Lsi08G010080 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G010080
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein CutA
Locationchr08: 18628536 .. 18633541 (+)
RNA-Seq ExpressionLsi08G010080
SyntenyLsi08G010080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCAAAGTGAAGGCATCAAGTAAGCTCAAGACGGGGCGTCGAAAAGGCGCGACACGAAAATAGCGCTATAAAAGAGAAAATGGCTTTCACTCTCTGCAGCAGAGTTGTGACTCCATTGACATCGTCGTTTGCGTTACGACGTCGTTTACCTCTGGTTGGTGCGTTTTGCGTACTGAGCTTCGGAATCACCAATCTCTTCGTCTCCAAAACGGGTTCGGCTCTGTCTCTGCCTTTCGCTCCTCTCTTGAGGTACTTCTTTTCGCTTCCTTGCTTTCTTTCTTGATCAATTTCTTCTACGACTGTTTCTTTCTGTGAATTTGCTTTAGTTACTGGTTAATTTGTTTAGGTTATTAGTTTGATCCGTATTCGTGTTGGTTTGCTAATGGAAAACGTATGTCAGATCGAAGTTTGCTGGTCAAGGACCAGCGAGGAACGTACATTTGATAAAAATGGAAGGGAGTAGTGCTGCAGTGCCTAGCATTGTTGTTTATGTCACCGTTCCTAACAGGGAAGCAGGTTCTCTTTTATCTATCTGCTCTAGAAGTCTCGCGCTCTTTGTGGTTTTGAATTCATTGATCATTTCCTGGTATTTTGAGCATGTGAACAAACTATAATCGGATTAGAATTGATATTTTTAGTAGCTATATTTTATTTCATTTTTTGAGGTTTCCAGTATCTTGTGTACGGAAAAACTTGGTTTCTTGTTAGTTAATCTAGTGACCAATGAATCGGGCTCTTCTAGCAGCGGTATATACACTTTGTTTGATAGTAACATAGACCATATTGATCAAGTAGTATGGGAAATTGGGAATTTACGATATGGTTTCAGCATTTTACTTGTTATGCTAGTCACGTCTTTACTGTAAGGAGAGTTTTTGGAGGAAACTTATGTTGATGGTGTTGAGTGTTGATAGGATCATCTTGATTATGAGTTAGATACATATACTTGGCGCATTGACTAATAATTACCAGACTCGATAAGCAAATGATGGATTATATTATAACTCAAGAAATGCTCTAATGTTATTATATTGGATATACTATTGTTGAATATGTAGCATGTGTTCCTACTCCTAGCATACTCACTTGGATGATTAATGTTCTGAGATAGTAAGGGCCTATTTGGTATGTTATATGTTTTCAAATTCACCAAGCTAAATGGATTCAACGAAGATTTGTTTGATATATATATATATATATTTAAATTGTTTTTGGATTATGTGATCCAAATAGTGTAAATTTTAAAATCAACTTTTTTATGTTAGTGTAGTTCAAATTTAAAATTTGAAGTTGTAAAATGCAGATTTATATTAAAAAAGATAGAGGTTTAGATAAATATAGCATCTTATGTATAAACTCATATCATCCCTGTTAAAATTAAATTATATATTACATAATATATTATAAGTTATGTCTTACTGTAAAATAATATTTATACAAAATTTTGGACATAAAACATGTTTCATAAATTAATTAGTAATAATTGAGGTACGACCTAATATTTAGTTGGTAACTAAGTAGAACTATGGTTTATAAATATATTATAACACATTTTCTCCAAATTTTAAAATTGGGATCCAATTGCAAAACCTGTTACCAAATACATAATAAAAAAGATTGAAAACATGAAATACCCCCCCCCCCCCCCCAAAAAAAAAAATTGAATTTTTTGTTTTTATATCACACCAAACATGTCCTAAGCTTTTGGAAAAGTTACCCGAGAGTACAGAATTGCTACGTTTTTTGCTACCCCTACAAAAAATTTAACAAATTTGTATACATTTTTGGGGGGCATGAGGCCATTTGTTTTCGTTGTTGTTATTGTTATTATTGTTTTGTTTTGTTATTTCAGTTTCAACTTCTTTGTTTCCCTTTTAGATCTGTGCTAACAGAACTAGTCCGATCCAATGTTTTGTATTTCAATTGCATTAGGTTACCTTGTTGTTTCTGTTGTGTCCACGTACATTTATTTATTTTTCAAAATGTAAATGATATATCTAACCTTGTACTTAAGTATATAGCAAGCATTGGATTGATGTTGCCCAAGCTTAAGTATAGTGTGTTTCATAAGACTCAGCGTTGGATTCCGTACTATGGGATTTACTTCTCTTTAGTAGATGTTATGTTTCTAATCTCACTCATTCACGTTATTTCTGCAGGTAAGAAACTGGCTGAAAGCATTGTCAAAGAAAAACTTGCTGCTTGTGTTAACATAGTACCTGGTATGTGCATCTTCTCAACCTCTGCTCGTCATCTTAATGTGACAGAATGCATTGCTCACGGTTTGGAAAAGATATTATGTCAACAATATTTATAATCCATGCTTGCAGAACATCTGATATTCTCGTTTAAGTTTTCAATGCATATCAACAAAGTTGCTTCTAACTATTTAAAACCTTAAATTGAAGCTTACTGGATCTTCCCTGTAACATTTCATTGCTGTTTTTTAGTGAAGGAAAGTCGTTAGAAATAGTTTACTTGGTCATGTTGGGAAACAAAAGAAAATTTCTTTTGACCCGTTGACTCTTTAGATCTTGTGATGTCACAAACAGAAGTGATGCTTCAAGATGCTCCACATCCCTGTTTATTGTTCAATTTCAAACCACTGAATTCTTTTTCTTTTAGAGATTACTTTTCATTTTCTTATGTGAATATGAATTCTCAGACTTCTAAGTTCTAATGAATACTTTACAAATGCAGGCATAGAATCTGTTTACCAGTGGAAGGGAGAGGTATGGTTCTCATTTTCATAAATTGATATATAGTTATCACGAACAAAAAGAACTATAGGCTTTCTGTTTCTGGTTGACACAGATCCAGACAGACCCTGAGGAACTTCTGATTATTAAGACCAGGCAATCACTTCTGGGAGCACTGACAGATCATGTAAAGGCAAATCACCCATACGAGTAAGCCTAATATTCATTATTTGTTTTTGTCTGTTTTTTGTCTTCCGTTTTGTTTTGTTTCGGGTTTGTTAGTTTTTTGGGTGCATGTGTTTTTATTTTTCAATATTGGGGGAAGAATGGAGCGGAAGGGAAACTCAATAGAGTTGTGAATTGTGAACGGTTTCGCTTATGAGCTAGACATTTAGGGGGCATGTGGGGCAAGGAGTGGAGGTGTGAAGTCCCGAGTCCATAGTGTAAGGAGTTAATAAGGTATAAATTAATTGATGTTTTTTAGTAGTGGGATCCACGAACTCCTTGGGTCAAACAAGGAGTGGAGTTCACAACTCCTTACTTCCCAACTCTTTGGACCAAACGCCCCCTTAAGGTTTTTTTGGACGATATTTTTATTTTGTTAACCATATCCATTTGCTTTTTTTCCTGATTTACTCGTGTACTTTCAGGAATGTATTTNTTTATAAAAAAAATCTTAAATTAATATAGATTAATTTCAGGGGAGTTTTTAAAATTAGAAAAATAAGGGAAACTATTTACACAAAATAGCAAACTTTTTAGATAGTTGTGATAGACGCGAATAGAAGTCTATCAGGATCTATCAGCGATAGAAATAATAGAAGTCTATCACTGATAGATGCTGATATTATAATATTAAATTGATGTATAGATTAATGAATAGTCGTAAAGGCATTTTTGGAAAATTATGTAAATTAAAGGCATTTCCAAGTACAAATCTTGCGAAACAAACATAGTTATCTAAGTATTCCCAATCCCAATTATCCGTAAAAATATTCTTACAAAACAAAATGGTTATTTAAAGATGTCAGACATCTGTAATTTAGGACATTCAAAGATTTCAATTATCTAGATTCAGTTAATAGGGCAATTGCTTCACCATGCAATGCTCAGTATTAAAGGACTCACAATATTGTTTAACCCCTAGTCATTTAAACTCGTGATTTCAAAGGCATTTGGGCTTACAAGTTATCCTTTGACCATTTGACTAATTCAAGGTAGTTTCTTAGAAACTGTACATTAGTAGGTCAGAAAAGAAATAGACAACTGATTAACTTTTATATTTAACATTTTAAAATACCTTTTATCAGAATTCATTAATTCTTTATGGCACCTCAAAATGATCTTTGAATAAACTGAAATATGGACTAAACAGAAAGAACCACGTACTGCTCTTTGCCACCACATTTCTTAACTATGTTCCTGTGTCTAATGGAACCCTTAGGACAAGGATAAACCTGCAAAATATGACGGTAAGTTTTGAGCAGCTAATACATTGAGCTAATAGTTTGATGCAAGGCATTAACTGTAGACTTAATATTCAAAGCAAACAAAAAGTGATAGTTTGACACAAGTTTACTGGGATCAATATTTTTTTTTGGATAAGAAACCATTTCATTGATAAATGAAATATCCAAAGTACAACATCTACCCCCCCCCCCCCCCCCCCCCCCAAAAAAAAAAAAAAAAAAAAAGAAACCATTAAAAGCCAAATTTTGGCCTCTCCAAGGATTTACCTCCATTTTTTATGCAGGGTGCCTGAAGTTATTGCTTTGCCTATCAATGGTGGCAGCCTCGAGTACCTAGAGTGGATCAAGAGCAGCACAAAGGACTGACTTTCTCTGCGTTAACAAGTTTATTCGAGTATATCAAGTATTCTGGTTAATTTGGTATATGAACCCCATCCCCACCTTATGCTCGATGTTGTCTTGTAAATAAGAGTTGAAATTTGGTCTCTATATTGATATCTTTTGTCCTTCAATCTCCCTGCATTGGGTTTGTCAATGACTCATTCCCTAAACTTACATGGGGAAAATACATGGGGAAAAACTGCGGGAGTTTTTTTACTTAGTTCGAGGTCAGAAATCTCAACCTCAAAACTTCTCATTGCTTCACATTTGATAATGATTTCCACGAAACGATTGTTGGTCAAATGCCTTCCTGTATCGTCTTGCAAATAGCTCCATTTGGTGAAGAATTTTTATGTGAACATTTGAGACGAAAGTGTATGGATGGTATCTTCTTTTATGTGTTTTCACAAGACTTTCGTAACATCATCCCAGAATCTACCCAAAGTTTATAAGTTAG

mRNA sequence

TCCAAAGTGAAGGCATCAAGTAAGCTCAAGACGGGGCGTCGAAAAGGCGCGACACGAAAATAGCGCTATAAAAGAGAAAATGGCTTTCACTCTCTGCAGCAGAGTTGTGACTCCATTGACATCGTCGTTTGCGTTACGACGTCGTTTACCTCTGGTTGGTGCGTTTTGCGTACTGAGCTTCGGAATCACCAATCTCTTCGTCTCCAAAACGGGTTCGGCTCTGTCTCTGCCTTTCGCTCCTCTCTTGAGATCGAAGTTTGCTGGTCAAGGACCAGCGAGGAACGTACATTTGATAAAAATGGAAGGGAGTAGTGCTGCAGTGCCTAGCATTGTTGTTTATGTCACCGTTCCTAACAGGGAAGCAGGTAAGAAACTGGCTGAAAGCATTGTCAAAGAAAAACTTGCTGCTTGTGTTAACATAGTACCTGGTATGTGCATCTTCTCAACCTCTGCTCGTCATCTTAATGTGACAGAATGCATTGCTCACGGCATAGAATCTGTTTACCAGTGGAAGGGAGAGATCCAGACAGACCCTGAGGAACTTCTGATTATTAAGACCAGGCAATCACTTCTGGGAGCACTGACAGATCATGTAAAGGCAAATCACCCATACGAGGTGCCTGAAGTTATTGCTTTGCCTATCAATGGTGGCAGCCTCGAGTACCTAGAGTGGATCAAGAGCAGCACAAAGGACTGACTTTCTCTGCGTTAACAAGTTTATTCGAGTATATCAAGTATTCTGGTTAATTTGGTATATGAACCCCATCCCCACCTTATGCTCGATGTTGTCTTGTAAATAAGAGTTGAAATTTGGTCTCTATATTGATATCTTTTGTCCTTCAATCTCCCTGCATTGGGTTTGTCAATGACTCATTCCCTAAACTTACATGGGGAAAATACATGGGGAAAAACTGCGGGAGTTTTTTTACTTAGTTCGAGGTCAGAAATCTCAACCTCAAAACTTCTCATTGCTTCACATTTGATAATGATTTCCACGAAACGATTGTTGGTCAAATGCCTTCCTGTATCGTCTTGCAAATAGCTCCATTTGGTGAAGAATTTTTATGTGAACATTTGAGACGAAAGTGTATGGATGGTATCTTCTTTTATGTGTTTTCACAAGACTTTCGTAACATCATCCCAGAATCTACCCAAAGTTTATAAGTTAG

Coding sequence (CDS)

ATGGCTTTCACTCTCTGCAGCAGAGTTGTGACTCCATTGACATCGTCGTTTGCGTTACGACGTCGTTTACCTCTGGTTGGTGCGTTTTGCGTACTGAGCTTCGGAATCACCAATCTCTTCGTCTCCAAAACGGGTTCGGCTCTGTCTCTGCCTTTCGCTCCTCTCTTGAGATCGAAGTTTGCTGGTCAAGGACCAGCGAGGAACGTACATTTGATAAAAATGGAAGGGAGTAGTGCTGCAGTGCCTAGCATTGTTGTTTATGTCACCGTTCCTAACAGGGAAGCAGGTAAGAAACTGGCTGAAAGCATTGTCAAAGAAAAACTTGCTGCTTGTGTTAACATAGTACCTGGTATGTGCATCTTCTCAACCTCTGCTCGTCATCTTAATGTGACAGAATGCATTGCTCACGGCATAGAATCTGTTTACCAGTGGAAGGGAGAGATCCAGACAGACCCTGAGGAACTTCTGATTATTAAGACCAGGCAATCACTTCTGGGAGCACTGACAGATCATGTAAAGGCAAATCACCCATACGAGGTGCCTGAAGTTATTGCTTTGCCTATCAATGGTGGCAGCCTCGAGTACCTAGAGTGGATCAAGAGCAGCACAAAGGACTGA

Protein sequence

MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKFAGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCIFSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLEYLEWIKSSTKD
Homology
BLAST of Lsi08G010080 vs. ExPASy Swiss-Prot
Match: P93009 (Protein CutA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CUTA PE=1 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.9e-51
Identity = 117/205 (57.07%), Postives = 143/205 (69.76%), Query Frame = 0

Query: 5   LCSRVVTPLTSSFALRRRLPLVGAFCVLS-FGITNLFVS---KTGSALSLPFAPLLRSKF 64
           + S + T L++    RR  P+VGAFCVLS   I++L  S   K+G A S    PLLRSKF
Sbjct: 1   MASSLTTRLSAVIGSRRSFPIVGAFCVLSTLSISSLSSSSPFKSGCAQSFSVVPLLRSKF 60

Query: 65  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 124
           + +  + +   I+ME SS  VPSIVVYVTVPNREAGKKLA SIV+EKLAACVNIVP    
Sbjct: 61  SSKAFSSS---IRMEESSKTVPSIVVYVTVPNREAGKKLANSIVQEKLAACVNIVP---- 120

Query: 125 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 184
                           GIESVY+W+G++Q+D EELLIIKTRQSLL  LT+HV ANH Y+V
Sbjct: 121 ----------------GIESVYEWEGKVQSDSEELLIIKTRQSLLEPLTEHVNANHEYDV 180

Query: 185 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPI GGS +YLEW+K+ST++
Sbjct: 181 PEVIALPITGGSDKYLEWLKNSTRN 182

BLAST of Lsi08G010080 vs. ExPASy Swiss-Prot
Match: Q109R6 (Protein CutA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CUTA1 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.5e-43
Identity = 100/191 (52.36%), Postives = 119/191 (62.30%), Query Frame = 0

Query: 15  SSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKFAGQGPARNVHLIKM 74
           S   LRRR P+ GA   LS G                        FAG     +    +M
Sbjct: 29  SPLLLRRRAPIAGALLFLSLG-----------------------AFAGVRSLSSSASARM 88

Query: 75  EGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCIFSTSARHLNVTECI 134
           E +S  VPSIVVYVTVPN+EAGK+LA SI+ EKLAACVNIVP                  
Sbjct: 89  ESTSTTVPSIVVYVTVPNKEAGKRLAGSIISEKLAACVNIVP------------------ 148

Query: 135 AHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLE 194
             GIESVY W+G++QTD EELLIIKTR+SLL ALT+HVKANH Y+VPEVIALPI GG+L+
Sbjct: 149 --GIESVYWWEGKVQTDAEELLIIKTRESLLDALTEHVKANHEYDVPEVIALPIKGGNLK 176

Query: 195 YLEWIKSSTKD 206
           YLEW+K+ST++
Sbjct: 209 YLEWLKNSTRE 176

BLAST of Lsi08G010080 vs. ExPASy Swiss-Prot
Match: O60888 (Protein CutA OS=Homo sapiens OX=9606 GN=CUTA PE=1 SV=2)

HSP 1 Score: 108.6 bits (270), Expect = 8.4e-23
Identity = 64/181 (35.36%), Postives = 96/181 (53.04%), Query Frame = 0

Query: 31  VLSFGITNLFVSKTGSALSLPFA------PLLRSKFAGQGPARNVHLIKMEGSSAAVPSI 90
           VL  G+ +L +S       LP A      P +    A   P          GS     S+
Sbjct: 9   VLLGGVASLLLSFVWMPALLPVASRLLLLPRVLLTMASGSPPTQPSPASDSGSGYVPGSV 68

Query: 91  -VVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCIFSTSARHLNVTECIAHGIESVYQ 150
              +VT PN +  K++A ++V+++LAACVN++P                     I S+Y+
Sbjct: 69  SAAFVTCPNEKVAKEIARAVVEKRLAACVNLIP--------------------QITSIYE 128

Query: 151 WKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLEYLEWIKSST 205
           WKG+I+ D E L++IKT+ SL+ ALTD V++ HPYEV EVIALP+  G+  YL+W++  T
Sbjct: 129 WKGKIEEDSEVLMMIKTQSSLVPALTDFVRSVHPYEVAEVIALPVEQGNFPYLQWVRQVT 169

BLAST of Lsi08G010080 vs. ExPASy Swiss-Prot
Match: Q6MGD0 (Protein CutA OS=Rattus norvegicus OX=10116 GN=Cuta PE=1 SV=2)

HSP 1 Score: 104.8 bits (260), Expect = 1.2e-21
Identity = 66/180 (36.67%), Postives = 97/180 (53.89%), Query Frame = 0

Query: 27  GAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKFAGQGPARNVHLIKMEGSSAAVPSIV- 86
           GA  +LSF      +      L LP A  L S  +G  P++          S  VP  V 
Sbjct: 14  GATLLLSFLCMPALLPVASRLLLLPRA--LLSMASGSPPSQP----SPASGSGYVPGSVS 73

Query: 87  -VYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCIFSTSARHLNVTECIAHGIESVYQW 146
             +VT PN +  K++A ++V+++LAACVN++P                     I S+Y+W
Sbjct: 74  AAFVTCPNEKVAKEIARAVVEKRLAACVNLIP--------------------QITSIYEW 133

Query: 147 KGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLEYLEWIKSSTK 205
           KG+I+ D E L++IKT+ SL+ ALT+ V++ HPYEV EVIALP+  G+  YL W+   T+
Sbjct: 134 KGKIEEDSEVLMMIKTQSSLVPALTEFVRSVHPYEVAEVIALPVEQGNPPYLHWVHQVTE 167

BLAST of Lsi08G010080 vs. ExPASy Swiss-Prot
Match: Q7SIA8 (Divalent-cation tolerance protein CutA OS=Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) OX=300852 GN=cutA PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.6e-21
Identity = 50/119 (42.02%), Postives = 74/119 (62.18%), Query Frame = 0

Query: 85  VVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCIFSTSARHLNVTECIAHGIESVYQW 144
           VV +TVP+ E  + +A+++V+E+LAACVNIVP                    G+ S+Y+W
Sbjct: 4   VVLITVPSEEVARTIAKALVEERLAACVNIVP--------------------GLTSIYRW 63

Query: 145 KGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEVPEVIALPINGGSLEYLEWIKSST 204
           +GE+  D E LL++KT       L + VKA HPY VPE++ALPI  G+ EYL+W++ +T
Sbjct: 64  QGEVVEDQELLLLVKTTTHAFPKLKERVKALHPYTVPEIVALPIAEGNREYLDWLRENT 102

BLAST of Lsi08G010080 vs. ExPASy TrEMBL
Match: A0A0A0LRU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370360 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 4.6e-88
Identity = 176/205 (85.85%), Postives = 179/205 (87.32%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTL SR  TPL +SFALRRRLPLVGAFCVLSFGI+NLFVSKTGSALSLPF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLITSFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSLPFVPLLRSKL 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
           AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIESVYQWKGEIQ+DPEELLIIKTRQSLLGALTDHVKANHPYEV
Sbjct: 121 ----------------GIESVYQWKGEIQSDPEELLIIKTRQSLLGALTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGSLEYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSLEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. ExPASy TrEMBL
Match: A0A1S3BBF3 (protein CutA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488107 PE=3 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 7.8e-88
Identity = 175/205 (85.37%), Postives = 178/205 (86.83%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTL SR  TPL S FALRRRLPLVGAFCVLSFGI+NLFVSKTGSALS+PF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLISCFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSVPFVPLLRSKL 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
           AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIES+YQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV
Sbjct: 121 ----------------GIESIYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGSLEYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSLEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. ExPASy TrEMBL
Match: A0A5A7VD29 (Protein CutA OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004450 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.9e-87
Identity = 175/205 (85.37%), Postives = 177/205 (86.34%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTL SR  TPL S FALRRRLPLVGAFCVLSFGI+NLFVSKTGSALSLPF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLISCFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSLPFVPLLRSKL 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
           AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIES+YQWKGEIQTDPEELLIIKTRQSLLGALTDHVKA HPYEV
Sbjct: 121 ----------------GIESIYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKAIHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGSLEYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSLEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. ExPASy TrEMBL
Match: A0A6J1K4I7 (protein CutA, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111492224 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 2.1e-85
Identity = 171/205 (83.41%), Postives = 175/205 (85.37%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTLCSRV TPL S   LRRRLPLVGAFCVLSFG +NLFVS+TGSALSLPFAPLLRS F
Sbjct: 1   MAFTLCSRVATPLLSPSVLRRRLPLVGAFCVLSFGFSNLFVSRTGSALSLPFAPLLRSTF 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
             QGPARNVHLIKMEG+SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  GSQGPARNVHLIKMEGNSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIESVYQWKGEIQTD EELLIIKTRQSLLGALTDHVKANHPYEV
Sbjct: 121 ----------------GIESVYQWKGEIQTDAEELLIIKTRQSLLGALTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGS+EYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSVEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. ExPASy TrEMBL
Match: A0A6J1BS17 (protein CutA, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005035 PE=3 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 8.9e-84
Identity = 166/205 (80.98%), Postives = 176/205 (85.85%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTLCSRV  PL SS ALRRRLPLVGAFCVLSFGI+++F+S+TGSALSLPFAPLLRSKF
Sbjct: 1   MAFTLCSRVSAPLLSSSALRRRLPLVGAFCVLSFGISHIFISRTGSALSLPFAPLLRSKF 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
            GQGPARNVH ++ME +SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  GGQGPARNVHFVRMEATSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIESVYQWKGEIQTDPEELLIIKTRQSLLG+LTDHVKANHPYEV
Sbjct: 121 ----------------GIESVYQWKGEIQTDPEELLIIKTRQSLLGSLTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIAL I GGSLEYL+WIKSSTKD
Sbjct: 181 PEVIALRITGGSLEYLDWIKSSTKD 185

BLAST of Lsi08G010080 vs. NCBI nr
Match: XP_004152609.1 (protein CutA, chloroplastic [Cucumis sativus] >KGN62736.1 hypothetical protein Csa_022362 [Cucumis sativus])

HSP 1 Score: 334.0 bits (855), Expect = 9.4e-88
Identity = 176/205 (85.85%), Postives = 179/205 (87.32%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTL SR  TPL +SFALRRRLPLVGAFCVLSFGI+NLFVSKTGSALSLPF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLITSFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSLPFVPLLRSKL 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
           AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIESVYQWKGEIQ+DPEELLIIKTRQSLLGALTDHVKANHPYEV
Sbjct: 121 ----------------GIESVYQWKGEIQSDPEELLIIKTRQSLLGALTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGSLEYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSLEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. NCBI nr
Match: XP_038886215.1 (protein CutA, chloroplastic [Benincasa hispida] >XP_038886216.1 protein CutA, chloroplastic [Benincasa hispida])

HSP 1 Score: 333.6 bits (854), Expect = 1.2e-87
Identity = 175/205 (85.37%), Postives = 177/205 (86.34%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MA T CSR  +PL SSF LRRRLPLVGAFCVLSFGI+NLFVSKTGSALSLPF PLLRSKF
Sbjct: 1   MALTFCSRATSPLISSFVLRRRLPLVGAFCVLSFGISNLFVSKTGSALSLPFVPLLRSKF 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
           A QGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  ASQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV
Sbjct: 121 ----------------GIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGSLEYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSLEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. NCBI nr
Match: XP_008444893.1 (PREDICTED: protein CutA, chloroplastic [Cucumis melo])

HSP 1 Score: 333.2 bits (853), Expect = 1.6e-87
Identity = 175/205 (85.37%), Postives = 178/205 (86.83%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTL SR  TPL S FALRRRLPLVGAFCVLSFGI+NLFVSKTGSALS+PF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLISCFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSVPFVPLLRSKL 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
           AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIES+YQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV
Sbjct: 121 ----------------GIESIYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGSLEYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSLEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. NCBI nr
Match: KAA0065110.1 (protein CutA [Cucumis melo var. makuwa])

HSP 1 Score: 330.9 bits (847), Expect = 8.0e-87
Identity = 175/205 (85.37%), Postives = 177/205 (86.34%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTL SR  TPL S FALRRRLPLVGAFCVLSFGI+NLFVSKTGSALSLPF PLLRSK 
Sbjct: 1   MAFTLSSRAATPLISCFALRRRLPLVGAFCVLSFGISNLFVSKTGSALSLPFVPLLRSKL 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
           AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIES+YQWKGEIQTDPEELLIIKTRQSLLGALTDHVKA HPYEV
Sbjct: 121 ----------------GIESIYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKAIHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGSLEYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSLEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. NCBI nr
Match: XP_023545579.1 (protein CutA, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 326.6 bits (836), Expect = 1.5e-85
Identity = 172/205 (83.90%), Postives = 175/205 (85.37%), Query Frame = 0

Query: 1   MAFTLCSRVVTPLTSSFALRRRLPLVGAFCVLSFGITNLFVSKTGSALSLPFAPLLRSKF 60
           MAFTLCSRV TPL S   LRRRLPLVGAFCVLSFG +NLFVSKTGSALSLPFAPLLRS F
Sbjct: 1   MAFTLCSRVATPLLSPSVLRRRLPLVGAFCVLSFGFSNLFVSKTGSALSLPFAPLLRSTF 60

Query: 61  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 120
             QGPARNVHLIKMEG+SAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP    
Sbjct: 61  GSQGPARNVHLIKMEGNSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVP---- 120

Query: 121 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 180
                           GIESVYQWKGEIQTD EELLIIKTRQSLLGALTDHVKANHPYEV
Sbjct: 121 ----------------GIESVYQWKGEIQTDAEELLIIKTRQSLLGALTDHVKANHPYEV 180

Query: 181 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPINGGS+EYLEWIKSSTKD
Sbjct: 181 PEVIALPINGGSMEYLEWIKSSTKD 185

BLAST of Lsi08G010080 vs. TAIR 10
Match: AT2G33740.2 (Nitrogen regulatory PII-like, alpha/beta )

HSP 1 Score: 203.8 bits (517), Expect = 1.4e-52
Identity = 117/205 (57.07%), Postives = 143/205 (69.76%), Query Frame = 0

Query: 5   LCSRVVTPLTSSFALRRRLPLVGAFCVLS-FGITNLFVS---KTGSALSLPFAPLLRSKF 64
           + S + T L++    RR  P+VGAFCVLS   I++L  S   K+G A S    PLLRSKF
Sbjct: 1   MASSLTTRLSAVIGSRRSFPIVGAFCVLSTLSISSLSSSSPFKSGCAQSFSVVPLLRSKF 60

Query: 65  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 124
           + +  + +   I+ME SS  VPSIVVYVTVPNREAGKKLA SIV+EKLAACVNIVP    
Sbjct: 61  SSKAFSSS---IRMEESSKTVPSIVVYVTVPNREAGKKLANSIVQEKLAACVNIVP---- 120

Query: 125 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYEV 184
                           GIESVY+W+G++Q+D EELLIIKTRQSLL  LT+HV ANH Y+V
Sbjct: 121 ----------------GIESVYEWEGKVQSDSEELLIIKTRQSLLEPLTEHVNANHEYDV 180

Query: 185 PEVIALPINGGSLEYLEWIKSSTKD 206
           PEVIALPI GGS +YLEW+K+ST++
Sbjct: 181 PEVIALPITGGSDKYLEWLKNSTRN 182

BLAST of Lsi08G010080 vs. TAIR 10
Match: AT2G33740.1 (Nitrogen regulatory PII-like, alpha/beta )

HSP 1 Score: 163.7 bits (413), Expect = 1.6e-40
Identity = 99/179 (55.31%), Postives = 119/179 (66.48%), Query Frame = 0

Query: 5   LCSRVVTPLTSSFALRRRLPLVGAFCVLS-FGITNLFVS---KTGSALSLPFAPLLRSKF 64
           + S + T L++    RR  P+VGAFCVLS   I++L  S   K+G A S    PLLRSKF
Sbjct: 1   MASSLTTRLSAVIGSRRSFPIVGAFCVLSTLSISSLSSSSPFKSGCAQSFSVVPLLRSKF 60

Query: 65  AGQGPARNVHLIKMEGSSAAVPSIVVYVTVPNREAGKKLAESIVKEKLAACVNIVPGMCI 124
           + +  + +   I+ME SS  VPSIVVYVTVPNREAGKKLA SIV+EKLAACVNIVP    
Sbjct: 61  SSKAFSSS---IRMEESSKTVPSIVVYVTVPNREAGKKLANSIVQEKLAACVNIVP---- 120

Query: 125 FSTSARHLNVTECIAHGIESVYQWKGEIQTDPEELLIIKTRQSLLGALTDHVKANHPYE 180
                           GIESVY+W+G++Q+D EELLIIKTRQSLL  LT+HV ANH YE
Sbjct: 121 ----------------GIESVYEWEGKVQSDSEELLIIKTRQSLLEPLTEHVNANHEYE 156

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P930091.9e-5157.07Protein CutA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CUTA PE=1 SV=1[more]
Q109R61.5e-4352.36Protein CutA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CUTA1 ... [more]
O608888.4e-2335.36Protein CutA OS=Homo sapiens OX=9606 GN=CUTA PE=1 SV=2[more]
Q6MGD01.2e-2136.67Protein CutA OS=Rattus norvegicus OX=10116 GN=Cuta PE=1 SV=2[more]
Q7SIA81.6e-2142.02Divalent-cation tolerance protein CutA OS=Thermus thermophilus (strain ATCC 2763... [more]
Match NameE-valueIdentityDescription
A0A0A0LRU04.6e-8885.85Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G370360 PE=3 SV=1[more]
A0A1S3BBF37.8e-8885.37protein CutA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103488107 PE=3 SV=1[more]
A0A5A7VD293.9e-8785.37Protein CutA OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004450 P... [more]
A0A6J1K4I72.1e-8583.41protein CutA, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111492224 PE=3 SV=... [more]
A0A6J1BS178.9e-8480.98protein CutA, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005035 PE=3 ... [more]
Match NameE-valueIdentityDescription
XP_004152609.19.4e-8885.85protein CutA, chloroplastic [Cucumis sativus] >KGN62736.1 hypothetical protein C... [more]
XP_038886215.11.2e-8785.37protein CutA, chloroplastic [Benincasa hispida] >XP_038886216.1 protein CutA, ch... [more]
XP_008444893.11.6e-8785.37PREDICTED: protein CutA, chloroplastic [Cucumis melo][more]
KAA0065110.18.0e-8785.37protein CutA [Cucumis melo var. makuwa][more]
XP_023545579.11.5e-8583.90protein CutA, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G33740.21.4e-5257.07Nitrogen regulatory PII-like, alpha/beta [more]
AT2G33740.11.6e-4055.31Nitrogen regulatory PII-like, alpha/beta [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004323Divalent ion tolerance protein, CutAPFAMPF03091CutA1coord: 84..201
e-value: 7.2E-33
score: 112.4
IPR004323Divalent ion tolerance protein, CutAPANTHERPTHR23419DIVALENT CATION TOLERANCE CUTA-RELATEDcoord: 134..203
coord: 13..119
IPR015867Nitrogen regulatory protein PII/ATP phosphoribosyltransferase, C-terminalGENE3D3.30.70.120coord: 74..205
e-value: 4.8E-43
score: 147.3
NoneNo IPR availablePANTHERPTHR23419:SF8FI09726Pcoord: 134..203
coord: 13..119
IPR011322Nitrogen regulatory PII-like, alpha/betaSUPERFAMILY54913GlnB-likecoord: 83..204

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G010080.1Lsi08G010080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010038 response to metal ion
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding