Lsi08G005310 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G005310
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionVQ domain-containing protein
Locationchr08: 13276564 .. 13277076 (+)
RNA-Seq ExpressionLsi08G005310
SyntenyLsi08G005310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTGAAAGAAAAGACGTTCAACTCCAAGGCCCCCGCCCCGCCGCCCTCAAGATCACCAAAGACTCCCACAAGATCAAAAAGCCGCCGCTCCCACCACCGCACCGGCCCGTCATCATTTACACCGTCTCTCCCAAGATCATCCACACTGAACCTACCGAGTTCAAGGACTTGGTCCAACGCCTCACCGGCTGCCGACCCTCGTCCTCCAATGATGCCCTTGATCATCCCTCGGATACAACTATATCATCGATGTCGATGTCGATGCCGATGAGTACAGATCAAGGAGTGGAGAGGGTAGGTCATGGGATATTATCGCCAACGCCGGGGTTGCTGCCACCGATTTCGCCGAATATTTTCACTCCACCGCCTCTGCCGCCACAGACCACCATGCCGGCGGTCGCTGATCTCAGCCCTTTTACACACTTTTTCCAAGACCTTAGTCCTATAGCCACCAATCATTTTTCCTCATCTCTAGATTTCTTCCAACAAAACTTCCCTGATTTCCATTAA

mRNA sequence

ATGCCTGAAAGAAAAGACGTTCAACTCCAAGGCCCCCGCCCCGCCGCCCTCAAGATCACCAAAGACTCCCACAAGATCAAAAAGCCGCCGCTCCCACCACCGCACCGGCCCGTCATCATTTACACCGTCTCTCCCAAGATCATCCACACTGAACCTACCGAGTTCAAGGACTTGGTCCAACGCCTCACCGGCTGCCGACCCTCGTCCTCCAATGATGCCCTTGATCATCCCTCGGATACAACTATATCATCGATGTCGATGTCGATGCCGATGAGTACAGATCAAGGAGTGGAGAGGGTAGGTCATGGGATATTATCGCCAACGCCGGGGTTGCTGCCACCGATTTCGCCGAATATTTTCACTCCACCGCCTCTGCCGCCACAGACCACCATGCCGGCGGTCGCTGATCTCAGCCCTTTTACACACTTTTTCCAAGACCTTAGTCCTATAGCCACCAATCATTTTTCCTCATCTCTAGATTTCTTCCAACAAAACTTCCCTGATTTCCATTAA

Coding sequence (CDS)

ATGCCTGAAAGAAAAGACGTTCAACTCCAAGGCCCCCGCCCCGCCGCCCTCAAGATCACCAAAGACTCCCACAAGATCAAAAAGCCGCCGCTCCCACCACCGCACCGGCCCGTCATCATTTACACCGTCTCTCCCAAGATCATCCACACTGAACCTACCGAGTTCAAGGACTTGGTCCAACGCCTCACCGGCTGCCGACCCTCGTCCTCCAATGATGCCCTTGATCATCCCTCGGATACAACTATATCATCGATGTCGATGTCGATGCCGATGAGTACAGATCAAGGAGTGGAGAGGGTAGGTCATGGGATATTATCGCCAACGCCGGGGTTGCTGCCACCGATTTCGCCGAATATTTTCACTCCACCGCCTCTGCCGCCACAGACCACCATGCCGGCGGTCGCTGATCTCAGCCCTTTTACACACTTTTTCCAAGACCTTAGTCCTATAGCCACCAATCATTTTTCCTCATCTCTAGATTTCTTCCAACAAAACTTCCCTGATTTCCATTAA

Protein sequence

MPERKDVQLQGPRPAALKITKDSHKIKKPPLPPPHRPVIIYTVSPKIIHTEPTEFKDLVQRLTGCRPSSSNDALDHPSDTTISSMSMSMPMSTDQGVERVGHGILSPTPGLLPPISPNIFTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH
Homology
BLAST of Lsi08G005310 vs. ExPASy Swiss-Prot
Match: Q8LGD5 (Protein MKS1 OS=Arabidopsis thaliana OX=3702 GN=MKS1 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 2.9e-13
Identity = 64/181 (35.36%), Postives = 85/181 (46.96%), Query Frame = 0

Query: 3   ERKDVQLQGPRPAALKITKDSHKIKKP---PLPPPHR----------PVIIYTVSPKIIH 62
           +++ +Q+ GPRP+ L + KDSHKIKKP   P PPP+R          PV+IY VSPK++H
Sbjct: 18  QKRQLQICGPRPSPLSVHKDSHKIKKPPKHPAPPPNRDQPPPYIPREPVVIYAVSPKVVH 77

Query: 63  TEPTEFKDLVQRLTGCRP---------SSSNDALDHPSDTTISSMSMSMPMSTDQGVE-- 122
              +EF ++VQRLTG               + A    S    S      P + D+ VE  
Sbjct: 78  ATASEFMNVVQRLTGISSGVFLESGGGGDVSPAARLASTENASPRGGKEPAARDETVEIN 137

Query: 123 ----------RVGHGILSPTPGLLPPISPNIFTP---------PPLPPQTTMPAVADLSP 141
                         GILSP+P LLP  S  IF+P         P +P     PA   +SP
Sbjct: 138 TAMEEAAEFGGYAPGILSPSPALLPTASTGIFSPMYHQGGMFSPAIPLGLFSPA-GFMSP 197

BLAST of Lsi08G005310 vs. ExPASy Swiss-Prot
Match: F4HWF9 (Nuclear speckle RNA-binding protein B OS=Arabidopsis thaliana OX=3702 GN=NSRB PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 7.2e-12
Identity = 50/118 (42.37%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 11  GPRPAALKITKDSHK-IKKPPLPPPH--------------------RPVIIYTVSPKIIH 70
           GP+P  LK+  DSHK IKKPPL PPH                     PV IYTV+P+IIH
Sbjct: 13  GPKPTPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPSQSRPPPGPVNIYTVTPRIIH 72

Query: 71  TEPTEFKDLVQRLTGCRPSSSNDALDHPSDTTISSMSMSMPMSTDQGVERVGHGILSP 108
           T P  F  LVQRLTG   +S+         ++ SS S S P  T   V+   HG++SP
Sbjct: 73  THPNNFMTLVQRLTGQTSTSTT--------SSSSSSSTSEPKDTSTMVD-TSHGLISP 121

BLAST of Lsi08G005310 vs. ExPASy Swiss-Prot
Match: Q9LS54 (VQ motif-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=VQ20 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 8.9e-10
Identity = 35/67 (52.24%), Postives = 41/67 (61.19%), Query Frame = 0

Query: 12  PRPAALKITKDSHKIKKPPLP-------PPHRPVIIYTVSPKIIHTEPTEFKDLVQRLTG 71
           P P  LK+ KDSH IKKPP P        P  PVIIYT +P+IIHT P +F  LVQ+LTG
Sbjct: 41  PSPPTLKVNKDSHVIKKPPSPSSFSSAAKPRHPVIIYTHTPRIIHTNPKDFMALVQKLTG 100

BLAST of Lsi08G005310 vs. ExPASy Swiss-Prot
Match: Q9CA36 (VQ motif-containing protein 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VQ8 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.3e-05
Identity = 45/128 (35.16%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 8   QLQGPRPAALKITKDSHKIKK----PPLPPPH---RPVIIYTVSPKIIHTEPTEFKDLVQ 67
           ++ G RP++LK+  +SH IKK       P PH    PVIIY  SPK+IHT   +F  LVQ
Sbjct: 8   EINGSRPSSLKLAGESHTIKKTSSCKSKPRPHGRASPVIIYAHSPKVIHTRAEDFMALVQ 67

Query: 68  RLTGC-----RPSSSNDALDHPSDTTISSMSMSMPMSTDQGVERVGHGILSPTPGLLPPI 124
           RLTG      R +S + +     +  +   + + P S D    R     L+  P L  P 
Sbjct: 68  RLTGLDEIIRRNTSESSSSVVTEEVNVGDDNTAAPFSQD----RTQRQKLTDMP-LFTPS 127

BLAST of Lsi08G005310 vs. ExPASy TrEMBL
Match: A0A0A0KI80 (VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G338080 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.4e-53
Identity = 124/179 (69.27%), Postives = 134/179 (74.86%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPP--PHRPVIIYTVSPKIIHTEPTEFKDL 60
           MPE+K++QLQGPRP+ALKITKDSHKIKKPPLPP  PHRPVIIYTVSPKIIHT+PTEFKDL
Sbjct: 1   MPEKKELQLQGPRPSALKITKDSHKIKKPPLPPPQPHRPVIIYTVSPKIIHTDPTEFKDL 60

Query: 61  VQRLTGCR---PSSSNDAL---DHPSDTTISSMSMSMPMSTDQGVER-VGHGILSPTPGL 120
           VQRLTG      SSSN AL    HP    IS  +   P+    GV+R + HGILSPTPGL
Sbjct: 61  VQRLTGHHQPPSSSSNHALVHDHHPPPDHISIPTTDDPIDDQAGVDRIIAHGILSPTPGL 120

Query: 121 LPPISPNIFTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           LPPIS  IF PPP  P     A  DL+  T FF DLSPIA NHFSSSLDFFQQNFPDFH
Sbjct: 121 LPPISSTIFNPPPQAPAAA--AAGDLTSLTQFFHDLSPIAANHFSSSLDFFQQNFPDFH 177

BLAST of Lsi08G005310 vs. ExPASy TrEMBL
Match: A0A5A7UZA1 (Protein MKS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold98G00830 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 5.7e-52
Identity = 122/178 (68.54%), Postives = 132/178 (74.16%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPP--PHRPVIIYTVSPKIIHTEPTEFKDL 60
           MPE+K++QLQGPRP+ALKITKDSHKIKKPPLPP  PHRPVIIYTVSPKIIHT+PT+FKDL
Sbjct: 1   MPEKKELQLQGPRPSALKITKDSHKIKKPPLPPPQPHRPVIIYTVSPKIIHTDPTQFKDL 60

Query: 61  VQRLTGCR---PSSSNDAL--DHPSDTTISSMSMSMPMSTDQGVER-VGHGILSPTPGLL 120
           VQRLTG      SSSN AL  DHP    IS            GV+R + HGILSPTPGLL
Sbjct: 61  VQRLTGHHQPPSSSSNHALVHDHPPPDPISIPITDAIDDQAGGVDRIIAHGILSPTPGLL 120

Query: 121 PPISPNIFTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           PPISP IF+ P  PPQ    A  DL+  T FF DLSPI  NHFS SLDFFQQNFPDFH
Sbjct: 121 PPISPTIFSTP--PPQAAAAATGDLTSLTQFFHDLSPIPANHFSPSLDFFQQNFPDFH 176

BLAST of Lsi08G005310 vs. ExPASy TrEMBL
Match: A0A6J1FKV1 (protein MKS1-like OS=Cucurbita moschata OX=3662 GN=LOC111445144 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.6e-49
Identity = 119/171 (69.59%), Postives = 130/171 (76.02%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPPPHRPVIIYTVSPKIIHTEPTEFKDLVQ 60
           M ERK++QLQGPRPAALKITKDSHKI K    PPHRPVIIYTVSPK+IHT+PT+FKDLVQ
Sbjct: 1   MAERKELQLQGPRPAALKITKDSHKIGK----PPHRPVIIYTVSPKVIHTDPTQFKDLVQ 60

Query: 61  RLTGCRPSSSNDALDHPSDTTISSMSMSMPMSTD-QGVERVGHGILSPTPGLLPPISPNI 120
           RLTG +PSS +    HP +TT       M MST+ QG ERVGHGILSPTPGLLPPI  NI
Sbjct: 61  RLTGHKPSSHDS--HHPPNTT-------MIMSTNHQGTERVGHGILSPTPGLLPPIPANI 120

Query: 121 FTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           FTP P       P  A+LSP THFF+DLSPIA N FS SLDFF QNFPD H
Sbjct: 121 FTPTP-------PQSAELSPLTHFFRDLSPIAPNRFSLSLDFF-QNFPDLH 150

BLAST of Lsi08G005310 vs. ExPASy TrEMBL
Match: A0A1S3B4N1 (protein MKS1-like OS=Cucumis melo OX=3656 GN=LOC103485735 PE=4 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.6e-49
Identity = 118/176 (67.05%), Postives = 129/176 (73.30%), Query Frame = 0

Query: 3   ERKDVQLQGPRPAALKITKDSHKIKKPPLPP--PHRPVIIYTVSPKIIHTEPTEFKDLVQ 62
           +++ +QLQGPRP+ALKITKDSHKIKKPPLPP  PHRPVIIYTVSPKIIHT+PT+FKDLVQ
Sbjct: 35  KKRKLQLQGPRPSALKITKDSHKIKKPPLPPPQPHRPVIIYTVSPKIIHTDPTQFKDLVQ 94

Query: 63  RLTGCR---PSSSNDAL--DHPSDTTISSMSMSMPMSTDQGVER-VGHGILSPTPGLLPP 122
           RLTG      SSSN AL  DHP    IS            GV+R + HGILSPTPGLLPP
Sbjct: 95  RLTGHHQPPSSSSNHALVHDHPPPDPISIPITDAIDDQAGGVDRIIAHGILSPTPGLLPP 154

Query: 123 ISPNIFTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           ISP IF+ P  PPQ    A  DL+  T FF DLSPI  NHFS SLDFFQQNFPDFH
Sbjct: 155 ISPTIFSTP--PPQAAAAATGDLTSLTQFFHDLSPIPANHFSPSLDFFQQNFPDFH 208

BLAST of Lsi08G005310 vs. ExPASy TrEMBL
Match: A0A6J1JW33 (protein MKS1-like OS=Cucurbita maxima OX=3661 GN=LOC111489392 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 7.7e-49
Identity = 118/171 (69.01%), Postives = 129/171 (75.44%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPPPHRPVIIYTVSPKIIHTEPTEFKDLVQ 60
           M ERK+VQLQGPRPAALKITKDSHKI K    PPHRPVIIY+VSPK+IHT+PT+FKDLVQ
Sbjct: 1   MAERKEVQLQGPRPAALKITKDSHKIGK----PPHRPVIIYSVSPKVIHTDPTQFKDLVQ 60

Query: 61  RLTGCRPSSSNDALDHPSDTTISSMSMSMPMSTD-QGVERVGHGILSPTPGLLPPISPNI 120
           RLTG +PSS +    HP +TT       M MST+  G ERVGHGILSPTPGLLPPI  NI
Sbjct: 61  RLTGHKPSSHDS--HHPPNTT-------MIMSTNPHGAERVGHGILSPTPGLLPPIPANI 120

Query: 121 FTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           FTP P       P  A+LSP THFF+DLSPIA N FS SLDFF QNFPD H
Sbjct: 121 FTPTP-------PQSAELSPLTHFFRDLSPIAPNRFSLSLDFF-QNFPDLH 150

BLAST of Lsi08G005310 vs. NCBI nr
Match: XP_038885910.1 (protein MKS1-like [Benincasa hispida])

HSP 1 Score: 265.4 bits (677), Expect = 3.4e-67
Identity = 147/176 (83.52%), Postives = 149/176 (84.66%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPL----PPPHRPVIIYTVSPKIIHTEPTEFK 60
           MPERK+V LQGPRPAALKITKDSHKIKKPPL    PPPHRPVIIYTVSPKIIHTEPTEFK
Sbjct: 1   MPERKEVHLQGPRPAALKITKDSHKIKKPPLPPPHPPPHRPVIIYTVSPKIIHTEPTEFK 60

Query: 61  DLVQRLTGCRPSSSNDALDHPSDTTISSMSMSM-PMSTDQGVERVGHGILSPTPGLLPPI 120
           DLVQRLTG RPSSSNDALDHP     +SMSMSM   S DQGVERVGHGILSPTPGLLPPI
Sbjct: 61  DLVQRLTGLRPSSSNDALDHP-----TSMSMSMNTNSIDQGVERVGHGILSPTPGLLPPI 120

Query: 121 SPNIFTPPPLPPQTTMPAVA-DLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           S NIFTP   PPQ  M A A D SPFT FFQD SPIATNHFSSSLDFFQQNFPDFH
Sbjct: 121 SSNIFTP---PPQAAMAAAATDPSPFTQFFQDFSPIATNHFSSSLDFFQQNFPDFH 168

BLAST of Lsi08G005310 vs. NCBI nr
Match: XP_004148825.1 (protein MKS1 [Cucumis sativus])

HSP 1 Score: 219.2 bits (557), Expect = 2.8e-53
Identity = 124/179 (69.27%), Postives = 134/179 (74.86%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPP--PHRPVIIYTVSPKIIHTEPTEFKDL 60
           MPE+K++QLQGPRP+ALKITKDSHKIKKPPLPP  PHRPVIIYTVSPKIIHT+PTEFKDL
Sbjct: 1   MPEKKELQLQGPRPSALKITKDSHKIKKPPLPPPQPHRPVIIYTVSPKIIHTDPTEFKDL 60

Query: 61  VQRLTGCR---PSSSNDAL---DHPSDTTISSMSMSMPMSTDQGVER-VGHGILSPTPGL 120
           VQRLTG      SSSN AL    HP    IS  +   P+    GV+R + HGILSPTPGL
Sbjct: 61  VQRLTGHHQPPSSSSNHALVHDHHPPPDHISIPTTDDPIDDQAGVDRIIAHGILSPTPGL 120

Query: 121 LPPISPNIFTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           LPPIS  IF PPP  P     A  DL+  T FF DLSPIA NHFSSSLDFFQQNFPDFH
Sbjct: 121 LPPISSTIFNPPPQAPAAA--AAGDLTSLTQFFHDLSPIAANHFSSSLDFFQQNFPDFH 177

BLAST of Lsi08G005310 vs. NCBI nr
Match: KAA0058915.1 (protein MKS1-like [Cucumis melo var. makuwa])

HSP 1 Score: 213.8 bits (543), Expect = 1.2e-51
Identity = 122/178 (68.54%), Postives = 132/178 (74.16%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPP--PHRPVIIYTVSPKIIHTEPTEFKDL 60
           MPE+K++QLQGPRP+ALKITKDSHKIKKPPLPP  PHRPVIIYTVSPKIIHT+PT+FKDL
Sbjct: 1   MPEKKELQLQGPRPSALKITKDSHKIKKPPLPPPQPHRPVIIYTVSPKIIHTDPTQFKDL 60

Query: 61  VQRLTGCR---PSSSNDAL--DHPSDTTISSMSMSMPMSTDQGVER-VGHGILSPTPGLL 120
           VQRLTG      SSSN AL  DHP    IS            GV+R + HGILSPTPGLL
Sbjct: 61  VQRLTGHHQPPSSSSNHALVHDHPPPDPISIPITDAIDDQAGGVDRIIAHGILSPTPGLL 120

Query: 121 PPISPNIFTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           PPISP IF+ P  PPQ    A  DL+  T FF DLSPI  NHFS SLDFFQQNFPDFH
Sbjct: 121 PPISPTIFSTP--PPQAAAAATGDLTSLTQFFHDLSPIPANHFSPSLDFFQQNFPDFH 176

BLAST of Lsi08G005310 vs. NCBI nr
Match: KAG6578908.1 (Protein MKS1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016429.1 Protein MKS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 208.0 bits (528), Expect = 6.5e-50
Identity = 120/171 (70.18%), Postives = 131/171 (76.61%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPPPHRPVIIYTVSPKIIHTEPTEFKDLVQ 60
           M ERK++QLQGPRPAALKITKDSHKI K    PPHRPVIIYTVSPK+IHT+PT+FKDLVQ
Sbjct: 13  MAERKELQLQGPRPAALKITKDSHKIGK----PPHRPVIIYTVSPKVIHTDPTQFKDLVQ 72

Query: 61  RLTGCRPSSSNDALDHPSDTTISSMSMSMPMSTD-QGVERVGHGILSPTPGLLPPISPNI 120
           RLTG +PSS +    HP +TT       M MST+ QG ERVGHGILSPTPGLLPPI  NI
Sbjct: 73  RLTGHKPSSHDS--HHPPNTT-------MIMSTNHQGAERVGHGILSPTPGLLPPIPANI 132

Query: 121 FTPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           FTP P       P  A+LSP THFF+DLSPIA N FSSSLDFF QNFPD H
Sbjct: 133 FTPTP-------PQSAELSPLTHFFRDLSPIAPNRFSSSLDFF-QNFPDLH 162

BLAST of Lsi08G005310 vs. NCBI nr
Match: XP_023551504.1 (protein MKS1-like [Cucurbita pepo subsp. pepo] >XP_023551505.1 protein MKS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 206.8 bits (525), Expect = 1.4e-49
Identity = 118/170 (69.41%), Postives = 129/170 (75.88%), Query Frame = 0

Query: 1   MPERKDVQLQGPRPAALKITKDSHKIKKPPLPPPHRPVIIYTVSPKIIHTEPTEFKDLVQ 60
           M ERK+VQLQGPRPAALKITKDSHKI K    PPHRPVIIYTVSPK+IHT+PT+FKDLVQ
Sbjct: 1   MAERKEVQLQGPRPAALKITKDSHKIGK----PPHRPVIIYTVSPKVIHTDPTQFKDLVQ 60

Query: 61  RLTGCRPSSSNDALDHPSDTTISSMSMSMPMSTDQGVERVGHGILSPTPGLLPPISPNIF 120
           RLTG +P SS+D+   P+ T I S       +  QG ERVGHGILSPTPGLLPPI  NIF
Sbjct: 61  RLTGHKP-SSHDSHHSPNTTMIMS-------TNHQGAERVGHGILSPTPGLLPPIPTNIF 120

Query: 121 TPPPLPPQTTMPAVADLSPFTHFFQDLSPIATNHFSSSLDFFQQNFPDFH 171
           TP P       P  A+LSP THFF+DLSPIA N FSSSLDFF QNFPD H
Sbjct: 121 TPTP-------PQSAELSPLTHFFRDLSPIAPNRFSSSLDFF-QNFPDLH 150

BLAST of Lsi08G005310 vs. TAIR 10
Match: AT3G18690.1 (MAP kinase substrate 1 )

HSP 1 Score: 76.6 bits (187), Expect = 2.1e-14
Identity = 64/181 (35.36%), Postives = 85/181 (46.96%), Query Frame = 0

Query: 3   ERKDVQLQGPRPAALKITKDSHKIKKP---PLPPPHR----------PVIIYTVSPKIIH 62
           +++ +Q+ GPRP+ L + KDSHKIKKP   P PPP+R          PV+IY VSPK++H
Sbjct: 18  QKRQLQICGPRPSPLSVHKDSHKIKKPPKHPAPPPNRDQPPPYIPREPVVIYAVSPKVVH 77

Query: 63  TEPTEFKDLVQRLTGCRP---------SSSNDALDHPSDTTISSMSMSMPMSTDQGVE-- 122
              +EF ++VQRLTG               + A    S    S      P + D+ VE  
Sbjct: 78  ATASEFMNVVQRLTGISSGVFLESGGGGDVSPAARLASTENASPRGGKEPAARDETVEIN 137

Query: 123 ----------RVGHGILSPTPGLLPPISPNIFTP---------PPLPPQTTMPAVADLSP 141
                         GILSP+P LLP  S  IF+P         P +P     PA   +SP
Sbjct: 138 TAMEEAAEFGGYAPGILSPSPALLPTASTGIFSPMYHQGGMFSPAIPLGLFSPA-GFMSP 197

BLAST of Lsi08G005310 vs. TAIR 10
Match: AT1G21320.1 (nucleotide binding;nucleic acid binding )

HSP 1 Score: 72.0 bits (175), Expect = 5.1e-13
Identity = 50/118 (42.37%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 11  GPRPAALKITKDSHK-IKKPPLPPPH--------------------RPVIIYTVSPKIIH 70
           GP+P  LK+  DSHK IKKPPL PPH                     PV IYTV+P+IIH
Sbjct: 13  GPKPTPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPSQSRPPPGPVNIYTVTPRIIH 72

Query: 71  TEPTEFKDLVQRLTGCRPSSSNDALDHPSDTTISSMSMSMPMSTDQGVERVGHGILSP 108
           T P  F  LVQRLTG   +S+         ++ SS S S P  T   V+   HG++SP
Sbjct: 73  THPNNFMTLVQRLTGQTSTSTT--------SSSSSSSTSEPKDTSTMVD-TSHGLISP 121

BLAST of Lsi08G005310 vs. TAIR 10
Match: AT1G21326.1 (VQ motif-containing protein )

HSP 1 Score: 68.9 bits (167), Expect = 4.4e-12
Identity = 76/236 (32.20%), Postives = 92/236 (38.98%), Query Frame = 0

Query: 11  GPRPAALKITKDSHK-IKKPPLPPPH--------------------RPVIIYTVSPKIIH 70
           GPRP  LK+  DSHK IKKPPL PPH                     PVIIYTVSP+IIH
Sbjct: 13  GPRPIPLKVRGDSHKIIKKPPLAPPHPQPQPPQTHQQEPSQSRPPPGPVIIYTVSPRIIH 72

Query: 71  TEPTEFKDLVQRLTGCRPSSSNDALDHPSDTTISSMSMSM-------------------- 130
           T P  F  LVQRLTG + S+S  +  + S T+    + +M                    
Sbjct: 73  THPNNFMTLVQRLTG-KTSTSTTSSSYSSSTSAPKDASTMVDTSHGLISPAARFAVTEKA 132

Query: 131 ------------------------------PMSTDQGVERVGH---GILSPTPGLLPPIS 167
                                             +QG ER      GILSP P  LP +S
Sbjct: 133 NISNELGTFVGGEGTMDQYYHYHHHHHHQEQQHQNQGFERPSFHHAGILSPGPNSLPSVS 192

BLAST of Lsi08G005310 vs. TAIR 10
Match: AT3G18360.1 (VQ motif-containing protein )

HSP 1 Score: 65.1 bits (157), Expect = 6.3e-11
Identity = 35/67 (52.24%), Postives = 41/67 (61.19%), Query Frame = 0

Query: 12  PRPAALKITKDSHKIKKPPLP-------PPHRPVIIYTVSPKIIHTEPTEFKDLVQRLTG 71
           P P  LK+ KDSH IKKPP P        P  PVIIYT +P+IIHT P +F  LVQ+LTG
Sbjct: 41  PSPPTLKVNKDSHVIKKPPSPSSFSSAAKPRHPVIIYTHTPRIIHTNPKDFMALVQKLTG 100

BLAST of Lsi08G005310 vs. TAIR 10
Match: AT1G68450.1 (VQ motif-containing protein )

HSP 1 Score: 51.2 bits (121), Expect = 9.4e-07
Identity = 45/128 (35.16%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 8   QLQGPRPAALKITKDSHKIKK----PPLPPPH---RPVIIYTVSPKIIHTEPTEFKDLVQ 67
           ++ G RP++LK+  +SH IKK       P PH    PVIIY  SPK+IHT   +F  LVQ
Sbjct: 8   EINGSRPSSLKLAGESHTIKKTSSCKSKPRPHGRASPVIIYAHSPKVIHTRAEDFMALVQ 67

Query: 68  RLTGC-----RPSSSNDALDHPSDTTISSMSMSMPMSTDQGVERVGHGILSPTPGLLPPI 124
           RLTG      R +S + +     +  +   + + P S D    R     L+  P L  P 
Sbjct: 68  RLTGLDEIIRRNTSESSSSVVTEEVNVGDDNTAAPFSQD----RTQRQKLTDMP-LFTPS 127

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LGD52.9e-1335.36Protein MKS1 OS=Arabidopsis thaliana OX=3702 GN=MKS1 PE=1 SV=2[more]
F4HWF97.2e-1242.37Nuclear speckle RNA-binding protein B OS=Arabidopsis thaliana OX=3702 GN=NSRB PE... [more]
Q9LS548.9e-1052.24VQ motif-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=VQ20 PE=2 SV=1[more]
Q9CA361.3e-0535.16VQ motif-containing protein 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0KI801.4e-5369.27VQ domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G338080 PE=4 SV=... [more]
A0A5A7UZA15.7e-5268.54Protein MKS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold98G008... [more]
A0A6J1FKV11.6e-4969.59protein MKS1-like OS=Cucurbita moschata OX=3662 GN=LOC111445144 PE=4 SV=1[more]
A0A1S3B4N11.6e-4967.05protein MKS1-like OS=Cucumis melo OX=3656 GN=LOC103485735 PE=4 SV=1[more]
A0A6J1JW337.7e-4969.01protein MKS1-like OS=Cucurbita maxima OX=3661 GN=LOC111489392 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038885910.13.4e-6783.52protein MKS1-like [Benincasa hispida][more]
XP_004148825.12.8e-5369.27protein MKS1 [Cucumis sativus][more]
KAA0058915.11.2e-5168.54protein MKS1-like [Cucumis melo var. makuwa][more]
KAG6578908.16.5e-5070.18Protein MKS1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016429.1 Prot... [more]
XP_023551504.11.4e-4969.41protein MKS1-like [Cucurbita pepo subsp. pepo] >XP_023551505.1 protein MKS1-like... [more]
Match NameE-valueIdentityDescription
AT3G18690.12.1e-1435.36MAP kinase substrate 1 [more]
AT1G21320.15.1e-1342.37nucleotide binding;nucleic acid binding [more]
AT1G21326.14.4e-1232.20VQ motif-containing protein [more]
AT3G18360.16.3e-1152.24VQ motif-containing protein [more]
AT1G68450.19.4e-0735.16VQ motif-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008889VQPFAMPF05678VQcoord: 42..65
e-value: 6.2E-11
score: 41.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availablePANTHERPTHR33143:SF6F16F4.1 PROTEINcoord: 6..75
IPR039607VQ motif-containing protein 8/17/18/20/21/25PANTHERPTHR33143F16F4.1 PROTEIN-RELATEDcoord: 6..75

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G005310.1Lsi08G005310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
cellular_component GO:0005634 nucleus