Lsi08G005630 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G005630
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212
Location	chr08: 13824850 .. 13828778 (+)
RNA-Seq Expression	Lsi08G005630
Synteny	Lsi08G005630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAATATTAAAAAAATAAAAAATAAAAATCTTATCTTCATCCGTGGCGAACTATCCGCGAGGCGCGAACACTCGAACAGTACACTGAAATTCTCGTTAATGGATTCCAATCTCCTGCAATTATCCCACACACTATCACTCAAATCCACATTCCTCAACGCAATTCGAAGATCCCCTTCCATCTCTTCAATCTCCATCAGAAGAAATTTTCAGTTCCAGAGGCACTTCAAGCTGGAGAGCAGTAAAAATGGTGGCAGAGAAACCCAACGGGATTGTGCTTTCGACGAAGTAGCATTCGAGGCCGAGAGATCTCGCCTCGACGCCCAAGCTGTAAAATCCATGGCCGAAGCTTCAATTAGGGACACTGAAGGCGCTTCCGACGACGACCCGAAAGCTTGGAAGTGGATCATCCGAAAACGAGTTTGGGATTTCATGGAGTCACAAAATATCGCGGCAAATCCCCGTCCCGTTCACCATCGAATCCCTAATTTCCTTGGCGCCATGGAAGCTGCCAATAGAGTGAGCCTCCCCAATTCCTGAATTGTTTTCTTGGAAAGAAAATGTTTGAGTCGATCTTTTCAGTATTGGAAATTTTGATTCCTTCCGCGTATACCTGATGAAATGGAGTTGTATCAGTTGTGCGATTTGGAAGTATTCAGGGATTCACAGTGCGTGAAGGTTAATCCGGATTCTCCTCAGAAGGGTGTGAGGCTTCTTACACTTACGGGTCAGTTCTGCTGATACTTTTATGATTTATCCTGCTGTTCTTCTCGTTTGGTTGTTTGTTTTCTTTAGATTGTCTACCTACCATCGTCAAGAGACTATGTTTCGAGCTGTTAATGAATTCGAACACTCAATGCTAGCGAACAATACTATTTCGATATGATTTAGATTGGCATTTTTTATGTCATATCTTCTCTGGTGACTGAATGATAGTCTGGTCAATTGCAAGTCCGGTCTCTGAACTTGCGTGTGTCTATTTGGTTCGTGAAGTTTCAAAAGTGTCTAATAATCTTGAAGTTTCAATTGTGTTCAATAACCTGAACTTTAAAAACGGTTTAATGGATCTCCAAAATTTTAATTTCATGTCCAATATGTTTTTGACTTGCTCGATATTTCAAAAATTAATGAGTCTACCCGACACCAAATTGAAAATTTAAAGTTCTATCGTACACAAATTTCAAATTCGTATCTAATGGATCAATTATTTTTTGTGAAAATTTTGAATACATCATAGATTTATTAGACACAAAATAAAAAGTTTTTAGACCTAGTGACCTATTAGATACATAATTGAACACTTTTGAAAATTCAAAAGACTAAACACACGCAAACCTAAAAGTTCAGGTACTAAACTTCTAATTTAAACTTAATGTTTCTTTCTTTTCTTGAAGAGACTTTTGTCTGTGCATGATTCAACATCTGTGTACCTATATCTGCAAAAATATGATAATTGATTAGTAAATAAACGATGGAGATAATTCTTTTATTTTCTGGATAACCCTGTAGTACTTATCAAGAAGAGAGTTCACTCAAGGATGTTAAACCTTACAAAGCCTTGTGCAAAATTGTATGGCAAAAGGCCTGCATGAGTGATGTGAGGAGGGGAAACTTCAAAATATGTCAACTGGTGATGTCTTAGTTTATAACTAAAGCTAGGAAAGACGAGATCTACTGAAATTATGAATGTCTAATGGTAATTTGGTGGTTGGCTGTCTCAACGTCGACATTGTCAGATTTAGTTACAATTTTCTGGTGGTTAAATCATACAGTGTAAGCTTCGACTGAATCGTGAACATCATTTCACGACTTAGCGAGTTGTATGTAACGTCTTTGATTCTGAAAAAGCATGAAAGCTTTCTCATGTTAAATCACAAACTAAATGGTATTCTCATATGATCAGGTGGCAAAAAACTGTTAACACCTCAGCCTCGGTTAAGAACAGGGTTTTTCTCCATAGTCGAATCAGGGATGTTGACTCCGGCTACCATCAACGAGGCCTGCACTTCTGTTGGGGTAGCAAAGTATGGAAAGCCTATTGGACTGGATGAAAAGATCAAAGTTGATCTGATCATCATTGGTTCTGTTGCTGTTGACCCCAGAACGGGTGCTCGGCTTGGCAAGGGAGAGGTAAAAGTGTTACTGTTGGCTTTACCTTTTACATCGAAAATGGCTGGTTATAAAACATATTTGCTCAGAAGATTAGTCCTCAGTCTTCAGACATCCTCTCTTAGTATCTTCCCACATCTTCTCTACAGCCATCTATTTGTTTGCACATAACTTACATCTATGGCTCATTTCTTGCCTTCTTTGCCACTTTGAAAATGGAATGGATTGATTAAGGAACTCTTTAGATTGACTTTCAAAGTTCTTAGAAAGGTGTTTTTAAGTGCAAATGAAGTGTTTATAAATACTTAGAAAGTCAATTCAAATAGACCTCAACAAGGGAAATAGTATGTTTGATTCTAACTATGCTTCTTTAGGCCACTTTATAAAAATACATCCAATAGGTTTTTGAACTTTAAAAAGTGCCTAATCGGTCTTTAGACTTTCAATTTTGTGTCTAATAGATTTTGACTTATTTGATATTTTTATAATTCACAGCTCTAGTAGACACAAAATTGAATGTAAACTTAAAAGCTTATAAACTAAACTTAAAATTTACTCTATAAAATGCAGGGATTTGCAGAACTAGAATATGGAATGCTGCGGTACATGGGAGCCATTGATGACTCGACTCCAATAGTTACTTCTGGTTGTGTAACTACTCTGCTTCTATCTTATATTGTAGTTTCTCATTCTTTTCATTATTTTCAGCAACAATTTCTTGTGTAACATAATTTAACAGGTGGAGATAAATTTCAGTGCTTAATATCGAAACGTTCTTTACTATCAACTTAGATCGTGCAACTTTAGCTGTGTTTAGATAGTTCAGCAGTTGACTTCCCGATATATATAAATTTCAAACCTCCCCCATCCTCAAGAAAAAGAGAGCTTGTTTTCAAATTTACTTGCGTAGAATTATGGGTTGAAGACAACATTTGTCAAGCAGTTGAGATAATTTGTGTACAGAGGGCTGTTGGTTTGAGAACTATGGCGATAAAAATAACGTTGAAATGTTTTAGATACAAATGGATAGCTTACATGTTATTGAGCTCCCCTCTTTGCTTCACACATGTTAAAGTTCATATTGTCTATGTGCTTTTGGCTGTGCATTTTTATCAAACCTCCTGTCGTAATGCAGATTTCCAATTCTGTTGCAGTGCACGATTGCCAATTGGTTGACGATATTCCAGTTGAGAAGCTATTAGTCCATGACGTGCCTGTAGACATCGTATGCACTCCGACGCAGGTCATTTTAACCAACACGAGAATCCCCAAACCCCAAGGTTAGCAGCTTATTTGTAGACTTTTTAAGCAAAATTAATCATAGTAAAGCTGCCTTTTCTTTTTATTGCATATCTCAAAAGATTAGTGTAGCACATTTGAATTTGCTTAGATTGAAGGGACATGAGTTTATGAACAAATTTGTAATATCAACTGATAGTGTCTCATATATACAACCCAAAATGTTTTGAATTGCAGGCATTTACTGGGAAATGTTGTCTCCCGAGAAGCTGAGCCAAGTTCGAATACTCAGGGAGCTCAAACGTCGTATCGAACGGGAGACTGGAAAACCACTGCCATGTGGTCCGTCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGGAAAACGTGCATCCCCTAAGAAGTGATGAAGGGGAAAATGGAGAAAGAAACAAACCTAAATTAGTTTTTTTCCCCCCCTTTTTCCTTCATTATAAAGTTTCATTGTTGAATAAACACTGTCCTAAAACAGACGAGGAAGATTTGAAAGTAACAAAGAAATGAAAATATAAGAGGGAGTGTTTTCTTCCCTAA

mRNA sequence

AGAATATTAAAAAAATAAAAAATAAAAATCTTATCTTCATCCGTGGCGAACTATCCGCGAGGCGCGAACACTCGAACAGTACACTGAAATTCTCGTTAATGGATTCCAATCTCCTGCAATTATCCCACACACTATCACTCAAATCCACATTCCTCAACGCAATTCGAAGATCCCCTTCCATCTCTTCAATCTCCATCAGAAGAAATTTTCAGTTCCAGAGGCACTTCAAGCTGGAGAGCAGTAAAAATGGTGGCAGAGAAACCCAACGGGATTGTGCTTTCGACGAAGTAGCATTCGAGGCCGAGAGATCTCGCCTCGACGCCCAAGCTGTAAAATCCATGGCCGAAGCTTCAATTAGGGACACTGAAGGCGCTTCCGACGACGACCCGAAAGCTTGGAAGTGGATCATCCGAAAACGAGTTTGGGATTTCATGGAGTCACAAAATATCGCGGCAAATCCCCGTCCCGTTCACCATCGAATCCCTAATTTCCTTGGCGCCATGGAAGCTGCCAATAGATTGTGCGATTTGGAAGTATTCAGGGATTCACAGTGCGTGAAGGTTAATCCGGATTCTCCTCAGAAGGGTGTGAGGCTTCTTACACTTACGGGTGGCAAAAAACTGTTAACACCTCAGCCTCGGTTAAGAACAGGGTTTTTCTCCATAGTCGAATCAGGGATGTTGACTCCGGCTACCATCAACGAGGCCTGCACTTCTGTTGGGGTAGCAAAGTATGGAAAGCCTATTGGACTGGATGAAAAGATCAAAGTTGATCTGATCATCATTGGTTCTGTTGCTGTTGACCCCAGAACGGGTGCTCGGCTTGGCAAGGGAGAGATTTCCAATTCTGTTGCAGTGCACGATTGCCAATTGGTTGACGATATTCCAGTTGAGAAGCTATTAGTCCATGACGTGCCTGTAGACATCGTATGCACTCCGACGCAGGTCATTTTAACCAACACGAGAATCCCCAAACCCCAAGGCATTTACTGGGAAATGTTGTCTCCCGAGAAGCTGAGCCAAGTTCGAATACTCAGGGAGCTCAAACGTCGTATCGAACGGGAGACTGGAAAACCACTGCCATGTGGTCCGTCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGGAAAACGTGCATCCCCTAAGAAGTGATGAAGGGGAAAATGGAGAAAGAAACAAACCTAAATTAGTTTTTTTCCCCCCCTTTTTCCTTCATTATAAAGTTTCATTGTTGAATAAACACTGTCCTAAAACAGACGAGGAAGATTTGAAAGTAACAAAGAAATGAAAATATAAGAGGGAGTGTTTTCTTCCCTAA

Coding sequence (CDS)

ATGGATTCCAATCTCCTGCAATTATCCCACACACTATCACTCAAATCCACATTCCTCAACGCAATTCGAAGATCCCCTTCCATCTCTTCAATCTCCATCAGAAGAAATTTTCAGTTCCAGAGGCACTTCAAGCTGGAGAGCAGTAAAAATGGTGGCAGAGAAACCCAACGGGATTGTGCTTTCGACGAAGTAGCATTCGAGGCCGAGAGATCTCGCCTCGACGCCCAAGCTGTAAAATCCATGGCCGAAGCTTCAATTAGGGACACTGAAGGCGCTTCCGACGACGACCCGAAAGCTTGGAAGTGGATCATCCGAAAACGAGTTTGGGATTTCATGGAGTCACAAAATATCGCGGCAAATCCCCGTCCCGTTCACCATCGAATCCCTAATTTCCTTGGCGCCATGGAAGCTGCCAATAGATTGTGCGATTTGGAAGTATTCAGGGATTCACAGTGCGTGAAGGTTAATCCGGATTCTCCTCAGAAGGGTGTGAGGCTTCTTACACTTACGGGTGGCAAAAAACTGTTAACACCTCAGCCTCGGTTAAGAACAGGGTTTTTCTCCATAGTCGAATCAGGGATGTTGACTCCGGCTACCATCAACGAGGCCTGCACTTCTGTTGGGGTAGCAAAGTATGGAAAGCCTATTGGACTGGATGAAAAGATCAAAGTTGATCTGATCATCATTGGTTCTGTTGCTGTTGACCCCAGAACGGGTGCTCGGCTTGGCAAGGGAGAGATTTCCAATTCTGTTGCAGTGCACGATTGCCAATTGGTTGACGATATTCCAGTTGAGAAGCTATTAGTCCATGACGTGCCTGTAGACATCGTATGCACTCCGACGCAGGTCATTTTAACCAACACGAGAATCCCCAAACCCCAAGGCATTTACTGGGAAATGTTGTCTCCCGAGAAGCTGAGCCAAGTTCGAATACTCAGGGAGCTCAAACGTCGTATCGAACGGGAGACTGGAAAACCACTGCCATGTGGTCCGTCGGAGAAACTACCACCTACAGCTCAACGAAGCTCGAAACCTGGAAAACGTGCATCCCCTAAGAAGTGA

Protein sequence

MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGARLGKGEISNSVAVHDCQLVDDIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQRSSKPGKRASPKK

Homology

[Close]

BLAST of Lsi08G005630 vs. ExPASy Swiss-Prot
Match: Q9SRE0 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Arabidopsis thaliana OX=3702 GN=COG0212 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.0e-112
Identity = 203/312 (65.06%), Postives = 243/312 (77.88%), Query Frame = 0


Query: 52  GRETQRDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDF 111
           G E     AFD VA+EA+R  LDA A++ MAE + ++ E   D DPKAWKW+IRK++WD 
Sbjct: 43  GSENGDGVAFDAVAYEADRLSLDAAAMEDMAETAKKELESDPDSDPKAWKWVIRKKMWDL 102

Query: 112 MESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTG 171
           ME++N A +PRPVHHRIPNF+GA  AA +L +L+ FR +  VKVNPDSPQK +R LTL+G
Sbjct: 103 MEARNYAMSPRPVHHRIPNFVGASAAARKLAELDAFRMAMVVKVNPDSPQKQIRFLTLSG 162

Query: 172 GKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGS 231
            KKLLTPQPRLRTGFFS++ES +L P TI EACTSVGVAKYG+ IGLDEKIKVDLI+IGS
Sbjct: 163 EKKLLTPQPRLRTGFFSVLESDLLKPETIMEACTSVGVAKYGRAIGLDEKIKVDLIVIGS 222

Query: 232 VAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDV 291
           VAV+P+TGARLGKGE               I +S      VHDCQLVDDIP+EKL +HDV
Sbjct: 223 VAVNPQTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTTVHDCQLVDDIPLEKLAIHDV 282

Query: 292 PVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPS 345
           PVDI+CTPT+VI TNT IPKPQGIYW+ LSPEKL Q+RILRELK R+E++TG+ LP GPS
Sbjct: 283 PVDIICTPTRVIFTNTPIPKPQGIYWDKLSPEKLRQIRILRELKNRLEKKTGRKLPTGPS 342

[Close]

BLAST of Lsi08G005630 vs. ExPASy Swiss-Prot
Match: Q0P464 (Methenyltetrahydrofolate synthase domain-containing protein OS=Danio rerio OX=7955 GN=mthfsd PE=2 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 6.7e-52
Identity = 111/245 (45.31%), Postives = 156/245 (63.67%), Query Frame = 0


Query: 101 KWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSP 160
           KW +R +VW+++E +N+A  PRPVH+RIPNF GA+EA N++  LE+F +S  VKV+PD P
Sbjct: 13  KWDVRHKVWNYIEVKNLANFPRPVHNRIPNFKGALEACNKVAQLEIFIESAVVKVDPDKP 72

Query: 161 QKGVRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLD 220
            +GVRL  L   K LL P PRLR G F+ I      T  T+    TS G+ ++  P+GLD
Sbjct: 73  MEGVRLAALKARKSLLVPTPRLRFGLFNRITPPKGATKETLRVCSTSQGIKEFSVPVGLD 132

Query: 221 EKIKVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVD 280
           +K++VDL+++GSVAV  + G R+GKGE               ++ S      VHDCQ++ 
Sbjct: 133 DKVQVDLVVVGSVAVSEK-GYRIGKGEGFADMEYAMMACMGSVTESTWVITVVHDCQVM- 192

Query: 281 DIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIE 326
           DIP E +  HD+ VD + T T+VI T  + PKPQGI W ML  E+L ++ IL++L R +E
Sbjct: 193 DIPEELIERHDLMVDFIITATRVIKTECKHPKPQGIIWSMLHKEELKKIPILKKL-RTLE 252

[Close]

BLAST of Lsi08G005630 vs. ExPASy Swiss-Prot
Match: Q2KI24 (Methenyltetrahydrofolate synthase domain-containing protein OS=Bos taurus OX=9913 GN=MTHFSD PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.8e-50
Identity = 116/261 (44.44%), Postives = 151/261 (57.85%), Query Frame = 0


Query: 104 IRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKG 163
           IR+++WD+MESQN+A  PRPVHHRIPNF GA  AA     L+ F+ ++ +KVNPD+PQK 
Sbjct: 12  IREQIWDYMESQNLADFPRPVHHRIPNFKGASRAAEHFPRLQAFKMARTIKVNPDAPQKN 71

Query: 164 VRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLDEKI 223
            R   L   K LL P PRLRTG F+ I      T   + +  TS GV  Y  P+GLD K+
Sbjct: 72  ARFFVLESKKTLLVPTPRLRTGLFNKITPPPGATKDILRKCATSQGVRNYSTPVGLDSKV 131

Query: 224 KVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIP 283
            VDL+++GSVAV  + G R+GKGE               +S        VHDCQ+V DIP
Sbjct: 132 LVDLVVVGSVAVSEK-GWRIGKGEGYADLEYAMMVSMGAVSQGTPVVTIVHDCQVV-DIP 191

Query: 284 VEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERET 343
              L  HD+ VD + TPT+VI T    PKP GI W  +S E L ++ ILR L+ + E + 
Sbjct: 192 EALLEDHDLTVDYILTPTRVITTGCERPKPAGIAWSKISCEMLGKMPILRSLRHQ-EEQA 251

Query: 344 GKPLPCGPSEKLPPTAQRSSK 345
           GK +      + PP A RS +
Sbjct: 252 GKDVTLRDGPRSPPGATRSPR 269

[Close]

BLAST of Lsi08G005630 vs. ExPASy Swiss-Prot
Match: Q52L34 (Methenyltetrahydrofolate synthase domain-containing protein OS=Xenopus laevis OX=8355 GN=mthfsd PE=2 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 8.2e-50
Identity = 120/276 (43.48%), Postives = 160/276 (57.97%), Query Frame = 0


Query: 96  DPKAWKWIIRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKV 155
           DP   KW IR++VWD++E+ N+A  PRPVHHRIPNF  + +A   + DLEVFR +  VKV
Sbjct: 8   DPGCSKWDIRQKVWDYIENNNLADFPRPVHHRIPNFKESHQACQNIIDLEVFRRTWEVKV 67

Query: 156 NPDSPQKGVRLLTLTGGKKLLTPQPRLRTGFFSIVESGMLTPATINEAC-TSVGVAKYGK 215
           +PD P +GVRL  L   K LL P PRLRTG F+ +         +   C TS GV  Y  
Sbjct: 68  DPDKPLEGVRLAALQARKTLLVPTPRLRTGLFNKITPPPGANKEVLRICSTSQGVKDYSV 127

Query: 216 PIGLDEKIKVDLIIIGSVAVDPRTGARLGKGE----------ISNS---------VAVHD 275
           P+GLD K++VDL+++GSVAV  + G R+GKGE          +S             VHD
Sbjct: 128 PMGLDAKVQVDLVVVGSVAVSVQ-GWRIGKGEGFADMEYAMMVSMGAVTEHTVVVTVVHD 187

Query: 276 CQLVDDIPVEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILREL 335
           CQ+V DIP E L  HD+ VD + TPT++I T+ +  KPQGI W M++ E + ++ ILR L
Sbjct: 188 CQVV-DIPEELLEDHDLTVDFILTPTRIIKTDCKRAKPQGIIWSMITREMMGKIPILRNL 247

Query: 336 KRRIERETGKPLPCGPSEKLPPTAQRSSKPGKRASP 352
           + R ER  GK +              +S+ G R SP
Sbjct: 248 QDR-ERRAGKDVSLKDG--------NASEQGSRQSP 272

[Close]

BLAST of Lsi08G005630 vs. ExPASy Swiss-Prot
Match: Q2M296 (Methenyltetrahydrofolate synthase domain-containing protein OS=Homo sapiens OX=9606 GN=MTHFSD PE=1 SV=2)

HSP 1 Score: 196.1 bits (497), Expect = 6.9e-49
Identity = 117/263 (44.49%), Postives = 156/263 (59.32%), Query Frame = 0


Query: 104 IRKRVWDFMESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKG 163
           IR+++W +MESQN+A  PRPVHHRIPNF G+  A   + DL+VF  +Q VKV+PD P +G
Sbjct: 13  IREQIWGYMESQNLADFPRPVHHRIPNFKGSYLACQNIKDLDVFARTQEVKVDPDKPLEG 72

Query: 164 VRLLTLTGGKKLLTPQPRLRTGFFS-IVESGMLTPATINEACTSVGVAKYGKPIGLDEKI 223
           VRLL L   K LL P PRLRTG F+ I      T   + +  TS GV  Y  PIGLD ++
Sbjct: 73  VRLLVLQSKKTLLVPTPRLRTGLFNKITPPPGATKDILRKCATSQGVRNYSVPIGLDSRV 132

Query: 224 KVDLIIIGSVAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIP 283
            VDL+++GSVAV  + G R+GKGE               +S        VHDCQ+V DIP
Sbjct: 133 LVDLVVVGSVAVSEK-GWRIGKGEGYADLEYAMMVSMGAVSKETPVVTIVHDCQVV-DIP 192

Query: 284 VEKLLVHDVPVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERET 343
            E +  HD+ VD + TPT+VI T  + PKP GI W  +S E + ++ ILR L+ R E++ 
Sbjct: 193 EELVEEHDITVDYILTPTRVIATGCKRPKPMGITWFKISLEMMEKIPILRSLRAR-EQQA 252

Query: 344 GKPLPC-GPSEKLPPTAQRSSKP 346
           GK +   G  + LP    + + P
Sbjct: 253 GKDVTLQGEHQHLPEPGCQQTVP 272

[Close]

BLAST of Lsi08G005630 vs. ExPASy TrEMBL
Match: A0A6J1FMW8 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445648 PE=4 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.1e-173
Identity = 318/371 (85.71%), Postives = 335/371 (90.30%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI + RNFQFQRHFKLESSKNGGR+TQRD A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTEGA DDDPKAWKW+IRKR+WDFMESQNIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGALDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGE               I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYW+MLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWDMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. ExPASy TrEMBL
Match: A0A6J1FIK2 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445648 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 1.0e-172
Identity = 317/371 (85.44%), Postives = 334/371 (90.03%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI + RNFQFQRHFKLESSKNGGR+TQRD A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTEGA DDDPKAWKW+IRKR+WDFMESQNIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGALDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGK---------------GEISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGK               G I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKVQGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYW+MLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWDMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. ExPASy TrEMBL
Match: A0A6J1K2I1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489504 PE=4 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.8e-172
Identity = 317/371 (85.44%), Postives = 335/371 (90.30%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI I RNFQFQRHFKLESSKNGGR+TQR+ A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPIGRNFQFQRHFKLESSKNGGRKTQREGA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTEGASDDDPKAWKW+IRKR+WDFMES NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGASDDDPKAWKWVIRKRIWDFMESLNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYG+PIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGEPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGE               I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYWEMLSPEKLSQVR+LRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRVLRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. ExPASy TrEMBL
Match: A0A6J1K0E7 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489504 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 8.7e-172
Identity = 316/371 (85.18%), Postives = 334/371 (90.03%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI I RNFQFQRHFKLESSKNGGR+TQR+ A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPIGRNFQFQRHFKLESSKNGGRKTQREGA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTEGASDDDPKAWKW+IRKR+WDFMES NIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGASDDDPKAWKWVIRKRIWDFMESLNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYG+PIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGEPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGK---------------GEISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGK               G I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKVQGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYWEMLSPEKLSQVR+LRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRVLRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. ExPASy TrEMBL
Match: A0A1S3B497 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485607 PE=4 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 2.2e-167
Identity = 310/374 (82.89%), Postives = 331/374 (88.50%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQF--QRHFKLESSKNGGRETQRD 60
           MDS L+QLS  LSLKST LNAIR  PSISSIS+R +     +RHFKLES+KNGG +TQRD
Sbjct: 1   MDSILMQLSQPLSLKSTLLNAIRTPPSISSISLRGSLHLIQRRHFKLESTKNGGTDTQRD 60

Query: 61  CAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIA 120
             FDE AFEAERSRLDAQA KSMAEAS+RDTEGASDDDPKAWKW+IRKR+WDFMESQN+A
Sbjct: 61  LPFDEAAFEAERSRLDAQARKSMAEASMRDTEGASDDDPKAWKWVIRKRIWDFMESQNVA 120

Query: 121 ANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTP 180
           ANPRPVHHRIPNF+GAMEAANRLC+LEVF DSQCVKVNPDSPQKGVRLLTLTGGKKLLTP
Sbjct: 121 ANPRPVHHRIPNFIGAMEAANRLCELEVFSDSQCVKVNPDSPQKGVRLLTLTGGKKLLTP 180

Query: 181 QPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRT 240
           QPRLRTGFFSIVESGMLTPATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDP+T
Sbjct: 181 QPRLRTGFFSIVESGMLTPATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPKT 240

Query: 241 GARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCT 300
           GARLGKGE               I +S     +VHDCQLVDDIPV+KLLVHDVPVDIVCT
Sbjct: 241 GARLGKGEGFAELEYGMLRYMGAIDDSTLIVTSVHDCQLVDDIPVQKLLVHDVPVDIVCT 300

Query: 301 PTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTA 354
           PTQVILTNT+IPKPQGIYWEMLSPEKLSQ+RILRELKRRIERETGK LPCGPSEKLPPTA
Sbjct: 301 PTQVILTNTKIPKPQGIYWEMLSPEKLSQIRILRELKRRIERETGKLLPCGPSEKLPPTA 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: KAG7016410.1 (5-formyltetrahydrofolate cyclo-ligase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 619.4 bits (1596), Expect = 1.9e-173
Identity = 319/371 (85.98%), Postives = 335/371 (90.30%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI + RNFQFQRHFKLESSKNGGR+TQRD A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTE ASDDDPKAWKW+IRKR+WDFMESQNIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTESASDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGE               I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRHMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: KAG6578879.1 (5-formyltetrahydrofolate cyclo-ligase-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 619.4 bits (1596), Expect = 1.9e-173
Identity = 319/371 (85.98%), Postives = 335/371 (90.30%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI + RNFQFQRHFKLESSKNGGR+TQRD A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTE ASDDDPKAWKW+IRKR+WDFMESQNIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTESASDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGE               I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: XP_038884048.1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 [Benincasa hispida])

HSP 1 Score: 619.4 bits (1596), Expect = 1.9e-173
Identity = 323/372 (86.83%), Postives = 334/372 (89.78%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS LLQLSH LSLKSTFLNAI RSPSISSISI       RHFKLES+KNGGRETQRD  
Sbjct: 1   MDSRLLQLSHPLSLKSTFLNAIPRSPSISSISI-------RHFKLESTKNGGRETQRDVP 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE A+EAERSRLDAQA+KSMAEASIRDTEGASDDDPKAWKW+IRKR+WDFMESQNIAAN
Sbjct: 61  FDEAAYEAERSRLDAQAIKSMAEASIRDTEGASDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNFLGAMEAANRLCDL VFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFLGAMEAANRLCDLVVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLTPATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDP+TGA
Sbjct: 181 RLRTGFFSIVESGMLTPATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPKTGA 240

Query: 241 RLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGE               I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 354
           QVILTNT+IPKPQGIYWEMLSPEKLSQVRILRELKRRIERETG+PLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGRPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: XP_022939928.1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita moschata])

HSP 1 Score: 618.2 bits (1593), Expect = 4.3e-173
Identity = 318/371 (85.71%), Postives = 335/371 (90.30%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI + RNFQFQRHFKLESSKNGGR+TQRD A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPVGRNFQFQRHFKLESSKNGGRKTQRDSA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTEGA DDDPKAWKW+IRKR+WDFMESQNIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGALDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGE               I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYW+MLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWDMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. NCBI nr
Match: XP_023551592.1 (5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.2 bits (1593), Expect = 4.3e-173
Identity = 319/371 (85.98%), Postives = 336/371 (90.57%), Query Frame = 0


Query: 1   MDSNLLQLSHTLSLKSTFLNAIRRSPSISSISIRRNFQFQRHFKLESSKNGGRETQRDCA 60
           MDS+ LQLS  LS+KSTFLNAIR++ SI SI I RNFQF+RHFKLESSKNGGR+TQR+ A
Sbjct: 1   MDSSFLQLSRPLSVKSTFLNAIRKTSSIYSIPIGRNFQFRRHFKLESSKNGGRKTQREGA 60

Query: 61  FDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDFMESQNIAAN 120
           FDE AFEAERSRLDA A+KSMAEAS+RDTEGASDDDPKAWKW+IRKR+WDFMESQNIAAN
Sbjct: 61  FDEAAFEAERSRLDALAMKSMAEASVRDTEGASDDDPKAWKWVIRKRIWDFMESQNIAAN 120

Query: 121 PRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTGGKKLLTPQP 180
           PRPVHHRIPNF+GAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTL GGKKLLTPQP
Sbjct: 121 PRPVHHRIPNFVGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLMGGKKLLTPQP 180

Query: 181 RLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGSVAVDPRTGA 240
           RLRTGFFSIVESGMLT ATI EACTSVGVAKYGKPIGLDEKIKVDLI+IGSVAVDPRTGA
Sbjct: 181 RLRTGFFSIVESGMLTSATIKEACTSVGVAKYGKPIGLDEKIKVDLIVIGSVAVDPRTGA 240

Query: 241 RLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300
           RLGKGE               I +S     +VHDCQLVDDIPVEKLLVHDVPVDIVCTPT
Sbjct: 241 RLGKGEGFAELEYGMLRYMGAIDDSTPIVTSVHDCQLVDDIPVEKLLVHDVPVDIVCTPT 300

Query: 301 QVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 353
           QVILTNT+IPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR
Sbjct: 301 QVILTNTKIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPSEKLPPTAQR 360

[Close]

BLAST of Lsi08G005630 vs. TAIR 10
Match: AT1G76730.1 (NagB/RpiA/CoA transferase-like superfamily protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.4e-113
Identity = 203/312 (65.06%), Postives = 243/312 (77.88%), Query Frame = 0


Query: 52  GRETQRDCAFDEVAFEAERSRLDAQAVKSMAEASIRDTEGASDDDPKAWKWIIRKRVWDF 111
           G E     AFD VA+EA+R  LDA A++ MAE + ++ E   D DPKAWKW+IRK++WD 
Sbjct: 43  GSENGDGVAFDAVAYEADRLSLDAAAMEDMAETAKKELESDPDSDPKAWKWVIRKKMWDL 102

Query: 112 MESQNIAANPRPVHHRIPNFLGAMEAANRLCDLEVFRDSQCVKVNPDSPQKGVRLLTLTG 171
           ME++N A +PRPVHHRIPNF+GA  AA +L +L+ FR +  VKVNPDSPQK +R LTL+G
Sbjct: 103 MEARNYAMSPRPVHHRIPNFVGASAAARKLAELDAFRMAMVVKVNPDSPQKQIRFLTLSG 162

Query: 172 GKKLLTPQPRLRTGFFSIVESGMLTPATINEACTSVGVAKYGKPIGLDEKIKVDLIIIGS 231
            KKLLTPQPRLRTGFFS++ES +L P TI EACTSVGVAKYG+ IGLDEKIKVDLI+IGS
Sbjct: 163 EKKLLTPQPRLRTGFFSVLESDLLKPETIMEACTSVGVAKYGRAIGLDEKIKVDLIVIGS 222

Query: 232 VAVDPRTGARLGKGE---------------ISNS----VAVHDCQLVDDIPVEKLLVHDV 291
           VAV+P+TGARLGKGE               I +S      VHDCQLVDDIP+EKL +HDV
Sbjct: 223 VAVNPQTGARLGKGEGFAELEYGMLRYMGAIDDSTPVVTTVHDCQLVDDIPLEKLAIHDV 282

Query: 292 PVDIVCTPTQVILTNTRIPKPQGIYWEMLSPEKLSQVRILRELKRRIERETGKPLPCGPS 345
           PVDI+CTPT+VI TNT IPKPQGIYW+ LSPEKL Q+RILRELK R+E++TG+ LP GPS
Sbjct: 283 PVDIICTPTRVIFTNTPIPKPQGIYWDKLSPEKLRQIRILRELKNRLEKKTGRKLPTGPS 342

The following BLAST results are available for this feature:

BLAST of Lsi08G005630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRE0	2.0e-112	65.06	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 OS=Arabidopsis thalia...	[more]
Q0P464	6.7e-52	45.31	Methenyltetrahydrofolate synthase domain-containing protein OS=Danio rerio OX=79...	[more]
Q2KI24	2.8e-50	44.44	Methenyltetrahydrofolate synthase domain-containing protein OS=Bos taurus OX=991...	[more]
Q52L34	8.2e-50	43.48	Methenyltetrahydrofolate synthase domain-containing protein OS=Xenopus laevis OX...	[more]
Q2M296	6.9e-49	44.49	Methenyltetrahydrofolate synthase domain-containing protein OS=Homo sapiens OX=9...	[more]

BLAST of Lsi08G005630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FMW8	2.1e-173	85.71	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbi...	[more]
A0A6J1FIK2	1.0e-172	85.44	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbi...	[more]
A0A6J1K2I1	1.8e-172	85.44	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 OS=Cucurbi...	[more]
A0A6J1K0E7	8.7e-172	85.18	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucurbi...	[more]
A0A1S3B497	2.2e-167	82.89	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X2 OS=Cucumis...	[more]

BLAST of Lsi08G005630 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016410.1	1.9e-173	85.98	5-formyltetrahydrofolate cyclo-ligase-like protein [Cucurbita argyrosperma subsp...	[more]
KAG6578879.1	1.9e-173	85.98	5-formyltetrahydrofolate cyclo-ligase-like protein, partial [Cucurbita argyrospe...	[more]
XP_038884048.1	1.9e-173	86.83	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 [Benincasa hispida]	[more]
XP_022939928.1	4.3e-173	85.71	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita...	[more]
XP_023551592.1	4.3e-173	85.98	5-formyltetrahydrofolate cyclo-ligase-like protein COG0212 isoform X1 [Cucurbita...	[more]

BLAST of Lsi08G005630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G76730.1	1.4e-113	65.06	NagB/RpiA/CoA transferase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024185	5-formyltetrahydrofolate cyclo-ligase-like domain superfamily	GENE3D	3.40.50.10420		coord: 133..289 e-value: 3.4E-9 score: 38.4
IPR002698	5-formyltetrahydrofolate cyclo-ligase	PFAM	PF01812	5-FTHF_cyc-lig	coord: 101..246 e-value: 8.0E-23 score: 81.3
IPR002698	5-formyltetrahydrofolate cyclo-ligase	PANTHER	PTHR13017	5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE-RELATED	coord: 88..247 coord: 252..347
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 322..353
IPR037171	NagB/RpiA transferase-like	SUPERFAMILY	100950	NagB/RpiA/CoA transferase-like	coord: 100..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G005630.1	Lsi08G005630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity