Lsi08G008090 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G008090
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Neutral ceramidase
Location	chr08: 16456518 .. 16457354 (+)
RNA-Seq Expression	Lsi08G008090
Synteny	Lsi08G008090

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGGCGGCAGTTGGTGAAGCTCATGGCTATGGCCATTGTAGTTGCAGCTGTGAGTTGGTGTGTTAATGGAGATTGGTTGGTCGGCGTTGGGAGCTTCGACATGACTGGCCCGGCTGCCGGAGTGAACATGATGGGGTATGCGAATATGGATCAGAACACGGCGGGGATTCATTTTAGGCTGAGAGCGAGGAGTTTCGTTGTTGCTAAAAGTGTTGATGGGCCTAGAATTGCGTTTGTTAATTTGGATGCTGGAATGGCTTCTCAATTGGTCACCATTAAAGTGCTTGAGAGGCTCAAATCTAGGTATATTAATATGCATATTAATAATAGGAGCTTCGTCAAATATTTATATACAATGAACTAACAAAGACATTTTTTTAAAAAAAAAGAGAGAAATATTTCAATGTCTAGTTTGTCCTAAAATTTTGACTTTGTTTCGGATTGGTGATCGAGTAAAAAAGAAAAAGAAAAAGAAAAATCAAGTTCATATAATTTAAAAAAATACATATTGGCATAATCTAATTTGTGTACATGTTAAATGTGATGTCATTATTTTTCCTTTAAAATTTCAAGGGGAAATTAGCAAACTAAAAATGAGAACATTTTTGTGCGAAGGTTTGGGGATTTATACACAGAAGAGAACGTAGCAATAAGTGGAATCCACACACACGCCGGCCCCGGCGGCTATCTACAATACTTGGTCTACTCCATAACATCTCTCGGCTTCGTCCAACAATCGCTCGACGCCATCGTCGACGCCATTGAACAGAGCATCGTACAAGCTCACCAAAGCCTCAAATCAGGCTTCATCTTATTCAACAAAGGCAAGCAATAA

mRNA sequence

ATGCGGCGGCAGTTGGTGAAGCTCATGGCTATGGCCATTGTAGTTGCAGCTGTGAGTTGGTGTGTTAATGGAGATTGGTTGGTCGGCGTTGGGAGCTTCGACATGACTGGCCCGGCTGCCGGAGTGAACATGATGGGGTATGCGAATATGGATCAGAACACGGCGGGGATTCATTTTAGGCTGAGAGCGAGGAGTTTCGTTGTTGCTAAAAGTGTTGATGGGCCTAGAATTGCGTTTGTTAATTTGGATGCTGGAATGGCTTCTCAATTGGTCACCATTAAAGTGCTTGAGAGGCTCAAATCTAGGTTTGGGGATTTATACACAGAAGAGAACGTAGCAATAAGTGGAATCCACACACACGCCGGCCCCGGCGGCTATCTACAATACTTGGTCTACTCCATAACATCTCTCGGCTTCGTCCAACAATCGCTCGACGCCATCGTCGACGCCATTGAACAGAGCATCGTACAAGCTCACCAAAGCCTCAAATCAGGCTTCATCTTATTCAACAAAGGCAAGCAATAA

Coding sequence (CDS)

ATGCGGCGGCAGTTGGTGAAGCTCATGGCTATGGCCATTGTAGTTGCAGCTGTGAGTTGGTGTGTTAATGGAGATTGGTTGGTCGGCGTTGGGAGCTTCGACATGACTGGCCCGGCTGCCGGAGTGAACATGATGGGGTATGCGAATATGGATCAGAACACGGCGGGGATTCATTTTAGGCTGAGAGCGAGGAGTTTCGTTGTTGCTAAAAGTGTTGATGGGCCTAGAATTGCGTTTGTTAATTTGGATGCTGGAATGGCTTCTCAATTGGTCACCATTAAAGTGCTTGAGAGGCTCAAATCTAGGTTTGGGGATTTATACACAGAAGAGAACGTAGCAATAAGTGGAATCCACACACACGCCGGCCCCGGCGGCTATCTACAATACTTGGTCTACTCCATAACATCTCTCGGCTTCGTCCAACAATCGCTCGACGCCATCGTCGACGCCATTGAACAGAGCATCGTACAAGCTCACCAAAGCCTCAAATCAGGCTTCATCTTATTCAACAAAGGCAAGCAATAA

Protein sequence

MRRQLVKLMAMAIVVAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKGKQ

Homology

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BLAST of Lsi08G008090 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.2e-57
Identity = 108/151 (71.52%), Postives = 133/151 (88.08%), Query Frame = 0


Query: 23  NGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNL 82
           + D+L+G+GS+D+TGPAA VNMMGYANM+Q  +G+HFRLRAR+F+VA+     RIAFVNL
Sbjct: 26  DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIAFVNL 85

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DAGMASQLVTIKV+ERLK R+G+LYTEENVAISG HTHAGPGGYLQY++Y +TSLGFV Q
Sbjct: 86  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 145

Query: 143 SLDAIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           S +A+VD IEQSI+QAH++L+ G IL NKG+
Sbjct: 146 SFNALVDGIEQSIIQAHENLRPGSILINKGE 175

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BLAST of Lsi08G008090 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 1.9e-55
Identity = 106/147 (72.11%), Postives = 127/147 (86.39%), Query Frame = 0


Query: 26  WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAG 85
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +GIHFRLRAR+F+VA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 86  MASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSLD 145
           MASQ+VTIKVLERLK+R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QS D
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 146 AIVDAIEQSIVQAHQSLKSGFILFNKG 173
            +V+ IEQSIVQAH+SL+ G    NKG
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKG 171

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BLAST of Lsi08G008090 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 5.4e-55
Identity = 101/151 (66.89%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 23  NGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNL 82
           + ++L+G+GS+D+TGPAA VNMMGYANM+Q  +GIHFRLRAR+F+V++   G R+ FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNL 93

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DA MASQ+V +KV+ERLK+R+GDLYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 143 SLDAIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           S DA+VD IE SI+QAH++L+ G I  N G+
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGE 183

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BLAST of Lsi08G008090 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.6e-54
Identity = 108/165 (65.45%), Postives = 136/165 (82.42%), Query Frame = 0


Query: 9   MAMAIVVAAVSWCVNGD-WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFV 68
           + +A+V+   S  ++   +LVG+GSFD+TGPAA VNMMGYAN +Q  +GIHFRL++R+F+
Sbjct: 23  LLLALVLLNCSLVLSASPYLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLKSRAFI 82

Query: 69  VAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYL 128
           VA+  +G R+ FVN+DA MASQ+VTIKVLERLK+R+GDLY E NVAISGIHTHAGPGGYL
Sbjct: 83  VAEP-NGKRVVFVNIDACMASQIVTIKVLERLKARYGDLYNENNVAISGIHTHAGPGGYL 142

Query: 129 QYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKG 173
           QY+VY +TSLGFV+QS D IVD IEQSIV+AH +L+ G I  NKG
Sbjct: 143 QYVVYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKG 186

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BLAST of Lsi08G008090 vs. ExPASy Swiss-Prot
Match: Q5W7F1 (Neutral ceramidase OS=Danio rerio OX=7955 GN=asah2 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.1e-34
Identity = 71/148 (47.97%), Postives = 100/148 (67.57%), Query Frame = 0


Query: 26  WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAG 85
           +L+GVG  D TGP A + MMGYAN DQ   G+H RL +R+F+V       R+ FV  D G
Sbjct: 61  YLIGVGRADCTGPVADLPMMGYANTDQTARGLHTRLFSRAFIVDDG--NKRVVFVTSDIG 120

Query: 86  MASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSLD 145
           M SQ + ++V + LK ++GDLY ++NV +SG HTH+G GGY QY ++ ITS G+++ S+ 
Sbjct: 121 MVSQRLRLEVFQALKEKYGDLYRQDNVVLSGTHTHSGVGGYFQYTLFMITSKGYIKPSIQ 180

Query: 146 AIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           AIV  I +SI  AH++L+ G I  NKG+
Sbjct: 181 AIVSGIVKSIDIAHRNLRPGRIFINKGQ 206

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BLAST of Lsi08G008090 vs. ExPASy TrEMBL
Match: A0A1S3CMI6 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.8e-75
Identity = 149/172 (86.63%), Postives = 157/172 (91.28%), Query Frame = 0


Query: 11  MAIVVAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 70
           MAIVV  VSWCVNGDWLVGVGSFDMTGPAA VNMMGYANMDQ TAGIHFRLRAR+FVVA+
Sbjct: 1   MAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVAR 60

Query: 71  SVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------RFGDLYTEENVAISGIHTHA 130
           S+DGPRIAFVNLDAGMASQLVTIKVLERL+S         RFGDLYTEENVAISGIHTHA
Sbjct: 61  SIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHA 120

Query: 131 GPGGYLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           GPGGYLQYLVYSITSLGFVQQS DAIV+AIEQSI+QAH+SLK GFILFNKG+
Sbjct: 121 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGE 172

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BLAST of Lsi08G008090 vs. ExPASy TrEMBL
Match: A0A5N6RC17 (Neutral ceramidase OS=Carpinus fangiana OX=176857 GN=FH972_014039 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.6e-65
Identity = 127/164 (77.44%), Postives = 143/164 (87.20%), Query Frame = 0


Query: 8   LMAMAIVVAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFV 67
           ++ + +        +NG++L+GVGS+DMTGPAAGVNMMGYAN+DQ TAGIHF LRAR+FV
Sbjct: 16  VIVLCVFCGVFGAAINGEYLIGVGSYDMTGPAAGVNMMGYANIDQTTAGIHFWLRARAFV 75

Query: 68  VAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYL 127
           VA+S  GPR AFVNLDAGMASQLVTIKV+ERLKSRFGDLYTEENVAISGIHTHAGPGGYL
Sbjct: 76  VAESSQGPRFAFVNLDAGMASQLVTIKVVERLKSRFGDLYTEENVAISGIHTHAGPGGYL 135

Query: 128 QYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNK 172
           QYLVYS+TSLGFVQQS DAIVDAIE SIVQAH +LKSG +  NK
Sbjct: 136 QYLVYSVTSLGFVQQSFDAIVDAIEHSIVQAHNNLKSGSVFINK 179

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BLAST of Lsi08G008090 vs. ExPASy TrEMBL
Match: A0A6J5W870 (Neutral ceramidase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7600 PE=3 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 6.0e-65
Identity = 131/169 (77.51%), Postives = 146/169 (86.39%), Query Frame = 0


Query: 5   LVKLMAMAIV-VAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRA 64
           L  L+ M IV V  +  C    +L+GVGS+DMTGPAAGVNMMGYANMDQ+TAG+HFRLRA
Sbjct: 15  LSSLIIMIIVGVGVIRRCNGEGYLIGVGSYDMTGPAAGVNMMGYANMDQSTAGVHFRLRA 74

Query: 65  RSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGP 124
           R+F+VA+S  GPR AFVNLDAGMASQLV IKVL++LKSRFGDLYTEENVAISGIHTHAGP
Sbjct: 75  RTFIVAESSQGPRFAFVNLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAISGIHTHAGP 134

Query: 125 GGYLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKG 173
           GGYLQYLVYSITSLGFVQQS DAIV+AIEQSIVQAH +LK G +  NKG
Sbjct: 135 GGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHHNLKPGSVFINKG 183

[Close]

BLAST of Lsi08G008090 vs. ExPASy TrEMBL
Match: A0A6P5S6T0 (Neutral ceramidase OS=Prunus avium OX=42229 GN=LOC110753614 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 7.8e-65
Identity = 130/169 (76.92%), Postives = 146/169 (86.39%), Query Frame = 0


Query: 5   LVKLMAMAIV-VAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRA 64
           L  L+ M IV +  +  C    +L+GVGS+DMTGPAAGVNMMGYANMDQ+TAG+HFRLRA
Sbjct: 15  LSSLIIMIIVGIGVIRRCNGEGYLIGVGSYDMTGPAAGVNMMGYANMDQSTAGVHFRLRA 74

Query: 65  RSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGP 124
           R+F+VA+S  GPR AFVNLDAGMASQLV IKVL++LKSRFGDLYTEENVAISGIHTHAGP
Sbjct: 75  RTFIVAESSQGPRFAFVNLDAGMASQLVNIKVLDKLKSRFGDLYTEENVAISGIHTHAGP 134

Query: 125 GGYLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKG 173
           GGYLQYLVYSITSLGFVQQS DAIV+AIEQSIVQAH +LK G +  NKG
Sbjct: 135 GGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHHNLKPGSVFINKG 183

[Close]

BLAST of Lsi08G008090 vs. ExPASy TrEMBL
Match: A0A7N2KSZ7 (Neutral ceramidase OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.0e-64
Identity = 130/167 (77.84%), Postives = 144/167 (86.23%), Query Frame = 0


Query: 6   VKLMAMAIVVAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARS 65
           V L+ +  ++  V    NGD+L+GVGSFD+TGPAAGV MMGYANMDQ+TAGIHFR+RAR+
Sbjct: 8   VSLVIVISIICGVLRATNGDYLIGVGSFDVTGPAAGVKMMGYANMDQSTAGIHFRIRART 67

Query: 66  FVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGG 125
           FVVA+S  GPR AFVNLDAGMASQLVTIKVLE+LKSRFGDLYTEENVAISGI THAGPGG
Sbjct: 68  FVVAESSQGPRFAFVNLDAGMASQLVTIKVLEKLKSRFGDLYTEENVAISGIDTHAGPGG 127

Query: 126 YLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKG 173
           YLQYLVYSITSLGFV QS DAIV+AIE SIVQAH +LK G I  NKG
Sbjct: 128 YLQYLVYSITSLGFVHQSFDAIVNAIELSIVQAHNNLKPGSIFINKG 174

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BLAST of Lsi08G008090 vs. NCBI nr
Match: XP_038885883.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 306.6 bits (784), Expect = 1.4e-79
Identity = 151/162 (93.21%), Postives = 159/162 (98.15%), Query Frame = 0


Query: 11  MAIVVAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 70
           MA V+AAVSWCVNGDWLVGVGSFDMTGPAAGVNM+GYANMDQNTAGIHFRLRAR+FVVAK
Sbjct: 1   MATVIAAVSWCVNGDWLVGVGSFDMTGPAAGVNMIGYANMDQNTAGIHFRLRARTFVVAK 60

Query: 71  SVDGPRIAFVNLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYL 130
           +VDGPRIAF+NLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYL
Sbjct: 61  TVDGPRIAFINLDAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYL 120

Query: 131 VYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKG 173
           VYSITSLGFVQQS DAIV+AIEQSI+QAH+SLK GFILFNKG
Sbjct: 121 VYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKG 162

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BLAST of Lsi08G008090 vs. NCBI nr
Match: XP_031742885.1 (neutral ceramidase 2 [Cucumis sativus])

HSP 1 Score: 296.6 bits (758), Expect = 1.4e-76
Identity = 151/168 (89.88%), Postives = 157/168 (93.45%), Query Frame = 0


Query: 11  MAIVVAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 70
           MA VV AVSWCVNGDWLVGVGSFDMTGPAA VNMMGYANMDQNTAGIHFRLRAR+FVVA 
Sbjct: 1   MATVVVAVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVAS 60

Query: 71  SVDGPRIAFVNLDAGMASQLVTIKVLERLKS-----RFGDLYTEENVAISGIHTHAGPGG 130
           SVDGPRIAFVNLDAGMASQLVTIKVLERLKS     RFGD+YTEENVAISGIHTHAGPGG
Sbjct: 61  SVDGPRIAFVNLDAGMASQLVTIKVLERLKSSILVQRFGDVYTEENVAISGIHTHAGPGG 120

Query: 131 YLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           YLQYLVYSITSLGFVQQS DAIV+AIEQSI+QAH+SLK GFILFNKG+
Sbjct: 121 YLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGE 168

[Close]

BLAST of Lsi08G008090 vs. NCBI nr
Match: XP_008464893.1 (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 290.8 bits (743), Expect = 7.7e-75
Identity = 149/172 (86.63%), Postives = 157/172 (91.28%), Query Frame = 0


Query: 11  MAIVVAAVSWCVNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAK 70
           MAIVV  VSWCVNGDWLVGVGSFDMTGPAA VNMMGYANMDQ TAGIHFRLRAR+FVVA+
Sbjct: 1   MAIVVLDVSWCVNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVAR 60

Query: 71  SVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------RFGDLYTEENVAISGIHTHA 130
           S+DGPRIAFVNLDAGMASQLVTIKVLERL+S         RFGDLYTEENVAISGIHTHA
Sbjct: 61  SIDGPRIAFVNLDAGMASQLVTIKVLERLQSRRNINISVQRFGDLYTEENVAISGIHTHA 120

Query: 131 GPGGYLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           GPGGYLQYLVYSITSLGFVQQS DAIV+AIEQSI+QAH+SLK GFILFNKG+
Sbjct: 121 GPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGE 172

[Close]

BLAST of Lsi08G008090 vs. NCBI nr
Match: KAG6599114.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 277.3 bits (708), Expect = 8.9e-71
Identity = 150/190 (78.95%), Postives = 163/190 (85.79%), Query Frame = 0


Query: 1   MRRQLVKLMAMAIVVAAVSWC--VNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIH 60
           MRRQL+K++A A V+AAVS C   +GDWLVGVGSFDMTGPAA VNMMGYANMDQ+ AGIH
Sbjct: 1   MRRQLLKVVATA-VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIH 60

Query: 61  FRLRARSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------------RF 120
           FRLRAR+F+VAKSVDGPRIAFVNLDAGMASQLVTIK+LERLK+               RF
Sbjct: 61  FRLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTYLSKLKPRHFIQRF 120

Query: 121 GDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLK 174
           G+LYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQS DAIV+AIEQSIVQAH SLK
Sbjct: 121 GNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLK 180

[Close]

BLAST of Lsi08G008090 vs. NCBI nr
Match: KAG7030054.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 277.3 bits (708), Expect = 8.9e-71
Identity = 150/190 (78.95%), Postives = 163/190 (85.79%), Query Frame = 0


Query: 1   MRRQLVKLMAMAIVVAAVSWC--VNGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIH 60
           MRRQL+K++A A V+AAVS C   +GDWLVGVGSFDMTGPAA VNMMGYANMDQ+ AGIH
Sbjct: 1   MRRQLLKVVATA-VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIH 60

Query: 61  FRLRARSFVVAKSVDGPRIAFVNLDAGMASQLVTIKVLERLKS---------------RF 120
           FRLRAR+F+VAKSVDGPRIAFVNLDAGMASQLVTIK+LERLK+               RF
Sbjct: 61  FRLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRF 120

Query: 121 GDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSLDAIVDAIEQSIVQAHQSLK 174
           G+LYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQS DAIV+AIEQSIVQAH SLK
Sbjct: 121 GNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLK 180

[Close]

BLAST of Lsi08G008090 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 224.2 bits (570), Expect = 8.3e-59
Identity = 108/151 (71.52%), Postives = 133/151 (88.08%), Query Frame = 0


Query: 23  NGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNL 82
           + D+L+G+GS+D+TGPAA VNMMGYANM+Q  +G+HFRLRAR+F+VA+     RIAFVNL
Sbjct: 26  DSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIAFVNL 85

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DAGMASQLVTIKV+ERLK R+G+LYTEENVAISG HTHAGPGGYLQY++Y +TSLGFV Q
Sbjct: 86  DAGMASQLVTIKVIERLKQRYGELYTEENVAISGTHTHAGPGGYLQYILYLVTSLGFVHQ 145

Query: 143 SLDAIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           S +A+VD IEQSI+QAH++L+ G IL NKG+
Sbjct: 146 SFNALVDGIEQSIIQAHENLRPGSILINKGE 175

[Close]

BLAST of Lsi08G008090 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 216.9 bits (551), Expect = 1.3e-56
Identity = 106/147 (72.11%), Postives = 127/147 (86.39%), Query Frame = 0


Query: 26  WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAG 85
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +GIHFRLRAR+F+VA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 86  MASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSLD 145
           MASQ+VTIKVLERLK+R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QS D
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 146 AIVDAIEQSIVQAHQSLKSGFILFNKG 173
            +V+ IEQSIVQAH+SL+ G    NKG
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKG 171

[Close]

BLAST of Lsi08G008090 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 216.9 bits (551), Expect = 1.3e-56
Identity = 106/147 (72.11%), Postives = 127/147 (86.39%), Query Frame = 0


Query: 26  WLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNLDAG 85
           +L+GVGS+D+TGPAA VNMMGYAN DQ  +GIHFRLRAR+F+VA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 86  MASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSLD 145
           MASQ+VTIKVLERLK+R+G+LYTE+NVAISGIHTHAGPGGYLQY+ Y +TSLGFV+QS D
Sbjct: 86  MASQIVTIKVLERLKARYGELYTEKNVAISGIHTHAGPGGYLQYVTYIVTSLGFVRQSFD 145

Query: 146 AIVDAIEQSIVQAHQSLKSGFILFNKG 173
            +V+ IEQSIVQAH+SL+ G    NKG
Sbjct: 146 VVVNGIEQSIVQAHESLRPGSAFVNKG 171

[Close]

BLAST of Lsi08G008090 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 215.3 bits (547), Expect = 3.9e-56
Identity = 101/151 (66.89%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 23  NGDWLVGVGSFDMTGPAAGVNMMGYANMDQNTAGIHFRLRARSFVVAKSVDGPRIAFVNL 82
           + ++L+G+GS+D+TGPAA VNMMGYANM+Q  +GIHFRLRAR+F+V++   G R+ FVNL
Sbjct: 34  HSEYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNL 93

Query: 83  DAGMASQLVTIKVLERLKSRFGDLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQ 142
           DA MASQ+V +KV+ERLK+R+GDLYTE+NV ISGIHTHAGPGGYLQY+VY +TSLGFV+Q
Sbjct: 94  DACMASQIVKLKVIERLKARYGDLYTEQNVGISGIHTHAGPGGYLQYVVYIVTSLGFVRQ 153

Query: 143 SLDAIVDAIEQSIVQAHQSLKSGFILFNKGK 174
           S DA+VD IE SI+QAH++L+ G I  N G+
Sbjct: 154 SFDALVDGIENSIIQAHENLRPGSIFLNNGE 183

The following BLAST results are available for this feature:

BLAST of Lsi08G008090 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KHQ8	1.2e-57	71.52	Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1	[more]
Q304B9	1.9e-55	72.11	Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1	[more]
F4HQM3	5.4e-55	66.89	Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1	[more]
Q0JL46	1.6e-54	65.45	Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1...	[more]
Q5W7F1	3.1e-34	47.97	Neutral ceramidase OS=Danio rerio OX=7955 GN=asah2 PE=1 SV=1	[more]

BLAST of Lsi08G008090 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CMI6	3.8e-75	86.63	Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1	[more]
A0A5N6RC17	4.6e-65	77.44	Neutral ceramidase OS=Carpinus fangiana OX=176857 GN=FH972_014039 PE=3 SV=1	[more]
A0A6J5W870	6.0e-65	77.51	Neutral ceramidase OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS7600 PE=3 SV=1	[more]
A0A6P5S6T0	7.8e-65	76.92	Neutral ceramidase OS=Prunus avium OX=42229 GN=LOC110753614 PE=3 SV=1	[more]
A0A7N2KSZ7	1.0e-64	77.84	Neutral ceramidase OS=Quercus lobata OX=97700 PE=3 SV=1	[more]

BLAST of Lsi08G008090 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885883.1	1.4e-79	93.21	neutral ceramidase 2-like [Benincasa hispida]	[more]
XP_031742885.1	1.4e-76	89.88	neutral ceramidase 2 [Cucumis sativus]	[more]
XP_008464893.1	7.7e-75	86.63	PREDICTED: neutral ceramidase-like [Cucumis melo]	[more]
KAG6599114.1	8.9e-71	78.95	Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7030054.1	8.9e-71	78.95	Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lsi08G008090 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58980.1	8.3e-59	71.52	Neutral/alkaline non-lysosomal ceramidase	[more]
AT2G38010.1	1.3e-56	72.11	Neutral/alkaline non-lysosomal ceramidase	[more]
AT2G38010.2	1.3e-56	72.11	Neutral/alkaline non-lysosomal ceramidase	[more]
AT1G07380.1	3.9e-56	66.89	Neutral/alkaline non-lysosomal ceramidase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031329	Neutral/alkaline non-lysosomal ceramidase, N-terminal	PFAM	PF04734	Ceramidase_alk	coord: 26..173 e-value: 1.2E-60 score: 205.5
IPR006823	Neutral/alkaline nonlysosomal ceramidase	PANTHER	PTHR12670	CERAMIDASE	coord: 9..173
None	No IPR available	PANTHER	PTHR12670:SF17	NEUTRAL CERAMIDASE 2	coord: 9..173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G008090.1	Lsi08G008090.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046514	ceramide catabolic process
biological_process	GO:0042759	long-chain fatty acid biosynthetic process
biological_process	GO:0046512	sphingosine biosynthetic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0102121	ceramidase activity
molecular_function	GO:0017040	N-acylsphingosine amidohydrolase activity