Lsi08G010340 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G010340
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mitochondrial uncoupling protein 5
Location	chr08: 18931342 .. 18932689 (+)
RNA-Seq Expression	Lsi08G010340
Synteny	Lsi08G010340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGAGAAGGTCAAAGTCAAAGAAAGTCAAGAAGTAATGGAGAGGCAGTCGCATTAGGGCATTCTGTTCTTCCTTAGACCCACCGCCGTCGCCCCTTCTTCTTCAACGCGTAAATGTAAGCTTCCGCGACTCCACCTTCTTTCTCTTTATATAAATTAATCTTCTTTACTTCACCCATTACCATTACTCCGCTTCTCATTTCTCCTCATCACTTCCTTCTTCTTTCTCAATCTCAATTTCTCTCCAGAAATGGGTTTCAAGGGATTTGCCGAAGGAGGAATCGCTTCCATTGTTGCCGGATGCTCCACTCACCCACTTGATCTCATCAAGGTTCGGATGCAATTGGACGGCGAGAAACCGCCGCTTCCAAATCTCCGCCCCGCCCTAGCTTTCAATGCTTCTCGTTCCCTTGTCGCGCCGGAATCATTCCACATTCCGCCGCCCCAACCTCCGCGTGTCGGACCCATCACGGTCGGCGTGCGTATTGTCCAGACCGAAGGTGTTGCCGCGCTGTTTTCCGGCGTTTCTGCCACTGTTCTCCGGCAGACTCTTTACTCTACCACTCGGATGGGTCTTTACGACGTCCTCAAGACGAAATGGTCCGACCCAGATTCCGGAAGTATGCCTCTAACCCGGAAAATCACGGCGGGGTTAATAGCCGGCGGGATTGGCGCGGCTGTCGGGAACCCGGCCGACGTCGCAATGGTGAGAATGCAGGCTGACGGACGGCTTCCGGTTGCTCAGCGGCGGAATTACGCCGGCGTGGTGGACGCCATCACAAGAATGTCGAAGCAGGAGGGGATTACTAGCCTGTGGCGCGGGTCGTCGCTTACGGTGAACCGGGCGATGATCGTGACGGCGGCGCAATTGGCCTCATATGATCAGATAAAAGAGACGATATTGGAAAAGGGTGTGATGAAAGACGGACTAGGGACCCACGTGACGGCGAGTTTCGCGGCGGGGTTTGTGGCGGCGGTGGCGTCGAATCCGGTGGATGTGATAAAGACGAGAGTTATGAATATGAAGGTGGAGGCCGGGGCCGTGCCACCGTACAATGGGGCGTTGGACTGTGCAATGAAGACGGTGAAGGCGGAAGGGCCGATGGCTCTTTACAAAGGATTTATTCCGACGATTTCACGGCAGGGGCCTTTCACGGTGGTCTTGTTTGTCACACTTGAACAAGTCAGGAAGATCTTCAACCAGTTTTGATTTGGATGACCGGAATTGAGTATTTTATATTATAAAAATTGTTCCCAGATTCTTTTCTATTCAATTTTAATAAAATATAATATTTACATTTCAGAAACACTTTTACATTTAAGGCGAAATTAAATAACCACGTTCAGCT

mRNA sequence

AAAGAGAAGGTCAAAGTCAAAGAAAGTCAAGAAGTAATGGAGAGGCAGTCGCATTAGGGCATTCTGTTCTTCCTTAGACCCACCGCCGTCGCCCCTTCTTCTTCAACGCGTAAATATGGGTTTCAAGGGATTTGCCGAAGGAGGAATCGCTTCCATTGTTGCCGGATGCTCCACTCACCCACTTGATCTCATCAAGGTTCGGATGCAATTGGACGGCGAGAAACCGCCGCTTCCAAATCTCCGCCCCGCCCTAGCTTTCAATGCTTCTCGTTCCCTTGTCGCGCCGGAATCATTCCACATTCCGCCGCCCCAACCTCCGCGTGTCGGACCCATCACGGTCGGCGTGCGTATTGTCCAGACCGAAGGTGTTGCCGCGCTGTTTTCCGGCGTTTCTGCCACTGTTCTCCGGCAGACTCTTTACTCTACCACTCGGATGGGTCTTTACGACGTCCTCAAGACGAAATGGTCCGACCCAGATTCCGGAAGTATGCCTCTAACCCGGAAAATCACGGCGGGGTTAATAGCCGGCGGGATTGGCGCGGCTGTCGGGAACCCGGCCGACGTCGCAATGGTGAGAATGCAGGCTGACGGACGGCTTCCGGTTGCTCAGCGGCGGAATTACGCCGGCGTGGTGGACGCCATCACAAGAATGTCGAAGCAGGAGGGGATTACTAGCCTGTGGCGCGGGTCGTCGCTTACGGTGAACCGGGCGATGATCGTGACGGCGGCGCAATTGGCCTCATATGATCAGATAAAAGAGACGATATTGGAAAAGGGTGTGATGAAAGACGGACTAGGGACCCACGTGACGGCGAGTTTCGCGGCGGGGTTTGTGGCGGCGGTGGCGTCGAATCCGGTGGATGTGATAAAGACGAGAGTTATGAATATGAAGGTGGAGGCCGGGGCCGTGCCACCGTACAATGGGGCGTTGGACTGTGCAATGAAGACGGTGAAGGCGGAAGGGCCGATGGCTCTTTACAAAGGATTTATTCCGACGATTTCACGGCAGGGGCCTTTCACGGTGGTCTTGTTTGTCACACTTGAACAAGTCAGGAAGATCTTCAACCAGTTTTGATTTGGATGACCGGAATTGAGTATTTTATATTATAAAAATTGTTCCCAGATTCTTTTCTATTCAATTTTAATAAAATATAATATTTACATTTCAGAAACACTTTTACATTTAAGGCGAAATTAAATAACCACGTTCAGCT

Coding sequence (CDS)

ATGGGTTTCAAGGGATTTGCCGAAGGAGGAATCGCTTCCATTGTTGCCGGATGCTCCACTCACCCACTTGATCTCATCAAGGTTCGGATGCAATTGGACGGCGAGAAACCGCCGCTTCCAAATCTCCGCCCCGCCCTAGCTTTCAATGCTTCTCGTTCCCTTGTCGCGCCGGAATCATTCCACATTCCGCCGCCCCAACCTCCGCGTGTCGGACCCATCACGGTCGGCGTGCGTATTGTCCAGACCGAAGGTGTTGCCGCGCTGTTTTCCGGCGTTTCTGCCACTGTTCTCCGGCAGACTCTTTACTCTACCACTCGGATGGGTCTTTACGACGTCCTCAAGACGAAATGGTCCGACCCAGATTCCGGAAGTATGCCTCTAACCCGGAAAATCACGGCGGGGTTAATAGCCGGCGGGATTGGCGCGGCTGTCGGGAACCCGGCCGACGTCGCAATGGTGAGAATGCAGGCTGACGGACGGCTTCCGGTTGCTCAGCGGCGGAATTACGCCGGCGTGGTGGACGCCATCACAAGAATGTCGAAGCAGGAGGGGATTACTAGCCTGTGGCGCGGGTCGTCGCTTACGGTGAACCGGGCGATGATCGTGACGGCGGCGCAATTGGCCTCATATGATCAGATAAAAGAGACGATATTGGAAAAGGGTGTGATGAAAGACGGACTAGGGACCCACGTGACGGCGAGTTTCGCGGCGGGGTTTGTGGCGGCGGTGGCGTCGAATCCGGTGGATGTGATAAAGACGAGAGTTATGAATATGAAGGTGGAGGCCGGGGCCGTGCCACCGTACAATGGGGCGTTGGACTGTGCAATGAAGACGGTGAAGGCGGAAGGGCCGATGGCTCTTTACAAAGGATTTATTCCGACGATTTCACGGCAGGGGCCTTTCACGGTGGTCTTGTTTGTCACACTTGAACAAGTCAGGAAGATCTTCAACCAGTTTTGA

Protein sequence

MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVRKIFNQF

Homology

[Close]

BLAST of Lsi08G010340 vs. ExPASy Swiss-Prot
Match: Q9SJY5 (Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 7.2e-130
Identity = 238/320 (74.38%), Postives = 274/320 (85.62%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPL-PNLRPALAFNASRSLVAPES 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQL GE  P+  NLRPALAF  S ++ AP  
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAP-- 60

Query: 61  FHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSD 120
                  P RVG I VG R+++ EG+ ALFSGVSATVLRQTLYSTTRMGLYD++K +W+D
Sbjct: 61  -------PLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTD 120

Query: 121 PDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRM 180
           P++ +MPL +KI AG IAG IGAAVGNPADVAMVRMQADGRLP+  RRNY  V+DAIT+M
Sbjct: 121 PETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQM 180

Query: 181 SKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGF 240
            + EG+TSLWRGSSLT+NRAM+VT++QLASYD +KETILEKG++KDGLGTHV+ASFAAGF
Sbjct: 181 IRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGF 240

Query: 241 VAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQG 300
           VA+VASNPVDVIKTRVMNMKV AG  PPY GA+DCA+KTVKAEG M+LYKGFIPT+SRQ 
Sbjct: 241 VASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQA 300

Query: 301 PFTVVLFVTLEQVRKIFNQF 320
           PFTVVLFVTLEQV+K+F  +
Sbjct: 301 PFTVVLFVTLEQVKKLFKDY 311

[Close]

BLAST of Lsi08G010340 vs. ExPASy Swiss-Prot
Match: Q9SB52 (Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.7e-126
Identity = 240/322 (74.53%), Postives = 268/322 (83.23%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPN---LRPALAFNASRSLVAP 60
           MG K F EGGIAS++AGCSTHPLDLIKVR+QL GE P       LRPALAF  S    +P
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNS----SP 60

Query: 61  ESFHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKW 120
            +F       P+VGPI++G+ IV++EG AALFSGVSAT+LRQTLYSTTRMGLY+VLK KW
Sbjct: 61  AAFLETTSSVPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKW 120

Query: 121 SDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAIT 180
           +DP+SG + L+RKI AGL+AGGIGAAVGNPADVAMVRMQADGRLP+AQRRNYAGV DAI 
Sbjct: 121 TDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIR 180

Query: 181 RMSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAA 240
            M K EG+TSLWRGS+LT+NRAMIVTAAQLASYDQ KE ILE GVM DGLGTHV ASFAA
Sbjct: 181 SMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAA 240

Query: 241 GFVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISR 300
           GFVA+VASNPVDVIKTRVMNMKV A     Y+GA DCA+KTVKAEG MALYKGF+PT+ R
Sbjct: 241 GFVASVASNPVDVIKTRVMNMKVGA-----YDGAWDCAVKTVKAEGAMALYKGFVPTVCR 300

Query: 301 QGPFTVVLFVTLEQVRKIFNQF 320
           QGPFTVVLFVTLEQVRK+   F
Sbjct: 301 QGPFTVVLFVTLEQVRKLLRDF 313

[Close]

BLAST of Lsi08G010340 vs. ExPASy Swiss-Prot
Match: Q9FY68 (Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 2.7e-100
Identity = 201/334 (60.18%), Postives = 240/334 (71.86%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGE------KPPLPNL-----------R 60
           MGFK F EGGIA+I+AG  THPLDLIKVRMQL GE      + P PNL           R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFNASRSLVAPESFHIPPPQPPR---VGPITVGVRIVQTEGVAALFSGVSATVLRQT 120
           P  A ++    ++    HI  P       + P  VG  IV+TEG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDVLKTKWSDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK +W+D  +G+ PL  KITAGLIAG +G+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPVAQRRNYAGVVDAITRMSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEK 240
           LP+ +RRNY  VVDAI R+++QEG++SLWRGS LTVNRAMIVTA+QLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 G-VMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 KAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVR 314
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVR 329

[Close]

BLAST of Lsi08G010340 vs. ExPASy Swiss-Prot
Match: Q54PY7 (Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium discoideum OX=44689 GN=ucpC PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 8.1e-65
Identity = 139/315 (44.13%), Postives = 189/315 (60.00%), Query Frame = 0


Query: 4   KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIP 63
           K F  GG+A +++   THP+D +KVRMQL GE   +                        
Sbjct: 26  KQFVIGGLAGMLSSAFTHPIDSLKVRMQLQGEGTGV------------------------ 85

Query: 64  PPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDPDSG 123
               P+ G + + V I QTEG   L+ G+SA++LRQ  Y+TTR GLYD++K   +  D  
Sbjct: 86  ---GPKRGALKMLVHINQTEGFFTLYKGLSASLLRQATYTTTRFGLYDLIKDIVAKDDK- 145

Query: 124 SMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQE 183
            +P T+KI  G+++G  GA VG PAD+ MVRMQADG+LP   RRNY  V D I R+SK+E
Sbjct: 146 PLPFTQKIMVGMLSGAGGAIVGTPADLTMVRMQADGKLPFNLRRNYKNVFDGIFRISKEE 205

Query: 184 GITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAAV 243
           GI SLW+G S  + RAM +TA Q++SYDQ K+ +L  G   D + TH+ AS  A FVAAV
Sbjct: 206 GIISLWKGCSPNLIRAMFMTAGQVSSYDQTKQLMLASGYFHDDIKTHLIASTTAAFVAAV 265

Query: 244 ASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGPFTV 303
           A++P+DVIKTR+MN          Y G  DC  KT++AEG  A YKGF P   R GP T+
Sbjct: 266 ATSPLDVIKTRIMNSPKTVTGELQYKGTFDCLSKTLRAEGFKAFYKGFNPYFMRLGPQTI 312

Query: 304 VLFVTLEQVRKIFNQ 319
           + F+ +EQ+  ++ +
Sbjct: 326 LTFIFVEQLNILWKK 312

[Close]

BLAST of Lsi08G010340 vs. ExPASy Swiss-Prot
Match: P90992 (Mitochondrial 2-oxoglutarate/malate carrier protein OS=Caenorhabditis elegans OX=6239 GN=misc-1 PE=1 SV=2)

HSP 1 Score: 230.7 bits (587), Expect = 2.3e-59
Identity = 133/314 (42.36%), Postives = 183/314 (58.28%), Query Frame = 0


Query: 6   FAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIPPP 65
           FA GG A + A     PLDL+K RMQL G                +       S H    
Sbjct: 13  FAFGGTAGMGATLVVQPLDLVKNRMQLSG---------------TTGKKEYRSSMH---- 72

Query: 66  QPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDPDSGSM 125
                        I++ EGV A+++G+SA +LRQ  Y+TTR+G Y  L  ++++ D   +
Sbjct: 73  ---------ALTSIMKNEGVFAVYNGLSAGLLRQATYTTTRLGTYAFLLERFTEKDK-PL 132

Query: 126 PLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQEGI 185
               K   G+ AGGIG+ VG PA++A++RM  DGRLPV QRRNY GVV+A+TR++K+EG+
Sbjct: 133 SFGMKAVLGMTAGGIGSFVGTPAEIALIRMTGDGRLPVEQRRNYTGVVNALTRITKEEGV 192

Query: 186 TSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAAVAS 245
            +LWRG + TV RAM+V AAQLA+Y Q K+ +L  G ++DG+  H  AS  +G    +AS
Sbjct: 193 LTLWRGCTPTVLRAMVVNAAQLATYSQAKQALLASGKVQDGIFCHFLASMISGLATTIAS 252

Query: 246 NPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGPFTVVL 305
            PVD+ KTR+ +MKV  G  P Y  A D   K +K EG  AL+KGF P   R GP TV+ 
Sbjct: 253 MPVDIAKTRIQSMKVIDGK-PEYKNAFDVWGKVIKNEGIFALWKGFTPYYMRLGPHTVLT 296

Query: 306 FVTLEQVRKIFNQF 320
           F+ LEQ+   + Q+
Sbjct: 313 FIILEQMNAAYFQY 296

[Close]

BLAST of Lsi08G010340 vs. ExPASy TrEMBL
Match: A0A5A7V9T0 (Mitochondrial uncoupling protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004130 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 4.8e-169
Identity = 309/319 (96.87%), Postives = 315/319 (98.75%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGF EGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS+VA ESF
Sbjct: 1   MGFKGFVEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSVVAQESF 60

Query: 61  HIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDP 120
           HIPPPQPPRVGPI+VGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYD+LKTKWSDP
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGS+LTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAGA  PY+GALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGATAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

[Close]

BLAST of Lsi08G010340 vs. ExPASy TrEMBL
Match: A0A0A0LLU3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371610 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.4e-168
Identity = 308/318 (96.86%), Postives = 314/318 (98.74%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60

Query: 61  HIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDP 120
           HIPPPQPPRVGPI+VGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYD+LKTKWS+P
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSNP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGS+LTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG   PY+GALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGEAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQ 319
           FTVVLFVTLEQVRKIFNQ
Sbjct: 301 FTVVLFVTLEQVRKIFNQ 318

[Close]

BLAST of Lsi08G010340 vs. ExPASy TrEMBL
Match: A0A6J1GH78 (mitochondrial uncoupling protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC111454164 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 5.5e-165
Identity = 302/321 (94.08%), Postives = 313/321 (97.51%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS--LVAPE 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLP+LRPA+AFN SRS  +V PE
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPSLRPAVAFNVSRSAAVVTPE 60

Query: 61  SFHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS 120
           SFHIPPPQPPRVGPI+VGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYD+LKTKWS
Sbjct: 61  SFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWS 120

Query: 121 DPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180
           DP+SG+MPL+RKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR
Sbjct: 121 DPNSGNMPLSRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180

Query: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAG 240
           MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASF AG
Sbjct: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFTAG 240

Query: 241 FVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQ 300
           FVAAVASNPVDVIKTRVMNMKVEAGA PPY+GALDCA+KTVKAEGPMALYKGFIPTISRQ
Sbjct: 241 FVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVKAEGPMALYKGFIPTISRQ 300

Query: 301 GPFTVVLFVTLEQVRKIFNQF 320
           GPFTVVLFVTLEQVRK FNQF
Sbjct: 301 GPFTVVLFVTLEQVRKFFNQF 321

[Close]

BLAST of Lsi08G010340 vs. ExPASy TrEMBL
Match: A0A6J1KQ28 (mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111496577 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 9.4e-165
Identity = 304/321 (94.70%), Postives = 313/321 (97.51%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS--LVAPE 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLP+LRPA+AFNASRS  +V PE
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPSLRPAVAFNASRSAAVVTPE 60

Query: 61  SFHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS 120
            FHIPPPQPPRVGPI+VGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS
Sbjct: 61  YFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS 120

Query: 121 DPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180
           DP+SG+MPL+RKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR
Sbjct: 121 DPNSGNMPLSRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180

Query: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAG 240
           MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASF AG
Sbjct: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFTAG 240

Query: 241 FVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQ 300
           FVAAVASNPVDVIKTRVMNMKVEAGA  PY+GALDCAMKTVKAEGPMALYKGFIPTISRQ
Sbjct: 241 FVAAVASNPVDVIKTRVMNMKVEAGAAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQ 300

Query: 301 GPFTVVLFVTLEQVRKIFNQF 320
           GPFTVVLFVTLEQVRKIFNQF
Sbjct: 301 GPFTVVLFVTLEQVRKIFNQF 321

[Close]

BLAST of Lsi08G010340 vs. ExPASy TrEMBL
Match: A0A0A0LPN1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371110 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 9.4e-165
Identity = 301/319 (94.36%), Postives = 312/319 (97.81%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQL GEKP LPNL PALAFNASRS+VAP+ +
Sbjct: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLAGEKPALPNLPPALAFNASRSVVAPDYY 60

Query: 61  HIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDP 120
           HIPPPQPPRVGPI+VG+RIVQ+EGV+ALFSGVSATVLRQTLYSTTRMGLYD+LKT+WSDP
Sbjct: 61  HIPPPQPPRVGPISVGMRIVQSEGVSALFSGVSATVLRQTLYSTTRMGLYDILKTRWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGS+LTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG   PY+GALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGEAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

[Close]

BLAST of Lsi08G010340 vs. NCBI nr
Match: XP_038884031.1 (mitochondrial uncoupling protein 5-like [Benincasa hispida])

HSP 1 Score: 605.1 bits (1559), Expect = 3.4e-169
Identity = 308/319 (96.55%), Postives = 316/319 (99.06%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGF EGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS+VAPESF
Sbjct: 1   MGFKGFVEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSVVAPESF 60

Query: 61  HIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDP 120
           HIPPPQPPRVGPI+VGVRIVQ+EGV ALFSGVSATVLRQTLYSTTRMGLYD+LKTKWSDP
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVTALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           +SGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLP+AQRRNYAGVVDAITRMS
Sbjct: 121 NSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPMAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQ+KETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQMKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAGA PPY+GALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

[Close]

BLAST of Lsi08G010340 vs. NCBI nr
Match: KAA0065082.1 (mitochondrial uncoupling protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 603.6 bits (1555), Expect = 9.9e-169
Identity = 309/319 (96.87%), Postives = 315/319 (98.75%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MGFKGF EGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS+VA ESF
Sbjct: 1   MGFKGFVEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSVVAQESF 60

Query: 61  HIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDP 120
           HIPPPQPPRVGPI+VGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYD+LKTKWSDP
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSDP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGS+LTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAGA  PY+GALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGATAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQF 320
           FTVVLFVTLEQVRKIFNQF
Sbjct: 301 FTVVLFVTLEQVRKIFNQF 319

[Close]

BLAST of Lsi08G010340 vs. NCBI nr
Match: XP_031736495.1 (mitochondrial uncoupling protein 5 [Cucumis sativus] >XP_031736496.1 mitochondrial uncoupling protein 5 [Cucumis sativus] >KAE8652145.1 hypothetical protein Csa_022491 [Cucumis sativus])

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 307/318 (96.54%), Postives = 314/318 (98.74%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESF 60

Query: 61  HIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDP 120
           HIPPPQPPRVGPI+VGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYD+LKTKWS+P
Sbjct: 61  HIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWSNP 120

Query: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 180
           DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPV+QRRNYAGVVDAITRMS
Sbjct: 121 DSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVSQRRNYAGVVDAITRMS 180

Query: 181 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240
           KQEGITSLWRGS+LTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV
Sbjct: 181 KQEGITSLWRGSALTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 240

Query: 241 AAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300
           AAVASNPVDVIKTRVMNMKVEAG   PY+GALDCAMKTVKAEGPMALYKGFIPTISRQGP
Sbjct: 241 AAVASNPVDVIKTRVMNMKVEAGEAAPYSGALDCAMKTVKAEGPMALYKGFIPTISRQGP 300

Query: 301 FTVVLFVTLEQVRKIFNQ 319
           FTVVLFVTLEQVRKIFNQ
Sbjct: 301 FTVVLFVTLEQVRKIFNQ 318

[Close]

BLAST of Lsi08G010340 vs. NCBI nr
Match: XP_023538024.1 (mitochondrial uncoupling protein 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592.8 bits (1527), Expect = 1.7e-165
Identity = 304/321 (94.70%), Postives = 314/321 (97.82%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS--LVAPE 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLP+LRPA+AFN SRS  +V PE
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPSLRPAVAFNVSRSAAVVTPE 60

Query: 61  SFHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS 120
           SFHIPPPQPPRVGPI+VGVRIVQ+EG+AALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS
Sbjct: 61  SFHIPPPQPPRVGPISVGVRIVQSEGIAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS 120

Query: 121 DPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180
           DP+SG+MPL+RKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR
Sbjct: 121 DPNSGNMPLSRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180

Query: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAG 240
           MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASF AG
Sbjct: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFTAG 240

Query: 241 FVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQ 300
           FVAAVASNPVDVIKTRVMNMKVEAGA PPY+GALDCAMKTVKAEGPMALYKGFIPTISRQ
Sbjct: 241 FVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCAMKTVKAEGPMALYKGFIPTISRQ 300

Query: 301 GPFTVVLFVTLEQVRKIFNQF 320
           GPFTVVLFVTLEQVRKIFNQF
Sbjct: 301 GPFTVVLFVTLEQVRKIFNQF 321

[Close]

BLAST of Lsi08G010340 vs. NCBI nr
Match: XP_022951282.1 (mitochondrial uncoupling protein 5-like [Cucurbita moschata])

HSP 1 Score: 590.1 bits (1520), Expect = 1.1e-164
Identity = 302/321 (94.08%), Postives = 313/321 (97.51%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRS--LVAPE 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLP+LRPA+AFN SRS  +V PE
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPSLRPAVAFNVSRSAAVVTPE 60

Query: 61  SFHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWS 120
           SFHIPPPQPPRVGPI+VGVRIVQ+EGVAALFSGVSATVLRQTLYSTTRMGLYD+LKTKWS
Sbjct: 61  SFHIPPPQPPRVGPISVGVRIVQSEGVAALFSGVSATVLRQTLYSTTRMGLYDILKTKWS 120

Query: 121 DPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180
           DP+SG+MPL+RKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR
Sbjct: 121 DPNSGNMPLSRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITR 180

Query: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAG 240
           MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASF AG
Sbjct: 181 MSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFTAG 240

Query: 241 FVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQ 300
           FVAAVASNPVDVIKTRVMNMKVEAGA PPY+GALDCA+KTVKAEGPMALYKGFIPTISRQ
Sbjct: 241 FVAAVASNPVDVIKTRVMNMKVEAGAAPPYSGALDCALKTVKAEGPMALYKGFIPTISRQ 300

Query: 301 GPFTVVLFVTLEQVRKIFNQF 320
           GPFTVVLFVTLEQVRK FNQF
Sbjct: 301 GPFTVVLFVTLEQVRKFFNQF 321

[Close]

BLAST of Lsi08G010340 vs. TAIR 10
Match: AT2G22500.1 (uncoupling protein 5 )

HSP 1 Score: 464.9 bits (1195), Expect = 5.1e-131
Identity = 238/320 (74.38%), Postives = 274/320 (85.62%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPL-PNLRPALAFNASRSLVAPES 60
           MG KGFAEGGIASIVAGCSTHPLDLIKVRMQL GE  P+  NLRPALAF  S ++ AP  
Sbjct: 1   MGLKGFAEGGIASIVAGCSTHPLDLIKVRMQLQGESAPIQTNLRPALAFQTSTTVNAP-- 60

Query: 61  FHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSD 120
                  P RVG I VG R+++ EG+ ALFSGVSATVLRQTLYSTTRMGLYD++K +W+D
Sbjct: 61  -------PLRVGVIGVGSRLIREEGMRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTD 120

Query: 121 PDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRM 180
           P++ +MPL +KI AG IAG IGAAVGNPADVAMVRMQADGRLP+  RRNY  V+DAIT+M
Sbjct: 121 PETKTMPLMKKIGAGAIAGAIGAAVGNPADVAMVRMQADGRLPLTDRRNYKSVLDAITQM 180

Query: 181 SKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGF 240
            + EG+TSLWRGSSLT+NRAM+VT++QLASYD +KETILEKG++KDGLGTHV+ASFAAGF
Sbjct: 181 IRGEGVTSLWRGSSLTINRAMLVTSSQLASYDSVKETILEKGLLKDGLGTHVSASFAAGF 240

Query: 241 VAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQG 300
           VA+VASNPVDVIKTRVMNMKV AG  PPY GA+DCA+KTVKAEG M+LYKGFIPT+SRQ 
Sbjct: 241 VASVASNPVDVIKTRVMNMKVVAGVAPPYKGAVDCALKTVKAEGIMSLYKGFIPTVSRQA 300

Query: 301 PFTVVLFVTLEQVRKIFNQF 320
           PFTVVLFVTLEQV+K+F  +
Sbjct: 301 PFTVVLFVTLEQVKKLFKDY 311

[Close]

BLAST of Lsi08G010340 vs. TAIR 10
Match: AT4G24570.1 (dicarboxylate carrier 2 )

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-127
Identity = 240/322 (74.53%), Postives = 268/322 (83.23%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPN---LRPALAFNASRSLVAP 60
           MG K F EGGIAS++AGCSTHPLDLIKVR+QL GE P       LRPALAF  S    +P
Sbjct: 1   MGVKSFVEGGIASVIAGCSTHPLDLIKVRLQLHGEAPSTTTVTLLRPALAFPNS----SP 60

Query: 61  ESFHIPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKW 120
            +F       P+VGPI++G+ IV++EG AALFSGVSAT+LRQTLYSTTRMGLY+VLK KW
Sbjct: 61  AAFLETTSSVPKVGPISLGINIVKSEGAAALFSGVSATLLRQTLYSTTRMGLYEVLKNKW 120

Query: 121 SDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAIT 180
           +DP+SG + L+RKI AGL+AGGIGAAVGNPADVAMVRMQADGRLP+AQRRNYAGV DAI 
Sbjct: 121 TDPESGKLNLSRKIGAGLVAGGIGAAVGNPADVAMVRMQADGRLPLAQRRNYAGVGDAIR 180

Query: 181 RMSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAA 240
            M K EG+TSLWRGS+LT+NRAMIVTAAQLASYDQ KE ILE GVM DGLGTHV ASFAA
Sbjct: 181 SMVKGEGVTSLWRGSALTINRAMIVTAAQLASYDQFKEGILENGVMNDGLGTHVVASFAA 240

Query: 241 GFVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISR 300
           GFVA+VASNPVDVIKTRVMNMKV A     Y+GA DCA+KTVKAEG MALYKGF+PT+ R
Sbjct: 241 GFVASVASNPVDVIKTRVMNMKVGA-----YDGAWDCAVKTVKAEGAMALYKGFVPTVCR 300

Query: 301 QGPFTVVLFVTLEQVRKIFNQF 320
           QGPFTVVLFVTLEQVRK+   F
Sbjct: 301 QGPFTVVLFVTLEQVRKLLRDF 313

[Close]

BLAST of Lsi08G010340 vs. TAIR 10
Match: AT5G09470.1 (dicarboxylate carrier 3 )

HSP 1 Score: 366.7 bits (940), Expect = 1.9e-101
Identity = 201/334 (60.18%), Postives = 240/334 (71.86%), Query Frame = 0


Query: 1   MGFKGFAEGGIASIVAGCSTHPLDLIKVRMQLDGE------KPPLPNL-----------R 60
           MGFK F EGGIA+I+AG  THPLDLIKVRMQL GE      + P PNL           R
Sbjct: 1   MGFKPFLEGGIAAIIAGALTHPLDLIKVRMQLQGEHSFSLDQNPNPNLSLDHNLPVKPYR 60

Query: 61  PALAFNASRSLVAPESFHIPPPQPPR---VGPITVGVRIVQTEGVAALFSGVSATVLRQT 120
           P  A ++    ++    HI  P       + P  VG  IV+TEG AALFSGVSAT+LRQ 
Sbjct: 61  PVFALDSLIGSISLLPLHIHAPSSSTRSVMTPFAVGAHIVKTEGPAALFSGVSATILRQM 120

Query: 121 LYSTTRMGLYDVLKTKWSDPDSGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGR 180
           LYS TRMG+YD LK +W+D  +G+ PL  KITAGLIAG +G+ VGNPADVAMVRMQADG 
Sbjct: 121 LYSATRMGIYDFLKRRWTDQLTGNFPLVTKITAGLIAGAVGSVVGNPADVAMVRMQADGS 180

Query: 181 LPVAQRRNYAGVVDAITRMSKQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEK 240
           LP+ +RRNY  VVDAI R+++QEG++SLWRGS LTVNRAMIVTA+QLA+YD +KE ++  
Sbjct: 181 LPLNRRRNYKSVVDAIDRIARQEGVSSLWRGSWLTVNRAMIVTASQLATYDHVKEILVAG 240

Query: 241 G-VMKDGLGTHVTASFAAGFVAAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTV 300
           G     G+GTHV ASFAAG VAAVASNP+DV+KTR+MN   E      Y G LDCA+K V
Sbjct: 241 GRGTPGGIGTHVAASFAAGIVAAVASNPIDVVKTRMMNADKEI-----YGGPLDCAVKMV 300

Query: 301 KAEGPMALYKGFIPTISRQGPFTVVLFVTLEQVR 314
             EGPMALYKG +PT +RQGPFT++LF+TLEQVR
Sbjct: 301 AEEGPMALYKGLVPTATRQGPFTMILFLTLEQVR 329

[Close]

BLAST of Lsi08G010340 vs. TAIR 10
Match: AT5G58970.1 (uncoupling protein 2 )

HSP 1 Score: 204.5 bits (519), Expect = 1.2e-52
Identity = 124/316 (39.24%), Postives = 174/316 (55.06%), Query Frame = 0


Query: 6   FAEGGIASIVAGC----STHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFH 65
           F E  I S  A C     T PLD  KVR+QL                   R +   +  +
Sbjct: 12  FLETFICSAFAACFAELCTIPLDTAKVRLQL------------------QRKIPTGDGEN 71

Query: 66  IPPPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDPD 125
           +P  +    G I     I + EG++ L+ GV A + RQ +Y   R+GLY+ +KT     D
Sbjct: 72  LPKYR----GSIGTLATIAREEGISGLWKGVIAGLHRQCIYGGLRIGLYEPVKTLLVGSD 131

Query: 126 -SGSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMS 185
             G +PL +KI A L+ G I   V NP D+  VR+Q++G+LP    R YAG VDA   + 
Sbjct: 132 FIGDIPLYQKILAALLTGAIAIIVANPTDLVKVRLQSEGKLPAGVPRRYAGAVDAYFTIV 191

Query: 186 KQEGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFV 245
           K EG+++LW G    + R  IV AA+LASYDQIKETI++    +D + TH+ A  AAGF 
Sbjct: 192 KLEGVSALWTGLGPNIARNAIVNAAELASYDQIKETIMKIPFFRDSVLTHLLAGLAAGFF 251

Query: 246 AAVASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGP 305
           A    +P+DV+K+R+M           Y   +DC +KT+K EG MA YKGF+P  +R G 
Sbjct: 252 AVCIGSPIDVVKSRMMGDST-------YRNTVDCFIKTMKTEGIMAFYKGFLPNFTRLGT 298

Query: 306 FTVVLFVTLEQVRKIF 317
           +  ++F+TLEQV+K+F
Sbjct: 312 WNAIMFLTLEQVKKVF 298

[Close]

BLAST of Lsi08G010340 vs. TAIR 10
Match: AT3G54110.1 (plant uncoupling mitochondrial protein 1 )

HSP 1 Score: 203.8 bits (517), Expect = 2.1e-52
Identity = 124/312 (39.74%), Postives = 180/312 (57.69%), Query Frame = 0


Query: 4   KGFAEGGIASIVAGCSTHPLDLIKVRMQLDGEKPPLPNLRPALAFNASRSLVAPESFHIP 63
           K FA    A+ V    T PLD  KVR+QL          + ALA + +            
Sbjct: 13  KTFACSAFAACVGEVCTIPLDTAKVRLQLQ---------KSALAGDVTL----------- 72

Query: 64  PPQPPRVGPITVGVRIVQTEGVAALFSGVSATVLRQTLYSTTRMGLYDVLKTKWSDPD-S 123
           P     +G  TVG  I + EG+ +L+ GV   + RQ L+   R+G+Y+ +K  +   D  
Sbjct: 73  PKYRGLLG--TVGT-IAREEGLRSLWKGVVPGLHRQCLFGGLRIGMYEPVKNLYVGKDFV 132

Query: 124 GSMPLTRKITAGLIAGGIGAAVGNPADVAMVRMQADGRLPVAQRRNYAGVVDAITRMSKQ 183
           G +PL++KI AGL  G +G  V NP D+  VR+QA+G+L     R Y+G ++A + + +Q
Sbjct: 133 GDVPLSKKILAGLTTGALGIMVANPTDLVKVRLQAEGKLAAGAPRRYSGALNAYSTIVRQ 192

Query: 184 EGITSLWRGSSLTVNRAMIVTAAQLASYDQIKETILEKGVMKDGLGTHVTASFAAGFVAA 243
           EG+ +LW G    V R  I+ AA+LASYDQ+KETIL+     D + TH+ +   AGF A 
Sbjct: 193 EGVRALWTGLGPNVARNAIINAAELASYDQVKETILKIPGFTDNVVTHILSGLGAGFFAV 252

Query: 244 VASNPVDVIKTRVMNMKVEAGAVPPYNGALDCAMKTVKAEGPMALYKGFIPTISRQGPFT 303
              +PVDV+K+R+M    ++GA   Y G +DC +KT+K++GPMA YKGFIP   R G + 
Sbjct: 253 CIGSPVDVVKSRMMG---DSGA---YKGTIDCFVKTLKSDGPMAFYKGFIPNFGRLGSWN 295

Query: 304 VVLFVTLEQVRK 315
           V++F+TLEQ +K
Sbjct: 313 VIMFLTLEQAKK 295

The following BLAST results are available for this feature:

BLAST of Lsi08G010340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJY5	7.2e-130	74.38	Mitochondrial uncoupling protein 5 OS=Arabidopsis thaliana OX=3702 GN=PUMP5 PE=2...	[more]
Q9SB52	1.7e-126	74.53	Mitochondrial uncoupling protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUMP4 PE=2...	[more]
Q9FY68	2.7e-100	60.18	Mitochondrial uncoupling protein 6 OS=Arabidopsis thaliana OX=3702 GN=PUMP6 PE=2...	[more]
Q54PY7	8.1e-65	44.13	Probable mitochondrial 2-oxoglutarate/malate carrier protein OS=Dictyostelium di...	[more]
P90992	2.3e-59	42.36	Mitochondrial 2-oxoglutarate/malate carrier protein OS=Caenorhabditis elegans OX...	[more]

BLAST of Lsi08G010340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7V9T0	4.8e-169	96.87	Mitochondrial uncoupling protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A0A0LLU3	2.4e-168	96.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371610 PE=3 SV=1	[more]
A0A6J1GH78	5.5e-165	94.08	mitochondrial uncoupling protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1KQ28	9.4e-165	94.70	mitochondrial uncoupling protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111496...	[more]
A0A0A0LPN1	9.4e-165	94.36	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G371110 PE=3 SV=1	[more]

BLAST of Lsi08G010340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884031.1	3.4e-169	96.55	mitochondrial uncoupling protein 5-like [Benincasa hispida]	[more]
KAA0065082.1	9.9e-169	96.87	mitochondrial uncoupling protein 5 [Cucumis melo var. makuwa]	[more]
XP_031736495.1	1.1e-167	96.54	mitochondrial uncoupling protein 5 [Cucumis sativus] >XP_031736496.1 mitochondri...	[more]
XP_023538024.1	1.7e-165	94.70	mitochondrial uncoupling protein 5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022951282.1	1.1e-164	94.08	mitochondrial uncoupling protein 5-like [Cucurbita moschata]	[more]

BLAST of Lsi08G010340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22500.1	5.1e-131	74.38	uncoupling protein 5	[more]
AT4G24570.1	1.2e-127	74.53	dicarboxylate carrier 2	[more]
AT5G09470.1	1.9e-101	60.18	dicarboxylate carrier 3	[more]
AT5G58970.1	1.2e-52	39.24	uncoupling protein 2	[more]
AT3G54110.1	2.1e-52	39.74	plant uncoupling mitochondrial protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002067	Mitochondrial carrier protein	PRINTS	PR00926	MITOCARRIER	coord: 5..18 score: 30.68 coord: 18..32 score: 24.26 coord: 140..158 score: 25.19 coord: 234..256 score: 28.65
IPR023395	Mitochondrial carrier domain superfamily	GENE3D	1.50.40.10	Mitochondrial carrier domain	coord: 4..318 e-value: 8.4E-84 score: 282.9
IPR023395	Mitochondrial carrier domain superfamily	SUPERFAMILY	103506	Mitochondrial carrier	coord: 4..312
IPR018108	Mitochondrial substrate/solute carrier	PFAM	PF00153	Mito_carr	coord: 4..116 e-value: 6.9E-16 score: 57.9 coord: 228..318 e-value: 4.8E-19 score: 68.1 coord: 125..219 e-value: 7.4E-19 score: 67.5
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 225..315 score: 26.396021
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 125..216 score: 26.5891
IPR018108	Mitochondrial substrate/solute carrier	PROSITE	PS50920	SOLCAR	coord: 4..116 score: 17.428625
None	No IPR available	PANTHER	PTHR45618	MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED	coord: 1..319
None	No IPR available	PANTHER	PTHR45618:SF45	MITOCHONDRIAL UNCOUPLING PROTEIN 5	coord: 1..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G010340.1	Lsi08G010340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071423	malate transmembrane transport
biological_process	GO:0006839	mitochondrial transport
biological_process	GO:1902356	oxaloacetate(2-) transmembrane transport
biological_process	GO:0035435	phosphate ion transmembrane transport
biological_process	GO:0071422	succinate transmembrane transport
biological_process	GO:1902358	sulfate transmembrane transport
biological_process	GO:0015709	thiosulfate transport
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0031966	mitochondrial membrane
molecular_function	GO:0015297	antiporter activity
molecular_function	GO:0015140	malate transmembrane transporter activity
molecular_function	GO:0015131	oxaloacetate transmembrane transporter activity
molecular_function	GO:0015141	succinate transmembrane transporter activity
molecular_function	GO:0015116	sulfate transmembrane transporter activity
molecular_function	GO:0015117	thiosulfate transmembrane transporter activity