Lsi08G003990 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G003990 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.6e-11
Identity = 51/174 (29.31%), Postives = 89/174 (51.15%), Query Frame = 0

Query: 10  GRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVIKD 69
           G ++ + K +  L LA+SR+ IL  ++         +V +LL+  +   A +RVE +I+D
Sbjct: 5   GPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRD 64

Query: 70  QNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIKSV 129
           +  ++ + +IE    LL  R  L+   +E P E+KE++  L++++ R    PEL +IK+ 
Sbjct: 65  EYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQ 124

Query: 130 LTTRFGKEFTARAVELRNNC----GVNHLLMQKLSTRQPTLENRMEVLKAIASE 180
           L  ++GK      +E   NC     VN  ++ KLS   P      + L  IA +
Sbjct: 125 LKAKYGK-----GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEK 172

BLAST of Lsi08G003990 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-10
Identity = 47/158 (29.75%), Postives = 79/158 (50.00%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Lsi08G003990 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-10
Identity = 47/158 (29.75%), Postives = 79/158 (50.00%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Lsi08G003990 vs. ExPASy Swiss-Prot
Match: Q568Z6 (IST1 homolog OS=Rattus norvegicus OX=10116 GN=Ist1 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.1e-10
Identity = 47/158 (29.75%), Postives = 79/158 (50.00%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVI 67
           +LG  F+A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Lsi08G003990 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 5.3e-10
Identity = 46/158 (29.11%), Postives = 78/158 (49.37%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVI 67
           +LG   +A + R  L L ++RL +L  ++     +A  ++   L      RA +RVE +I
Sbjct: 1   MLGSGIKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHII 60

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRC-GDFPELHEI 127
           ++   ++A  ++E Y +LLL R  L++   E    L E+V+ L++AA R   +  EL  +
Sbjct: 61  REDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIV 120

Query: 128 KSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQP 165
              L  ++ KE+  +         VN  LM KLS   P
Sbjct: 121 ADQLCAKYSKEY-GKLCRTNQIGTVNDRLMHKLSVEAP 157

BLAST of Lsi08G003990 vs. ExPASy TrEMBL
Match: A0A0A0KAU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G032470 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 8.7e-133
Identity = 290/389 (74.55%), Postives = 313/389 (80.46%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDALLGRNFRASKFRPLLNL+LSRLSILT QRR+ CSQA+SDVLQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVN  LMQKLSTRQPTLE RM+ LK+IASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GIVL+IDQ   S + K+ RN RQ++AE Q E     +FS EVASGSK  YKDVADAAQAA
Sbjct: 181 GIVLQIDQLPSSKQEKVGRNGRQSEAEGQSE-----EFSNEVASGSKTTYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHDGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIRYRNR 300
           FESAAQAAAAARAAMELSRSH+GPSSP+  GSGT+  NKQ+ EK EVESK K E+     
Sbjct: 241 FESAAQAAAAARAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEM----- 300

Query: 301 REGDEEEGSKKRGEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLDIETK-- 360
               EE G+ ++GE                 E +E+E   MDEER S   NGL +ETK  
Sbjct: 301 ----EEYGNGRKGE----------------GEGEEEEGDGMDEERTS---NGLKMETKVE 356

Query: 361 ---VSEKASFRLNLEKKPISVRTRRVRGY 385
              VSEK SFRLNLEKKPISVRTRRV GY
Sbjct: 361 KTEVSEKGSFRLNLEKKPISVRTRRVGGY 356

BLAST of Lsi08G003990 vs. ExPASy TrEMBL
Match: A0A6J1E0Z0 (uncharacterized protein LOC111429814 OS=Cucurbita moschata OX=3662 GN=LOC111429814 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 8.7e-133
Identity = 290/395 (73.42%), Postives = 322/395 (81.52%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDALLGRNFRASKFRPLLNLALSRL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTR P LE+RM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GI L++D+   SNE    RN RQ Q+E    V ENL+FSTEV SGSKQKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIR 300
           FESAAQAAAAARAAMELSRS     DGPSSP   GS T+ ++KQE +K+EVE+KPK EI 
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSPGP-GSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 YRNRREGDEEEGSKKR--GEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLD 360
           Y     G EEEGSK R   EVKNS+ G     C    E   KE  EMDE+RA+N++ G +
Sbjct: 301 Y---EYGKEEEGSKIRAEAEVKNSMCG-----CSSNRELFVKENREMDEQRATNIEIGEE 360

Query: 361 IE-----TKVSEKASFRLNLEKKPISVRTRRVRGY 385
           +E     T+++EK SFRLNLEKKPISVRTRRVRGY
Sbjct: 361 MEANLEKTEITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of Lsi08G003990 vs. ExPASy TrEMBL
Match: A0A6J1I2V3 (uncharacterized protein LOC111470447 OS=Cucurbita maxima OX=3661 GN=LOC111470447 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 8.1e-131
Identity = 288/395 (72.91%), Postives = 319/395 (80.76%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDALLGRNFRASKFRPLLNLA+SRL+ILT QRRLR +QA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRTQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVL+EGYLNLL+ERT+LLEQ+ ECPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLMEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLTT FGKEFTARAVELRNNCGVNHLLMQKLSTRQP LE+RM++LK IASEN
Sbjct: 121 PELHEIKSVLTTHFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GI L++D+   SNE    RN RQ Q+E    V ENL+ STEV SGSKQKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKLSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIR 300
           FESAAQAAAAARAAMELSRS     DGPSSP   GS TS ++KQE +K+ VE+KPK EI 
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSPGP-GSATSSYDKQEQKKSNVEAKPKLEIE 300

Query: 301 YRNRREGDEEEGSKKR--GEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLD 360
           Y     G EEEGSK R   EVKNS+ G     C    E   KE  EMDE+RASN++ G +
Sbjct: 301 Y---EYGREEEGSKIRPKPEVKNSMCG-----CSSNRELFVKENREMDEQRASNIEIGEE 360

Query: 361 IE-----TKVSEKASFRLNLEKKPISVRTRRVRGY 385
           +E     T ++EK SFRLNLEKKPISVRTRRVRGY
Sbjct: 361 MEANLEKTAITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of Lsi08G003990 vs. ExPASy TrEMBL
Match: A0A6J1CFG6 (uncharacterized protein LOC111010325 OS=Momordica charantia OX=3673 GN=LOC111010325 PE=3 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 3.4e-129
Identity = 283/399 (70.93%), Postives = 322/399 (80.70%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDALLGRNFRASKFRPLLNLALSRL++LT QR +R SQA SD LQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLAVLTNQRHVRRSQARSDALQLLQLGHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIK+QNALDAYVLIEGYLNLL+ERT LLEQ+ ECPEELKEAV+GL+FAASRCGDF
Sbjct: 61  LRVEQVIKEQNALDAYVLIEGYLNLLIERTDLLEQERECPEELKEAVSGLIFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PEL EIKSVLTTRFGKEFTARAVELRNNCGV+H +MQKLSTRQP LE+RM VL+AIASEN
Sbjct: 121 PELQEIKSVLTTRFGKEFTARAVELRNNCGVDHSIMQKLSTRQPNLESRMNVLEAIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQT-QAEAQIEVAENLQFSTEVASGS---KQKYKDVADA 240
            IVL++D+   SNE K  RN RQ  Q E + EV +NLQFS +V SGS   KQKYKDVADA
Sbjct: 181 NIVLQLDEHPLSNEEKFARNRRQNYQQEPKAEVGDNLQFSADVPSGSMQAKQKYKDVADA 240

Query: 241 AQAAFESAAQAAAAARAAMELSRSH----DGPSSPNSHGSGTSPHNKQEAEKAEVESKPK 300
           AQAAFESAAQAAAAARAAMELSRS     D PS+P   GSG S + +QE +K+EVESKPK
Sbjct: 241 AQAAFESAAQAAAAARAAMELSRSQSQDPDDPSTPKP-GSG-STNKRQEQKKSEVESKPK 300

Query: 301 PEIRYRNRREGDEEEGSKKRGEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNG 360
            EI Y+NRRE +EE GSK   EVKNS+    SPS         +E SEM+E+RA N++ G
Sbjct: 301 QEIEYQNRRE-EEEAGSKITAEVKNSMPASFSPS---------REDSEMEEQRAPNVETG 360

Query: 361 LDIETKV-------SEKASFRLNLEKKPISVRTRRVRGY 385
           LD+ET+        ++K SF LNLEKKP+SVRTRRVRG+
Sbjct: 361 LDMETETKPELTEETQKPSFGLNLEKKPMSVRTRRVRGF 387

BLAST of Lsi08G003990 vs. ExPASy TrEMBL
Match: A0A6J1E4E5 (uncharacterized protein LOC111429823 OS=Cucurbita moschata OX=3662 GN=LOC111429823 PE=3 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 3.9e-125
Identity = 278/395 (70.38%), Postives = 312/395 (78.99%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDALLGRNFRASKFRPLLNL LSRL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLTLSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVLIEGYLNLL+ER +LLEQ+ ECPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERASLLEQERECPEELKEALAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKS LT+RFGKEFTARAVELRNNCGVNHLLMQKLSTR P LE+RM++LK IASEN
Sbjct: 121 PELHEIKSALTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GI L++D+   SNE    RN RQ Q E    V ENL+FSTEV SGSKQKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQPETDGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIR 300
           FESAAQAAAAARAAMELSR      DGPSSP   GS T+ ++KQ  ++++VE+KPK EI 
Sbjct: 241 FESAAQAAAAARAAMELSRPESQDPDGPSSPGP-GSATTSYHKQVQKESDVEAKPKLEIE 300

Query: 301 YRNRREGDEEEGSK--KRGEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLD 360
           Y     G EEEGSK   + EVKNS+ G     C    E   KE  EMDE+RA+N++ G +
Sbjct: 301 Y---EYGREEEGSKVTPKPEVKNSMCG-----CSSNRELFVKENREMDEQRATNIEIGEE 360

Query: 361 IE-----TKVSEKASFRLNLEKKPISVRTRRVRGY 385
           +E      +++EK SF LNLEKKPI VRT RVRGY
Sbjct: 361 MEANLEKAEITEKPSFGLNLEKKPILVRTGRVRGY 383

BLAST of Lsi08G003990 vs. NCBI nr
Match: XP_038884116.1 (uncharacterized protein LOC120075040 [Benincasa hispida])

HSP 1 Score: 559.3 bits (1440), Expect = 2.6e-155
Identity = 319/396 (80.56%), Postives = 336/396 (84.85%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDALLGRNFRASKFRPLLNLALSRLSILT QRR+RCSQA+SDVLQLLQLP+HHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLALSRLSILTAQRRVRCSQANSDVLQLLQLPNHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLE+RMEVLKAIASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLESRMEVLKAIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GIVL+ID   PSNE KLTRN RQ Q E + EV ENLQFSTEVASGSKQKYKDVADAAQAA
Sbjct: 181 GIVLQIDNHPPSNEEKLTRNTRQNQPEPRSEVVENLQFSTEVASGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHDGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIRYRNR 300
           FESAAQAAAAARAAMELSRSHDGPSSP S GSGT+PH+KQE EKAEVESKP PE   RNR
Sbjct: 241 FESAAQAAAAARAAMELSRSHDGPSSPKSPGSGTTPHDKQELEKAEVESKPNPETENRNR 300

Query: 301 REG-------DEEEGSKKRGEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGL 360
           R G       +EEEG+K RG+                         EMDEER S +KNGL
Sbjct: 301 RGGGGGEEEEEEEEGNKMRGD-------------------------EMDEERGSKVKNGL 360

Query: 361 DIE-----TKVSEKASFRLNLEKKPISVRTRRVRGY 385
           ++E     T+VSEK SFRLNLEKKPISVRTRRVRG+
Sbjct: 361 NVERKVEKTEVSEKGSFRLNLEKKPISVRTRRVRGH 371

BLAST of Lsi08G003990 vs. NCBI nr
Match: XP_011656519.1 (uncharacterized protein LOC101211044 [Cucumis sativus] >KGN45949.1 hypothetical protein Csa_005294 [Cucumis sativus])

HSP 1 Score: 483.4 bits (1243), Expect = 1.8e-132
Identity = 290/389 (74.55%), Postives = 313/389 (80.46%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+KLDALLGRNFRASKFRPLLNL+LSRLSILT QRR+ CSQA+SDVLQLLQL HHHRAL
Sbjct: 1   MGRKLDALLGRNFRASKFRPLLNLSLSRLSILTAQRRVGCSQANSDVLQLLQLSHHHRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF
Sbjct: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLTTRFGKEFTARAVELRNNCGVN  LMQKLSTRQPTLE RM+ LK+IASEN
Sbjct: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNLSLMQKLSTRQPTLETRMDALKSIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GIVL+IDQ   S + K+ RN RQ++AE Q E     +FS EVASGSK  YKDVADAAQAA
Sbjct: 181 GIVLQIDQLPSSKQEKVGRNGRQSEAEGQSE-----EFSNEVASGSKTTYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSHDGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIRYRNR 300
           FESAAQAAAAARAAMELSRSH+GPSSP+  GSGT+  NKQ+ EK EVESK K E+     
Sbjct: 241 FESAAQAAAAARAAMELSRSHEGPSSPSKPGSGTTSDNKQKKEKREVESKVKQEM----- 300

Query: 301 REGDEEEGSKKRGEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLDIETK-- 360
               EE G+ ++GE                 E +E+E   MDEER S   NGL +ETK  
Sbjct: 301 ----EEYGNGRKGE----------------GEGEEEEGDGMDEERTS---NGLKMETKVE 356

Query: 361 ---VSEKASFRLNLEKKPISVRTRRVRGY 385
              VSEK SFRLNLEKKPISVRTRRV GY
Sbjct: 361 KTEVSEKGSFRLNLEKKPISVRTRRVGGY 356

BLAST of Lsi08G003990 vs. NCBI nr
Match: XP_022921602.1 (uncharacterized protein LOC111429814 [Cucurbita moschata] >KAG7032508.1 IST1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 483.4 bits (1243), Expect = 1.8e-132
Identity = 290/395 (73.42%), Postives = 322/395 (81.52%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDALLGRNFRASKFRPLLNLALSRL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVLIEGYLNLL+ERT+LLEQQ +CPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQQRDCPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTR P LE+RM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GI L++D+   SNE    RN RQ Q+E    V ENL+FSTEV SGSKQKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIR 300
           FESAAQAAAAARAAMELSRS     DGPSSP   GS T+ ++KQE +K+EVE+KPK EI 
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSPGP-GSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 YRNRREGDEEEGSKKR--GEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLD 360
           Y     G EEEGSK R   EVKNS+ G     C    E   KE  EMDE+RA+N++ G +
Sbjct: 301 Y---EYGKEEEGSKIRAEAEVKNSMCG-----CSSNRELFVKENREMDEQRATNIEIGEE 360

Query: 361 IE-----TKVSEKASFRLNLEKKPISVRTRRVRGY 385
           +E     T+++EK SFRLNLEKKPISVRTRRVRGY
Sbjct: 361 MEANLEKTEITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of Lsi08G003990 vs. NCBI nr
Match: KAG6601794.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.6 bits (1233), Expect = 2.6e-131
Identity = 288/395 (72.91%), Postives = 321/395 (81.27%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDALLGRNFRASKFRPLLNLA+SRL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLAVSRLAILTNQRRLRRSQAQSDVLQLLQLHHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVLIEGYLNLL+ER +LLEQ+ ECPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERASLLEQERECPEELKEALAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTR P LE+RM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRHPNLESRMDLLKTIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GI L++D+   SNE    RN RQ Q+E    V ENL+FSTEV SGSKQKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSRQNQSEPHGGVGENLKFSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIR 300
           FESAAQAAAAARAAMELSRS     DGPSSP   GS T+ ++KQE +K+EVE+KPK EI 
Sbjct: 241 FESAAQAAAAARAAMELSRSESQDPDGPSSPGP-GSATTSYHKQEQKKSEVEAKPKSEIE 300

Query: 301 YRNRREGDEEEGSKKR--GEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLD 360
           Y     G EEEGSK R   EVKNS+ G     C    E   KE  EMDE+RA+N++ G +
Sbjct: 301 Y---EYGKEEEGSKIRAEAEVKNSMCG-----CSSNRELFVKENREMDEQRATNIEIGEE 360

Query: 361 IE-----TKVSEKASFRLNLEKKPISVRTRRVRGY 385
           +E     T+++EK SFRLNLEKKPISVRTRRVRGY
Sbjct: 361 MEANLEKTEITEKPSFRLNLEKKPISVRTRRVRGY 383

BLAST of Lsi08G003990 vs. NCBI nr
Match: XP_023538782.1 (uncharacterized protein LOC111799606 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477.2 bits (1227), Expect = 1.3e-130
Identity = 288/395 (72.91%), Postives = 320/395 (81.01%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MG+ LDALLGRNFRASKFRPLLNLALSRL+ILT QRRLR SQA SDVLQLLQL H  RAL
Sbjct: 1   MGRTLDALLGRNFRASKFRPLLNLALSRLAILTNQRRLRRSQAQSDVLQLLQLRHQQRAL 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
           LRVE+VIKDQNALDAYVLIEGYLNLL+ERT+LLEQ+ ECPEELKEA+AGLLFAASRCGDF
Sbjct: 61  LRVEQVIKDQNALDAYVLIEGYLNLLIERTSLLEQERECPEELKEAIAGLLFAASRCGDF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PELHEIKSVLT+RFGKEFTARAVELRNNCGVNHLLMQKLSTRQP LE+RM++LK IASEN
Sbjct: 121 PELHEIKSVLTSRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPNLESRMDLLKTIASEN 180

Query: 181 GIVLEIDQPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAA 240
           GI L++D+   SNE    RN  Q Q E    V ENL+ STEV SGSKQKYKDVADAAQAA
Sbjct: 181 GITLQLDETPLSNE---ARNSSQNQPEPHGVVGENLKLSTEVPSGSKQKYKDVADAAQAA 240

Query: 241 FESAAQAAAAARAAMELSRSH----DGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIR 300
           FESAAQAAAAARAAMELSR      DGPSSP   GSGT+ ++KQE +K++VE+KPK EI 
Sbjct: 241 FESAAQAAAAARAAMELSRPESQDPDGPSSPGP-GSGTTSYDKQEQKKSDVEAKPKLEIE 300

Query: 301 YRNRREGDEEEGSKKR--GEVKNSVAGDSSPSCELCDEEKEKEKSEMDEERASNLKNGLD 360
           Y     G EEEGSK R   EVKNS+ G     C    E   KE  EMDE+RASN++ G +
Sbjct: 301 Y---EYGREEEGSKIRAEAEVKNSMCG-----CSSNRELFVKENREMDEQRASNIEIGEE 360

Query: 361 IE-----TKVSEKASFRLNLEKKPISVRTRRVRGY 385
           +E     T+++EK SFRLNLEKKPISVRTRRVRG+
Sbjct: 361 MEANLEKTEITEKPSFRLNLEKKPISVRTRRVRGH 383

BLAST of Lsi08G003990 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.3e-69
Identity = 189/411 (45.99%), Postives = 253/411 (61.56%), Query Frame = 0

Query: 1   MGKKLDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRAL 60
           MGKKLDALLGR+F+ +KF+ L+ LAL+RLSIL  QR+ R SQA SDV +LL+L  H  A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  LRVEKVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDF 120
            RV++V+KDQN LD    I GY  L L+R  L E   +CPEEL EAV+GLLFAASR G+F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELHEIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASEN 180
           PEL EI++VL +RFGK+  AR++ELR+NCGV+  ++QKLSTR P  E RM+ LK IA+EN
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

Query: 181 GIVLEIDQPSPSNEGKL----TRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADA 240
            IVL++DQ S S EG      T +V +T+  ++    E    S  V  G K+KYKDVADA
Sbjct: 181 NIVLKLDQASTSTEGTTNMQGTSDVSKTKLTSKDGRGEGYGLSDSVKRG-KKKYKDVADA 240

Query: 241 AQAAFESAAQAAAAARAAMELS----RSHDGPSS---PNSHGSGTSPHNKQEAEKAEVES 300
           AQAAFESAA AA AA+AA+ELS    R HD P +    NS     +  ++QE E  +  S
Sbjct: 241 AQAAFESAAHAAEAAQAAVELSQFSPRGHDSPGNIGGENSFHGSENKESEQEQEGNDDSS 300

Query: 301 KPKPEIRYRNRREGDEEEG-------SKKRGEV----KNSVAGDS----SPSCELCDEEK 360
           + + ++   ++R   + E        S +   V    K+++  DS     PS E     K
Sbjct: 301 EGEGDVMSESKRSMSDSEDIIDVPVMSFREDPVKLLEKDTIIYDSEEETQPSVETITISK 360

Query: 361 EK-EKSEMDEERASNLKNGLDIETKVSEKASFRLNLEKKPISVRTRRVRGY 385
            K E++ M     ++ ++ +D   +  E    R    K P+SVRTR+VRGY
Sbjct: 361 SKDEQNRMVGSNRADTRH-VDHMVRSIEDPIMRKAGLKGPVSVRTRQVRGY 409

BLAST of Lsi08G003990 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 126.3 bits (316), Expect = 5.2e-29
Identity = 96/314 (30.57%), Postives = 156/314 (49.68%), Query Frame = 0

Query: 5   LDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVE 64
           L+ L  R    +K +  LNLA++R+ +L  +R ++      ++   LQ      A +RVE
Sbjct: 4   LNQLFNRGIFGAKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVE 63

Query: 65  KVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELH 124
            VI++ N   AY ++E +   +L R  +LE + ECP EL+EA+A ++FAA RC + P+L 
Sbjct: 64  HVIREMNLWAAYEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLL 123

Query: 125 EIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASENGIVL 184
           +IK++  T++GKEF   A ELR + GVN  +++KLS   P+   R+++LK IA E  +  
Sbjct: 124 QIKNLFGTKYGKEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQEYSLNW 183

Query: 185 E---------------------------IDQPSPSNEG----KLTRNVRQTQAE------ 244
           +                           I +  PS +G     ++R V    AE      
Sbjct: 184 DSSATEAEFMKSHEDLLGGAKQIHRQDGISESRPSQQGYGQSSVSREVESLPAEATQRFQ 243

Query: 245 ---AQIEVAENLQFST-----EVASGSKQKYKDVADAAQAAFESAAQAAAAARAAMELSR 274
              AQ  V++++  S      +    +++   DV + A+AA  SA +A AAARAA +L  
Sbjct: 244 KLQAQNPVSKSMPSSKLTSAFQAPPDTRRNQTDVMEIARAALASADRATAAARAAAQLVN 303

BLAST of Lsi08G003990 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 122.5 bits (306), Expect = 7.5e-28
Identity = 104/358 (29.05%), Postives = 170/358 (47.49%), Query Frame = 0

Query: 7   ALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKV 66
           +L  R F +SK +    +A++R+ ++  +R +   Q   D+  LLQ      A +RVE V
Sbjct: 20  SLFRRGFNSSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHV 79

Query: 67  IKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEI 126
           I++QN   A  +IE +  L++ R T++ +Q +CP +LKE +A L+FAA RC + PEL ++
Sbjct: 80  IREQNIQAANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDL 139

Query: 127 KSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASENGIVLEI 186
           + +   ++GK+F + A +LR +CGVN +L+ KLS R P  E +++++K IA E     ++
Sbjct: 140 RDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKE----FQV 199

Query: 187 DQPSPSNEGKLTR------------------NVRQTQAEAQIEVAENLQFSTEVASGSKQ 246
           D  +   E +L +                   V +      I+  + +  ST   S    
Sbjct: 200 DWDTTETEQELLKPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMS-INT 259

Query: 247 KYKDVADAAQAAFESAAQAAAAARAAMELSRSHDGPSSPNSHGSGTSPHNKQEAEKAEVE 306
            Y D   AA+AA E A QA AAA+ A  L+   D  +   S  S  S H K         
Sbjct: 260 HYHDTESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHH 319

Query: 307 SKPKPEIRYRN-------RREGDEEEGSKKRGEVKNSVAGDSSPSCELCDEEKEKEKS 340
             P    + R+        + G E  G  +R    N    +S    E  + E E +++
Sbjct: 320 HHPGSRRQSRDSETSSYYAKPGAENRGMGRRHSYNNPGINESDYEEEYTNTEAEAKET 372

BLAST of Lsi08G003990 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 113.6 bits (283), Expect = 3.5e-25
Identity = 89/317 (28.08%), Postives = 157/317 (49.53%), Query Frame = 0

Query: 5   LDALLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVE 64
           LD+   + F+A+K + LL L + R+ ++  +R  +  Q   ++ +LL+      A +RVE
Sbjct: 4   LDSFFNKGFKAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVE 63

Query: 65  KVIKDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELH 124
            +I+++  + A  ++E +  L+  R  ++E Q ECP +LKEA++ + FAA RC D  EL 
Sbjct: 64  HIIREEKMMAAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQ 123

Query: 125 EIKSVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASENGIVL 184
           +++ +  +++GKEF A A EL+ + GVN  L++ LS R P+ E ++++LK IA E+    
Sbjct: 124 QVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEEH---- 183

Query: 185 EIDQPSPSNEGKLTRN-------VRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAA 244
           E+D    S E  L ++        +Q    +++ + E     T + S S  K K  +D+ 
Sbjct: 184 ELDWDPASTETDLFKSHEDLLDGPKQFGGGSKLPLPEEQNEKTNLTSLSAAKEKSDSDSE 243

Query: 245 QAAFE---------------SAAQAAAAARAAMELSRSHDGPSSPNSHGSGTSPHNKQE- 296
               +               ++  A  AA++A     SHD P    + G   +   + E 
Sbjct: 244 YDILDFPEVPNVLLRPTPGATSVNAPDAAKSASYEHTSHDLPFDSENAGVEKTASKRDEH 303

BLAST of Lsi08G003990 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 109.4 bits (272), Expect = 6.6e-24
Identity = 106/373 (28.42%), Postives = 177/373 (47.45%), Query Frame = 0

Query: 8   LLGRNFRASKFRPLLNLALSRLSILTTQRRLRCSQAHSDVLQLLQLPHHHRALLRVEKVI 67
           +L R F+ +K +  L +A SRL IL  ++ ++  Q   ++ QLL+      A +RVE V+
Sbjct: 4   VLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVV 63

Query: 68  KDQNALDAYVLIEGYLNLLLERTTLLEQQSECPEELKEAVAGLLFAASRCGDFPELHEIK 127
           +++  + AY LI  Y  LL+ R  ++E Q  CP +LKEAV  +LFA+ R  D PEL EI 
Sbjct: 64  REEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIF 123

Query: 128 SVLTTRFGKEFTARAVELRNNCGVNHLLMQKLSTRQPTLENRMEVLKAIASENGIVLEID 187
              TT++GK+F+  AVELR + GV+ LL++KLS + P    ++++L AIA E+ +V E  
Sbjct: 124 KQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVWEAQ 183

Query: 188 QPSPSNEGKLTRNVRQTQAEAQIEVAENLQFSTEVASGSKQKYKDVADAAQAAFESAAQA 247
               S+     ++          + A ++   + + S  +Q     A A   A   +++ 
Sbjct: 184 SFVESD----PKDTELLNGANSFQPASSMNMDSSINSNKEQPPNIHAPATVNAHHGSSE- 243

Query: 248 AAAARAAMELSRSHDGPSSPNSHGSGTSPHNKQEAEKAEVESKPKPEIRYRNRREGDEEE 307
                 + E S ++ G SS  S+   +   +     KA        E   RN   G E  
Sbjct: 244 ---RHHSPENSYANGGRSSSRSNNVTSGKADDYYHSKARPSRSRPDEGECRNPNHGYENS 303

Query: 308 GSKKRGEVKNSVAGDSSPSCELCDEEKEKEK-SEMDEERASNLKNGLDIETKVSEKASFR 367
            S+ + + +     DS+ +     E  E+   +       SN +     ++  S  +S  
Sbjct: 304 SSRNKQKWEPEFV-DSTDAARAAAEAAERASFAARAAAELSNKERMTRQDSTQSHISSAS 363

Query: 368 LNLEKKPISVRTR 380
           +NL  +P   R R
Sbjct: 364 VNLRNEPSHRRDR 367