Lsi08G005040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G005040
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type domain-containing protein
Location	chr08: 12960070 .. 12960369 (-)
RNA-Seq Expression	Lsi08G005040
Synteny	Lsi08G005040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGCTCAAGTCACTGAAAAAGAGCCTTGAAGGGTCGTGCTTAACGAAGAGTTGTTGTAAAGAGGAGTGCATGGTGTGCTTGGAAGGGTTTAAAGAGGAAACGAAGACAAAACATAGTGTAAGTCGGCTTCCATGTTCTCATATGTTTCATTTTTGGTGCGTTTCTCAATGGCTTAGTTTGGATTATCGTTGCCCGGTTTGCCGTTCTCCCATGCCTTGGTTTCAAGTCTTCATCCTTGATGTCGACGTTGATGTGCCTTTTCCTTTTAACGTAATTCCATTACACCTCTTACCTTGA

mRNA sequence

ATGGTGCTCAAGTCACTGAAAAAGAGCCTTGAAGGGTCGTGCTTAACGAAGAGTTGTTGTAAAGAGGAGTGCATGGTGTGCTTGGAAGGGTTTAAAGAGGAAACGAAGACAAAACATAGTGTAAGTCGGCTTCCATGTTCTCATATGTTTCATTTTTGGTGCGTTTCTCAATGGCTTAGTTTGGATTATCGTTGCCCGGTTTGCCGTTCTCCCATGCCTTGGTTTCAAGTCTTCATCCTTGATGTCGACGTTGATGTGCCTTTTCCTTTTAACGTAATTCCATTACACCTCTTACCTTGA

Coding sequence (CDS)

ATGGTGCTCAAGTCACTGAAAAAGAGCCTTGAAGGGTCGTGCTTAACGAAGAGTTGTTGTAAAGAGGAGTGCATGGTGTGCTTGGAAGGGTTTAAAGAGGAAACGAAGACAAAACATAGTGTAAGTCGGCTTCCATGTTCTCATATGTTTCATTTTTGGTGCGTTTCTCAATGGCTTAGTTTGGATTATCGTTGCCCGGTTTGCCGTTCTCCCATGCCTTGGTTTCAAGTCTTCATCCTTGATGTCGACGTTGATGTGCCTTTTCCTTTTAACGTAATTCCATTACACCTCTTACCTTGA

Protein sequence

MVLKSLKKSLEGSCLTKSCCKEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMPWFQVFILDVDVDVPFPFNVIPLHLLP

Homology

[Close]

BLAST of Lsi08G005040 vs. ExPASy Swiss-Prot
Match: Q6NRV8 (E3 ubiquitin-protein ligase arkadia-B OS=Xenopus laevis OX=8355 GN=rnf111-b PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.1e-07
Identity = 28/84 (33.33%), Postives = 42/84 (50.00%), Query Frame = 0


Query: 4   KSLKKSLEGSCLTKSCCKEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDY 63
           + L    +G   T+   +E+C +CL   +E       V RLPC H+FH  CV QWL  + 
Sbjct: 887 RKLHSKQDGEEATEEDTEEKCTICLSILEE----GEDVRRLPCMHLFHQVCVDQWLITNK 946

Query: 64  RCPVCRSPMPWFQVFILDVDVDVP 88
           +CP+CR          +D+D  +P
Sbjct: 947 KCPICR----------VDIDTQLP 956

[Close]

BLAST of Lsi08G005040 vs. ExPASy Swiss-Prot
Match: Q0V9R0 (E3 ubiquitin-protein ligase arkadia OS=Xenopus tropicalis OX=8364 GN=rnf111 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 3.1e-07
Identity = 28/84 (33.33%), Postives = 42/84 (50.00%), Query Frame = 0


Query: 4   KSLKKSLEGSCLTKSCCKEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDY 63
           + L    +G   T+   +E+C +CL   +E       V RLPC H+FH  CV QWL  + 
Sbjct: 882 RKLHSKQDGEEATEEDTEEKCTICLSILEE----GEDVRRLPCMHLFHQVCVDQWLITNK 941

Query: 64  RCPVCRSPMPWFQVFILDVDVDVP 88
           +CP+CR          +D+D  +P
Sbjct: 942 KCPICR----------VDIDTQLP 951

[Close]

BLAST of Lsi08G005040 vs. ExPASy Swiss-Prot
Match: Q8GYT9 (E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 3.1e-07
Identity = 23/47 (48.94%), Postives = 29/47 (61.70%), Query Frame = 0


Query: 23  ECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCR 70
           EC++CLE F       H V  LPC+H FH  C+ QWL L+ +CP CR
Sbjct: 234 ECLICLEEF----HIGHEVRGLPCAHNFHVECIDQWLRLNVKCPRCR 276

[Close]

BLAST of Lsi08G005040 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 7.0e-07
Identity = 26/70 (37.14%), Postives = 40/70 (57.14%), Query Frame = 0


Query: 4   KSLKKSLEGSCLTKSCCKEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDY 63
           K++ +SL  + +  S  + +C VCL  F+EE     +V  +PC H+FH  C+  WLS   
Sbjct: 68  KAVVESLPRTVIRSSKAELKCPVCLLEFEEE----ETVIEMPCHHLFHSNCILPWLSKTN 127

Query: 64  RCPVCRSPMP 74
            CP+CR  +P
Sbjct: 128 SCPLCRHELP 133

[Close]

BLAST of Lsi08G005040 vs. ExPASy Swiss-Prot
Match: Q6ZSG1 (E3 ubiquitin-protein ligase RNF165 OS=Homo sapiens OX=9606 GN=RNF165 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 9.1e-07
Identity = 21/48 (43.75%), Postives = 30/48 (62.50%), Query Frame = 0


Query: 22  EECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCR 70
           E+C +CL   ++       V RLPC H+FH  CV QWL++  +CP+CR
Sbjct: 292 EKCTICLSMLED----GEDVRRLPCMHLFHQLCVDQWLAMSKKCPICR 335

[Close]

BLAST of Lsi08G005040 vs. ExPASy TrEMBL
Match: A0A0E0F9F3 (RING-type domain-containing protein OS=Oryza meridionalis OX=40149 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.9e-08
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 0


Query: 21 KEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
          + +C VCLEGF +  K    ++R+PCSH FH  C+  WL L +RCP+CR PMP
Sbjct: 14 ESDCAVCLEGFDQGEK----LTRMPCSHCFHATCILDWLRLSHRCPLCRFPMP 62

[Close]

BLAST of Lsi08G005040 vs. ExPASy TrEMBL
Match: A0A0E0F9F4 (RING-type domain-containing protein OS=Oryza meridionalis OX=40149 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.9e-08
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 0


Query: 21  KEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           + +C VCLEGF +  K    ++R+PCSH FH  C+  WL L +RCP+CR PMP
Sbjct: 143 ESDCAVCLEGFDQGEK----LTRMPCSHCFHATCILDWLRLSHRCPLCRFPMP 191

[Close]

BLAST of Lsi08G005040 vs. ExPASy TrEMBL
Match: A0A0E0J6K4 (RING-type domain-containing protein OS=Oryza nivara OX=4536 PE=4 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 5.0e-08
Identity = 28/63 (44.44%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 11  EGSCLTKSCCKEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRS 70
           EG        + +C VC EGF +  K    ++R+PCSH FH  C+  WLSL + CP+CR 
Sbjct: 71  EGEAQVSEARESDCAVCFEGFDQGEK----LTRMPCSHCFHATCILDWLSLSHLCPLCRF 129

Query: 71  PMP 74
           PMP
Sbjct: 131 PMP 129

[Close]

BLAST of Lsi08G005040 vs. ExPASy TrEMBL
Match: A0A0D3HRB9 (RING-type domain-containing protein OS=Oryza barthii OX=65489 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.5e-07
Identity = 26/53 (49.06%), Postives = 35/53 (66.04%), Query Frame = 0


Query: 21  KEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           + +C VC EGF +  K    ++R+PCSH FH  C+  WLSL + CP+CR PMP
Sbjct: 137 ESDCAVCFEGFDQGEK----LTRMPCSHCFHATCILDWLSLSHLCPLCRFPMP 185

[Close]

BLAST of Lsi08G005040 vs. ExPASy TrEMBL
Match: A0A0P0Y6W6 (Os12g0142850 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0142850 PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 1.5e-07
Identity = 26/53 (49.06%), Postives = 35/53 (66.04%), Query Frame = 0


Query: 21 KEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
          + +C VC EGF +  K    ++R+PCSH FH  C+  WLSL + CP+CR PMP
Sbjct: 30 ESDCAVCFEGFDQGEK----LTRMPCSHCFHATCILDWLSLSHLCPLCRFPMP 78

[Close]

BLAST of Lsi08G005040 vs. NCBI nr
Match: XP_029116802.1 (uncharacterized protein LOC114912798 [Elaeis guineensis])

HSP 1 Score: 68.2 bits (165), Expect = 4.6e-08
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = 0


Query: 21  KEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           +EEC++CL  F  + K    VSR+PCSH FH WC+ +WL     CP+CRS MP
Sbjct: 186 EEECVICLMEFDAQAK----VSRMPCSHTFHSWCIIRWLETSSLCPICRSQMP 234

[Close]

BLAST of Lsi08G005040 vs. NCBI nr
Match: KAF2906628.1 (hypothetical protein DAI22_12g033800 [Oryza sativa Japonica Group] >BAT15857.1 Os12g0142850 [Oryza sativa Japonica Group])

HSP 1 Score: 65.5 bits (158), Expect = 3.0e-07
Identity = 26/53 (49.06%), Postives = 35/53 (66.04%), Query Frame = 0


Query: 21 KEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
          + +C VC EGF +  K    ++R+PCSH FH  C+  WLSL + CP+CR PMP
Sbjct: 30 ESDCAVCFEGFDQGEK----LTRMPCSHCFHATCILDWLSLSHLCPLCRFPMP 78

[Close]

BLAST of Lsi08G005040 vs. NCBI nr
Match: XP_015162731.1 (PREDICTED: E3 ubiquitin-protein ligase SDIR1-like [Solanum tuberosum])

HSP 1 Score: 64.7 bits (156), Expect = 5.1e-07
Identity = 28/53 (52.83%), Postives = 37/53 (69.81%), Query Frame = 0


Query: 21  KEECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           K++C+VCL+   EET+    V RLPCSHMFH  CV++WL   + CP+CR  MP
Sbjct: 114 KDDCLVCLDEIGEETQ----VLRLPCSHMFHGDCVTKWLENSHYCPLCRFEMP 162

[Close]

BLAST of Lsi08G005040 vs. NCBI nr
Match: PQP95452.1 (uncharacterized protein Pyn_08210 [Prunus yedoensis var. nudiflora])

HSP 1 Score: 64.7 bits (156), Expect = 5.1e-07
Identity = 25/50 (50.00%), Postives = 35/50 (70.00%), Query Frame = 0


Query: 24  CMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           C++C+E F E+    H+ SRLPCSH++H  C+ +WL   + CPVCR PMP
Sbjct: 186 CIICMENFGEDDFGGHA-SRLPCSHLYHEDCIVEWLETSHLCPVCRYPMP 234

[Close]

BLAST of Lsi08G005040 vs. NCBI nr
Match: XP_022755027.1 (NEP1-interacting protein-like 1 [Durio zibethinus])

HSP 1 Score: 64.3 bits (155), Expect = 6.7e-07
Identity = 25/52 (48.08%), Postives = 36/52 (69.23%), Query Frame = 0


Query: 22  EECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           EECM+C+E     T+    V+ +PCSH+FH  C+ +WL+  +RCP+CR PMP
Sbjct: 184 EECMICMEQLSPGTE----VTCMPCSHLFHGDCIEKWLNTSHRCPLCRFPMP 231

[Close]

BLAST of Lsi08G005040 vs. TAIR 10
Match: AT4G26580.1 (RING/U-box superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 3.4e-09
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 0


Query: 23  ECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           EC +CL  +KE    K  V +LPCSH FH  CV QWL +   CP+C+  +P
Sbjct: 288 ECCICLAKYKE----KEEVRKLPCSHRFHLKCVDQWLRIISCCPLCKQDLP 334

[Close]

BLAST of Lsi08G005040 vs. TAIR 10
Match: AT4G26580.2 (RING/U-box superfamily protein )

HSP 1 Score: 58.5 bits (140), Expect = 3.4e-09
Identity = 24/51 (47.06%), Postives = 31/51 (60.78%), Query Frame = 0


Query: 23  ECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           EC +CL  +KE    K  V +LPCSH FH  CV QWL +   CP+C+  +P
Sbjct: 288 ECCICLAKYKE----KEEVRKLPCSHRFHLKCVDQWLRIISCCPLCKQDLP 334

[Close]

BLAST of Lsi08G005040 vs. TAIR 10
Match: AT1G74620.1 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.3e-08
Identity = 21/50 (42.00%), Postives = 34/50 (68.00%), Query Frame = 0


Query: 24  CMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           C +C+E + E +     V++LPC H FH  C+++WL L++ CP+CRS +P
Sbjct: 183 CAICMEDYIEGSSI---VAKLPCDHEFHGDCINKWLQLNHMCPLCRSSIP 229

[Close]

BLAST of Lsi08G005040 vs. TAIR 10
Match: AT5G55970.1 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.3e-08
Identity = 23/51 (45.10%), Postives = 31/51 (60.78%), Query Frame = 0


Query: 23  ECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           EC +CL  +K+    K  V +LPCSH FH  CV QWL +   CP+C+  +P
Sbjct: 296 ECCICLAKYKD----KEEVRKLPCSHKFHSKCVDQWLRIISCCPLCKQDLP 342

[Close]

BLAST of Lsi08G005040 vs. TAIR 10
Match: AT5G55970.2 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.3e-08
Identity = 23/51 (45.10%), Postives = 31/51 (60.78%), Query Frame = 0


Query: 23  ECMVCLEGFKEETKTKHSVSRLPCSHMFHFWCVSQWLSLDYRCPVCRSPMP 74
           EC +CL  +K+    K  V +LPCSH FH  CV QWL +   CP+C+  +P
Sbjct: 296 ECCICLAKYKD----KEEVRKLPCSHKFHSKCVDQWLRIISCCPLCKQDLP 342

The following BLAST results are available for this feature:

BLAST of Lsi08G005040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NRV8	3.1e-07	33.33	E3 ubiquitin-protein ligase arkadia-B OS=Xenopus laevis OX=8355 GN=rnf111-b PE=2...	[more]
Q0V9R0	3.1e-07	33.33	E3 ubiquitin-protein ligase arkadia OS=Xenopus tropicalis OX=8364 GN=rnf111 PE=2...	[more]
Q8GYT9	3.1e-07	48.94	E3 ubiquitin-protein ligase SIS3 OS=Arabidopsis thaliana OX=3702 GN=SIS3 PE=2 SV...	[more]
Q6AXU4	7.0e-07	37.14	E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ...	[more]
Q6ZSG1	9.1e-07	43.75	E3 ubiquitin-protein ligase RNF165 OS=Homo sapiens OX=9606 GN=RNF165 PE=1 SV=1	[more]

BLAST of Lsi08G005040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0E0F9F3	2.9e-08	50.94	RING-type domain-containing protein OS=Oryza meridionalis OX=40149 PE=4 SV=1	[more]
A0A0E0F9F4	2.9e-08	50.94	RING-type domain-containing protein OS=Oryza meridionalis OX=40149 PE=4 SV=1	[more]
A0A0E0J6K4	5.0e-08	44.44	RING-type domain-containing protein OS=Oryza nivara OX=4536 PE=4 SV=1	[more]
A0A0D3HRB9	1.5e-07	49.06	RING-type domain-containing protein OS=Oryza barthii OX=65489 PE=4 SV=1	[more]
A0A0P0Y6W6	1.5e-07	49.06	Os12g0142850 protein OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0142850 PE...	[more]

BLAST of Lsi08G005040 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_029116802.1	4.6e-08	50.94	uncharacterized protein LOC114912798 [Elaeis guineensis]	[more]
KAF2906628.1	3.0e-07	49.06	hypothetical protein DAI22_12g033800 [Oryza sativa Japonica Group] >BAT15857.1 O...	[more]
XP_015162731.1	5.1e-07	52.83	PREDICTED: E3 ubiquitin-protein ligase SDIR1-like [Solanum tuberosum]	[more]
PQP95452.1	5.1e-07	50.00	uncharacterized protein Pyn_08210 [Prunus yedoensis var. nudiflora]	[more]
XP_022755027.1	6.7e-07	48.08	NEP1-interacting protein-like 1 [Durio zibethinus]	[more]

BLAST of Lsi08G005040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G26580.1	3.4e-09	47.06	RING/U-box superfamily protein	[more]
AT4G26580.2	3.4e-09	47.06	RING/U-box superfamily protein	[more]
AT1G74620.1	1.3e-08	42.00	RING/U-box superfamily protein	[more]
AT5G55970.1	1.3e-08	45.10	RING/U-box superfamily protein	[more]
AT5G55970.2	1.3e-08	45.10	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 24..68 e-value: 3.6E-7 score: 39.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 23..69 e-value: 2.9E-12 score: 46.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 24..69 score: 12.725326
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 22..74 e-value: 6.2E-15 score: 56.4
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 20..74

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G005040.1	Lsi08G005040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044267	cellular protein metabolic process