Lsi08G009220 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G009220
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionU-box domain-containing protein 11-like
Locationchr08: 17626080 .. 17627036 (+)
RNA-Seq ExpressionLsi08G009220
SyntenyLsi08G009220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTAAAGCGCCGTACAGCTTCCACTTTAGTCTCTCGCCTCAGCTCCGTCTCCGAGCAAATCCGGTGCGAATCCTTGTCGGAGCTCCGATTGATGACCAAGAACGACGCCGAAAGCCGATCACTTATCGTCGAAGCCGGTGCCATTCCTTACTTGGCTGAAACTCTGTATTCCTCTTCTCACGCCGCCCAAGAAGACGCCGCCGCTACGCTTCTCAACCTCTCAATCTCCTCCCGTGAAGCTTTAATGTCCACTCGCGGCCTCCTCGACGCGATTTCTCATGCACTTAGCCATCACAATTCCTCTTCCTCCGCCTCCGCCGTCCAATCCTGTGCTGCGACTCTCCATAGTCTTCTCGTTGTGGATGAGTACCGCCCGATTATTGGTTCGAAGCGTGATGTAATTTATTCCCTTGTTGATATTCTTAAGTATAGGAAATCTCCTCAGAGATCGATCAAGGATGCTTTGAAAGCGTTGTTCGGGATTGCGCTTCACCAATCGAATCGGTCTACTATGGTCGATCTCGGAGTGATTCCGCCGCTATTCTCGTTAGTTGTAGTCGGCGGTCACGCTGGGATTGTTGAGGATGCGTCGGCGGTGGTTGCGCAAGTGGCTGGATGTGAAGAGAGTGAATTGGCATTTCGTAGAGTTTCTGGATTGGGGGTTCTGGTGGATTTGTTGGATTCAGGGACTGGTTCGAGTCTGAGGACGAAGGAGAACGCCGTATCGGCGTTGTTGAATTTAGCAAAGCGGGGCGGAGATAGGGCAGCGGAGGATGTCAAGGATTTGGGATCTGGAATCTTAAGCGAAATTGCAGATATGGCCGTGAACGGCAGCGAGAAGGGGAAAACCAAGGCGGTGGAATTGTTGAAGATGATTTCAAGTGGCGGCATCGATGGTAATGTATTTGAGGATCTGAACTTGGATCGTTTGATAAATCCATGCTCTGAATAA

mRNA sequence

ATGGAGGTAAAGCGCCGTACAGCTTCCACTTTAGTCTCTCGCCTCAGCTCCGTCTCCGAGCAAATCCGGTGCGAATCCTTGTCGGAGCTCCGATTGATGACCAAGAACGACGCCGAAAGCCGATCACTTATCGTCGAAGCCGGTGCCATTCCTTACTTGGCTGAAACTCTGTATTCCTCTTCTCACGCCGCCCAAGAAGACGCCGCCGCTACGCTTCTCAACCTCTCAATCTCCTCCCGTGAAGCTTTAATGTCCACTCGCGGCCTCCTCGACGCGATTTCTCATGCACTTAGCCATCACAATTCCTCTTCCTCCGCCTCCGCCGTCCAATCCTGTGCTGCGACTCTCCATAGTCTTCTCGTTGTGGATGAGTACCGCCCGATTATTGGTTCGAAGCGTGATGTAATTTATTCCCTTGTTGATATTCTTAAGTATAGGAAATCTCCTCAGAGATCGATCAAGGATGCTTTGAAAGCGTTGTTCGGGATTGCGCTTCACCAATCGAATCGGTCTACTATGGTCGATCTCGGAGTGATTCCGCCGCTATTCTCGTTAGTTGTAGTCGGCGGTCACGCTGGGATTGTTGAGGATGCGTCGGCGGTGGTTGCGCAAGTGGCTGGATGTGAAGAGAGTGAATTGGCATTTCGTAGAGTTTCTGGATTGGGGGTTCTGGTGGATTTGTTGGATTCAGGGACTGGTTCGAGTCTGAGGACGAAGGAGAACGCCGTATCGGCGTTGTTGAATTTAGCAAAGCGGGGCGGAGATAGGGCAGCGGAGGATGTCAAGGATTTGGGATCTGGAATCTTAAGCGAAATTGCAGATATGGCCGTGAACGGCAGCGAGAAGGGGAAAACCAAGGCGGTGGAATTGTTGAAGATGATTTCAAGTGGCGGCATCGATGGTAATGTATTTGAGGATCTGAACTTGGATCGTTTGATAAATCCATGCTCTGAATAA

Coding sequence (CDS)

ATGGAGGTAAAGCGCCGTACAGCTTCCACTTTAGTCTCTCGCCTCAGCTCCGTCTCCGAGCAAATCCGGTGCGAATCCTTGTCGGAGCTCCGATTGATGACCAAGAACGACGCCGAAAGCCGATCACTTATCGTCGAAGCCGGTGCCATTCCTTACTTGGCTGAAACTCTGTATTCCTCTTCTCACGCCGCCCAAGAAGACGCCGCCGCTACGCTTCTCAACCTCTCAATCTCCTCCCGTGAAGCTTTAATGTCCACTCGCGGCCTCCTCGACGCGATTTCTCATGCACTTAGCCATCACAATTCCTCTTCCTCCGCCTCCGCCGTCCAATCCTGTGCTGCGACTCTCCATAGTCTTCTCGTTGTGGATGAGTACCGCCCGATTATTGGTTCGAAGCGTGATGTAATTTATTCCCTTGTTGATATTCTTAAGTATAGGAAATCTCCTCAGAGATCGATCAAGGATGCTTTGAAAGCGTTGTTCGGGATTGCGCTTCACCAATCGAATCGGTCTACTATGGTCGATCTCGGAGTGATTCCGCCGCTATTCTCGTTAGTTGTAGTCGGCGGTCACGCTGGGATTGTTGAGGATGCGTCGGCGGTGGTTGCGCAAGTGGCTGGATGTGAAGAGAGTGAATTGGCATTTCGTAGAGTTTCTGGATTGGGGGTTCTGGTGGATTTGTTGGATTCAGGGACTGGTTCGAGTCTGAGGACGAAGGAGAACGCCGTATCGGCGTTGTTGAATTTAGCAAAGCGGGGCGGAGATAGGGCAGCGGAGGATGTCAAGGATTTGGGATCTGGAATCTTAAGCGAAATTGCAGATATGGCCGTGAACGGCAGCGAGAAGGGGAAAACCAAGGCGGTGGAATTGTTGAAGATGATTTCAAGTGGCGGCATCGATGGTAATGTATTTGAGGATCTGAACTTGGATCGTTTGATAAATCCATGCTCTGAATAA

Protein sequence

MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGIDGNVFEDLNLDRLINPCSE
Homology
BLAST of Lsi08G009220 vs. ExPASy Swiss-Prot
Match: A2ZLU6 (Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 4.6e-28
Identity = 99/293 (33.79%), Postives = 160/293 (54.61%), Query Frame = 0

Query: 4   KRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHA 63
           +R     L+S+L S   + +  + +ELRL+ K +A +R  I EAGAIP L   L SS   
Sbjct: 365 ERANIDALLSKLCSPDTEEQRSAAAELRLLAKRNANNRICIAEAGAIPLLLSLLSSSDLR 424

Query: 64  AQEDAAATLLNLSI-SSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVV 123
            QE A   LLNLSI    +A + + G + +I H L     + S  A ++ AATL SL V+
Sbjct: 425 TQEHAVTALLNLSIHEDNKASIISSGAVPSIVHVL----KNGSMEARENAAATLFSLSVI 484

Query: 124 DEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPL 183
           DEY+  IG     I +LV +L   +  QR  KDA  ALF + ++Q N+   +  G++P +
Sbjct: 485 DEYKVTIGG-MGAIPALVVLL--GEGSQRGKKDAAAALFNLCIYQGNKGRAIRAGLVPLI 544

Query: 184 FSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENA 243
             L V      ++++A A+++ ++   E + A      + VLV+++ SGT    R +ENA
Sbjct: 545 MGL-VTNPTGALMDEAMAILSILSSHPEGKAAIGAAEPVPVLVEMIGSGTP---RNRENA 604

Query: 244 VSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMIS 296
            + +L+L   G        +    GI+  + ++A+NG+++GK KAV+LL+ +S
Sbjct: 605 AAVMLHLCS-GEHHLVHLARAQECGIMVPLRELALNGTDRGKRKAVQLLERMS 645

BLAST of Lsi08G009220 vs. ExPASy Swiss-Prot
Match: Q0IMG9 (E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SPL11 PE=1 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 4.6e-28
Identity = 99/293 (33.79%), Postives = 160/293 (54.61%), Query Frame = 0

Query: 4   KRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHA 63
           +R     L+S+L S   + +  + +ELRL+ K +A +R  I EAGAIP L   L SS   
Sbjct: 365 ERANIDALLSKLCSPDTEEQRSAAAELRLLAKRNANNRICIAEAGAIPLLLSLLSSSDLR 424

Query: 64  AQEDAAATLLNLSI-SSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVV 123
            QE A   LLNLSI    +A + + G + +I H L     + S  A ++ AATL SL V+
Sbjct: 425 TQEHAVTALLNLSIHEDNKASIISSGAVPSIVHVL----KNGSMEARENAAATLFSLSVI 484

Query: 124 DEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPL 183
           DEY+  IG     I +LV +L   +  QR  KDA  ALF + ++Q N+   +  G++P +
Sbjct: 485 DEYKVTIGG-MGAIPALVVLL--GEGSQRGKKDAAAALFNLCIYQGNKGRAIRAGLVPLI 544

Query: 184 FSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENA 243
             L V      ++++A A+++ ++   E + A      + VLV+++ SGT    R +ENA
Sbjct: 545 MGL-VTNPTGALMDEAMAILSILSSHPEGKAAIGAAEPVPVLVEMIGSGTP---RNRENA 604

Query: 244 VSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMIS 296
            + +L+L   G        +    GI+  + ++A+NG+++GK KAV+LL+ +S
Sbjct: 605 AAVMLHLCS-GEHHLVHLARAQECGIMVPLRELALNGTDRGKRKAVQLLERMS 645

BLAST of Lsi08G009220 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 118.6 bits (296), Expect = 1.3e-25
Identity = 94/286 (32.87%), Postives = 157/286 (54.90%), Query Frame = 0

Query: 11  LVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDAAA 70
           LV RLSS S + R  ++SE+R ++K   ++R LI EAGAIP L   L S   A QE+A  
Sbjct: 336 LVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENAIT 395

Query: 71  TLLNLSI-SSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRPII 130
            +LNLSI  + + L+   G + +I   L     + +  A ++ AATL SL + DE + II
Sbjct: 396 CVLNLSIYENNKELIMFAGAVTSIVQVL----RAGTMEARENAAATLFSLSLADENKIII 455

Query: 131 GSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVVVG 190
           G     I +LVD+L+      R  KDA  ALF + ++  N+   V  G++  L  ++   
Sbjct: 456 GGS-GAIPALVDLLE--NGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDS 515

Query: 191 GHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALLNL 250
               +V++A  +++ +A  ++++ A  + + L  L+ +L +      R +ENA + LL+L
Sbjct: 516 TRHRMVDEALTILSVLANNQDAKSAIVKANTLPALIGILQT---DQTRNRENAAAILLSL 575

Query: 251 AKRGGDRAAEDVKDLGS-GILSEIADMAVNGSEKGKTKAVELLKMI 295
            KR      E +  +G  G +  + D++ NG+E+GK KA+ LL+++
Sbjct: 576 CKRD----TEKLITIGRLGAVVPLMDLSKNGTERGKRKAISLLELL 607

BLAST of Lsi08G009220 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 115.2 bits (287), Expect = 1.4e-24
Identity = 98/282 (34.75%), Postives = 157/282 (55.67%), Query Frame = 0

Query: 11  LVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDAAA 70
           LV  L S S   + ++ +ELRL+ K++ ++R +I  +GAI  L E LYS+  A QE+A  
Sbjct: 546 LVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQENAVT 605

Query: 71  TLLNLSIS-SREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRPII 130
            LLNLSI+ + +  ++  G ++ + H L     + S+ A ++ AATL SL V++E +  I
Sbjct: 606 ALLNLSINDNNKKAIADAGAIEPLIHVL----ENGSSEAKENSAATLFSLSVIEENKIKI 665

Query: 131 GSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVVVG 190
           G +   I  LVD+L       R  KDA  ALF +++HQ N++ +V  G +  L  L  + 
Sbjct: 666 G-QSGAIGPLVDLL--GNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDL--MD 725

Query: 191 GHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALLNL 250
             AG+V+ A AV+A +A   E   A  +  G+ +LV++++ G   S R KENA +ALL L
Sbjct: 726 PAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELG---SARGKENAAAALLQL 785

Query: 251 AKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELL 292
           +   G R    V  L  G +  +  ++ +G+ + + KA  LL
Sbjct: 786 STNSG-RFCNMV--LQEGAVPPLVALSQSGTPRAREKAQALL 812

BLAST of Lsi08G009220 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.6e-23
Identity = 94/286 (32.87%), Postives = 151/286 (52.80%), Query Frame = 0

Query: 11  LVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDAAA 70
           L+ RL+  + + +  +  E+RL+ K +A++R  I EAGAIP L   L +     QE +  
Sbjct: 357 LMWRLAYGNPEDQRSAAGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVT 416

Query: 71  TLLNLSI-SSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRPII 130
            LLNLSI  + +  + + G +  I   L       S  A ++ AATL SL V+DE +  I
Sbjct: 417 ALLNLSICENNKGAIVSAGAIPGIVQVL----KKGSMEARENAAATLFSLSVIDENKVTI 476

Query: 131 GSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVVVG 190
           G+    I  LV +L   +  QR  KDA  ALF + ++Q N+   +  GVIP L  L+   
Sbjct: 477 GA-LGAIPPLVVLL--NEGTQRGKKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEP 536

Query: 191 GHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALLNL 250
           G +G+V++A A++A ++   E +        +  LV+ + +G   S R +ENA + L++L
Sbjct: 537 G-SGMVDEALAILAILSSHPEGKAIIGSSDAVPSLVEFIRTG---SPRNRENAAAVLVHL 596

Query: 251 AKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMIS 296
                    E  K    G++  + D+A NG+++GK KA +LL+ IS
Sbjct: 597 CSGDPQHLVEAQK---LGLMGPLIDLAGNGTDRGKRKAAQLLERIS 628

BLAST of Lsi08G009220 vs. ExPASy TrEMBL
Match: A0A5A7VB10 (Protein spotted leaf 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00950 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.7e-155
Identity = 301/318 (94.65%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVKRRT STLVSRLSSVSEQ RCESLSELRLMTKND ESRSLIVEAGA+PYL+ETLYSS
Sbjct: 1   MEVKRRTVSTLVSRLSSVSEQTRCESLSELRLMTKNDVESRSLIVEAGALPYLSETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SH AQEDAAATLLNLSISSR+ALMST GLLDAISH LSHHNSSSSASAVQSC ATLHSLL
Sbjct: 61  SHVAQEDAAATLLNLSISSRQALMSTHGLLDAISHVLSHHNSSSSASAVQSCGATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE
Sbjct: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLAK GGDRAAEDVKDLGSGILSEIAD+AVNGSEKGKTKAVELLKM++SGGID
Sbjct: 241 NAVSALLNLAKWGGDRAAEDVKDLGSGILSEIADVAVNGSEKGKTKAVELLKMVASGGID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDL L+RLINPCSE
Sbjct: 301 GNVFEDLQLNRLINPCSE 318

BLAST of Lsi08G009220 vs. ExPASy TrEMBL
Match: A0A1S3BCM9 (protein spotted leaf 11 OS=Cucumis melo OX=3656 GN=LOC103488443 PE=4 SV=1)

HSP 1 Score: 556.6 bits (1433), Expect = 6.7e-155
Identity = 301/318 (94.65%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVKRRT STLVSRLSSVSEQ RCESLSELRLMTKND ESRSLIVEAGA+PYL+ETLYSS
Sbjct: 1   MEVKRRTVSTLVSRLSSVSEQTRCESLSELRLMTKNDVESRSLIVEAGALPYLSETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SH AQEDAAATLLNLSISSR+ALMST GLLDAISH LSHHNSSSSASAVQSC ATLHSLL
Sbjct: 61  SHVAQEDAAATLLNLSISSRQALMSTHGLLDAISHVLSHHNSSSSASAVQSCGATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE
Sbjct: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLAK GGDRAAEDVKDLGSGILSEIAD+AVNGSEKGKTKAVELLKM++SGGID
Sbjct: 241 NAVSALLNLAKWGGDRAAEDVKDLGSGILSEIADVAVNGSEKGKTKAVELLKMVASGGID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDL L+RLINPCSE
Sbjct: 301 GNVFEDLQLNRLINPCSE 318

BLAST of Lsi08G009220 vs. ExPASy TrEMBL
Match: A0A0A0KDY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G401390 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.4e-152
Identity = 297/318 (93.40%), Postives = 307/318 (96.54%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVK R  STLVSRLSSVSEQIRCESLSELRLMTKNDA+SRSLIV AGA+PYL+ETLYSS
Sbjct: 1   MEVKGRAVSTLVSRLSSVSEQIRCESLSELRLMTKNDAQSRSLIVHAGALPYLSETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SH  QEDAAATLLNLSISSREALMST GLLDAISH LSHHNSSSS+SAVQSCAATLHSLL
Sbjct: 61  SHLPQEDAAATLLNLSISSREALMSTHGLLDAISHVLSHHNSSSSSSAVQSCAATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE
Sbjct: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLAK GGDRAAEDVKDLGSGILSEIAD+AVNGSEKGKTKAVELLKM++SGGID
Sbjct: 241 NAVSALLNLAKWGGDRAAEDVKDLGSGILSEIADVAVNGSEKGKTKAVELLKMVASGGID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           G VF+DL L+RLINPCSE
Sbjct: 301 GKVFDDLQLNRLINPCSE 318

BLAST of Lsi08G009220 vs. ExPASy TrEMBL
Match: A0A6J1GIK8 (protein spotted leaf 11 OS=Cucurbita moschata OX=3662 GN=LOC111454225 PE=4 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 1.8e-147
Identity = 289/318 (90.88%), Postives = 302/318 (94.97%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVKRRTASTLVSRLSSVSEQIRC+SLSELRLMTKNDAESRSLI E GAIPYLAETLYSS
Sbjct: 1   MEVKRRTASTLVSRLSSVSEQIRCDSLSELRLMTKNDAESRSLIAETGAIPYLAETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SHAAQEDAAATLLNLSISSREALMST GLLDAISH L HHNSSSSA+AVQSCAATLHSLL
Sbjct: 61  SHAAQEDAAATLLNLSISSREALMSTHGLLDAISHVLGHHNSSSSAAAVQSCAATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILK+RKSPQRSIKDALKALFGIAL Q NRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKFRKSPQRSIKDALKALFGIALCQPNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVG HAGIVEDASAVVAQVAGCE+SELAFR+VSGLGVLVDLLDSGTGSS RTKE
Sbjct: 181 PLFSLVVVGSHAGIVEDASAVVAQVAGCEDSELAFRKVSGLGVLVDLLDSGTGSSQRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLA+ GG+RA ED+KDLGSGILSEI D+A+NGSEKGK KAVELLK+I+SG ID
Sbjct: 241 NAVSALLNLARWGGERAEEDIKDLGSGILSEIGDVAMNGSEKGKAKAVELLKLIASGDID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDL LDRLIN CSE
Sbjct: 301 GNVFEDLQLDRLINSCSE 318

BLAST of Lsi08G009220 vs. ExPASy TrEMBL
Match: A0A6J1KPD1 (protein spotted leaf 11 OS=Cucurbita maxima OX=3661 GN=LOC111496043 PE=4 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 3.0e-147
Identity = 288/318 (90.57%), Postives = 302/318 (94.97%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVK RTASTLVSRLSSVSEQIRC+SLSELRLMTKNDAESRSLI E GAIPYLAETLYSS
Sbjct: 78  MEVKLRTASTLVSRLSSVSEQIRCDSLSELRLMTKNDAESRSLIAETGAIPYLAETLYSS 137

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SHAAQEDAAATLLNLSISSREALMST GLLDAISH L HHNSSSSA+AVQSCAATLHSLL
Sbjct: 138 SHAAQEDAAATLLNLSISSREALMSTHGLLDAISHVLGHHNSSSSAAAVQSCAATLHSLL 197

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVD+YRPIIGSKRD+IYSLVDILK+RKSPQRSIKDALKALFGIAL+Q NRSTMVDLGVIP
Sbjct: 198 VVDDYRPIIGSKRDIIYSLVDILKFRKSPQRSIKDALKALFGIALYQPNRSTMVDLGVIP 257

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVG HAGIVEDASAVVAQVAGCE+SELAFRRVSGLGVLVDLLDSGTGSS RTKE
Sbjct: 258 PLFSLVVVGSHAGIVEDASAVVAQVAGCEDSELAFRRVSGLGVLVDLLDSGTGSSQRTKE 317

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLA+ GG+RA ED+KDLGSGILSEI D+A+NGSEKGK KAVELLK+I+SG ID
Sbjct: 318 NAVSALLNLARWGGERAEEDIKDLGSGILSEIGDVAMNGSEKGKAKAVELLKLIASGDID 377

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDL LDRLIN CSE
Sbjct: 378 GNVFEDLQLDRLINSCSE 395

BLAST of Lsi08G009220 vs. NCBI nr
Match: XP_038884040.1 (protein spotted leaf 11 [Benincasa hispida])

HSP 1 Score: 568.2 bits (1463), Expect = 4.6e-158
Identity = 310/318 (97.48%), Postives = 314/318 (98.74%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS
Sbjct: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHAL HHNSSSSASAVQSCAATLHSLL
Sbjct: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALGHHNSSSSASAVQSCAATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSS RTKE
Sbjct: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSQRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLAK GGDRAAEDVKDLGSGILSEIA++AVNGSEKGK KAVELLK+ISSGGID
Sbjct: 241 NAVSALLNLAKWGGDRAAEDVKDLGSGILSEIAEVAVNGSEKGKNKAVELLKIISSGGID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDLNLDRLINPCSE
Sbjct: 301 GNVFEDLNLDRLINPCSE 318

BLAST of Lsi08G009220 vs. NCBI nr
Match: XP_008445408.1 (PREDICTED: protein spotted leaf 11 [Cucumis melo] >KAA0064788.1 protein spotted leaf 11 [Cucumis melo var. makuwa])

HSP 1 Score: 556.6 bits (1433), Expect = 1.4e-154
Identity = 301/318 (94.65%), Postives = 309/318 (97.17%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVKRRT STLVSRLSSVSEQ RCESLSELRLMTKND ESRSLIVEAGA+PYL+ETLYSS
Sbjct: 1   MEVKRRTVSTLVSRLSSVSEQTRCESLSELRLMTKNDVESRSLIVEAGALPYLSETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SH AQEDAAATLLNLSISSR+ALMST GLLDAISH LSHHNSSSSASAVQSC ATLHSLL
Sbjct: 61  SHVAQEDAAATLLNLSISSRQALMSTHGLLDAISHVLSHHNSSSSASAVQSCGATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE
Sbjct: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLAK GGDRAAEDVKDLGSGILSEIAD+AVNGSEKGKTKAVELLKM++SGGID
Sbjct: 241 NAVSALLNLAKWGGDRAAEDVKDLGSGILSEIADVAVNGSEKGKTKAVELLKMVASGGID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDL L+RLINPCSE
Sbjct: 301 GNVFEDLQLNRLINPCSE 318

BLAST of Lsi08G009220 vs. NCBI nr
Match: XP_004146768.1 (protein spotted leaf 11 [Cucumis sativus] >KGN47768.1 hypothetical protein Csa_004083 [Cucumis sativus])

HSP 1 Score: 548.1 bits (1411), Expect = 4.9e-152
Identity = 297/318 (93.40%), Postives = 307/318 (96.54%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVK R  STLVSRLSSVSEQIRCESLSELRLMTKNDA+SRSLIV AGA+PYL+ETLYSS
Sbjct: 1   MEVKGRAVSTLVSRLSSVSEQIRCESLSELRLMTKNDAQSRSLIVHAGALPYLSETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SH  QEDAAATLLNLSISSREALMST GLLDAISH LSHHNSSSS+SAVQSCAATLHSLL
Sbjct: 61  SHLPQEDAAATLLNLSISSREALMSTHGLLDAISHVLSHHNSSSSSSAVQSCAATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE
Sbjct: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLAK GGDRAAEDVKDLGSGILSEIAD+AVNGSEKGKTKAVELLKM++SGGID
Sbjct: 241 NAVSALLNLAKWGGDRAAEDVKDLGSGILSEIADVAVNGSEKGKTKAVELLKMVASGGID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           G VF+DL L+RLINPCSE
Sbjct: 301 GKVFDDLQLNRLINPCSE 318

BLAST of Lsi08G009220 vs. NCBI nr
Match: KAG7020188.1 (U-box domain-containing protein 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 533.1 bits (1372), Expect = 1.6e-147
Identity = 290/318 (91.19%), Postives = 302/318 (94.97%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLI E GAIPYLAETLYSS
Sbjct: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIAETGAIPYLAETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SHAAQEDAAATLLNLSISSREALMST GLLDAISH L HHNSSSSA+AVQSCAATLHSLL
Sbjct: 61  SHAAQEDAAATLLNLSISSREALMSTHGLLDAISHVLGHHNSSSSAAAVQSCAATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRPIIGSKRD+IYSLVDILK+RKSPQRSIKDALKALFGIAL Q NRSTMVDLGVIP
Sbjct: 121 VVDEYRPIIGSKRDIIYSLVDILKFRKSPQRSIKDALKALFGIALCQPNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVG HAGIVEDASAVVAQVAGCE+SELAFR+VSGLGVLVDLLDSGTGSS RTKE
Sbjct: 181 PLFSLVVVGSHAGIVEDASAVVAQVAGCEDSELAFRKVSGLGVLVDLLDSGTGSSQRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLA+ GG+RA ED+KDLGSGILSEI D+A+NGSEKGK KAVELLK+I+SG ID
Sbjct: 241 NAVSALLNLARWGGERAEEDIKDLGSGILSEIGDVAMNGSEKGKAKAVELLKLIASGDID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDL LDRLIN CSE
Sbjct: 301 GNVFEDLQLDRLINSCSE 318

BLAST of Lsi08G009220 vs. NCBI nr
Match: XP_023538067.1 (protein spotted leaf 11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532.7 bits (1371), Expect = 2.1e-147
Identity = 289/318 (90.88%), Postives = 302/318 (94.97%), Query Frame = 0

Query: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSS 60
           MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLI E GAIPYLAETLYSS
Sbjct: 1   MEVKRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIAETGAIPYLAETLYSS 60

Query: 61  SHAAQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLL 120
           SHAAQEDAAATLLNLSISSREALMST GLLDAISH L HHNSSSSA+AVQSCAATLHSLL
Sbjct: 61  SHAAQEDAAATLLNLSISSREALMSTHGLLDAISHVLGHHNSSSSAAAVQSCAATLHSLL 120

Query: 121 VVDEYRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIP 180
           VVDEYRP+IGSKRD+IYSLVDILK+RKSPQRSIKDALKALFGIAL Q NRSTMVDLGVIP
Sbjct: 121 VVDEYRPVIGSKRDIIYSLVDILKFRKSPQRSIKDALKALFGIALCQPNRSTMVDLGVIP 180

Query: 181 PLFSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKE 240
           PLFSLVVVG HAGIVEDASAVVAQVAGCE+SELAFR+VSGLGVLVDLLDSGTGSS RTKE
Sbjct: 181 PLFSLVVVGSHAGIVEDASAVVAQVAGCEDSELAFRKVSGLGVLVDLLDSGTGSSQRTKE 240

Query: 241 NAVSALLNLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSGGID 300
           NAVSALLNLA+ GG+RA ED+KDLGSGILSEI D+A+NGSEKGK KAVELLK+I+SG ID
Sbjct: 241 NAVSALLNLARWGGERAEEDIKDLGSGILSEIGDVAMNGSEKGKAKAVELLKLIASGDID 300

Query: 301 GNVFEDLNLDRLINPCSE 319
           GNVFEDL LDRLIN CSE
Sbjct: 301 GNVFEDLQLDRLINSCSE 318

BLAST of Lsi08G009220 vs. TAIR 10
Match: AT1G08315.1 (ARM repeat superfamily protein )

HSP 1 Score: 339.0 bits (868), Expect = 4.2e-93
Identity = 197/317 (62.15%), Postives = 241/317 (76.03%), Query Frame = 0

Query: 4   KRRTASTLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHA 63
           KRRT ST+VSRLSSVSEQ R  +L+ELRL++K D +SR +I +AGAIPYLAETLYSSSH+
Sbjct: 5   KRRTMSTIVSRLSSVSEQTRAAALAELRLISKQDPDSRLIIADAGAIPYLAETLYSSSHS 64

Query: 64  AQEDAAATLLNLSISSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVD 123
           +QE+AAATLLNLSI+SRE LMS+RGLLDA+SHAL HH++++S +AVQS AAT++SLL+ +
Sbjct: 65  SQENAAATLLNLSITSREPLMSSRGLLDALSHALRHHDTTTSPAAVQSSAATIYSLLIAE 124

Query: 124 E-YRPIIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPL 183
           E YRPIIGSKRD+I+SL+ I++Y  S  RSIKD+LKALF IAL+  NRSTM+ LG IP L
Sbjct: 125 ESYRPIIGSKRDIIFSLIHIIRYPDSHPRSIKDSLKALFAIALYPMNRSTMISLGAIPAL 184

Query: 184 FSLVVVGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENA 243
           FSL+V     GIVEDA+AV+AQVAGCE+SE   RRVSG  VL DLLD  TGSSLR KEN+
Sbjct: 185 FSLIVKDSRCGIVEDATAVMAQVAGCEDSEDGMRRVSGANVLADLLDPCTGSSLRIKENS 244

Query: 244 VSALLNLAKRGGDRAAEDV-----KDLGSGILSEIADMAVNGSEKGKTKAVELLKMISSG 303
           V ALLNLA+ GG  A  +V          G +  I  +A NGS KG+ KAV+LLK++ SG
Sbjct: 245 VGALLNLARCGGAAARSEVAAAVASGADEGAMEGIVYVAENGSLKGRKKAVDLLKLVVSG 304

Query: 304 GIDGNVFEDLNLDRLIN 315
              G    D   D L N
Sbjct: 305 NGGG----DSRFDYLFN 317

BLAST of Lsi08G009220 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 9.0e-27
Identity = 94/286 (32.87%), Postives = 157/286 (54.90%), Query Frame = 0

Query: 11  LVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDAAA 70
           LV RLSS S + R  ++SE+R ++K   ++R LI EAGAIP L   L S   A QE+A  
Sbjct: 336 LVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENAIT 395

Query: 71  TLLNLSI-SSREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRPII 130
            +LNLSI  + + L+   G + +I   L     + +  A ++ AATL SL + DE + II
Sbjct: 396 CVLNLSIYENNKELIMFAGAVTSIVQVL----RAGTMEARENAAATLFSLSLADENKIII 455

Query: 131 GSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVVVG 190
           G     I +LVD+L+      R  KDA  ALF + ++  N+   V  G++  L  ++   
Sbjct: 456 GGS-GAIPALVDLLE--NGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKMLSDS 515

Query: 191 GHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALLNL 250
               +V++A  +++ +A  ++++ A  + + L  L+ +L +      R +ENA + LL+L
Sbjct: 516 TRHRMVDEALTILSVLANNQDAKSAIVKANTLPALIGILQT---DQTRNRENAAAILLSL 575

Query: 251 AKRGGDRAAEDVKDLGS-GILSEIADMAVNGSEKGKTKAVELLKMI 295
            KR      E +  +G  G +  + D++ NG+E+GK KA+ LL+++
Sbjct: 576 CKRD----TEKLITIGRLGAVVPLMDLSKNGTERGKRKAISLLELL 607

BLAST of Lsi08G009220 vs. TAIR 10
Match: AT2G23140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 115.2 bits (287), Expect = 9.9e-26
Identity = 98/282 (34.75%), Postives = 157/282 (55.67%), Query Frame = 0

Query: 11  LVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDAAA 70
           LV  L S S   + ++ +ELRL+ K++ ++R +I  +GAI  L E LYS+  A QE+A  
Sbjct: 549 LVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQENAVT 608

Query: 71  TLLNLSIS-SREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRPII 130
            LLNLSI+ + +  ++  G ++ + H L     + S+ A ++ AATL SL V++E +  I
Sbjct: 609 ALLNLSINDNNKKAIADAGAIEPLIHVL----ENGSSEAKENSAATLFSLSVIEENKIKI 668

Query: 131 GSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVVVG 190
           G +   I  LVD+L       R  KDA  ALF +++HQ N++ +V  G +  L  L  + 
Sbjct: 669 G-QSGAIGPLVDLL--GNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDL--MD 728

Query: 191 GHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALLNL 250
             AG+V+ A AV+A +A   E   A  +  G+ +LV++++ G   S R KENA +ALL L
Sbjct: 729 PAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELG---SARGKENAAAALLQL 788

Query: 251 AKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELL 292
           +   G R    V  L  G +  +  ++ +G+ + + KA  LL
Sbjct: 789 STNSG-RFCNMV--LQEGAVPPLVALSQSGTPRAREKAQALL 815

BLAST of Lsi08G009220 vs. TAIR 10
Match: AT2G23140.2 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 115.2 bits (287), Expect = 9.9e-26
Identity = 98/282 (34.75%), Postives = 157/282 (55.67%), Query Frame = 0

Query: 11  LVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDAAA 70
           LV  L S S   + ++ +ELRL+ K++ ++R +I  +GAI  L E LYS+  A QE+A  
Sbjct: 546 LVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLYSTDSATQENAVT 605

Query: 71  TLLNLSIS-SREALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRPII 130
            LLNLSI+ + +  ++  G ++ + H L     + S+ A ++ AATL SL V++E +  I
Sbjct: 606 ALLNLSINDNNKKAIADAGAIEPLIHVL----ENGSSEAKENSAATLFSLSVIEENKIKI 665

Query: 131 GSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVVVG 190
           G +   I  LVD+L       R  KDA  ALF +++HQ N++ +V  G +  L  L  + 
Sbjct: 666 G-QSGAIGPLVDLL--GNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLIDL--MD 725

Query: 191 GHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALLNL 250
             AG+V+ A AV+A +A   E   A  +  G+ +LV++++ G   S R KENA +ALL L
Sbjct: 726 PAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELG---SARGKENAAAALLQL 785

Query: 251 AKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELL 292
           +   G R    V  L  G +  +  ++ +G+ + + KA  LL
Sbjct: 786 STNSG-RFCNMV--LQEGAVPPLVALSQSGTPRAREKAQALL 812

BLAST of Lsi08G009220 vs. TAIR 10
Match: AT5G42340.1 (Plant U-Box 15 )

HSP 1 Score: 111.7 bits (278), Expect = 1.1e-24
Identity = 92/288 (31.94%), Postives = 155/288 (53.82%), Query Frame = 0

Query: 9   STLVSRLSSVSEQIRCESLSELRLMTKNDAESRSLIVEAGAIPYLAETLYSSSHAAQEDA 68
           S LV  LSS   + +  S+ ++RL+ + + E+R LI  AGAIP L + L       QE+A
Sbjct: 382 SLLVEALSSSQLEEQRRSVKQMRLLARENPENRVLIANAGAIPLLVQLLSYPDSGIQENA 441

Query: 69  AATLLNLSISS-REALMSTRGLLDAISHALSHHNSSSSASAVQSCAATLHSLLVVDEYRP 128
             TLLNLSI    + L+S  G +  I   L + N      A ++ AA L SL ++DE + 
Sbjct: 442 VTTLLNLSIDEVNKKLISNEGAIPNIIEILENGN----REARENSAAALFSLSMLDENKV 501

Query: 129 IIGSKRDVIYSLVDILKYRKSPQRSIKDALKALFGIALHQSNRSTMVDLGVIPPLFSLVV 188
            IG     I  LVD+L++     R  KDAL ALF ++L+ +N+   +D G++ PL +L +
Sbjct: 502 TIGLSNG-IPPLVDLLQH--GTLRGKKDALTALFNLSLNSANKGRAIDAGIVQPLLNL-L 561

Query: 189 VGGHAGIVEDASAVVAQVAGCEESELAFRRVSGLGVLVDLLDSGTGSSLRTKENAVSALL 248
              + G++++A +++  +A   E   A  ++S +  LV+ +  GT    + KE A S LL
Sbjct: 562 KDKNLGMIDEALSILLLLASHPEGRQAIGQLSFIETLVEFIRQGTP---KNKECATSVLL 621

Query: 249 NLAKRGGDRAAEDVKDLGSGILSEIADMAVNGSEKGKTKAVELLKMIS 296
            L   G + ++  +  L  G+   + ++  +G+ + + KA  L+++IS
Sbjct: 622 EL---GSNNSSFILAALQFGVYEYLVEITTSGTNRAQRKANALIQLIS 655

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2ZLU64.6e-2833.79Protein spotted leaf 11 OS=Oryza sativa subsp. indica OX=39946 GN=SPL11 PE=1 SV=... [more]
Q0IMG94.6e-2833.79E3 ubiquitin-protein ligase SPL11 OS=Oryza sativa subsp. japonica OX=39947 GN=SP... [more]
Q8GUG91.3e-2532.87U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... [more]
O221931.4e-2434.75U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 S... [more]
Q9SNC62.6e-2332.87U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5A7VB106.7e-15594.65Protein spotted leaf 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3BCM96.7e-15594.65protein spotted leaf 11 OS=Cucumis melo OX=3656 GN=LOC103488443 PE=4 SV=1[more]
A0A0A0KDY42.4e-15293.40Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G401390 PE=4 SV=1[more]
A0A6J1GIK81.8e-14790.88protein spotted leaf 11 OS=Cucurbita moschata OX=3662 GN=LOC111454225 PE=4 SV=1[more]
A0A6J1KPD13.0e-14790.57protein spotted leaf 11 OS=Cucurbita maxima OX=3661 GN=LOC111496043 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038884040.14.6e-15897.48protein spotted leaf 11 [Benincasa hispida][more]
XP_008445408.11.4e-15494.65PREDICTED: protein spotted leaf 11 [Cucumis melo] >KAA0064788.1 protein spotted ... [more]
XP_004146768.14.9e-15293.40protein spotted leaf 11 [Cucumis sativus] >KGN47768.1 hypothetical protein Csa_0... [more]
KAG7020188.11.6e-14791.19U-box domain-containing protein 11, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_023538067.12.1e-14790.88protein spotted leaf 11 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G08315.14.2e-9362.15ARM repeat superfamily protein [more]
AT1G23030.19.0e-2732.87ARM repeat superfamily protein [more]
AT2G23140.19.9e-2634.75RING/U-box superfamily protein with ARM repeat domain [more]
AT2G23140.29.9e-2634.75RING/U-box superfamily protein with ARM repeat domain [more]
AT5G42340.11.1e-2431.94Plant U-Box 15 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 166..207
e-value: 1.2
score: 18.2
coord: 208..251
e-value: 0.06
score: 22.5
coord: 37..77
e-value: 0.028
score: 23.6
IPR000225ArmadilloPFAMPF00514Armcoord: 39..76
e-value: 2.3E-5
score: 24.2
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 3..310
e-value: 3.3E-32
score: 113.6
NoneNo IPR availablePANTHERPTHR23315:SF238ARM REPEAT SUPERFAMILY PROTEINcoord: 2..301
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 2..301
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 8..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G009220.1Lsi08G009220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding