Lsi08G005120 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G005120
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Basic blue protein-like
Location	chr08: 13039771 .. 13041817 (-)
RNA-Seq Expression	Lsi08G005120
Synteny	Lsi08G005120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCACTCTCTCCCATTGCCATTTGTTTCAATATAATATAATTCTTTCCCAAAAGAAAAAAGAAAAAGAAAAGAAGAAAAATGGGAATTATGGTGGGTGAGGGAAGAGGCAGTGCAGTCGGAAGAGCCGTCGCTGTTGCCGCCGTTATCGGGTTGGTTATGATGTCTCAGTTGGAGAACGTGGAGGCAGCCGTTTACGTTGTCGGCGGCTCCGGTGGATGGACTTTTAACGTCCAAAGTTGGCCTCAAGGAAAACGTTTTCGTGCCGGTGATATTCTTCGTAAGTCCTTCCCACCACATTTTCAACTTGTTTAAAAATATAAATACTTAAATCTTTGCTCTCTCACTCTTTTAGCTATAAAAAAAAACTTGTGCAGTGCAAATTGCAAAGACATTAACTAGTTTAAATGGATTAAAAAATTAATTATTATTTTTATTTTTTTTCTACTCTAAATAAAAAAAAGATACATGAAATTACTTACAGCCATTTTAAGTATAGTTTCTATTCATTTACTTCTTAAATTTCAAAATATTACATTTTATCGTGGAATTTTATTTTCATTTTATTTTTAAGTTTTGAATTTATATTTTGAATTTTGATTTTTTAATTAAATAATTAGACTAATTTAGTTAAAGTTAAGATATCATTTTGTTCTTGTACCAGTTGAGCTATTTTCAAGTTAGAATATATATTCATATTTTTTTAAAATTATTGTCATTATGTAACCATTATCGATAAATATTACTAACTTATATATATATATATATATATATATATATTCAAAAAAAATCAACAATAAACTAACATAGATTAAAAGTATGTTTGGATTGACTTTCTAAATGTTTATAAACGCTTTTTCTAAACGCACTCTAGATTTATGATTTATTGAAAGTACATTGAAACAAGTCTCAAAGTATAAGAACCAACATGATATTCTAACCTTTAATATAATATAATTAAAAATTAATGTTTCCTACATTTTAATGTGAGGCCACTACAAACTAAAAGTAAGCCGGAGTGAAAGCTTGGTAAAAGTGTAAAACAAAAGACATTAAATTAGATTAAAAATCATTTTTAGTCCCTAAATTTTTATAAAAGTAACAATTTTGTCTATGAACTTTGGTTTGCAATGATTTAGTCACTGTACTTTCAATATTGTGACAATTTAGTCTTTGGATTTTGCTATGTAATAATTTAGTCATTGTACTTTAAAATTTATAATAATTTAGTCTCTATTATAAAAATTTGTGTTCAAATTTAATGAGATTTCTTTCCGTAAATAAACCAATAAACTAATTAAAGGCTCAACATTTTTATAAAATACAAAGTTACATCATAAAATAATAAAAATTTATATCAAATTTTTTTTCCGTTGGGACTAAGCTATTACAAATTTGAAGTACAAAGTTTAAATGATTACATCCTAAAGTTCATAGAGAATAAAAGTGATTTTTAACCTAAATTAAACCCAAAAACTTGGGAATTAAAAGGTTAACATTTTTTAGACGAAAATAGTATTTTGGTATTTATTGGAAAAATAAAAATAGTGGTGGAAATTTGTTTGCAGAGTTCAATTACGACTCAACAATTCATAACGTGGTGGTGGTGAACCCAGCTGGGTACGCCAACTGCAGAACTCCGGCGGGTGCCAAAGTGTACAAATCCGGTAGTGATCAGATAAAGTTGCCAAAAGGAAAAAGTTATTTCATCTGCAATTTCCCCGGCCACTGCGAGTCCGGCATGAAGATTGCCGTCGATGCTCTCTAAAATTTCCAACTTCCAACCAACTTTTACTTTATTTACTACTGTTAAAATAAAAAAAAGTAACTGCGGTCCTTCTTTACTTTATATGGTGGGAGTGTTTTTTTTCCTTTCTTCTAGTTGATATGTCATTTTCCTTGTTCCAAACTTATATAGGTCATTTGATCATATCAATTGCCTTGTTTCTTTGCTCTTTTCGAGGGTGTTTTGAGGGGAGAAAATTTCTAGTATTATAATAGTTATATGGAAAATTCTACGTTTATTTCTTGCTACAATAGTAATATTTAA

mRNA sequence

TCACTCTCTCCCATTGCCATTTGTTTCAATATAATATAATTCTTTCCCAAAAGAAAAAAGAAAAAGAAAAGAAGAAAAATGGGAATTATGGTGGGTGAGGGAAGAGGCAGTGCAGTCGGAAGAGCCGTCGCTGTTGCCGCCGTTATCGGGTTGGTTATGATGTCTCAGTTGGAGAACGTGGAGGCAGCCGTTTACGTTGTCGGCGGCTCCGGTGGATGGACTTTTAACGTCCAAAGTTGGCCTCAAGGAAAACGTTTTCGTGCCGGTGATATTCTTCAGTTCAATTACGACTCAACAATTCATAACGTGGTGGTGGTGAACCCAGCTGGGTACGCCAACTGCAGAACTCCGGCGGGTGCCAAAGTGTACAAATCCGGTAGTGATCAGATAAAGTTGCCAAAAGGAAAAAGTTATTTCATCTGCAATTTCCCCGGCCACTGCGAGTCCGGCATGAAGATTGCCGTCGATGCTCTCTAAAATTTCCAACTTCCAACCAACTTTTACTTTATTTACTACTGTTAAAATAAAAAAAAGTAACTGCGGTCCTTCTTTACTTTATATGGTGGGAGTGTTTTTTTTCCTTTCTTCTAGTTGATATGTCATTTTCCTTGTTCCAAACTTATATAGGTCATTTGATCATATCAATTGCCTTGTTTCTTTGCTCTTTTCGAGGGTGTTTTGAGGGGAGAAAATTTCTAGTATTATAATAGTTATATGGAAAATTCTACGTTTATTTCTTGCTACAATAGTAATATTTAA

Coding sequence (CDS)

ATGGGAATTATGGTGGGTGAGGGAAGAGGCAGTGCAGTCGGAAGAGCCGTCGCTGTTGCCGCCGTTATCGGGTTGGTTATGATGTCTCAGTTGGAGAACGTGGAGGCAGCCGTTTACGTTGTCGGCGGCTCCGGTGGATGGACTTTTAACGTCCAAAGTTGGCCTCAAGGAAAACGTTTTCGTGCCGGTGATATTCTTCAGTTCAATTACGACTCAACAATTCATAACGTGGTGGTGGTGAACCCAGCTGGGTACGCCAACTGCAGAACTCCGGCGGGTGCCAAAGTGTACAAATCCGGTAGTGATCAGATAAAGTTGCCAAAAGGAAAAAGTTATTTCATCTGCAATTTCCCCGGCCACTGCGAGTCCGGCATGAAGATTGCCGTCGATGCTCTCTAA

Protein sequence

MGIMVGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFRAGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHCESGMKIAVDAL

Homology

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BLAST of Lsi08G005120 vs. ExPASy Swiss-Prot
Match: P00303 (Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 8.1e-43
Identity = 77/96 (80.21%), Postives = 86/96 (89.58%), Query Frame = 0


Query: 37  AVYVVGGSGGWTFNVQSWPQGKRFRAGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKV 96
           AVYVVGGSGGWTFN +SWP+GKRFRAGDIL FNY+  +HNVVVVN  G++ C TPAGAKV
Sbjct: 1   AVYVVGGSGGWTFNTESWPKGKRFRAGDILLFNYNPXMHNVVVVNQGGFSTCNTPAGAKV 60

Query: 97  YKSGSDQIKLPKGKSYFICNFPGHCESGMKIAVDAL 133
           Y SG DQIKLPKG+SYFICNFPGHC+SGMKIAV+AL
Sbjct: 61  YTSGRDQIKLPKGQSYFICNFPGHCQSGMKIAVNAL 96

[Close]

BLAST of Lsi08G005120 vs. ExPASy Swiss-Prot
Match: Q8LG89 (Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2)

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-35
Identity = 71/129 (55.04%), Postives = 92/129 (71.32%), Query Frame = 0


Query: 5   VGEGRGSAVGRAVAVAAVIGL-VMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFRAG 64
           + +GRGSA   A A+  ++ + V++ Q + V+AA Y VG SG WTFN   WP+GK FRAG
Sbjct: 1   MAKGRGSASWSARAIVTLMAVSVLLLQADYVQAATYTVGDSGIWTFNAVGWPKGKHFRAG 60

Query: 65  DILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHCES 124
           D+L FNY+  +HNVV V+   Y NC+TP GAK Y SG D+I L KG+++FICNFP HCES
Sbjct: 61  DVLVFNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHCES 120

Query: 125 GMKIAVDAL 133
            MKIAV A+
Sbjct: 121 DMKIAVTAV 129

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BLAST of Lsi08G005120 vs. ExPASy Swiss-Prot
Match: P60496 (Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-29
Identity = 58/123 (47.15%), Postives = 77/123 (62.60%), Query Frame = 0


Query: 9   RGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFRAGDILQF 68
           +GS       V  V+ + ++   E  E+ VY VG  GGWTF    WP GK FRAGD+L F
Sbjct: 3   QGSGSAERALVLGVVLVFLVFNCEVAESVVYTVGDGGGWTFGTSGWPAGKTFRAGDVLVF 62

Query: 69  NYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHCESGMKIA 128
            Y+  +HNVV V   GY +C    G++V+KSG D+I L +G +YFIC+ PGHC+ G+KIA
Sbjct: 63  KYNPAVHNVVSVPAGGYKSCTASPGSRVFKSGDDRITLSRGTNYFICSVPGHCQGGLKIA 122

Query: 129 VDA 132
           V A
Sbjct: 123 VTA 125

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BLAST of Lsi08G005120 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 1.4e-15
Identity = 38/99 (38.38%), Postives = 56/99 (56.57%), Query Frame = 0


Query: 36  AAVYVVGGSGGWT----FNVQSWPQGKRFRAGDILQFNYDSTIHNVVVVNPAGYANCRTP 95
           A V+ VG S GWT    ++   W    +F  GD L FNY++  HNV+ V+   + +C + 
Sbjct: 1   ATVHKVGDSTGWTTLVPYDYAKWASSNKFHVGDSLLFNYNNKFHNVLQVDQEQFKSCNSS 60

Query: 96  AGAKVYKSGSDQIKLPK-GKSYFICNFPGHCESGMKIAV 130
           + A  Y SG+D I L + G  YF+C  PGHC+ G K+ +
Sbjct: 61  SPAASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEI 99

[Close]

BLAST of Lsi08G005120 vs. ExPASy Swiss-Prot
Match: Q96316 (Uclacyanin-3 OS=Arabidopsis thaliana OX=3702 GN=UCC3 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-14
Identity = 48/123 (39.02%), Postives = 68/123 (55.28%), Query Frame = 0


Query: 13  VGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQ--SWPQGKRFRAGDILQFNY 72
           +G  VA A    L+ ++ +  V AA + VG   GWT N+    W  GK FR GD L+F Y
Sbjct: 1   MGSTVAAAL---LLFLAAVPAVFAATFKVGDISGWTSNLDYTVWLTGKTFRVGDTLEFVY 60

Query: 73  DSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPK-GKSYFICNFPGHCESGMKIAV 132
             + H+V VV+ AGY NC +    + +  G  +I L   G  +F+C   GHC++GMK+AV
Sbjct: 61  GLS-HSVSVVDKAGYDNCDSSGATQNFADGDTKIDLTTVGTMHFLCPTFGHCKNGMKLAV 119

[Close]

BLAST of Lsi08G005120 vs. ExPASy TrEMBL
Match: A0A5A7UXE1 (Basic blue protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1607G00980 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.9e-58
Identity = 112/132 (84.85%), Postives = 124/132 (93.94%), Query Frame = 0


Query: 1   MGIMVGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRF 60
           MGI VGEGRGSA+GR VAVAAVIGLVMMSQLE+VEAAVY VGGS GWTFNVQ+WP+G RF
Sbjct: 1   MGIKVGEGRGSAIGRGVAVAAVIGLVMMSQLESVEAAVYDVGGSSGWTFNVQNWPKGIRF 60

Query: 61  RAGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGH 120
           RAGDIL+FNYDS+IHNVVVVN AGYA+C+TP GAKVY +GSDQIKLPKG+SYFICNFPGH
Sbjct: 61  RAGDILKFNYDSSIHNVVVVNKAGYASCKTPGGAKVYNTGSDQIKLPKGQSYFICNFPGH 120

Query: 121 CESGMKIAVDAL 133
           CE+GMKIAV+AL
Sbjct: 121 CEAGMKIAVNAL 132

[Close]

BLAST of Lsi08G005120 vs. ExPASy TrEMBL
Match: A0A1S3B4N7 (basic blue protein-like OS=Cucumis melo OX=3656 GN=LOC103485741 PE=4 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 7.0e-58
Identity = 111/131 (84.73%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 2   GIMVGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFR 61
           GI VGEGRGSA+GR VAVAAVIGLVMMSQLE+VEAAVY VGGS GWTFNVQ+WP+G RFR
Sbjct: 15  GIKVGEGRGSAIGRGVAVAAVIGLVMMSQLESVEAAVYDVGGSSGWTFNVQNWPKGIRFR 74

Query: 62  AGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHC 121
           AGDIL+FNYDS+IHNVVVVN AGYA+C+TP GAKVY +GSDQIKLPKG+SYFICNFPGHC
Sbjct: 75  AGDILKFNYDSSIHNVVVVNKAGYASCKTPGGAKVYNTGSDQIKLPKGQSYFICNFPGHC 134

Query: 122 ESGMKIAVDAL 133
           E+GMKIAV+AL
Sbjct: 135 EAGMKIAVNAL 145

[Close]

BLAST of Lsi08G005120 vs. ExPASy TrEMBL
Match: A0A0A0KCU9 (Basic blue protein OS=Cucumis sativus OX=3659 GN=Csa_6G344240 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.0e-57
Identity = 110/132 (83.33%), Postives = 122/132 (92.42%), Query Frame = 0


Query: 1   MGIMVGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRF 60
           MGI VGEGRGSA+ RAVAVAAVIGLVMMSQLE+VEAAVY VGGSGGWTFN +SWP+GKRF
Sbjct: 1   MGIKVGEGRGSAIERAVAVAAVIGLVMMSQLESVEAAVYDVGGSGGWTFNTESWPKGKRF 60

Query: 61  RAGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGH 120
           RAGDIL+FNY+  +HNVVVVN  G++ C TPAGAKVYKSGSDQIKLPKG+SYFICNFPGH
Sbjct: 61  RAGDILRFNYNPLVHNVVVVNQGGFSTCNTPAGAKVYKSGSDQIKLPKGQSYFICNFPGH 120

Query: 121 CESGMKIAVDAL 133
           C+SGMKIAV+AL
Sbjct: 121 CQSGMKIAVNAL 132

[Close]

BLAST of Lsi08G005120 vs. ExPASy TrEMBL
Match: A0A6J1FJW8 (basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444901 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.2e-51
Identity = 101/125 (80.80%), Postives = 109/125 (87.20%), Query Frame = 0


Query: 8   GRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFRAGDILQ 67
           GRGSAVG A+ VAAVIGLVMMSQLE+VEAAVY VG +GGWTFNVQ WP GK FRAGD L+
Sbjct: 2   GRGSAVGSALVVAAVIGLVMMSQLESVEAAVYTVGDAGGWTFNVQGWPNGKHFRAGDTLK 61

Query: 68  FNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHCESGMKI 127
           FNYD TIHNVVVVN  GY +C TPAGAKV+ SGSDQIKL KG +YFICNFPGHCESGMKI
Sbjct: 62  FNYDRTIHNVVVVNQGGYTSCLTPAGAKVFTSGSDQIKLAKGMNYFICNFPGHCESGMKI 121

Query: 128 AVDAL 133
           AV+AL
Sbjct: 122 AVNAL 126

[Close]

BLAST of Lsi08G005120 vs. ExPASy TrEMBL
Match: A0A6J1JXQ8 (basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111489786 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.9e-51
Identity = 101/125 (80.80%), Postives = 107/125 (85.60%), Query Frame = 0


Query: 8   GRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFRAGDILQ 67
           GRGSAVG A+ VAAVIGLVMMSQLENVEAAVY VG SGGWTFNVQ WP GK FRAGD L+
Sbjct: 2   GRGSAVGSALVVAAVIGLVMMSQLENVEAAVYTVGDSGGWTFNVQGWPNGKLFRAGDTLK 61

Query: 68  FNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHCESGMKI 127
           FNY+   HNVVVVN  GY +CRTP GAKV+ SGSDQIKL KG SYFICNFPGHCESGMKI
Sbjct: 62  FNYNPKFHNVVVVNQGGYTSCRTPVGAKVFTSGSDQIKLTKGMSYFICNFPGHCESGMKI 121

Query: 128 AVDAL 133
           AV+AL
Sbjct: 122 AVNAL 126

[Close]

BLAST of Lsi08G005120 vs. NCBI nr
Match: KAA0058846.1 (basic blue protein-like [Cucumis melo var. makuwa] >TYK23773.1 basic blue protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 235.0 bits (598), Expect = 3.8e-58
Identity = 112/132 (84.85%), Postives = 124/132 (93.94%), Query Frame = 0


Query: 1   MGIMVGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRF 60
           MGI VGEGRGSA+GR VAVAAVIGLVMMSQLE+VEAAVY VGGS GWTFNVQ+WP+G RF
Sbjct: 1   MGIKVGEGRGSAIGRGVAVAAVIGLVMMSQLESVEAAVYDVGGSSGWTFNVQNWPKGIRF 60

Query: 61  RAGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGH 120
           RAGDIL+FNYDS+IHNVVVVN AGYA+C+TP GAKVY +GSDQIKLPKG+SYFICNFPGH
Sbjct: 61  RAGDILKFNYDSSIHNVVVVNKAGYASCKTPGGAKVYNTGSDQIKLPKGQSYFICNFPGH 120

Query: 121 CESGMKIAVDAL 133
           CE+GMKIAV+AL
Sbjct: 121 CEAGMKIAVNAL 132

[Close]

BLAST of Lsi08G005120 vs. NCBI nr
Match: XP_004144257.3 (basic blue protein-like [Cucumis sativus] >KAE8647192.1 hypothetical protein Csa_018915 [Cucumis sativus])

HSP 1 Score: 234.6 bits (597), Expect = 5.0e-58
Identity = 111/132 (84.09%), Postives = 123/132 (93.18%), Query Frame = 0


Query: 1   MGIMVGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRF 60
           MGI VGEGRGSA+GRAVAVAAVIGLVMMSQLE+VEAAVY VGGSGGWTFN +SWP+GKRF
Sbjct: 1   MGIKVGEGRGSAIGRAVAVAAVIGLVMMSQLESVEAAVYDVGGSGGWTFNTESWPKGKRF 60

Query: 61  RAGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGH 120
           RAGDIL+FNY+  +HNVVVVN  G++ C TPAGAKVYKSGSDQIKLPKG+SYFICNFPGH
Sbjct: 61  RAGDILRFNYNPLVHNVVVVNQGGFSTCNTPAGAKVYKSGSDQIKLPKGQSYFICNFPGH 120

Query: 121 CESGMKIAVDAL 133
           C+SGMKIAV+AL
Sbjct: 121 CQSGMKIAVNAL 132

[Close]

BLAST of Lsi08G005120 vs. NCBI nr
Match: XP_038885676.1 (basic blue protein-like [Benincasa hispida])

HSP 1 Score: 233.8 bits (595), Expect = 8.5e-58
Identity = 115/133 (86.47%), Postives = 122/133 (91.73%), Query Frame = 0


Query: 1   MGIM-VGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKR 60
           MGI+ VGEGRGSAVGRAV VAAVIGLVMMSQ E+VEA VYVVG S GWTFNVQ WP+GKR
Sbjct: 1   MGIIKVGEGRGSAVGRAVVVAAVIGLVMMSQFESVEAVVYVVGDSRGWTFNVQDWPKGKR 60

Query: 61  FRAGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPG 120
           FRAGDIL+FNY+ T HNVVVVN  GYA+CRTPAGAKVYKSGSDQIKLPKGKS+FICNFPG
Sbjct: 61  FRAGDILKFNYNPTFHNVVVVNQVGYASCRTPAGAKVYKSGSDQIKLPKGKSFFICNFPG 120

Query: 121 HCESGMKIAVDAL 133
           HCESGMKIAVDAL
Sbjct: 121 HCESGMKIAVDAL 133

[Close]

BLAST of Lsi08G005120 vs. NCBI nr
Match: XP_008441659.2 (PREDICTED: basic blue protein-like [Cucumis melo])

HSP 1 Score: 233.0 bits (593), Expect = 1.5e-57
Identity = 111/131 (84.73%), Postives = 123/131 (93.89%), Query Frame = 0


Query: 2   GIMVGEGRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFR 61
           GI VGEGRGSA+GR VAVAAVIGLVMMSQLE+VEAAVY VGGS GWTFNVQ+WP+G RFR
Sbjct: 15  GIKVGEGRGSAIGRGVAVAAVIGLVMMSQLESVEAAVYDVGGSSGWTFNVQNWPKGIRFR 74

Query: 62  AGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHC 121
           AGDIL+FNYDS+IHNVVVVN AGYA+C+TP GAKVY +GSDQIKLPKG+SYFICNFPGHC
Sbjct: 75  AGDILKFNYDSSIHNVVVVNKAGYASCKTPGGAKVYNTGSDQIKLPKGQSYFICNFPGHC 134

Query: 122 ESGMKIAVDAL 133
           E+GMKIAV+AL
Sbjct: 135 EAGMKIAVNAL 145

[Close]

BLAST of Lsi08G005120 vs. NCBI nr
Match: XP_023550997.1 (basic blue protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 214.2 bits (544), Expect = 7.0e-52
Identity = 102/125 (81.60%), Postives = 110/125 (88.00%), Query Frame = 0


Query: 8   GRGSAVGRAVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFRAGDILQ 67
           GRGSAVG A+ VAAVIGLVMMSQLE+VEAAVY VG +GGWTFNVQ WP GK FRAGD L+
Sbjct: 2   GRGSAVGSALVVAAVIGLVMMSQLESVEAAVYTVGDAGGWTFNVQGWPNGKHFRAGDTLK 61

Query: 68  FNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHCESGMKI 127
           FNYD TIHNVVVVN  GY +CRTPAGAKV+ SGSDQIKL KG +YFICNFPGHCESGMKI
Sbjct: 62  FNYDRTIHNVVVVNQGGYTSCRTPAGAKVFTSGSDQIKLAKGMNYFICNFPGHCESGMKI 121

Query: 128 AVDAL 133
           AV+AL
Sbjct: 122 AVNAL 126

[Close]

BLAST of Lsi08G005120 vs. TAIR 10
Match: AT2G02850.1 (plantacyanin )

HSP 1 Score: 150.2 bits (378), Expect = 1.2e-36
Identity = 71/129 (55.04%), Postives = 92/129 (71.32%), Query Frame = 0


Query: 5   VGEGRGSAVGRAVAVAAVIGL-VMMSQLENVEAAVYVVGGSGGWTFNVQSWPQGKRFRAG 64
           + +GRGSA   A A+  ++ + V++ Q + V+AA Y VG SG WTFN   WP+GK FRAG
Sbjct: 1   MAKGRGSASWSARAIVTLMAVSVLLLQADYVQAATYTVGDSGIWTFNAVGWPKGKHFRAG 60

Query: 65  DILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNFPGHCES 124
           D+L FNY+  +HNVV V+   Y NC+TP GAK Y SG D+I L KG+++FICNFP HCES
Sbjct: 61  DVLVFNYNPRMHNVVKVDSGSYNNCKTPTGAKPYTSGKDRITLSKGQNFFICNFPNHCES 120

Query: 125 GMKIAVDAL 133
            MKIAV A+
Sbjct: 121 DMKIAVTAV 129

[Close]

BLAST of Lsi08G005120 vs. TAIR 10
Match: AT1G17800.1 (early nodulin-like protein 22 )

HSP 1 Score: 89.7 bits (221), Expect = 1.9e-18
Identity = 52/132 (39.39%), Postives = 72/132 (54.55%), Query Frame = 0


Query: 8   GRGSAVGRAVAVAAVIGLVMMSQLENVEAA----VYVVGGSGGW--TFNVQSWPQGKRFR 67
           G  S V   V +A V+ ++ +     V  A     Y+VGG  GW     + +W +GK F 
Sbjct: 6   GHVSYVAVTVPIAIVMTVLCLFLANAVTYARRPTTYIVGGDDGWDPVVPMDTWARGKTFY 65

Query: 68  AGDILQFNYDSTIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPKGKSYFICNF-PGH 127
           AGDIL+F YD    N++VVN  GY  C    GA  Y SG D+I+L  G +YFI  + P  
Sbjct: 66  AGDILEFKYDYQRFNLIVVNRTGYETCEANVGAIEYSSGDDKIQLNYGYNYFIGTYTPED 125

Query: 128 CESGMKIAVDAL 133
           C +G+K+A+ AL
Sbjct: 126 CTTGLKMAIKAL 137

[Close]

BLAST of Lsi08G005120 vs. TAIR 10
Match: AT3G60270.1 (Cupredoxin superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.0e-17
Identity = 47/120 (39.17%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 16  AVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWTFNVQ--SWPQGKRFRAGDILQFNYDST 75
           + A  A++ L+++  +  V A  + VG + GWT  V+  SW   K FR GD L+F Y  +
Sbjct: 3   STAATALLLLLLLVAVPAVFAVTFQVGDNDGWTIGVEYTSWVSEKTFRVGDTLEFKYGPS 62

Query: 76  IHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPK-GKSYFICNFPGHCESGMKIAVDAL 133
            H+V VVN A Y  C T    + +  G  +I L K G  +F+C  PGHC  GMK+AV  L
Sbjct: 63  -HSVAVVNKADYDGCETSRPTQSFSDGDTKIDLTKVGAIHFLCLTPGHCSLGMKLAVQVL 121

[Close]

BLAST of Lsi08G005120 vs. TAIR 10
Match: AT3G17675.1 (Cupredoxin superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.3e-16
Identity = 38/99 (38.38%), Postives = 55/99 (55.56%), Query Frame = 0


Query: 35  EAAVYVVGGSGGWTF--NVQSWPQGKRFRAGDILQFNYDSTIHNVVVVNPAGYANCRTPA 94
           E   ++VG S GW    N  +W QG+ F  GD+L FNY S  HNV+ VN   Y +C    
Sbjct: 5   EGTEHIVGDSNGWELFTNYTNWTQGREFHVGDVLVFNYKSDQHNVMQVNSTAYTDCGLDN 64

Query: 95  GAKVYKSGSDQIKLPK-GKSYFICNFPGHCESGMKIAVD 131
              ++  G+D I L + GK +FIC    HC +G K++++
Sbjct: 65  YTTLFTKGNDSIILSEVGKLWFICGVDDHCVNGQKLSIN 103

[Close]

BLAST of Lsi08G005120 vs. TAIR 10
Match: AT5G26330.1 (Cupredoxin superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 3.9e-16
Identity = 46/122 (37.70%), Postives = 64/122 (52.46%), Query Frame = 0


Query: 16  AVAVAAVIGLVMMSQLENVEAAVYVVGGSGGWT----FNVQSWPQGKRFRAGDILQFNYD 75
           A+ VAA+  +V+M +L   EAAVY VG S GWT     + + W   K F  GD + F Y+
Sbjct: 3   AIIVAALACIVVMLRLS--EAAVYKVGDSAGWTTIANVDYKLWASTKTFHIGDTVLFEYN 62

Query: 76  STIHNVVVVNPAGYANCRTPAGAKVYKSGSDQIKLPK-GKSYFICNFPGHCESGMKIAVD 133
              HNV+ V    Y +C T      + +G+D I L   G  +F C  PGHC +G K+ + 
Sbjct: 63  PQFHNVMRVTHPMYRSCNTSKPISTFTTGNDSITLTNHGHHFFFCGVPGHCLAGQKLDLH 122

The following BLAST results are available for this feature:

BLAST of Lsi08G005120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P00303	8.1e-43	80.21	Basic blue protein OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
Q8LG89	1.6e-35	55.04	Basic blue protein OS=Arabidopsis thaliana OX=3702 GN=ARPN PE=2 SV=2	[more]
P60496	1.0e-29	47.15	Chemocyanin OS=Lilium longiflorum OX=4690 PE=1 SV=1	[more]
P80728	1.4e-15	38.38	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
Q96316	2.7e-14	39.02	Uclacyanin-3 OS=Arabidopsis thaliana OX=3702 GN=UCC3 PE=2 SV=1	[more]

BLAST of Lsi08G005120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UXE1	1.9e-58	84.85	Basic blue protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3B4N7	7.0e-58	84.73	basic blue protein-like OS=Cucumis melo OX=3656 GN=LOC103485741 PE=4 SV=1	[more]
A0A0A0KCU9	2.0e-57	83.33	Basic blue protein OS=Cucumis sativus OX=3659 GN=Csa_6G344240 PE=4 SV=1	[more]
A0A6J1FJW8	2.2e-51	80.80	basic blue protein-like OS=Cucurbita moschata OX=3662 GN=LOC111444901 PE=4 SV=1	[more]
A0A6J1JXQ8	4.9e-51	80.80	basic blue protein-like OS=Cucurbita maxima OX=3661 GN=LOC111489786 PE=4 SV=1	[more]

BLAST of Lsi08G005120 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0058846.1	3.8e-58	84.85	basic blue protein-like [Cucumis melo var. makuwa] >TYK23773.1 basic blue protei...	[more]
XP_004144257.3	5.0e-58	84.09	basic blue protein-like [Cucumis sativus] >KAE8647192.1 hypothetical protein Csa...	[more]
XP_038885676.1	8.5e-58	86.47	basic blue protein-like [Benincasa hispida]	[more]
XP_008441659.2	1.5e-57	84.73	PREDICTED: basic blue protein-like [Cucumis melo]	[more]
XP_023550997.1	7.0e-52	81.60	basic blue protein-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi08G005120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02850.1	1.2e-36	55.04	plantacyanin	[more]
AT1G17800.1	1.9e-18	39.39	early nodulin-like protein 22	[more]
AT3G60270.1	2.0e-17	39.17	Cupredoxin superfamily protein	[more]
AT3G17675.1	2.3e-16	38.38	Cupredoxin superfamily protein	[more]
AT5G26330.1	3.9e-16	37.70	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 47..125 e-value: 3.9E-26 score: 90.9
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 37..132 score: 36.865406
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 37..131 e-value: 1.2E-31 score: 110.8
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 37..131
None	No IPR available	PANTHER	PTHR33021:SF242	BASIC BLUE-LIKE PROTEIN	coord: 16..130
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 16..130
IPR028871	Blue (type 1) copper protein, binding site	PROSITE	PS00196	COPPER_BLUE	coord: 109..125
IPR041844	Plantacyanin	CDD	cd11013	Plantacyanin	coord: 37..131 e-value: 5.37861E-55 score: 165.22

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G005120.1	Lsi08G005120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048653	anther development
biological_process	GO:0022900	electron transport chain
biological_process	GO:0009856	pollination
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0031012	extracellular matrix
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity
molecular_function	GO:0046872	metal ion binding