Lsi08G004570 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G004570 vs. ExPASy Swiss-Prot
Match: Q8KEQ0 (Protein TolB homolog OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=CT0636 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 9.5e-21
Identity = 77/254 (30.31%), Postives = 119/254 (46.85%), Query Frame = 0

Query: 413 PSFSPVGDRVAYVDF----PGLYVINRDGSNKREVFS-GAAFSTAWDPVREGVVYTSAGP 472
           P+ SP G  +A+ D+    P LY+ N     K  V   G   S AW P    +V T    
Sbjct: 208 PAVSPDGTYIAWTDYTSGKPNLYIKNIATGAKVSVNKHGVCISPAWRPGTNTLVTT---- 267

Query: 473 DFAAVSTIVDIISVNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIM 532
              +     D+  +  D   +  ++LT  G  +  P+ SPDG  + F S + G   ++I 
Sbjct: 268 --LSYEGDQDLYLIRADGTVE--RRLTKGGGIDVSPTFSPDGSKMAFVSTRQGGPQIFIQ 327

Query: 533 DAVDGESKGLRPLTEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRK 592
           D   G+   +R LT     +T  SWSP+GD I +SS +    SG  ++F I+ +G+GL +
Sbjct: 328 DMNSGQ---VRRLTYSGIYNTQPSWSPNGDKILYSSMQK---SGEINIFSINVDGSGLLQ 387

Query: 593 LFQSGSAGRANHPNWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQR 652
           L  +  +G   HP+WSPDG  +VF++   G                 +Y++  DGSN + 
Sbjct: 388 L--TSGSGNNEHPSWSPDGSMIVFSSTRDG--------------RRRLYVMNADGSNQRP 431

Query: 653 LTHNSYEDGTPTWS 662
           L +   E   P+WS
Sbjct: 448 LLNMQGEQQQPSWS 431

BLAST of Lsi08G004570 vs. ExPASy Swiss-Prot
Match: Q1GE19 (Tol-Pal system protein TolB OS=Ruegeria sp. (strain TM1040) OX=292414 GN=tolB PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 4.0e-19
Identity = 78/259 (30.12%), Postives = 127/259 (49.03%), Query Frame = 0

Query: 413 PSFSPVGDRVAYVD----FPGLYVINRDGSNKREVFSG---AAFSTAWDPVREGVVYT-S 472
           P FSP GDRV Y      FP ++V++     +R + +G    +F+  + P  + +VY+ S
Sbjct: 212 PRFSPTGDRVLYTSYESGFPQIHVLDVGRVQRRVLSAGNGSMSFAPRFAPNGQTIVYSQS 271

Query: 473 AGPDFAAVSTIVDIISVNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNL 532
            G +        D+ S++++    N ++LT+       PS SPDG  IVF S ++G   L
Sbjct: 272 QGGN-------TDLFSMDINSG--NPQRLTSAPSIETAPSFSPDGSQIVFESDRSGTSQL 331

Query: 533 YIMDAVDGESKGLRPLTEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTG 592
           Y+M A  GE+K    ++ GQ       WSP GD+IAF+       +G F + ++  +G+ 
Sbjct: 332 YVMSASGGEAK---RISFGQGRYGTPVWSPRGDYIAFTKQ----NAGRFHIGVMRTDGSE 391

Query: 593 LRKLFQSGSAGRANHPNWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSN 652
            R L  S        P WSP+G+ ++FT +  G S +             +Y + + G N
Sbjct: 392 ERLLTAS---FLDEGPTWSPNGRVIMFTRETQGASGQ-----------ARLYSVDISGRN 438

Query: 653 LQRL-THNSYEDGTPTWSP 663
           L+ + T +   D  P+WSP
Sbjct: 452 LKPVKTPDGASD--PSWSP 438

BLAST of Lsi08G004570 vs. ExPASy Swiss-Prot
Match: Q2LRP7 (Tol-Pal system protein TolB OS=Syntrophus aciditrophicus (strain SB) OX=56780 GN=tolB PE=3 SV=2)

HSP 1 Score: 97.4 bits (241), Expect = 6.8e-19
Identity = 82/288 (28.47%), Postives = 131/288 (45.49%), Query Frame = 0

Query: 387 KSNPLFFENVRSPVSNLSLFRIAGS-----FPSFSPVGDRVAYVDF----PGLYVINRDG 446
           +S+ L      S +    L R+AGS      P +SP G  +A+  +    P ++V++  G
Sbjct: 190 RSSSLHTVGFGSQIDGRDLARVAGSPSLILSPRWSPDGRYLAFSSYRDGKPDIFVVSPSG 249

Query: 447 SNKREV--FSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIISVNVDDDEKNFKKLTT 506
           S  +++  F G     AW P    ++ T    D      ++D+ S  V       ++LT 
Sbjct: 250 SGLKKIVSFQGLNLPGAWSPDGRSLLLT-LSKDGNEEIYVMDVRSGQV-------RRLTR 309

Query: 507 NGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPLTEGQWTDTMCSWSPD 566
           N   +  P  SPDG+ I F S  +G   +Y+M+A  G+   +R LT     +T  +WSP 
Sbjct: 310 NSAIDVSPVWSPDGRRIAFVSNISGSPQIYVMNADGGD---VRRLTYSGNYNTTPAWSPR 369

Query: 567 GDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHPNWSPDGKFLVFTTDN 626
           G  IA+       GSG + +F I  +G  +R+L  +  AG    P+WSPDG+FL F+  +
Sbjct: 370 GGKIAYEG---KVGSG-YQIFSIDEDGGNVRQL--TSGAGDHEFPSWSPDGRFLTFSLRS 429

Query: 627 AGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTWSPR 664
            G S               I I+  +   ++ L  ++     P WSPR
Sbjct: 430 GGRS--------------RINILNANTLEVRTLYESTDRCLGPAWSPR 446

BLAST of Lsi08G004570 vs. ExPASy Swiss-Prot
Match: Q28TS0 (Tol-Pal system protein TolB OS=Jannaschia sp. (strain CCS1) OX=290400 GN=tolB PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 4.4e-18
Identity = 81/299 (27.09%), Postives = 131/299 (43.81%), Query Frame = 0

Query: 372 THIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGDRVAYVD----F 431
           T + +    G  N R       +   + V  L+  R     P FSP GDR+ Y      F
Sbjct: 174 TRVAFIAEEGPKNARLKRLAIMDYDGANVQFLTDSRSIVLAPRFSPQGDRILYTSYESGF 233

Query: 432 PGLYVINRDGSNKREVFSGAA----FSTAWDPVREGVVYTSAGPDFAAVSTIVDIISVNV 491
           P +Y+++     +R + +  A    FS  + P  + VV++          +  DI S+++
Sbjct: 234 PQVYIMDVATVQRRPLEAIPAETMTFSPRFSPSGQSVVFSLVD------GSNTDIYSLDL 293

Query: 492 DDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPLTEG 551
               +  ++LT        PS SPDG  IVF S ++G + +YIM A  GE++ +   T  
Sbjct: 294 ATGTR--RQLTQAPSIETAPSFSPDGSQIVFESDRSGNQQIYIMPADGGEARRVSAGTGR 353

Query: 552 QWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHPNWS 611
             T     WSP GD+IAF+       +G F + ++  +G+  R L    S+     P W+
Sbjct: 354 YGTPV---WSPRGDYIAFTKQE----NGRFHIGVMRTDGSDERLL---TSSFLDEGPTWA 413

Query: 612 PDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTWSP 663
           P+G+ ++FT +  G    P            +Y + + G NLQR+         P WSP
Sbjct: 414 PNGRVIMFTRETPGDDGAP-----------AVYSVDISGRNLQRVATPGMA-SDPAWSP 442

BLAST of Lsi08G004570 vs. ExPASy Swiss-Prot
Match: Q3A097 (Tol-Pal system protein TolB OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=tolB PE=3 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 5.8e-18
Identity = 72/258 (27.91%), Postives = 115/258 (44.57%), Query Frame = 0

Query: 413 PSFSPVGDRVAYVDF----PGLYVIN-RDGSNKREVF-SGAAFSTAWDPVREGVVYT--- 472
           P FSP G  V Y  +    P LY      G   R  F  G   +  + P    +  T   
Sbjct: 204 PDFSPNGREVIYTSYRRGNPDLYRKQLSTGQEARLAFKKGLNIAARFRPDGREIALTQSA 263

Query: 473 SAGPDFAAVSTIVDIISVNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKN 532
           +  P+   + T           D    ++LT++   +  PS SP G  + F S + G  +
Sbjct: 264 TGNPELMLLGT-----------DGSRRRRLTSHWGIDVDPSWSPAGDRLAFVSDRQGNPH 323

Query: 533 LYIMDAVDGESKGLRPLTEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGT 592
           +++MD + G++   R    G++  T  +WSPDG  IAF+        G FD++ + P+GT
Sbjct: 324 IFVMDLLGGQT--ARLTANGKYNATP-AWSPDGKRIAFT----RLEKGVFDIYTVRPDGT 383

Query: 593 GLRKLFQSGSAGRANHPNWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGS 652
             R+L  +   G   HP WSPD +FLV+++D  G                 IYI++ DG+
Sbjct: 384 DERRL--TFGPGNKEHPRWSPDSRFLVYSSDQDGRKG--------------IYIMRADGT 427

Query: 653 NLQRLTHNSYEDGTPTWS 662
            ++R++    +   P WS
Sbjct: 444 GIRRISAGGGQCQHPAWS 427

BLAST of Lsi08G004570 vs. ExPASy TrEMBL
Match: A0A5D3CCB6 (DPP6 N-terminal domain-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold314G00650 PE=4 SV=1)

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 613/707 (86.70%), Postives = 651/707 (92.08%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MP LLL FLL FSSLL PS AA  G     G+SIVFTTLGRS YAFDI+TLP D N+ PS
Sbjct: 50  MPSLLLLFLLHFSSLLPPSYAAASG-----GHSIVFTTLGRSFYAFDIYTLPADANNNPS 109

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
           + DE  ITDGQSVNFNGYFPSSSS  SLISLLTNQS SF PDLELVYVTER+GIS+IFYD
Sbjct: 110 SADEILITDGQSVNFNGYFPSSSSFSSLISLLTNQSHSFTPDLELVYVTERNGISRIFYD 169

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           A+FGG G+S RRRS+LEIPHRLQIPLLD+EQ+ EVRVS KDRPSLSGDYL+YVSTHEDP 
Sbjct: 170 AVFGGTGVSARRRSELEIPHRLQIPLLDEEQKNEVRVSFKDRPSLSGDYLVYVSTHEDPR 229

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSR+LKSG+TRRLTPYGIADFSPS+SPSGIWTAVASYGEKGWAGEVEELST
Sbjct: 230 EFRTSWAAVYSRNLKSGVTRRLTPYGIADFSPSVSPSGIWTAVASYGEKGWAGEVEELST 289

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           DLYIFLTRDGS RVKVVEHGGWPCWAD+STLYFHRRGDDQWLSIY+AILPSHGEI  DSV
Sbjct: 290 DLYIFLTRDGSRRVKVVEHGGWPCWADESTLYFHRRGDDQWLSIYRAILPSHGEIFLDSV 349

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFN+VTGEFK+LT+ VSPN+
Sbjct: 350 IIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNIVTGEFKKLTETVSPNT 409

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPF+SADGT IGYHKCRGD NGRKSN LFFENVRSPVSNLSLFRIA SFPSFSP GD
Sbjct: 410 HHFNPFVSADGTRIGYHKCRGDGNGRKSNLLFFENVRSPVSNLSLFRIAVSFPSFSPAGD 469

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+AY +FPGLYVINRDGSN+REVFSGAAFSTAWDPVR+GVVYTSAGPDFA VS+ VDIIS
Sbjct: 470 RIAYANFPGLYVINRDGSNQREVFSGAAFSTAWDPVRKGVVYTSAGPDFAPVSSQVDIIS 529

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVD++E+N KKLTTNG NNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLR L
Sbjct: 530 VNVDEEERNIKKLTTNGENNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRRL 589

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEG WTDTMCSWSPDGDWIAFSSDR+NPG+GSFDLFLIHPNGTGLRKLFQSG  GRANHP
Sbjct: 590 TEGLWTDTMCSWSPDGDWIAFSSDRENPGAGSFDLFLIHPNGTGLRKLFQSGLGGRANHP 649

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NW PDGK LVFTTDNAGISAEP+SNPHHYQPYGEIY IKLDGS+LQRLTHNSYEDGTPTW
Sbjct: 650 NWRPDGKALVFTTDNAGISAEPVSNPHHYQPYGEIYTIKLDGSDLQRLTHNSYEDGTPTW 709

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYI+P+NVE LYDVEPCGFEDCHWLNQ  +A NDIRP+LTGPRCS
Sbjct: 710 SPRYINPVNVENLYDVEPCGFEDCHWLNQNNRAHNDIRPVLTGPRCS 751

BLAST of Lsi08G004570 vs. ExPASy TrEMBL
Match: A0A1S3CT97 (uncharacterized protein LOC103504088 OS=Cucumis melo OX=3656 GN=LOC103504088 PE=4 SV=1)

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 613/707 (86.70%), Postives = 651/707 (92.08%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MP LLL FLL FSSLL PS AA  G     G+SIVFTTLGRS YAFDI+TLP D N+ PS
Sbjct: 1   MPSLLLLFLLHFSSLLPPSYAAASG-----GHSIVFTTLGRSFYAFDIYTLPADANNNPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
           + DE  ITDGQSVNFNGYFPSSSS  SLISLLTNQS SF PDLELVYVTER+GIS+IFYD
Sbjct: 61  SADEILITDGQSVNFNGYFPSSSSFSSLISLLTNQSHSFTPDLELVYVTERNGISRIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           A+FGG G+S RRRS+LEIPHRLQIPLLD+EQ+ EVRVS KDRPSLSGDYL+YVSTHEDP 
Sbjct: 121 AVFGGTGVSARRRSELEIPHRLQIPLLDEEQKNEVRVSFKDRPSLSGDYLVYVSTHEDPR 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSR+LKSG+TRRLTPYGIADFSPS+SPSGIWTAVASYGEKGWAGEVEELST
Sbjct: 181 EFRTSWAAVYSRNLKSGVTRRLTPYGIADFSPSVSPSGIWTAVASYGEKGWAGEVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           DLYIFLTRDGS RVKVVEHGGWPCWAD+STLYFHRRGDDQWLSIY+AILPSHGEI  DSV
Sbjct: 241 DLYIFLTRDGSRRVKVVEHGGWPCWADESTLYFHRRGDDQWLSIYRAILPSHGEIFLDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFN+VTGEFK+LT+ VSPN+
Sbjct: 301 IIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNIVTGEFKKLTETVSPNT 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPF+SADGT IGYHKCRGD NGRKSN LFFENVRSPVSNLSLFRIA SFPSFSP GD
Sbjct: 361 HHFNPFVSADGTRIGYHKCRGDGNGRKSNLLFFENVRSPVSNLSLFRIAVSFPSFSPAGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+AY +FPGLYVINRDGSN+REVFSGAAFSTAWDPVR+GVVYTSAGPDFA VS+ VDIIS
Sbjct: 421 RIAYANFPGLYVINRDGSNQREVFSGAAFSTAWDPVRKGVVYTSAGPDFAPVSSQVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVD++E+N KKLTTNG NNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLR L
Sbjct: 481 VNVDEEERNIKKLTTNGENNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRRL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEG WTDTMCSWSPDGDWIAFSSDR+NPG+GSFDLFLIHPNGTGLRKLFQSG  GRANHP
Sbjct: 541 TEGLWTDTMCSWSPDGDWIAFSSDRENPGAGSFDLFLIHPNGTGLRKLFQSGLGGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NW PDGK LVFTTDNAGISAEP+SNPHHYQPYGEIY IKLDGS+LQRLTHNSYEDGTPTW
Sbjct: 601 NWRPDGKALVFTTDNAGISAEPVSNPHHYQPYGEIYTIKLDGSDLQRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYI+P+NVE LYDVEPCGFEDCHWLNQ  +A NDIRP+LTGPRCS
Sbjct: 661 SPRYINPVNVENLYDVEPCGFEDCHWLNQNNRAHNDIRPVLTGPRCS 702

BLAST of Lsi08G004570 vs. ExPASy TrEMBL
Match: A0A6J1FF50 (uncharacterized protein LOC111444913 OS=Cucurbita moschata OX=3662 GN=LOC111444913 PE=4 SV=1)

HSP 1 Score: 1277.3 bits (3304), Expect = 0.0e+00
Identity = 610/707 (86.28%), Postives = 649/707 (91.80%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MPL+ LCFLLQFSSLL PSSAA  G       SIVFTTLGRS YAFD+FTLP D N  PS
Sbjct: 1   MPLVFLCFLLQFSSLLPPSSAAASG-----SGSIVFTTLGRSVYAFDVFTLPADENRAPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
             DET ITDG+SVNFNGYFPSSSSS S++SLLTNQSQS RPDLELVYVTER+GIS+IFYD
Sbjct: 61  AADETLITDGESVNFNGYFPSSSSSSSILSLLTNQSQSLRPDLELVYVTERNGISRIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           AIFGG G S RRRS LEIPHRLQIPL+DDEQ+ EVRVS KDRP+LSGDYL+YVSTHEDPG
Sbjct: 121 AIFGGTGFSARRRSALEIPHRLQIPLVDDEQKNEVRVSFKDRPNLSGDYLVYVSTHEDPG 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSRSL+SG TRRLTPYGIADFSPS+SPSG+WTAVASYGEKGWAGEVEELST
Sbjct: 181 ESRTSWAAVYSRSLRSGTTRRLTPYGIADFSPSVSPSGVWTAVASYGEKGWAGEVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           D+YIFLTRDG+ RVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIY+AILPS GEIS DSV
Sbjct: 241 DIYIFLTRDGTRRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYRAILPSDGEISTDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS
Sbjct: 301 IIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPFISADGT IGYHKCRGD NGRKSN LF ENVRSPVSNLSLFRI GSFPSFSP GD
Sbjct: 361 HHFNPFISADGTRIGYHKCRGDGNGRKSNTLFLENVRSPVSNLSLFRIDGSFPSFSPAGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+A+V+FPGLYVINRDGSN+++V+ GAAFSTAWDPVREGVVYTSAGPDFA +S+ VDIIS
Sbjct: 421 RIAFVNFPGLYVINRDGSNQQKVYPGAAFSTAWDPVREGVVYTSAGPDFAPLSSQVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVDDDE+  K+LT NG NNAFPSPSPDGKWIVFRSG++GYKNLYIMDAVDGE+K L  L
Sbjct: 481 VNVDDDERTIKRLTKNGENNAFPSPSPDGKWIVFRSGRSGYKNLYIMDAVDGENKALHRL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEGQWTDTMCSWSPDGDWIAF+SDR NPG+GSFDLFLIHPNGTGLRKLFQSGSAGRANHP
Sbjct: 541 TEGQWTDTMCSWSPDGDWIAFASDRHNPGAGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NWSPDGK LVFTTDNAGISAEPISNPHHYQPYGEIY IK+DGS+LQRLTHNSYEDGTPTW
Sbjct: 601 NWSPDGKVLVFTTDNAGISAEPISNPHHYQPYGEIYTIKMDGSDLQRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYISP+NV+  YDVEPCGFEDCHWLNQK KA N+I+P LTGPRCS
Sbjct: 661 SPRYISPVNVQNSYDVEPCGFEDCHWLNQKNKAQNEIKPDLTGPRCS 702

BLAST of Lsi08G004570 vs. ExPASy TrEMBL
Match: A0A6J1K3I0 (uncharacterized protein LOC111489739 OS=Cucurbita maxima OX=3661 GN=LOC111489739 PE=4 SV=1)

HSP 1 Score: 1276.5 bits (3302), Expect = 0.0e+00
Identity = 610/707 (86.28%), Postives = 648/707 (91.65%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MPL+ LCFLLQFSSLL PS  A  GGD     SIVFTTLGRS YAFD+FTLP D N  PS
Sbjct: 1   MPLVFLCFLLQFSSLLPPSLVAASGGD-----SIVFTTLGRSVYAFDVFTLPADENRAPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
             DET ITDG+SVNFNGYFPSSSSS S+ISLLTNQSQS RPDLELVYVTER+GIS+IFYD
Sbjct: 61  AADETLITDGESVNFNGYFPSSSSSSSIISLLTNQSQSLRPDLELVYVTERNGISRIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           AI+GG G S RRRS LEIPHRLQIPLLDDEQ+ EVRVS KDRPSLSGDYL+YVSTHEDPG
Sbjct: 121 AIYGGTGFSTRRRSALEIPHRLQIPLLDDEQKNEVRVSFKDRPSLSGDYLVYVSTHEDPG 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSRSL+SG TRRLTPYGIADFSPS+SPSG+WTAVASYGEKGWAGEVEELST
Sbjct: 181 ESRTSWAAVYSRSLRSGTTRRLTPYGIADFSPSVSPSGVWTAVASYGEKGWAGEVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           D+YIFLTRDG+ RVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIY+AILPS GEIS DSV
Sbjct: 241 DIYIFLTRDGTRRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYRAILPSDGEISTDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVA+RRPDSSFRHIELFNLVTGEFKELTK VSPNS
Sbjct: 301 IIERLTPPGLHVFTPATSPANKNLIAVASRRPDSSFRHIELFNLVTGEFKELTKVVSPNS 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPFISADGT IGYHKCRGD NGRKSN LF ENVRSPVSNLSLFRI GSFPSFSP GD
Sbjct: 361 HHFNPFISADGTRIGYHKCRGDSNGRKSNTLFLENVRSPVSNLSLFRIDGSFPSFSPAGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+A+V+FPGLYVI+RDGSN+++V+ GAAFSTAWDPVREGVVYTSAGPDFA +S+ VDIIS
Sbjct: 421 RIAFVNFPGLYVIHRDGSNQQKVYPGAAFSTAWDPVREGVVYTSAGPDFAPLSSQVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVDDDE N KKLT NG NNAFPSPSPDGKWIVFRSG++GYKNLYIMDAVDGE+K L  L
Sbjct: 481 VNVDDDESNIKKLTKNGENNAFPSPSPDGKWIVFRSGRSGYKNLYIMDAVDGENKALHRL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEGQWTDTMCSWSPDGDWIAF+SDR NPG+GSFDLFLIHPNGTGLRKLFQSGSAGRANHP
Sbjct: 541 TEGQWTDTMCSWSPDGDWIAFASDRHNPGAGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NWSPDGK LVFTTDNAGISAEPISNPHHYQPYGEI+ IK+DGS+LQRLTHNSYEDGTPTW
Sbjct: 601 NWSPDGKVLVFTTDNAGISAEPISNPHHYQPYGEIFTIKMDGSDLQRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYISP+NV+  YDVEPCGFEDCHWLNQK KA N+I+P LTGPRCS
Sbjct: 661 SPRYISPVNVQNSYDVEPCGFEDCHWLNQKNKAQNEIKPDLTGPRCS 702

BLAST of Lsi08G004570 vs. ExPASy TrEMBL
Match: A0A0A0KH20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G382360 PE=4 SV=1)

HSP 1 Score: 1258.0 bits (3254), Expect = 0.0e+00
Identity = 605/707 (85.57%), Postives = 639/707 (90.38%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MP LLL FLL FSSLL   SA V G     G+SIVFTTLGRS YAFDI+TLP D N  PS
Sbjct: 1   MPSLLLFFLLHFSSLLPAFSAKVSG-----GHSIVFTTLGRSFYAFDIYTLPADANKNPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
             DE  ITDGQ VNFNGYFPSS+SS SLISLLTNQS SF PD ELVYVTER+GIS IFYD
Sbjct: 61  LPDEILITDGQYVNFNGYFPSSTSSSSLISLLTNQSHSFTPDFELVYVTERNGISTIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           A+FGGIG+S RRRS+LEIPHRLQIPLLD+EQ+ E RVS KDRPSLSGDYL+YVSTHEDP 
Sbjct: 121 AVFGGIGVSARRRSELEIPHRLQIPLLDEEQKNEARVSFKDRPSLSGDYLVYVSTHEDPR 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSR+LKSG+TRRLTPYGIADFSPS+SPSGIWTAVASYGEKGWAG+VEELST
Sbjct: 181 ESRTSWAAVYSRNLKSGLTRRLTPYGIADFSPSVSPSGIWTAVASYGEKGWAGDVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           DLYIFLTRDGS RVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIY+AILPSHGEIS DSV
Sbjct: 241 DLYIFLTRDGSRRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYRAILPSHGEISLDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATS ANKNLIAVATRRPDSSFRHIELFN+VTGEFKELTK VSPNS
Sbjct: 301 IIERLTPPGLHVFTPATSSANKNLIAVATRRPDSSFRHIELFNIVTGEFKELTKTVSPNS 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPF+SADGT IGYHKCRGD N RKSN L FE VRSPVSNLSLFRIA SFPSFSP GD
Sbjct: 361 HHFNPFVSADGTRIGYHKCRGDGNRRKSNLLLFETVRSPVSNLSLFRIAVSFPSFSPAGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+AY +FPGLYVI RDGSN+REVFSGAAFSTAWDPVR+GVVYTSAGPDFA VS+ VDIIS
Sbjct: 421 RIAYANFPGLYVIERDGSNQREVFSGAAFSTAWDPVRKGVVYTSAGPDFAPVSSQVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVD++E+N KKLTTNG NNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAV+GESKGLR L
Sbjct: 481 VNVDEEERNIKKLTTNGENNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVEGESKGLRRL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEGQWTDTMCSWSP+GDWIAFSSDR+NPG GSFDLFLIHPNGTGLRKLFQSG  GRANHP
Sbjct: 541 TEGQWTDTMCSWSPEGDWIAFSSDRENPGGGSFDLFLIHPNGTGLRKLFQSGLGGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NW PDGK LVFTTDNAGIS EP+SNPHHYQPYGEIY IKLDGS+LQRLTHNSYEDGTPTW
Sbjct: 601 NWRPDGKALVFTTDNAGISGEPVSNPHHYQPYGEIYTIKLDGSDLQRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYI+P+NVE LYDVEPCGFEDCHWLNQ  KA N + P+LTGPRCS
Sbjct: 661 SPRYINPVNVESLYDVEPCGFEDCHWLNQNNKAHNHVSPVLTGPRCS 702

BLAST of Lsi08G004570 vs. NCBI nr
Match: XP_038884611.1 (uncharacterized protein LOC120075362 [Benincasa hispida])

HSP 1 Score: 1313.5 bits (3398), Expect = 0.0e+00
Identity = 628/707 (88.83%), Postives = 655/707 (92.64%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MPLL LCFLLQFSSLL  SSAA  GGD  SG+SIVFTTLGRS Y FDIFTLP DGNSYPS
Sbjct: 1   MPLLFLCFLLQFSSLLPASSAAATGGDARSGDSIVFTTLGRSYYTFDIFTLPADGNSYPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
           T  E Q+TDGQSVNFNG+FPS+S SPSLISLLTNQSQ  RPDLE+VYVTER+GISKIFYD
Sbjct: 61  T--EIQVTDGQSVNFNGHFPSASPSPSLISLLTNQSQFLRPDLEVVYVTERNGISKIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
            IFGG GIS RRRS+LEIPHRLQI LLDDEQE EVRVS KDRPSLSGDYL+YVSTH+DPG
Sbjct: 121 VIFGGTGISARRRSELEIPHRLQISLLDDEQENEVRVSFKDRPSLSGDYLVYVSTHDDPG 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           + RTSWAAVYS++LKSGITRRLTPYG+ADFSPS+SPSGIWTAVASYGE GWAGEVEELST
Sbjct: 181 KSRTSWAAVYSKNLKSGITRRLTPYGLADFSPSVSPSGIWTAVASYGENGWAGEVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           DLYIFLTRDGS RVKVVEHGGWPCW DDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV
Sbjct: 241 DLYIFLTRDGSRRVKVVEHGGWPCWGDDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
           KIERLTPPGLHVFTPATSP NKNLIAVATRRPDSSFRHIELFNLVTGEFKELTK VSPNS
Sbjct: 301 KIERLTPPGLHVFTPATSPTNKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKKVSPNS 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPFISADGT IGYHKCRG+ +GRKSNPLFFENVRSPVSNLSLFR  GSFPSFSP GD
Sbjct: 361 HHFNPFISADGTRIGYHKCRGEGDGRKSNPLFFENVRSPVSNLSLFRFIGSFPSFSPTGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+AY +FPGLYVIN DGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFA VS+ VDIIS
Sbjct: 421 RIAYANFPGLYVINPDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAPVSSEVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           +NVDDDE+N KKLTT G NNAFPSPSPDGKWIVFRSG+TGYKNLYIMDA+DGESKGL  L
Sbjct: 481 INVDDDERNIKKLTTKGENNAFPSPSPDGKWIVFRSGRTGYKNLYIMDAIDGESKGLHQL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEGQWTDTMCSWSPDGDWIAFSSDRDNPG GSFDLFLIHPNGTGLRKLFQSGSAGRANHP
Sbjct: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGGGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NWSP GK LVFTTDNAGISAEPISNPHHYQPYGEIY IK+DGS+L RLTHNSYEDGTPTW
Sbjct: 601 NWSPYGKVLVFTTDNAGISAEPISNPHHYQPYGEIYTIKMDGSDLHRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYISP+NVE LYD EPCGFEDCHWL QKKKA NDIRP+LTGPRCS
Sbjct: 661 SPRYISPVNVESLYDEEPCGFEDCHWLKQKKKAHNDIRPVLTGPRCS 705

BLAST of Lsi08G004570 vs. NCBI nr
Match: XP_023550097.1 (uncharacterized protein LOC111808393 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1280.4 bits (3312), Expect = 0.0e+00
Identity = 613/707 (86.70%), Postives = 650/707 (91.94%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MPL+ LCFLLQFSSLL PSSAA  G      NSIVFTTLGRS YAFD+FTLP D N  PS
Sbjct: 1   MPLVFLCFLLQFSSLLPPSSAAATG-----SNSIVFTTLGRSVYAFDVFTLPADENRSPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
             DET ITDG+SVNFNGYFPSSSSS S++SLLTNQSQS RPDLELVYVTER+GIS+IFYD
Sbjct: 61  AADETLITDGESVNFNGYFPSSSSSCSILSLLTNQSQSLRPDLELVYVTERNGISRIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           AI+GG G S RRRS LEIPHRLQIPLLDDEQ+ EVRVS KDRPSLSGDYL+YVSTHEDPG
Sbjct: 121 AIYGGTGFSARRRSALEIPHRLQIPLLDDEQKNEVRVSFKDRPSLSGDYLVYVSTHEDPG 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSRSL+SG TRRLTPYGIADFSPS+SPSG+WTAVASYGEKGWAGEVEELST
Sbjct: 181 ESRTSWAAVYSRSLRSGTTRRLTPYGIADFSPSVSPSGVWTAVASYGEKGWAGEVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           D+YIFLTRDG+ RVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIY+AILPS GEIS DSV
Sbjct: 241 DIYIFLTRDGTRRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYRAILPSDGEISTDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS
Sbjct: 301 IIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPFISADGT IGYHKCRGD NGRKSN LF ENVRSPVSNLSLFRI GSFPSFSP GD
Sbjct: 361 HHFNPFISADGTRIGYHKCRGDSNGRKSNTLFLENVRSPVSNLSLFRIDGSFPSFSPAGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+A+V+FPGLYVINRDGSN+++V+ GAAFSTAWDPVREGVVYTSAGPDFA +S+ VDIIS
Sbjct: 421 RIAFVNFPGLYVINRDGSNQQKVYPGAAFSTAWDPVREGVVYTSAGPDFAPLSSQVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVDDDE + KKLT NG NNAFPSPSPDGKWIVFRSG++GYKNLYIMDAV+GESK L  L
Sbjct: 481 VNVDDDESSIKKLTKNGENNAFPSPSPDGKWIVFRSGRSGYKNLYIMDAVEGESKELHRL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEGQWTDTMCSWSPDGDWIAF+SDR NPG+GSFDLFLIHPNGTGLRKLFQSGSAGRANHP
Sbjct: 541 TEGQWTDTMCSWSPDGDWIAFASDRHNPGAGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NWSPDGK LVFTTDNAGISAEPISNPHHYQPYGEIY IK+DGS+LQRLTHNSYEDGTPTW
Sbjct: 601 NWSPDGKVLVFTTDNAGISAEPISNPHHYQPYGEIYTIKMDGSDLQRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYISP+NV+  YDVEPCGFEDCHWLNQK KA N+I+P LTGPRCS
Sbjct: 661 SPRYISPVNVQNSYDVEPCGFEDCHWLNQKNKAQNEIKPDLTGPRCS 702

BLAST of Lsi08G004570 vs. NCBI nr
Match: XP_008466749.1 (PREDICTED: uncharacterized protein LOC103504088 [Cucumis melo])

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 613/707 (86.70%), Postives = 651/707 (92.08%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MP LLL FLL FSSLL PS AA  G     G+SIVFTTLGRS YAFDI+TLP D N+ PS
Sbjct: 1   MPSLLLLFLLHFSSLLPPSYAAASG-----GHSIVFTTLGRSFYAFDIYTLPADANNNPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
           + DE  ITDGQSVNFNGYFPSSSS  SLISLLTNQS SF PDLELVYVTER+GIS+IFYD
Sbjct: 61  SADEILITDGQSVNFNGYFPSSSSFSSLISLLTNQSHSFTPDLELVYVTERNGISRIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           A+FGG G+S RRRS+LEIPHRLQIPLLD+EQ+ EVRVS KDRPSLSGDYL+YVSTHEDP 
Sbjct: 121 AVFGGTGVSARRRSELEIPHRLQIPLLDEEQKNEVRVSFKDRPSLSGDYLVYVSTHEDPR 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSR+LKSG+TRRLTPYGIADFSPS+SPSGIWTAVASYGEKGWAGEVEELST
Sbjct: 181 EFRTSWAAVYSRNLKSGVTRRLTPYGIADFSPSVSPSGIWTAVASYGEKGWAGEVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           DLYIFLTRDGS RVKVVEHGGWPCWAD+STLYFHRRGDDQWLSIY+AILPSHGEI  DSV
Sbjct: 241 DLYIFLTRDGSRRVKVVEHGGWPCWADESTLYFHRRGDDQWLSIYRAILPSHGEIFLDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFN+VTGEFK+LT+ VSPN+
Sbjct: 301 IIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNIVTGEFKKLTETVSPNT 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPF+SADGT IGYHKCRGD NGRKSN LFFENVRSPVSNLSLFRIA SFPSFSP GD
Sbjct: 361 HHFNPFVSADGTRIGYHKCRGDGNGRKSNLLFFENVRSPVSNLSLFRIAVSFPSFSPAGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+AY +FPGLYVINRDGSN+REVFSGAAFSTAWDPVR+GVVYTSAGPDFA VS+ VDIIS
Sbjct: 421 RIAYANFPGLYVINRDGSNQREVFSGAAFSTAWDPVRKGVVYTSAGPDFAPVSSQVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVD++E+N KKLTTNG NNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLR L
Sbjct: 481 VNVDEEERNIKKLTTNGENNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRRL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEG WTDTMCSWSPDGDWIAFSSDR+NPG+GSFDLFLIHPNGTGLRKLFQSG  GRANHP
Sbjct: 541 TEGLWTDTMCSWSPDGDWIAFSSDRENPGAGSFDLFLIHPNGTGLRKLFQSGLGGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NW PDGK LVFTTDNAGISAEP+SNPHHYQPYGEIY IKLDGS+LQRLTHNSYEDGTPTW
Sbjct: 601 NWRPDGKALVFTTDNAGISAEPVSNPHHYQPYGEIYTIKLDGSDLQRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYI+P+NVE LYDVEPCGFEDCHWLNQ  +A NDIRP+LTGPRCS
Sbjct: 661 SPRYINPVNVENLYDVEPCGFEDCHWLNQNNRAHNDIRPVLTGPRCS 702

BLAST of Lsi08G004570 vs. NCBI nr
Match: KAA0055490.1 (DPP6 N-terminal domain-like protein [Cucumis melo var. makuwa] >TYK08970.1 DPP6 N-terminal domain-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1278.5 bits (3307), Expect = 0.0e+00
Identity = 613/707 (86.70%), Postives = 651/707 (92.08%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MP LLL FLL FSSLL PS AA  G     G+SIVFTTLGRS YAFDI+TLP D N+ PS
Sbjct: 50  MPSLLLLFLLHFSSLLPPSYAAASG-----GHSIVFTTLGRSFYAFDIYTLPADANNNPS 109

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
           + DE  ITDGQSVNFNGYFPSSSS  SLISLLTNQS SF PDLELVYVTER+GIS+IFYD
Sbjct: 110 SADEILITDGQSVNFNGYFPSSSSFSSLISLLTNQSHSFTPDLELVYVTERNGISRIFYD 169

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           A+FGG G+S RRRS+LEIPHRLQIPLLD+EQ+ EVRVS KDRPSLSGDYL+YVSTHEDP 
Sbjct: 170 AVFGGTGVSARRRSELEIPHRLQIPLLDEEQKNEVRVSFKDRPSLSGDYLVYVSTHEDPR 229

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSR+LKSG+TRRLTPYGIADFSPS+SPSGIWTAVASYGEKGWAGEVEELST
Sbjct: 230 EFRTSWAAVYSRNLKSGVTRRLTPYGIADFSPSVSPSGIWTAVASYGEKGWAGEVEELST 289

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           DLYIFLTRDGS RVKVVEHGGWPCWAD+STLYFHRRGDDQWLSIY+AILPSHGEI  DSV
Sbjct: 290 DLYIFLTRDGSRRVKVVEHGGWPCWADESTLYFHRRGDDQWLSIYRAILPSHGEIFLDSV 349

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFN+VTGEFK+LT+ VSPN+
Sbjct: 350 IIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNIVTGEFKKLTETVSPNT 409

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPF+SADGT IGYHKCRGD NGRKSN LFFENVRSPVSNLSLFRIA SFPSFSP GD
Sbjct: 410 HHFNPFVSADGTRIGYHKCRGDGNGRKSNLLFFENVRSPVSNLSLFRIAVSFPSFSPAGD 469

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+AY +FPGLYVINRDGSN+REVFSGAAFSTAWDPVR+GVVYTSAGPDFA VS+ VDIIS
Sbjct: 470 RIAYANFPGLYVINRDGSNQREVFSGAAFSTAWDPVRKGVVYTSAGPDFAPVSSQVDIIS 529

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVD++E+N KKLTTNG NNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLR L
Sbjct: 530 VNVDEEERNIKKLTTNGENNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRRL 589

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEG WTDTMCSWSPDGDWIAFSSDR+NPG+GSFDLFLIHPNGTGLRKLFQSG  GRANHP
Sbjct: 590 TEGLWTDTMCSWSPDGDWIAFSSDRENPGAGSFDLFLIHPNGTGLRKLFQSGLGGRANHP 649

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NW PDGK LVFTTDNAGISAEP+SNPHHYQPYGEIY IKLDGS+LQRLTHNSYEDGTPTW
Sbjct: 650 NWRPDGKALVFTTDNAGISAEPVSNPHHYQPYGEIYTIKLDGSDLQRLTHNSYEDGTPTW 709

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYI+P+NVE LYDVEPCGFEDCHWLNQ  +A NDIRP+LTGPRCS
Sbjct: 710 SPRYINPVNVENLYDVEPCGFEDCHWLNQNNRAHNDIRPVLTGPRCS 751

BLAST of Lsi08G004570 vs. NCBI nr
Match: XP_022938812.1 (uncharacterized protein LOC111444913 [Cucurbita moschata] >KAG6578947.1 hypothetical protein SDJN03_23395, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016471.1 tolB, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1277.3 bits (3304), Expect = 0.0e+00
Identity = 610/707 (86.28%), Postives = 649/707 (91.80%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           MPL+ LCFLLQFSSLL PSSAA  G       SIVFTTLGRS YAFD+FTLP D N  PS
Sbjct: 1   MPLVFLCFLLQFSSLLPPSSAAASG-----SGSIVFTTLGRSVYAFDVFTLPADENRAPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
             DET ITDG+SVNFNGYFPSSSSS S++SLLTNQSQS RPDLELVYVTER+GIS+IFYD
Sbjct: 61  AADETLITDGESVNFNGYFPSSSSSSSILSLLTNQSQSLRPDLELVYVTERNGISRIFYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLDDEQEKEVRVSLKDRPSLSGDYLLYVSTHEDPG 180
           AIFGG G S RRRS LEIPHRLQIPL+DDEQ+ EVRVS KDRP+LSGDYL+YVSTHEDPG
Sbjct: 121 AIFGGTGFSARRRSALEIPHRLQIPLVDDEQKNEVRVSFKDRPNLSGDYLVYVSTHEDPG 180

Query: 181 EPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELST 240
           E RTSWAAVYSRSL+SG TRRLTPYGIADFSPS+SPSG+WTAVASYGEKGWAGEVEELST
Sbjct: 181 ESRTSWAAVYSRSLRSGTTRRLTPYGIADFSPSVSPSGVWTAVASYGEKGWAGEVEELST 240

Query: 241 DLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEISPDSV 300
           D+YIFLTRDG+ RVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIY+AILPS GEIS DSV
Sbjct: 241 DIYIFLTRDGTRRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYRAILPSDGEISTDSV 300

Query: 301 KIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360
            IERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS
Sbjct: 301 IIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSPNS 360

Query: 361 HHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPVGD 420
           HH NPFISADGT IGYHKCRGD NGRKSN LF ENVRSPVSNLSLFRI GSFPSFSP GD
Sbjct: 361 HHFNPFISADGTRIGYHKCRGDGNGRKSNTLFLENVRSPVSNLSLFRIDGSFPSFSPAGD 420

Query: 421 RVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIIS 480
           R+A+V+FPGLYVINRDGSN+++V+ GAAFSTAWDPVREGVVYTSAGPDFA +S+ VDIIS
Sbjct: 421 RIAFVNFPGLYVINRDGSNQQKVYPGAAFSTAWDPVREGVVYTSAGPDFAPLSSQVDIIS 480

Query: 481 VNVDDDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKGLRPL 540
           VNVDDDE+  K+LT NG NNAFPSPSPDGKWIVFRSG++GYKNLYIMDAVDGE+K L  L
Sbjct: 481 VNVDDDERTIKRLTKNGENNAFPSPSPDGKWIVFRSGRSGYKNLYIMDAVDGENKALHRL 540

Query: 541 TEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600
           TEGQWTDTMCSWSPDGDWIAF+SDR NPG+GSFDLFLIHPNGTGLRKLFQSGSAGRANHP
Sbjct: 541 TEGQWTDTMCSWSPDGDWIAFASDRHNPGAGSFDLFLIHPNGTGLRKLFQSGSAGRANHP 600

Query: 601 NWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 660
           NWSPDGK LVFTTDNAGISAEPISNPHHYQPYGEIY IK+DGS+LQRLTHNSYEDGTPTW
Sbjct: 601 NWSPDGKVLVFTTDNAGISAEPISNPHHYQPYGEIYTIKMDGSDLQRLTHNSYEDGTPTW 660

Query: 661 SPRYISPINVEGLYDVEPCGFEDCHWLNQKKKADNDIRPILTGPRCS 708
           SPRYISP+NV+  YDVEPCGFEDCHWLNQK KA N+I+P LTGPRCS
Sbjct: 661 SPRYISPVNVQNSYDVEPCGFEDCHWLNQKNKAQNEIKPDLTGPRCS 702

BLAST of Lsi08G004570 vs. TAIR 10
Match: AT1G21680.1 (DPP6 N-terminal domain-like protein )

HSP 1 Score: 909.4 bits (2349), Expect = 1.8e-264
Identity = 440/694 (63.40%), Postives = 538/694 (77.52%), Query Frame = 0

Query: 9   LLQFSSLLLP---SSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPSTEDET 68
           ++ F+SLLL    S+A     +   G++I+FTTLGRS Y FDIF L       PS   E 
Sbjct: 6   IIFFASLLLRLHLSTAEHQNSNAGDGDTIIFTTLGRSHYEFDIFALST--TQPPSVSGEL 65

Query: 69  QITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPD---LELVYVTERDGISKIFYDAI 128
           +ITDG+SVNFNGYFP  S SP+L+SLL +++     D   L L+YVTER+G S ++YD +
Sbjct: 66  RITDGESVNFNGYFP--SPSPALLSLLPDETLIQMEDSSPLHLIYVTERNGTSSLYYDLV 125

Query: 129 FGG-IGISVRRRSKLEIPHRLQIPLLD--DEQEKEVRVSLKDRPSLSGDYLLYVSTHEDP 188
           +GG      +RRS LE P R+Q+PLL   D        S KD+PSLSG++++YVSTHE  
Sbjct: 126 YGGNSDFKTKRRSVLEAPSRVQVPLLSRFDHLSGMTVNSFKDKPSLSGEFIVYVSTHESS 185

Query: 189 GEPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEVEELS 248
           GEPR SW AVYS  LK+G+TRRLTP G+ADFSP++SPSG  TAVASYGE+GW GEVEEL 
Sbjct: 186 GEPRASWTAVYSTELKTGLTRRLTPSGVADFSPAVSPSGNLTAVASYGERGWTGEVEELR 245

Query: 249 TDLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRG-DDQWLSIYKAILPSHGEISPD 308
           TD+Y+FLTRDGSHRVKVVEHGGWPCW D+STLYFHRR  +D W+S+Y+AILP +G ++ +
Sbjct: 246 TDIYVFLTRDGSHRVKVVEHGGWPCWVDESTLYFHRRSEEDGWISVYRAILPENGPLTTE 305

Query: 309 SVKIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAVSP 368
           SV I+R+TPPG+H FTPATSP N   +AVATRRP S +RH+ELF+L   EF ELT+ V+P
Sbjct: 306 SVTIQRVTPPGVHAFTPATSPNNHEFVAVATRRPGSDYRHVELFDLKRNEFIELTRLVAP 365

Query: 369 NSHHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFSPV 428
            SHHLNPF+S D + +GYH CRGD NGR+S  LF EN+++   +LSLFRI GSFPSFSP 
Sbjct: 366 KSHHLNPFLSPDSSRVGYHSCRGDANGRRSPLLFLENIQTTTRDLSLFRIDGSFPSFSPG 425

Query: 429 GDRVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIVDI 488
           GDR+AYV  PG++V+  DGS +REV+ G AFSTAWDPVR G+VY+S+GP FA   T VD+
Sbjct: 426 GDRIAYVKMPGVFVVKPDGSGQREVYKGMAFSTAWDPVRPGIVYSSSGPTFATERTEVDV 485

Query: 489 ISVNVD--DDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESKG 548
           IS++VD  D   + ++LTTNG NNAFP PSPDGK IVFRSG+TG+KNLYIMDA  GES G
Sbjct: 486 ISIDVDAADKSSSVRRLTTNGKNNAFPWPSPDGKRIVFRSGRTGHKNLYIMDAEKGESGG 545

Query: 549 LRPLTEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSAGR 608
           L  LTEG WTDTMC+WSPDG+WIAF+SDR++PGSGSF+LFLIHPNGTGLRKL QSG+ GR
Sbjct: 546 LWRLTEGAWTDTMCNWSPDGEWIAFASDRESPGSGSFELFLIHPNGTGLRKLIQSGTGGR 605

Query: 609 ANHPNWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDG 668
            NHP +SPD K LVFT+D AGISAEPISNPHHYQPYG+I+ +KLDGSN++RLTHNSYEDG
Sbjct: 606 TNHPIFSPDSKSLVFTSDYAGISAEPISNPHHYQPYGDIFTVKLDGSNVRRLTHNSYEDG 665

Query: 669 TPTWSPRYISPINVE-GLYDVEPCGFEDCHWLNQ 690
           TP W+PR+I P NVE    +   C FEDCHWLN+
Sbjct: 666 TPAWAPRFIHPNNVELQRRNDSRCSFEDCHWLNK 695

BLAST of Lsi08G004570 vs. TAIR 10
Match: AT1G21670.1 (LOCATED IN: cell wall, plant-type cell wall; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: WD40-like Beta Propeller (InterPro:IPR011659), Six-bladed beta-propeller, TolB-like (InterPro:IPR011042); BEST Arabidopsis thaliana protein match is: DPP6 N-terminal domain-like protein (TAIR:AT1G21680.1); Has 8461 Blast hits to 5060 proteins in 1257 species: Archae - 79; Bacteria - 5567; Metazoa - 37; Fungi - 70; Plants - 117; Viruses - 0; Other Eukaryotes - 2591 (source: NCBI BLink). )

HSP 1 Score: 714.1 bits (1842), Expect = 1.1e-205
Identity = 358/669 (53.51%), Postives = 465/669 (69.51%), Query Frame = 0

Query: 1   MPLLLLCFLLQFSSLLLPSSAAVIGGDIISGNSIVFTTLGRSSYAFDIFTLPVDGNSYPS 60
           M L    F      LL  SSA+     + +G++I+FTT+GR ++ FDIFTLP   +  PS
Sbjct: 1   MKLTNALFFFFLICLLSLSSASSNKPQLSNGSTILFTTIGRPTFEFDIFTLPT-SHRPPS 60

Query: 61  TEDETQITDGQSVNFNGYFPSSSSSPSLISLLTNQSQSFRPDLELVYVTERDGISKIFYD 120
             DE ++TDG+S+NFNGYF  +S S +LISLL  ++Q    D+ L+YVTER G   + YD
Sbjct: 61  PADEHRLTDGKSINFNGYF--ASPSTALISLLPKRTQIQPQDVHLIYVTERAGTPSLNYD 120

Query: 121 AIFGGIGISVRRRSKLEIPHRLQIPLLD-DEQEKEVRV-SLKDRPSLSGDYLLYVSTHED 180
            +               +  R+Q+PL   +EQ+  + V S+KD P L+  YL++VSTHE+
Sbjct: 121 VVHSD-----------NVGSRIQVPLFSGEEQQSGMNVNSMKDTPVLTNGYLVHVSTHEN 180

Query: 181 PGEPRTSWAAVYSRSLKSGITRRLTPYGIADFSPSISPSGIWTAVASYGEKGWAGEV--E 240
           PG+P  SWAAVYS  L++  TRRLTP GIADFSP++SPSG WTAVAS+GEKGW   +  +
Sbjct: 181 PGKPMASWAAVYSTELRTKSTRRLTPLGIADFSPAVSPSGKWTAVASFGEKGWTWSMVEK 240

Query: 241 ELSTDLYIFLTRDGSHRVKVVEHGGWPCWADDSTLYFHRRGDDQWLSIYKAILPSHGEIS 300
           E+S+D+Y+FLT+DG+ RVKVVE GGWP W DDSTLYFHR+ DD W+S+Y+AILP  G ++
Sbjct: 241 EISSDVYVFLTQDGTQRVKVVEQGGWPRWVDDSTLYFHRKSDDGWISVYRAILPKTGPVT 300

Query: 301 PDSVKIERLTPPGLHVFTPATSPANKNLIAVATRRPDSSFRHIELFNLVTGEFKELTKAV 360
             SV I+R+TPPGLH FTPATSP N N IAVATRRP S  RH+ELF+L   EF ELT+ V
Sbjct: 301 TKSVTIQRVTPPGLHAFTPATSPNNNNFIAVATRRPGSEIRHVELFDLKKNEFVELTRLV 360

Query: 361 SPNSHHLNPFISADGTHIGYHKCRGDDNGRKSNPLFFENVRSPVSNLSLFRIAGSFPSFS 420
           SP SHH NPF+S D + +GYH CRGD  GRK+     +++++  ++LSLFR  G+FPS S
Sbjct: 361 SPKSHHFNPFLSPDSSRVGYHSCRGDATGRKTPRNLLQSLKTTSNDLSLFRFDGAFPSIS 420

Query: 421 PVGDRVAYVDFPGLYVINRDGSNKREVFSGAAFSTAWDPVREGVVYTSAGPDFAAVSTIV 480
           P GDR A+V F G++V+N DGS  R++     F T WDP+R G+VYTS+GP  A   + +
Sbjct: 421 PEGDRFAFVSFTGVFVVNPDGSGLRQLLPQMGFGTVWDPIRHGIVYTSSGPALAPGKSQI 480

Query: 481 DIISVNVD--DDEKNFKKLTTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGES 540
           DI+++NVD        KKLTT G NNAFP PSPDGK IVFRS ++G KNLYIMDA  GES
Sbjct: 481 DILAINVDAPSPATAVKKLTTTGENNAFPWPSPDGKRIVFRSARSGTKNLYIMDAEKGES 540

Query: 541 KGLRPLTEGQWTDTMCSWSPDGDWIAFSSDRDNPGSGSFDLFLIHPNGTGLRKLFQSGSA 600
            GL  LT G W DT+ +WSPDG+WI F+S+R+ PG+   +++++HP+GTGLRKL Q+ + 
Sbjct: 541 GGLFRLTNGNWNDTIATWSPDGNWIVFASNREFPGTLLMNIYVVHPDGTGLRKLAQNLTG 600

Query: 601 GRANHPNWSPDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYE 660
             + HP +SPD K +VFTT  AGISAE I NPH   P  EI+ + LDGS L RLTHNS E
Sbjct: 601 LVSMHPMFSPDSKRIVFTTIYAGISAEQIGNPHFNVPSSEIFTVNLDGSGLTRLTHNSVE 655

Query: 661 DGTPTWSPR 664
           DG P W P+
Sbjct: 661 DGPPMWFPK 655

BLAST of Lsi08G004570 vs. TAIR 10
Match: AT4G01870.1 (tolB protein-related )

HSP 1 Score: 469.9 bits (1208), Expect = 3.6e-132
Identity = 266/657 (40.49%), Postives = 379/657 (57.69%), Query Frame = 0

Query: 33  SIVFTTLGRSSYAFDIFTLPVDGNSYPSTEDETQITDGQSVNFNGYFPSSSSSPSLISLL 92
           +I+FTT+GR+ Y FD+F+L +      +T  E ++TDG SVNFN  F +  S        
Sbjct: 7   TIIFTTVGRTHYGFDVFSLNI------ATSVERRLTDGVSVNFNAQFVNDKSD------- 66

Query: 93  TNQSQSFRPDLELVYVTERDGISKIFYDAIFGGIGISVRRRSKLEIPHRLQIPLLDDEQE 152
                      ++V+V+ER+G ++I+            + RS +  P   QIP   +   
Sbjct: 67  -----------DVVFVSERNGSARIY------------KTRSGISKPE--QIPGAPESY- 126

Query: 153 KEVRVSLKDRPSLSGDYLLY-VSTHEDPGEPRTSWAAVYSRSLKSG--ITRRLTPYGIAD 212
                   DRP ++ +  LY +S HE P     +W+A+Y+  L S      R+TP   AD
Sbjct: 127 ------FHDRPIITQNNRLYFISAHEQPDRYFKNWSALYTVELNSAKREVTRVTPPDTAD 186

Query: 213 FSPSISPSGIWTAVASYGEKGWAGEVEELSTDLYIFLTRDGSHRVKVVEHGGWPCWADDS 272
           FSP++S SG + AVASYG + W GE  E++TD+ +F       RV + E GGWP W+ DS
Sbjct: 187 FSPAVSQSGDFLAVASYGTRSWGGEFHEINTDITVFKASKPETRVVICERGGWPTWSGDS 246

Query: 273 TLYFHRRGDDQWLSIYKAILPSH-GEISPDSVKIERLTPPGLHVFTPATSPANKNLIAVA 332
           T++FH + DD W SI++  +P +  E +   +   R+TP GLH FTPA     K  IA+A
Sbjct: 247 TVFFHHQADDGWWSIFRVDIPENFTEYTDFPITPIRVTPSGLHCFTPAAFRDGKR-IALA 306

Query: 333 TRRPDSSFRHIELFNLVTGEFKELTKAVSPNSHHLNPFISADGTHIGYHKCRGDDNGRKS 392
           TRR   + RHIE+++L    F+ +T++++P+ HH NPF+S D   +GYH+ RG+    +S
Sbjct: 307 TRRRGVNHRHIEIYDLENTTFQPVTESLNPSFHHYNPFVSPDSEFLGYHRFRGESTQGES 366

Query: 393 NPLFFENVRSPVSNLSLFRIAGSFPSFSPVGDRVAY-VDFP---GLYVINRDGSNKREVF 452
                E++ SP+  L L RI GSFPS SP GD +A   DF    G+ V   DGS +  + 
Sbjct: 367 IVPNIESIVSPIKTLRLLRINGSFPSSSPNGDLIALNSDFDINGGIKVSKSDGSKRWTLI 426

Query: 453 SG-AAFSTAWDPVREGVVYTSAGPDFAAVSTIVDIISVNVDDDEKNFKK----------L 512
               AF  +W P    V+YTS GP F+     V I  +  D  +    K           
Sbjct: 427 KDRTAFYNSWSPTERHVIYTSLGPIFSPARIAVQIARIKFDPSDLTADKEDLPCDVKILT 486

Query: 513 TTNGVNNAFPSPSPDGKWIVFRSGQTGYKNLYIMDAVDGESK--GLRPLTEGQWTDTMCS 572
             N  NNAFPS SPDGK IVFRSG++G+KNLYI+DAV+GES   G+R LT+G W DTM  
Sbjct: 487 LENTGNNAFPSCSPDGKSIVFRSGRSGHKNLYIVDAVNGESNGGGIRRLTDGPWIDTMPC 546

Query: 573 WSPDGDWIAFSSDRDNP-GSGSFDLFLIHPNGTGLRKLFQSGSAG-------RANHPNWS 632
           WSP GD I FSS+R NP  +  F  +++ P+GTGLR++  SG  G       R NH +++
Sbjct: 547 WSPKGDLIGFSSNRHNPENTAVFGAYVVRPDGTGLRRIQISGPEGSEEAARERVNHVSFN 606

Query: 633 PDGKFLVFTTDNAGISAEPISNPHHYQPYGEIYIIKLDGSNLQRLTHNSYEDGTPTW 661
            DG +LVF  + +G++AEP++ P+ +QPYG++Y++KLDG+ L+RLT N YEDGTPTW
Sbjct: 607 KDGDWLVFAANLSGVTAEPVTMPNQFQPYGDLYVVKLDGTGLRRLTWNGYEDGTPTW 617