Lsi08G007230 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G007230
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionmRNA-decapping enzyme subunit 2-like
Locationchr08: 15593548 .. 15598214 (+)
RNA-Seq ExpressionLsi08G007230
SyntenyLsi08G007230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGGGTCCGGTCGAACCGGTCGAGCAAATAACATGAACCGGGCCGTGGATATTTGATAGAATTGAGTCAATTGACAATATCGGGTTTTATCGTTTCGCGGCTACTTGGTTTGTGTTTGGTGAAAAGCTTTTTCATCGAAGGGTCGACGAAAAGGAGCTGAAAATCGTCATCTCGTTTCTCCTCCATTTCCAATTCCGAACAACTCCATGGCCGCTCTTCACCACTCCTCTTCCACTGCTCCGGTCAAAAACGGCCTTCCTCCTCAAGAACTCCTCGACGATCTTTGCAGGTCTGTTTTTCAACTCAACTCAACTCTCTCTCTCTCTTTCCACTTTTGTTCTTTCTTCTTGTTCTTGAACTCCTCCATTATGTGCTTCCAGCATCACATTCTTTTCATATTTGCTTCATATGACTGATCCTTCCACTCAGTTCTTTTTTCCTTTTTGTTCTAATGTCTTTTTCACATTCTATTGATTTCTGTTCTGCAACGCGACTGAGAACTTTTGTTCGATCGTAATTGCGGTAAATAGTGAAAGAAGAGTAGTTGGTTTACTTGCTTGAAGTTCTCCTTAAAAACGTCGATGAAGTGTAGGGAGTTGAAATTTGGAATAGAAATAAGGTTTATGAGTAAGGAACATAGTGGATCGGCTACACGGATGTTCCTTGTATGTATGTGCATTTTGTTTCTGTTTGAGTGATTTCTCTACTTACTTAGCTTAAACATGCTTAATGATGAACAATTTCTGTTTATGATAGCGAGTATTGTTTGTTCATATCTTTGTTAAGAGGAGTTCGTTGGCACATTACTGAATGGGCGGTTGGATTTTTTTTTTTCCTTTCTCGAAGCAACGTTATGTTCTTTAATGTAGTAAAAGGCTAAGAAATTGTGGCGTTGAATACTGATGTGATGAGTGCTTATTTGATATTGTGGTGGATCTTTTAGAATAAAGTTTCCAGAACTGATTTAGTAAGAAATTTCTCACATAGTTATGAACAGATAGATGGGAATTTATATTAGATAACTTAGTTGTTCCTTGAACAAAAGTTGTTGTTCCTTAGACTTAAATCCACTTCATCCTTAGTCTTTGTATGAGATTTGACCTTGATGGATCTTCAATTCATTCTTCGTTAGTTTCCTTATTTTCTAAGCTGATAGTCTCTAGATGCAGCTCTGAATCTCTTTTCCTCTTCCTTTATTTACTTCAATATTTTGTGTTGATTTTCTGCAGTCGGTTTGTTTTGAATGTGCCGAAAGAAGATCTGCAGTCATTTGAGAGGATTCTATTCCTCATCGAGTATGCACATTGGTTTTATGAAGACAATTCGGTGGAAAGGAATCCATCTTTAAAGTCATTGAATCTGAAGGAGTTCACTTCATTATGTATCCTGCACAGTTGTGCAACCATTTGATTATGGAAAAAGAATAGTCGGAGTCCTCTCTCTCTATATCTCTCTCAAATGATGATTAATACTTCTTTTAGTGGTTTACTTTATTACTCTCTTATTAATCCAAATTAATGTGCCTATTATCTGCGATTCTGCACATTCCTGTTTTCCCTTATTATATATCAGTGTTTAAAAGCTGCGATGTCTTAAGACCATATGTTGCCCATATTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATAATGGATGAGACATTTGAGCGGGTGAGCTCTTGTTAATGAAACTTGGCTCACTTAAAATTGTTTAAGCTGTTCTTTCCCCCACATAATTGATGTGAAGGATGTTGACACTGCTCCCGTCTGCTTCTTATTGGCAAAATACAGTGCTTGCTAGTGAAGGGATGGAAAGGATCAAGTTGGAGTTTTCCACGCGGTAAAAAGAGCAAAGATGAGGAAGACCATGCTTGCGCCATTAGAGAAGTAATCTTGGTTCTTTTCGAAATTTCTCATTGCATATTCAGCATTCTCTTGTAAATACTACTTGGATTGTAACTGCAAAATTCACTTGAAGTTTAATGAATTAGAACTTTACAAGTTATCAGATGTTATTCCATTTCCTTCTGTGGGATCTGGGCAGGAAAGATTGAATTTTAGTTCAATAATGTCCTGTATAGATGGAGAAAAGTTTCTCTGGTATCGGGAAAATTCTCAAACATTGATTTCCGGCTGACAGACTATGTGTCTCCTATAAATGTGAGATTTACTATTTGATTGCAGGTCCTGGAAGAAACAGGATTTGACATTACAAATCTTCTCAACAAATCTGAATACATAGAAGTTATATTTGGTCAGCAAAGGGTGCGGCTTTACATCATTGCTGGTGTGAAAGATGATAATCTCTTTGCCCCGCAAACTAAGAAAGAGATCAGTGTATGTGTTTTCTTCTTACCTCAAAATATATTTCTAGAGTTTTAGTTTTGATTTGCATTGAAATCACCACCGCAGAATATGTTTCTTAAAGTTTTAGTTTTTTCATGCATGTGTTGCCTTTCTTCAGGTCTTTTGACAACCATTCTTATGATATTTTAAGCATTTATAATCCTCGTTTAACTCCTAAAAATTGTCTTGTAGCCAATCTTGAAGGATCGTAATTTTAAATTTTAAATCTTCTCCTGTATTTGTGACATTTTATTCTTAAAAGAAAAAGTCTCCTTCTTGTAAAGGTTTTGGTAATTATGAACTATCATCTGATTCTGGATTATGCTAGTGTGACCATGATGTAATGGAAGAAAGATGCATCGATGAATCTCGAGTTTAATCTTTTCTTTTATGCAGTATTGTAGAATATAAATGCGTTGTAGACTTAATCCATGCAAGATTTTTTTTTTTTTGAGATCTAATAGTTTTAGGTTGCAAGCTATAAAATTTTTCTAGAAAACTCATGTTATCTAATATGATATTAGATTCAAGTTTAAATAGGTATTTGGTCCAAAAAAAAAAATTTAATTCAAGAATGGTAAATCTAGAGAGGCATCATCGTGAGGGACATGTTAAAAAGATAAATGAGTTATTTCTCTCGTGGTCAATAAATTTTGAGATGAAACTTCATATTTGTATAATATTCCAGCTTTAAATTTACCTTGTCAGGTTTTAAAAAAAATTCAAAGGGCTGTAATACTTGAAGGGCTGAGAATGAAAATGTTTTTGGATTCAACTTAGAGGTTTGGTTCCAGAATTCTTGGGGATGGATGTCTTAAAAATTAAACATGTCCCATGCCTATCAAATGTTGAAAAGTTAGGCGTGTATTTTTTAATTTTGAGCCCAGAGAAGATGGGTTTGACAAATTTCTGGGTAAAGGGAAAAGGGGGAATTGTTTATTAGTGGTAGGTCATCATTGGAATCTTCTGTAGTTGAAAGCACGTACTTAACTATTTGTAAACAGGAAATTGCATGGCATCGACTCGATGATCTTGAACCAGCTAGTGATAATGTAATATCTCGTGGGATGAACGGTCTCAAGCTTTATATGGTTGCTCCTTTTTTGGCGTGAGTACATCTGTATATTCTTAATGATTTCTTCCTCATGCTTTAACGAATGTAGATGTTTTAAGGGGTCAGCATCTTCATGCAGATCTCTGAGGTCATGGATTTCGACACATCATCCCCCTGTAGCTCCAAACCCTGACGTGTCAATAAGAGGTTTTCTTTTCTCCACTTATTAGGGATTATGAAATTTTGCAGTTATTATCAATACGAGTTTGCTTCTGATTTCCAACAATAGATTCTGTTTTTCAAATCAAGTTATAGACCGTCCAAATTCCAAGGCATAATCTCACAACTTTACATTCATTAACTTACATAAACTTCCTTCCTAGTTTTTATTAGAAGCCTTTGTGAAATATGTTAATGTAGTGTCTGTTCTTGTTTTCAAGGACTAACGGTATGGAAGGCTAAGAACAGCTCGATAGGTAGCAGCAGTACAATCATAATGGAGAGCCAATTAAGTAAACAAGAAGTGGATGTTCCCATCCCAGACACTGGTCCAGGCAGGAGCTTCAGAAACTTCAAGTTTGATACTGCCTCAATCTTACAGTCAATGGAAAGAGCCTTTTCTTAAGTCTTCTACAACTCCTAAAGACCTTTTTTTCTTAGTTGGCTCATGCTTAGACCTTATAGATATGTCAAATTACTGACTAATATAGTACTAGCAAAAGAAAAAAAAAAAGAAGAAGAAGAAAAAAAATAGGAGTGATAGCACAGACAGAGGGAGGGAAAGTGATTTGATCTACCCAACCATCCTCCTCCATGCTATTTTGGACATAAAGTGTTGGTTTAGAGTTCTTATTTTGTGTGAAATTTGCGTATATTCTGTTAGTCTATACAAAAGTGTCGGTGTGCAAATGTCTAGAAAAAGGAAAAAAAGGGCTCCAACAATTGCATCAATTTGGTGTTCTTGTGGTGAAAGTGAGATGGAGAAATCACTTCTTGATGTACTTTGCCAGCTTGATTGCAATGACAGGTGTCGGTGCCAGCTTGATTGCAACGACAAGTGTCGGTTCGTTGGAGTTCATGTCTATGCCACATGGGATTTTCTGTACAGAAACATTGATGTGGAAAATGTGTTGAACTTTGATGTATGTTTACTTTAGTTTTTATCAATTTGGTTTGGTAGTGTTGTGGGGAGAGCACTTTTAGAAGACATGCTGCTGCTCTTACCCCTTTTTCC

mRNA sequence

AAAAAAGGGTCCGGTCGAACCGGTCGAGCAAATAACATGAACCGGGCCGTGGATATTTGATAGAATTGAGTCAATTGACAATATCGGGTTTTATCGTTTCGCGGCTACTTGGTTTGTGTTTGGTGAAAAGCTTTTTCATCGAAGGGTCGACGAAAAGGAGCTGAAAATCGTCATCTCGTTTCTCCTCCATTTCCAATTCCGAACAACTCCATGGCCGCTCTTCACCACTCCTCTTCCACTGCTCCGGTCAAAAACGGCCTTCCTCCTCAAGAACTCCTCGACGATCTTTGCAGTCGGTTTGTTTTGAATGTGCCGAAAGAAGATCTGCAGTCATTTGAGAGGATTCTATTCCTCATCGAGTATGCACATTGGTTTTATGAAGACAATTCGGTGGAAAGGAATCCATCTTTAAAGTCATTGAATCTGAAGGAGTTCACTTCATTATTGTTTAAAAGCTGCGATGTCTTAAGACCATATGTTGCCCATATTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATAATGGATGAGACATTTGAGCGGTGCTTGCTAGTGAAGGGATGGAAAGGATCAAGTTGGAGTTTTCCACGCGGTAAAAAGAGCAAAGATGAGGAAGACCATGCTTGCGCCATTAGAGAAGTCCTGGAAGAAACAGGATTTGACATTACAAATCTTCTCAACAAATCTGAATACATAGAAGTTATATTTGGTCAGCAAAGGGTGCGGCTTTACATCATTGCTGGTGTGAAAGATGATAATCTCTTTGCCCCGCAAACTAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTCGATGATCTTGAACCAGCTAGTGATAATGTAATATCTCGTGGGATGAACGGTCTCAAGCTTTATATGGTTGCTCCTTTTTTGGCATCTCTGAGGTCATGGATTTCGACACATCATCCCCCTGTAGCTCCAAACCCTGACGTGTCAATAAGAGTTATTATCAATACGAGTTTGCTTCTGATTTCCAACAATAGATTCTGTTTTTCAAATCAAACCGTCCAAATTCCAAGGCATAATCTCACAACTTTACATTCATTAACTTACATAAACTTCCTTCCTAGACTAACGGTATGGAAGGCTAAGAACAGCTCGATAGGTAGCAGCAGTACAATCATAATGGAGAGCCAATTAAGTAAACAAGAAGTGGATGTTCCCATCCCAGACACTGGTCCAGGCAGGAGCTTCAGAAACTTCAAGTTTGATACTGCCTCAATCTTACATCTATACAAAAGTGTCGGTGTGCAAATGTCTAGAAAAAGGAAAAAAAGGGCTCCAACAATTGCATCAATTTGGTGTTCTTGTGGTGAAAGTGAGATGGAGAAATCACTTCTTGATGTACTTTGCCAGCTTGATTGCAATGACAGGTGTCGGTGCCAGCTTGATTGCAACGACAAGTGTCGGTTCGTTGGAGTTCATGTCTATGCCACATGGGATTTTCTGTACAGAAACATTGATGTGGAAAATGTGTTGAACTTTGATTGTTGTGGGGAGAGCACTTTTAGAAGACATGCTGCTGCTCTTACCCCTTTTTCC

Coding sequence (CDS)

ATGGCCGCTCTTCACCACTCCTCTTCCACTGCTCCGGTCAAAAACGGCCTTCCTCCTCAAGAACTCCTCGACGATCTTTGCAGTCGGTTTGTTTTGAATGTGCCGAAAGAAGATCTGCAGTCATTTGAGAGGATTCTATTCCTCATCGAGTATGCACATTGGTTTTATGAAGACAATTCGGTGGAAAGGAATCCATCTTTAAAGTCATTGAATCTGAAGGAGTTCACTTCATTATTGTTTAAAAGCTGCGATGTCTTAAGACCATATGTTGCCCATATTGATGATATCTTTAAAGACTTCACTTCATACAAGGTTCGAGTTCCTGTAACTGGGGCAATCATAATGGATGAGACATTTGAGCGGTGCTTGCTAGTGAAGGGATGGAAAGGATCAAGTTGGAGTTTTCCACGCGGTAAAAAGAGCAAAGATGAGGAAGACCATGCTTGCGCCATTAGAGAAGTCCTGGAAGAAACAGGATTTGACATTACAAATCTTCTCAACAAATCTGAATACATAGAAGTTATATTTGGTCAGCAAAGGGTGCGGCTTTACATCATTGCTGGTGTGAAAGATGATAATCTCTTTGCCCCGCAAACTAAGAAAGAGATCAGTGAAATTGCATGGCATCGACTCGATGATCTTGAACCAGCTAGTGATAATGTAATATCTCGTGGGATGAACGGTCTCAAGCTTTATATGGTTGCTCCTTTTTTGGCATCTCTGAGGTCATGGATTTCGACACATCATCCCCCTGTAGCTCCAAACCCTGACGTGTCAATAAGAGTTATTATCAATACGAGTTTGCTTCTGATTTCCAACAATAGATTCTGTTTTTCAAATCAAACCGTCCAAATTCCAAGGCATAATCTCACAACTTTACATTCATTAACTTACATAAACTTCCTTCCTAGACTAACGGTATGGAAGGCTAAGAACAGCTCGATAGGTAGCAGCAGTACAATCATAATGGAGAGCCAATTAAGTAAACAAGAAGTGGATGTTCCCATCCCAGACACTGGTCCAGGCAGGAGCTTCAGAAACTTCAAGTTTGATACTGCCTCAATCTTACATCTATACAAAAGTGTCGGTGTGCAAATGTCTAGAAAAAGGAAAAAAAGGGCTCCAACAATTGCATCAATTTGGTGTTCTTGTGGTGAAAGTGAGATGGAGAAATCACTTCTTGATGTACTTTGCCAGCTTGATTGCAATGACAGGTGTCGGTGCCAGCTTGATTGCAACGACAAGTGTCGGTTCGTTGGAGTTCATGTCTATGCCACATGGGATTTTCTGTACAGAAACATTGATGTGGAAAATGTGTTGAACTTTGATTGTTGTGGGGAGAGCACTTTTAGAAGACATGCTGCTGCTCTTACCCCTTTTTCC

Protein sequence

MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQRVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLASLRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYINFLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSFRNFKFDTASILHLYKSVGVQMSRKRKKRAPTIASIWCSCGESEMEKSLLDVLCQLDCNDRCRCQLDCNDKCRFVGVHVYATWDFLYRNIDVENVLNFDCCGESTFRRHAAALTPFS
Homology
BLAST of Lsi08G007230 vs. ExPASy Swiss-Prot
Match: Q8GW31 (mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 2.2e-119
Identity = 229/376 (60.90%), Postives = 273/376 (72.61%), Query Frame = 0

Query: 1   MAALHHSSSTAP-VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDN 60
           M+ LH SSS++  + N LP +ELLDDLCSRFVLNVP+ED QSFERILFL+EYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETF 120
           +VE +P LKSL+LKEFTSLLF SCDVLRPYV HIDDIFKDFTSYK RVPVTGAII+DET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFD++ LL + EYIE +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLA 240
           RVRLYI+AGV +D +FAP TKKEISEI WHRLD L+PAS+ VI+ G++GLKLYMVAPFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCF-SNQTVQIPRHN------LTT 300
           SL+SWI  H  PVA  P+  ++       L + N R     N T  +   N       TT
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKA------LCVWNARTSVGGNGTATVESQNRKSELRTTT 300

Query: 301 LHSLTYINFLPRLTVWK-------AKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSF 360
           + S +    L R T+          K +    ++T  +ES  +K  VD    D  PG SF
Sbjct: 301 MESNSRKPELKRTTMESHSTKPELRKGTMESHNTTATVESHNTKPVVD-HSQDIKPGGSF 360

Query: 361 RNFKFDTASILHLYKS 362
            NFKF+ + IL   +S
Sbjct: 361 INFKFNQSVILQALES 369

BLAST of Lsi08G007230 vs. ExPASy Swiss-Prot
Match: O13828 (mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dcp2 PE=1 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 4.0e-57
Identity = 119/257 (46.30%), Postives = 161/257 (62.65%), Query Frame = 0

Query: 21  ELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSLLF 80
           ++LDDL +RF+LN+P E+  S ER+ F IE AHWFYED    +N  L SL L+ F++ LF
Sbjct: 10  QVLDDLSARFILNLPAEEQSSVERLCFQIEQAHWFYEDFIRAQNDQLPSLGLRVFSAKLF 69

Query: 81  KSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKGSS-WSFPRGK 140
             C +L  +    ++ F DF  YK R+PV GAI++D + ++C+LVKGWK SS W FP+GK
Sbjct: 70  AHCPLLWKWSKVHEEAFDDFLRYKTRIPVRGAIMLDMSMQQCVLVKGWKASSGWGFPKGK 129

Query: 141 KSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQRVRLYIIAGVKDDNLFAPQT 200
             KDE D  CAIREV EETGFD ++ +N +E+I++    Q VRLYII G+  D  F  +T
Sbjct: 130 IDKDESDVDCAIREVYEETGFDCSSRINPNEFIDMTIRGQNVRLYIIPGISLDTRFESRT 189

Query: 201 KKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLASLRSWISTHHPPVAPN---- 260
           +KEIS+I WH L DL     N      N  K YMV PFLA L+ WI   +  +A N    
Sbjct: 190 RKEISKIEWHNLMDLPTFKKNKPQTMKN--KFYMVIPFLAPLKKWIKKRN--IANNTTKE 249

Query: 261 PDVSIRVIINTSLLLIS 273
            ++S+ V  + S  L+S
Sbjct: 250 KNISVDVDADASSQLLS 262

BLAST of Lsi08G007230 vs. ExPASy Swiss-Prot
Match: Q9CYC6 (m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 1.3e-50
Identity = 98/232 (42.24%), Postives = 138/232 (59.48%), Query Frame = 0

Query: 19  PQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSL 78
           P  +LDDLCSRF+L++P E+  +  R+ F IE AHWFY D  ++  P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 79  LFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKG-SSWSFPR 138
           +F  C  L P    ++ I  ++  YK+ VP  GAII+DET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKILDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 139 GKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQRVRLYIIAGVKDDNLFAP 198
           GK +K+E  H CA REV EETGFDI + + K +YIE+    Q  RLYII GV  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGVPKDTKFNP 188

Query: 199 QTKKEISEIAWHRLDDLEPASDNVISRGMNGL---KLYMVAPFLASLRSWIS 247
           +T++EI  I W  ++ L    +++  +   GL   K +M  PF+  LR W+S
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLS 240

BLAST of Lsi08G007230 vs. ExPASy Swiss-Prot
Match: Q8IU60 (m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3)

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-50
Identity = 96/232 (41.38%), Postives = 138/232 (59.48%), Query Frame = 0

Query: 19  PQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSL 78
           P  +LDDLCSRF+L++P E+  +  R+ F IE AHWFY D  ++  P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKA 68

Query: 79  LFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKG-SSWSFPR 138
           +F  C  L P    ++ +  ++  YK+ VP  GAII+DET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 139 GKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQRVRLYIIAGVKDDNLFAP 198
           GK +K+E  H CA REV EETGFDI + + K +YIE+    Q  RLYII G+  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 199 QTKKEISEIAWHRLDDLEPASDNVISRGMNGL---KLYMVAPFLASLRSWIS 247
           +T++EI  I W  ++ L    +++  +   GL   K +M  PF+  LR W+S
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLS 240

BLAST of Lsi08G007230 vs. ExPASy Swiss-Prot
Match: Q5REQ8 (m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-50
Identity = 96/232 (41.38%), Postives = 138/232 (59.48%), Query Frame = 0

Query: 19  PQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNSVERNPSLKSLNLKEFTSL 78
           P  +LDDLCSRF+L++P E+  +  R+ F IE AHWFY D  ++  P L    +++F   
Sbjct: 9   PGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLSQCGIRDFAKA 68

Query: 79  LFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFERCLLVKGWKG-SSWSFPR 138
           +F  C  L P    ++ +  ++  YK+ VP  GAII+DET E  LLV+G+   S W FP+
Sbjct: 69  VFSHCPFLLPEGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPK 128

Query: 139 GKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQRVRLYIIAGVKDDNLFAP 198
           GK +K+E  H CA REV EETGFDI + + K +YIE+    Q  RLYII G+  D  F P
Sbjct: 129 GKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNP 188

Query: 199 QTKKEISEIAWHRLDDLEPASDNVISRGMNGL---KLYMVAPFLASLRSWIS 247
           +T++EI  I W  ++ L    +++  +   GL   K +M  PF+  LR W+S
Sbjct: 189 KTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLS 240

BLAST of Lsi08G007230 vs. ExPASy TrEMBL
Match: A0A0A0KHG2 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G405350 PE=3 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 4.8e-162
Identity = 293/356 (82.30%), Postives = 303/356 (85.11%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAALHHSSST PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSL+FKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAII+DETFE
Sbjct: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NLL KSEYIEVIFG QR
Sbjct: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGM+GLKLYM+APFLAS
Sbjct: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWI THHPPV PNPD+ IR                                       
Sbjct: 292 LRSWILTHHPPVRPNPDIPIR--------------------------------------- 351

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSIGSSSTIIMESQ+SK EV+V   DTGPG+SFRNFKFDTASIL
Sbjct: 352 ---GLTVWKAKNSSIGSSSTIIMESQISKAEVEV---DTGPGKSFRNFKFDTASIL 362

BLAST of Lsi08G007230 vs. ExPASy TrEMBL
Match: A0A5D3BM04 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00650 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 8.2e-162
Identity = 294/356 (82.58%), Postives = 302/356 (84.83%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAAL HSSST PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAI++DETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NLLNKSEYIEVIFGQQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWI THHPPV  NPD+ IR                                       
Sbjct: 241 LRSWILTHHPPVHSNPDIPIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSI SSSTIIMESQ+SK EVDV   DTGPG+SFRNFKFDTASIL
Sbjct: 301 ---GLTVWKAKNSSISSSSTIIMESQISKAEVDV---DTGPGKSFRNFKFDTASIL 311

BLAST of Lsi08G007230 vs. ExPASy TrEMBL
Match: A0A1S3C3F4 (mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103496421 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 8.2e-162
Identity = 294/356 (82.58%), Postives = 302/356 (84.83%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAAL HSSST PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAI++DETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NLLNKSEYIEVIFGQQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWI THHPPV  NPD+ IR                                       
Sbjct: 241 LRSWILTHHPPVHSNPDIPIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSI SSSTIIMESQ+SK EVDV   DTGPG+SFRNFKFDTASIL
Sbjct: 301 ---GLTVWKAKNSSISSSSTIIMESQISKAEVDV---DTGPGKSFRNFKFDTASIL 311

BLAST of Lsi08G007230 vs. ExPASy TrEMBL
Match: A0A6J1JIL9 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487274 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 2.0e-160
Identity = 292/358 (81.56%), Postives = 303/358 (84.64%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAALH SSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAII+DET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNL+NKSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWISTH PPVA  PDVSIR                                       
Sbjct: 241 LRSWISTHQPPVASKPDVSIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPI--PDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSIGSSSTI++ESQLSK ++DV +  PDTGPG+SFRNFKFDTASI+
Sbjct: 301 ---GLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASII 316

BLAST of Lsi08G007230 vs. ExPASy TrEMBL
Match: A0A6J1HJG1 (mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111464573 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 7.7e-160
Identity = 291/358 (81.28%), Postives = 302/358 (84.36%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAALH SS TAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSCTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAII+DET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNL+NKSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWISTH PPVA  PDVSIR                                       
Sbjct: 241 LRSWISTHQPPVASKPDVSIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPI--PDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSIGSSSTI++ESQLSK ++DV +  PDTGPG+SFRNFKFDTASI+
Sbjct: 301 ---GLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASII 316

BLAST of Lsi08G007230 vs. NCBI nr
Match: XP_038883995.1 (mRNA-decapping enzyme subunit 2-like [Benincasa hispida])

HSP 1 Score: 600.9 bits (1548), Expect = 9.3e-168
Identity = 306/356 (85.96%), Postives = 308/356 (86.52%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAALHHSSSTAPVKN LP QELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHHSSSTAPVKNVLPSQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVK DN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKVDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWISTHHPPV PNPDVSIR                                       
Sbjct: 241 LRSWISTHHPPVHPNPDVSIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSIGSSSTIIMESQLSKQEVDV IPDTGPGRSFRNFKFDTASIL
Sbjct: 301 ---GLTVWKAKNSSIGSSSTIIMESQLSKQEVDVSIPDTGPGRSFRNFKFDTASIL 314

BLAST of Lsi08G007230 vs. NCBI nr
Match: XP_004149909.2 (mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN47822.1 hypothetical protein Csa_003258 [Cucumis sativus])

HSP 1 Score: 580.9 bits (1496), Expect = 9.9e-162
Identity = 293/356 (82.30%), Postives = 303/356 (85.11%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAALHHSSST PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 52  MAALHHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 111

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSL+FKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAII+DETFE
Sbjct: 112 VERNPSLKSLNLKEFTSLMFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIILDETFE 171

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NLL KSEYIEVIFG QR
Sbjct: 172 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLKKSEYIEVIFGPQR 231

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGM+GLKLYM+APFLAS
Sbjct: 232 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMSGLKLYMIAPFLAS 291

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWI THHPPV PNPD+ IR                                       
Sbjct: 292 LRSWILTHHPPVRPNPDIPIR--------------------------------------- 351

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSIGSSSTIIMESQ+SK EV+V   DTGPG+SFRNFKFDTASIL
Sbjct: 352 ---GLTVWKAKNSSIGSSSTIIMESQISKAEVEV---DTGPGKSFRNFKFDTASIL 362

BLAST of Lsi08G007230 vs. NCBI nr
Match: XP_008456478.1 (PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0036467.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa] >TYK00020.1 mRNA-decapping enzyme subunit 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 580.1 bits (1494), Expect = 1.7e-161
Identity = 294/356 (82.58%), Postives = 302/356 (84.83%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAAL HSSST PVKNGLPPQEL+DDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALQHSSSTTPVKNGLPPQELIDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAI++DETFE
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIVLDETFE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDI+NLLNKSEYIEVIFGQQR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDISNLLNKSEYIEVIFGQQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWI THHPPV  NPD+ IR                                       
Sbjct: 241 LRSWILTHHPPVHSNPDIPIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSI SSSTIIMESQ+SK EVDV   DTGPG+SFRNFKFDTASIL
Sbjct: 301 ---GLTVWKAKNSSISSSSTIIMESQISKAEVDV---DTGPGKSFRNFKFDTASIL 311

BLAST of Lsi08G007230 vs. NCBI nr
Match: XP_022990407.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita maxima])

HSP 1 Score: 575.5 bits (1482), Expect = 4.2e-160
Identity = 292/358 (81.56%), Postives = 303/358 (84.64%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAALH SSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAII+DET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNL+NKSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWISTH PPVA  PDVSIR                                       
Sbjct: 241 LRSWISTHQPPVASKPDVSIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPI--PDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSIGSSSTI++ESQLSK ++DV +  PDTGPG+SFRNFKFDTASI+
Sbjct: 301 ---GLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASII 316

BLAST of Lsi08G007230 vs. NCBI nr
Match: XP_022964616.1 (mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >XP_023540752.1 mRNA-decapping enzyme subunit 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 573.5 bits (1477), Expect = 1.6e-159
Identity = 291/358 (81.28%), Postives = 302/358 (84.36%), Query Frame = 0

Query: 1   MAALHHSSSTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60
           MAALH SS TAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS
Sbjct: 1   MAALHSSSCTAPVKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDNS 60

Query: 61  VERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETFE 120
           VERNPSLKSLNLKEFTSLLFKSCDVL+PYVAHIDDIFKDFTSYKVRVPVTGAII+DET E
Sbjct: 61  VERNPSLKSLNLKEFTSLLFKSCDVLKPYVAHIDDIFKDFTSYKVRVPVTGAIILDETSE 120

Query: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQR 180
           RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNL+NKSEYIEVIFG QR
Sbjct: 121 RCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLINKSEYIEVIFGPQR 180

Query: 181 VRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLAS 240
           VRLYIIAGVKDDN+FAPQTKKEISEIAWHRL DLEPA DNVISRGMNGLKLYMVAPFLAS
Sbjct: 181 VRLYIIAGVKDDNIFAPQTKKEISEIAWHRLGDLEPAGDNVISRGMNGLKLYMVAPFLAS 240

Query: 241 LRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCFSNQTVQIPRHNLTTLHSLTYIN 300
           LRSWISTH PPVA  PDVSIR                                       
Sbjct: 241 LRSWISTHQPPVASKPDVSIR--------------------------------------- 300

Query: 301 FLPRLTVWKAKNSSIGSSSTIIMESQLSKQEVDVPI--PDTGPGRSFRNFKFDTASIL 357
               LTVWKAKNSSIGSSSTI++ESQLSK ++DV +  PDTGPG+SFRNFKFDTASI+
Sbjct: 301 ---GLTVWKAKNSSIGSSSTIVIESQLSKPDMDVHVSTPDTGPGKSFRNFKFDTASII 316

BLAST of Lsi08G007230 vs. TAIR 10
Match: AT5G13570.1 (decapping 2 )

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-120
Identity = 229/376 (60.90%), Postives = 273/376 (72.61%), Query Frame = 0

Query: 1   MAALHHSSSTAP-VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDN 60
           M+ LH SSS++  + N LP +ELLDDLCSRFVLNVP+ED QSFERILFL+EYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETF 120
           +VE +P LKSL+LKEFTSLLF SCDVLRPYV HIDDIFKDFTSYK RVPVTGAII+DET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDITNLLNKSEYIEVIFGQQ 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFD++ LL + EYIE +F QQ
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIREVLEETGFDVSKLLKREEYIEFVFRQQ 180

Query: 181 RVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGMNGLKLYMVAPFLA 240
           RVRLYI+AGV +D +FAP TKKEISEI WHRLD L+PAS+ VI+ G++GLKLYMVAPFL+
Sbjct: 181 RVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGVSGLKLYMVAPFLS 240

Query: 241 SLRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCF-SNQTVQIPRHN------LTT 300
           SL+SWI  H  PVA  P+  ++       L + N R     N T  +   N       TT
Sbjct: 241 SLKSWILKHPSPVARRPNKPLKA------LCVWNARTSVGGNGTATVESQNRKSELRTTT 300

Query: 301 LHSLTYINFLPRLTVWK-------AKNSSIGSSSTIIMESQLSKQEVDVPIPDTGPGRSF 360
           + S +    L R T+          K +    ++T  +ES  +K  VD    D  PG SF
Sbjct: 301 MESNSRKPELKRTTMESHSTKPELRKGTMESHNTTATVESHNTKPVVD-HSQDIKPGGSF 360

Query: 361 RNFKFDTASILHLYKS 362
            NFKF+ + IL   +S
Sbjct: 361 INFKFNQSVILQALES 369

BLAST of Lsi08G007230 vs. TAIR 10
Match: AT5G13570.2 (decapping 2 )

HSP 1 Score: 421.4 bits (1082), Expect = 9.4e-118
Identity = 229/389 (58.87%), Postives = 273/389 (70.18%), Query Frame = 0

Query: 1   MAALHHSSSTAP-VKNGLPPQELLDDLCSRFVLNVPKEDLQSFERILFLIEYAHWFYEDN 60
           M+ LH SSS++  + N LP +ELLDDLCSRFVLNVP+ED QSFERILFL+EYA+W+YEDN
Sbjct: 1   MSGLHRSSSSSKNIGNCLPSKELLDDLCSRFVLNVPEEDQQSFERILFLVEYAYWYYEDN 60

Query: 61  SVERNPSLKSLNLKEFTSLLFKSCDVLRPYVAHIDDIFKDFTSYKVRVPVTGAIIMDETF 120
           +VE +P LKSL+LKEFTSLLF SCDVLRPYV HIDDIFKDFTSYK RVPVTGAII+DET+
Sbjct: 61  AVENDPKLKSLSLKEFTSLLFNSCDVLRPYVTHIDDIFKDFTSYKCRVPVTGAIILDETY 120

Query: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE-------------VLEETGFDITNLL 180
           ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRE             VLEETGFD++ LL
Sbjct: 121 ERCLLVKGWKGSSWSFPRGKKSKDEEDHACAIRELSSAILLVNVAFQVLEETGFDVSKLL 180

Query: 181 NKSEYIEVIFGQQRVRLYIIAGVKDDNLFAPQTKKEISEIAWHRLDDLEPASDNVISRGM 240
            + EYIE +F QQRVRLYI+AGV +D +FAP TKKEISEI WHRLD L+PAS+ VI+ G+
Sbjct: 181 KREEYIEFVFRQQRVRLYIVAGVTEDTVFAPLTKKEISEITWHRLDHLQPASNEVITHGV 240

Query: 241 NGLKLYMVAPFLASLRSWISTHHPPVAPNPDVSIRVIINTSLLLISNNRFCF-SNQTVQI 300
           +GLKLYMVAPFL+SL+SWI  H  PVA  P+  ++       L + N R     N T  +
Sbjct: 241 SGLKLYMVAPFLSSLKSWILKHPSPVARRPNKPLKA------LCVWNARTSVGGNGTATV 300

Query: 301 PRHN------LTTLHSLTYINFLPRLTVWK-------AKNSSIGSSSTIIMESQLSKQEV 360
              N       TT+ S +    L R T+          K +    ++T  +ES  +K  V
Sbjct: 301 ESQNRKSELRTTTMESNSRKPELKRTTMESHSTKPELRKGTMESHNTTATVESHNTKPVV 360

Query: 361 DVPIPDTGPGRSFRNFKFDTASILHLYKS 362
           D    D  PG SF NFKF+ + IL   +S
Sbjct: 361 D-HSQDIKPGGSFINFKFNQSVILQALES 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GW312.2e-11960.90mRNA-decapping enzyme subunit 2 OS=Arabidopsis thaliana OX=3702 GN=DCP2 PE=1 SV=... [more]
O138284.0e-5746.30mRNA decapping complex subunit 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
Q9CYC61.3e-5042.24m7GpppN-mRNA hydrolase OS=Mus musculus OX=10090 GN=Dcp2 PE=1 SV=2[more]
Q8IU602.1e-5041.38m7GpppN-mRNA hydrolase OS=Homo sapiens OX=9606 GN=DCP2 PE=1 SV=3[more]
Q5REQ82.1e-5041.38m7GpppN-mRNA hydrolase OS=Pongo abelii OX=9601 GN=DCP2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KHG24.8e-16282.30Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G40... [more]
A0A5D3BM048.2e-16282.58mRNA-decapping enzyme subunit 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C3F48.2e-16282.58mRNA-decapping enzyme subunit 2-like OS=Cucumis melo OX=3656 GN=LOC103496421 PE=... [more]
A0A6J1JIL92.0e-16081.56mRNA-decapping enzyme subunit 2-like OS=Cucurbita maxima OX=3661 GN=LOC111487274... [more]
A0A6J1HJG17.7e-16081.28mRNA-decapping enzyme subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114645... [more]
Match NameE-valueIdentityDescription
XP_038883995.19.3e-16885.96mRNA-decapping enzyme subunit 2-like [Benincasa hispida][more]
XP_004149909.29.9e-16282.30mRNA-decapping enzyme subunit 2 [Cucumis sativus] >KGN47822.1 hypothetical prote... [more]
XP_008456478.11.7e-16182.58PREDICTED: mRNA-decapping enzyme subunit 2-like [Cucumis melo] >KAA0036467.1 mRN... [more]
XP_022990407.14.2e-16081.56mRNA-decapping enzyme subunit 2-like [Cucurbita maxima][more]
XP_022964616.11.6e-15981.28mRNA-decapping enzyme subunit 2-like [Cucurbita moschata] >XP_023540752.1 mRNA-d... [more]
Match NameE-valueIdentityDescription
AT5G13570.11.5e-12060.90decapping 2 [more]
AT5G13570.29.4e-11858.87decapping 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007722mRNA decapping protein 2, Box A domainSMARTSM01125DCP2_2coord: 20..104
e-value: 3.3E-43
score: 159.5
IPR007722mRNA decapping protein 2, Box A domainPFAMPF05026DCP2coord: 21..103
e-value: 2.7E-28
score: 98.1
IPR036189mRNA decapping protein 2, Box A domain superfamilyGENE3D1.10.10.1050Dcp2, box A domaincoord: 22..105
e-value: 7.1E-88
score: 295.6
IPR036189mRNA decapping protein 2, Box A domain superfamilySUPERFAMILY140586Dcp2 domain-likecoord: 15..104
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 106..247
e-value: 7.1E-88
score: 295.6
NoneNo IPR availablePANTHERPTHR23114M7GPPPN-MRNA HYDROLASEcoord: 11..266
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 107..219
e-value: 1.1E-14
score: 54.6
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 105..234
score: 13.088057
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 138..159
IPR044099mRNA decapping enzyme 2 , NUDIX hydrolase domainCDDcd03672Dcp2pcoord: 107..248
e-value: 2.02502E-74
score: 228.276
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 107..247

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G007230.1Lsi08G007230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000290 deadenylation-dependent decapping of nuclear-transcribed mRNA
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050072 m7G(5')pppN diphosphatase activity
molecular_function GO:0030145 manganese ion binding
molecular_function GO:0003723 RNA binding
molecular_function GO:0016787 hydrolase activity