Lsi08G008400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G008400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRWP-RK domain-containing protein
Locationchr08: 16862633 .. 16864649 (+)
RNA-Seq ExpressionLsi08G008400
SyntenyLsi08G008400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAACTGGAAATTCATGGTAGGCTGGGGATGATTTGCCATGCAATATTGGAGCATAAACCGATTGTTAATGTGGATAATATAAGTCCTCAGTATCAAATGTTCGAGTAAGAGATGAAATTTAAAAGTCGTTTTTTTTTTTTTTTGGTTTTAAATTGAATAAATGATGATATAATAAATTAATTAAATGGTATTGAAATTTATAATTAGTTTTTGCGAGAAAAGCTGTGAGGAGATAAAGCAATTTCTACTACAGTATTGTCTGAAGCAGATATTGGAAGATTACAATATGATTCCAGATCCAATGTCCAATTTCTACGATGCACTTTGTGTTGGAATTGATTGGTTTGAAAATCTTAATACCGATGGCTTCTTTCAACTCTCTCCCGACAATTCTGGTTAGTATTTTTTTTTTCCATTTTTAAATCTATTTATTTTAATTTAATTACAACTTTCAATTTCATTCATCACGTATATATATATTTAAGGAAATTTTCACAATAAAAATGTTACTATGTATAGAAAATATACTGATAGACATTGATACACTTCTATCTGTATATATATCAGTCATAGATTTCTATCATTTCTATTAGTGATAGAACCTGATAGACTTTTATTAGTGTCTATCACAACTATCTAAAAATTTTGCTATTTTGTGTAAATAGTTTTCCTTATTTTTCTATTTTTAAAAATACTCCAATATTTAAATATTTCAATGGTTATACTCAAACCCTCAAATTATGGAATGCTTATTTAACCAAACAATTAATTTAATTTGTGTAAAGAATTTGGGTCTGAATGTTATAATTTTATTGTTTGAGGGTTTGATTGGAATGACTAAAGGGTGTATACAGGTTGGGTTTGAGAGATCTTTTTGGACCAATATGAAAATTTGAGTTGGTTACCTAAAAGACCCGAACATGGTCTCTCTAACCCAACCCTTACAATTCAGTGAGTTGAGTTGTCGAGTTATTGTCTTTTCTTATTAATTTAAAATATTTAATTTTATTTACAATACATAACTAATGAGTAAAATCTTATAAATGTTAAATGTTAAATATCAAATATCGATAATATCTATTATAAATTTTAAAGAAAGATAAACATAGCAACAATCCTAGATGCTAAAAATTAAAACATAATATATATATAATTTGGGTTGGTTGGGTCAACCTGAAGAAAAAAATAATAATAAAAATCCGAGACTCGGCCAAACCAAGAAGAAAAAATTCAAACCAACTCAACCTTAGAACAAAACCAACCCAACCAGTTTAGATTGGATAATTCGAATTGTTCGGATTGTCGAGTCATTTGAATATAGTAGAATTAACCATTGATTTAACAAAAATATATCAATGGCTATGGTATACTATATTCTGCTGAATTCTGCGTCAAACCTCGGAGTTATTGCAGAAGATGATGACATGGAACAACCCATTCCAGATTTCCAAAATGAGCCACCGGAGCAACCGGCCGAGCAGCCCCCAAAACGACCCTCCCTAGCTGCACAGGTTCCTTTTTATAAATTCTCTCAATTTAAAATATGTTTTTATATTTTTGATTTTGTAGTCTCTAAAACTGTATAATACGTTGCTTTAAAACACCTTTTATAATTGAATTTTTCAGCGACAGAGAACGGGAAGGATGACAGTGAACGACGTGTGGGAATATCTCCATCTTCCAATATCAGAAGCTTCGAAGAAACTGAATGTGTGCAATACAGTGTTGAAGAAAATATGCCGCCGGAGCGGCCTCAGCCGATGGCCTTACAGAAAGGTATTTTATTCACTAATAATAATTAGTTATAAATATTATTAATATATTTTGTTAATTTGTAATTAATTAATAATTAATCTCACTTTTGGGCTGCGTAGATACGGAGTTATGAGAGACGAATAGCGGCGCTGAGAGCTACAATGGATTCAAGCTATGGAGATACCAGAATCCGGGCTGAAGCCGAGATTGAAAGGGTTCAAAAAGAACTTGCTGATTTTTGTGCTCGAATCAGAATTTGA

mRNA sequence

GAAAACTGGAAATTCATGGTAGGCTGGGGATGATTTGCCATGCAATATTGGAGCATAAACCGATTGTTAATGTGGATAATATAAGTCCTCAGTATCAAATGTTCGATTTTTGCGAGAAAAGCTGTGAGGAGATAAAGCAATTTCTACTACAGTATTGTCTGAAGCAGATATTGGAAGATTACAATATGATTCCAGATCCAATGTCCAATTTCTACGATGCACTTTGTGTTGGAATTGATTGGTTTGAAAATCTTAATACCGATGGCTTCTTTCAACTCTCTCCCGACAATTCTGAAGATGATGACATGGAACAACCCATTCCAGATTTCCAAAATGAGCCACCGGAGCAACCGGCCGAGCAGCCCCCAAAACGACCCTCCCTAGCTGCACAGCGACAGAGAACGGGAAGGATGACAGTGAACGACGTGTGGGAATATCTCCATCTTCCAATATCAGAAGCTTCGAAGAAACTGAATGTGTGCAATACAGTGTTGAAGAAAATATGCCGCCGGAGCGGCCTCAGCCGATGGCCTTACAGAAAGATACGGAGTTATGAGAGACGAATAGCGGCGCTGAGAGCTACAATGGATTCAAGCTATGGAGATACCAGAATCCGGGCTGAAGCCGAGATTGAAAGGGTTCAAAAAGAACTTGCTGATTTTTGTGCTCGAATCAGAATTTGA

Coding sequence (CDS)

ATGATTTGCCATGCAATATTGGAGCATAAACCGATTGTTAATGTGGATAATATAAGTCCTCAGTATCAAATGTTCGATTTTTGCGAGAAAAGCTGTGAGGAGATAAAGCAATTTCTACTACAGTATTGTCTGAAGCAGATATTGGAAGATTACAATATGATTCCAGATCCAATGTCCAATTTCTACGATGCACTTTGTGTTGGAATTGATTGGTTTGAAAATCTTAATACCGATGGCTTCTTTCAACTCTCTCCCGACAATTCTGAAGATGATGACATGGAACAACCCATTCCAGATTTCCAAAATGAGCCACCGGAGCAACCGGCCGAGCAGCCCCCAAAACGACCCTCCCTAGCTGCACAGCGACAGAGAACGGGAAGGATGACAGTGAACGACGTGTGGGAATATCTCCATCTTCCAATATCAGAAGCTTCGAAGAAACTGAATGTGTGCAATACAGTGTTGAAGAAAATATGCCGCCGGAGCGGCCTCAGCCGATGGCCTTACAGAAAGATACGGAGTTATGAGAGACGAATAGCGGCGCTGAGAGCTACAATGGATTCAAGCTATGGAGATACCAGAATCCGGGCTGAAGCCGAGATTGAAAGGGTTCAAAAAGAACTTGCTGATTTTTGTGCTCGAATCAGAATTTGA

Protein sequence

MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSNFYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAAQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIAALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI
Homology
BLAST of Lsi08G008400 vs. ExPASy Swiss-Prot
Match: Q9M1B0 (Protein NLP9 OS=Arabidopsis thaliana OX=3702 GN=NLP9 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.0e-07
Identity = 24/64 (37.50%), Postives = 39/64 (60.94%), Query Frame = 0

Query: 128 MTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIAALRATMD 187
           +++N + +Y    + +A+K L VC T LK+ICR+ G+ RWP RKI    R +  ++  +D
Sbjct: 536 VSLNVLQQYFSGSLKDAAKSLGVCPTTLKRICRQHGIMRWPSRKINKVNRSLRKIQTVLD 595

Query: 188 SSYG 192
           S  G
Sbjct: 596 SVQG 599

BLAST of Lsi08G008400 vs. ExPASy Swiss-Prot
Match: Q10S83 (Protein NLP1 OS=Oryza sativa subsp. japonica OX=39947 GN=NLP1 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 5.3e-07
Identity = 27/76 (35.53%), Postives = 47/76 (61.84%), Query Frame = 0

Query: 119 AAQRQRT---GRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSY 178
           A +++RT     +++ D+ ++    + EA+K L VC T LK+ICR+ G++RWP RKI+  
Sbjct: 611 AVEKRRTKTEKTVSLQDLRKHFAGSLKEAAKNLGVCPTTLKRICRQHGINRWPSRKIKKV 670

Query: 179 ERRIAALRATMDSSYG 192
              +  L+  +DS +G
Sbjct: 671 GHSLKKLQMVIDSVHG 686

BLAST of Lsi08G008400 vs. ExPASy Swiss-Prot
Match: Q84TH9 (Protein NLP7 OS=Arabidopsis thaliana OX=3702 GN=NLP7 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 6.9e-07
Identity = 24/57 (42.11%), Postives = 36/57 (63.16%), Query Frame = 0

Query: 135 EYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIAALRATMDSSYG 192
           +Y    + +A+K L VC T +K+ICR+ G+SRWP RKI+   R I  L+  ++S  G
Sbjct: 599 QYFTGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKIKKVNRSITKLKRVIESVQG 655

BLAST of Lsi08G008400 vs. ExPASy Swiss-Prot
Match: Q9LE38 (Protein NLP4 OS=Arabidopsis thaliana OX=3702 GN=NLP4 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.2e-06
Identity = 26/78 (33.33%), Postives = 47/78 (60.26%), Query Frame = 0

Query: 117 SLAAQRQRT---GRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIR 176
           S+ A+++RT     +T++ + +Y    + +A+K + VC T LK+ICR+ G+ RWP RKI+
Sbjct: 545 SMMAEKKRTKADKTITLDVLRQYFAGSLKDAAKNIGVCPTTLKRICRQHGIQRWPSRKIK 604

Query: 177 SYERRIAALRATMDSSYG 192
                +  ++  +DS  G
Sbjct: 605 KVGHSLQKIQRVIDSVQG 622

BLAST of Lsi08G008400 vs. ExPASy Swiss-Prot
Match: Q9FGD1 (Protein RKD3 OS=Arabidopsis thaliana OX=3702 GN=RKD3 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.2e-06
Identity = 44/161 (27.33%), Postives = 85/161 (52.80%), Query Frame = 0

Query: 64  ALCVGIDWFENLNTDGFFQLSPDNSE--------DDDMEQPIPDFQNEPPEQPAEQPPKR 123
           +L +   W+   N +   Q+ P+  E        D +MEQ + +  N   E+ ++   KR
Sbjct: 87  SLDISSGWWNENNGNYNNQVEPNLDEISRTNTMGDPNMEQILHEDVNTMKEKTSQ---KR 146

Query: 124 PSLAAQRQRTG---RMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKI 183
             +  + +  G    M+   + +Y ++PI++A+K+LN+  T+LKK CR  G+ RWP+RK+
Sbjct: 147 IIMKRRYREDGVINNMSREMMKQYFYMPITKAAKELNIGVTLLKKRCRELGIPRWPHRKL 206

Query: 184 RSYERRIAALRATMDSSYGDT---RIRAEAEIERVQKELAD 211
            S    IA L+  + ++ G T   ++R   E+  ++K++ +
Sbjct: 207 TSLNALIANLKDLLGNTKGRTPKSKLRNALELLEMEKKMIE 244

BLAST of Lsi08G008400 vs. ExPASy TrEMBL
Match: A0A5A7V999 (Protein RKD2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00220 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 7.9e-115
Identity = 207/217 (95.39%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MI HAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 154 MISHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 213

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNSED++MEQ  PD QNEPPEQP EQPP+RPSLAA
Sbjct: 214 FYDALCVGIDWFENLNTDGFFQLSPDNSEDEEMEQTTPDCQNEPPEQPEEQPPRRPSLAA 273

Query: 121 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 180
           QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA
Sbjct: 274 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 333

Query: 181 ALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           ALRATM+SSYGDTR+RAEAEIERVQKELADFCARIRI
Sbjct: 334 ALRATMNSSYGDTRVRAEAEIERVQKELADFCARIRI 370

BLAST of Lsi08G008400 vs. ExPASy TrEMBL
Match: A0A1S3BCG6 (protein RKD2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488516 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 7.9e-115
Identity = 207/217 (95.39%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MI HAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 154 MISHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 213

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNSED++MEQ  PD QNEPPEQP EQPP+RPSLAA
Sbjct: 214 FYDALCVGIDWFENLNTDGFFQLSPDNSEDEEMEQTTPDCQNEPPEQPEEQPPRRPSLAA 273

Query: 121 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 180
           QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA
Sbjct: 274 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 333

Query: 181 ALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           ALRATM+SSYGDTR+RAEAEIERVQKELADFCARIRI
Sbjct: 334 ALRATMNSSYGDTRVRAEAEIERVQKELADFCARIRI 370

BLAST of Lsi08G008400 vs. ExPASy TrEMBL
Match: A0A1S3BCY1 (uncharacterized protein LOC103488516 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488516 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 5.7e-113
Identity = 206/217 (94.93%), Postives = 211/217 (97.24%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MI HAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 154 MISHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 213

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNS D++MEQ  PD QNEPPEQP EQPP+RPSLAA
Sbjct: 214 FYDALCVGIDWFENLNTDGFFQLSPDNS-DEEMEQTTPDCQNEPPEQPEEQPPRRPSLAA 273

Query: 121 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 180
           QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA
Sbjct: 274 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 333

Query: 181 ALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           ALRATM+SSYGDTR+RAEAEIERVQKELADFCARIRI
Sbjct: 334 ALRATMNSSYGDTRVRAEAEIERVQKELADFCARIRI 369

BLAST of Lsi08G008400 vs. ExPASy TrEMBL
Match: A0A6J1BQU6 (protein RKD3-like OS=Momordica charantia OX=3673 GN=LOC111004772 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 6.5e-109
Identity = 198/217 (91.24%), Postives = 207/217 (95.39%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           +ICHAILEHKPIVNVDNI PQYQMFDFC+KS EEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 21  IICHAILEHKPIVNVDNIGPQYQMFDFCDKSSEEIKQFLLQYCLKQILEDYNMIPDPMSN 80

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNSED+DM+QP P+FQNEPP    E+PP+RPSLAA
Sbjct: 81  FYDALCVGIDWFENLNTDGFFQLSPDNSEDEDMDQPPPEFQNEPP----ERPPRRPSLAA 140

Query: 121 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 180
           QR RTGRMTVNDVW+YLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA
Sbjct: 141 QRARTGRMTVNDVWQYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 200

Query: 181 ALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           ALRATM+SSYGDTR RAEAEIERVQKELADFCA IRI
Sbjct: 201 ALRATMNSSYGDTRARAEAEIERVQKELADFCAGIRI 233

BLAST of Lsi08G008400 vs. ExPASy TrEMBL
Match: A0A6J1GK73 (uncharacterized protein LOC111454636 OS=Cucurbita moschata OX=3662 GN=LOC111454636 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.3e-105
Identity = 194/218 (88.99%), Postives = 205/218 (94.04%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MICHAILEHKPIVNVDNISPQYQMFDFC+KS EEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 154 MICHAILEHKPIVNVDNISPQYQMFDFCDKSFEEIKQFLLQYCLKQILEDYNMIPDPMSN 213

Query: 61  FYDALCVGIDWFENLN-TDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLA 120
           FYDALCVGIDWFENLN TD FFQ SPDNSED+DM+Q +P FQN+ PE P  QP +RPSLA
Sbjct: 214 FYDALCVGIDWFENLNTTDAFFQQSPDNSEDEDMDQSVPGFQNDTPE-PQGQPSRRPSLA 273

Query: 121 AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI 180
           AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI
Sbjct: 274 AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI 333

Query: 181 AALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           AAL+ T++SSYGDTR RAEAEI+RVQKEL+DFCARIRI
Sbjct: 334 AALKTTINSSYGDTRTRAEAEIQRVQKELSDFCARIRI 370

BLAST of Lsi08G008400 vs. NCBI nr
Match: XP_008445526.1 (PREDICTED: protein RKD2-like isoform X1 [Cucumis melo] >KAA0064713.1 protein RKD2-like isoform X1 [Cucumis melo var. makuwa] >TYK00732.1 protein RKD2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 422.9 bits (1086), Expect = 1.6e-114
Identity = 207/217 (95.39%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MI HAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 154 MISHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 213

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNSED++MEQ  PD QNEPPEQP EQPP+RPSLAA
Sbjct: 214 FYDALCVGIDWFENLNTDGFFQLSPDNSEDEEMEQTTPDCQNEPPEQPEEQPPRRPSLAA 273

Query: 121 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 180
           QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA
Sbjct: 274 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 333

Query: 181 ALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           ALRATM+SSYGDTR+RAEAEIERVQKELADFCARIRI
Sbjct: 334 ALRATMNSSYGDTRVRAEAEIERVQKELADFCARIRI 370

BLAST of Lsi08G008400 vs. NCBI nr
Match: KAE8647226.1 (hypothetical protein Csa_019049 [Cucumis sativus])

HSP 1 Score: 421.4 bits (1082), Expect = 4.7e-114
Identity = 208/218 (95.41%), Postives = 212/218 (97.25%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MI HAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 148 MISHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 207

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPP-KRPSLA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNSEDD+MEQP PD QNEPPEQP EQPP +RPSLA
Sbjct: 208 FYDALCVGIDWFENLNTDGFFQLSPDNSEDDEMEQPTPDCQNEPPEQPEEQPPRRRPSLA 267

Query: 121 AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI 180
           AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI
Sbjct: 268 AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI 327

Query: 181 AALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           AALRATM+SSYGDTR+RAEAEIERVQKEL DFCARIRI
Sbjct: 328 AALRATMNSSYGDTRVRAEAEIERVQKELTDFCARIRI 365

BLAST of Lsi08G008400 vs. NCBI nr
Match: XP_008445527.1 (PREDICTED: uncharacterized protein LOC103488516 isoform X2 [Cucumis melo])

HSP 1 Score: 416.8 bits (1070), Expect = 1.2e-112
Identity = 206/217 (94.93%), Postives = 211/217 (97.24%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MI HAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 154 MISHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 213

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNS D++MEQ  PD QNEPPEQP EQPP+RPSLAA
Sbjct: 214 FYDALCVGIDWFENLNTDGFFQLSPDNS-DEEMEQTTPDCQNEPPEQPEEQPPRRPSLAA 273

Query: 121 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 180
           QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA
Sbjct: 274 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 333

Query: 181 ALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           ALRATM+SSYGDTR+RAEAEIERVQKELADFCARIRI
Sbjct: 334 ALRATMNSSYGDTRVRAEAEIERVQKELADFCARIRI 369

BLAST of Lsi08G008400 vs. NCBI nr
Match: XP_022131644.1 (protein RKD3-like [Momordica charantia])

HSP 1 Score: 403.3 bits (1035), Expect = 1.3e-108
Identity = 198/217 (91.24%), Postives = 207/217 (95.39%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           +ICHAILEHKPIVNVDNI PQYQMFDFC+KS EEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 21  IICHAILEHKPIVNVDNIGPQYQMFDFCDKSSEEIKQFLLQYCLKQILEDYNMIPDPMSN 80

Query: 61  FYDALCVGIDWFENLNTDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLAA 120
           FYDALCVGIDWFENLNTDGFFQLSPDNSED+DM+QP P+FQNEPP    E+PP+RPSLAA
Sbjct: 81  FYDALCVGIDWFENLNTDGFFQLSPDNSEDEDMDQPPPEFQNEPP----ERPPRRPSLAA 140

Query: 121 QRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 180
           QR RTGRMTVNDVW+YLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA
Sbjct: 141 QRARTGRMTVNDVWQYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIA 200

Query: 181 ALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           ALRATM+SSYGDTR RAEAEIERVQKELADFCA IRI
Sbjct: 201 ALRATMNSSYGDTRARAEAEIERVQKELADFCAGIRI 233

BLAST of Lsi08G008400 vs. NCBI nr
Match: XP_023537902.1 (uncharacterized protein LOC111798799 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 396.4 bits (1017), Expect = 1.6e-106
Identity = 196/218 (89.91%), Postives = 207/218 (94.95%), Query Frame = 0

Query: 1   MICHAILEHKPIVNVDNISPQYQMFDFCEKSCEEIKQFLLQYCLKQILEDYNMIPDPMSN 60
           MICHAILEHKPIVNVDNISPQYQMFDFC+KS EEIKQFLLQYCLKQILEDYNMIPDPMSN
Sbjct: 154 MICHAILEHKPIVNVDNISPQYQMFDFCDKSFEEIKQFLLQYCLKQILEDYNMIPDPMSN 213

Query: 61  FYDALCVGIDWFENLN-TDGFFQLSPDNSEDDDMEQPIPDFQNEPPEQPAEQPPKRPSLA 120
           FYDALCVGIDWFENLN TD FFQ SPDNSED+DM+QP+P+FQN+ PE P  QP +RPSLA
Sbjct: 214 FYDALCVGIDWFENLNTTDAFFQQSPDNSEDEDMDQPVPEFQNDTPE-PQGQPSRRPSLA 273

Query: 121 AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI 180
           AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI
Sbjct: 274 AQRQRTGRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRI 333

Query: 181 AALRATMDSSYGDTRIRAEAEIERVQKELADFCARIRI 218
           AAL+ TM+SSYGDTR RAEAEI+RVQKEL+DFCARIRI
Sbjct: 334 AALKTTMNSSYGDTRARAEAEIQRVQKELSDFCARIRI 370

BLAST of Lsi08G008400 vs. TAIR 10
Match: AT3G59580.1 (Plant regulator RWP-RK family protein )

HSP 1 Score: 56.6 bits (135), Expect = 2.9e-08
Identity = 24/64 (37.50%), Postives = 39/64 (60.94%), Query Frame = 0

Query: 128 MTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIAALRATMD 187
           +++N + +Y    + +A+K L VC T LK+ICR+ G+ RWP RKI    R +  ++  +D
Sbjct: 536 VSLNVLQQYFSGSLKDAAKSLGVCPTTLKRICRQHGIMRWPSRKINKVNRSLRKIQTVLD 595

Query: 188 SSYG 192
           S  G
Sbjct: 596 SVQG 599

BLAST of Lsi08G008400 vs. TAIR 10
Match: AT3G59580.2 (Plant regulator RWP-RK family protein )

HSP 1 Score: 56.6 bits (135), Expect = 2.9e-08
Identity = 24/64 (37.50%), Postives = 39/64 (60.94%), Query Frame = 0

Query: 128 MTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIAALRATMD 187
           +++N + +Y    + +A+K L VC T LK+ICR+ G+ RWP RKI    R +  ++  +D
Sbjct: 536 VSLNVLQQYFSGSLKDAAKSLGVCPTTLKRICRQHGIMRWPSRKINKVNRSLRKIQTVLD 595

Query: 188 SSYG 192
           S  G
Sbjct: 596 SVQG 599

BLAST of Lsi08G008400 vs. TAIR 10
Match: AT4G24020.1 (NIN like protein 7 )

HSP 1 Score: 55.8 bits (133), Expect = 4.9e-08
Identity = 24/57 (42.11%), Postives = 36/57 (63.16%), Query Frame = 0

Query: 135 EYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIRSYERRIAALRATMDSSYG 192
           +Y    + +A+K L VC T +K+ICR+ G+SRWP RKI+   R I  L+  ++S  G
Sbjct: 599 QYFTGSLKDAAKSLGVCPTTMKRICRQHGISRWPSRKIKKVNRSITKLKRVIESVQG 655

BLAST of Lsi08G008400 vs. TAIR 10
Match: AT1G20640.1 (Plant regulator RWP-RK family protein )

HSP 1 Score: 55.1 bits (131), Expect = 8.3e-08
Identity = 26/78 (33.33%), Postives = 47/78 (60.26%), Query Frame = 0

Query: 117 SLAAQRQRT---GRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIR 176
           S+ A+++RT     +T++ + +Y    + +A+K + VC T LK+ICR+ G+ RWP RKI+
Sbjct: 545 SMMAEKKRTKADKTITLDVLRQYFAGSLKDAAKNIGVCPTTLKRICRQHGIQRWPSRKIK 604

Query: 177 SYERRIAALRATMDSSYG 192
                +  ++  +DS  G
Sbjct: 605 KVGHSLQKIQRVIDSVQG 622

BLAST of Lsi08G008400 vs. TAIR 10
Match: AT1G20640.2 (Plant regulator RWP-RK family protein )

HSP 1 Score: 55.1 bits (131), Expect = 8.3e-08
Identity = 26/78 (33.33%), Postives = 47/78 (60.26%), Query Frame = 0

Query: 117 SLAAQRQRT---GRMTVNDVWEYLHLPISEASKKLNVCNTVLKKICRRSGLSRWPYRKIR 176
           S+ A+++RT     +T++ + +Y    + +A+K + VC T LK+ICR+ G+ RWP RKI+
Sbjct: 545 SMMAEKKRTKADKTITLDVLRQYFAGSLKDAAKNIGVCPTTLKRICRQHGIQRWPSRKIK 604

Query: 177 SYERRIAALRATMDSSYG 192
                +  ++  +DS  G
Sbjct: 605 KVGHSLQKIQRVIDSVQG 622

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M1B04.0e-0737.50Protein NLP9 OS=Arabidopsis thaliana OX=3702 GN=NLP9 PE=2 SV=1[more]
Q10S835.3e-0735.53Protein NLP1 OS=Oryza sativa subsp. japonica OX=39947 GN=NLP1 PE=2 SV=1[more]
Q84TH96.9e-0742.11Protein NLP7 OS=Arabidopsis thaliana OX=3702 GN=NLP7 PE=1 SV=2[more]
Q9LE381.2e-0633.33Protein NLP4 OS=Arabidopsis thaliana OX=3702 GN=NLP4 PE=2 SV=1[more]
Q9FGD11.2e-0627.33Protein RKD3 OS=Arabidopsis thaliana OX=3702 GN=RKD3 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7V9997.9e-11595.39Protein RKD2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3BCG67.9e-11595.39protein RKD2-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488516 PE=4 SV=1[more]
A0A1S3BCY15.7e-11394.93uncharacterized protein LOC103488516 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1BQU66.5e-10991.24protein RKD3-like OS=Momordica charantia OX=3673 GN=LOC111004772 PE=4 SV=1[more]
A0A6J1GK734.3e-10588.99uncharacterized protein LOC111454636 OS=Cucurbita moschata OX=3662 GN=LOC1114546... [more]
Match NameE-valueIdentityDescription
XP_008445526.11.6e-11495.39PREDICTED: protein RKD2-like isoform X1 [Cucumis melo] >KAA0064713.1 protein RKD... [more]
KAE8647226.14.7e-11495.41hypothetical protein Csa_019049 [Cucumis sativus][more]
XP_008445527.11.2e-11294.93PREDICTED: uncharacterized protein LOC103488516 isoform X2 [Cucumis melo][more]
XP_022131644.11.3e-10891.24protein RKD3-like [Momordica charantia][more]
XP_023537902.11.6e-10689.91uncharacterized protein LOC111798799 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G59580.12.9e-0837.50Plant regulator RWP-RK family protein [more]
AT3G59580.22.9e-0837.50Plant regulator RWP-RK family protein [more]
AT4G24020.14.9e-0842.11NIN like protein 7 [more]
AT1G20640.18.3e-0833.33Plant regulator RWP-RK family protein [more]
AT1G20640.28.3e-0833.33Plant regulator RWP-RK family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 194..214
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 83..125
NoneNo IPR availablePANTHERPTHR46373:SF5RWP-RK DOMAIN PROTEINcoord: 1..214
IPR003035RWP-RK domainPFAMPF02042RWP-RKcoord: 128..174
e-value: 9.2E-20
score: 70.4
IPR003035RWP-RK domainPROSITEPS51519RWP_RKcoord: 115..195
score: 17.654749
IPR044607Transcription factor RKD-likePANTHERPTHR46373PROTEIN RKD4coord: 1..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G008400.1Lsi08G008400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity