Lsi08G007410 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G007410
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Protein kinase 2B
Location	chr08: 15758866 .. 15760107 (-)
RNA-Seq Expression	Lsi08G007410
Synteny	Lsi08G007410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAGAATTGAGATGGTTAGTGTGAAGGTTATAACAAAATTCCTTTATTGGTTCCTTGATTCCTTTAGAAGGAAAGGTCCCTGACTAAATTTCTGATAGTTTTTATATGGAAACATAAGAATTAACATCTTATGTCCATTCCTTTAAAAAAAAAATTATTCTCCCTAGTTTTAATAGGCGTTCATGGGTTATGTGATATATCTATGTTTCTTTAATGGGTTCAGGTTAGTTTTTGTGTATGATTGAATCTACTTGGTCTCGATATCAACTCTAAAAAGAGTGAGTAAACATTGGAGAAACTATGAACTTGTCAGAAAATGTGCACACAGAGAATACTAGCTTTTACATTATACATTATACAGTATTCATGTGACATATCATTGAGGTTTCTGATTTATGCTGTAATTTTATTTTTCATGAAAAAAAAATTCATGGCAATTAACCGTTTAAACTGCAACATTCACTTCTGTCTAGGTCGATTGACATCCAAAAGCGATGTTTATAGTTTTGGAGTTGTGTTGCTCGAATTGTTGTCGGGTCAACGTGCAGAGGACAATCTTGTAGATTGGGCAAGGCCATATTTGGGTGACAAAAGAAGGTTATTCCGAATAATGGACACCAAACTTGAAGGACGATATTCTCAAAAGGGGGCTTACGTAGCTGCAAATTTAGCTTCACAATGCCTTAGGAGTGAACCGCGAGCCCGACCTCGAATGGCAGAAATTTTGGGTGCATTGGAGGAACTTGAATCCCCCAAAACTCCAACTAGAATCTCGCATTCAGTTCAACGTACTACTCCTACTTTGTCAAACTCGAACGTCGATGGATCTATTTTGACATCGCATCAGAAGTCACCACAACGGTAATAACGAAGATGTATAGTATAGAGCATTGTAAGATTTGAGCTGGGTAAAATCTCTCATAACGTCCATGCTAGATGCTATTGCCAAATATTGATAGAATTTTTTGTGTATATTAAGGTTTTAGTCAAAGTAAAAATAGAAGAAATTGAATAGTTTCTCAAGTAATTATTTCTTGCAGTTTGATTGTTACTACAAAGTTACTTGTAGTGGTAGTAAGGTAAAATTGGCATTATGCCCTTTTAGCTAGCTCTCTCTTACCCGATTTATTTATTATTATCTTTTTAAGTATAAATGGGGTGGGAGAATTTAAACTACGAATCTCTTGGTCTTCCGCACATGCTGATACCAGTTGAGCTAAACTCTTTTTGATACTCTT

mRNA sequence

ATGCCGAGAATTGAGATGGTTAGTGTGAAGGTTATAACAAAATTCCTTTATTGGTTCCTTGATTCCTTTAGAAGGAAAGGTCGATTGACATCCAAAAGCGATGTTTATAGTTTTGGAGTTGTGTTGCTCGAATTGTTGTCGGGTCAACGTGCAGAGGACAATCTTGTAGATTGGGCAAGGCCATATTTGGGTGACAAAAGAAGGTTATTCCGAATAATGGACACCAAACTTGAAGGACGATATTCTCAAAAGGGGGCTTACGTAGCTGCAAATTTAGCTTCACAATGCCTTAGGAGTGAACCGCGAGCCCGACCTCGAATGGCAGAAATTTTGGGTGCATTGGAGGAACTTGAATCCCCCAAAACTCCAACTAGAATCTCGCATTCAGTTCAACGTACTACTCCTACTTTGTCAAACTCGAACGTCGATGGATCTATTTTGACATCGCATCAGAAGTCACCACAACGGTAATAACGAAGATGTATAGTATAGAGCATTGTAAGATTTGAGCTGGGTAAAATCTCTCATAACGTCCATGCTAGATGCTATTGCCAAATATTGATAGAATTTTTTGTGTATATTAAGGTTTTAGTCAAAGTAAAAATAGAAGAAATTGAATAGTTTCTCAAGTAATTATTTCTTGCAGTTTGATTGTTACTACAAAGTTACTTGTAGTGGTAGTAAGGTAAAATTGGCATTATGCCCTTTTAGCTAGCTCTCTCTTACCCGATTTATTTATTATTATCTTTTTAAGTATAAATGGGGTGGGAGAATTTAAACTACGAATCTCTTGGTCTTCCGCACATGCTGATACCAGTTGAGCTAAACTCTTTTTGATACTCTT

Coding sequence (CDS)

ATGCCGAGAATTGAGATGGTTAGTGTGAAGGTTATAACAAAATTCCTTTATTGGTTCCTTGATTCCTTTAGAAGGAAAGGTCGATTGACATCCAAAAGCGATGTTTATAGTTTTGGAGTTGTGTTGCTCGAATTGTTGTCGGGTCAACGTGCAGAGGACAATCTTGTAGATTGGGCAAGGCCATATTTGGGTGACAAAAGAAGGTTATTCCGAATAATGGACACCAAACTTGAAGGACGATATTCTCAAAAGGGGGCTTACGTAGCTGCAAATTTAGCTTCACAATGCCTTAGGAGTGAACCGCGAGCCCGACCTCGAATGGCAGAAATTTTGGGTGCATTGGAGGAACTTGAATCCCCCAAAACTCCAACTAGAATCTCGCATTCAGTTCAACGTACTACTCCTACTTTGTCAAACTCGAACGTCGATGGATCTATTTTGACATCGCATCAGAAGTCACCACAACGGTAA

Protein sequence

MPRIEMVSVKVITKFLYWFLDSFRRKGRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSILTSHQKSPQR

Homology

[Close]

BLAST of Lsi08G007410 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.2e-32
Identity = 81/139 (58.27%), Postives = 100/139 (71.94%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRA--------EDNLVDWARPYLGDKRRLFRIMDTKLE 86
           GRLT+KSDVYSFGVVLLELLSG+RA        E +LVDWA PYLGDKR+LFRIMDT+L 
Sbjct: 268 GRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLG 327

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRI-SHSVQRTTPTL 146
           G+Y QKGAY AA+LA QCL  + + RP+M+E+L  L++LES K  T + +   Q  +P  
Sbjct: 328 GQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPRG 387

Query: 147 SNSNVDGSILTSHQKSPQR 157
           SN    GSI+   QKSP+R
Sbjct: 388 SN----GSIV---QKSPRR 399

[Close]

BLAST of Lsi08G007410 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 5.8e-32
Identity = 85/158 (53.80%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 7   VSVKVITKFLYWFLDSFRRKGRLTSKSDVYSFGVVLLELLSGQRAEDN--------LVDW 66
           VS KVI    Y     +   GRLT+KSDVYSFGVVLLEL+SG+RA DN        LVDW
Sbjct: 252 VSTKVIGTHGY-AAPEYVATGRLTAKSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDW 311

Query: 67  ARPYLGDKRRLFRIMDTKLEGRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELE 126
           A PYLGDKR+LFRIMDTKL G+Y QKGA+ AANLA QCL  + + RP+M+E+L  LE+LE
Sbjct: 312 ATPYLGDKRKLFRIMDTKLGGQYPQKGAFTAANLALQCLNPDAKLRPKMSEVLVTLEQLE 371

Query: 127 SPKTPTRISHSVQRTTPTLSNSNVDGSILTSHQKSPQR 157
           S   P   +   Q  +P   +S+V        QKSP R
Sbjct: 372 SVAKPG--TKHTQMESPRFHHSSV-------MQKSPVR 399

[Close]

BLAST of Lsi08G007410 vs. ExPASy Swiss-Prot
Match: Q5PP29 (Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN=PBL4 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 3.2e-30
Identity = 70/121 (57.85%), Postives = 86/121 (71.07%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAED--------NLVDWARPYLGDKRRLFRIMDTKLE 86
           GR+T+KSDVYSFGVVLLELLSG+   D        NLVDWA PYLGDKR++FRIMDTKL 
Sbjct: 267 GRITAKSDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTKLG 326

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLS 140
           G+Y  KGA + AN A QCL  EP+ RP+M+++L  LEELE       IS+SV + T + S
Sbjct: 327 GQYPHKGACLTANTALQCLNQEPKLRPKMSDVLSTLEELEMTLKSGSISNSVMKLTSSSS 386

[Close]

BLAST of Lsi08G007410 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.5e-27
Identity = 63/101 (62.38%), Postives = 77/101 (76.24%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQ--------RAEDNLVDWARPYLGDKRRLFRIMDTKLE 86
           GRLTSKSDVYSFGVVLLELLSG+          E NLVDWA PYL D+R++FRIMDTKL 
Sbjct: 267 GRLTSKSDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTKLG 326

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELES 120
           G+Y  KGA  AAN+A +CL +EP+ RP MA++L  L++LE+
Sbjct: 327 GQYPHKGACAAANIALRCLNTEPKLRPDMADVLSTLQQLET 367

[Close]

BLAST of Lsi08G007410 vs. ExPASy Swiss-Prot
Match: F4J0D2 (Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 PE=1 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 2.0e-24
Identity = 63/139 (45.32%), Postives = 85/139 (61.15%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRA--------EDNLVDWARPYLGDKRRLFRIMDTKLE 86
           G LT+KSDVYSFGVVLLE+L+G+R+        E NLV+W RP+L DK+R +R++D +LE
Sbjct: 311 GHLTTKSDVYSFGVVLLEILTGRRSVDKSRPNGEQNLVEWVRPHLLDKKRFYRLLDPRLE 370

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLS 146
           G YS KGA  A  +A+QCL  + +ARP+M+E++ AL+ L + K     S S Q   P   
Sbjct: 371 GHYSIKGAQKATQVAAQCLNRDSKARPKMSEVVEALKPLPNLKDFASSSSSFQTMQPVAK 430

Query: 147 NS-NVDGSILTSHQKSPQR 157
           N     G    S    P R
Sbjct: 431 NGVRTQGGGFVSRNGPPMR 449

[Close]

BLAST of Lsi08G007410 vs. ExPASy TrEMBL
Match: A0A5A7T4R2 (Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00870 PE=3 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 4.1e-57
Identity = 117/130 (90.00%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWA PYLGDKRRLFRIMDTKLEGRYS+KGA
Sbjct: 267 GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWASPYLGDKRRLFRIMDTKLEGRYSKKGA 326

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCLR+EPRARPRMAE+LGALEELE+PKTP RISHSVQ  TPTLSNSN DGS+
Sbjct: 327 YVAANLASQCLRNEPRARPRMAEVLGALEELETPKTPARISHSVQH-TPTLSNSNTDGSL 386

Query: 147 LTSHQKSPQR 157
            T HQKSPQ+
Sbjct: 387 STPHQKSPQQ 395

[Close]

BLAST of Lsi08G007410 vs. ExPASy TrEMBL
Match: A0A1S3C2W0 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496405 PE=3 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 4.1e-57
Identity = 117/130 (90.00%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWA PYLGDKRRLFRIMDTKLEGRYS+KGA
Sbjct: 267 GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWASPYLGDKRRLFRIMDTKLEGRYSKKGA 326

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCLR+EPRARPRMAE+LGALEELE+PKTP RISHSVQ  TPTLSNSN DGS+
Sbjct: 327 YVAANLASQCLRNEPRARPRMAEVLGALEELETPKTPARISHSVQH-TPTLSNSNTDGSL 386

Query: 147 LTSHQKSPQR 157
            T HQKSPQ+
Sbjct: 387 STPHQKSPQQ 395

[Close]

BLAST of Lsi08G007410 vs. ExPASy TrEMBL
Match: A0A0A0KE20 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G404180 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.5e-54
Identity = 114/129 (88.37%), Postives = 120/129 (93.02%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSGQRAEDNLV+ A PYLGDKR+LFRIMDTKLEGRYS+KGA
Sbjct: 267 GRLTSKSDVYSFGVVLLELLSGQRAEDNLVERASPYLGDKRKLFRIMDTKLEGRYSKKGA 326

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCL SEPRARPRMAEILGALEELE+PKTP RISHSVQR+TPTLSNSN DGS+
Sbjct: 327 YVAANLASQCLTSEPRARPRMAEILGALEELETPKTPARISHSVQRSTPTLSNSNPDGSL 386

Query: 147 LTSHQKSPQ 156
              HQKS Q
Sbjct: 387 SKPHQKSQQ 395

[Close]

BLAST of Lsi08G007410 vs. ExPASy TrEMBL
Match: A0A6J1KHV0 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita maxima OX=3661 GN=LOC111493974 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 6.2e-53
Identity = 112/130 (86.15%), Postives = 116/130 (89.23%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSG RAEDNLV+WA PYLGDKRRLFRIMDTKLEGRYS+KGA
Sbjct: 270 GRLTSKSDVYSFGVVLLELLSGLRAEDNLVEWAMPYLGDKRRLFRIMDTKLEGRYSKKGA 329

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCLR EPRARPRM EILG LEELESPKT  RISHSVQR T  LSNS VD S+
Sbjct: 330 YVAANLASQCLRREPRARPRMTEILGELEELESPKTAARISHSVQRETIALSNSKVDASL 389

Query: 147 LTSHQKSPQR 157
            T +QKSPQR
Sbjct: 390 STLYQKSPQR 399

[Close]

BLAST of Lsi08G007410 vs. ExPASy TrEMBL
Match: A0A6J1G338 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=LOC111450344 PE=3 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.8e-52
Identity = 110/130 (84.62%), Postives = 115/130 (88.46%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSG  AEDNLV+WA PYLGDKRRLFRIMDTKLEGRYS+ GA
Sbjct: 270 GRLTSKSDVYSFGVVLLELLSGLHAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKNGA 329

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCLR EPRARPRM EILG LEELESPKT  RISHSVQR TP LSNS VD S+
Sbjct: 330 YVAANLASQCLRREPRARPRMTEILGELEELESPKTTARISHSVQRETPALSNSKVDASL 389

Query: 147 LTSHQKSPQR 157
            T +QKSP+R
Sbjct: 390 STLYQKSPRR 399

[Close]

BLAST of Lsi08G007410 vs. NCBI nr
Match: XP_038884960.1 (probable serine/threonine-protein kinase PBL3 [Benincasa hispida])

HSP 1 Score: 236.1 bits (601), Expect = 2.0e-58
Identity = 119/130 (91.54%), Postives = 124/130 (95.38%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWA PYLGDKR+LFRIMDTKLEGRYS+KGA
Sbjct: 268 GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWATPYLGDKRKLFRIMDTKLEGRYSKKGA 327

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANL SQCLRSEPRARPRMAEILGALEE+ESPKT  RISH VQRTTPTLSNSNVDGS+
Sbjct: 328 YVAANLVSQCLRSEPRARPRMAEILGALEEIESPKTLARISHPVQRTTPTLSNSNVDGSL 387

Query: 147 LTSHQKSPQR 157
            TSHQKSP+R
Sbjct: 388 STSHQKSPRR 397

[Close]

BLAST of Lsi08G007410 vs. NCBI nr
Match: XP_008456461.1 (PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo] >KAA0036445.1 protein kinase 2B [Cucumis melo var. makuwa] >TYK00041.1 protein kinase 2B [Cucumis melo var. makuwa])

HSP 1 Score: 230.7 bits (587), Expect = 8.5e-57
Identity = 117/130 (90.00%), Postives = 123/130 (94.62%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWA PYLGDKRRLFRIMDTKLEGRYS+KGA
Sbjct: 267 GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWASPYLGDKRRLFRIMDTKLEGRYSKKGA 326

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCLR+EPRARPRMAE+LGALEELE+PKTP RISHSVQ  TPTLSNSN DGS+
Sbjct: 327 YVAANLASQCLRNEPRARPRMAEVLGALEELETPKTPARISHSVQH-TPTLSNSNTDGSL 386

Query: 147 LTSHQKSPQR 157
            T HQKSPQ+
Sbjct: 387 STPHQKSPQQ 395

[Close]

BLAST of Lsi08G007410 vs. NCBI nr
Match: XP_004146751.1 (probable serine/threonine-protein kinase PBL3 [Cucumis sativus])

HSP 1 Score: 222.2 bits (565), Expect = 3.0e-54
Identity = 114/129 (88.37%), Postives = 120/129 (93.02%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSGQRAEDNLV+ A PYLGDKR+LFRIMDTKLEGRYS+KGA
Sbjct: 267 GRLTSKSDVYSFGVVLLELLSGQRAEDNLVERASPYLGDKRKLFRIMDTKLEGRYSKKGA 326

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCL SEPRARPRMAEILGALEELE+PKTP RISHSVQR+TPTLSNSN DGS+
Sbjct: 327 YVAANLASQCLTSEPRARPRMAEILGALEELETPKTPARISHSVQRSTPTLSNSNPDGSL 386

Query: 147 LTSHQKSPQ 156
              HQKS Q
Sbjct: 387 SKPHQKSQQ 395

[Close]

BLAST of Lsi08G007410 vs. NCBI nr
Match: KAE8647295.1 (hypothetical protein Csa_003711 [Cucumis sativus])

HSP 1 Score: 222.2 bits (565), Expect = 3.0e-54
Identity = 114/129 (88.37%), Postives = 120/129 (93.02%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSGQRAEDNLV+ A PYLGDKR+LFRIMDTKLEGRYS+KGA
Sbjct: 44  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVERASPYLGDKRKLFRIMDTKLEGRYSKKGA 103

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCL SEPRARPRMAEILGALEELE+PKTP RISHSVQR+TPTLSNSN DGS+
Sbjct: 104 YVAANLASQCLTSEPRARPRMAEILGALEELETPKTPARISHSVQRSTPTLSNSNPDGSL 163

Query: 147 LTSHQKSPQ 156
              HQKS Q
Sbjct: 164 SKPHQKSQQ 172

[Close]

BLAST of Lsi08G007410 vs. NCBI nr
Match: XP_022999699.1 (probable serine/threonine-protein kinase PBL3 [Cucurbita maxima])

HSP 1 Score: 216.9 bits (551), Expect = 1.3e-52
Identity = 112/130 (86.15%), Postives = 116/130 (89.23%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWARPYLGDKRRLFRIMDTKLEGRYSQKGA 86
           GRLTSKSDVYSFGVVLLELLSG RAEDNLV+WA PYLGDKRRLFRIMDTKLEGRYS+KGA
Sbjct: 270 GRLTSKSDVYSFGVVLLELLSGLRAEDNLVEWAMPYLGDKRRLFRIMDTKLEGRYSKKGA 329

Query: 87  YVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLSNSNVDGSI 146
           YVAANLASQCLR EPRARPRM EILG LEELESPKT  RISHSVQR T  LSNS VD S+
Sbjct: 330 YVAANLASQCLRREPRARPRMTEILGELEELESPKTAARISHSVQRETIALSNSKVDASL 389

Query: 147 LTSHQKSPQR 157
            T +QKSPQR
Sbjct: 390 STLYQKSPQR 399

[Close]

BLAST of Lsi08G007410 vs. TAIR 10
Match: AT2G02800.1 (protein kinase 2B )

HSP 1 Score: 141.0 bits (354), Expect = 8.3e-34
Identity = 81/139 (58.27%), Postives = 100/139 (71.94%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRA--------EDNLVDWARPYLGDKRRLFRIMDTKLE 86
           GRLT+KSDVYSFGVVLLELLSG+RA        E +LVDWA PYLGDKR+LFRIMDT+L 
Sbjct: 268 GRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLG 327

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRI-SHSVQRTTPTL 146
           G+Y QKGAY AA+LA QCL  + + RP+M+E+L  L++LES K  T + +   Q  +P  
Sbjct: 328 GQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPRG 387

Query: 147 SNSNVDGSILTSHQKSPQR 157
           SN    GSI+   QKSP+R
Sbjct: 388 SN----GSIV---QKSPRR 399

[Close]

BLAST of Lsi08G007410 vs. TAIR 10
Match: AT2G02800.2 (protein kinase 2B )

HSP 1 Score: 141.0 bits (354), Expect = 8.3e-34
Identity = 81/139 (58.27%), Postives = 100/139 (71.94%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRA--------EDNLVDWARPYLGDKRRLFRIMDTKLE 86
           GRLT+KSDVYSFGVVLLELLSG+RA        E +LVDWA PYLGDKR+LFRIMDT+L 
Sbjct: 268 GRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLG 327

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRI-SHSVQRTTPTL 146
           G+Y QKGAY AA+LA QCL  + + RP+M+E+L  L++LES K  T + +   Q  +P  
Sbjct: 328 GQYPQKGAYTAASLALQCLNPDAKLRPKMSEVLAKLDQLESTKPGTGVGNRQAQIDSPRG 387

Query: 147 SNSNVDGSILTSHQKSPQR 157
           SN    GSI+   QKSP+R
Sbjct: 388 SN----GSIV---QKSPRR 399

[Close]

BLAST of Lsi08G007410 vs. TAIR 10
Match: AT1G14370.1 (protein kinase 2A )

HSP 1 Score: 138.7 bits (348), Expect = 4.1e-33
Identity = 85/158 (53.80%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 7   VSVKVITKFLYWFLDSFRRKGRLTSKSDVYSFGVVLLELLSGQRAEDN--------LVDW 66
           VS KVI    Y     +   GRLT+KSDVYSFGVVLLEL+SG+RA DN        LVDW
Sbjct: 252 VSTKVIGTHGY-AAPEYVATGRLTAKSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDW 311

Query: 67  ARPYLGDKRRLFRIMDTKLEGRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELE 126
           A PYLGDKR+LFRIMDTKL G+Y QKGA+ AANLA QCL  + + RP+M+E+L  LE+LE
Sbjct: 312 ATPYLGDKRKLFRIMDTKLGGQYPQKGAFTAANLALQCLNPDAKLRPKMSEVLVTLEQLE 371

Query: 127 SPKTPTRISHSVQRTTPTLSNSNVDGSILTSHQKSPQR 157
           S   P   +   Q  +P   +S+V        QKSP R
Sbjct: 372 SVAKPG--TKHTQMESPRFHHSSV-------MQKSPVR 399

[Close]

BLAST of Lsi08G007410 vs. TAIR 10
Match: AT1G26970.1 (Protein kinase superfamily protein )

HSP 1 Score: 132.9 bits (333), Expect = 2.3e-31
Identity = 70/121 (57.85%), Postives = 86/121 (71.07%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQRAED--------NLVDWARPYLGDKRRLFRIMDTKLE 86
           GR+T+KSDVYSFGVVLLELLSG+   D        NLVDWA PYLGDKR++FRIMDTKL 
Sbjct: 267 GRITAKSDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTKLG 326

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELESPKTPTRISHSVQRTTPTLS 140
           G+Y  KGA + AN A QCL  EP+ RP+M+++L  LEELE       IS+SV + T + S
Sbjct: 327 GQYPHKGACLTANTALQCLNQEPKLRPKMSDVLSTLEELEMTLKSGSISNSVMKLTSSSS 386

[Close]

BLAST of Lsi08G007410 vs. TAIR 10
Match: AT1G69790.1 (Protein kinase superfamily protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.0e-28
Identity = 63/101 (62.38%), Postives = 77/101 (76.24%), Query Frame = 0


Query: 27  GRLTSKSDVYSFGVVLLELLSGQ--------RAEDNLVDWARPYLGDKRRLFRIMDTKLE 86
           GRLTSKSDVYSFGVVLLELLSG+          E NLVDWA PYL D+R++FRIMDTKL 
Sbjct: 267 GRLTSKSDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTKLG 326

Query: 87  GRYSQKGAYVAANLASQCLRSEPRARPRMAEILGALEELES 120
           G+Y  KGA  AAN+A +CL +EP+ RP MA++L  L++LE+
Sbjct: 327 GQYPHKGACAAANIALRCLNTEPKLRPDMADVLSTLQQLET 367

The following BLAST results are available for this feature:

BLAST of Lsi08G007410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49840	1.2e-32	58.27	Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49839	5.8e-32	53.80	Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q5PP29	3.2e-30	57.85	Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q5XF79	1.5e-27	62.38	Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G...	[more]
F4J0D2	2.0e-24	45.32	Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 P...	[more]

BLAST of Lsi08G007410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T4R2	4.1e-57	90.00	Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00...	[more]
A0A1S3C2W0	4.1e-57	90.00	protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496405 PE...	[more]
A0A0A0KE20	1.5e-54	88.37	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G404...	[more]
A0A6J1KHV0	6.2e-53	86.15	probable serine/threonine-protein kinase PBL3 OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1G338	1.8e-52	84.62	probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of Lsi08G007410 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884960.1	2.0e-58	91.54	probable serine/threonine-protein kinase PBL3 [Benincasa hispida]	[more]
XP_008456461.1	8.5e-57	90.00	PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis melo] >KAA0036445.1 pr...	[more]
XP_004146751.1	3.0e-54	88.37	probable serine/threonine-protein kinase PBL3 [Cucumis sativus]	[more]
KAE8647295.1	3.0e-54	88.37	hypothetical protein Csa_003711 [Cucumis sativus]	[more]
XP_022999699.1	1.3e-52	86.15	probable serine/threonine-protein kinase PBL3 [Cucurbita maxima]	[more]

BLAST of Lsi08G007410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02800.1	8.3e-34	58.27	protein kinase 2B	[more]
AT2G02800.2	8.3e-34	58.27	protein kinase 2B	[more]
AT1G14370.1	4.1e-33	53.80	protein kinase 2A	[more]
AT1G26970.1	2.3e-31	57.85	Protein kinase superfamily protein	[more]
AT1G69790.1	1.0e-28	62.38	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	PFAM	PF07714	PK_Tyr_Ser-Thr	coord: 25..112 e-value: 2.0E-7 score: 30.6
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 10..138 e-value: 4.8E-21 score: 76.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 121..156
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 123..156
None	No IPR available	PANTHER	PTHR45621	OS01G0588500 PROTEIN-RELATED	coord: 7..128
None	No IPR available	PANTHER	PTHR45621:SF109	PROTEIN KINASE 2B, CHLOROPLASTIC	coord: 7..128
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 1..117 score: 10.195574
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 25..141

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G007410.1	Lsi08G007410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004674	protein serine/threonine kinase activity
molecular_function	GO:0004672	protein kinase activity