Lsi08G010570 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G010570
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPoly [ADP-ribose] polymerase
Locationchr08: 19174981 .. 19177462 (+)
RNA-Seq ExpressionLsi08G010570
SyntenyLsi08G010570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAAAGACAAATAATAATAATAATAATAGAAGTCGCTAACTTAATTCCCAAGTAAGTCGATTTCAGCGGGGGAAGCTTCCGTGAATCGGCGCTTCACGTTCACAAGCATCCGTCAAATCGGCGATTTAGTCAATTACCCAAATACCCTCCTCTCCTTAAACTTTTTATTGCCGATAACCGACTTTGACTTAGCCGATTCGTAGGGCTATATGTGTAATTTGGTCTTATTTGGTGACTGGAAACGGATACATATGCCGGCACGGCCCTGTTTCTTTGCCTTGATTCTACTGGTTCTTTCGAAGCGAATTTCAGCGCCGGCGGCTTTCGATCACAGAGACTAGTAGAATCCATGGCCTCCAAGTTACTTATGCATAAGCAATTGAGGAAGAAGATGTTGGGATCAATTGAAGTTCGATCTCCTCTCAGAAACCCTACCAATTCTTCCTCCTCCTCTTCCCATCACTTCTCCGATTCTCCCCAGTCTCTGATTCAGAATTACTCCAATTTCAATACAAGTGGTTTACCTTCACGTTTTATGTTCTATTCGGACAGTTCGTGGGTTGATTTCCCTACCCAAGTGCTTGAAATCTTGCGAACGGGTTTCTTCGACCGGAAGCCGGCGATGGAATTGCAAATTGATGGATCGAAGTACGTATTTGATTTGTACAGGATGTTGCAGATTGATTTAGAAACTGGTAGACAGAGATCAGTTGCTTGGATCGATGAAAACGGTAAGTGTTTCTTCCCCAAAGTTTTTATTAGGGAAGAAAGTGTGAATACCTCCCGGACTTCTAGTAGTCCGAAAATTGAAATTGAGATTACGATTGATGGGAAATCCGGGAAGAGAAAGAGGGAGGCGATGGAAGAAAATGAAGTCAGTTCCAGTAATGAACATGTGCAAGTGAAGGCATCGAAAATACCGCGTGTAGTTGCGAATGATTCAGAAACTTCTGTATGGCCAAAGGCGAAGGCGCTGAATGAAGGGGATAGTGCGTACTCGTTAGTAAGCAATTTCTTTCTCCCTTCAATGAAAAAGGTTGATCCTAGTTTTTCAATCTCTGCAATTCATCAGTGTACCCGTACTGGGCCACTGGAAAAAGCTCGTCTCGATGTGTTTCTGAAGCAGAATGAGATCACAACGGCTGCTCGTGGTGTGTCTAATATGGTTTATGCATGGTATGGAGCTTCTTCAAAAACTTTGGCCGGAATTTTGGCTCATGGATTTGGGGAGCCAGTTCAGGTTCCTTCCTCTGATACCACCCATGGCATTGGAGTTTATTTATCTCCATTGGGATTGCCCTATTTAAGGTAATTTAACTTCTGTTCAAGTTTAGGTACGTTTTAAGTTTAAGTTTATGATTATGATTTCTCACCATTCTCAAACTGCATTCAACTGTGTTTCCCAGTTCTAAGCTATTGGAGGCCGATGGCAATGGAGTTAAACATATGGTTTTATGTCGAGTTATATTGGGTAAGATTGAAAAAGTTGAAGCAGGATCTCAACAATCTCATCCTTCGAGTACAGCCTTTGACACTGGTGTGGATGATCCAACATTTCCAACACGGTATATTGTATGGTGTAGCAATATGAACAGACACATTTTACCAGAATACATAGTGAGCTTTAAATCAACAAATTGTTTGCCGGGTAATTATTATTTTTTATTTGGATAATTTATTTTATCCTCTCTCCCATCTCTTTATTATTATTTTTTTCTTTCTGGCTGATTCTTTTTTCAGGTAAGTTGAGAGAATCGACTGATACGAAGTATTCCCTAGTGAAATTACTTTCGAAGATGAAGAATTCCCTGCCTACTTCCAAAGTTCAAGAGGTTTCAACATTGTTTCAGAAATTTAAGGTAACTCTTTCCTTTGTAATTTTTAAAAGATGAAAGAACAAGAGGAAAACAGAAGAACATGAAATCTGTTTTAACTAATTGAAGATAATATGATGCTAACTTAGCGGTGTGCGTTTGACAATTACAGGTTGGACAATTGGCAAAAGATGTTCTTGTTAAAAGGTTACGATCAATTGCTGGAGATGAGATGCTTCTATCTATGATTCGTGAAATCCGTGGATGTCAATGAGGTTCTTCCAAGTTTTATTTGGAAGCATTTGGAATTTCAATAATTGTGCTTTACCATTTGATAGAACAAGTTGTCAGATGTTACCATACAAGTAGTTTTAGCATAAAAAGCTCAATATACCGTAGAAAGATTAGAAAAACATTTGTAATTTTTCCTTAAATACATCTTATGTGCAGAGATTTACTTAGAGTTCATCTTAGCTCTTGATTGCTAATCTACTCTGTGCCAATATATTCTTCTAATTCTACAAGAGGGGTGTTTGAATTGCCCATGAAATTGTAAATTTCAGACTAAATTATGGCTATGGATCTTCCCTAAAAAAGTATGAAATTTTTCTCCATTGGGTTTGCCCCTCTCCAACATTGTAATTTTCAAACTAAACTATCG

mRNA sequence

AAAAAAAAAAAAGACAAATAATAATAATAATAATAGAAGTCGCTAACTTAATTCCCAAGTAAGTCGATTTCAGCGGGGGAAGCTTCCGTGAATCGGCGCTTCACGTTCACAAGCATCCGTCAAATCGGCGATTTAGTCAATTACCCAAATACCCTCCTCTCCTTAAACTTTTTATTGCCGATAACCGACTTTGACTTAGCCGATTCGTAGGGCTATATGTGTAATTTGGTCTTATTTGGTGACTGGAAACGGATACATATGCCGGCACGGCCCTGTTTCTTTGCCTTGATTCTACTGGTTCTTTCGAAGCGAATTTCAGCGCCGGCGGCTTTCGATCACAGAGACTAGTAGAATCCATGGCCTCCAAGTTACTTATGCATAAGCAATTGAGGAAGAAGATGTTGGGATCAATTGAAGTTCGATCTCCTCTCAGAAACCCTACCAATTCTTCCTCCTCCTCTTCCCATCACTTCTCCGATTCTCCCCAGTCTCTGATTCAGAATTACTCCAATTTCAATACAAGTGGTTTACCTTCACGTTTTATGTTCTATTCGGACAGTTCGTGGGTTGATTTCCCTACCCAAGTGCTTGAAATCTTGCGAACGGGTTTCTTCGACCGGAAGCCGGCGATGGAATTGCAAATTGATGGATCGAAGTACGTATTTGATTTGTACAGGATGTTGCAGATTGATTTAGAAACTGGTAGACAGAGATCAGTTGCTTGGATCGATGAAAACGGTAAGTGTTTCTTCCCCAAAGTTTTTATTAGGGAAGAAAGTGTGAATACCTCCCGGACTTCTAGTAGTCCGAAAATTGAAATTGAGATTACGATTGATGGGAAATCCGGGAAGAGAAAGAGGGAGGCGATGGAAGAAAATGAAGTCAGTTCCAGTAATGAACATGTGCAAGTGAAGGCATCGAAAATACCGCGTGTAGTTGCGAATGATTCAGAAACTTCTGTATGGCCAAAGGCGAAGGCGCTGAATGAAGGGGATAGTGCGTACTCGTTAGTAAGCAATTTCTTTCTCCCTTCAATGAAAAAGGTTGATCCTAGTTTTTCAATCTCTGCAATTCATCAGTGTACCCGTACTGGGCCACTGGAAAAAGCTCGTCTCGATGTGTTTCTGAAGCAGAATGAGATCACAACGGCTGCTCGTGGTGTGTCTAATATGGTTTATGCATGGTATGGAGCTTCTTCAAAAACTTTGGCCGGAATTTTGGCTCATGGATTTGGGGAGCCAGTTCAGGTTCCTTCCTCTGATACCACCCATGGCATTGGAGTTTATTTATCTCCATTGGGATTGCCCTATTTAAGTTCTAAGCTATTGGAGGCCGATGGCAATGGAGTTAAACATATGGTTTTATGTCGAGTTATATTGGGTAAGATTGAAAAAGTTGAAGCAGGATCTCAACAATCTCATCCTTCGAGTACAGCCTTTGACACTGGTGTGGATGATCCAACATTTCCAACACGGTATATTGTATGGTGTAGCAATATGAACAGACACATTTTACCAGAATACATAGTGAGCTTTAAATCAACAAATTGTTTGCCGGGTAAGTTGAGAGAATCGACTGATACGAAGTATTCCCTAGTGAAATTACTTTCGAAGATGAAGAATTCCCTGCCTACTTCCAAAGTTCAAGAGGTTTCAACATTGTTTCAGAAATTTAAGGTTGGACAATTGGCAAAAGATGTTCTTGTTAAAAGGTTACGATCAATTGCTGGAGATGAGATGCTTCTATCTATGATTCGTGAAATCCGTGGATGTCAATGAGGTTCTTCCAAGTTTTATTTGGAAGCATTTGGAATTTCAATAATTGTGCTTTACCATTTGATAGAACAAGTTGTCAGATGTTACCATACAAGTAGTTTTAGCATAAAAAGCTCAATATACCGTAGAAAGATTAGAAAAACATTTGTAATTTTTCCTTAAATACATCTTATGTGCAGAGATTTACTTAGAGTTCATCTTAGCTCTTGATTGCTAATCTACTCTGTGCCAATATATTCTTCTAATTCTACAAGAGGGGTGTTTGAATTGCCCATGAAATTGTAAATTTCAGACTAAATTATGGCTATGGATCTTCCCTAAAAAAGTATGAAATTTTTCTCCATTGGGTTTGCCCCTCTCCAACATTGTAATTTTCAAACTAAACTATCG

Coding sequence (CDS)

ATGGCCTCCAAGTTACTTATGCATAAGCAATTGAGGAAGAAGATGTTGGGATCAATTGAAGTTCGATCTCCTCTCAGAAACCCTACCAATTCTTCCTCCTCCTCTTCCCATCACTTCTCCGATTCTCCCCAGTCTCTGATTCAGAATTACTCCAATTTCAATACAAGTGGTTTACCTTCACGTTTTATGTTCTATTCGGACAGTTCGTGGGTTGATTTCCCTACCCAAGTGCTTGAAATCTTGCGAACGGGTTTCTTCGACCGGAAGCCGGCGATGGAATTGCAAATTGATGGATCGAAGTACGTATTTGATTTGTACAGGATGTTGCAGATTGATTTAGAAACTGGTAGACAGAGATCAGTTGCTTGGATCGATGAAAACGGTAAGTGTTTCTTCCCCAAAGTTTTTATTAGGGAAGAAAGTGTGAATACCTCCCGGACTTCTAGTAGTCCGAAAATTGAAATTGAGATTACGATTGATGGGAAATCCGGGAAGAGAAAGAGGGAGGCGATGGAAGAAAATGAAGTCAGTTCCAGTAATGAACATGTGCAAGTGAAGGCATCGAAAATACCGCGTGTAGTTGCGAATGATTCAGAAACTTCTGTATGGCCAAAGGCGAAGGCGCTGAATGAAGGGGATAGTGCGTACTCGTTAGTAAGCAATTTCTTTCTCCCTTCAATGAAAAAGGTTGATCCTAGTTTTTCAATCTCTGCAATTCATCAGTGTACCCGTACTGGGCCACTGGAAAAAGCTCGTCTCGATGTGTTTCTGAAGCAGAATGAGATCACAACGGCTGCTCGTGGTGTGTCTAATATGGTTTATGCATGGTATGGAGCTTCTTCAAAAACTTTGGCCGGAATTTTGGCTCATGGATTTGGGGAGCCAGTTCAGGTTCCTTCCTCTGATACCACCCATGGCATTGGAGTTTATTTATCTCCATTGGGATTGCCCTATTTAAGTTCTAAGCTATTGGAGGCCGATGGCAATGGAGTTAAACATATGGTTTTATGTCGAGTTATATTGGGTAAGATTGAAAAAGTTGAAGCAGGATCTCAACAATCTCATCCTTCGAGTACAGCCTTTGACACTGGTGTGGATGATCCAACATTTCCAACACGGTATATTGTATGGTGTAGCAATATGAACAGACACATTTTACCAGAATACATAGTGAGCTTTAAATCAACAAATTGTTTGCCGGGTAAGTTGAGAGAATCGACTGATACGAAGTATTCCCTAGTGAAATTACTTTCGAAGATGAAGAATTCCCTGCCTACTTCCAAAGTTCAAGAGGTTTCAACATTGTTTCAGAAATTTAAGGTTGGACAATTGGCAAAAGATGTTCTTGTTAAAAGGTTACGATCAATTGCTGGAGATGAGATGCTTCTATCTATGATTCGTGAAATCCGTGGATGTCAATGA

Protein sequence

MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPSRFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSNEHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKMKNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ
Homology
BLAST of Lsi08G010570 vs. ExPASy Swiss-Prot
Match: Q8RY59 (Inactive poly [ADP-ribose] polymerase RCD1 OS=Arabidopsis thaliana OX=3702 GN=RCD1 PE=1 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.4e-44
Identity = 116/399 (29.07%), Postives = 197/399 (49.37%), Query Frame = 0

Query: 26  RNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPSRFMFYSDSSWVDFPTQVLEILRTGF 85
           R      +  S + + S ++L++ Y+ F  +G+  R M Y +  W D P  V+  ++   
Sbjct: 56  RRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNEL 115

Query: 86  FDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIREESVN-- 145
            ++  A+E ++ G  ++ D   M ++D+ETG +  +AWID  GKCFFP+++  +E  N  
Sbjct: 116 EEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYC 175

Query: 146 ------TSRTSSSPKIEIEITIDGKSGKRKREAMEE----------NEV----SSSNEHV 205
                   + ++   I++ + ID   G+  R  +EE          ++V     SSNEH 
Sbjct: 176 HHKCVEDPKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHY 235

Query: 206 QVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQCT 265
             +A++       ++  S W +  A+    +  +        ++KK+    + S  H   
Sbjct: 236 D-EATEDSCSRKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPV 295

Query: 266 R-----TGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQV 325
                 +  + +ARL +F KQ EIT   RG +N+ YAW  A  + L+ ++  G G     
Sbjct: 296 LDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAF 355

Query: 326 PSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSS 385
               + +G+G++L+    PY S++  + D NGV++MVLCRVI+G +E +     Q     
Sbjct: 356 -IRKSIYGVGIHLTAADCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGG 415

Query: 386 TAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTN 398
             +D GVDD   P  YIVW  NMN HI PE++V FK +N
Sbjct: 416 EEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSN 452

BLAST of Lsi08G010570 vs. ExPASy Swiss-Prot
Match: O82289 (Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3702 GN=SRO1 PE=1 SV=2)

HSP 1 Score: 181.0 bits (458), Expect = 3.1e-44
Identity = 121/418 (28.95%), Postives = 195/418 (46.65%), Query Frame = 0

Query: 44  QSLIQNYSNFNTSGLPSRFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVF 103
           +SL++ +S +  +G+P R MF+ +  W+D P  +L  +R     ++  +E    G  ++ 
Sbjct: 73  KSLVRYFSYYKKTGVPKRVMFHENGEWIDLPDHILCDIRNDLEAKRATIEFNWCGRHFLL 132

Query: 104 DLYRMLQIDLETGRQRSVAWIDENGKCFFPKVF----------IREESVNTSRTSSSPKI 163
           D   M ++DLETG +  +AWID  GKCFFP+ F          IR E           +I
Sbjct: 133 DFLHMYRLDLETGVKTQLAWIDIAGKCFFPETFDTLERDGCHHIRGED---PEQHDQREI 192

Query: 164 EIEITIDGKSGKRKREAMEENEVSSSNEHVQVKASKIPRVVANDSETSVWPKAKALNEGD 223
           ++ I ID  SG+  R  +      S +     +A +      ND E S    ++ L++  
Sbjct: 193 KLHIEIDVNSGELPRLNLNVVTDESGDNMDDFQAVQRSSNGPND-EASEDSCSRELDDAV 252

Query: 224 SAYSLVSNFFLPSMKKVDPSFSISAIHQCTRTG-----------------PLEKARLDVF 283
             +          +K  +      A+ Q    G                  + KARL +F
Sbjct: 253 EKWDKTETDRFSGVKPAEEELDKDAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLF 312

Query: 284 LKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPSSDTTHGIGVYLSPLGL 343
            KQ +IT   RG +N+ YAW  A  + L+ ++ HG G         + +G+GV+ +    
Sbjct: 313 QKQADITKKHRGDANIRYAWVPAKKEVLSAVMMHGLGVGGAF-IKKSMYGVGVHAA--NC 372

Query: 344 PYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTAFDTGVDDPTFPTRYIV 403
           PY S++  + D NGV+HMVLCRVI+G +E +   + Q       +D GVDD   P  Y++
Sbjct: 373 PYFSARYCDIDDNGVRHMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLI 432

Query: 404 WCSNMNRHILPEYIVSFK-STNCLPGKLRESTDTKYSLVKLLSKMKNSLPTSKVQEVS 434
           W  NMN HI PE++VSFK S     G +  +T +++    L  +     P+++   VS
Sbjct: 433 WNMNMNTHIYPEFVVSFKLSIPNAEGNILPTTQSRHESSGLTLEGPKGSPSNEPGRVS 483

BLAST of Lsi08G010570 vs. ExPASy Swiss-Prot
Match: O64592 (Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3702 GN=SRO3 PE=1 SV=2)

HSP 1 Score: 148.3 bits (373), Expect = 2.2e-34
Identity = 86/266 (32.33%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 209 LNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQ-CTRTGPLEKARLDVF-LKQNEITTAA 268
           L E    ++L+ N FL  M       +I  + +  T+     KA+  VF L    +    
Sbjct: 40  LREATFEHNLIKNCFLSGMGSFATETTIVTVRKILTQRLITTKAKFAVFKLFTEAMKRKN 99

Query: 269 RGVSNMVYAWYGASSKTLAGILAHGFG--EPVQVPSSDTTHGIGVYLSPLGLPYLSSKLL 328
            G +N+ Y WY  S + +  ++ +GF   E  +V +   +HG+G++L        ++ + 
Sbjct: 100 NGYANIRYGWYSGSKEEIDRVITYGFSNREIKKVENDVGSHGVGIHLVHHRYSLAAALVG 159

Query: 329 EADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTAFDTGVDDPTFPTRYIVWCSNMNRH 388
           E D  G+K+++LCRVILGK E++  GS+QS+PSS  FD+GVD+   P +Y++W  NMN +
Sbjct: 160 EGDEEGIKNILLCRVILGKPEQIVTGSKQSYPSSNQFDSGVDNLENPRKYVIWSCNMNSY 219

Query: 389 ILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKMKNSLPTSKVQEVSTLFQKFKVGQL 448
           ILP YIVSFKS + L G +  +     S   L+S +  SL  +++  + T +  F+  +L
Sbjct: 220 ILPTYIVSFKS-HLLRGLIGRARSPCVSFSVLMSILSKSLDAARMNLILTSYDDFRKRKL 279

Query: 449 AKDVLVKRLRSIAGDEMLLSMIREIR 471
            ++ LV+++R + GD +L  +++  R
Sbjct: 280 RREQLVRKIREVVGDNLLFKILKNQR 304

BLAST of Lsi08G010570 vs. ExPASy Swiss-Prot
Match: Q9ZUD9 (Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3702 GN=SRO2 PE=1 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 9.3e-33
Identity = 83/266 (31.20%), Postives = 141/266 (53.01%), Query Frame = 0

Query: 209 LNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQ-CTRTGPLEKARLDVF-LKQNEITTAA 268
           L EG+  + ++    L  M  V    +I  I +  +  G   +A+   F +  + +    
Sbjct: 48  LGEGNPEHDVIKTCLLSGMGVVSSDTTIVTISKNSSERGITTRAKFLAFRIFTDAVARKH 107

Query: 269 RGVSNMVYAWYGASSKTLAGILAHGFG--EPVQVPSSDTTHGIGVYLSPLGLPYLSSKLL 328
            G +N+ Y WY  S   +  I+++GF   +  +  +   +HGIG++L P     L++   
Sbjct: 108 GGDANVKYGWYAGSRDEIQRIISYGFSNRDVGKFENDGGSHGIGIHLVPSKCSLLAASAT 167

Query: 329 EADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTAFDTGVDDPTFPTRYIVWCSNMNRH 388
           E D  G+++++LCRVILGK E + +GS+QS+PSS  FD+GVDD   P  Y++W  NMN  
Sbjct: 168 EQDEEGLRYLLLCRVILGKPEIIISGSKQSYPSSAEFDSGVDDLHNPRNYVIWSCNMNSC 227

Query: 389 ILPEYIVSFKSTN---CLPGKLRESTDTKYSLVKLLSKMKNSLPTSKVQEVSTLFQKFKV 448
           ILP YIVSF+S        G     +    S   L+S +  S+  S++  +   +  F+ 
Sbjct: 228 ILPSYIVSFRSPRLRVSRGGFASRPSSPWVSFASLMSMLSTSMDPSRMNLIIRTYDDFRK 287

Query: 449 GQLAKDVLVKRLRSIAGDEMLLSMIR 468
            ++ +D LV+++R +AGD +L  +I+
Sbjct: 288 RKIRRDQLVRKMREVAGDNLLAEIIK 313

BLAST of Lsi08G010570 vs. ExPASy Swiss-Prot
Match: Q9FJJ3 (Probable inactive poly [ADP-ribose] polymerase SRO5 OS=Arabidopsis thaliana OX=3702 GN=SRO5 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 7.6e-27
Identity = 77/218 (35.32%), Postives = 124/218 (56.88%), Query Frame = 0

Query: 255 VFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPSSDTTHGIGVYLSPL 314
           VF +  ++     G + + Y W   S   L  I  +GF EP++   +D + G G+YLSP 
Sbjct: 91  VFQEAVQMKHGGDGGAKVKYGWCSVSKHELKTIFEYGFSEPLR---NDGSFGRGLYLSPD 150

Query: 315 GLPY--LSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTAFDTGVDDPTFPT 374
             P   L     E++ +G++ ++LCRV+LGK E V  GS +S PSS  FD+GVDD     
Sbjct: 151 NSPLDCLKDSASESE-DGMRFLLLCRVLLGKSEIVPQGSTRSCPSSPEFDSGVDDLVSTK 210

Query: 375 RYIVWCSNMNRHILPEYIVSFKSTNCL---PGKLRESTDTKYSLVKLLSKMKNSLPTSKV 434
           +YIVW ++MN H+LPE++V  K+   L   P +LR        L+K LSK    LP S++
Sbjct: 211 KYIVWSTHMNTHVLPEFLVCIKAPFNLTRSPKRLRSPWMAFPVLIKALSKF---LPPSQI 270

Query: 435 QEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIR 468
             +   ++  +  ++ +  L++R+RSI GD++L+ +I+
Sbjct: 271 LVIQKHYKDQQNRRITRSELIQRVRSITGDKLLVHIIK 301

BLAST of Lsi08G010570 vs. ExPASy TrEMBL
Match: A0A5A7VA62 (Poly [ADP-ribose] polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G003920 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.5e-224
Identity = 400/473 (84.57%), Postives = 428/473 (90.49%), Query Frame = 0

Query: 1   MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPS 60
           MASKLLMH+QLRKKMLGSIEVRSPL NPTNSSS      SDSP SL+ NYSNF  SGLPS
Sbjct: 1   MASKLLMHEQLRKKMLGSIEVRSPLGNPTNSSS------SDSPLSLLHNYSNFKKSGLPS 60

Query: 61  RFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRS 120
           RFMFY  SSWVDFPTQVLEILRT F DR PA+ELQIDGSKY+FDLYRMLQIDL+TGRQRS
Sbjct: 61  RFMFYFRSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQRS 120

Query: 121 VAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSN 180
           V+WID+ G CFFPKVFI EE+ N+S  S SPKIEIEIT+DGKSGKRKRE MEENEVSSSN
Sbjct: 121 VSWIDDKGNCFFPKVFIGEETANSSPISGSPKIEIEITVDGKSGKRKRETMEENEVSSSN 180

Query: 181 EHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIH 240
           EH++VKASKIPRVV NDSETSVWPK K LNEGDS YSLVSN  LPSMKKVD +FSISAIH
Sbjct: 181 EHMKVKASKIPRVVLNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFSISAIH 240

Query: 241 QCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPS 300
           +CTRTGPLEKARLDVFLKQNEIT AARGVSNMVYAWYGAS+KTLAGILAHGFGEPVQ+P+
Sbjct: 241 RCTRTGPLEKARLDVFLKQNEITAAARGVSNMVYAWYGASAKTLAGILAHGFGEPVQIPA 300

Query: 301 SDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTA 360
           SD THGIGVYLSP GLP+LSSKL EADGNGVKHM+LCRVILG +EK+EAGS+QSHPSST 
Sbjct: 301 SDATHGIGVYLSPWGLPHLSSKLSEADGNGVKHMILCRVILGNMEKIEAGSRQSHPSSTE 360

Query: 361 FDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKM 420
           FDTGVDDPT P RYIVWCSNMNRHILPEYIVSFKS++ LPGKLREST+TKY L KLLSKM
Sbjct: 361 FDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSSSHLPGKLRESTETKYPLAKLLSKM 420

Query: 421 KNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           +NSLP SKVQEV+TLFQKFKVGQLAKDVLVKRLRSIAGDEMLLS+ RE RGCQ
Sbjct: 421 RNSLPPSKVQEVATLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSIFRESRGCQ 467

BLAST of Lsi08G010570 vs. ExPASy TrEMBL
Match: A0A1S3BCD4 (Poly [ADP-ribose] polymerase OS=Cucumis melo OX=3656 GN=LOC103488157 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 3.5e-224
Identity = 400/473 (84.57%), Postives = 428/473 (90.49%), Query Frame = 0

Query: 1   MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPS 60
           MASKLLMH+QLRKKMLGSIEVRSPL NPTNSSS      SDSP SL+ NYSNF  SGLPS
Sbjct: 1   MASKLLMHEQLRKKMLGSIEVRSPLGNPTNSSS------SDSPLSLLHNYSNFKKSGLPS 60

Query: 61  RFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRS 120
           RFMFY  SSWVDFPTQVLEILRT F DR PA+ELQIDGSKY+FDLYRMLQIDL+TGRQRS
Sbjct: 61  RFMFYFRSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQRS 120

Query: 121 VAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSN 180
           V+WID+ G CFFPKVFI EE+ N+S  S SPKIEIEIT+DGKSGKRKRE MEENEVSSSN
Sbjct: 121 VSWIDDKGNCFFPKVFIGEETANSSPISGSPKIEIEITVDGKSGKRKRETMEENEVSSSN 180

Query: 181 EHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIH 240
           EH++VKASKIPRVV NDSETSVWPK K LNEGDS YSLVSN  LPSMKKVD +FSISAIH
Sbjct: 181 EHMKVKASKIPRVVMNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFSISAIH 240

Query: 241 QCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPS 300
           +CTRTGPLEKARLDVFLKQNEIT AARGVSNMVYAWYGAS+KTLAGILAHGFGEPVQ+P+
Sbjct: 241 RCTRTGPLEKARLDVFLKQNEITAAARGVSNMVYAWYGASAKTLAGILAHGFGEPVQIPA 300

Query: 301 SDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTA 360
           SD THGIGVYLSP GLP+LSSKL EADGNGVKHM+LCRVILG +EK+EAGS+QSHPSST 
Sbjct: 301 SDATHGIGVYLSPWGLPHLSSKLSEADGNGVKHMILCRVILGNMEKIEAGSRQSHPSSTE 360

Query: 361 FDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKM 420
           FDTGVDDPT P RYIVWCSNMNRHILPEYIVSFKS++ LPGKLREST+TKY L KLLSKM
Sbjct: 361 FDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSSSHLPGKLRESTETKYPLAKLLSKM 420

Query: 421 KNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           +NSLP SKVQEV+TLFQKFKVGQLAKDVLVKRLRSIAGDEMLLS+ RE RGCQ
Sbjct: 421 RNSLPPSKVQEVATLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSIFRESRGCQ 467

BLAST of Lsi08G010570 vs. ExPASy TrEMBL
Match: A0A0A0LLG4 (Poly [ADP-ribose] polymerase OS=Cucumis sativus OX=3659 GN=Csa_2G372800 PE=4 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.9e-223
Identity = 401/471 (85.14%), Postives = 428/471 (90.87%), Query Frame = 0

Query: 1   MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPS 60
           MASKLL+H+QLRKKMLGSIEVRSPL NPTNSSS      SDSP SL+ NYSNF  SGLPS
Sbjct: 1   MASKLLVHEQLRKKMLGSIEVRSPLGNPTNSSS------SDSPLSLLHNYSNFKRSGLPS 60

Query: 61  RFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRS 120
           RFMFY  SSWVDFPTQVLEILRT F DR PA+ELQIDGSKY+FDLYRMLQIDL+TGRQRS
Sbjct: 61  RFMFYFHSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQRS 120

Query: 121 VAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSN 180
           VAWID+ G+CFFPKVFI EE+ N+S  S SPKIEI ITIDGKSGKRKRE MEENEVSSSN
Sbjct: 121 VAWIDDKGECFFPKVFIGEETTNSSPISCSPKIEISITIDGKSGKRKRETMEENEVSSSN 180

Query: 181 EHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIH 240
           EH++VKASKIPRVV NDSETSVWPK K LNEGDS YSLVSN  LPSMKKVD +FSISAIH
Sbjct: 181 EHMKVKASKIPRVVMNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFSISAIH 240

Query: 241 QCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPS 300
           +CTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGAS+KTLAGILAHGFGEPVQ+P+
Sbjct: 241 RCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASAKTLAGILAHGFGEPVQIPA 300

Query: 301 SDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTA 360
           SDTTHGIGVYLSPLGLP+LSSKL EADGNGVKHM+LCRVILG +EKV AGS+QSHPSST 
Sbjct: 301 SDTTHGIGVYLSPLGLPHLSSKLSEADGNGVKHMILCRVILGNMEKVGAGSRQSHPSSTE 360

Query: 361 FDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKM 420
           FDTGVDDPT P RYIVWCSNMNRHILPEYIVSFKST+ LPG LREST+TKY LVKLLSKM
Sbjct: 361 FDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSTSHLPGNLRESTETKYPLVKLLSKM 420

Query: 421 KNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRG 472
           +NSLP SKVQEV+TLFQKFKVGQLAKDVLVKRLRSIAGD+MLLS+ RE RG
Sbjct: 421 RNSLPPSKVQEVATLFQKFKVGQLAKDVLVKRLRSIAGDQMLLSIFRESRG 465

BLAST of Lsi08G010570 vs. ExPASy TrEMBL
Match: A0A6J1GJV8 (Poly [ADP-ribose] polymerase OS=Cucurbita moschata OX=3662 GN=LOC111454523 PE=4 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 3.2e-209
Identity = 382/480 (79.58%), Postives = 418/480 (87.08%), Query Frame = 0

Query: 2   ASKLLMHKQLRKKMLGSIEVRSP----LRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSG 61
           +SKLLMHKQ RKKM+ S+ VR+P     RNP +  SSS HH SDS QSLIQNYSNF  SG
Sbjct: 43  SSKLLMHKQFRKKMVESVAVRAPPKPCSRNPID--SSSCHHLSDSSQSLIQNYSNFKASG 102

Query: 62  LPSRFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGR 121
           LPSRFMFY D  WVDF  +V+ ILR GF DR PA+EL+IDGS Y+FD+YRMLQIDLETGR
Sbjct: 103 LPSRFMFYQDRLWVDFDNRVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGR 162

Query: 122 QRSVAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAM----EE 181
           QRSVAWIDENGKCFFPKVFI +ES   +R+ S+PKIEIEI ID KSGKRKREAM    EE
Sbjct: 163 QRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKIDRKSGKRKREAMEMEEEE 222

Query: 182 NEVSSSNEHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPS 241
           NEV+SSNEHV+VK  KIPRVVANDSE  VWPKAKAL+EGDSAY+LVSNFFLPSMKKVDPS
Sbjct: 223 NEVNSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPS 282

Query: 242 FSISAIHQCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFG 301
            +I++IHQCTRTGPLEKARLDVF KQNEITTAARGVSNMVYAWYGAS+ +LAGILAHGFG
Sbjct: 283 SAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFG 342

Query: 302 EPVQVPSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQ 361
           EP+QVP++D THGIGVYLSPLGLP+L S L E D +G+KHMVLCRVILG +EKVEAGSQQ
Sbjct: 343 EPLQVPAAD-THGIGVYLSPLGLPHLCSTLSEVDEDGIKHMVLCRVILGNVEKVEAGSQQ 402

Query: 362 SHPSSTAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSL 421
           SHPSST FDTGVDDPT P RYIVWCSNMNRHILPEYIVSF+S   LPGKL ESTDTKYSL
Sbjct: 403 SHPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSL 462

Query: 422 VKLLSKMKNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           VKLLSKMKNSLPTSKVQEV+TLFQ FK G+L KDVLVKRLRSIAGD+MLLS+IREIR CQ
Sbjct: 463 VKLLSKMKNSLPTSKVQEVATLFQTFKAGKLGKDVLVKRLRSIAGDDMLLSLIREIRECQ 519

BLAST of Lsi08G010570 vs. ExPASy TrEMBL
Match: A0A6J1KNM2 (Poly [ADP-ribose] polymerase OS=Cucurbita maxima OX=3661 GN=LOC111496198 PE=4 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 1.6e-208
Identity = 381/480 (79.38%), Postives = 418/480 (87.08%), Query Frame = 0

Query: 2   ASKLLMHKQLRKKMLGSIEVRSP----LRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSG 61
           +SKLLMHKQ RKKM+ S+ V +P     RNP N  SSS +H SDS QSLIQNYSNF  SG
Sbjct: 3   SSKLLMHKQFRKKMVESVAVPAPPRPCSRNPIN--SSSCNHLSDSSQSLIQNYSNFKASG 62

Query: 62  LPSRFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGR 121
           LPSRFMFY D  WVDF TQV+ ILR GF DR PA+EL+IDGS Y+FD+YRMLQIDLETGR
Sbjct: 63  LPSRFMFYQDRLWVDFDTQVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGR 122

Query: 122 QRSVAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAM----EE 181
           QRSVAWIDENGKCFFPKVFI +ES   +R+ S+PKIEIEI  DGKSGKRKREAM    EE
Sbjct: 123 QRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKFDGKSGKRKREAMEMEEEE 182

Query: 182 NEVSSSNEHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPS 241
           N V+SSNEHV+VK  K+PRVVANDSE  VWPKAKAL+EGDSAY+LVSNFFLPS+KKVDPS
Sbjct: 183 NYVNSSNEHVEVKPLKMPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSIKKVDPS 242

Query: 242 FSISAIHQCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFG 301
            +I++IHQCTRTGPLEKARLDVF KQNEITTAARGVSNMVYAWYGAS+ +LAGILAHGFG
Sbjct: 243 SAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFG 302

Query: 302 EPVQVPSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQ 361
           EP+QVP++D THGIGVYLSPLGLP+L S L EAD +G+KHMVLCRVILG +EKVEAGSQQ
Sbjct: 303 EPLQVPAAD-THGIGVYLSPLGLPHLCSTLSEADEDGIKHMVLCRVILGNVEKVEAGSQQ 362

Query: 362 SHPSSTAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSL 421
           SHPSST FDTGVDDPT P RYIVWCSNMNRHILPEYIVSF+S   LPGKL ESTDTKYSL
Sbjct: 363 SHPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSL 422

Query: 422 VKLLSKMKNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           VKLLSKMKNSLPTSKVQEV+TLFQ FK G+L KDVLVKRLRSIAGD+MLLS+IREIR CQ
Sbjct: 423 VKLLSKMKNSLPTSKVQEVATLFQTFKAGKLGKDVLVKRLRSIAGDDMLLSLIREIRECQ 479

BLAST of Lsi08G010570 vs. NCBI nr
Match: XP_038885304.1 (probable inactive poly [ADP-ribose] polymerase SRO3 [Benincasa hispida])

HSP 1 Score: 853.6 bits (2204), Expect = 8.1e-244
Identity = 437/474 (92.19%), Postives = 452/474 (95.36%), Query Frame = 0

Query: 1   MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPS 60
           MASKLLMH+QLRKKMLGS EVRSPLRNPT+SSSSS +HFSDSPQSLIQNYSNF TSGLPS
Sbjct: 1   MASKLLMHEQLRKKMLGSTEVRSPLRNPTDSSSSSCNHFSDSPQSLIQNYSNFKTSGLPS 60

Query: 61  RFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRS 120
           RFMFY DSSWVDFPTQVLEILRTGF DRKPAMEL+IDGSKY+FDLYRMLQIDL+TGRQRS
Sbjct: 61  RFMFYLDSSWVDFPTQVLEILRTGFSDRKPAMELKIDGSKYLFDLYRMLQIDLDTGRQRS 120

Query: 121 VAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSN 180
           VAWIDENGKCFFPKVFI EES NTSRTS S KIEIEITIDGK GKRKRE +EENEV+SSN
Sbjct: 121 VAWIDENGKCFFPKVFIGEESANTSRTSCSRKIEIEITIDGKYGKRKREEIEENEVNSSN 180

Query: 181 EHVQV-KASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAI 240
           EHV V KASKIPRVVANDSETSVWPK KALNEGDSAYSLVSNF LPSMKKVDPSFSISAI
Sbjct: 181 EHVDVKKASKIPRVVANDSETSVWPKTKALNEGDSAYSLVSNFLLPSMKKVDPSFSISAI 240

Query: 241 HQCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVP 300
           HQCT TGPLEKARLDVFLKQNEITTA RGVSNMVYAWYGAS++TLAGILAHGFGEPVQVP
Sbjct: 241 HQCTHTGPLEKARLDVFLKQNEITTAVRGVSNMVYAWYGASARTLAGILAHGFGEPVQVP 300

Query: 301 SSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSST 360
           SSDTTHGIGVYLSPLGLP+LSSKL EADGNGVKH+VLCRVILGKIEKVEAGSQQ HPSST
Sbjct: 301 SSDTTHGIGVYLSPLGLPHLSSKLSEADGNGVKHVVLCRVILGKIEKVEAGSQQCHPSST 360

Query: 361 AFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSK 420
            FDTGVDDPTFP RYIVWCSNMNRHILPEYIVS+KSTN LPGKLREST+TKYSLVKLLSK
Sbjct: 361 EFDTGVDDPTFPKRYIVWCSNMNRHILPEYIVSYKSTNYLPGKLRESTETKYSLVKLLSK 420

Query: 421 MKNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           MKNSLPTSKVQEV+TLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ
Sbjct: 421 MKNSLPTSKVQEVATLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474

BLAST of Lsi08G010570 vs. NCBI nr
Match: KAA0065063.1 (putative inactive poly [Cucumis melo var. makuwa])

HSP 1 Score: 787.3 bits (2032), Expect = 7.2e-224
Identity = 400/473 (84.57%), Postives = 428/473 (90.49%), Query Frame = 0

Query: 1   MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPS 60
           MASKLLMH+QLRKKMLGSIEVRSPL NPTNSSS      SDSP SL+ NYSNF  SGLPS
Sbjct: 1   MASKLLMHEQLRKKMLGSIEVRSPLGNPTNSSS------SDSPLSLLHNYSNFKKSGLPS 60

Query: 61  RFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRS 120
           RFMFY  SSWVDFPTQVLEILRT F DR PA+ELQIDGSKY+FDLYRMLQIDL+TGRQRS
Sbjct: 61  RFMFYFRSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQRS 120

Query: 121 VAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSN 180
           V+WID+ G CFFPKVFI EE+ N+S  S SPKIEIEIT+DGKSGKRKRE MEENEVSSSN
Sbjct: 121 VSWIDDKGNCFFPKVFIGEETANSSPISGSPKIEIEITVDGKSGKRKRETMEENEVSSSN 180

Query: 181 EHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIH 240
           EH++VKASKIPRVV NDSETSVWPK K LNEGDS YSLVSN  LPSMKKVD +FSISAIH
Sbjct: 181 EHMKVKASKIPRVVLNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFSISAIH 240

Query: 241 QCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPS 300
           +CTRTGPLEKARLDVFLKQNEIT AARGVSNMVYAWYGAS+KTLAGILAHGFGEPVQ+P+
Sbjct: 241 RCTRTGPLEKARLDVFLKQNEITAAARGVSNMVYAWYGASAKTLAGILAHGFGEPVQIPA 300

Query: 301 SDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTA 360
           SD THGIGVYLSP GLP+LSSKL EADGNGVKHM+LCRVILG +EK+EAGS+QSHPSST 
Sbjct: 301 SDATHGIGVYLSPWGLPHLSSKLSEADGNGVKHMILCRVILGNMEKIEAGSRQSHPSSTE 360

Query: 361 FDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKM 420
           FDTGVDDPT P RYIVWCSNMNRHILPEYIVSFKS++ LPGKLREST+TKY L KLLSKM
Sbjct: 361 FDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSSSHLPGKLRESTETKYPLAKLLSKM 420

Query: 421 KNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           +NSLP SKVQEV+TLFQKFKVGQLAKDVLVKRLRSIAGDEMLLS+ RE RGCQ
Sbjct: 421 RNSLPPSKVQEVATLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSIFRESRGCQ 467

BLAST of Lsi08G010570 vs. NCBI nr
Match: XP_008444977.1 (PREDICTED: probable inactive poly [ADP-ribose] polymerase SRO3 [Cucumis melo])

HSP 1 Score: 787.3 bits (2032), Expect = 7.2e-224
Identity = 400/473 (84.57%), Postives = 428/473 (90.49%), Query Frame = 0

Query: 1   MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPS 60
           MASKLLMH+QLRKKMLGSIEVRSPL NPTNSSS      SDSP SL+ NYSNF  SGLPS
Sbjct: 1   MASKLLMHEQLRKKMLGSIEVRSPLGNPTNSSS------SDSPLSLLHNYSNFKKSGLPS 60

Query: 61  RFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRS 120
           RFMFY  SSWVDFPTQVLEILRT F DR PA+ELQIDGSKY+FDLYRMLQIDL+TGRQRS
Sbjct: 61  RFMFYFRSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQRS 120

Query: 121 VAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSN 180
           V+WID+ G CFFPKVFI EE+ N+S  S SPKIEIEIT+DGKSGKRKRE MEENEVSSSN
Sbjct: 121 VSWIDDKGNCFFPKVFIGEETANSSPISGSPKIEIEITVDGKSGKRKRETMEENEVSSSN 180

Query: 181 EHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIH 240
           EH++VKASKIPRVV NDSETSVWPK K LNEGDS YSLVSN  LPSMKKVD +FSISAIH
Sbjct: 181 EHMKVKASKIPRVVMNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFSISAIH 240

Query: 241 QCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPS 300
           +CTRTGPLEKARLDVFLKQNEIT AARGVSNMVYAWYGAS+KTLAGILAHGFGEPVQ+P+
Sbjct: 241 RCTRTGPLEKARLDVFLKQNEITAAARGVSNMVYAWYGASAKTLAGILAHGFGEPVQIPA 300

Query: 301 SDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTA 360
           SD THGIGVYLSP GLP+LSSKL EADGNGVKHM+LCRVILG +EK+EAGS+QSHPSST 
Sbjct: 301 SDATHGIGVYLSPWGLPHLSSKLSEADGNGVKHMILCRVILGNMEKIEAGSRQSHPSSTE 360

Query: 361 FDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKM 420
           FDTGVDDPT P RYIVWCSNMNRHILPEYIVSFKS++ LPGKLREST+TKY L KLLSKM
Sbjct: 361 FDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSSSHLPGKLRESTETKYPLAKLLSKM 420

Query: 421 KNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           +NSLP SKVQEV+TLFQKFKVGQLAKDVLVKRLRSIAGDEMLLS+ RE RGCQ
Sbjct: 421 RNSLPPSKVQEVATLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSIFRESRGCQ 467

BLAST of Lsi08G010570 vs. NCBI nr
Match: XP_004148400.1 (probable inactive poly [ADP-ribose] polymerase SRO3 [Cucumis sativus] >KGN62785.1 hypothetical protein Csa_022132 [Cucumis sativus])

HSP 1 Score: 784.3 bits (2024), Expect = 6.1e-223
Identity = 401/471 (85.14%), Postives = 428/471 (90.87%), Query Frame = 0

Query: 1   MASKLLMHKQLRKKMLGSIEVRSPLRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPS 60
           MASKLL+H+QLRKKMLGSIEVRSPL NPTNSSS      SDSP SL+ NYSNF  SGLPS
Sbjct: 1   MASKLLVHEQLRKKMLGSIEVRSPLGNPTNSSS------SDSPLSLLHNYSNFKRSGLPS 60

Query: 61  RFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRS 120
           RFMFY  SSWVDFPTQVLEILRT F DR PA+ELQIDGSKY+FDLYRMLQIDL+TGRQRS
Sbjct: 61  RFMFYFHSSWVDFPTQVLEILRTAFSDRNPAVELQIDGSKYLFDLYRMLQIDLDTGRQRS 120

Query: 121 VAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAMEENEVSSSN 180
           VAWID+ G+CFFPKVFI EE+ N+S  S SPKIEI ITIDGKSGKRKRE MEENEVSSSN
Sbjct: 121 VAWIDDKGECFFPKVFIGEETTNSSPISCSPKIEISITIDGKSGKRKRETMEENEVSSSN 180

Query: 181 EHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIH 240
           EH++VKASKIPRVV NDSETSVWPK K LNEGDS YSLVSN  LPSMKKVD +FSISAIH
Sbjct: 181 EHMKVKASKIPRVVMNDSETSVWPKTKVLNEGDSGYSLVSNSLLPSMKKVDSTFSISAIH 240

Query: 241 QCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPS 300
           +CTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGAS+KTLAGILAHGFGEPVQ+P+
Sbjct: 241 RCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASAKTLAGILAHGFGEPVQIPA 300

Query: 301 SDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTA 360
           SDTTHGIGVYLSPLGLP+LSSKL EADGNGVKHM+LCRVILG +EKV AGS+QSHPSST 
Sbjct: 301 SDTTHGIGVYLSPLGLPHLSSKLSEADGNGVKHMILCRVILGNMEKVGAGSRQSHPSSTE 360

Query: 361 FDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKM 420
           FDTGVDDPT P RYIVWCSNMNRHILPEYIVSFKST+ LPG LREST+TKY LVKLLSKM
Sbjct: 361 FDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFKSTSHLPGNLRESTETKYPLVKLLSKM 420

Query: 421 KNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRG 472
           +NSLP SKVQEV+TLFQKFKVGQLAKDVLVKRLRSIAGD+MLLS+ RE RG
Sbjct: 421 RNSLPPSKVQEVATLFQKFKVGQLAKDVLVKRLRSIAGDQMLLSIFRESRG 465

BLAST of Lsi08G010570 vs. NCBI nr
Match: XP_023537456.1 (probable inactive poly [ADP-ribose] polymerase SRO3 [Cucurbita pepo subsp. pepo] >XP_023537458.1 probable inactive poly [ADP-ribose] polymerase SRO3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 742.7 bits (1916), Expect = 2.0e-210
Identity = 385/480 (80.21%), Postives = 421/480 (87.71%), Query Frame = 0

Query: 2   ASKLLMHKQLRKKMLGSIEVRSP----LRNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSG 61
           +SKLLMHKQ RKKM+ S+ VR+P     RNP +  SSS +H SDS QSLIQNYSNF  SG
Sbjct: 3   SSKLLMHKQFRKKMVESVAVRAPPRPCSRNPID--SSSCNHLSDSSQSLIQNYSNFKASG 62

Query: 62  LPSRFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVFDLYRMLQIDLETGR 121
           LPSRFMFY D  WVDF T+V+ ILR GF DR PA+EL+IDGS Y+FD+YRMLQIDLETGR
Sbjct: 63  LPSRFMFYQDRLWVDFDTRVIGILRAGFSDRNPAIELEIDGSTYLFDMYRMLQIDLETGR 122

Query: 122 QRSVAWIDENGKCFFPKVFIREESVNTSRTSSSPKIEIEITIDGKSGKRKREAM----EE 181
           QRSVAWIDENGKCFFPKVFI +ES   +R+ S+PKIEIEI IDGKSGKRKREAM    EE
Sbjct: 123 QRSVAWIDENGKCFFPKVFIGDESEGNTRSLSNPKIEIEIKIDGKSGKRKREAMEMKEEE 182

Query: 182 NEVSSSNEHVQVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPS 241
           NEVSSSNEHV+VK  KIPRVVANDSE  VWPKAKAL+EGDSAY+LVSNFFLPSMKKVDPS
Sbjct: 183 NEVSSSNEHVEVKPLKIPRVVANDSEAHVWPKAKALSEGDSAYTLVSNFFLPSMKKVDPS 242

Query: 242 FSISAIHQCTRTGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFG 301
            +I++IHQCTRTGPLEKARLDVF KQNEITTAARGVSNMVYAWYGAS+ +LAGILAHGFG
Sbjct: 243 SAITSIHQCTRTGPLEKARLDVFQKQNEITTAARGVSNMVYAWYGASATSLAGILAHGFG 302

Query: 302 EPVQVPSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQ 361
           EP+QVP++D THGIGVYLSPLGLP+L S L EAD +G+KHMVLCRVILG +EKVEAGSQQ
Sbjct: 303 EPLQVPAAD-THGIGVYLSPLGLPHLCSTLSEADEDGIKHMVLCRVILGNVEKVEAGSQQ 362

Query: 362 SHPSSTAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTNCLPGKLRESTDTKYSL 421
           SHPSST FDTGVDDPT P RYIVWCSNMNRHILPEYIVSF+S   LPGKL ESTDTKYSL
Sbjct: 363 SHPSSTEFDTGVDDPTCPKRYIVWCSNMNRHILPEYIVSFRSNYRLPGKLGESTDTKYSL 422

Query: 422 VKLLSKMKNSLPTSKVQEVSTLFQKFKVGQLAKDVLVKRLRSIAGDEMLLSMIREIRGCQ 474
           VKLLSKMKNSLPTSKVQEV+TLFQ FK G+L KDVLVKRLRSIAGD+MLLS+IREIR CQ
Sbjct: 423 VKLLSKMKNSLPTSKVQEVATLFQTFKAGKLGKDVLVKRLRSIAGDDMLLSLIREIRECQ 479

BLAST of Lsi08G010570 vs. TAIR 10
Match: AT1G32230.1 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 181.4 bits (459), Expect = 1.7e-45
Identity = 116/399 (29.07%), Postives = 197/399 (49.37%), Query Frame = 0

Query: 26  RNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPSRFMFYSDSSWVDFPTQVLEILRTGF 85
           R      +  S + + S ++L++ Y+ F  +G+  R M Y +  W D P  V+  ++   
Sbjct: 56  RRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNEL 115

Query: 86  FDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIREESVN-- 145
            ++  A+E ++ G  ++ D   M ++D+ETG +  +AWID  GKCFFP+++  +E  N  
Sbjct: 116 EEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYC 175

Query: 146 ------TSRTSSSPKIEIEITIDGKSGKRKREAMEE----------NEV----SSSNEHV 205
                   + ++   I++ + ID   G+  R  +EE          ++V     SSNEH 
Sbjct: 176 HHKCVEDPKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHY 235

Query: 206 QVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQCT 265
             +A++       ++  S W +  A+    +  +        ++KK+    + S  H   
Sbjct: 236 D-EATEDSCSRKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPV 295

Query: 266 R-----TGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQV 325
                 +  + +ARL +F KQ EIT   RG +N+ YAW  A  + L+ ++  G G     
Sbjct: 296 LDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAF 355

Query: 326 PSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSS 385
               + +G+G++L+    PY S++  + D NGV++MVLCRVI+G +E +     Q     
Sbjct: 356 -IRKSIYGVGIHLTAADCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGG 415

Query: 386 TAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTN 398
             +D GVDD   P  YIVW  NMN HI PE++V FK +N
Sbjct: 416 EEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSN 452

BLAST of Lsi08G010570 vs. TAIR 10
Match: AT1G32230.2 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 181.4 bits (459), Expect = 1.7e-45
Identity = 116/399 (29.07%), Postives = 197/399 (49.37%), Query Frame = 0

Query: 26  RNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPSRFMFYSDSSWVDFPTQVLEILRTGF 85
           R      +  S + + S ++L++ Y+ F  +G+  R M Y +  W D P  V+  ++   
Sbjct: 56  RRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNEL 115

Query: 86  FDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIREESVN-- 145
            ++  A+E ++ G  ++ D   M ++D+ETG +  +AWID  GKCFFP+++  +E  N  
Sbjct: 116 EEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYC 175

Query: 146 ------TSRTSSSPKIEIEITIDGKSGKRKREAMEE----------NEV----SSSNEHV 205
                   + ++   I++ + ID   G+  R  +EE          ++V     SSNEH 
Sbjct: 176 HHKCVEDPKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHY 235

Query: 206 QVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQCT 265
             +A++       ++  S W +  A+    +  +        ++KK+    + S  H   
Sbjct: 236 D-EATEDSCSRKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPV 295

Query: 266 R-----TGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQV 325
                 +  + +ARL +F KQ EIT   RG +N+ YAW  A  + L+ ++  G G     
Sbjct: 296 LDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAF 355

Query: 326 PSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSS 385
               + +G+G++L+    PY S++  + D NGV++MVLCRVI+G +E +     Q     
Sbjct: 356 -IRKSIYGVGIHLTAADCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGG 415

Query: 386 TAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTN 398
             +D GVDD   P  YIVW  NMN HI PE++V FK +N
Sbjct: 416 EEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSN 452

BLAST of Lsi08G010570 vs. TAIR 10
Match: AT1G32230.3 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 181.4 bits (459), Expect = 1.7e-45
Identity = 116/399 (29.07%), Postives = 197/399 (49.37%), Query Frame = 0

Query: 26  RNPTNSSSSSSHHFSDSPQSLIQNYSNFNTSGLPSRFMFYSDSSWVDFPTQVLEILRTGF 85
           R      +  S + + S ++L++ Y+ F  +G+  R M Y +  W D P  V+  ++   
Sbjct: 56  RRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQNEL 115

Query: 86  FDRKPAMELQIDGSKYVFDLYRMLQIDLETGRQRSVAWIDENGKCFFPKVFIREESVN-- 145
            ++  A+E ++ G  ++ D   M ++D+ETG +  +AWID  GKCFFP+++  +E  N  
Sbjct: 116 EEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERTNYC 175

Query: 146 ------TSRTSSSPKIEIEITIDGKSGKRKREAMEE----------NEV----SSSNEHV 205
                   + ++   I++ + ID   G+  R  +EE          ++V     SSNEH 
Sbjct: 176 HHKCVEDPKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESGDNMMDDVPLAQRSSNEHY 235

Query: 206 QVKASKIPRVVANDSETSVWPKAKALNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQCT 265
             +A++       ++  S W +  A+    +  +        ++KK+    + S  H   
Sbjct: 236 D-EATEDSCSRKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFAVGTASLGHVPV 295

Query: 266 R-----TGPLEKARLDVFLKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQV 325
                 +  + +ARL +F KQ EIT   RG +N+ YAW  A  + L+ ++  G G     
Sbjct: 296 LDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAVMMQGLGVGGAF 355

Query: 326 PSSDTTHGIGVYLSPLGLPYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSS 385
               + +G+G++L+    PY S++  + D NGV++MVLCRVI+G +E +     Q     
Sbjct: 356 -IRKSIYGVGIHLTAADCPYFSARYCDVDENGVRYMVLCRVIMGNMELLRGDKAQFFSGG 415

Query: 386 TAFDTGVDDPTFPTRYIVWCSNMNRHILPEYIVSFKSTN 398
             +D GVDD   P  YIVW  NMN HI PE++V FK +N
Sbjct: 416 EEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSN 452

BLAST of Lsi08G010570 vs. TAIR 10
Match: AT2G35510.1 (similar to RCD one 1 )

HSP 1 Score: 181.0 bits (458), Expect = 2.2e-45
Identity = 121/418 (28.95%), Postives = 195/418 (46.65%), Query Frame = 0

Query: 44  QSLIQNYSNFNTSGLPSRFMFYSDSSWVDFPTQVLEILRTGFFDRKPAMELQIDGSKYVF 103
           +SL++ +S +  +G+P R MF+ +  W+D P  +L  +R     ++  +E    G  ++ 
Sbjct: 73  KSLVRYFSYYKKTGVPKRVMFHENGEWIDLPDHILCDIRNDLEAKRATIEFNWCGRHFLL 132

Query: 104 DLYRMLQIDLETGRQRSVAWIDENGKCFFPKVF----------IREESVNTSRTSSSPKI 163
           D   M ++DLETG +  +AWID  GKCFFP+ F          IR E           +I
Sbjct: 133 DFLHMYRLDLETGVKTQLAWIDIAGKCFFPETFDTLERDGCHHIRGED---PEQHDQREI 192

Query: 164 EIEITIDGKSGKRKREAMEENEVSSSNEHVQVKASKIPRVVANDSETSVWPKAKALNEGD 223
           ++ I ID  SG+  R  +      S +     +A +      ND E S    ++ L++  
Sbjct: 193 KLHIEIDVNSGELPRLNLNVVTDESGDNMDDFQAVQRSSNGPND-EASEDSCSRELDDAV 252

Query: 224 SAYSLVSNFFLPSMKKVDPSFSISAIHQCTRTG-----------------PLEKARLDVF 283
             +          +K  +      A+ Q    G                  + KARL +F
Sbjct: 253 EKWDKTETDRFSGVKPAEEELDKDAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLF 312

Query: 284 LKQNEITTAARGVSNMVYAWYGASSKTLAGILAHGFGEPVQVPSSDTTHGIGVYLSPLGL 343
            KQ +IT   RG +N+ YAW  A  + L+ ++ HG G         + +G+GV+ +    
Sbjct: 313 QKQADITKKHRGDANIRYAWVPAKKEVLSAVMMHGLGVGGAF-IKKSMYGVGVHAA--NC 372

Query: 344 PYLSSKLLEADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTAFDTGVDDPTFPTRYIV 403
           PY S++  + D NGV+HMVLCRVI+G +E +   + Q       +D GVDD   P  Y++
Sbjct: 373 PYFSARYCDIDDNGVRHMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLI 432

Query: 404 WCSNMNRHILPEYIVSFK-STNCLPGKLRESTDTKYSLVKLLSKMKNSLPTSKVQEVS 434
           W  NMN HI PE++VSFK S     G +  +T +++    L  +     P+++   VS
Sbjct: 433 WNMNMNTHIYPEFVVSFKLSIPNAEGNILPTTQSRHESSGLTLEGPKGSPSNEPGRVS 483

BLAST of Lsi08G010570 vs. TAIR 10
Match: AT1G70440.1 (similar to RCD one 3 )

HSP 1 Score: 148.3 bits (373), Expect = 1.6e-35
Identity = 86/266 (32.33%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 209 LNEGDSAYSLVSNFFLPSMKKVDPSFSISAIHQ-CTRTGPLEKARLDVF-LKQNEITTAA 268
           L E    ++L+ N FL  M       +I  + +  T+     KA+  VF L    +    
Sbjct: 40  LREATFEHNLIKNCFLSGMGSFATETTIVTVRKILTQRLITTKAKFAVFKLFTEAMKRKN 99

Query: 269 RGVSNMVYAWYGASSKTLAGILAHGFG--EPVQVPSSDTTHGIGVYLSPLGLPYLSSKLL 328
            G +N+ Y WY  S + +  ++ +GF   E  +V +   +HG+G++L        ++ + 
Sbjct: 100 NGYANIRYGWYSGSKEEIDRVITYGFSNREIKKVENDVGSHGVGIHLVHHRYSLAAALVG 159

Query: 329 EADGNGVKHMVLCRVILGKIEKVEAGSQQSHPSSTAFDTGVDDPTFPTRYIVWCSNMNRH 388
           E D  G+K+++LCRVILGK E++  GS+QS+PSS  FD+GVD+   P +Y++W  NMN +
Sbjct: 160 EGDEEGIKNILLCRVILGKPEQIVTGSKQSYPSSNQFDSGVDNLENPRKYVIWSCNMNSY 219

Query: 389 ILPEYIVSFKSTNCLPGKLRESTDTKYSLVKLLSKMKNSLPTSKVQEVSTLFQKFKVGQL 448
           ILP YIVSFKS + L G +  +     S   L+S +  SL  +++  + T +  F+  +L
Sbjct: 220 ILPTYIVSFKS-HLLRGLIGRARSPCVSFSVLMSILSKSLDAARMNLILTSYDDFRKRKL 279

Query: 449 AKDVLVKRLRSIAGDEMLLSMIREIR 471
            ++ LV+++R + GD +L  +++  R
Sbjct: 280 RREQLVRKIREVVGDNLLFKILKNQR 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RY592.4e-4429.07Inactive poly [ADP-ribose] polymerase RCD1 OS=Arabidopsis thaliana OX=3702 GN=RC... [more]
O822893.1e-4428.95Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3... [more]
O645922.2e-3432.33Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3... [more]
Q9ZUD99.3e-3331.20Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3... [more]
Q9FJJ37.6e-2735.32Probable inactive poly [ADP-ribose] polymerase SRO5 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A5A7VA623.5e-22484.57Poly [ADP-ribose] polymerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BCD43.5e-22484.57Poly [ADP-ribose] polymerase OS=Cucumis melo OX=3656 GN=LOC103488157 PE=4 SV=1[more]
A0A0A0LLG42.9e-22385.14Poly [ADP-ribose] polymerase OS=Cucumis sativus OX=3659 GN=Csa_2G372800 PE=4 SV=... [more]
A0A6J1GJV83.2e-20979.58Poly [ADP-ribose] polymerase OS=Cucurbita moschata OX=3662 GN=LOC111454523 PE=4 ... [more]
A0A6J1KNM21.6e-20879.38Poly [ADP-ribose] polymerase OS=Cucurbita maxima OX=3661 GN=LOC111496198 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_038885304.18.1e-24492.19probable inactive poly [ADP-ribose] polymerase SRO3 [Benincasa hispida][more]
KAA0065063.17.2e-22484.57putative inactive poly [Cucumis melo var. makuwa][more]
XP_008444977.17.2e-22484.57PREDICTED: probable inactive poly [ADP-ribose] polymerase SRO3 [Cucumis melo][more]
XP_004148400.16.1e-22385.14probable inactive poly [ADP-ribose] polymerase SRO3 [Cucumis sativus] >KGN62785.... [more]
XP_023537456.12.0e-21080.21probable inactive poly [ADP-ribose] polymerase SRO3 [Cucurbita pepo subsp. pepo]... [more]
Match NameE-valueIdentityDescription
AT1G32230.11.7e-4529.07WWE protein-protein interaction domain protein family [more]
AT1G32230.21.7e-4529.07WWE protein-protein interaction domain protein family [more]
AT1G32230.31.7e-4529.07WWE protein-protein interaction domain protein family [more]
AT2G35510.12.2e-4528.95similar to RCD one 1 [more]
AT1G70440.11.6e-3532.33similar to RCD one 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPFAMPF00644PARPcoord: 261..348
e-value: 2.2E-7
score: 30.6
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPROSITEPS51059PARP_CATALYTICcoord: 195..414
score: 22.098661
IPR022003RST domainPFAMPF12174RSTcoord: 414..469
e-value: 1.4E-11
score: 44.1
IPR022003RST domainPROSITEPS51879RSTcoord: 404..473
score: 23.0919
IPR037197WWE domain superfamilyGENE3D3.30.720.50coord: 54..123
e-value: 2.6E-5
score: 26.0
IPR037197WWE domain superfamilySUPERFAMILY117839WWE domaincoord: 63..121
NoneNo IPR availableGENE3D3.90.228.10coord: 205..395
e-value: 2.3E-30
score: 107.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 22..43
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 23..43
NoneNo IPR availablePANTHERPTHR32263:SF19OS03G0230300 PROTEINcoord: 44..469
NoneNo IPR availableSUPERFAMILY56399ADP-ribosylationcoord: 209..395
IPR044964Inactive poly [ADP-ribose] polymerase RCD1/SRO1-5PANTHERPTHR32263INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO4-RELATEDcoord: 44..469
IPR004170WWE domainPROSITEPS50918WWEcoord: 48..123
score: 11.519839

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G010570.1Lsi08G010570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003950 NAD+ ADP-ribosyltransferase activity