Lsi08G006040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G006040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGFO_IDH_MocA domain-containing protein
Locationchr08: 14321571 .. 14324809 (+)
RNA-Seq ExpressionLsi08G006040
SyntenyLsi08G006040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTCGTTTTGGAATCATCGGCACGGCCGATGTGTCTCGGAAACTTTCTCGAGCTATTGCTCTGGCTCCTAATGCAACTCTCTCAGCAATTGGCAGCCGTTCAATAGAAAAGGCCTCGAAATTTGCGTCGGACAATGGCTTGAGTCCTAAGGTCAAAATCTATGGTTCTTACGAAGCTGTACTCGATGATCCCAAAATCGATGTCGTTTATATTCCCCTTCCGACCAGTCTTCACTTGCATTGGGCTGTACTTGCTGCCCAGAAGAAGAAGCACGTGCTTGTGGAGAAGCCCGTGGCTCTTAATGTAGCTGAGTTGGACAAGATTCTAGAAGCTTGTGAGGCTAATGGAGTGCAGTTCATGGATGGTACTATGTGGATGCACAATCCTCGTACTGCCAAGATGAAGGAATTTCTTTCTGATGCAGACAAGTTTGGTGAGCTCAAATCGGTATGTCATTTTAATTCCAATTAAAATCACTTTTGTCATGTTCTTAATCACTTTGAAACATGCATTTGATTCAGTAGCGGATCCAGAATTTCATGTTAGGAGAGACACAAGGTAGAACTTTAGCCCATACTTTATAGATTTGTGTTTATTTATAGTTTCTAAACTTTAAAAAATGTTTAATAGATCTATGAATTCTCGATTTTGTGTTTAATAGATTCTTAAAATATCAATTATGCGTCCAATAGGTCTCTTACATATTTAAAAATATTATATTAGACATTAAAATTAATTTTGTGCTTAATAAGTACCATTATATATATTTTTAAAATTAATTTATCTATTAACAACAAAATTTAAAACTTAAAGTTTAATTAGACACAAACTGTTTTTTCTTGTCTTATAAAATCGTTAATTTTTAATATTTCTTAGATCTATAAGATGTAAAATTGTAATTTGTAGATTTATTAGACATTTTTAGAGACTATAGTCAAAATTGAAAATTTAGGAATTTATTAGTAACGAATTTCAAAATTTAGGGACTAAATTTATAATTTCACACAATCAAACAATATATATATATATATATATATATATATATATATATATATATATATATATATANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATATATATATATATATATATATATATATATATATATATATATATATATAGATCTTTGAAATTTGAGTTTTGTTTTAATTTGGTCCATAAGTTTCAAGATTTACACTTTTAACATGGATTTTTCACTTAATACTTACTTTCGGTTTTTGGCGTTAATGTCTATTAATTAATTTAAAATAATTATGAAGTATAATTTTAAATTTATTTTAATAGTGATGAAAAATAATGACACTTATTAATTATAATTCTTTTAAATTAATTAATAGACATTACACCAAAGACTAAAAGTGAGTACTTAGTAAAAAAATCAAGGATAAAACTGTAAATCTTAAAACTTAAGAAACAAATTGAAACAACACTCAAATCTCCAGGTTAAAATTGTAACAATTTGAAACCTAGGGACTAATAAATTGAAATCAATCTCAAAACTTAAAAGACTAAATGTATAACATTTTGAAACTTAAGGACCAAATAGAAACTATACCTAAAATCTAGAGACCAAAAAAGTATTTTTCCAAAATTTTATAATAAGTTTCTGAATTCTCAATCATATTTATAAATATTTAAAGTTTCAATTTTGCATGGTCTCGCATATTCAAAAATGTCAATTTTTTCCACTTGAAAATTGTCAAAATTTGAGTGTTACATTAACCATAAAATTTAATTTTGTATCTAATAAACAATGGTTCTATGGTACTTTTTTTAAAAAAATAATTCAATTATTAGTTACAAAATTTAAACTTTTAAGTTAAAAAAATAAATTTTATGTGTAACAAAACAGTTAATTTCTTTAAAAAATAATATTTATGAGACCTATTAAATAAAAATGGAAAAGTTTATTTATTTATTTATTTTTTAGCAGAAAGTTTATATAGATAGTATGACACTGTTAATCATCTTTTGTACCAATGGAGAAGTGGACCCCATGGAATAAGTACCAAAGAAAAAAACAATTTGATGTTATAAATTGCAAATGTAATTTCTATAGAGAGAATACTTAATTAACTTTTATGTTATTTGCTAATTTTTTTCTACATTAATTTTAGTGGTTAGAAGTTGGATTTTTCTTTTGGATTTGGATAATTGATTGATAAGTAAATTGCCCTTTTTTTTTTTTTTCTAAATCGCAGGTACTTAGTATTTCCAGTTATAAAGTTGATTCTAACTTTCTAGCAAATGATATTCGAATAAAACCCGATCTTGATGGACTTGGTGCTTTGGGTGATACCGGATGGTATGGTATTCGTTCAATCCTTTTTGCTGCCGACTTCGAACTGCCTAAAAGAGTGATTGCTTTTCCTAATCCCGTTCTGAATGAATCGGGCGTGGTTTTATCGTGTGGATCCTCTTTATTTTGGGATGATGGAAAAATAGCAACTCTCTATTGCTCTTTCTTATCAAATCATAGCGAGAATATGACTGCCATAGGAACAAATGGGACATTACATGTCACTGATTTCGTTATACCTTACGTAGAGAAAGAGGCTTGTTTCTCCACCGATTCAAAATCTGCATACACTTATCTCGAGACCAGTTGGGAATCACCACCCAACAAACATATCGTCCCTACCGATCTTCCTCAGGAAGTCTATATGGTGAGGGAATTTTCTCATTTAGTTAAAGAGATCAAAGAAAATGGCTCCAAACCTGAGAAAAAATGGTCAACCATCAGTCGAAAGACTCAACTGGTGGTCGATGCAGTGAAGGCATCGCTCGATCGAGGATTCGAACCTGTTGAAGTAGGACAGTGATTCTTGTTTATGAGTGTGATCATGTTCCTTTTCACTTGAAATGGTCTATGTGTGTGATCATGATTCTATCTATATGTATGTTAGTTTTTAAGCTTCCATTGAGGTTGGAATGCTCTGCATTCTTAATTAATATTTCCGGAAGTTCTAAAGAGTTGTGAAAGAAACCATTCCTTGAGAATAATTGCAGGGTTAATTTACATGCATTATTAGATGGTATTAAATAAATATGTCTTCACCTATAAGTTTAAATTTTTGGGCCATTTGGGATTTAATATGATATCAGAGCAAGTTACTTCAGGATGTCCTGGGTTCAAGCCCCT

mRNA sequence

ATGGTTCGTTTTGGAATCATCGGCACGGCCGATGTGTCTCGGAAACTTTCTCGAGCTATTGCTCTGGCTCCTAATGCAACTCTCTCAGCAATTGGCAGCCGTTCAATAGAAAAGGCCTCGAAATTTGCGTCGGACAATGGCTTGAGTCCTAAGGTCAAAATCTATGGTTCTTACGAAGCTGTACTCGATGATCCCAAAATCGATGTCGTTTATATTCCCCTTCCGACCAGTCTTCACTTGCATTGGGCTGTACTTGCTGCCCAGAAGAAGAAGCACGTGCTTGTGGAGAAGCCCGTGGCTCTTAATGTACTTAGTATTTCCAGTTATAAAGTTGATTCTAACTTTCTAGCAAATGATATTCGAATAAAACCCGATCTTGATGGACTTGGTGCTTTGGGTGATACCGGATGGTATGGTATTCGTTCAATCCTTTTTGCTGCCGACTTCGAACTGCCTAAAAGAGTGATTGCTTTTCCTAATCCCGTTCTGAATGAATCGGGCGTGGTTTTATCGTGTGGATCCTCTTTATTTTGGGATGATGGAAAAATAGCAACTCTCTATTGCTCTTTCTTATCAAATCATAGCGAGAATATGACTGCCATAGGAACAAATGGGACATTACATGTCACTGATTTCGTTATACCTTACGTAGAGAAAGAGGCTTGTTTCTCCACCGATTCAAAATCTGCATACACTTATCTCGAGACCAGTTGGGAATCACCACCCAACAAACATATCGTCCCTACCGATCTTCCTCAGGAAGTCTATATGGTGAGGGAATTTTCTCATTTAGTTAAAGAGATCAAAGAAAATGGCTCCAAACCTGAGAAAAAATGGTCAACCATCAGTCGAAAGACTCAACTGGTGGTCGATGCAGTGAAGGCATCGCTCGATCGAGGATTCGAACCTGTTGAAGTAGGACAGTGATTCTTGTTTATGAGTGTGATCATGTTCCTTTTCACTTGAAATGGTCTATGTGTGTGATCATGATTCTATCTATATGTATGTTAGTTTTTAAGCTTCCATTGAGGTTGGAATGCTCTGCATTCTTAATTAATATTTCCGGAAGTTCTAAAGAGTTGTGAAAGAAACCATTCCTTGAGAATAATTGCAGGGTTAATTTACATGCATTATTAGATGGTATTAAATAAATATGTCTTCACCTATAAGTTTAAATTTTTGGGCCATTTGGGATTTAATATGATATCAGAGCAAGTTACTTCAGGATGTCCTGGGTTCAAGCCCCT

Coding sequence (CDS)

ATGGTTCGTTTTGGAATCATCGGCACGGCCGATGTGTCTCGGAAACTTTCTCGAGCTATTGCTCTGGCTCCTAATGCAACTCTCTCAGCAATTGGCAGCCGTTCAATAGAAAAGGCCTCGAAATTTGCGTCGGACAATGGCTTGAGTCCTAAGGTCAAAATCTATGGTTCTTACGAAGCTGTACTCGATGATCCCAAAATCGATGTCGTTTATATTCCCCTTCCGACCAGTCTTCACTTGCATTGGGCTGTACTTGCTGCCCAGAAGAAGAAGCACGTGCTTGTGGAGAAGCCCGTGGCTCTTAATGTACTTAGTATTTCCAGTTATAAAGTTGATTCTAACTTTCTAGCAAATGATATTCGAATAAAACCCGATCTTGATGGACTTGGTGCTTTGGGTGATACCGGATGGTATGGTATTCGTTCAATCCTTTTTGCTGCCGACTTCGAACTGCCTAAAAGAGTGATTGCTTTTCCTAATCCCGTTCTGAATGAATCGGGCGTGGTTTTATCGTGTGGATCCTCTTTATTTTGGGATGATGGAAAAATAGCAACTCTCTATTGCTCTTTCTTATCAAATCATAGCGAGAATATGACTGCCATAGGAACAAATGGGACATTACATGTCACTGATTTCGTTATACCTTACGTAGAGAAAGAGGCTTGTTTCTCCACCGATTCAAAATCTGCATACACTTATCTCGAGACCAGTTGGGAATCACCACCCAACAAACATATCGTCCCTACCGATCTTCCTCAGGAAGTCTATATGGTGAGGGAATTTTCTCATTTAGTTAAAGAGATCAAAGAAAATGGCTCCAAACCTGAGAAAAAATGGTCAACCATCAGTCGAAAGACTCAACTGGTGGTCGATGCAGTGAAGGCATCGCTCGATCGAGGATTCGAACCTGTTGAAGTAGGACAGTGA

Protein sequence

MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEAVLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVLSISSYKVDSNFLANDIRIKPDLDGLGALGDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLSNHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLPQEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVGQ
Homology
BLAST of Lsi08G006040 vs. ExPASy Swiss-Prot
Match: Q9SZ83 (Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g09670 PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.4e-100
Identity = 183/353 (51.84%), Postives = 239/353 (67.71%), Query Frame = 0

Query: 2   VRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEAV 61
           +R G++G AD++RK+SRAI LAPNAT+S + SRS+EKA  FA+ N      KI+GSYE++
Sbjct: 7   IRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSYESL 66

Query: 62  LDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL----------------- 121
           L+DP+ID +Y+PLPTSLH+ WA+ AA+K KH+L+EKPVA+NV                  
Sbjct: 67  LEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGVQIM 126

Query: 122 -------------------------------SISSYKVDSNFLANDIRIKPDLDGLGALG 181
                                          S  S+  D +FL NDIR+KP LDGLGALG
Sbjct: 127 DGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLGALG 186

Query: 182 DTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLSN 241
           D GWY IR+ L A +FELPK V AFP  VLNE+GV+LSCG+SL W+DG+ AT+YCSFL+N
Sbjct: 187 DAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSFLAN 246

Query: 242 HSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLPQ 301
            +  +TAIGT GTL V DF+IPY E EA F+T +K+ +  L T+W SPP++H V T+LPQ
Sbjct: 247 LTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTELPQ 306

Query: 302 EVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEV 307
           E  MVREF+ LV EIK NG+KP+  W +ISRKTQLVVDAVK S+D+ ++ + +
Sbjct: 307 EACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of Lsi08G006040 vs. ExPASy Swiss-Prot
Match: P49305 (Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.6e-17
Identity = 50/108 (46.30%), Postives = 73/108 (67.59%), Query Frame = 0

Query: 1   MVRFGIIGTADVS-RKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYE 60
           +VRFGIIGTA ++  K+  ++  A    + AI SR +++A   A+  G+    + YGSY+
Sbjct: 3   LVRFGIIGTAAIAVEKVIPSMLSAEGLEVVAIASRDLDRARAAATRFGIG---RSYGSYD 62

Query: 61  AVLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVLSIS 108
            +L DP+I+ VYIPLP  LH+HWA+ AA+  KHVL EKP+AL+V  +S
Sbjct: 63  EILADPEIEAVYIPLPNHLHVHWAIRAAEAGKHVLCEKPLALDVEELS 107

BLAST of Lsi08G006040 vs. ExPASy Swiss-Prot
Match: D4GP30 (D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) OX=309800 GN=HVO_B0029 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.5e-15
Identity = 82/272 (30.15%), Postives = 111/272 (40.81%), Query Frame = 0

Query: 4   FGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEAVLD 63
           FGI+GTA +  K       A     +AI SR   +AS  A + G+      YGSYEA+L 
Sbjct: 3   FGILGTAGIGVKSVIPAVQASEHEAAAIASRDEARASAVADELGIP---TAYGSYEALLA 62

Query: 64  DPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPV-----------------ALNVLSI 123
           D  +D VYIPLP  LH  W   AA +  HVL EKP+                  + ++  
Sbjct: 63  DDSLDAVYIPLPNGLHADWVRAAADRGLHVLCEKPLTASADETAAVFDYCEDAGVTLMEA 122

Query: 124 SSYK---------------------VDSNFL------ANDIRIKPDLDGLGALGDTGWYG 183
             Y+                     V SNF       A+DIRI PDL G G++ D G Y 
Sbjct: 123 FMYRFHPLTERAAELVASELGAVVSVTSNFSFRLPDGADDIRIDPDLAG-GSVMDVGCYA 182

Query: 184 IRSI-LFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLSNHSENM 229
           + +  LF      P RV A       + GV       L +D G  A +  SF +  ++  
Sbjct: 183 VSAARLFLG---TPDRVYATTTDT-RDCGVDTRMSGVLEYDSGATARVESSFDTPETQYY 242

BLAST of Lsi08G006040 vs. ExPASy Swiss-Prot
Match: Q9ALN5 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Saccharopolyspora spinosa OX=60894 GN=spnN PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.5e-10
Identity = 77/291 (26.46%), Postives = 115/291 (39.52%), Query Frame = 0

Query: 2   VRFGIIGTADVS-RKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 61
           VR G++G A  + R++  A+       + A+ SR   KA +FA+      + +    Y+ 
Sbjct: 5   VRIGVLGCASFAWRRMLPAMCDVAETEVVAVASRDPAKAERFAA----RFECEAVLGYQR 64

Query: 62  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVAL---------------NVLS 121
           +L+ P ID VY+PLP  +H  W   A +  KHVL EKP+                 N+L 
Sbjct: 65  LLERPDIDAVYVPLPPGMHAEWIGKALEADKHVLAEKPLTTTASDTARLVGLARRKNLLL 124

Query: 122 ISSY--------KVDSNFLAN---------------------DIRIKPDLDGLGALGDTG 181
             +Y         V  + L +                     DIR + +L G GAL D G
Sbjct: 125 RENYLFLHHGRHDVVRDLLQSGEIGELREFTAVFGIPPLPDTDIRYRTELGG-GALLDIG 184

Query: 182 WYGIRSILFAADFEL-PKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLSNHS 241
            Y  R+   A  F L P  V+   +    ESGV LS    L  + G +A L   F+ ++ 
Sbjct: 185 VYPARA---ARHFLLGPLTVLGASSHEAQESGVDLSGSVLLQSEGGTVAHLGYGFVHHYR 244

Query: 242 ENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHI 247
                 G+ G + V     P  E +A    + K     L    E    K +
Sbjct: 245 SAYELWGSRGRIVVDRAFTPPAEWQAVIRIERKGVVDELSLPAEDQVRKAV 287

BLAST of Lsi08G006040 vs. ExPASy Swiss-Prot
Match: Q9RR32 (dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces antibioticus OX=1890 GN=oleW PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.5e-10
Identity = 36/99 (36.36%), Postives = 60/99 (60.61%), Query Frame = 0

Query: 2   VRFGIIGTADVS-RKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 61
           +RFG++G AD++ R+   A+   P+ T+ A+ SR   +A++FA+  G     +    ++A
Sbjct: 6   LRFGVLGAADIALRRTVPALLAHPDVTVVAVSSRDTARAARFAAAFG----CEAVPGHQA 65

Query: 62  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPV 100
           +LD   ID +Y+PLP  +H  W   A  + +HVLVEKP+
Sbjct: 66  LLDRDDIDALYVPLPVMVHTPWVEAALLRGRHVLVEKPL 100

BLAST of Lsi08G006040 vs. ExPASy TrEMBL
Match: A0A6J1HFT3 (uncharacterized oxidoreductase At4g09670 OS=Cucurbita moschata OX=3662 GN=LOC111463907 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.0e-131
Identity = 246/355 (69.30%), Postives = 277/355 (78.03%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           +VRFGIIG A+++RK+SRAIALAPNATLSAIGSRS+EKASKFA DNG SP+VKIYGSYEA
Sbjct: 5   IVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  D++FLANDIR+K DLDGLGAL
Sbjct: 125 MDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA+FELPKRVIAFPNPVLNESGV+LSCG+SLFWDDGKIAT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLHV DF+IPY EKEA F T+SKS +T L T WE  P+KHIVPTDLP
Sbjct: 245 NLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVG 308
           QE +MVREFSHLVKEIK NGSKPEKKW  ISRKTQLV+DAVKASLD GF+PVEVG
Sbjct: 305 QEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of Lsi08G006040 vs. ExPASy TrEMBL
Match: A0A6J1JMT8 (uncharacterized oxidoreductase At4g09670 OS=Cucurbita maxima OX=3661 GN=LOC111487263 PE=4 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.3e-131
Identity = 246/355 (69.30%), Postives = 276/355 (77.75%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           +VRFGIIG A+++RK+SRAIALAPNATLSAIGSRS+EKASKFA DNG SP+VKIYGSYEA
Sbjct: 5   IVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  D++FLANDIR+K DLDGLGAL
Sbjct: 125 MDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA FELPKRVIAFPNPVLNESGV+LSCG+SLFWDDGKIAT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAASFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLHV DF+IPY EKEA F T+SKS +T L T WE  P+KHIVPTDLP
Sbjct: 245 NLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVG 308
           QE +MVREFSHLVKEIK NGSKPEKKW  ISRKTQLV+DAVKASLD GF+PVEVG
Sbjct: 305 QEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of Lsi08G006040 vs. ExPASy TrEMBL
Match: A0A6J1BYS0 (uncharacterized oxidoreductase At4g09670-like OS=Momordica charantia OX=3673 GN=LOC111006693 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.7e-131
Identity = 245/355 (69.01%), Postives = 276/355 (77.75%), Query Frame = 0

Query: 2   VRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEAV 61
           VRFGIIG A+++RK++RAIALAPNATLSAIGSR++EKASKFASDNG +P+VKIYGSYEAV
Sbjct: 6   VRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSYEAV 65

Query: 62  LDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL----------------- 121
           LDDP++D VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                  
Sbjct: 66  LDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQFM 125

Query: 122 -------------------------------SISSYKVDSNFLANDIRIKPDLDGLGALG 181
                                          SI S+  D++FLANDIR+K DLDGLGALG
Sbjct: 126 DGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLGALG 185

Query: 182 DTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLSN 241
           D GWY IRSILFAADFELPKRVIA PNPVLNESGV+LSCG+SL+WDDGK+AT +CSFLSN
Sbjct: 186 DAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCSFLSN 245

Query: 242 HSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLPQ 301
            + +MTAIGTNG LHV DF+IPY EKEA F+T SKS +T L T WE  P+KHIVPTDLPQ
Sbjct: 246 LTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVPTDLPQ 305

Query: 302 EVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVGQ 309
           E  MVREFSHLVKEIK NGSKPEKKW TISRKTQLVVDAVKASLDRG EPVEVG+
Sbjct: 306 EARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK 360

BLAST of Lsi08G006040 vs. ExPASy TrEMBL
Match: A0A0A0KB74 (GFO_IDH_MocA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G052640 PE=4 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.6e-129
Identity = 243/356 (68.26%), Postives = 276/356 (77.53%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           ++RFGIIG AD++RK+SRAIALAPNATLSAIGSRS+EKASKFASDNG SP VKIYGSYEA
Sbjct: 5   IIRFGIIGAADIARKVSRAIALAPNATLSAIGSRSLEKASKFASDNGFSPHVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  DS+FLANDIR+KPDLDGLGAL
Sbjct: 125 MDGTMWMHNPRTAKMKEFLSDADKFGQLKAVNSVFSFAADSDFLANDIRVKPDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA++ELPKRVIA PNPVLNE GV+LSCG SL W+DGK+AT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAANYELPKRVIALPNPVLNELGVILSCGCSLLWEDGKVATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLH+ DF+IPYVEKEA FST SKS +  L T W+  P+KHIVPTDLP
Sbjct: 245 NLTMDVTAIGTNGTLHLNDFIIPYVEKEAYFSTCSKSGFNDLVTGWQPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVGQ 309
           QE +MVREFSHLVKEIK NGSKPEKKW TI+RKTQLV+DAVKASLDRG EPVE+GQ
Sbjct: 305 QEAHMVREFSHLVKEIKLNGSKPEKKWPTITRKTQLVLDAVKASLDRG-EPVELGQ 359

BLAST of Lsi08G006040 vs. ExPASy TrEMBL
Match: A0A5A7SM23 (Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G00420 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 6.1e-129
Identity = 239/355 (67.32%), Postives = 275/355 (77.46%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           ++RFGIIG AD++RK+SRAIALAPNATLSAIGSRS+EKASKFASDNG SP VKIYGSYEA
Sbjct: 5   IIRFGIIGAADIARKVSRAIALAPNATLSAIGSRSLEKASKFASDNGFSPHVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  D++FLANDIR+KPDLDGLGAL
Sbjct: 125 MDGTMWMHNPRTAKMKEFLFDADKFGQLKAVNSVFSFAADADFLANDIRVKPDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA++ELPKRVIA PNPVLNE GV+LSCG SL W+DGK+AT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAANYELPKRVIALPNPVLNELGVILSCGCSLLWEDGKVATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLH+ DF+IPYVEKEA FST SKS +T L T W+  P+KHIVPTDLP
Sbjct: 245 NLTMDVTAIGTNGTLHLNDFIIPYVEKEAYFSTCSKSGFTELVTGWQPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVG 308
           QE +MVREFSHLVKEIK NG+KPE+KW TI+RKTQLV+DAVKASLD GF PVE+G
Sbjct: 305 QEAHMVREFSHLVKEIKLNGAKPEEKWPTITRKTQLVLDAVKASLDGGFVPVELG 359

BLAST of Lsi08G006040 vs. NCBI nr
Match: XP_022963640.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >XP_022963647.1 uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >KAG7032898.1 putative oxidoreductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 479.6 bits (1233), Expect = 2.1e-131
Identity = 246/355 (69.30%), Postives = 277/355 (78.03%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           +VRFGIIG A+++RK+SRAIALAPNATLSAIGSRS+EKASKFA DNG SP+VKIYGSYEA
Sbjct: 5   IVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  D++FLANDIR+K DLDGLGAL
Sbjct: 125 MDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA+FELPKRVIAFPNPVLNESGV+LSCG+SLFWDDGKIAT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLHV DF+IPY EKEA F T+SKS +T L T WE  P+KHIVPTDLP
Sbjct: 245 NLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVG 308
           QE +MVREFSHLVKEIK NGSKPEKKW  ISRKTQLV+DAVKASLD GF+PVEVG
Sbjct: 305 QEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of Lsi08G006040 vs. NCBI nr
Match: XP_022990391.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita maxima] >XP_022990392.1 uncharacterized oxidoreductase At4g09670 [Cucurbita maxima])

HSP 1 Score: 479.2 bits (1232), Expect = 2.7e-131
Identity = 246/355 (69.30%), Postives = 276/355 (77.75%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           +VRFGIIG A+++RK+SRAIALAPNATLSAIGSRS+EKASKFA DNG SP+VKIYGSYEA
Sbjct: 5   IVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  D++FLANDIR+K DLDGLGAL
Sbjct: 125 MDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA FELPKRVIAFPNPVLNESGV+LSCG+SLFWDDGKIAT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAASFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLHV DF+IPY EKEA F T+SKS +T L T WE  P+KHIVPTDLP
Sbjct: 245 NLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVG 308
           QE +MVREFSHLVKEIK NGSKPEKKW  ISRKTQLV+DAVKASLD GF+PVEVG
Sbjct: 305 QEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKASLDGGFKPVEVG 359

BLAST of Lsi08G006040 vs. NCBI nr
Match: XP_022134449.1 (uncharacterized oxidoreductase At4g09670-like [Momordica charantia])

HSP 1 Score: 478.8 bits (1231), Expect = 3.6e-131
Identity = 245/355 (69.01%), Postives = 276/355 (77.75%), Query Frame = 0

Query: 2   VRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEAV 61
           VRFGIIG A+++RK++RAIALAPNATLSAIGSR++EKASKFASDNG +P+VKIYGSYEAV
Sbjct: 6   VRFGIIGCAEIARKVARAIALAPNATLSAIGSRTLEKASKFASDNGFTPRVKIYGSYEAV 65

Query: 62  LDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL----------------- 121
           LDDP++D VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                  
Sbjct: 66  LDDPEVDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVAELDKILEACESNGVQFM 125

Query: 122 -------------------------------SISSYKVDSNFLANDIRIKPDLDGLGALG 181
                                          SI S+  D++FLANDIR+K DLDGLGALG
Sbjct: 126 DGTMWMHNPRTAKMKEFLSDADQFGQLKSVHSIFSFTADADFLANDIRVKSDLDGLGALG 185

Query: 182 DTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLSN 241
           D GWY IRSILFAADFELPKRVIA PNPVLNESGV+LSCG+SL+WDDGK+AT +CSFLSN
Sbjct: 186 DAGWYCIRSILFAADFELPKRVIALPNPVLNESGVILSCGASLYWDDGKVATFHCSFLSN 245

Query: 242 HSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLPQ 301
            + +MTAIGTNG LHV DF+IPY EKEA F+T SKS +T L T WE  P+KHIVPTDLPQ
Sbjct: 246 LTMDMTAIGTNGALHVNDFIIPYGEKEAHFTTGSKSGFTELVTGWEPLPSKHIVPTDLPQ 305

Query: 302 EVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVGQ 309
           E  MVREFSHLVKEIK NGSKPEKKW TISRKTQLVVDAVKASLDRG EPVEVG+
Sbjct: 306 EARMVREFSHLVKEIKANGSKPEKKWPTISRKTQLVVDAVKASLDRGSEPVEVGK 360

BLAST of Lsi08G006040 vs. NCBI nr
Match: XP_023540091.1 (uncharacterized oxidoreductase At4g09670 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.0 bits (1229), Expect = 6.1e-131
Identity = 245/355 (69.01%), Postives = 276/355 (77.75%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           +VRFGIIG A+++RK+SRAIALAPNATLSAIGSRS+EKASKFA DNG SP+VKIYGSYEA
Sbjct: 5   IVRFGIIGAAEIARKVSRAIALAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  D++FLANDIR+K DLDGLGAL
Sbjct: 125 MDGTMWMHNPRTALMKEFLSDADQFGQLKSVNSVFSFAADADFLANDIRVKSDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA+FELPKRVIAFPNPVLNESGV+LSCG+SLFWDDGKIAT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLHV DF+IPY EKEA F T+SKS +T L T WE  P+KHIVPTDLP
Sbjct: 245 NLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVG 308
           QE +MVREFSHLVKEIK NGSKPEKKW  ISRKTQLV+DAVK SLD GF+PVEVG
Sbjct: 305 QEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKVSLDGGFKPVEVG 359

BLAST of Lsi08G006040 vs. NCBI nr
Match: KAG6602218.1 (putative oxidoreductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 477.2 bits (1227), Expect = 1.0e-130
Identity = 244/355 (68.73%), Postives = 276/355 (77.75%), Query Frame = 0

Query: 1   MVRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEA 60
           +VRFGIIG A+++RK+SRAIA+APNATLSAIGSRS+EKASKFA DNG SP+VKIYGSYEA
Sbjct: 5   IVRFGIIGAAEIARKVSRAIAIAPNATLSAIGSRSVEKASKFAVDNGFSPQVKIYGSYEA 64

Query: 61  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL---------------- 120
           VLDDP+ID VY+PLPTSLHL WAVLAA+KKKH+L+EKPVALNV                 
Sbjct: 65  VLDDPEIDAVYVPLPTSLHLRWAVLAAEKKKHLLLEKPVALNVSEFDKILEACEANGVQF 124

Query: 121 --------------------------------SISSYKVDSNFLANDIRIKPDLDGLGAL 180
                                           S+ S+  D++FLANDIR+K DLDGLGAL
Sbjct: 125 MDGTMWMHNPRTALMKEFLSDAEQFGQLKSVNSVFSFAADADFLANDIRVKSDLDGLGAL 184

Query: 181 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 240
           GD GWY IRSILFAA+FELPKRVIAFPNPVLNESGV+LSCG+SLFWDDGKIAT +CSFLS
Sbjct: 185 GDAGWYCIRSILFAANFELPKRVIAFPNPVLNESGVILSCGASLFWDDGKIATFHCSFLS 244

Query: 241 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 300
           N + ++TAIGTNGTLHV DF+IPY EKEA F T+SKS +T L T WE  P+KHIVPTDLP
Sbjct: 245 NLTMDITAIGTNGTLHVNDFIIPYGEKEAYFCTNSKSGFTELVTGWEPLPSKHIVPTDLP 304

Query: 301 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEVG 308
           QE +MVREFSHLVKEIK NGSKPEKKW  ISRKTQLV+DAVK SLD GF+PVEVG
Sbjct: 305 QEAHMVREFSHLVKEIKANGSKPEKKWPNISRKTQLVLDAVKVSLDGGFKPVEVG 359

BLAST of Lsi08G006040 vs. TAIR 10
Match: AT4G09670.1 (Oxidoreductase family protein )

HSP 1 Score: 365.9 bits (938), Expect = 3.1e-101
Identity = 183/353 (51.84%), Postives = 239/353 (67.71%), Query Frame = 0

Query: 2   VRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPKVKIYGSYEAV 61
           +R G++G AD++RK+SRAI LAPNAT+S + SRS+EKA  FA+ N      KI+GSYE++
Sbjct: 7   IRIGVMGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSYESL 66

Query: 62  LDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVL----------------- 121
           L+DP+ID +Y+PLPTSLH+ WA+ AA+K KH+L+EKPVA+NV                  
Sbjct: 67  LEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKPVAMNVTEFDKIVDACEANGVQIM 126

Query: 122 -------------------------------SISSYKVDSNFLANDIRIKPDLDGLGALG 181
                                          S  S+  D +FL NDIR+KP LDGLGALG
Sbjct: 127 DGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLGALG 186

Query: 182 DTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLSN 241
           D GWY IR+ L A +FELPK V AFP  VLNE+GV+LSCG+SL W+DG+ AT+YCSFL+N
Sbjct: 187 DAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSFLAN 246

Query: 242 HSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLPQ 301
            +  +TAIGT GTL V DF+IPY E EA F+T +K+ +  L T+W SPP++H V T+LPQ
Sbjct: 247 LTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTELPQ 306

Query: 302 EVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEV 307
           E  MVREF+ LV EIK NG+KP+  W +ISRKTQLVVDAVK S+D+ ++ + +
Sbjct: 307 EACMVREFARLVGEIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISL 359

BLAST of Lsi08G006040 vs. TAIR 10
Match: AT1G34200.1 (Glyceraldehyde-3-phosphate dehydrogenase-like family protein )

HSP 1 Score: 290.8 bits (743), Expect = 1.3e-78
Identity = 159/352 (45.17%), Postives = 206/352 (58.52%), Query Frame = 0

Query: 2   VRFGIIGTADVSRKLSRAIALAPNATLSAIG-SRSIEKASKFASDNGLSPKVKIYGSYEA 61
           +R G++   +  RKLSRAI L+PNAT+SAI  + SIE+A  FA  N   P  K++ SYE+
Sbjct: 7   IRIGLLSCTNKVRKLSRAINLSPNATISAIATTSSIEEAKSFAKSNNFPPNTKLHSSYES 66

Query: 62  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALN------------------ 121
           +L+DP +D VY P+PT LH+ WA LAA K KH+L++KPVALN                  
Sbjct: 67  LLEDPDVDAVYFPIPTRLHVEWATLAAIKGKHILLDKPVALNVAEFDQIVEACEVNGVQF 126

Query: 122 ------------------------------VLSISSYKVDSNFLANDIRIKPDLDGLGAL 181
                                         V S  S+    +FL +DIR+KP LDGLGAL
Sbjct: 127 MDGTQWMHSPRTDKIKEFVNDLESFGQIKSVYSCFSFAASDDFLKHDIRVKPGLDGLGAL 186

Query: 182 GDTGWYGIRSILFAADFELPKRVIAFPNPVLNESGVVLSCGSSLFWDDGKIATLYCSFLS 241
           GD GWY I++IL   +F+LPK V A    VLN++G+VLSCG+   W+DG  AT+YCSFL+
Sbjct: 187 GDAGWYTIQAILLVNNFKLPKTVTALSGSVLNDAGIVLSCGALFDWEDGVNATIYCSFLA 246

Query: 242 NHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVPTDLP 301
           N +  +TAIGT G+L V DFVIPY+E EA F+T                 ++H V T+L 
Sbjct: 247 NLTMEITAIGTKGSLRVHDFVIPYMETEAVFTTSGGGCV-----------SEHKVMTELS 306

Query: 302 QEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPV 305
           QE  MV EF+ LV EIK  G+KP+  W   SR TQL+VDAVK S+D   EPV
Sbjct: 307 QEACMVMEFARLVGEIKNRGAKPDGFWMRFSRNTQLLVDAVKESIDNNLEPV 347

BLAST of Lsi08G006040 vs. TAIR 10
Match: AT1G66130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 194.9 bits (494), Expect = 9.5e-50
Identity = 126/358 (35.20%), Postives = 185/358 (51.68%), Query Frame = 0

Query: 2   VRFGIIGTADVSRKLSRAIALAPNATLSAIGSRSIEKASKFASDNGLSPK-VKIYGSYEA 61
           VRFG++G    + K  R +  + NA + AI   S+E A  FA+ N LSP+ V IYGSYE 
Sbjct: 7   VRFGLLGCIRFASKFVRTVTESSNAVIIAISDPSLETAKTFATGNNLSPETVTIYGSYEE 66

Query: 62  VLDDPKIDVVYIPLPTSLHLHWAVLAAQKKKHVLVEKPVALNVLSIS------------- 121
           +L+D  +D VY+ +P +    WAV AA+KKKHVLVEKP A +   +              
Sbjct: 67  LLNDANVDAVYLTMPVTQRARWAVTAAEKKKHVLVEKPPAQDATELEKIVEACEYNGVQF 126

Query: 122 -----------SYKV-DSNF---LANDIR------------------IKPDLDGLGALGD 181
                      + K+ D+ F   L  D+R                   K  +   GA+G+
Sbjct: 127 MDGTIWLHHQRTVKIRDTMFDSGLLGDVRHMYSTMTTPVPEQVLERLTKEAMGLAGAIGE 186

Query: 182 TGWYGIRSILFAADFELPKRVIAFPNPV-LNESGVVLSCGSSLFWDDGKIAT--LYCSFL 241
            GWY I + L+A  +++P  V A P+ V  N  G +LSC +SL +      T  ++CSFL
Sbjct: 187 LGWYPIGAALWAMSYQMPISVRALPSSVSTNSVGTILSCTASLQFGSTATTTAIVHCSFL 246

Query: 242 SNHSENMTAIGTNGTLHVTDFVIPYVEKEACFSTDSKSAYTYLETSWESPPNKHIVP--- 301
           S  S ++   G+ G++ + D+VIPY E +A F   S + +  +   W   P +  V    
Sbjct: 247 SQLSTDLAISGSKGSIQMNDYVIPYKEDKAWFEYTSGAKFVDMHIGWNVTPERVTVDCSG 306

Query: 302 TDLPQEVYMVREFSHLVKEIKENGSKPEKKWSTISRKTQLVVDAVKASLDRGFEPVEV 307
           T   QE  M+REF+ LV+ IK    + +++W  IS+KTQL+VDAVK S+D G E V +
Sbjct: 307 TPETQEAMMLREFTRLVEGIKRGDLEADRRWPDISKKTQLIVDAVKKSVDIGCEVVHL 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ834.4e-10051.84Uncharacterized oxidoreductase At4g09670 OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
P493051.6e-1746.30Uncharacterized oxidoreductase ORF334 OS=Rhizobium meliloti OX=382 PE=3 SV=1[more]
D4GP301.5e-1530.15D-xylose 1-dehydrogenase (NADP(+)) 2 OS=Haloferax volcanii (strain ATCC 29605 / ... [more]
Q9ALN51.5e-1026.46dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Saccharopolyspora ... [more]
Q9RR322.5e-1036.36dTDP-3,4-didehydro-2,6-dideoxy-alpha-D-glucose 3-reductase OS=Streptomyces antib... [more]
Match NameE-valueIdentityDescription
A0A6J1HFT31.0e-13169.30uncharacterized oxidoreductase At4g09670 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1JMT81.3e-13169.30uncharacterized oxidoreductase At4g09670 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1BYS01.7e-13169.01uncharacterized oxidoreductase At4g09670-like OS=Momordica charantia OX=3673 GN=... [more]
A0A0A0KB741.6e-12968.26GFO_IDH_MocA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G05264... [more]
A0A5A7SM236.1e-12967.32Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
XP_022963640.12.1e-13169.30uncharacterized oxidoreductase At4g09670 [Cucurbita moschata] >XP_022963647.1 un... [more]
XP_022990391.12.7e-13169.30uncharacterized oxidoreductase At4g09670 [Cucurbita maxima] >XP_022990392.1 unch... [more]
XP_022134449.13.6e-13169.01uncharacterized oxidoreductase At4g09670-like [Momordica charantia][more]
XP_023540091.16.1e-13169.01uncharacterized oxidoreductase At4g09670 [Cucurbita pepo subsp. pepo][more]
KAG6602218.11.0e-13068.73putative oxidoreductase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G09670.13.1e-10151.84Oxidoreductase family protein [more]
AT1G34200.11.3e-7845.17Glyceraldehyde-3-phosphate dehydrogenase-like family protein [more]
AT1G66130.19.5e-5035.20NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.360.10Dihydrodipicolinate Reductase; domain 2coord: 106..308
e-value: 1.2E-55
score: 190.5
NoneNo IPR availableGENE3D3.40.50.720coord: 1..105
e-value: 7.8E-28
score: 99.4
NoneNo IPR availablePANTHERPTHR46368FAMILY NOT NAMEDcoord: 2..105
NoneNo IPR availablePANTHERPTHR46368FAMILY NOT NAMEDcoord: 109..306
NoneNo IPR availablePANTHERPTHR46368:SF13SUBFAMILY NOT NAMEDcoord: 109..306
coord: 2..105
NoneNo IPR availableSUPERFAMILY55347Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domaincoord: 108..266
IPR000683Gfo/Idh/MocA-like oxidoreductase, N-terminalPFAMPF01408GFO_IDH_MocAcoord: 2..103
e-value: 7.3E-15
score: 55.8
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 2..103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G006040.1Lsi08G006040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000166 nucleotide binding