Lsi08G004100 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G004100
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	flavonol synthase/flavanone 3-hydroxylase
Location	chr08: 11506602 .. 11508137 (-)
RNA-Seq Expression	Lsi08G004100
Synteny	Lsi08G004100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGGTAATAAATAGTATTATAGCGGATAGGAGGAGGCTACGCGCCTTTAATTCTGTTCTGAAAAGACGCATCTAACTCGAGAAGTTTGTTGGAACCGTATCCATTGAAAGCAACCCTTCTAGCCTCTCTGTTTTTTGATGGCAGTTCCATCCATTTTAAACAAAACCCCATCTCTTCCTTCTCCCTCACCAAATTCATAGCCAAATTTCAAAAATGGAGGTCGGGAGAGTCCAGGATATCGCTTCCTCGGTGCTTTTCTCCGACAATCTCCCCTCCGAGTTCGTTCGTCCGGAGACAGAGCAGCCCGGAATCACCACCGTCAAAGGCCAGGTCCTCGCCGTTCCTGTCATCGATTTCTCTAACCCAGATGAGGACTCTGTTCTCCGAGCCATCGACGACGCCTCTCGCAATTGGGGCATGTTTCAAATCATTAACCACGAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTCGGGAAGGAATTCTTCGAGCTCCCACCCGAGGAGAAAGAGGTTTACGCCAAACCCCCTGGTTCGAAATCTGTCGAAGGCTATGGAACTAAGCTCCAAAAAGAAATCGATGGAAAGAAGGGGTGGGTCGACCATTTGTTCCACATAGTTTGGCCTTCTTCTGCCATTAACTACAATTTCTGGCCCCAAAATCCCCCTTCTTACAGGTACAAATTCCAAATTGTTGAAGGATCGTACATCTTGATTTGTTAGTTACTCTGTTTTTGGGTTTGTCTGATCTGATGCAGGGCAGCGAATGAGGAGTACAGAAAGCACTTACATGGAGTTGTGGAGAAGCTATTCAGAAGCCTCTCGCTAGGGCTGGGGCTTGAAGGGCACGAACTAAAAGAGGGAGCCGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTACCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCGGCCATTACAATTCTTGTGCCGAATGAAGTTCAAGGCCTTCAGGCGTTTAGAGAGGGGCATTGGTACGACGTCAATTACATCCCTAATGCCCTCGTTATCCACATCGGGGATCAAATGGAGGTAAATCGTTTCTTCAACTTCAGAGATTTTTTTTTTTTTAATTTTCTGTTGTGGAAAATGGCTGAATTAGAAGTGTGTTGTGTTGTGAGGGCAGATTCTGAGTAATGGAAAGTATAAGAGTGTATTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCCCCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGCCAAAATATAAGACGAAGAAATACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCCCAGTGACTCAAAACACCGCCGGGCCTGAGATTCTGCAGCATCTTACTTGGTCGTCGTCTGTTTTGTCTTCTCCGGCCGCCGCGTTCTGCTTACTGCTTATAATGTCTCAAATTAATGCAACAAGGGTTGGAGGGTAACGTTTTTTT

mRNA sequence

CGGGTAATAAATAGTATTATAGCGGATAGGAGGAGGCTACGCGCCTTTAATTCTGTTCTGAAAAGACGCATCTAACTCGAGAAGTTTGTTGGAACCGTATCCATTGAAAGCAACCCTTCTAGCCTCTCTGTTTTTTGATGGCAGTTCCATCCATTTTAAACAAAACCCCATCTCTTCCTTCTCCCTCACCAAATTCATAGCCAAATTTCAAAAATGGAGGTCGGGAGAGTCCAGGATATCGCTTCCTCGGTGCTTTTCTCCGACAATCTCCCCTCCGAGTTCGTTCGTCCGGAGACAGAGCAGCCCGGAATCACCACCGTCAAAGGCCAGGTCCTCGCCGTTCCTGTCATCGATTTCTCTAACCCAGATGAGGACTCTGTTCTCCGAGCCATCGACGACGCCTCTCGCAATTGGGGCATGTTTCAAATCATTAACCACGAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTCGGGAAGGAATTCTTCGAGCTCCCACCCGAGGAGAAAGAGGTTTACGCCAAACCCCCTGGTTCGAAATCTGTCGAAGGCTATGGAACTAAGCTCCAAAAAGAAATCGATGGAAAGAAGGGGTGGGTCGACCATTTGTTCCACATAGTTTGGCCTTCTTCTGCCATTAACTACAATTTCTGGCCCCAAAATCCCCCTTCTTACAGGGCAGCGAATGAGGAGTACAGAAAGCACTTACATGGAGTTGTGGAGAAGCTATTCAGAAGCCTCTCGCTAGGGCTGGGGCTTGAAGGGCACGAACTAAAAGAGGGAGCCGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTACCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCGGCCATTACAATTCTTGTGCCGAATGAAGTTCAAGGCCTTCAGGCGTTTAGAGAGGGGCATTGGTACGACGTCAATTACATCCCTAATGCCCTCGTTATCCACATCGGGGATCAAATGGAGATTCTGAGTAATGGAAAGTATAAGAGTGTATTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCCCCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGCCAAAATATAAGACGAAGAAATACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCCCAGTGACTCAAAACACCGCCGGGCCTGAGATTCTGCAGCATCTTACTTGGTCGTCGTCTGTTTTGTCTTCTCCGGCCGCCGCGTTCTGCTTACTGCTTATAATGTCTCAAATTAATGCAACAAGGGTTGGAGGGTAACGTTTTTTT

Coding sequence (CDS)

ATGGAGGTCGGGAGAGTCCAGGATATCGCTTCCTCGGTGCTTTTCTCCGACAATCTCCCCTCCGAGTTCGTTCGTCCGGAGACAGAGCAGCCCGGAATCACCACCGTCAAAGGCCAGGTCCTCGCCGTTCCTGTCATCGATTTCTCTAACCCAGATGAGGACTCTGTTCTCCGAGCCATCGACGACGCCTCTCGCAATTGGGGCATGTTTCAAATCATTAACCACGAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTCGGGAAGGAATTCTTCGAGCTCCCACCCGAGGAGAAAGAGGTTTACGCCAAACCCCCTGGTTCGAAATCTGTCGAAGGCTATGGAACTAAGCTCCAAAAAGAAATCGATGGAAAGAAGGGGTGGGTCGACCATTTGTTCCACATAGTTTGGCCTTCTTCTGCCATTAACTACAATTTCTGGCCCCAAAATCCCCCTTCTTACAGGGCAGCGAATGAGGAGTACAGAAAGCACTTACATGGAGTTGTGGAGAAGCTATTCAGAAGCCTCTCGCTAGGGCTGGGGCTTGAAGGGCACGAACTAAAAGAGGGAGCCGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTACCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCGGCCATTACAATTCTTGTGCCGAATGAAGTTCAAGGCCTTCAGGCGTTTAGAGAGGGGCATTGGTACGACGTCAATTACATCCCTAATGCCCTCGTTATCCACATCGGGGATCAAATGGAGATTCTGAGTAATGGAAAGTATAAGAGTGTATTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTTTTGGAGCCCCCGCCGGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGCCAAAATATAAGACGAAGAAATACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCCCAGTGA

Protein sequence

MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQKEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ

Homology

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BLAST of Lsi08G004100 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 7.3e-149
Identity = 242/336 (72.02%), Postives = 283/336 (84.23%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQ IAS     D +PSE++R E EQP  TT+ G VL VPVID  +PDE+ +++ I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFE-LPPEEKEVYAKPPGSKSVEGYGTKL 120
            DAS+ WG+FQ+INH IP+EAI  LQ VGKEFFE +P EEKE+ AK PGS  +EGYGT L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 121 QKEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSL 180
           QKE++GKKGWVDHLFH +WP SA+NY +WP+NPPSYR ANEEY K +  VV+++F+SLSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 181 GLGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQ 240
           GLGLEGHE+ E AGGDE+VYLLKINYYPPCPRPDLALGV AHTDMS ITILVPNEVQGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 241 AFREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPE 300
            F++GHWYDV YIPNAL++HIGDQ+EILSNGKYKSV+HRTTVNK+KTR+SWPVFLEPP E
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 301 FEVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
            EVGP PKL+ E NPPK+KTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

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BLAST of Lsi08G004100 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.0e-146
Identity = 238/335 (71.04%), Postives = 282/335 (84.18%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQ+IAS     D +P+E++R E EQP I+TV G VL VPVID S+ DE  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
            +AS+ WG+FQ++NH IPNE I  LQ VGK FFELP EEKE+ AKP GS+S+EGYGT+LQ
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGKKGWVDHLFH +WP SAINY FWP+NPP+YR ANEEY K L  VV+ LF+ LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           L LE +  K+GAGGD+LVYL+KINYYPPCPRPDLALGV AHTDMSAIT+LVPNEV GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           +++GHWYD  YIPNAL++HIGDQ+EI+SNGKYKSV+HRTTVNKEKTR+SWPVFLEPPP+ 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           EVGP PKLV+EENP K+KTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

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BLAST of Lsi08G004100 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 6.4e-145
Identity = 238/321 (74.14%), Postives = 272/321 (84.74%), Query Frame = 0


Query: 19  LPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIINHEIP 78
           +P+EF+R E EQPGITTV G+VL VP+IDFS+PDE+ ++  I +AS NWGM+QI+NH+IP
Sbjct: 17  IPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHDIP 76

Query: 79  NEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQKEID----GKKGWVDHLF 138
           +E I+ LQAVGKEFFELP EEKE YAKPP S S+EGYGTKL KEI      KKGWVD+LF
Sbjct: 77  SEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDNLF 136

Query: 139 HIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKEGAGG 198
           + +WP S +NY FWP+NPPSYR ANEEY KHLH VVEKLFR LSLGLGLEG ELK+ AGG
Sbjct: 137 NKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAAGG 196

Query: 199 DELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFREGHWYDVNYIPN 258
           D L YLLKINYYPPCPRPDLALGV AHTDMS +TILVPN+VQGLQA ++G WYDV YIPN
Sbjct: 197 DNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYIPN 256

Query: 259 ALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENP 318
           ALVIHIGDQMEI+SNGKY SV HRTTVNK+KTRISWPVFLEPP +  VGPHP+LV+  N 
Sbjct: 257 ALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAVNQ 316

Query: 319 PKYKTKKYSEYAYCKLNKIPQ 336
           PKYKTKKY +Y YCK+NK+PQ
Sbjct: 317 PKYKTKKYGDYVYCKINKLPQ 337

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BLAST of Lsi08G004100 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 8.3e-145
Identity = 236/337 (70.03%), Postives = 280/337 (83.09%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDF--SNPDEDSVLR 60
           MEV RVQ I+      D +PSE++R E+EQP +TT++G VL VPVID   SN  E++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  AIDDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTK 120
            I +ASR WG+FQ++NH IP++AI  LQ VGKEFFELP +EKEV AKP G + VEGYGTK
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 121 LQKEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLS 180
           LQKE+ GKKGWVDHLFHI+WP SA+NYNFWP NPP YR ANEEY   L GVV+KLF +LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 181 LGLGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGL 240
           LG+GLE HELK+ +GGD+L+Y+LKINYYPPCPRPDLALGV AHTDMSAITILVPNEVQGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 241 QAFREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPP 300
           Q  ++ HWYDV YIPNAL+IHIGDQ+EI+SNGKYKSV+HRTTVNK+KTR+SWPVFLEPPP
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 301 EFEVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           E   GP  KL+ +ENP K+KTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

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BLAST of Lsi08G004100 vs. ExPASy Swiss-Prot
Match: Q41452 (Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 2.1e-140
Identity = 233/338 (68.93%), Postives = 279/338 (82.54%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSN--PDEDSVLR 60
           MEV RVQ I+S     D +PSE++R E EQP  TT++G VL VPVID SN   DE+ +++
Sbjct: 15  MEVARVQAISSITKCMDTIPSEYIRSENEQPAATTLQGVVLEVPVIDISNVDDDEEKLVK 74

Query: 61  AIDDASRNWGMFQIINHEIPNEAITHLQAVGKEFF-ELPPEEKEVYAKPPGSKSVEGYGT 120
            I +AS+ WG+FQ+INH IP+E I +LQ VGKEFF E+P EEKE+ AK PG++S+EGYGT
Sbjct: 75  EIVEASKEWGIFQVINHGIPDEVIENLQKVGKEFFEEVPQEEKELIAKKPGAQSLEGYGT 134

Query: 121 KLQKEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSL 180
            LQKEI+GKKGWVDHLFH +WP SAINY +WP+NPPSYR ANEEY K L  V + +FRSL
Sbjct: 135 SLQKEIEGKKGWVDHLFHKIWPPSAINYRYWPKNPPSYREANEEYAKWLRKVADGIFRSL 194

Query: 181 SLGLGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQG 240
           SLGLGLEGHE+ E AG +++VY+LKINYYPPCPRPDLALGV AHTDMS IT+LVPNEV  
Sbjct: 195 SLGLGLEGHEMMEAAGSEDIVYMLKINYYPPCPRPDLALGVVAHTDMSYITLLVPNEV-- 254

Query: 241 LQAFREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPP 300
            Q F++GHWYDVNYIPNA+++HIGDQ+EILSNGKYKSV+HRTTVNK KTR+SWPVFLEP 
Sbjct: 255 -QVFKDGHWYDVNYIPNAIIVHIGDQVEILSNGKYKSVYHRTTVNKYKTRMSWPVFLEPS 314

Query: 301 PEFEVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
            E EVGP P L++E NPPK+KTKKY +Y YCKLNK+PQ
Sbjct: 315 SEHEVGPIPNLINEANPPKFKTKKYKDYVYCKLNKLPQ 349

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BLAST of Lsi08G004100 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.0e-185
Identity = 307/335 (91.64%), Postives = 323/335 (96.42%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           M+VGRVQ++ASS+LFS  LPSEFVRPETEQPGITTVKGQVLAVPVIDF+NPD+DSVL AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
            DAS NWGMFQIINH+IPNEAITHLQAVGKEFFELPPEEKE+YAKPP SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHI+WP S+INYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWYDVNYIPNALVIHIGDQ+EILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENP KYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

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BLAST of Lsi08G004100 vs. ExPASy TrEMBL
Match: A0A1S3BCS7 (flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103488630 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.0e-185
Identity = 307/335 (91.64%), Postives = 323/335 (96.42%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           M+VGRVQ++ASS+LFS  LPSEFVRPETEQPGITTVKGQVLAVPVIDF+NPD+DSVL AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
            DAS NWGMFQIINH+IPNEAITHLQAVGKEFFELPPEEKE+YAKPP SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHI+WP S+INYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWYDVNYIPNALVIHIGDQ+EILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENP KYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

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BLAST of Lsi08G004100 vs. ExPASy TrEMBL
Match: A0A0A0K885 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040540 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.1e-183
Identity = 304/335 (90.75%), Postives = 321/335 (95.82%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQ++ASS+LFS  LPS+FVRP+TEQPGITTVKGQVLAVPVIDF+NPD+DSVL AI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
            DAS NWGMFQIINH+IPNEAITHLQAVGKEFF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHI+WP S+INYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWYDVNYIPNALVIHIGDQ+EILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVDE+ P +YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Lsi08G004100 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 6.8e-182
Identity = 299/335 (89.25%), Postives = 320/335 (95.52%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIASS LFS +LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
           DDASRNWGMFQIINH+IPNEAI HLQAVGK FFELPPEEKEVYAKPP SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DG+KGWVDHLFHI+WP SAIN+ FWPQNPPSYRAANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWY+VNYIPNALVIHIGDQ+EILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Lsi08G004100 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.7e-180
Identity = 296/335 (88.36%), Postives = 318/335 (94.93%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIASS LFS ++P+EFVRP+ EQPGITTVKGQ+LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
           DDASRNWGMFQIINH+IPNEAI HLQAVGK FFELPPEEKEVYAKPP SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGKKGWVDHLFHI+WP SAIN+ FWPQNPPSYR ANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELK+ AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWY+VNYIPNALVIHIGDQ+EILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Lsi08G004100 vs. NCBI nr
Match: XP_038885635.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 672.2 bits (1733), Expect = 2.4e-189
Identity = 318/334 (95.21%), Postives = 327/334 (97.90%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIASSVL+SD LP+EFVR ETEQPGITTVKGQVLAVPVIDF++PD+DSVLRAI
Sbjct: 1   MEVGRVQDIASSVLYSDTLPAEFVRSETEQPGITTVKGQVLAVPVIDFTDPDDDSVLRAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
           DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKKGWVDHLFHI+WP SAINYNFWPQNPPSYRAANEEY KHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKKGWVDHLFHIIWPPSAINYNFWPQNPPSYRAANEEYSKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE AGG+ELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERAGGEELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+G WYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRT VNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGQWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTRVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIP 335
           EVGPHPKLVDE+NPPKYKTKKYSEYAYCKLNKIP
Sbjct: 301 EVGPHPKLVDEKNPPKYKTKKYSEYAYCKLNKIP 334

[Close]

BLAST of Lsi08G004100 vs. NCBI nr
Match: XP_008445671.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa] >TYK11311.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 656.8 bits (1693), Expect = 1.0e-184
Identity = 307/335 (91.64%), Postives = 323/335 (96.42%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           M+VGRVQ++ASS+LFS  LPSEFVRPETEQPGITTVKGQVLAVPVIDF+NPD+DSVL AI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
            DAS NWGMFQIINH+IPNEAITHLQAVGKEFFELPPEEKE+YAKPP SKSVEGYGTKL+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHI+WP S+INYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWYDVNYIPNALVIHIGDQ+EILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENP KYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Lsi08G004100 vs. NCBI nr
Match: XP_004140862.1 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothetical protein Csa_005329 [Cucumis sativus])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 304/335 (90.75%), Postives = 321/335 (95.82%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQ++ASS+LFS  LPS+FVRP+TEQPGITTVKGQVLAVPVIDF+NPD+DSVL AI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
            DAS NWGMFQIINH+IPNEAITHLQAVGKEFF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHI+WP S+INYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWYDVNYIPNALVIHIGDQ+EILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVDE+ P +YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Lsi08G004100 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 648.7 bits (1672), Expect = 2.8e-182
Identity = 301/335 (89.85%), Postives = 320/335 (95.52%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIASS LFS +LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPDEDSV+RAI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
           DDASRNWGMFQIINH+IPNEAI HLQAVGK FFELPPEEKEVYAKPP SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGKKGWVDHLFHI+WP SAIN+ FWPQNPPSYRAANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWY+VNYIPNALVIHIGDQ+EILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Lsi08G004100 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 647.5 bits (1669), Expect = 6.3e-182
Identity = 300/335 (89.55%), Postives = 320/335 (95.52%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEVGRVQDIASS LFS +LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPDEDSV++AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
           DDASRNWGMFQIINH+IPNEAI HLQAVGK FFELPPEEKEVYAKPP SKSVEGYGTKLQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGKKGWVDHLFHI+WP SAIN+ FWPQNPPSYRAANEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE AGGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FR+GHWY+VNYIPNALVIHIGDQ+EILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NPPKYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Lsi08G004100 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 455.3 bits (1170), Expect = 4.3e-128
Identity = 207/334 (61.98%), Postives = 257/334 (76.95%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQDI+SS L ++ +P EF+R E EQP ITT +G   A+PV+D S+PDE+SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
             AS  WG+FQ++NH IP E I  LQ VG++FFELP  EKE  AKP  SK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           K+ +GKK WVDHLFH +WP S +NY FWP+NPP YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGL+   LKEG GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F++ HW+D  YIP+A+++HIGDQ+  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIP 335
            VGP P+L  ++NPPK+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Lsi08G004100 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 455.3 bits (1170), Expect = 4.3e-128
Identity = 207/334 (61.98%), Postives = 257/334 (76.95%), Query Frame = 0


Query: 1   MEVGRVQDIASSVLFSDNLPSEFVRPETEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAI 60
           MEV RVQDI+SS L ++ +P EF+R E EQP ITT +G   A+PV+D S+PDE+SV RA+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120
             AS  WG+FQ++NH IP E I  LQ VG++FFELP  EKE  AKP  SK +EGYGTKLQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180
           K+ +GKK WVDHLFH +WP S +NY FWP+NPP YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGL+   LKEG GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F++ HW+D  YIP+A+++HIGDQ+  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPPKYKTKKYSEYAYCKLNKIP 335
            VGP P+L  ++NPPK+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Lsi08G004100 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 342.4 bits (877), Expect = 4.0e-94
Identity = 157/294 (53.40%), Postives = 202/294 (68.71%), Query Frame = 0


Query: 41  LAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEK 100
           L +PVID SNPDE+ V  A+  AS+ WG+FQ++NH IP E I  L  VG EFFELP  EK
Sbjct: 12  LDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETEK 71

Query: 101 EVYAKPPGSKSVEGYGTKLQKEIDGKKGWVDHLFHIVWPSSAINYNFWPQNPPSYRAANE 160
           E  AKP  S  +EGY TK QK+++G+  WVDHLFH +WP S +N+ FWP+NPP Y   NE
Sbjct: 72  EAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVNE 131

Query: 161 EYRKHLHGVVEKLFRSLSLGLGLEGHELKEGAGGDELVYLLKINYYPPCPRPDLALGVPA 220
           EY  H+  + EK+   LS GLGL    LKEG GG+ + YL+KINYYPPCP P+L +G P 
Sbjct: 132 EYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAPD 191

Query: 221 HTDMSAITILVPNEVQGLQAFREGHWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTT 280
           HTD++ IT+LV NE  GLQAF++  W D  Y  + +++ IGDQ   +SNGKYKSV HR  
Sbjct: 192 HTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRAK 251

Query: 281 VNKEKTRISWPVFLEPPPEFEVGPHPKLV-DEENPPKYKTKKYSEYAYCKLNKI 334
           ++KEKTRISWPVF+E   +   GP P+L+  +EN PK+K   Y +Y + KL K+
Sbjct: 252 MDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

[Close]

BLAST of Lsi08G004100 vs. TAIR 10
Match: AT5G63600.1 (flavonol synthase 5 )

HSP 1 Score: 292.4 bits (747), Expect = 4.8e-79
Identity = 140/310 (45.16%), Postives = 198/310 (63.87%), Query Frame = 0


Query: 17  DNLPSEFVRPE-TEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIINH 76
           D+  SE   P  ++Q   +T+ G  + VPV+D S  DED ++R +  AS  WG+FQ++NH
Sbjct: 6   DHNASESSLPSLSKQLESSTLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNH 65

Query: 77  EIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQKEIDGKKGWVDHLFH 136
            IP E +  LQ VG +FFELP  EKE  AK    +  EGY    +  + G   W +HLFH
Sbjct: 66  GIPTELMRQLQMVGTQFFELPDAEKETVAK---EEDFEGY---KKNYLGGINNWDEHLFH 125

Query: 137 IVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKEGAGGD 196
            + P S INY +WP+NPP YR   EEY KH+  + EK+   LS GLGL+     +  GGD
Sbjct: 126 RLSPPSIINYKYWPKNPPQYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGD 185

Query: 197 ELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFREGHWYDVNYIPNA 256
              Y+L++N+YPP    +L +G  AH+DM AI +L+PNEV GLQAF++  W D++YI +A
Sbjct: 186 TAEYVLRVNFYPPTQDTELVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSA 245

Query: 257 LVIHIGDQMEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENPP 316
           +V+ IGDQ+  ++NG+ K+V HR   +K+K RISWPVF+ P  +  VGP P+   +ENPP
Sbjct: 246 VVVIIGDQLMRMTNGRLKNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPP 305

Query: 317 KYKTKKYSEY 326
           K++T  Y++Y
Sbjct: 306 KFETLIYNDY 309

[Close]

BLAST of Lsi08G004100 vs. TAIR 10
Match: AT5G63600.2 (flavonol synthase 5 )

HSP 1 Score: 290.0 bits (741), Expect = 2.4e-78
Identity = 140/311 (45.02%), Postives = 199/311 (63.99%), Query Frame = 0


Query: 17  DNLPSEFVRPE-TEQPGITTVKGQVLAVPVIDFSNPDEDSVLRAIDDASRNWGMFQIINH 76
           D+  SE   P  ++Q   +T+ G  + VPV+D S  DED ++R +  AS  WG+FQ++NH
Sbjct: 6   DHNASESSLPSLSKQLESSTLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNH 65

Query: 77  EIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQKEIDGKKGWVDHLFH 136
            IP E +  LQ VG +FFELP  EKE  AK    +  EGY    +  + G   W +HLFH
Sbjct: 66  GIPTELMRQLQMVGTQFFELPDAEKETVAK---EEDFEGY---KKNYLGGINNWDEHLFH 125

Query: 137 IVWPSSAINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLGLGLEGHELKEGAGGD 196
            + P S INY +WP+NPP YR   EEY KH+  + EK+   LS GLGL+     +  GGD
Sbjct: 126 RLSPPSIINYKYWPKNPPQYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGD 185

Query: 197 ELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFREGHWYDVNYIPNA 256
              Y+L++N+YPP    +L +G  AH+DM AI +L+PNEV GLQAF++  W D++YI +A
Sbjct: 186 TAEYVLRVNFYPPTQDTELVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSA 245

Query: 257 LVIHIGDQMEI-LSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENP 316
           +V+ IGDQ+ + ++NG+ K+V HR   +K+K RISWPVF+ P  +  VGP P+   +ENP
Sbjct: 246 VVVIIGDQLMVWMTNGRLKNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENP 305

Query: 317 PKYKTKKYSEY 326
           PK++T  Y++Y
Sbjct: 306 PKFETLIYNDY 310

The following BLAST results are available for this feature:

BLAST of Lsi08G004100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q07512	7.3e-149	72.02	Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ...	[more]
Q9M547	2.0e-146	71.04	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]
Q9XHG2	6.4e-145	74.14	Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2...	[more]
Q7XZQ6	8.3e-145	70.03	Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS...	[more]
Q41452	2.1e-140	68.93	Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]

BLAST of Lsi08G004100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CHN5	5.0e-185	91.64	Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BCS7	5.0e-185	91.64	flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC10348863...	[more]
A0A0A0K885	6.1e-183	90.75	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1FDW3	6.8e-182	89.25	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JY72	1.7e-180	88.36	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Lsi08G004100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885635.1	2.4e-189	95.21	flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida]	[more]
XP_008445671.1	1.0e-184	91.64	PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921....	[more]
XP_004140862.1	1.3e-182	90.75	flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothet...	[more]
XP_023551148.1	2.8e-182	89.85	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
KAG7016505.1	6.3e-182	89.55	Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Lsi08G004100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08640.1	4.3e-128	61.98	flavonol synthase 1	[more]
AT5G08640.2	4.3e-128	61.98	flavonol synthase 1	[more]
AT5G63590.1	4.0e-94	53.40	flavonol synthase 3	[more]
AT5G63600.1	4.8e-79	45.16	flavonol synthase 5	[more]
AT5G63600.2	2.4e-78	45.02	flavonol synthase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..333 e-value: 1.2E-98 score: 332.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 43..150 e-value: 3.4E-24 score: 85.9
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 201..296 e-value: 1.6E-25 score: 89.5
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 6..332
None	No IPR available	PANTHER	PTHR47990:SF36	OS02G0767300 PROTEIN	coord: 6..332
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 16..333
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 196..296 score: 14.434731

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G004100.1	Lsi08G004100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051555	flavonol biosynthetic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0045431	flavonol synthase activity
molecular_function	GO:0046872	metal ion binding