Lsi08G010430 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G010430
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 8
Locationchr08: 19006299 .. 19012685 (-)
RNA-Seq ExpressionLsi08G010430
SyntenyLsi08G010430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATAAAAATCCAACGTGTAAGAAAATTATTCCTTTCTTCTCGTAAATTCCCGGAATCCAGCTAAAAAGAAAAAAGAAATTTCATTCGAATAATCATCCCCTTTTTTCTGTCATTAATTTAAATTAAATTTCTCCTAAATTGATGTTAAAAAGTCATCCCTATTTTGTTACAGTTTCACATCTTCAAAGACTGCTTTTAGAAGAAGCTCAATAAAGAAGAAGAACCAAATTCCTTTCACAAACATAGCAAAAGGATAAATCAAATCACCAGCTTCGAAGTTTTCGTTCTATGTACTTTCCATGTTTGCAGGCCGAAACGCTTCTGCCGAAGACGGTGTGCAAATGCTGAGATCGAGTTCTGGAGATTAGGATTTTTCCTGCCGGAAGCGGAGGAGATTGACGGCGGAGATGGTGGTTAGAAAAGTCGGCAAATACGAGGTCGGGAGGACGATTGGGGAAGGTACTTTCGCAAAGGTAAAGTTTGCTCAGAACACTGAGACTGGCGAGAGTTTTGCTATGAAAGTCCTCGATCGGAGCACGATAATCAAGCACAAGATGGTCGACCAGGTCTGTGTCTTCTCTATAATCTTTGTTTAAGAAATGGAAATATGTTCAGTCTCAGTCTTAACGGACAATTAGAGTGAAATAGAGAGAATTCGTCTATGTAATTTGCTTTATATGACCACATCATTTCTGCACCGCAAAATGGTTTCATTACTGCTGCTTTCAATTGAAGCCAAGATGAAATTAACTTTGTGTAAAGTTGAAATGTTTCAGAGTTAGCCATCAAAACAGTATTGGCTGTGATTACATGCTTCAATGATGTCGGTTGTTGAATTAGATGGTATATAGTATCATAGTGAATGTATTTGGTATCGATTGAGCTTCATTTGTTATCATCCTTGGGCTTCTCTAACGACAATCAATTGAAGAATTAAAACCTTCTTTATTTCTCACCTACTCGCTTTCACTCACTGAGTTGAAGAAGAGGAAAAGGAACTTTCGATGTCTTGGACTTCGAATGAAGGGAAATGATATGTGGCTGCTTTATACTGTTTGATTAAAGCTGCAAATATTAGCGGATGGGGAATCTTACTGTTACTGATACAAACTGTAGCTTCTGTACCTTTTCTCTTGAACAGTGCAAAAAATTGGTTGGTGATGACGGAGTTGTATGAACAAATTCATACACGGATGCTATCTTTCAAAAATAATGTAACTGGCAGGTTGTTTGATGGGTACTTGATTGAGCCAGACATTTTTATATCATAAGCTTCTAAATGTCTGTGTTTCAAGTTGTCCTATCCTACCAATGTTCTTCGTATGTCTCTTTTTGCATGCTCGATCCTCAGTCTTCTACTGATTAGCTTTGTAGTTCTTGTAAACTAATTGGAATGCAATCTGAAGCTTTTTTCAATATTTGATCCTGTAAAGTGTCGAGCATTTCGTCCAACAACTGTTACTAATATTCTGCAGATAAAGAAGGAGATTTCTATCATGAAGCTTGTGAGGCACCCTTATGTTGTTCGTCTTCATGAGGCATGGATTAATTTCTGAAGTATTTATTCTCAATTTATAATTTTTAGTCTCTTTTCAAGAGCTTAAGTTTGAACTTCCTTTTTCTTGTTTAACATTGGATACGGTATCTATTTTGGTTTCTAGGTTCTTGCAAGCCGTACTAAGATTTACATAATCCTGGAGTACATTACTGGTGGTGAATTGTTTGACAAAATAGTGAGTAATTCAAAAAACAGAGCATTGTTTAAGAAAGATGAGCCATTTCAAAATTTGGAACTATTTACTATTTTGTATCCCTATAGTTTAATGATGTAATCCATTCCATACCCTGCAGGTTCATCATGGACGGCTAAGTGAAGCTGAATCTAGGAAGTACTTCCAACAGCTAATTGATGGTGTAGATTATTGCCACAGCAAGGGAGTATACCACAGAGATTTGAAGGTATGGCTGTTTTTTATTGGCTGAGATCGTGGAGAAAATATTCTAATTGTGGTTTGGAAAAGAAAAATAAGAATCTGATCATTTTTTTTTTTATCTTTCTTTCTCTGAACAGCCTGAGAATCTCCTACTCGACTCCCTAGGTAATTTAAGGATCTCAGACTTTGGTCTGAGTGCATTGCCTGAACAAGTAAGACATATTTTAATGATTTTTATTTACTTGTAGATTTACACTTCTTGCCTGAACAAGTAGATATAGACTTCTTGCGTAATTTTAAGAGACACAACAAATAGCTGATTGTATTTGCTGACAGGGAGTGAGCCTACTCCGCACAACATGTGGGACTCCAAACTATGTAGCACCAGAGGTATTTATGTTGGTATCTAAAAGCTTGTTAAACCAAAATTTTATAAATTGATAATGACAGTTATAACTTATAAGCTTGCCTTTTGGTTCGATTCTCAATCACTTCATCTCTCATGTAGGTGCTCAATCACAAGGGTTATGATGGTGCTATGGCAGATGTTTGGTCCTGTGGGGTAATCCTTTATGTGTTGATGGCAGGATATCTTCCATTTGATGAGCTTGATCTCACCACGTTATATAGTAAGGCATGAACATTTTGTCATGATATCTTAAATCTACGTCACCTTTTTATGTTGCATTTTCCATTCAAACGCTGGAATGCTATCAACTTCTTTCTTGGGTGCCAAATGCGATCAATGCTACAACGTGAGGAGTGATTTTTTTTTTTTTTTTTTTGTTGAAACAAGAAAAAAGTTTCCACTCTGTAAGCACAGGCAGTCAAAGCAGTTAAACTATAGAACAATCTATTTGTCATATCTCTTTCAGTCGAATTTAAATCAGGATGTGAGGGCCTTTTATAGGTTCTTTATTTTTAAGCTTTTGATCGTAATCTTCCTAGGTTAACTTAAACTAAAATATCATTTCAACATGCATTTGAATGAATGCCTAATATTGTCATTTGCCTTTCAACAGATTGAGAGAGCTGACTTTTCATGTCCATCTTGGTTCCCAGTGGGGGCAAAAGCTTTAATCCACAGAATATTTGACCCTAATCCTTCAACTGTAAGTATGGATTGCCTGACATGACATGCCGTTGAACATTCTTTATGCCTTCAACTTACTGTCTAAATACAAAGAAAAAAAAAGAAAGAAAGAAACAGTTCATGGTATTTGCCAAGTAAAAAAAATAACAATTTACTGTCAAAGCATGCATTTCTTTTCCCAAAATCTGAGTGATGGATCGAATTACCTGAGACCTCAATGGATTTTGTTAGATTCCAGCACTGCAAAGTTATAACGTCCTTTAAGTTTCATACCACATCATTACTTAAGGAAGCCAATGTAGTAGTAACTCATGTTAGCGCTGTTTTTTAACACTCTTATGAACTGAAATTTTTCATTATTCTCAACATTATACGTACCACAGAGTAATTGTTGCTATCAATCTCATTATTTTTGGGTATTAGTGCTTTCAGACGCAAGGATTAAAATATTGATGTTGGTATCCATATCAAAATTTCAATTACGGATATATCGGATGTCAACGGATATTTTTGTAAATAAAAAAATTTATAAAGTAATTAATAATTAAGTTGTTTTTACTTCTAAACAAAGTTATAGAGTTTGTTATTGGTATTTATATTTATTTTAAGGTTAGTAGATGATATTTTTTTATGTTTTATGGGTATTTATAACAGATATTGGAGATATTTGATTTATCTCTAGTATCGATGTTGAACCCTCTGATTTATGGATATATCGATATATTGACAGATATTTTAATCCTTATTGATAAGTTGCTCTCTTTTCTTCTGTGTAGCGTATTACAATTGAACAGATCAAAGAGGATGAGTGGTTCAAGAAAAATTATGTTTCTGCCAACCTGTTTGAAGATGAAGATGTAAATCTGGATGATGTAAATGCTGTCTTTGATGATCCTGAGGTTGGTAAGGATTTGAGTACTATATTGGCATATATGTGAACTTGGATTTATTGAACTCTAGGTATTGTTAGGTTTGAAGATTAGTTACGGATTCTATTCTTATTTTGATCTCCTTCGTTGGCAACCTTTCAACTAACTGTAACTTTGGTTCCTATCCATTAATTACTTTCAAGCAAATACATAATAAAAATGGTGAATTTACTATTCTAGGTTAAATGTTTCCAGCCAGCCTGTTCTCTACCAGCATAGTTTCCCCACTATTGAGACATAAAAGCTCACATTGTTTTGTGCTAGCACACATTTTTATGTGTTTGTCTTCTTTCAATGATTTTTTCCCTTTTCTTTTGGTCAATATTTTTGCCTACGCTAAGGTGGTTAGACTTTATAAATTTTAGTCTCTAGAGACAATTTTTATGTAGATACTAAAAGTTGTAAATGTGGTTGAAGGTGGTGGCGTCGAAGTGTGGTGTTCAATTTGAGATCGATTTATAAAATTGTTGTGGTTATTATTAGCTACTTCTCCTGACTCGCGAAATTTGTCCATAGGAACAAAGGACCGAAGAACAACGTGGTAACGAGGATATGAGCCCTCTGGCCCTTAATGCATTTGATTTAATAATTCTATCTCAAGGTTTAAACCTGAGAACGATGTTTGACCGTGGCCAGGTATGTTTGTGTTTTTATTAACTTTCCCTATTTTCATATAAAATATGTAGCTAAATAGAGAGCCAATTTGCCTAAAAAAATAAAAGACCTGATGTGAAGCTACTACAAAATTGTTAATTTGGTGTATGATTGTGTTCCAACTCAACATTAAGCATATATGGTCATTTTCTCAGACTGACAAATATCAGTTCTGATCATTTCAAAATGTGTTTGTTTAAGATAGCTTAATATGTGTATATTGCTCGAGAATTGATGGTTGAATTTCTTCTGATCATGGTAGGATTCTATGAAATACCCGACACGCTTCGTCTGCCAAAAGCCTGCAAAGGTTTTATTGTCAACAATGGAAGTTGTAGCTCAATCAATGGGTTACAAGACACACATTCGCAATTACAAGGTAAAAGATTTGTGTTAGTCATTTGGTTAGATCTCATCTGAATTCTTGCGATTCAATTTTTTTAGGCTCCTCCCTTTCTATCTAATCCTTGTATACAAAGGATTCCATAATGATTTTCCAATTCTAAGTCATAAAGTTTAAAAAATTAATTAATTGATTTTTGAAATAAAAATTTGAAAGTTTATGTCTAAAAAAACTCTAAAGACCCATACATTCAATTAGATTCAACAAAATTGAAAAATCCAAGTATCTATTGAAAAAAAATTTGAAAGTTTAAATACCTCTATGATACATTTTAAAGTTGAATGATGTATTAGACTCTATTTTTTTTATAAAAAAAAAATACAAAATTGAAATTTTGAAGACCTATTAGACATTTTTTAAAAGTTCTTGTAATTTATCTTAATCTTAATCAAAATTCTGGTGAATGTCAGATGAGAGTAGAAGGCCCATCGGCAAGCAAAACTTCGTATTTTTCAGTCATTATGGAAGTAAGTTTCACCATTTTCCTGCATGAAAAGTTGTACAAAATTTCTATATTATGTTCAAGGACTTTGATCCGAATCATTTTTCCCGTAGATTTTTGAAGTTGCTCCCACGTTCTTTATGGTGGACATTCAGAAAGCAGCAGGGGAAACCAGCGAGTACCTCAAGGTAGGTCATGGTAGAATTTCCATCTTGTGTCGATCGATATTCGCCACTTAAAGAGTATACCACATTTCTCCTCATTCCCATCCATATCTCTCATTGTACTTCATAGATATTGAAACAATGCCATTATGTATCGCAGTTCTACAAGAGCTTTTATGGCAAACTTGAAGATATCATCTGGAAACCTTCAATTGACACCAGCAAATCAAGGATCACCAAGAACAAGAGTAAAAAACGTTGATGCAACAAAGCCTGAGAAACTTCACAGCGTCATCTAACCAGTGGAAGAAGCTTCATCTAACCAGTGGAAGAAGATCGAAGCAGTATGATGTTGAGATATGTTTACAGTTCTTTAATTAATTATACCTTGTCTGTTTTTTGGCCAATAGTGAGAAGATAAGAAATCCTAGGTTATTTGTATAAGAATATTTGTATATACTATCATCTAGTTTTTATTAAAATCTCTTTGCAAATTTGCAGACCGACGCCTCGCCATTCTTTCGCTAAATAGAGAAGAAACAATGGCGTCGACATTAAATTGTCTTGGTATTTGTCTGAAGCTCAATATCAGCTGGTTATTCTCCTGATCTTACAGAAATTAGCTTCATATGAATATTTTGAGTTGAATGATATTAGGTTTGTAAAGAATTTAAGATTGTATCTCACCAACCACACTAAAGAAAATCTCCGAAACCTCCACAAGAACAGTATCCGTTCAAAAACTTATGAAGTTGCTTACTATCTC

mRNA sequence

AATATAAAAATCCAACGTGTAAGAAAATTATTCCTTTCTTCTCGTAAATTCCCGGAATCCAGCTAAAAAGAAAAAAGAAATTTCATTCGAATAATCATCCCCTTTTTTCTGTCATTAATTTAAATTAAATTTCTCCTAAATTGATGTTAAAAAGTCATCCCTATTTTGTTACAGTTTCACATCTTCAAAGACTGCTTTTAGAAGAAGCTCAATAAAGAAGAAGAACCAAATTCCTTTCACAAACATAGCAAAAGGATAAATCAAATCACCAGCTTCGAAGTTTTCGTTCTATGTACTTTCCATGTTTGCAGGCCGAAACGCTTCTGCCGAAGACGGTGTGCAAATGCTGAGATCGAGTTCTGGAGATTAGGATTTTTCCTGCCGGAAGCGGAGGAGATTGACGGCGGAGATGGTGGTTAGAAAAGTCGGCAAATACGAGGTCGGGAGGACGATTGGGGAAGGTACTTTCGCAAAGGTAAAGTTTGCTCAGAACACTGAGACTGGCGAGAGTTTTGCTATGAAAGTCCTCGATCGGAGCACGATAATCAAGCACAAGATGGTCGACCAGGTTCTTGCAAGCCGTACTAAGATTTACATAATCCTGGAGTACATTACTGGTGGTGAATTGTTTGACAAAATAGTTCATCATGGACGGCTAAGTGAAGCTGAATCTAGGAAGTACTTCCAACAGCTAATTGATGGTGTAGATTATTGCCACAGCAAGGGAGTATACCACAGAGATTTGAAGCCTGAGAATCTCCTACTCGACTCCCTAGGTAATTTAAGGATCTCAGACTTTGGTCTGAGTGCATTGCCTGAACAAGGAGTGAGCCTACTCCGCACAACATGTGGGACTCCAAACTATGTAGCACCAGAGGTGCTCAATCACAAGGGTTATGATGGTGCTATGGCAGATGTTTGGTCCTGTGGGATTGAGAGAGCTGACTTTTCATGTCCATCTTGGTTCCCAGTGGGGGCAAAAGCTTTAATCCACAGAATATTTGACCCTAATCCTTCAACTGTAAAGGATGAGTGGTTCAAGAAAAATTATGTTTCTGCCAACCTGTTTGAAGATGAAGATGTAAATCTGGATGATGTAAATGCTGTCTTTGATGATCCTGAGGAACAAAGGACCGAAGAACAACGTGGTAACGAGGATATGAGCCCTCTGGCCCTTAATGCATTTGATTTAATAATTCTATCTCAAGGTTTAAACCTGAGAACGATGTTTGACCGTGGCCAGGATTCTATGAAATACCCGACACGCTTCGTCTGCCAAAAGCCTGCAAAGGTTTTATTGTCAACAATGGAAGTTGTAGCTCAATCAATGGGTTACAAGACACACATTCGCAATTACAAGGACTTTGATCCGAATCATTTTTCCCGTAGATTTTTGAAGTTGCTCCCACGTTCTTTATGGTGGACATTCAGAAAGCAGCAGGGGAAACCAGCGAGTACCTCAAGTTCTACAAGAGCTTTTATGGCAAACTTGAAGATATCATCTGGAAACCTTCAATTGACACCAGCAAATCAAGGATCACCAAGAACAAGAGTAAAAAACGTTGATGCAACAAAGCCTGAGAAACTTCACAGCGTCATCTAACCAGTGGAAGAAGCTTCATCTAACCAGTGGAAGAAGATCGAAGCAACCGACGCCTCGCCATTCTTTCGCTAAATAGAGAAGAAACAATGGCGTCGACATTAAATTGTCTTGGTATTTGTCTGAAGCTCAATATCAGCTGGTTATTCTCCTGATCTTACAGAAATTAGCTTCATATGAATATTTTGAGTTGAATGATATTAGGTTTGTAAAGAATTTAAGATTGTATCTCACCAACCACACTAAAGAAAATCTCCGAAACCTCCACAAGAACAGTATCCGTTCAAAAACTTATGAAGTTGCTTACTATCTC

Coding sequence (CDS)

ATGGTGGTTAGAAAAGTCGGCAAATACGAGGTCGGGAGGACGATTGGGGAAGGTACTTTCGCAAAGGTAAAGTTTGCTCAGAACACTGAGACTGGCGAGAGTTTTGCTATGAAAGTCCTCGATCGGAGCACGATAATCAAGCACAAGATGGTCGACCAGGTTCTTGCAAGCCGTACTAAGATTTACATAATCCTGGAGTACATTACTGGTGGTGAATTGTTTGACAAAATAGTTCATCATGGACGGCTAAGTGAAGCTGAATCTAGGAAGTACTTCCAACAGCTAATTGATGGTGTAGATTATTGCCACAGCAAGGGAGTATACCACAGAGATTTGAAGCCTGAGAATCTCCTACTCGACTCCCTAGGTAATTTAAGGATCTCAGACTTTGGTCTGAGTGCATTGCCTGAACAAGGAGTGAGCCTACTCCGCACAACATGTGGGACTCCAAACTATGTAGCACCAGAGGTGCTCAATCACAAGGGTTATGATGGTGCTATGGCAGATGTTTGGTCCTGTGGGATTGAGAGAGCTGACTTTTCATGTCCATCTTGGTTCCCAGTGGGGGCAAAAGCTTTAATCCACAGAATATTTGACCCTAATCCTTCAACTGTAAAGGATGAGTGGTTCAAGAAAAATTATGTTTCTGCCAACCTGTTTGAAGATGAAGATGTAAATCTGGATGATGTAAATGCTGTCTTTGATGATCCTGAGGAACAAAGGACCGAAGAACAACGTGGTAACGAGGATATGAGCCCTCTGGCCCTTAATGCATTTGATTTAATAATTCTATCTCAAGGTTTAAACCTGAGAACGATGTTTGACCGTGGCCAGGATTCTATGAAATACCCGACACGCTTCGTCTGCCAAAAGCCTGCAAAGGTTTTATTGTCAACAATGGAAGTTGTAGCTCAATCAATGGGTTACAAGACACACATTCGCAATTACAAGGACTTTGATCCGAATCATTTTTCCCGTAGATTTTTGAAGTTGCTCCCACGTTCTTTATGGTGGACATTCAGAAAGCAGCAGGGGAAACCAGCGAGTACCTCAAGTTCTACAAGAGCTTTTATGGCAAACTTGAAGATATCATCTGGAAACCTTCAATTGACACCAGCAAATCAAGGATCACCAAGAACAAGAGTAAAAAACGTTGATGCAACAAAGCCTGAGAAACTTCACAGCGTCATCTAA

Protein sequence

MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVDYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLNHKGYDGAMADVWSCGIERADFSCPSWFPVGAKALIHRIFDPNPSTVKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGNEDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMGYKTHIRNYKDFDPNHFSRRFLKLLPRSLWWTFRKQQGKPASTSSSTRAFMANLKISSGNLQLTPANQGSPRTRVKNVDATKPEKLHSVI
Homology
BLAST of Lsi08G010430 vs. ExPASy Swiss-Prot
Match: Q9STV4 (CBL-interacting serine/threonine-protein kinase 8 OS=Arabidopsis thaliana OX=3702 GN=CIPK8 PE=1 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 7.6e-137
Identity = 253/369 (68.56%), Postives = 284/369 (76.96%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYE+GRTIGEGTFAKVKFAQNTETGES AMK++DRSTIIK KMVDQ       
Sbjct: 1   MVVRKVGKYELGRTIGEGTFAKVKFAQNTETGESVAMKIVDRSTIIKRKMVDQIKREISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIV +GRLSE+E+RKYF QLIDGV
Sbjct: 61  MKLVRHPCVVRLYEVLASRTKIYIILEYITGGELFDKIVRNGRLSESEARKYFHQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDS GNL+ISDFGLSALPEQGV++L+TTCGTPNYVAPEVL+
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSQGNLKISDFGLSALPEQGVTILKTTCGTPNYVAPEVLS 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGY+GA+AD+WSCG                        I++A+FSCPS+F +GAK+LI+
Sbjct: 181 HKGYNGAVADIWSCGVILYVLMAGYLPFDEMDLPTLYSKIDKAEFSCPSYFALGAKSLIN 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RI DPNP T        KDEWF K+Y    L + E VNLDDV A FDDPEEQ T  Q G 
Sbjct: 241 RILDPNPETRITIAEIRKDEWFLKDYTPVQLIDYEHVNLDDVYAAFDDPEEQ-TYAQDGT 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
            D  PL LNAFDLIILSQGLNL T+FDRG+DSMK+ TRF+  KPA V+LS+MEVV+QSMG
Sbjct: 301 RDTGPLTLNAFDLIILSQGLNLATLFDRGKDSMKHQTRFISHKPANVVLSSMEVVSQSMG 360

BLAST of Lsi08G010430 vs. ExPASy Swiss-Prot
Match: Q5JLD8 (CBL-interacting protein kinase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK8 PE=2 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.7e-128
Identity = 238/367 (64.85%), Postives = 271/367 (73.84%), Query Frame = 0

Query: 3   VRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ--------- 62
           +R+VGKYEVGRTIGEGTFAKVKFAQNTE+GES AMKV+DRS+I+KHKM DQ         
Sbjct: 7   LRRVGKYEVGRTIGEGTFAKVKFAQNTESGESVAMKVVDRSSILKHKMADQIKREISIMK 66

Query: 63  ------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVDY 122
                       VLASR KI+IILE+ITGGELFDKI+ HGRL+EA++R+YFQQLIDGVD+
Sbjct: 67  LVRHPNVVRLHEVLASRKKIFIILEFITGGELFDKIIRHGRLNEADARRYFQQLIDGVDF 126

Query: 123 CHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLNHK 182
           CHSKGVYHRDLKPENLLLDS GNL+ISDFGLSA P QG +LLRTTCGTPNYVAPEVL+HK
Sbjct: 127 CHSKGVYHRDLKPENLLLDSQGNLKISDFGLSAWPAQGGALLRTTCGTPNYVAPEVLSHK 186

Query: 183 GYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIHRI 242
           GYDGA+AD WSCG                        IE A++S P+WFP GAK+LIHRI
Sbjct: 187 GYDGALADTWSCGVILYVLLAGYLPFDEVDLTTLYGKIESAEYSFPAWFPNGAKSLIHRI 246

Query: 243 FDPNP-------STVKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGNED 302
            DPNP           DEWFKKNY      E E+VNLDDVNA FDDPEE    +   +++
Sbjct: 247 LDPNPDKRIRIEEIRNDEWFKKNYEPTREIESEEVNLDDVNAAFDDPEED--ADHTLDDE 306

Query: 303 MSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMGYK 318
             PL LNAFDLIILSQGLNL  +FDR QD  K   RF+ +KPAKV++S+MEVVAQSMGYK
Sbjct: 307 AGPLTLNAFDLIILSQGLNLAALFDRRQDYDKLQNRFLSRKPAKVIMSSMEVVAQSMGYK 366

BLAST of Lsi08G010430 vs. ExPASy Swiss-Prot
Match: Q9LDI3 (CBL-interacting serine/threonine-protein kinase 24 OS=Arabidopsis thaliana OX=3702 GN=CIPK24 PE=1 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.4e-111
Identity = 204/367 (55.59%), Postives = 253/367 (68.94%), Query Frame = 0

Query: 3   VRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ--------- 62
           +R+VGKYEVGRTIGEGTFAKVKFA+NT+TG++ A+K++ +STI+K++MVDQ         
Sbjct: 5   MRRVGKYEVGRTIGEGTFAKVKFARNTDTGDNVAIKIMAKSTILKNRMVDQIKREISIMK 64

Query: 63  ------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVDY 122
                       VLAS +KIYI+LE++TGGELFD+IVH GRL E+ESRKYFQQL+D V +
Sbjct: 65  IVRHPNIVRLYEVLASPSKIYIVLEFVTGGELFDRIVHKGRLEESESRKYFQQLVDAVAH 124

Query: 123 CHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLNHK 182
           CH KGVYHRDLKPENLLLD+ GNL++SDFGLSALP++GV LLRTTCGTPNYVAPEVL+ +
Sbjct: 125 CHCKGVYHRDLKPENLLLDTNGNLKVSDFGLSALPQEGVELLRTTCGTPNYVAPEVLSGQ 184

Query: 183 GYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIHRI 242
           GYDG+ AD+WSCG                        I  A+FSCP WF    K LIHRI
Sbjct: 185 GYDGSAADIWSCGVILFVILAGYLPFSETDLPGLYRKINAAEFSCPPWFSAEVKFLIHRI 244

Query: 243 FDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGNED 302
            DPNP T        KD WF+ NYV     E+E+VNLDD+ AVFD  E     E     D
Sbjct: 245 LDPNPKTRIQIQGIKKDPWFRLNYVPIRAREEEEVNLDDIRAVFDGIEGSYVAENVERND 304

Query: 303 MSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMGYK 318
             PL +NAF++I LSQGLNL  +FDR QD +K  TRFV ++    +++ +E VA SMG+K
Sbjct: 305 EGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSRREPSEIIANIEAVANSMGFK 364

BLAST of Lsi08G010430 vs. ExPASy Swiss-Prot
Match: Q69Q47 (CBL-interacting protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK24 PE=1 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.1e-110
Identity = 205/366 (56.01%), Postives = 252/366 (68.85%), Query Frame = 0

Query: 4   RKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ---------- 63
           ++VG+YEVGRTIG+GTFAKVKFA + +TG + AMKVLD+ TI+ H+M+ Q          
Sbjct: 13  KRVGRYEVGRTIGQGTFAKVKFAVDADTGAAVAMKVLDKDTILNHRMLHQIKREISIMKI 72

Query: 64  -----------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVDYC 123
                      VLA +TKIYIILE ITGGELFDKI   G+L E E+RKYFQQLID ++YC
Sbjct: 73  VRHPNIVRLNEVLAGKTKIYIILELITGGELFDKIARQGKLRENEARKYFQQLIDAINYC 132

Query: 124 HSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLNHKG 183
           HSKGVYHRDLKPENLLLDS GNL++SDFGLS L ++GV LL TTCGTPNYVAPEVL++ G
Sbjct: 133 HSKGVYHRDLKPENLLLDSRGNLKVSDFGLSTLAQKGVGLLHTTCGTPNYVAPEVLSNNG 192

Query: 184 YDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIHRIF 243
           YDG+ ADVWSCG                        I    FSCP WF  GA +LIHRI 
Sbjct: 193 YDGSAADVWSCGVILYVLMAGYLPFEEDDLPTLYDKITAGQFSCPYWFSPGATSLIHRIL 252

Query: 244 DPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGNEDM 303
           DPNP T        +D WFKK YV+    EDE+V+LDDV AVFD+ E++   EQ  + D 
Sbjct: 253 DPNPKTRITIEQIREDTWFKKTYVAIKRGEDENVDLDDVQAVFDNIEDKYVSEQVTHNDG 312

Query: 304 SPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMGYKT 318
            PL +NAF++I LSQGL+L  +FDR Q+ +K  TRFV +KPAK +++T+EVVA++MG K 
Sbjct: 313 GPLVMNAFEMITLSQGLDLSALFDRQQEFVKRQTRFVSRKPAKTIVATIEVVAETMGLKV 372

BLAST of Lsi08G010430 vs. ExPASy Swiss-Prot
Match: Q6ZLP5 (CBL-interacting protein kinase 23 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK23 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 8.2e-99
Identity = 191/368 (51.90%), Postives = 243/368 (66.03%), Query Frame = 0

Query: 5   KVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ----------- 64
           +VG+YE+GRT+GEGTFAKVKFA+N ++GE+ A+K+LD+  ++KHKM+ Q           
Sbjct: 9   RVGRYELGRTLGEGTFAKVKFARNADSGENVAIKILDKDKVLKHKMIAQIKREISTMKLI 68

Query: 65  ----------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVDYCH 124
                     V+AS+TKIYI++E +TGGELFDKI   GRL E ++RKYFQQLI+ VDYCH
Sbjct: 69  RHPNVIRMHEVMASKTKIYIVMELVTGGELFDKIASRGRLKEDDARKYFQQLINAVDYCH 128

Query: 125 SKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQ--GVSLLRTTCGTPNYVAPEVLNHK 184
           S+GVYHRDLKPENLLLD+ G L++SDFGLSAL +Q     LL TTCGTPNYVAPEV+N+K
Sbjct: 129 SRGVYHRDLKPENLLLDASGTLKVSDFGLSALSQQVREDGLLHTTCGTPNYVAPEVINNK 188

Query: 185 GYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIHRI 244
           GYDGA AD+WSCG                        I +ADFSCPSWF   AK LI +I
Sbjct: 189 GYDGAKADLWSCGVILFVLMAGYLPFEDSNLMSLYKKIFKADFSCPSWFSTSAKKLIKKI 248

Query: 245 FDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGNED 304
            DPNPST       + +EWFKK Y     FE  DVNLDD+N++F++  +Q T+      +
Sbjct: 249 LDPNPSTRITIAELINNEWFKKGYQPPR-FETADVNLDDINSIFNESGDQ-TQLVVERRE 308

Query: 305 MSPLALNAFDLIILSQGLNLRTMFDR-GQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMGY 318
             P  +NAF+LI  SQGLNL T+F++  Q S+K  TRF  + PA  +LS +E  A  MG+
Sbjct: 309 ERPSVMNAFELISTSQGLNLGTLFEKQSQGSVKRETRFASRLPANEILSKIEAAAGPMGF 368

BLAST of Lsi08G010430 vs. ExPASy TrEMBL
Match: A0A6J1KP02 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111495959 PE=3 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 6.4e-163
Identity = 301/369 (81.57%), Postives = 308/369 (83.47%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETG S AMKVLDRSTIIKHKMV+Q       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGGSVAMKVLDRSTIIKHKMVEQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESR+YFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRRYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERADFSCPSWFPVGAKALIH
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERADFSCPSWFPVGAKALIH 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        +DEWF KNYV    FEDEDVNLDDVNAVFDDPEEQRTEE+RGN
Sbjct: 241 RIFDPNPSTRITIEQIREDEWFMKNYVLVKHFEDEDVNLDDVNAVFDDPEEQRTEEERGN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           EDMSPLALNAFDLIILSQGLNL TMFDRGQD+MKYPTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 EDMSPLALNAFDLIILSQGLNLGTMFDRGQDTMKYPTRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. ExPASy TrEMBL
Match: A0A6J1GI84 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111454163 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.4e-162
Identity = 300/369 (81.30%), Postives = 309/369 (83.74%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETG S AMKVLDRSTIIKHKMV+Q       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGGSVAMKVLDRSTIIKHKMVEQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESR+YFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRRYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERA+FSCPSWFPVGAKALIH
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERAEFSCPSWFPVGAKALIH 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        +DEWF KNYVS   FEDEDVNLDDVNAVFDDPEEQRTEE+RGN
Sbjct: 241 RIFDPNPSTRITIEQIREDEWFMKNYVSVKHFEDEDVNLDDVNAVFDDPEEQRTEEERGN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           EDMSPLALNAFDLIILSQGLNL TMFDRGQD+MK+PTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 EDMSPLALNAFDLIILSQGLNLGTMFDRGQDTMKHPTRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. ExPASy TrEMBL
Match: A0A1S3BCG3 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103488146 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 2.1e-161
Identity = 301/369 (81.57%), Postives = 307/369 (83.20%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGES AMKVLDRSTIIKHKMV+Q       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESVAMKVLDRSTIIKHKMVEQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIV HGRLSEAESRKYFQQLIDGV
Sbjct: 61  MKLVRHPYIVRLHEVLASRTKIYIILEYITGGELFDKIVRHGRLSEAESRKYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERADFSCPSWFPVGAKALI 
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERADFSCPSWFPVGAKALIC 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        ++EWFKKNYVS  L EDEDVNLDDVNAVFDDPEE RTEEQ GN
Sbjct: 241 RIFDPNPSTRITIEQIRENEWFKKNYVSVKLVEDEDVNLDDVNAVFDDPEEPRTEEQSGN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           E+MSPLALNAFDLIILSQGLNL TMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 ENMSPLALNAFDLIILSQGLNLGTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. ExPASy TrEMBL
Match: A0A0A0LPN8 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2G372190 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 4.6e-161
Identity = 300/369 (81.30%), Postives = 306/369 (82.93%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGES AMKVLDRSTIIKHKMV+Q       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESVAMKVLDRSTIIKHKMVEQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIV HGRLSEAESRKYFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVRHGRLSEAESRKYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHS+GVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSRGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERADFSCPSWFPVGAKALI 
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERADFSCPSWFPVGAKALIC 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        +DEWFKKNYVS  L EDEDVNLDDVNAVFDDPEE RTEEQR N
Sbjct: 241 RIFDPNPSTRITIEQIREDEWFKKNYVSVKLVEDEDVNLDDVNAVFDDPEEPRTEEQRSN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           E+M PLALNAFDLIILSQGLNL TMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 ENMCPLALNAFDLIILSQGLNLGTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. ExPASy TrEMBL
Match: A0A6J1BTR1 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111004720 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 3.1e-157
Identity = 291/369 (78.86%), Postives = 301/369 (81.57%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGES AMKVLDRSTIIKHKMVDQ       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESVAMKVLDRSTIIKHKMVDQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCH KGVYHRDLKPENLLLDS GNL+ISDFGLSA+PEQG SLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHIKGVYHRDLKPENLLLDSQGNLKISDFGLSAMPEQGGSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERADFSCPSWFPVGAKALIH
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERADFSCPSWFPVGAKALIH 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        ++EWFKKNY    L E EDVNLDDVNAVFDDPEE+RTE++ G 
Sbjct: 241 RIFDPNPSTRITIEKIREEEWFKKNYAPVKLVEYEDVNLDDVNAVFDDPEEERTEDRHGI 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           +DMSPL LNAFDLIILSQGLNL TMFDRGQDSMKYPTRF+CQKPA VLLSTMEVVAQSMG
Sbjct: 301 KDMSPLTLNAFDLIILSQGLNLGTMFDRGQDSMKYPTRFICQKPANVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. NCBI nr
Match: XP_038885498.1 (CBL-interacting serine/threonine-protein kinase 8 [Benincasa hispida])

HSP 1 Score: 595.1 bits (1533), Expect = 4.4e-166
Identity = 307/369 (83.20%), Postives = 311/369 (84.28%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGES AMK+LDRSTIIKHKMVDQ       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESVAMKILDRSTIIKHKMVDQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERA+FSCPSWFPVGAKALIH
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERAEFSCPSWFPVGAKALIH 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           +IFDPNPST        +DEWFKKNYVS  LFEDEDVNLDDVNAVFDDPEEQRTEEQRGN
Sbjct: 241 KIFDPNPSTRITIEQIREDEWFKKNYVSVKLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           EDMSPLALNAFDLIILSQGLNL TMFDRGQDSMKY TRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 EDMSPLALNAFDLIILSQGLNLGTMFDRGQDSMKYATRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. NCBI nr
Match: XP_023538368.1 (CBL-interacting serine/threonine-protein kinase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 584.3 bits (1505), Expect = 7.7e-163
Identity = 301/369 (81.57%), Postives = 309/369 (83.74%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETG S AMKVLDRSTIIKHKMV+Q       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGGSVAMKVLDRSTIIKHKMVEQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESR+YFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRRYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERA+FSCPSWFPVGAKALIH
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERAEFSCPSWFPVGAKALIH 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        +DEWF KNYVS   FEDEDVNLDDVNAVFDDPEEQRTEE+RGN
Sbjct: 241 RIFDPNPSTRITIEQIREDEWFMKNYVSVKHFEDEDVNLDDVNAVFDDPEEQRTEEERGN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           EDMSPLALNAFDLIILSQGLNL TMFDRGQD+MKYPTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 EDMSPLALNAFDLIILSQGLNLGTMFDRGQDTMKYPTRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. NCBI nr
Match: XP_023001929.1 (CBL-interacting serine/threonine-protein kinase 8 [Cucurbita maxima])

HSP 1 Score: 583.6 bits (1503), Expect = 1.3e-162
Identity = 301/369 (81.57%), Postives = 308/369 (83.47%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETG S AMKVLDRSTIIKHKMV+Q       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGGSVAMKVLDRSTIIKHKMVEQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESR+YFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRRYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERADFSCPSWFPVGAKALIH
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERADFSCPSWFPVGAKALIH 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        +DEWF KNYV    FEDEDVNLDDVNAVFDDPEEQRTEE+RGN
Sbjct: 241 RIFDPNPSTRITIEQIREDEWFMKNYVLVKHFEDEDVNLDDVNAVFDDPEEQRTEEERGN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           EDMSPLALNAFDLIILSQGLNL TMFDRGQD+MKYPTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 EDMSPLALNAFDLIILSQGLNLGTMFDRGQDTMKYPTRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. NCBI nr
Match: KAG6585315.1 (CBL-interacting serine/threonine-protein kinase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 582.4 bits (1500), Expect = 2.9e-162
Identity = 300/369 (81.30%), Postives = 309/369 (83.74%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETG S AMKVLDRSTIIKHKMV+Q       
Sbjct: 65  MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGGSVAMKVLDRSTIIKHKMVEQIKKEISI 124

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESR+YFQQLIDGV
Sbjct: 125 MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRRYFQQLIDGV 184

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 185 DYCHSKGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 244

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERA+FSCPSWFPVGAKALIH
Sbjct: 245 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERAEFSCPSWFPVGAKALIH 304

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        +DEWF KNYVS   FEDEDVNLDDVNAVFDDPEEQRTEE+RGN
Sbjct: 305 RIFDPNPSTRITIEQIREDEWFMKNYVSVKHFEDEDVNLDDVNAVFDDPEEQRTEEERGN 364

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           EDMSPLALNAFDLIILSQGLNL TMFDRGQD+MK+PTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 365 EDMSPLALNAFDLIILSQGLNLGTMFDRGQDTMKHPTRFVCQKPAKVLLSTMEVVAQSMG 424

BLAST of Lsi08G010430 vs. NCBI nr
Match: XP_022951279.1 (CBL-interacting serine/threonine-protein kinase 8 [Cucurbita moschata] >KAG7020233.1 CBL-interacting serine/threonine-protein kinase 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 582.4 bits (1500), Expect = 2.9e-162
Identity = 300/369 (81.30%), Postives = 309/369 (83.74%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETG S AMKVLDRSTIIKHKMV+Q       
Sbjct: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGGSVAMKVLDRSTIIKHKMVEQIKKEISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESR+YFQQLIDGV
Sbjct: 61  MKLVRHPYVVRLHEVLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRRYFQQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDSLGNL+ISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSLGNLKISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGYDGAMADVWSCG                        IERA+FSCPSWFPVGAKALIH
Sbjct: 181 HKGYDGAMADVWSCGVILYVLMAGYLPFDELDLTTLYSKIERAEFSCPSWFPVGAKALIH 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RIFDPNPST        +DEWF KNYVS   FEDEDVNLDDVNAVFDDPEEQRTEE+RGN
Sbjct: 241 RIFDPNPSTRITIEQIREDEWFMKNYVSVKHFEDEDVNLDDVNAVFDDPEEQRTEEERGN 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
           EDMSPLALNAFDLIILSQGLNL TMFDRGQD+MK+PTRFVCQKPAKVLLSTMEVVAQSMG
Sbjct: 301 EDMSPLALNAFDLIILSQGLNLGTMFDRGQDTMKHPTRFVCQKPAKVLLSTMEVVAQSMG 360

BLAST of Lsi08G010430 vs. TAIR 10
Match: AT4G24400.2 (CBL-interacting protein kinase 8 )

HSP 1 Score: 492.3 bits (1266), Expect = 3.7e-139
Identity = 268/426 (62.91%), Postives = 306/426 (71.83%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYE+GRTIGEGTFAKVKFAQNTETGES AMK++DRSTIIK KMVDQ       
Sbjct: 1   MVVRKVGKYELGRTIGEGTFAKVKFAQNTETGESVAMKIVDRSTIIKRKMVDQIKREISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIV +GRLSE+E+RKYF QLIDGV
Sbjct: 61  MKLVRHPCVVRLYEVLASRTKIYIILEYITGGELFDKIVRNGRLSESEARKYFHQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDS GNL+ISDFGLSALPEQGV++L+TTCGTPNYVAPEVL+
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSQGNLKISDFGLSALPEQGVTILKTTCGTPNYVAPEVLS 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGY+GA+AD+WSCG                        I++A+FSCPS+F +GAK+LI+
Sbjct: 181 HKGYNGAVADIWSCGVILYVLMAGYLPFDEMDLPTLYSKIDKAEFSCPSYFALGAKSLIN 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RI DPNP T        KDEWF K+Y    L + E VNLDDV A FDDPEEQ T  Q G 
Sbjct: 241 RILDPNPETRITIAEIRKDEWFLKDYTPVQLIDYEHVNLDDVYAAFDDPEEQ-TYAQDGT 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 360
            D  PL LNAFDLIILSQGLNL T+FDRG+DSMK+ TRF+  KPA V+LS+MEVV+QSMG
Sbjct: 301 RDTGPLTLNAFDLIILSQGLNLATLFDRGKDSMKHQTRFISHKPANVVLSSMEVVSQSMG 360

Query: 361 YKTHIRNYK----DFDPNHFSRRFLKLLPRSLWWTFRKQQGKPASTSSSTRAFMANLKIS 371
           +KTHIRNYK        N  S  F  +L          +Q    +T SSTR F+ +L  S
Sbjct: 361 FKTHIRNYKMRVEGLSANKTS-HFSVIL----------EQETQKNTLSSTRLFVVSLMTS 414

BLAST of Lsi08G010430 vs. TAIR 10
Match: AT4G24400.1 (CBL-interacting protein kinase 8 )

HSP 1 Score: 488.4 bits (1256), Expect = 5.4e-138
Identity = 253/369 (68.56%), Postives = 284/369 (76.96%), Query Frame = 0

Query: 1   MVVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ------- 60
           MVVRKVGKYE+GRTIGEGTFAKVKFAQNTETGES AMK++DRSTIIK KMVDQ       
Sbjct: 1   MVVRKVGKYELGRTIGEGTFAKVKFAQNTETGESVAMKIVDRSTIIKRKMVDQIKREISI 60

Query: 61  --------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGV 120
                         VLASRTKIYIILEYITGGELFDKIV +GRLSE+E+RKYF QLIDGV
Sbjct: 61  MKLVRHPCVVRLYEVLASRTKIYIILEYITGGELFDKIVRNGRLSESEARKYFHQLIDGV 120

Query: 121 DYCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLN 180
           DYCHSKGVYHRDLKPENLLLDS GNL+ISDFGLSALPEQGV++L+TTCGTPNYVAPEVL+
Sbjct: 121 DYCHSKGVYHRDLKPENLLLDSQGNLKISDFGLSALPEQGVTILKTTCGTPNYVAPEVLS 180

Query: 181 HKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIH 240
           HKGY+GA+AD+WSCG                        I++A+FSCPS+F +GAK+LI+
Sbjct: 181 HKGYNGAVADIWSCGVILYVLMAGYLPFDEMDLPTLYSKIDKAEFSCPSYFALGAKSLIN 240

Query: 241 RIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGN 300
           RI DPNP T        KDEWF K+Y    L + E VNLDDV A FDDPEEQ T  Q G 
Sbjct: 241 RILDPNPETRITIAEIRKDEWFLKDYTPVQLIDYEHVNLDDVYAAFDDPEEQ-TYAQDGT 300

Query: 301 EDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMG 318
            D  PL LNAFDLIILSQGLNL T+FDRG+DSMK+ TRF+  KPA V+LS+MEVV+QSMG
Sbjct: 301 RDTGPLTLNAFDLIILSQGLNLATLFDRGKDSMKHQTRFISHKPANVVLSSMEVVSQSMG 360

BLAST of Lsi08G010430 vs. TAIR 10
Match: AT5G35410.1 (Protein kinase superfamily protein )

HSP 1 Score: 404.4 bits (1038), Expect = 1.0e-112
Identity = 204/367 (55.59%), Postives = 253/367 (68.94%), Query Frame = 0

Query: 3   VRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ--------- 62
           +R+VGKYEVGRTIGEGTFAKVKFA+NT+TG++ A+K++ +STI+K++MVDQ         
Sbjct: 5   MRRVGKYEVGRTIGEGTFAKVKFARNTDTGDNVAIKIMAKSTILKNRMVDQIKREISIMK 64

Query: 63  ------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVDY 122
                       VLAS +KIYI+LE++TGGELFD+IVH GRL E+ESRKYFQQL+D V +
Sbjct: 65  IVRHPNIVRLYEVLASPSKIYIVLEFVTGGELFDRIVHKGRLEESESRKYFQQLVDAVAH 124

Query: 123 CHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQGVSLLRTTCGTPNYVAPEVLNHK 182
           CH KGVYHRDLKPENLLLD+ GNL++SDFGLSALP++GV LLRTTCGTPNYVAPEVL+ +
Sbjct: 125 CHCKGVYHRDLKPENLLLDTNGNLKVSDFGLSALPQEGVELLRTTCGTPNYVAPEVLSGQ 184

Query: 183 GYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALIHRI 242
           GYDG+ AD+WSCG                        I  A+FSCP WF    K LIHRI
Sbjct: 185 GYDGSAADIWSCGVILFVILAGYLPFSETDLPGLYRKINAAEFSCPPWFSAEVKFLIHRI 244

Query: 243 FDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRGNED 302
            DPNP T        KD WF+ NYV     E+E+VNLDD+ AVFD  E     E     D
Sbjct: 245 LDPNPKTRIQIQGIKKDPWFRLNYVPIRAREEEEVNLDDIRAVFDGIEGSYVAENVERND 304

Query: 303 MSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSMGYK 318
             PL +NAF++I LSQGLNL  +FDR QD +K  TRFV ++    +++ +E VA SMG+K
Sbjct: 305 EGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSRREPSEIIANIEAVANSMGFK 364

BLAST of Lsi08G010430 vs. TAIR 10
Match: AT2G26980.3 (CBL-interacting protein kinase 3 )

HSP 1 Score: 359.8 bits (922), Expect = 2.9e-99
Identity = 191/370 (51.62%), Postives = 240/370 (64.86%), Query Frame = 0

Query: 2   VVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ-------- 61
           V R+VGKYEVGRTIGEGTFAKVKFA+N+ETGE  A+K+LD+  ++KHKM +Q        
Sbjct: 7   VKRRVGKYEVGRTIGEGTFAKVKFARNSETGEPVALKILDKEKVLKHKMAEQIRREIATM 66

Query: 62  -------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVD 121
                        V+AS+TKI+IILEY+TGGELFDKIV+ GR+ E E+R+YFQQLI  VD
Sbjct: 67  KLIKHPNVVQLYEVMASKTKIFIILEYVTGGELFDKIVNDGRMKEDEARRYFQQLIHAVD 126

Query: 122 YCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQ--GVSLLRTTCGTPNYVAPEVL 181
           YCHS+GVYHRDLKPENLLLDS GNL+ISDFGLSAL +Q     LL T+CGTPNYVAPEVL
Sbjct: 127 YCHSRGVYHRDLKPENLLLDSYGNLKISDFGLSALSQQVRDDGLLHTSCGTPNYVAPEVL 186

Query: 182 NHKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALI 241
           N +GYDGA AD+WSCG                        I   +F+CP W  +GA  LI
Sbjct: 187 NDRGYDGATADMWSCGVVLYVLLAGYLPFDDSNLMNLYKKISSGEFNCPPWLSLGAMKLI 246

Query: 242 HRIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRG 301
            RI DPNP T        +DEWFKK+Y      E +D N+DD++AVF D EE    E+R 
Sbjct: 247 TRILDPNPMTRVTPQEVFEDEWFKKDYKPPVFEERDDSNMDDIDAVFKDSEEHLVTEKR- 306

Query: 302 NEDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSM 318
             +  P A+NAF++I +S+GLNL  +FD  Q+  K  TR   +  A  ++  +E  A+ +
Sbjct: 307 --EEQPAAINAFEIISMSRGLNLENLFDPEQE-FKRETRITLRGGANEIIEKIEEAAKPL 366

BLAST of Lsi08G010430 vs. TAIR 10
Match: AT2G26980.1 (CBL-interacting protein kinase 3 )

HSP 1 Score: 359.8 bits (922), Expect = 2.9e-99
Identity = 191/370 (51.62%), Postives = 240/370 (64.86%), Query Frame = 0

Query: 2   VVRKVGKYEVGRTIGEGTFAKVKFAQNTETGESFAMKVLDRSTIIKHKMVDQ-------- 61
           V R+VGKYEVGRTIGEGTFAKVKFA+N+ETGE  A+K+LD+  ++KHKM +Q        
Sbjct: 7   VKRRVGKYEVGRTIGEGTFAKVKFARNSETGEPVALKILDKEKVLKHKMAEQIRREIATM 66

Query: 62  -------------VLASRTKIYIILEYITGGELFDKIVHHGRLSEAESRKYFQQLIDGVD 121
                        V+AS+TKI+IILEY+TGGELFDKIV+ GR+ E E+R+YFQQLI  VD
Sbjct: 67  KLIKHPNVVQLYEVMASKTKIFIILEYVTGGELFDKIVNDGRMKEDEARRYFQQLIHAVD 126

Query: 122 YCHSKGVYHRDLKPENLLLDSLGNLRISDFGLSALPEQ--GVSLLRTTCGTPNYVAPEVL 181
           YCHS+GVYHRDLKPENLLLDS GNL+ISDFGLSAL +Q     LL T+CGTPNYVAPEVL
Sbjct: 127 YCHSRGVYHRDLKPENLLLDSYGNLKISDFGLSALSQQVRDDGLLHTSCGTPNYVAPEVL 186

Query: 182 NHKGYDGAMADVWSCG------------------------IERADFSCPSWFPVGAKALI 241
           N +GYDGA AD+WSCG                        I   +F+CP W  +GA  LI
Sbjct: 187 NDRGYDGATADMWSCGVVLYVLLAGYLPFDDSNLMNLYKKISSGEFNCPPWLSLGAMKLI 246

Query: 242 HRIFDPNPST-------VKDEWFKKNYVSANLFEDEDVNLDDVNAVFDDPEEQRTEEQRG 301
            RI DPNP T        +DEWFKK+Y      E +D N+DD++AVF D EE    E+R 
Sbjct: 247 TRILDPNPMTRVTPQEVFEDEWFKKDYKPPVFEERDDSNMDDIDAVFKDSEEHLVTEKR- 306

Query: 302 NEDMSPLALNAFDLIILSQGLNLRTMFDRGQDSMKYPTRFVCQKPAKVLLSTMEVVAQSM 318
             +  P A+NAF++I +S+GLNL  +FD  Q+  K  TR   +  A  ++  +E  A+ +
Sbjct: 307 --EEQPAAINAFEIISMSRGLNLENLFDPEQE-FKRETRITLRGGANEIIEKIEEAAKPL 366

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9STV47.6e-13768.56CBL-interacting serine/threonine-protein kinase 8 OS=Arabidopsis thaliana OX=370... [more]
Q5JLD81.7e-12864.85CBL-interacting protein kinase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q9LDI31.4e-11155.59CBL-interacting serine/threonine-protein kinase 24 OS=Arabidopsis thaliana OX=37... [more]
Q69Q472.1e-11056.01CBL-interacting protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q6ZLP58.2e-9951.90CBL-interacting protein kinase 23 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Match NameE-valueIdentityDescription
A0A6J1KP026.4e-16381.57Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1GI841.4e-16281.30Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3BCG32.1e-16181.57Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0LPN84.6e-16181.30Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_2... [more]
A0A6J1BTR13.1e-15778.86Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
XP_038885498.14.4e-16683.20CBL-interacting serine/threonine-protein kinase 8 [Benincasa hispida][more]
XP_023538368.17.7e-16381.57CBL-interacting serine/threonine-protein kinase 8 [Cucurbita pepo subsp. pepo][more]
XP_023001929.11.3e-16281.57CBL-interacting serine/threonine-protein kinase 8 [Cucurbita maxima][more]
KAG6585315.12.9e-16281.30CBL-interacting serine/threonine-protein kinase 8, partial [Cucurbita argyrosper... [more]
XP_022951279.12.9e-16281.30CBL-interacting serine/threonine-protein kinase 8 [Cucurbita moschata] >KAG70202... [more]
Match NameE-valueIdentityDescription
AT4G24400.23.7e-13962.91CBL-interacting protein kinase 8 [more]
AT4G24400.15.4e-13868.56CBL-interacting protein kinase 8 [more]
AT5G35410.11.0e-11255.59Protein kinase superfamily protein [more]
AT2G26980.32.9e-9951.62CBL-interacting protein kinase 3 [more]
AT2G26980.12.9e-9951.62CBL-interacting protein kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 9..210
e-value: 1.5E-52
score: 190.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 9..175
e-value: 7.7E-44
score: 150.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 9..255
score: 31.679253
IPR004041NAF domainPFAMPF03822NAFcoord: 253..311
e-value: 2.6E-15
score: 56.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 3..257
e-value: 2.6E-62
score: 212.8
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 258..322
e-value: 3.3E-15
score: 58.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 368..382
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 368..397
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 175..317
coord: 2..175
NoneNo IPR availablePANTHERPTHR43895:SF114NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 2..175
NoneNo IPR availablePANTHERPTHR43895:SF114NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 175..317
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 15..47
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 107..119
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 251..275
score: 11.051527
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 6..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G010430.1Lsi08G010430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity