Lsi08G009920 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G009920
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	ENTH domain-containing protein
Location	chr08: 18391061 .. 18392134 (+)
RNA-Seq Expression	Lsi08G009920
Synteny	Lsi08G009920

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATAGACCAAAACAAGAAGCTCAAGAATCTCATACACACTCTCAAAGACAAGGCCTCAATAATCAAAGCTACTTTCTCGATTCATCGCCGTTCATCTTCCATCAAAGTCGCCGTCGTCCGGGCCACCACCCACGGTTCTCGAAACCCACCTTCCGACGGCCGAGTCGCCGCCGTGCTAGCCCTTGGGAATGACTTCCGTTCCTCGACTGCATTCGCCTGCATCGAAGCCCTAATGGAGCGGCTTCATACGACCTCTAGCGCCGCCGTGGCTATCAAATCGCTTTTCACTCTGCATATAATTGTAATTCGAGGTCCGTTCAATCTGAGGGATCAGGTGGCGTTTTTCCCCTGTTACGGAGGGCGAAATTTTCTCAACTTATCCGCGTTTCGCGACGTATCGGACTCGGAGATGAACGACTTGTCGTCTTGGGTGAGATGGTACGCGGGGGTTGTGGAGCACAACGTGATTGTTGACAGGAAATTGGATCGAATTCTGTATTTCCGTTCAAGAAATTGCGAAATTGTTGAAGATCATAAAGAAGGGAGGAAGAGGAAGATTGATTTATCCGAGGAATTGGAGGTTCTTGTGAGTTTTGTAGAGAGAATTTGCGAAGTTCCAGAATCGCTGCATCTTCAGAAGAAGGATTTGGTTTACGAGGTGGTTAAATTGGTTCTTGAGAATTACAGGTTGGTTCAGAGGGAGATTTGGGTCCGAGTTAAAGAAATCGGAGACAGAGTCGATAGTTTGAGTCTGGACGAGTTGACTGAGTTGGTGGGTATTTTGAGACGGTTGGAAAATTGCAGAAGGAACTTGAGTGTGTTGTTTGTAAACAGAGGGAAGAACGAGGAATTTTGGGAATTGGTGAAAATTACGAAAGGGAAACTGGCGGAGACGAAGGGGATGAAAGAGGAGAAGAGGATGATAATGGTGGAGATGAGAGCGGACTCGGTCGAGTCGACTCGGTTCTGGAATCCATTTGTTGAACCGGGTCAATTGCTGTGGGTCCCAGCGGGTGATGGACACTTGGGCCCGGCTCCGCTTCCACTGACCGTTTCAACGGTAGGATAG

mRNA sequence

ATGGCCATAGACCAAAACAAGAAGCTCAAGAATCTCATACACACTCTCAAAGACAAGGCCTCAATAATCAAAGCTACTTTCTCGATTCATCGCCGTTCATCTTCCATCAAAGTCGCCGTCGTCCGGGCCACCACCCACGGTTCTCGAAACCCACCTTCCGACGGCCGAGTCGCCGCCGTGCTAGCCCTTGGGAATGACTTCCGTTCCTCGACTGCATTCGCCTGCATCGAAGCCCTAATGGAGCGGCTTCATACGACCTCTAGCGCCGCCGTGGCTATCAAATCGCTTTTCACTCTGCATATAATTGTAATTCGAGGTCCGTTCAATCTGAGGGATCAGGTGGCGTTTTTCCCCTGTTACGGAGGGCGAAATTTTCTCAACTTATCCGCGTTTCGCGACGTATCGGACTCGGAGATGAACGACTTGTCGTCTTGGGTGAGATGGTACGCGGGGGTTGTGGAGCACAACGTGATTGTTGACAGGAAATTGGATCGAATTCTGTATTTCCGTTCAAGAAATTGCGAAATTGTTGAAGATCATAAAGAAGGGAGGAAGAGGAAGATTGATTTATCCGAGGAATTGGAGGTTCTTGTGAGTTTTGTAGAGAGAATTTGCGAAGTTCCAGAATCGCTGCATCTTCAGAAGAAGGATTTGGTTTACGAGGTGGTTAAATTGGTTCTTGAGAATTACAGGTTGGTTCAGAGGGAGATTTGGGTCCGAGTTAAAGAAATCGGAGACAGAGTCGATAGTTTGAGTCTGGACGAGTTGACTGAGTTGGTGGGTATTTTGAGACGGTTGGAAAATTGCAGAAGGAACTTGAGTGTGTTGTTTGTAAACAGAGGGAAGAACGAGGAATTTTGGGAATTGGTGAAAATTACGAAAGGGAAACTGGCGGAGACGAAGGGGATGAAAGAGGAGAAGAGGATGATAATGGTGGAGATGAGAGCGGACTCGGTCGAGTCGACTCGGTTCTGGAATCCATTTGTTGAACCGGGTCAATTGCTGTGGGTCCCAGCGGGTGATGGACACTTGGGCCCGGCTCCGCTTCCACTGACCGTTTCAACGGTAGGATAG

Coding sequence (CDS)

ATGGCCATAGACCAAAACAAGAAGCTCAAGAATCTCATACACACTCTCAAAGACAAGGCCTCAATAATCAAAGCTACTTTCTCGATTCATCGCCGTTCATCTTCCATCAAAGTCGCCGTCGTCCGGGCCACCACCCACGGTTCTCGAAACCCACCTTCCGACGGCCGAGTCGCCGCCGTGCTAGCCCTTGGGAATGACTTCCGTTCCTCGACTGCATTCGCCTGCATCGAAGCCCTAATGGAGCGGCTTCATACGACCTCTAGCGCCGCCGTGGCTATCAAATCGCTTTTCACTCTGCATATAATTGTAATTCGAGGTCCGTTCAATCTGAGGGATCAGGTGGCGTTTTTCCCCTGTTACGGAGGGCGAAATTTTCTCAACTTATCCGCGTTTCGCGACGTATCGGACTCGGAGATGAACGACTTGTCGTCTTGGGTGAGATGGTACGCGGGGGTTGTGGAGCACAACGTGATTGTTGACAGGAAATTGGATCGAATTCTGTATTTCCGTTCAAGAAATTGCGAAATTGTTGAAGATCATAAAGAAGGGAGGAAGAGGAAGATTGATTTATCCGAGGAATTGGAGGTTCTTGTGAGTTTTGTAGAGAGAATTTGCGAAGTTCCAGAATCGCTGCATCTTCAGAAGAAGGATTTGGTTTACGAGGTGGTTAAATTGGTTCTTGAGAATTACAGGTTGGTTCAGAGGGAGATTTGGGTCCGAGTTAAAGAAATCGGAGACAGAGTCGATAGTTTGAGTCTGGACGAGTTGACTGAGTTGGTGGGTATTTTGAGACGGTTGGAAAATTGCAGAAGGAACTTGAGTGTGTTGTTTGTAAACAGAGGGAAGAACGAGGAATTTTGGGAATTGGTGAAAATTACGAAAGGGAAACTGGCGGAGACGAAGGGGATGAAAGAGGAGAAGAGGATGATAATGGTGGAGATGAGAGCGGACTCGGTCGAGTCGACTCGGTTCTGGAATCCATTTGTTGAACCGGGTCAATTGCTGTGGGTCCCAGCGGGTGATGGACACTTGGGCCCGGCTCCGCTTCCACTGACCGTTTCAACGGTAGGATAG

Protein sequence

MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAVLALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDHKEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVRVKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAETKGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG

Homology

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BLAST of Lsi08G009920 vs. ExPASy Swiss-Prot
Match: Q8L936 (Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana OX=3702 GN=At4g40080 PE=2 SV=2)

HSP 1 Score: 176.0 bits (445), Expect = 7.5e-43
Identity = 123/328 (37.50%), Postives = 179/328 (54.57%), Query Frame = 0


Query: 11  NLIHTLKDKASIIKATF---SIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAVLALGNDF 70
           +LI  +KDKAS  KA     +   ++ S  ++V+RATTH    PP +  +A +L+ G   
Sbjct: 9   DLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAVILSAGTGS 68

Query: 71  RSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGGRNFLN 130
           R +TA + +E++MERLHTT  A VA+KSL  +H IV  G F L+DQ++ FP  GGRN+L 
Sbjct: 69  R-ATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPASGGRNYLK 128

Query: 131 LSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDHKEGRKRK 190
           LSAFRD     M +LSSWVRWYA  +EH +   R +    +F S     +  HKE  +  
Sbjct: 129 LSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMG---FFISSTSSTI--HKEEYEEM 188

Query: 191 I------DLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVRV 250
           +      DL  E++ LV  +E  C++P+      K L  ++ +LV E+Y     E++ R 
Sbjct: 189 VSSLTNSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYTRF 248

Query: 251 KEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLF---VNRGKNEEFWELVKITKGKLA 310
            E  +R ++LS  +  ELV  L+RLE+C+  LS +      RG  + FW LV   KG + 
Sbjct: 249 NEFKERSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWGLVLEVKGIIG 308

Query: 311 --ETKGMKEEKRMIMVEMRADSVESTRF 325
             E    + EK ++    R    ES RF
Sbjct: 309 NLEDNYGQIEKSIVGFGKRDKGYESARF 330

[Close]

BLAST of Lsi08G009920 vs. ExPASy Swiss-Prot
Match: Q8H0W9 (Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana OX=3702 GN=At5g10410 PE=2 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 7.5e-19
Identity = 97/313 (30.99%), Postives = 158/313 (50.48%), Query Frame = 0


Query: 11  NLIHTLKDKASIIKATFSIHRRSSSIK---VAVVRATTHGSRNPPSDGRVAAVLALGNDF 70
           ++I   KDKASI KA       S+++K   +A++++TT     PP+   V+AV++  N  
Sbjct: 7   HIIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISYSNSR 66

Query: 71  RSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGGRNFLN 130
            +  AF+   A + RL  T +A VA KSL  +H ++     + RD+  F     GRN L 
Sbjct: 67  YAPAAFS---AALWRLRVTKNAIVATKSLIVIHKLI----KSSRDK--FEGLGHGRNNLK 126

Query: 131 LSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDHKEGRKRK 190
           L+ F D S +   +LS W+RWY   ++    V + L           + VE+       +
Sbjct: 127 LNEFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQ 186

Query: 191 ID-LSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVRVKEIGD 250
              +  + + LVSF E IC  PE   + +  +V E+ +LV+E+Y  + R + VR++ + +
Sbjct: 187 TGYIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFE 246

Query: 251 R--------VDSLSLDELTELVGILRRLENCRRNLSVLFVN-RGKNEEFWELVKITKGKL 310
           R        +  L L++ + L   L RL  C+ +LS LF   R   ++FW LV++ K   
Sbjct: 247 RLIKPGVKPIGDLGLNDFSLL---LVRLVECKESLSGLFWRCRRLADDFWCLVEMLK--- 303

[Close]

BLAST of Lsi08G009920 vs. ExPASy Swiss-Prot
Match: Q9FKQ2 (Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana OX=3702 GN=At5g65370 PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.9e-14
Identity = 88/307 (28.66%), Postives = 145/307 (47.23%), Query Frame = 0


Query: 8   KLKNLIHTLKDKASIIKATFSIHRRSS----SIKVAVVRATTHGSRNPPSDGRVAAVLAL 67
           KL  L   LKD+AS +K    +H  SS    +I +A+++AT+H S NPPSD  V      
Sbjct: 3   KLATLNGILKDEASQMKLNV-VHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVT----- 62

Query: 68  GNDFRSSTAFAC-----IEALMERLHTTSSAAVAIKSLFTLHIIV-----IRGPFNLRDQ 127
              F  ST   C     ++A++ RL  T+   VA K L  LH +V       G  +LR+ 
Sbjct: 63  ---FLQSTIDTCYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNN 122

Query: 128 V---AFFPCYGGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFR 187
           +         GG N L L+     S     +L+ WV+WY   ++  + +   L      +
Sbjct: 123 INHRTLIYTQGGSN-LKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIK 182

Query: 188 SRNCEIVEDHKEGRKRKID-LSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLEN 247
            +N +   + +      +D + ++++ LV   E I + P++   +   +V E+ +L++++
Sbjct: 183 EKNEDKRLETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELMVQD 242

Query: 248 YRLVQREIWVRVKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKN--EEFW 295
           Y    R + +R +E+  RV      +  ELV +L +LENC+  LS  F  R K    +FW
Sbjct: 243 YFSAIRLMRIRFEELNVRV-----AKPNELVPVLEKLENCKEGLSE-FSWRSKYLIADFW 293

[Close]

BLAST of Lsi08G009920 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 8.4e-10
Identity = 55/162 (33.95%), Postives = 86/162 (53.09%), Query Frame = 0


Query: 6   NKKLKNLIHTLKDKASIIKATFSIHRRSSS---IKVAVVRATTHGSRNPPSDGRVAAVLA 65
           + KLK  I  +KD+ S+  A   +  RSSS   +++AVV+AT H    P  D  +  +L 
Sbjct: 3   SSKLKRAIGAVKDQTSVGLA--KVGGRSSSLTELEIAVVKATRHDD-YPAEDKYIREILC 62

Query: 66  LGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGG 125
           L +  R+  + AC+  L  RL+ T + +VA+K+L  +  ++  G     +Q  FF    G
Sbjct: 63  LTSYSRNYVS-ACVATLSRRLNKTKNWSVALKTLILIQRLLTDGD-RAYEQEIFFATRRG 122

Query: 126 RNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLD 165
              LN+S FRD S S+  D S++VR YA      + +D +LD
Sbjct: 123 TRLLNMSDFRDASQSDSWDYSAFVRTYA------LYLDERLD 153

[Close]

BLAST of Lsi08G009920 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.6e-08
Identity = 53/162 (32.72%), Postives = 82/162 (50.62%), Query Frame = 0


Query: 6   NKKLKNLIHTLKDKASIIKATFSIHRRSSSIK---VAVVRATTHGSRNPPSDGRVAAVLA 65
           + K K  I  +KD+ S+  A   ++ RS+S+    VA+V+AT H    P  +  +  +L+
Sbjct: 3   SSKFKRAIGAVKDQTSVGLA--KVNGRSASLSELDVAIVKATRH-EEFPAEEKYIREILS 62

Query: 66  LGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGG 125
           L   +  S   AC+  L  RL+ T    VA+K+L  +  ++  G     +Q  FF    G
Sbjct: 63  L-TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGD-QAYEQEIFFATRRG 122

Query: 126 RNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLD 165
              LN+S FRDVS S   D S++VR YA      + +D +LD
Sbjct: 123 TRLLNMSDFRDVSRSNSWDYSAFVRTYA------LYLDERLD 153

[Close]

BLAST of Lsi08G009920 vs. ExPASy TrEMBL
Match: A0A5A7VCW1 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004590 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.6e-166
Identity = 310/357 (86.83%), Postives = 328/357 (91.88%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAI Q+KKLKNL H LKDKASIIKA FSI+RRSSSIKVAVVRATTHG+RNPPSD RVAAV
Sbjct: 1   MAIHQSKKLKNLTHALKDKASIIKANFSINRRSSSIKVAVVRATTHGARNPPSDARVAAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDFRSSTAFACIEALM RLHTTSSAAVA+KSLFTLHIIVIRGPFNLRDQV+FFP Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI E  
Sbjct: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIDEG- 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
             GRK K+DL+EEL VLV FVERICEVPESLHLQKKDLVYEVV+LVLENYRLVQREIWVR
Sbjct: 181 --GRKWKVDLAEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLENYRLVQREIWVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           VKEIG+RV+ LS+DEL+ELVGIL RLENCR  +SVLFVNRGKNEEFWELVKITKGK+AE 
Sbjct: 241 VKEIGERVERLSVDELSELVGILIRLENCRWKVSVLFVNRGKNEEFWELVKITKGKVAEK 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K +KEEKRM+MV    DSVESTR WNPFVEPGQL+WVP GDG +GPA LPLTVSTVG
Sbjct: 301 KRLKEEKRMVMV---VDSVESTRLWNPFVEPGQLVWVPGGDGPMGPALLPLTVSTVG 351

[Close]

BLAST of Lsi08G009920 vs. ExPASy TrEMBL
Match: A0A1S3BDW7 (putative clathrin assembly protein At4g40080 OS=Cucumis melo OX=3656 GN=LOC103488553 PE=4 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.6e-166
Identity = 310/357 (86.83%), Postives = 328/357 (91.88%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAI Q+KKLKNL H LKDKASIIKA FSI+RRSSSIKVAVVRATTHG+RNPPSD RVAAV
Sbjct: 1   MAIHQSKKLKNLTHALKDKASIIKANFSINRRSSSIKVAVVRATTHGARNPPSDARVAAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDFRSSTAFACIEALM RLHTTSSAAVA+KSLFTLHIIVIRGPFNLRDQV+FFP Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI E  
Sbjct: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIDEG- 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
             GRK K+DL+EEL VLV FVERICEVPESLHLQKKDLVYEVV+LVLENYRLVQREIWVR
Sbjct: 181 --GRKWKVDLAEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLENYRLVQREIWVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           VKEIG+RV+ LS+DEL+ELVGIL RLENCR  +SVLFVNRGKNEEFWELVKITKGK+AE 
Sbjct: 241 VKEIGERVERLSVDELSELVGILIRLENCRWKVSVLFVNRGKNEEFWELVKITKGKVAEK 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K +KEEKRM+MV    DSVESTR WNPFVEPGQL+WVP GDG +GPA LPLTVSTVG
Sbjct: 301 KRLKEEKRMVMV---VDSVESTRLWNPFVEPGQLVWVPGGDGPMGPALLPLTVSTVG 351

[Close]

BLAST of Lsi08G009920 vs. ExPASy TrEMBL
Match: A0A0A0LLA1 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369220 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 8.6e-159
Identity = 301/357 (84.31%), Postives = 323/357 (90.48%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAI QNKKL NL+H LKDKAS+IKATFSI+RRSSSIKVAVVRATTHG+RNPPSD RV+AV
Sbjct: 1   MAIHQNKKLNNLLHALKDKASLIKATFSINRRSSSIKVAVVRATTHGARNPPSDARVSAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDFRSSTAFACIEALM RLHTTSSAAVA+KSLFTLHIIVIRGPFNLRDQV+FFP Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEM+DLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI ED 
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIDED- 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
             GRK K+DLSEEL VLV FVERICEVPESLHLQKKDLVYEVV+LVL+NYRLVQ+EIWVR
Sbjct: 181 --GRKGKVDLSEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLQNYRLVQKEIWVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           VKEIG+RV+ LS+DEL+ELVGIL RLENCR  +SVLFVNRGK+EEFWELVK T+GKL E 
Sbjct: 241 VKEIGERVERLSVDELSELVGILTRLENCRWKVSVLFVNRGKSEEFWELVKKTRGKLGEK 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K +KEEKRMIMV    +SVESTR  NPFVEPGQL+WVP      GPA LPLTVSTVG
Sbjct: 301 KRLKEEKRMIMV---VESVESTRLRNPFVEPGQLMWVPG-----GPALLPLTVSTVG 346

[Close]

BLAST of Lsi08G009920 vs. ExPASy TrEMBL
Match: A0A6J1BR25 (putative clathrin assembly protein At4g40080 OS=Momordica charantia OX=3673 GN=LOC111004659 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 1.2e-147
Identity = 279/357 (78.15%), Postives = 307/357 (85.99%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           M IDQ KKLKNLI  LKDKASIIKATFS HRRSSSIK+AVVRATTH   NPPSD R+AAV
Sbjct: 1   MGIDQKKKLKNLIDALKDKASIIKATFSTHRRSSSIKLAVVRATTHDPSNPPSDRRLAAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDF  STA ACI+ +M+RLHTTSSA VA+KSLFTLHI+VIRGPF+LRDQV F P Y
Sbjct: 61  LALGNDFGRSTAIACIQTIMDRLHTTSSAVVAMKSLFTLHIVVIRGPFDLRDQVVFCPYY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEM+DLSSWVRWYAGVVEHNVIV R+LDRILY RS NC+I  + 
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVRRRLDRILYLRSGNCKI--ED 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
           K+G+  ++DL  EL+VLV FVE ICE P+SLHLQK ++VYEVV+LVLENYRLVQREI VR
Sbjct: 181 KQGKISELDLWGELDVLVGFVEGICEFPDSLHLQKNEMVYEVVRLVLENYRLVQREISVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           V+ IGDR DSLSLDELT+LV IL R ENCRR L+VLFVNR KNE+ WELVK TK KL E 
Sbjct: 241 VRGIGDRADSLSLDELTQLVVILTRFENCRRKLTVLFVNRAKNEDLWELVKNTKAKLVEQ 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K MKEEKRMIMVE+RA+SVE TR WNPFVEPGQLLWVP+GD  LGPA LPLTVSTVG
Sbjct: 301 KQMKEEKRMIMVEIRAESVELTRLWNPFVEPGQLLWVPSGDEPLGPALLPLTVSTVG 355

[Close]

BLAST of Lsi08G009920 vs. ExPASy TrEMBL
Match: A0A6J1HF54 (putative clathrin assembly protein At4g40080 OS=Cucurbita moschata OX=3662 GN=LOC111462893 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 2.0e-131
Identity = 267/358 (74.58%), Postives = 290/358 (81.01%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAIDQ KKLKNLI   KD+ASIIKATFSIHRRSSSIKVAVVRATTHG+RNPPSD R+AA+
Sbjct: 1   MAIDQTKKLKNLIDAFKDQASIIKATFSIHRRSSSIKVAVVRATTHGARNPPSDARLAAL 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LA GNDFRSSTAF CI+ALMERLHTT+SAAVA+KSLFTLHII IRGPFNL+ +VAF P Y
Sbjct: 61  LAFGNDFRSSTAFVCIQALMERLHTTTSAAVAMKSLFTLHIIAIRGPFNLKGEVAFSPYY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRD+SDSEM++LS WVRWYAGVVEHN    RKLDRILYFRSRN EIV   
Sbjct: 121 GGRNFLNLSAFRDLSDSEMSELSCWVRWYAGVVEHN----RKLDRILYFRSRNPEIV--- 180

Query: 181 KEGRKRKI-DLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWV 240
            EG+ RKI +L EEL+VLV F ERI EVPESLH+QK DLVYEVV+LVLE+YRLVQREIWV
Sbjct: 181 -EGKDRKIPELLEELDVLVGFSERISEVPESLHVQKSDLVYEVVRLVLESYRLVQREIWV 240

Query: 241 RVKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAE 300
           RV EIG+RV+ LS DELTE V IL R+ENCR  +SVLFVNRGKNEE WELV  TKGKL  
Sbjct: 241 RVNEIGNRVEWLSRDELTESVEILNRMENCRGKVSVLFVNRGKNEELWELVTCTKGKLV- 300

Query: 301 TKGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
                 E+R  M  M     ESTR WNPFVEPG L         LGPA LPLTVSTVG
Sbjct: 301 ------ERRRRMTTM----TESTRLWNPFVEPGSL-------RPLGPALLPLTVSTVG 332

[Close]

BLAST of Lsi08G009920 vs. NCBI nr
Match: XP_038884022.1 (putative clathrin assembly protein At4g40080 [Benincasa hispida])

HSP 1 Score: 624.8 bits (1610), Expect = 4.6e-175
Identity = 326/357 (91.32%), Postives = 336/357 (94.12%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAIDQNKKLKNL H LKDKASIIKAT SI RRSSSIKVAVVRATTHGSRNPPSD RVAAV
Sbjct: 1   MAIDQNKKLKNLTHALKDKASIIKATLSIPRRSSSIKVAVVRATTHGSRNPPSDARVAAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDFRSSTAFACIEALMERLHTTSSAAVA+KSLFTLHIIVIRGPFNLRDQVA+FPCY
Sbjct: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAYFPCY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLS FRDVSDSEMNDLSSWVRWYAGVVE NVIVDRKLDRILYFRSRNCEIVE  
Sbjct: 121 GGRNFLNLSTFRDVSDSEMNDLSSWVRWYAGVVESNVIVDRKLDRILYFRSRNCEIVE-- 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
            E RKRKID+ EELEVLV FVERICEVPESL+LQKKDLVYEVV+LVLENYRLVQREIWVR
Sbjct: 181 -EQRKRKIDVPEELEVLVGFVERICEVPESLYLQKKDLVYEVVRLVLENYRLVQREIWVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           VKEIGDRV+SLSLDELTELVGI+ RLENCRR LSVLFVNRGKNEEFWELVKITKGKLAE 
Sbjct: 241 VKEIGDRVESLSLDELTELVGIMTRLENCRRKLSVLFVNRGKNEEFWELVKITKGKLAEK 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K MKEEKRMIMVEM+A+S ESTR WNPFVEPGQLLWVPAGDG +GPA LPLTVSTVG
Sbjct: 301 KRMKEEKRMIMVEMKANSGESTRLWNPFVEPGQLLWVPAGDGPMGPALLPLTVSTVG 354

[Close]

BLAST of Lsi08G009920 vs. NCBI nr
Match: XP_008445571.1 (PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis melo] >KAA0065124.1 putative clathrin assembly protein [Cucumis melo var. makuwa])

HSP 1 Score: 593.6 bits (1529), Expect = 1.1e-165
Identity = 310/357 (86.83%), Postives = 328/357 (91.88%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAI Q+KKLKNL H LKDKASIIKA FSI+RRSSSIKVAVVRATTHG+RNPPSD RVAAV
Sbjct: 1   MAIHQSKKLKNLTHALKDKASIIKANFSINRRSSSIKVAVVRATTHGARNPPSDARVAAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDFRSSTAFACIEALM RLHTTSSAAVA+KSLFTLHIIVIRGPFNLRDQV+FFP Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI E  
Sbjct: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIDEG- 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
             GRK K+DL+EEL VLV FVERICEVPESLHLQKKDLVYEVV+LVLENYRLVQREIWVR
Sbjct: 181 --GRKWKVDLAEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLENYRLVQREIWVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           VKEIG+RV+ LS+DEL+ELVGIL RLENCR  +SVLFVNRGKNEEFWELVKITKGK+AE 
Sbjct: 241 VKEIGERVERLSVDELSELVGILIRLENCRWKVSVLFVNRGKNEEFWELVKITKGKVAEK 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K +KEEKRM+MV    DSVESTR WNPFVEPGQL+WVP GDG +GPA LPLTVSTVG
Sbjct: 301 KRLKEEKRMVMV---VDSVESTRLWNPFVEPGQLVWVPGGDGPMGPALLPLTVSTVG 351

[Close]

BLAST of Lsi08G009920 vs. NCBI nr
Match: XP_004152749.1 (putative clathrin assembly protein At4g40080 [Cucumis sativus] >KGN62720.1 hypothetical protein Csa_022647 [Cucumis sativus])

HSP 1 Score: 569.7 bits (1467), Expect = 1.8e-158
Identity = 301/357 (84.31%), Postives = 323/357 (90.48%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAI QNKKL NL+H LKDKAS+IKATFSI+RRSSSIKVAVVRATTHG+RNPPSD RV+AV
Sbjct: 1   MAIHQNKKLNNLLHALKDKASLIKATFSINRRSSSIKVAVVRATTHGARNPPSDARVSAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDFRSSTAFACIEALM RLHTTSSAAVA+KSLFTLHIIVIRGPFNLRDQV+FFP Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEM+DLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI ED 
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIDED- 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
             GRK K+DLSEEL VLV FVERICEVPESLHLQKKDLVYEVV+LVL+NYRLVQ+EIWVR
Sbjct: 181 --GRKGKVDLSEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLQNYRLVQKEIWVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           VKEIG+RV+ LS+DEL+ELVGIL RLENCR  +SVLFVNRGK+EEFWELVK T+GKL E 
Sbjct: 241 VKEIGERVERLSVDELSELVGILTRLENCRWKVSVLFVNRGKSEEFWELVKKTRGKLGEK 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K +KEEKRMIMV    +SVESTR  NPFVEPGQL+WVP      GPA LPLTVSTVG
Sbjct: 301 KRLKEEKRMIMV---VESVESTRLRNPFVEPGQLMWVPG-----GPALLPLTVSTVG 346

[Close]

BLAST of Lsi08G009920 vs. NCBI nr
Match: XP_022131457.1 (putative clathrin assembly protein At4g40080 [Momordica charantia])

HSP 1 Score: 532.7 bits (1371), Expect = 2.4e-147
Identity = 279/357 (78.15%), Postives = 307/357 (85.99%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           M IDQ KKLKNLI  LKDKASIIKATFS HRRSSSIK+AVVRATTH   NPPSD R+AAV
Sbjct: 1   MGIDQKKKLKNLIDALKDKASIIKATFSTHRRSSSIKLAVVRATTHDPSNPPSDRRLAAV 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LALGNDF  STA ACI+ +M+RLHTTSSA VA+KSLFTLHI+VIRGPF+LRDQV F P Y
Sbjct: 61  LALGNDFGRSTAIACIQTIMDRLHTTSSAVVAMKSLFTLHIVVIRGPFDLRDQVVFCPYY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEM+DLSSWVRWYAGVVEHNVIV R+LDRILY RS NC+I  + 
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVRRRLDRILYLRSGNCKI--ED 180

Query: 181 KEGRKRKIDLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVR 240
           K+G+  ++DL  EL+VLV FVE ICE P+SLHLQK ++VYEVV+LVLENYRLVQREI VR
Sbjct: 181 KQGKISELDLWGELDVLVGFVEGICEFPDSLHLQKNEMVYEVVRLVLENYRLVQREISVR 240

Query: 241 VKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAET 300
           V+ IGDR DSLSLDELT+LV IL R ENCRR L+VLFVNR KNE+ WELVK TK KL E 
Sbjct: 241 VRGIGDRADSLSLDELTQLVVILTRFENCRRKLTVLFVNRAKNEDLWELVKNTKAKLVEQ 300

Query: 301 KGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
           K MKEEKRMIMVE+RA+SVE TR WNPFVEPGQLLWVP+GD  LGPA LPLTVSTVG
Sbjct: 301 KQMKEEKRMIMVEIRAESVELTRLWNPFVEPGQLLWVPSGDEPLGPALLPLTVSTVG 355

[Close]

BLAST of Lsi08G009920 vs. NCBI nr
Match: KAG6598708.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.2 bits (1232), Expect = 3.2e-131
Identity = 265/358 (74.02%), Postives = 287/358 (80.17%), Query Frame = 0


Query: 1   MAIDQNKKLKNLIHTLKDKASIIKATFSIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAV 60
           MAIDQ KK KNLI   KD+ASIIKATFSIHRRSSSIKVAVVRATTHG+RNPPSD R+AA+
Sbjct: 1   MAIDQTKKFKNLIDAFKDQASIIKATFSIHRRSSSIKVAVVRATTHGARNPPSDARLAAL 60

Query: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCY 120
           LA GNDFRSSTAF CI+ALMERLHTT+SAAVA+KSLFTLHII IRGPFNLR +VAF P Y
Sbjct: 61  LAFGNDFRSSTAFLCIQALMERLHTTTSAAVAMKSLFTLHIIAIRGPFNLRGEVAFSPYY 120

Query: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDH 180
           GGRNFLNLSAFRDVSDSEM++LS WVRWYAGVVEHN    RKLDRILYFRSRN EIV   
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSELSCWVRWYAGVVEHN----RKLDRILYFRSRNPEIV--- 180

Query: 181 KEGRKRKI-DLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWV 240
            EG+ RKI +L EEL+VLV F ERI EVPESLH+QK DLVYEVV+LVLE+YRLVQREIWV
Sbjct: 181 -EGKDRKIPELLEELDVLVGFSERISEVPESLHVQKSDLVYEVVRLVLESYRLVQREIWV 240

Query: 241 RVKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKNEEFWELVKITKGKLAE 300
           RV EIG+RV+ +S DELTE V IL R+ENCRR +SVLFVNRGKNEE WELV  TKGKL E
Sbjct: 241 RVNEIGNRVEWVSRDELTESVEILTRMENCRRKVSVLFVNRGKNEELWELVTCTKGKLVE 300

Query: 301 TKGMKEEKRMIMVEMRADSVESTRFWNPFVEPGQLLWVPAGDGHLGPAPLPLTVSTVG 358
            +            M     ESTR WNPFVEPG L          GPA LPLTVSTVG
Sbjct: 301 RRR----------RMTTTMTESTRLWNPFVEPGSL-------RPFGPAFLPLTVSTVG 333

[Close]

BLAST of Lsi08G009920 vs. TAIR 10
Match: AT4G40080.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 5.3e-44
Identity = 123/328 (37.50%), Postives = 179/328 (54.57%), Query Frame = 0


Query: 11  NLIHTLKDKASIIKATF---SIHRRSSSIKVAVVRATTHGSRNPPSDGRVAAVLALGNDF 70
           +LI  +KDKAS  KA     +   ++ S  ++V+RATTH    PP +  +A +L+ G   
Sbjct: 9   DLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAVILSAGTGS 68

Query: 71  RSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGGRNFLN 130
           R +TA + +E++MERLHTT  A VA+KSL  +H IV  G F L+DQ++ FP  GGRN+L 
Sbjct: 69  R-ATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPASGGRNYLK 128

Query: 131 LSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDHKEGRKRK 190
           LSAFRD     M +LSSWVRWYA  +EH +   R +    +F S     +  HKE  +  
Sbjct: 129 LSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMG---FFISSTSSTI--HKEEYEEM 188

Query: 191 I------DLSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVRV 250
           +      DL  E++ LV  +E  C++P+      K L  ++ +LV E+Y     E++ R 
Sbjct: 189 VSSLTNSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYTRF 248

Query: 251 KEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLF---VNRGKNEEFWELVKITKGKLA 310
            E  +R ++LS  +  ELV  L+RLE+C+  LS +      RG  + FW LV   KG + 
Sbjct: 249 NEFKERSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWGLVLEVKGIIG 308

Query: 311 --ETKGMKEEKRMIMVEMRADSVESTRF 325
             E    + EK ++    R    ES RF
Sbjct: 309 NLEDNYGQIEKSIVGFGKRDKGYESARF 330

[Close]

BLAST of Lsi08G009920 vs. TAIR 10
Match: AT5G10410.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 5.4e-20
Identity = 97/313 (30.99%), Postives = 158/313 (50.48%), Query Frame = 0


Query: 11  NLIHTLKDKASIIKATFSIHRRSSSIK---VAVVRATTHGSRNPPSDGRVAAVLALGNDF 70
           ++I   KDKASI KA       S+++K   +A++++TT     PP+   V+AV++  N  
Sbjct: 7   HIIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISYSNSR 66

Query: 71  RSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGGRNFLN 130
            +  AF+   A + RL  T +A VA KSL  +H ++     + RD+  F     GRN L 
Sbjct: 67  YAPAAFS---AALWRLRVTKNAIVATKSLIVIHKLI----KSSRDK--FEGLGHGRNNLK 126

Query: 131 LSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEIVEDHKEGRKRK 190
           L+ F D S +   +LS W+RWY   ++    V + L           + VE+       +
Sbjct: 127 LNEFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQ 186

Query: 191 ID-LSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLENYRLVQREIWVRVKEIGD 250
              +  + + LVSF E IC  PE   + +  +V E+ +LV+E+Y  + R + VR++ + +
Sbjct: 187 TGYIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFE 246

Query: 251 R--------VDSLSLDELTELVGILRRLENCRRNLSVLFVN-RGKNEEFWELVKITKGKL 310
           R        +  L L++ + L   L RL  C+ +LS LF   R   ++FW LV++ K   
Sbjct: 247 RLIKPGVKPIGDLGLNDFSLL---LVRLVECKESLSGLFWRCRRLADDFWCLVEMLK--- 303

[Close]

BLAST of Lsi08G009920 vs. TAIR 10
Match: AT5G65370.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 1.4e-15
Identity = 88/307 (28.66%), Postives = 145/307 (47.23%), Query Frame = 0


Query: 8   KLKNLIHTLKDKASIIKATFSIHRRSS----SIKVAVVRATTHGSRNPPSDGRVAAVLAL 67
           KL  L   LKD+AS +K    +H  SS    +I +A+++AT+H S NPPSD  V      
Sbjct: 3   KLATLNGILKDEASQMKLNV-VHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVT----- 62

Query: 68  GNDFRSSTAFAC-----IEALMERLHTTSSAAVAIKSLFTLHIIV-----IRGPFNLRDQ 127
              F  ST   C     ++A++ RL  T+   VA K L  LH +V       G  +LR+ 
Sbjct: 63  ---FLQSTIDTCYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNN 122

Query: 128 V---AFFPCYGGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFR 187
           +         GG N L L+     S     +L+ WV+WY   ++  + +   L      +
Sbjct: 123 INHRTLIYTQGGSN-LKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIK 182

Query: 188 SRNCEIVEDHKEGRKRKID-LSEELEVLVSFVERICEVPESLHLQKKDLVYEVVKLVLEN 247
            +N +   + +      +D + ++++ LV   E I + P++   +   +V E+ +L++++
Sbjct: 183 EKNEDKRLETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELMVQD 242

Query: 248 YRLVQREIWVRVKEIGDRVDSLSLDELTELVGILRRLENCRRNLSVLFVNRGKN--EEFW 295
           Y    R + +R +E+  RV      +  ELV +L +LENC+  LS  F  R K    +FW
Sbjct: 243 YFSAIRLMRIRFEELNVRV-----AKPNELVPVLEKLENCKEGLSE-FSWRSKYLIADFW 293

[Close]

BLAST of Lsi08G009920 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 5.9e-11
Identity = 55/162 (33.95%), Postives = 86/162 (53.09%), Query Frame = 0


Query: 6   NKKLKNLIHTLKDKASIIKATFSIHRRSSS---IKVAVVRATTHGSRNPPSDGRVAAVLA 65
           + KLK  I  +KD+ S+  A   +  RSSS   +++AVV+AT H    P  D  +  +L 
Sbjct: 3   SSKLKRAIGAVKDQTSVGLA--KVGGRSSSLTELEIAVVKATRHDD-YPAEDKYIREILC 62

Query: 66  LGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGG 125
           L +  R+  + AC+  L  RL+ T + +VA+K+L  +  ++  G     +Q  FF    G
Sbjct: 63  LTSYSRNYVS-ACVATLSRRLNKTKNWSVALKTLILIQRLLTDGD-RAYEQEIFFATRRG 122

Query: 126 RNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLD 165
              LN+S FRD S S+  D S++VR YA      + +D +LD
Sbjct: 123 TRLLNMSDFRDASQSDSWDYSAFVRTYA------LYLDERLD 153

[Close]

BLAST of Lsi08G009920 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-09
Identity = 53/162 (32.72%), Postives = 82/162 (50.62%), Query Frame = 0


Query: 6   NKKLKNLIHTLKDKASIIKATFSIHRRSSSIK---VAVVRATTHGSRNPPSDGRVAAVLA 65
           + K K  I  +KD+ S+  A   ++ RS+S+    VA+V+AT H    P  +  +  +L+
Sbjct: 3   SSKFKRAIGAVKDQTSVGLA--KVNGRSASLSELDVAIVKATRH-EEFPAEEKYIREILS 62

Query: 66  LGNDFRSSTAFACIEALMERLHTTSSAAVAIKSLFTLHIIVIRGPFNLRDQVAFFPCYGG 125
           L   +  S   AC+  L  RL+ T    VA+K+L  +  ++  G     +Q  FF    G
Sbjct: 63  L-TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGD-QAYEQEIFFATRRG 122

Query: 126 RNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLD 165
              LN+S FRDVS S   D S++VR YA      + +D +LD
Sbjct: 123 TRLLNMSDFRDVSRSNSWDYSAFVRTYA------LYLDERLD 153

The following BLAST results are available for this feature:

BLAST of Lsi08G009920 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L936	7.5e-43	37.50	Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8H0W9	7.5e-19	30.99	Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9FKQ2	1.9e-14	28.66	Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8GX47	8.4e-10	33.95	Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q9SA65	1.6e-08	32.72	Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Lsi08G009920 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VCW1	5.6e-166	86.83	Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A1S3BDW7	5.6e-166	86.83	putative clathrin assembly protein At4g40080 OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A0A0LLA1	8.6e-159	84.31	ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369220 PE=4 S...	[more]
A0A6J1BR25	1.2e-147	78.15	putative clathrin assembly protein At4g40080 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1HF54	2.0e-131	74.58	putative clathrin assembly protein At4g40080 OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of Lsi08G009920 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884022.1	4.6e-175	91.32	putative clathrin assembly protein At4g40080 [Benincasa hispida]	[more]
XP_008445571.1	1.1e-165	86.83	PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis melo] >KAA00651...	[more]
XP_004152749.1	1.8e-158	84.31	putative clathrin assembly protein At4g40080 [Cucumis sativus] >KGN62720.1 hypot...	[more]
XP_022131457.1	2.4e-147	78.15	putative clathrin assembly protein At4g40080 [Momordica charantia]	[more]
KAG6598708.1	3.2e-131	74.02	putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Lsi08G009920 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G40080.1	5.3e-44	37.50	ENTH/ANTH/VHS superfamily protein	[more]
AT5G10410.1	5.4e-20	30.99	ENTH/ANTH/VHS superfamily protein	[more]
AT5G65370.1	1.4e-15	28.66	ENTH/ANTH/VHS superfamily protein	[more]
AT4G02650.1	5.9e-11	33.95	ENTH/ANTH/VHS superfamily protein	[more]
AT1G03050.1	1.1e-09	32.72	ENTH/ANTH/VHS superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013809	ENTH domain	SMART	SM00273	enth_2	coord: 34..165 e-value: 0.0037 score: 12.4
IPR013809	ENTH domain	PROSITE	PS50942	ENTH	coord: 28..167 score: 12.004757
IPR011417	AP180 N-terminal homology (ANTH) domain	PFAM	PF07651	ANTH	coord: 36..229 e-value: 4.2E-13 score: 48.9
IPR008942	ENTH/VHS	GENE3D	1.25.40.90		coord: 7..164 e-value: 1.0E-24 score: 88.8
IPR008942	ENTH/VHS	SUPERFAMILY	48464	ENTH/VHS domain	coord: 34..163
None	No IPR available	PANTHER	PTHR22951	CLATHRIN ASSEMBLY PROTEIN	coord: 4..356
None	No IPR available	PANTHER	PTHR22951:SF24	CLATHRIN ASSEMBLY PROTEIN	coord: 4..356
None	No IPR available	CDD	cd16987	ANTH_N_AP180_plant	coord: 36..159 e-value: 1.48316E-46 score: 152.778

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G009920.1	Lsi08G009920.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0072583	clathrin-dependent endocytosis
biological_process	GO:0006900	vesicle budding from membrane
cellular_component	GO:0005905	clathrin-coated pit
cellular_component	GO:0030136	clathrin-coated vesicle
cellular_component	GO:0005794	Golgi apparatus
molecular_function	GO:0005545	1-phosphatidylinositol binding
molecular_function	GO:0032050	clathrin heavy chain binding
molecular_function	GO:0005546	phosphatidylinositol-4,5-bisphosphate binding
molecular_function	GO:0000149	SNARE binding
molecular_function	GO:0005543	phospholipid binding