Lsi08G009650 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G009650
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAB hydrolase-1 domain-containing protein
Locationchr08: 18144523 .. 18146532 (+)
RNA-Seq ExpressionLsi08G009650
SyntenyLsi08G009650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGAACAGAGTGGTTTCAAGCTCAGTGTCTAATCCGAGTTATGGAGGCTAACTCAGTGGCGAAACTCAGTTTGGTGGGGCTTAGCTATGGAGGGTTTGTTGGGTACAGCATAGCGGCGCTGCGGCCTGAAATGGTGGAGAAAGTAGTGATTTGTTGCTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGACGGGTTGTTGAAAGTCTCTGATTTGGAAGAAGCTACAGCCATTTTGGTGCCCCAAAAGCCAGAGAAGCTTAAACAGTTGGTGGGTTACTCGTTTTTCCGCCCGCCGCCGATGCGGTTGATGCCCTCTTGTCTGCTCAATGATTTCATTGAGGTATGGATGGAGGATTCAAACTTCTAACTTGTTAGTCGAGAATATATACCTAAACTAAGTTTTCCAACTTTTATATCTACACTACTTGCATTTCATACTTAACAATTTTTTACTACACACAATTCGACCTATAAACAAGGTTATGTTTGGTATGTGAATCTATTCATTCTTTTGTTAATTCCAGTGCACTATTAAGTTCATGACATCTACATTTGATATTACATATTAACTTCTTCAAATTATTAAGTTGCACAAATCCAGGTTGAATACAAACCCAAACATAAGCTAGTTTTATAAGTAACAAACTCATTTTGTGTTAATTTGACTAAACTTTGGGGTATGTGGTAGTCAATGTGTTTGGACCATATTGAAGAAAAGAGGGAGCTGATTCGAACTATTCCCAGAGGGAGAAAGCTTTCAGACCTTCCCAAGATTCACCAGGTTGGATTGCTATTCAATACAACTTATCCGCATTAGCTTTAAACAGTTCATTAACTACTTATCTTCATTGGCATTTCATGATTCTGTTTGATTGTTTGTTTTAAGTGAAATTGGGAAGGTTCAAAAAGTTGAGTCGTTTGTATACGGAAATAGCTCTCGAGCTTTAAAATTTGTATCAATTTGGTTCATAAACTTTCAAAAGTGTTAATAGTTCCACAATAAGTAACTTTAAAAATTGATCAACAGGCCCTTAAAATTCCAATTTCATGTCCAATTCTAAAATAGGTCTTCGAACTCTTTTTATTGTTTTTTTTTTACATACAACTGAAAGGTCCAAGGATTTATGAAACACAAATTAAAAAATTTATGAGCTATTAGACATCTCTAATTGCCACTTTTAAAAGTTTTCAAAAAATTTATGAGCTAACAGTGAGATATTAGACATCTCTAATTGCCACTTTTAAAAGTTTTCAAAGACTATACTTATAATTTAAACATTTGTCAACTTGCTCGCAGCGGACGATGATACTGTGGGGGGAACATGACCAAGTATTTCCAGTAGAATTGGGTTACAGATTGAAAAGGTGAGTAATACAACTCCTTAAAAGCAAACAATCGATCAAATATTCTCATTTAGAGGCAAATGATAAATGTGTTGGCCACTCCTTTGAAGTAGGCATTTGGGGGACAATGCTACGCTTGTAGTAATCAAGAATGCAGGCCATGCCTTCAACTCAGAGGAGCCAAAGGAGTTTCTCAACCATTTGATTTCTTTCCTGGTTGATTCATAGCCTCCCCCCATGACGTTTCGGCAGTTCAAATTCACAAACGACAACGTCAAGAAAAAGAAAATGAACAACACCAAAAGTTGGAGTGATCTAACGATTGGTTTTAGTACAACAAACAAGAGAAGAAACATACCTTGACCACATTAAACAATTAAATTTCTTATAGGAATTTTTTACATTTGCCATTTTGCCTCTTAATTCCAAGGACTTAAAACTCAGAAATCTTCAACCATAATATTTGTAATCCCAAAAATGTCCCTCAACTATCAGATTCCATGTGTAACTCTCTTGTACTATTCTACACCAGTGCTCTCTGTGCCATTGTTTACATCAAGTCTATTTTAATGTCTGGCAACAATTCCATCCGATGACTAATGGGACCAAAAAATAAAGCATAATATGAAATAGTAAAACCATGAATGAGCCTATTCTACTCC

mRNA sequence

ACAGAACAGAGTGGTTTCAAGCTCAGTGTCTAATCCGAGTTATGGAGGCTAACTCAGTGGCGAAACTCAGTTTGGTGGGGCTTAGCTATGGAGGGTTTGTTGGGTACAGCATAGCGGCGCTGCGGCCTGAAATGGTGGAGAAAGTAGTGATTTGTTGCTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGACGGGTTGTTGAAAGTCTCTGATTTGGAAGAAGCTACAGCCATTTTGGTGCCCCAAAAGCCAGAGAAGCTTAAACAGTTGGTGGAAAAGAGGGAGCTGATTCGAACTATTCCCAGAGGGAGAAAGCTTTCAGACCTTCCCAAGATTCACCAGCGGACGATGATACTGTGGGGGGAACATGACCAAGTATTTCCAGTAGAATTGGGTTACAGATTGAAAAGTAGGCATTTGGGGGACAATGCTACGCTTGTAGTAATCAAGAATGCAGGCCATGCCTTCAACTCAGAGGAGCCAAAGGAGTTTCTCAACCATTTGATTTCTTTCCTGGTTGATTCATAGCCTCCCCCCATGACGTTTCGGCAGTTCAAATTCACAAACGACAACGTCAAGAAAAAGAAAATGAACAACACCAAAAGTTGGAGTGATCTAACGATTGGTTTTAGTACAACAAACAAGAGAAGAAACATACCTTGACCACATTAAACAATTAAATTTCTTATAGGAATTTTTTACATTTGCCATTTTGCCTCTTAATTCCAAGGACTTAAAACTCAGAAATCTTCAACCATAATATTTGTAATCCCAAAAATGTCCCTCAACTATCAGATTCCATGTGTAACTCTCTTGTACTATTCTACACCAGTGCTCTCTGTGCCATTGTTTACATCAAGTCTATTTTAATGTCTGGCAACAATTCCATCCGATGACTAATGGGACCAAAAAATAAAGCATAATATGAAATAGTAAAACCATGAATGAGCCTATTCTACTCC

Coding sequence (CDS)

ATGGAGGCTAACTCAGTGGCGAAACTCAGTTTGGTGGGGCTTAGCTATGGAGGGTTTGTTGGGTACAGCATAGCGGCGCTGCGGCCTGAAATGGTGGAGAAAGTAGTGATTTGTTGCTCTGGGGTTTGTGTGGAAGAGAAGGATTTTAAAGACGGGTTGTTGAAAGTCTCTGATTTGGAAGAAGCTACAGCCATTTTGGTGCCCCAAAAGCCAGAGAAGCTTAAACAGTTGGTGGAAAAGAGGGAGCTGATTCGAACTATTCCCAGAGGGAGAAAGCTTTCAGACCTTCCCAAGATTCACCAGCGGACGATGATACTGTGGGGGGAACATGACCAAGTATTTCCAGTAGAATTGGGTTACAGATTGAAAAGTAGGCATTTGGGGGACAATGCTACGCTTGTAGTAATCAAGAATGCAGGCCATGCCTTCAACTCAGAGGAGCCAAAGGAGTTTCTCAACCATTTGATTTCTTTCCTGGTTGATTCATAG

Protein sequence

MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVPQKPEKLKQLVEKRELIRTIPRGRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPKEFLNHLISFLVDS
Homology
BLAST of Lsi08G009650 vs. ExPASy Swiss-Prot
Match: O05235 (Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yugF PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 5.7e-06
Identity = 45/189 (23.81%), Postives = 81/189 (42.86%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSG------------------- 60
           +E   V +  LVG S GG +  S A  +PE+  KVV+ CS                    
Sbjct: 88  LEHLQVKQAVLVGHSMGGQISLSAALQKPELFSKVVLLCSSGYLKRSHPTIIFGTHIPYF 147

Query: 61  -VCVEEKDFKDGLLK----------VSDLEEATAILVPQKPEKLKQLVEKRELIRTIPRG 120
            + ++    K+G++K          + D E       P + E++ + + +   IR     
Sbjct: 148 HLYIKRWLSKEGVMKNLLNVVHDKSLIDEEMIDGYGRPFQDEQIFKAMTR--FIRHREGD 207

Query: 121 RKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPKE 160
            +   L K+++  +++WGE D++ P+E+G RL +     N+ L  +   GH    E P+ 
Sbjct: 208 LEPEQLKKMNKPALLIWGEEDRIVPMEIGKRLHADL--PNSVLYSLGQTGHLVPEERPEL 267

BLAST of Lsi08G009650 vs. ExPASy TrEMBL
Match: A0A5A7VI51 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2, 4-dienoic acid hydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004850 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 7.3e-73
Identity = 150/192 (78.12%), Postives = 156/192 (81.25%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEA++V K SLVGLSYGGFVGYSIAALRPEMVE+VVICCSGVCVEEKDFKDGLLKVSDLE
Sbjct: 112 MEAHTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           EATAILVPQKPEKLKQLV                               EKRELIRTIPR
Sbjct: 172 EATAILVPQKPEKLKQLVGYSFFRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 162
           GRKLSDLPKI QRT+I+WGEHDQVFPVELG+RLK RHLGDNATLVVIKN GHAFNSEEPK
Sbjct: 232 GRKLSDLPKIQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. ExPASy TrEMBL
Match: A0A1S3BC20 (LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase OS=Cucumis melo OX=3656 GN=LOC103488063 PE=4 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 7.3e-73
Identity = 150/192 (78.12%), Postives = 156/192 (81.25%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEA++V K SLVGLSYGGFVGYSIAALRPEMVE+VVICCSGVCVEEKDFKDGLLKVSDLE
Sbjct: 112 MEAHTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           EATAILVPQKPEKLKQLV                               EKRELIRTIPR
Sbjct: 172 EATAILVPQKPEKLKQLVGYSFXRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 162
           GRKLSDLPKI QRT+I+WGEHDQVFPVELG+RLK RHLGDNATLVVIKN GHAFNSEEPK
Sbjct: 232 GRKLSDLPKIQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. ExPASy TrEMBL
Match: A0A0A0LLN7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368940 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.4e-71
Identity = 148/192 (77.08%), Postives = 155/192 (80.73%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEAN+V K SLVGLSYGGFVGYSIAALRPEMVE+VVICCSGVCVEEKDFKDGLLKVS LE
Sbjct: 112 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           +ATAILVPQKPEKLKQLV                               EKRELIRTIPR
Sbjct: 172 DATAILVPQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 162
           GRKLSDLPKI QRTMI+WGEHDQVFP+ELG+RLK RHLGDNATLVVIKN GHAFNSEEPK
Sbjct: 232 GRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. ExPASy TrEMBL
Match: A0A6J1HDR2 (uncharacterized protein LOC111462585 OS=Cucurbita moschata OX=3662 GN=LOC111462585 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.1e-71
Identity = 145/193 (75.13%), Postives = 157/193 (81.35%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEANSV KLSLVGLSYGGFVGYS+AAL P+MVEKVVICC+GVCVEEKDFKDGLL+VSDLE
Sbjct: 112 MEANSVGKLSLVGLSYGGFVGYSMAALCPDMVEKVVICCAGVCVEEKDFKDGLLRVSDLE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           EA AILVPQKPEKLKQLV                               EKRELIRTIP+
Sbjct: 172 EAIAILVPQKPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPK 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 163
           GRKLSDLPKIHQRTMILWGEHD+VFP+ELG+RLK RHLGDNATLV++KNAGHAFNSEEPK
Sbjct: 232 GRKLSDLPKIHQRTMILWGEHDEVFPLELGHRLK-RHLGDNATLVIVKNAGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. ExPASy TrEMBL
Match: A0A6J1KAV5 (uncharacterized protein LOC111492191 OS=Cucurbita maxima OX=3661 GN=LOC111492191 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.5e-70
Identity = 144/193 (74.61%), Postives = 156/193 (80.83%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEANSV KLSLVGLSYGGFVGYS+AAL P+MVEKVVICC+G+CVEEKDFKDGLL+VSDLE
Sbjct: 112 MEANSVGKLSLVGLSYGGFVGYSMAALCPDMVEKVVICCAGICVEEKDFKDGLLRVSDLE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           EA AILVPQKPEKLKQLV                               EKRELIRTIP+
Sbjct: 172 EAIAILVPQKPEKLKQLVGYSFFRPPPMRLMPSCLLNDFIESMCLDHIDEKRELIRTIPK 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 163
           GRKLSDLPKIH RTMILWGEHD++FP+ELG+RLK RHLGDNATLV+IKNAGHAFNSEEPK
Sbjct: 232 GRKLSDLPKIHPRTMILWGEHDEIFPLELGHRLK-RHLGDNATLVIIKNAGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. NCBI nr
Match: XP_038885546.1 (uncharacterized protein LOC120075885 [Benincasa hispida])

HSP 1 Score: 290.0 bits (741), Expect = 1.2e-74
Identity = 153/193 (79.27%), Postives = 159/193 (82.38%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEANSV KLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSG+CVEEKDFKDGLLKVSDLE
Sbjct: 112 MEANSVVKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGICVEEKDFKDGLLKVSDLE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           EATAILVPQKP+KLKQLV                               EKRELIRTIPR
Sbjct: 172 EATAILVPQKPDKLKQLVGYSFFRPPPLRLMPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 163
           GRKLSDLPKIHQRTMI+WGEHDQVFP+ELG+RLK RHLG NATLVV+KNAGHAFNSEEPK
Sbjct: 232 GRKLSDLPKIHQRTMIMWGEHDQVFPLELGHRLK-RHLGANATLVVVKNAGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. NCBI nr
Match: XP_031737138.1 (uncharacterized protein LOC101222914 isoform X1 [Cucumis sativus])

HSP 1 Score: 285.0 bits (728), Expect = 4.0e-73
Identity = 149/192 (77.60%), Postives = 156/192 (81.25%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEAN+V K SLVGLSYGGFVGYSIAALRPEMVE+VVICCSGVCVEEKDFKDGLLKVS LE
Sbjct: 112 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           +ATAILVPQKPEKLKQLV                               EKRELIRTIPR
Sbjct: 172 DATAILVPQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 162
           GRKLSDLPKI QRTMI+WGEHDQVFP+ELG+RLKSRHLGDNATLVVIKN GHAFNSEEPK
Sbjct: 232 GRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLKSRHLGDNATLVVIKNTGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. NCBI nr
Match: XP_008444836.2 (PREDICTED: LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 150/192 (78.12%), Postives = 156/192 (81.25%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEA++V K SLVGLSYGGFVGYSIAALRPEMVE+VVICCSGVCVEEKDFKDGLLKVSDLE
Sbjct: 112 MEAHTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           EATAILVPQKPEKLKQLV                               EKRELIRTIPR
Sbjct: 172 EATAILVPQKPEKLKQLVGYSFXRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 162
           GRKLSDLPKI QRT+I+WGEHDQVFPVELG+RLK RHLGDNATLVVIKN GHAFNSEEPK
Sbjct: 232 GRKLSDLPKIQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. NCBI nr
Match: KAA0065149.1 (2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo var. makuwa])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 150/192 (78.12%), Postives = 156/192 (81.25%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEA++V K SLVGLSYGGFVGYSIAALRPEMVE+VVICCSGVCVEEKDFKDGLLKVSDLE
Sbjct: 112 MEAHTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSDLE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           EATAILVPQKPEKLKQLV                               EKRELIRTIPR
Sbjct: 172 EATAILVPQKPEKLKQLVGYSFFRPPPLRLLPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 162
           GRKLSDLPKI QRT+I+WGEHDQVFPVELG+RLK RHLGDNATLVVIKN GHAFNSEEPK
Sbjct: 232 GRKLSDLPKIQQRTLIMWGEHDQVFPVELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. NCBI nr
Match: XP_004152639.1 (uncharacterized protein LOC101222914 isoform X2 [Cucumis sativus] >KGN62693.1 hypothetical protein Csa_022618 [Cucumis sativus])

HSP 1 Score: 278.9 bits (712), Expect = 2.8e-71
Identity = 148/192 (77.08%), Postives = 155/192 (80.73%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEAN+V K SLVGLSYGGFVGYSIAALRPEMVE+VVICCSGVCVEEKDFKDGLLKVS LE
Sbjct: 112 MEANTVGKFSLVGLSYGGFVGYSIAALRPEMVERVVICCSGVCVEEKDFKDGLLKVSALE 171

Query: 61  EATAILVPQKPEKLKQLV-------------------------------EKRELIRTIPR 120
           +ATAILVPQKPEKLKQLV                               EKRELIRTIPR
Sbjct: 172 DATAILVPQKPEKLKQLVGYSFFRPPPLRLIPSCLLNDFIESMCLDHIEEKRELIRTIPR 231

Query: 121 GRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPK 162
           GRKLSDLPKI QRTMI+WGEHDQVFP+ELG+RLK RHLGDNATLVVIKN GHAFNSEEPK
Sbjct: 232 GRKLSDLPKIQQRTMIMWGEHDQVFPLELGHRLK-RHLGDNATLVVIKNTGHAFNSEEPK 291

BLAST of Lsi08G009650 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 210.7 bits (535), Expect = 8.8e-55
Identity = 105/191 (54.97%), Postives = 140/191 (73.30%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           MEA+ V ++++VG+SYGGFVGYS+AA  PE VEK+V+CC+GVC+EEKD +DGL KV +LE
Sbjct: 121 MEAHGVQRMNIVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLE 180

Query: 61  EATAILVPQKPEKLKQLV-----------------------------EKRELIRTIPRGR 120
           EAT IL+PQ PEKLK+L+                             EKR+LI++I + R
Sbjct: 181 EATGILIPQTPEKLKELIRFSFVKPIKGVPSFFLWDFIDVMCTEFVEEKRDLIKSILKDR 240

Query: 121 KLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPKEF 163
           +LSDLP+I Q+++I+WGE DQ+FP+ELGYRLK RH+G++A +VVIK AGHA N E+ KEF
Sbjct: 241 RLSDLPRIKQKSLIIWGEEDQIFPLELGYRLK-RHIGESAEIVVIKKAGHAVNLEKSKEF 300

BLAST of Lsi08G009650 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 190.7 bits (483), Expect = 9.5e-49
Identity = 98/194 (50.52%), Postives = 132/194 (68.04%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           +EA SV K SLVGLSYGGFVGY +A++  + VEKVVICC+ VCVEEKD K G+ KVSDL+
Sbjct: 112 LEAQSVKKFSLVGLSYGGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLD 171

Query: 61  EATAILVPQKPEKLKQLV--------------------------------EKRELIRTIP 120
           EA+ ILVP+  +KL++L+                                EKRELI+ IP
Sbjct: 172 EASKILVPESVKKLRELMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAIP 231

Query: 121 RGRKLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEP 163
           + R +S++PK+ Q T+I+WGEHDQVFP+E+G RL+ +H+GDN  LV+IK  GH FN E+P
Sbjct: 232 KDRIISEIPKLKQPTLIIWGEHDQVFPLEMGKRLE-KHVGDNGKLVIIKRTGHIFNFEKP 291

BLAST of Lsi08G009650 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 2.3e-39
Identity = 85/189 (44.97%), Postives = 118/189 (62.43%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLE 60
           M+A  V  +++ GLSYGGFV YS+AA   E V++VV+ C+GV +EEKD +DG+ KV   E
Sbjct: 110 MDAYGVRTMTVAGLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPE 169

Query: 61  EATAILVPQKPEKLKQLV-----------------------------EKRELIRTIPRGR 120
           EA A+L PQ P  L++L+                             E++EL+  + +GR
Sbjct: 170 EAAAVLFPQSPSMLRRLLQLSFYKPPIWIPSCFAMDYIHVMCKDYLQERKELVEALHKGR 229

Query: 121 KLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLG-DNATLVVIKNAGHAFNSEEPKE 160
           + ++LPKI Q T+++WGE DQVFPVEL +RLK R+LG D A LV++K  GHA N E+PKE
Sbjct: 230 RFANLPKITQPTLMIWGEEDQVFPVELAHRLK-RYLGEDRAQLVLLKKTGHAINEEKPKE 289

BLAST of Lsi08G009650 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 92.4 bits (228), Expect = 3.5e-19
Identity = 64/189 (33.86%), Postives = 98/189 (51.85%), Query Frame = 0

Query: 1   MEANSVAKLSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKD------------ 60
           ME   V + S+VG SYGGFV Y++A + PE VEKVV+  SGV +   D            
Sbjct: 115 MEKLEVERFSVVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHR 174

Query: 61  FKDGLLKVS--DLEEATAILVPQKPEKLKQLV--------------EKRELIRTIPRGR- 120
            K+ +L  S  DL   + ++  ++ + +   V              EK EL+  +  G+ 
Sbjct: 175 IKEVMLPASATDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKD 234

Query: 121 KLSDLPKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPKEF 161
             +++  I Q  M++WGE DQVFP+++ + LK   LG  ATL VI+   H   +E+ KEF
Sbjct: 235 DKTNVSPIQQDVMLIWGEQDQVFPLKMAHDLKEM-LGTKATLKVIQKTSHIPQTEKSKEF 294

BLAST of Lsi08G009650 vs. TAIR 10
Match: AT1G17430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 7.8e-19
Identity = 62/186 (33.33%), Postives = 93/186 (50.00%), Query Frame = 0

Query: 9   LSLVGLSYGGFVGYSIAALRPEMVEKVVICCSGVCVEEKDFKDGLLKVSDLEEATAILVP 68
           +S+  +SYGGFV Y +A + P MVEK+VI  SGV   ++     L K     + + ILVP
Sbjct: 151 ISIYSISYGGFVAYKMAEIWPAMVEKLVIVSSGVGFTQQQKTAELKKHGG--DCSKILVP 210

Query: 69  QKPEKLKQLV--------------------------------EKRELIRTIPRGRKLSDL 128
           + P  L+ L+                                E  EL + +    +  +L
Sbjct: 211 KTPMDLRLLIKISMNTGLTFVDWVPDFFLSQFIAVMYEKNRQELLELAKNLLEREEEPEL 270

Query: 129 PKIHQRTMILWGEHDQVFPVELGYRLKSRHLGDNATLVVIKNAGHAFNSEEPKEFLNHLI 163
           P I Q+T+I+WG+ D+VFP+E  YRL+ RHL  ++ L +IK  GHA N E P    N + 
Sbjct: 271 PVISQKTLIVWGDKDKVFPLEHAYRLQ-RHL-QSSRLEIIKETGHAVNIEAPTTLNNFIT 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O052355.7e-0623.81Uncharacterized hydrolase YugF OS=Bacillus subtilis (strain 168) OX=224308 GN=yu... [more]
Match NameE-valueIdentityDescription
A0A5A7VI517.3e-7378.132-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2, 4-dienoic acid hydrolase OS=Cucumis me... [more]
A0A1S3BC207.3e-7378.13LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hyd... [more]
A0A0A0LLN71.4e-7177.08AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368... [more]
A0A6J1HDR23.1e-7175.13uncharacterized protein LOC111462585 OS=Cucurbita moschata OX=3662 GN=LOC1114625... [more]
A0A6J1KAV51.5e-7074.61uncharacterized protein LOC111492191 OS=Cucurbita maxima OX=3661 GN=LOC111492191... [more]
Match NameE-valueIdentityDescription
XP_038885546.11.2e-7479.27uncharacterized protein LOC120075885 [Benincasa hispida][more]
XP_031737138.14.0e-7377.60uncharacterized protein LOC101222914 isoform X1 [Cucumis sativus][more]
XP_008444836.21.5e-7278.13PREDICTED: LOW QUALITY PROTEIN: 2-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dieno... [more]
KAA0065149.11.5e-7278.132-hydroxy-6-oxo-6-(2'-aminophenyl)hexa-2,4-dienoic acid hydrolase [Cucumis melo ... [more]
XP_004152639.12.8e-7177.08uncharacterized protein LOC101222914 isoform X2 [Cucumis sativus] >KGN62693.1 hy... [more]
Match NameE-valueIdentityDescription
AT5G09430.18.8e-5554.97alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.19.5e-4950.52alpha/beta-Hydrolases superfamily protein [more]
AT4G39955.12.3e-3944.97alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.13.5e-1933.86alpha/beta-Hydrolases superfamily protein [more]
AT1G17430.17.8e-1933.33alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..161
e-value: 1.0E-25
score: 92.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..159
IPR014940BAAT/Acyl-CoA thioester hydrolase C-terminalPFAMPF08840BAAT_Ccoord: 8..144
e-value: 3.7E-6
score: 27.1
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 79..161
coord: 1..78
NoneNo IPR availablePANTHERPTHR43139:SF54ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 1..78
NoneNo IPR availablePANTHERPTHR43139:SF54ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 79..161

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G009650.1Lsi08G009650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity