Lsi08G004690 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G004690
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCalcium-binding EF-hand family protein
Locationchr08: 12421901 .. 12422398 (+)
RNA-Seq ExpressionLsi08G004690
SyntenyLsi08G004690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCAATTAGCTCCAATGACTTGCACAAGATCTTCAAGAAGCTAGACAAGAATGGAGATGGCTTAATTTGCTTGCAAGAGCTCAAGTGGCTGTTAGATAGGATTGGCATCCAATTAACCATGGAAGAATTGGAGTCATTTCTAGAGAAACCGAGTCTCGACTTCGACGAGTTCTTCTTCTTTTACGAGTCGATATCGAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGATGAGGGATGTGTGAAAGATAATGATGATCATGAAGAAGATATGGAGATTGTATATATGGCTTTCAAAGTGTTTGACATGAATGGTGATGGCTACATTTCTTGCGACGAGCTCGAAAACGTGTTGGCGAGACTCGAGCTTTGGGACGCTAATAGAAACGACGACAACGTCGATTATTGTAGAAGTATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAGGAGTTTAAGAATATGATGTTGCTCACAACATAG

mRNA sequence

ATGTCTTCAATTAGCTCCAATGACTTGCACAAGATCTTCAAGAAGCTAGACAAGAATGGAGATGGCTTAATTTGCTTGCAAGAGCTCAAGTGGCTGTTAGATAGGATTGGCATCCAATTAACCATGGAAGAATTGGAGTCATTTCTAGAGAAACCGAGTCTCGACTTCGACGAGTTCTTCTTCTTTTACGAGTCGATATCGAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGATGAGGGATGTGTGAAAGATAATGATGATCATGAAGAAGATATGGAGATTGTATATATGGCTTTCAAAGTGTTTGACATGAATGGTGATGGCTACATTTCTTGCGACGAGCTCGAAAACGTGTTGGCGAGACTCGAGCTTTGGGACGCTAATAGAAACGACGACAACGTCGATTATTGTAGAAGTATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAGGAGTTTAAGAATATGATGTTGCTCACAACATAG

Coding sequence (CDS)

ATGTCTTCAATTAGCTCCAATGACTTGCACAAGATCTTCAAGAAGCTAGACAAGAATGGAGATGGCTTAATTTGCTTGCAAGAGCTCAAGTGGCTGTTAGATAGGATTGGCATCCAATTAACCATGGAAGAATTGGAGTCATTTCTAGAGAAACCGAGTCTCGACTTCGACGAGTTCTTCTTCTTTTACGAGTCGATATCGAAGCAAAACAAAGGTGAGTGCAAAGGGGGTGATGAGGGATGTGTGAAAGATAATGATGATCATGAAGAAGATATGGAGATTGTATATATGGCTTTCAAAGTGTTTGACATGAATGGTGATGGCTACATTTCTTGCGACGAGCTCGAAAACGTGTTGGCGAGACTCGAGCTTTGGGACGCTAATAGAAACGACGACAACGTCGATTATTGTAGAAGTATGATCCGAGCTTATGACACCAATTTGGATGGGAAGCTGGATTTTGAGGAGTTTAAGAATATGATGTTGCTCACAACATAG

Protein sequence

MSSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFFFFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Homology
BLAST of Lsi08G004690 vs. ExPASy Swiss-Prot
Match: Q9LPK5 (Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 PE=2 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 1.5e-30
Identity = 80/162 (49.38%), Postives = 101/162 (62.35%), Query Frame = 0

Query: 2   SSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFF 61
           S I++NDL ++FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SLD DEF 
Sbjct: 4   SFITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFL 63

Query: 62  FFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLA 121
            FY      +KG  K  D   V DND      E +  AF VFD+NGDGYIS +EL +VL 
Sbjct: 64  RFYYDAVLDSKGSKKNID--VVADND------EAIARAFNVFDVNGDGYISAEELRDVLE 123

Query: 122 RLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           RL   +  +  D    C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 124 RLGFEEEAKAWD----CGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of Lsi08G004690 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 5.9e-19
Identity = 61/165 (36.97%), Postives = 90/165 (54.55%), Query Frame = 0

Query: 3   SISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLE------KPSLDF 62
           S+++  L +IF   DKNGDG I ++EL   L R+G+   + +L+S +E         L+F
Sbjct: 24  SLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNF 83

Query: 63  DEFFFFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELE 122
           D+F   ++++     G   GG E    D     E+   +  AFKVFD NGDG+IS  EL+
Sbjct: 84  DDFSSLHKTLDDSFFGGACGGGEN-EDDPSSAAENESDLAEAFKVFDENGDGFISARELQ 143

Query: 123 NVLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
            VL +L L +       ++    MI + D N DG++DF EFKNMM
Sbjct: 144 TVLKKLGLPEGG----EMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Lsi08G004690 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 7.8e-19
Identity = 63/172 (36.63%), Postives = 88/172 (51.16%), Query Frame = 0

Query: 4   ISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEK------PSLDFD 63
           + S +L K+FK  DKNGDG I  +EL       GI +  +EL++ ++K        +D +
Sbjct: 1   MDSTELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVE 60

Query: 64  EFFFFYESI--------SKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGY 123
           EF   Y SI        + +      GG+ G   D D+   +      AF VFD NGDG+
Sbjct: 61  EFGLLYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDEGMRE------AFNVFDQNGDGF 120

Query: 124 ISCDELENVLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           I+ DEL +VL+ L L      DD    CR MI   D + DG++DF+EFK MM
Sbjct: 121 ITVDELRSVLSSLGLKHGRTADD----CRRMISMVDADGDGRVDFKEFKQMM 162

BLAST of Lsi08G004690 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 6.6e-18
Identity = 59/165 (35.76%), Postives = 92/165 (55.76%), Query Frame = 0

Query: 3   SISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFL------EKPSLDF 62
           S+++  LH++F   DKN DG I ++EL   L R+G+     +L+S +      +K  L F
Sbjct: 23  SLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRF 82

Query: 63  DEFFFFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELE 122
           D+F   ++++ +       GG+  C   +   E D+E    AF VFD +GDG+IS  EL+
Sbjct: 83  DDFAALHKTLDE----SFFGGEGSCC--DGSPESDLE---EAFNVFDEDGDGFISAVELQ 142

Query: 123 NVLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
            VL +L L +A      ++    MI + D+N DG++DF EFKNMM
Sbjct: 143 KVLKKLGLPEAG----EIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Lsi08G004690 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.6e-18
Identity = 61/174 (35.06%), Postives = 96/174 (55.17%), Query Frame = 0

Query: 3   SISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLE------KPSLDF 62
           S+++  L +IF   DKN DG+I + EL   L+ +G++  + ELES ++         L F
Sbjct: 35  SLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELESTVKSFTREGNIGLQF 94

Query: 63  DEFFFFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMA---------FKVFDMNGD 122
           ++F   ++S++  +     GG+     D DD+EEDM    ++         FKVFD +GD
Sbjct: 95  EDFISLHQSLN--DSYFAYGGE-----DEDDNEEDMRKSILSQEEADSFGGFKVFDEDGD 154

Query: 123 GYISCDELENVLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           GYIS  EL+ VL +L   + +     +D    MI + D+N DG++DF EFK+MM
Sbjct: 155 GYISARELQMVLGKLGFSEGS----EIDRVEKMIVSVDSNRDGRVDFFEFKDMM 197

BLAST of Lsi08G004690 vs. ExPASy TrEMBL
Match: A0A5D3B6U1 (Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold282G00080 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.6e-75
Identity = 144/165 (87.27%), Postives = 153/165 (92.73%), Query Frame = 0

Query: 1   MSSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFF 60
           MS ISS DLH+IFKKLDKN DGL+CLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEF 
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLA 120
           FFYESISKQNKGECKGG  GCV+  DDHE+D EIVYMAFKVFDMNGDG+ISCDELENVL 
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQ--DDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLV 120

Query: 121 RLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           RLELWDA+R++  VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 RLELWDASRSE--VDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Lsi08G004690 vs. ExPASy TrEMBL
Match: A0A1S3B3T2 (probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.6e-75
Identity = 144/165 (87.27%), Postives = 153/165 (92.73%), Query Frame = 0

Query: 1   MSSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFF 60
           MS ISS DLH+IFKKLDKN DGL+CLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEF 
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLA 120
           FFYESISKQNKGECKGG  GCV+  DDHE+D EIVYMAFKVFDMNGDG+ISCDELENVL 
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQ--DDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLV 120

Query: 121 RLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           RLELWDA+R++  VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 RLELWDASRSE--VDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Lsi08G004690 vs. ExPASy TrEMBL
Match: A0A0A0KFQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.5e-73
Identity = 141/167 (84.43%), Postives = 152/167 (91.02%), Query Frame = 0

Query: 1   MSSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFF 60
           M  ISS DLH+IFKKLDKN DGLICLQEL WLLD IGIQLTMEELESFLE+PSLDFDEF 
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGDEGCVKDNDDH--EEDMEIVYMAFKVFDMNGDGYISCDELENV 120
           FFYESISKQNKGECKGG  GCV+DND+   ++DMEIVY+AFKVFDMNGDG+ISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           L +LELWDA+R+D  VDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSD--VDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of Lsi08G004690 vs. ExPASy TrEMBL
Match: A0A6J1K1Y7 (probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC111489388 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.5e-65
Identity = 130/161 (80.75%), Postives = 145/161 (90.06%), Query Frame = 0

Query: 4   ISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFFFFY 63
           ISSNDL +IF +LDKNGDGLIC+QELKWLLD IGIQLTMEELESFLEKPSLDFDEF  FY
Sbjct: 5   ISSNDLLRIFNRLDKNGDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLIFY 64

Query: 64  ESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLARLE 123
           ES+SKQNKGECKG   G V +++D +ED+EIVYMAFKVFD+NGDG+ISC+ELENVL+RLE
Sbjct: 65  ESMSKQNKGECKG---GLVDEHEDEDEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLE 124

Query: 124 LWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           LWDANR++  VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 125 LWDANRSE--VD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Lsi08G004690 vs. ExPASy TrEMBL
Match: A0A6J1FLU5 (probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC111445399 PE=4 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.6e-65
Identity = 131/161 (81.37%), Postives = 144/161 (89.44%), Query Frame = 0

Query: 4   ISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFFFFY 63
           ISSNDL +IF +LDKN DGLIC+QELKWLLD IGIQLTMEELESFLEKPSLDFDEF FFY
Sbjct: 5   ISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFFY 64

Query: 64  ESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLARLE 123
           ESISKQNKGECKG   G V ++ D EED+EIVYMAFKVFD+NGDG+ISC+ELENVL+RLE
Sbjct: 65  ESISKQNKGECKG---GLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLE 124

Query: 124 LWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           LWDAN+++  VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 125 LWDANKSE--VD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Lsi08G004690 vs. NCBI nr
Match: XP_038885742.1 (probable calcium-binding protein CML44 [Benincasa hispida])

HSP 1 Score: 295.4 bits (755), Expect = 3.0e-76
Identity = 147/165 (89.09%), Postives = 155/165 (93.94%), Query Frame = 0

Query: 1   MSSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFF 60
           MSSIS NDLH+IF KLDKNGDGLICLQELKWL DRIGIQLTMEELESFLEKPSLDF+EF 
Sbjct: 11  MSSISFNDLHRIFNKLDKNGDGLICLQELKWLFDRIGIQLTMEELESFLEKPSLDFNEFL 70

Query: 61  FFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLA 120
           FFYESISKQNKGECKGG  GC++  DDHEE+MEIVYMAFKVFDMNGDG+ISCDELENVL+
Sbjct: 71  FFYESISKQNKGECKGG-VGCLQ--DDHEEEMEIVYMAFKVFDMNGDGFISCDELENVLS 130

Query: 121 RLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           RLELWDANRND  +DYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 131 RLELWDANRND--IDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 170

BLAST of Lsi08G004690 vs. NCBI nr
Match: XP_008441608.1 (PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 putative calcium-binding protein CML44 [Cucumis melo var. makuwa])

HSP 1 Score: 290.4 bits (742), Expect = 9.6e-75
Identity = 144/165 (87.27%), Postives = 153/165 (92.73%), Query Frame = 0

Query: 1   MSSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFF 60
           MS ISS DLH+IFKKLDKN DGL+CLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEF 
Sbjct: 1   MSPISSKDLHRIFKKLDKNCDGLLCLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLA 120
           FFYESISKQNKGECKGG  GCV+  DDHE+D EIVYMAFKVFDMNGDG+ISCDELENVL 
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQ--DDHEDDTEIVYMAFKVFDMNGDGFISCDELENVLV 120

Query: 121 RLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           RLELWDA+R++  VDYCRSMI+AYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 RLELWDASRSE--VDYCRSMIQAYDTNLDGKLDFEEFKNMMLLTT 161

BLAST of Lsi08G004690 vs. NCBI nr
Match: XP_004149438.1 (probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetical protein Csa_018262 [Cucumis sativus])

HSP 1 Score: 284.6 bits (727), Expect = 5.3e-73
Identity = 141/167 (84.43%), Postives = 152/167 (91.02%), Query Frame = 0

Query: 1   MSSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFF 60
           M  ISS DLH+IFKKLDKN DGLICLQEL WLLD IGIQLTMEELESFLE+PSLDFDEF 
Sbjct: 1   MPPISSKDLHRIFKKLDKNCDGLICLQELNWLLDSIGIQLTMEELESFLERPSLDFDEFL 60

Query: 61  FFYESISKQNKGECKGGDEGCVKDNDDH--EEDMEIVYMAFKVFDMNGDGYISCDELENV 120
           FFYESISKQNKGECKGG  GCV+DND+   ++DMEIVY+AFKVFDMNGDG+ISCDELENV
Sbjct: 61  FFYESISKQNKGECKGGVAGCVQDNDNDQGQDDMEIVYLAFKVFDMNGDGFISCDELENV 120

Query: 121 LARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 166
           L +LELWDA+R+D  VDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT
Sbjct: 121 LVKLELWDASRSD--VDYCRSMIRAYDTNLDGKLDFEEFKNMMLLTT 165

BLAST of Lsi08G004690 vs. NCBI nr
Match: KAG6578937.1 (putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 259.6 bits (662), Expect = 1.8e-65
Identity = 132/161 (81.99%), Postives = 144/161 (89.44%), Query Frame = 0

Query: 4   ISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFFFFY 63
           ISSNDL +IF +LDKN DGLIC+QELKWLLD IGIQLTMEELESFLEKPSLDFDEF FFY
Sbjct: 5   ISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFFY 64

Query: 64  ESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLARLE 123
           ESISKQNKGECKG   G V ++ D EED+EIVYMAFKVFD+NGDG+ISC+ELENVL+RLE
Sbjct: 65  ESISKQNKGECKG---GLVYEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLE 124

Query: 124 LWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           LWDAN+N+  VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 125 LWDANKNE--VD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Lsi08G004690 vs. NCBI nr
Match: XP_023551499.1 (probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 259.2 bits (661), Expect = 2.4e-65
Identity = 132/161 (81.99%), Postives = 144/161 (89.44%), Query Frame = 0

Query: 4   ISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEKPSLDFDEFFFFY 63
           ISSNDL +IF +LDKN DGLIC+QELKWLLD IGIQLTMEELESFLEKPSLDFDEF FFY
Sbjct: 5   ISSNDLLRIFNRLDKNKDGLICVQELKWLLDTIGIQLTMEELESFLEKPSLDFDEFLFFY 64

Query: 64  ESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLARLE 123
           ESISKQNKGECKG   G V ++ D EED+EIVYMAFKVFD+NGDG+ISC+ELENVL+RLE
Sbjct: 65  ESISKQNKGECKG---GLVDEHGDEEEDVEIVYMAFKVFDVNGDGFISCEELENVLSRLE 124

Query: 124 LWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMMLLT 165
           LWDANR++  VD CRSMIRAYDTNLDGKLDFEEF NMML T
Sbjct: 125 LWDANRSE--VD-CRSMIRAYDTNLDGKLDFEEFMNMMLFT 159

BLAST of Lsi08G004690 vs. TAIR 10
Match: AT1G21550.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.0 bits (336), Expect = 1.1e-31
Identity = 80/162 (49.38%), Postives = 101/162 (62.35%), Query Frame = 0

Query: 2   SSISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIG-IQLTMEELESFLEKPSLDFDEFF 61
           S I++NDL ++FK LDKN DGL+ L EL W+LD++G  + T +ELE  + K SLD DEF 
Sbjct: 4   SFITTNDLRRMFKTLDKNQDGLVTLDELLWILDKLGWAEHTPDELELIVGKQSLDLDEFL 63

Query: 62  FFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLA 121
            FY      +KG  K  D   V DND      E +  AF VFD+NGDGYIS +EL +VL 
Sbjct: 64  RFYYDAVLDSKGSKKNID--VVADND------EAIARAFNVFDVNGDGYISAEELRDVLE 123

Query: 122 RLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMML 163
           RL   +  +  D    C  MIR +D NLDG +DFEEFKNM+L
Sbjct: 124 RLGFEEEAKAWD----CGRMIRVHDKNLDGFVDFEEFKNMIL 153

BLAST of Lsi08G004690 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 95.5 bits (236), Expect = 4.2e-20
Identity = 61/165 (36.97%), Postives = 90/165 (54.55%), Query Frame = 0

Query: 3   SISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLE------KPSLDF 62
           S+++  L +IF   DKNGDG I ++EL   L R+G+   + +L+S +E         L+F
Sbjct: 24  SLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKSTVESYIQPGNTGLNF 83

Query: 63  DEFFFFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELE 122
           D+F   ++++     G   GG E    D     E+   +  AFKVFD NGDG+IS  EL+
Sbjct: 84  DDFSSLHKTLDDSFFGGACGGGEN-EDDPSSAAENESDLAEAFKVFDENGDGFISARELQ 143

Query: 123 NVLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
            VL +L L +       ++    MI + D N DG++DF EFKNMM
Sbjct: 144 TVLKKLGLPEGG----EMERVEKMIVSVDRNQDGRVDFFEFKNMM 183

BLAST of Lsi08G004690 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 92.0 bits (227), Expect = 4.7e-19
Identity = 59/165 (35.76%), Postives = 92/165 (55.76%), Query Frame = 0

Query: 3   SISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFL------EKPSLDF 62
           S+++  LH++F   DKN DG I ++EL   L R+G+     +L+S +      +K  L F
Sbjct: 23  SLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKSTVDSFIKPDKTGLRF 82

Query: 63  DEFFFFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELE 122
           D+F   ++++ +       GG+  C   +   E D+E    AF VFD +GDG+IS  EL+
Sbjct: 83  DDFAALHKTLDE----SFFGGEGSCC--DGSPESDLE---EAFNVFDEDGDGFISAVELQ 142

Query: 123 NVLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
            VL +L L +A      ++    MI + D+N DG++DF EFKNMM
Sbjct: 143 KVLKKLGLPEAG----EIEQVEKMIVSVDSNHDGRVDFFEFKNMM 174

BLAST of Lsi08G004690 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 58/160 (36.25%), Postives = 87/160 (54.37%), Query Frame = 0

Query: 8   DLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEK------PSLDFDEFFF 67
           +L ++F+  DKNGDG I  +EL   L  +GI +  +EL   +EK        +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 68  FYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELENVLAR 127
            Y++I               + + D+ EEDM+    AF VFD NGDG+I+ DEL+ VL+ 
Sbjct: 65  LYKTI---------------MDEEDEEEEDMK---EAFNVFDQNGDGFITVDELKAVLSS 124

Query: 128 LELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           L L      DD    C+ MI+  D + DG+++++EF+ MM
Sbjct: 125 LGLKQGKTLDD----CKKMIKKVDVDGDGRVNYKEFRQMM 142

BLAST of Lsi08G004690 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 86.3 bits (212), Expect = 2.6e-17
Identity = 55/164 (33.54%), Postives = 88/164 (53.66%), Query Frame = 0

Query: 4   ISSNDLHKIFKKLDKNGDGLICLQELKWLLDRIGIQLTMEELESFLEK------PSLDFD 63
           I  ++L ++F+  DKNGDG I  +EL   L+ +GI +  ++L   + K        +D D
Sbjct: 61  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 120

Query: 64  EFFFFYESISKQNKGECKGGDEGCVKDNDDHEEDMEIVYMAFKVFDMNGDGYISCDELEN 123
           EF   Y SI  ++             D +  EEDM+    AF VFD +GDG+I+ +EL++
Sbjct: 121 EFESLYSSIVDEHH-----------NDGETEEEDMK---DAFNVFDQDGDGFITVEELKS 180

Query: 124 VLARLELWDANRNDDNVDYCRSMIRAYDTNLDGKLDFEEFKNMM 162
           V+A L L    +    +D C+ MI   D + DG+++++EF  MM
Sbjct: 181 VMASLGL----KQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMM 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LPK51.5e-3049.38Probable calcium-binding protein CML44 OS=Arabidopsis thaliana OX=3702 GN=CML44 ... [more]
Q9SVG95.9e-1936.97Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
Q2QVI17.8e-1936.63Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9FI196.6e-1835.76Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ... [more]
P431878.6e-1835.06Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3B6U14.6e-7587.27Putative calcium-binding protein CML44 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B3T24.6e-7587.27probable calcium-binding protein CML44 OS=Cucumis melo OX=3656 GN=LOC103485689 P... [more]
A0A0A0KFQ62.5e-7384.43Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G376250 PE=4 SV=1[more]
A0A6J1K1Y71.5e-6580.75probable calcium-binding protein CML44 OS=Cucurbita maxima OX=3661 GN=LOC1114893... [more]
A0A6J1FLU52.6e-6581.37probable calcium-binding protein CML44 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
XP_038885742.13.0e-7689.09probable calcium-binding protein CML44 [Benincasa hispida][more]
XP_008441608.19.6e-7587.27PREDICTED: probable calcium-binding protein CML44 [Cucumis melo] >TYJ95710.1 put... [more]
XP_004149438.15.3e-7384.43probable calcium-binding protein CML44 [Cucumis sativus] >KGN47684.1 hypothetica... [more]
KAG6578937.11.8e-6581.99putative calcium-binding protein CML44, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023551499.12.4e-6581.99probable calcium-binding protein CML44 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G21550.11.1e-3149.38Calcium-binding EF-hand family protein [more]
AT4G20780.14.2e-2036.97calmodulin like 42 [more]
AT5G44460.14.7e-1935.76calmodulin like 43 [more]
AT1G05990.11.0e-1836.25EF hand calcium-binding protein family [more]
AT2G43290.12.6e-1733.54Calcium-binding EF-hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 94..122
e-value: 0.0097
score: 25.1
coord: 136..164
e-value: 0.0051
score: 26.0
coord: 8..36
e-value: 6.6E-5
score: 32.3
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 8..33
e-value: 2.4E-6
score: 26.7
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 96..161
e-value: 9.7E-11
score: 42.0
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 132..165
score: 13.676544
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 4..39
score: 13.258112
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 90..125
score: 12.923368
IPR002048EF-hand domainCDDcd00051EFhcoord: 98..161
e-value: 7.35371E-11
score: 52.9353
IPR002048EF-hand domainCDDcd00051EFhcoord: 8..51
e-value: 1.85288E-7
score: 44.0757
NoneNo IPR availableGENE3D1.10.238.10coord: 67..161
e-value: 2.1E-18
score: 68.4
NoneNo IPR availableGENE3D1.10.238.10coord: 5..66
e-value: 4.8E-10
score: 41.4
NoneNo IPR availablePANTHERPTHR10891:SF788CALCIUM-BINDING PROTEIN CML44-RELATEDcoord: 1..164
NoneNo IPR availablePANTHERPTHR10891EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEINcoord: 1..164
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 17..29
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 103..115
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 7..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G004690.1Lsi08G004690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding