR DPDLALENGSVVTG VSQNGQSKELE +   Q +T
Sbjct: 748 GNDSRTDPDLALENGSVVTGRVSQNGQSKELEPKISDQAVT 788

BLAST of Lsi08G010660 vs. NCBI nr
Match: XP_038884467.1 (nuclear poly(A) polymerase 4-like isoform X3 [Benincasa hispida])

HSP 1 Score: 63.2 bits (152), Expect = 2.5e-06
Identity = 32/41 (78.05%), Postives = 34/41 (82.93%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQSKELETESEPQGMT 44
           GNDSR DPDLALENGSVVTG VSQNGQSKELE +   Q +T
Sbjct: 718 GNDSRTDPDLALENGSVVTGRVSQNGQSKELEPKISDQAVT 758

BLAST of Lsi08G010660 vs. NCBI nr
Match: XP_038884466.1 (nuclear poly(A) polymerase 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 60.5 bits (145), Expect = 1.6e-05
Identity = 30/32 (93.75%), Postives = 30/32 (93.75%), Query Frame = 0

Query: 3   GNDSRMDPDLALENGSVVTGSVSQNGQSKELE 35
           GNDSR DPDLALENGSVVTG VSQNGQSKELE
Sbjct: 748 GNDSRTDPDLALENGSVVTGRVSQNGQSKELE 779

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7VGN23.0e-2641.90Polynucleotide adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A0A0LLH33.1e-1552.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G373390 PE=4 SV=1[more]
A0A1S3BCI72.2e-0573.17Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103488167 PE=3 ... [more]
A0A0A0LP302.2e-0573.17Polynucleotide adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G373380 PE... [more]
A0A1S3BBN82.2e-0573.17Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103488167 PE=3 ... [more]
Match NameE-valueIdentityDescription
KAA0065055.16.2e-2641.90nuclear poly(A) polymerase 4 isoform X3 [Cucumis melo var. makuwa][more]
KGN62795.16.5e-1552.94hypothetical protein Csa_022232 [Cucumis sativus][more]
XP_038884464.12.5e-0678.05nuclear poly(A) polymerase 4-like isoform X1 [Benincasa hispida][more]
XP_038884467.12.5e-0678.05nuclear poly(A) polymerase 4-like isoform X3 [Benincasa hispida][more]
XP_038884466.11.6e-0593.75nuclear poly(A) polymerase 4-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..37
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..41

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G010660.1Lsi08G010660.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
biological_process GO:0031123 RNA 3'-end processing
biological_process GO:0043631 RNA polyadenylation
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0004652 polynucleotide adenylyltransferase activity
molecular_function GO:0003723 RNA binding