Lsi08G003970 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G003970
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Regulator of Vps4 activity in the MVB pathway protein
Location	chr08: 10984757 .. 10990568 (-)
RNA-Seq Expression	Lsi08G003970
Synteny	Lsi08G003970

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGTGTTCAGATTAGATCAGAATTCAGATAATCTTGTTGTTGTTGAGCTTATAATTATAATTTTCTCCTTCTAATTTCTCTCTTGAGCTATCCGGAGAAAAAGAAAAGAAAAAAAAAAGAAAAGAATGAGAAGAATTGTGAAACGTGTTGTGGGAAGAAGTTTCAAGTCCTGTGGTTTCAGACGTCTGGTCCAATCTGCTTTCACGCGCCTCCAATCGCTGAAGAACCATCGCCATGGAAGATATTCCGAAGATGGAAACGACGCCGTTCGCCTCCTTCAAGATGGCCTTCCCAAACTTGCTCTCTCTCGCGTACGCTTCTCAGCTTCTTTTTTTCCTTTTTTTTTTTTTTTCCTCTTGTTTCATACTAATATTTCATTTCTCGGGCATTAAATAAATTGTAGGTTTAGTATTTATTTTTTTTTTTTACTTATAAATTGGATATATAATATTTATAAATAAATAAAATAATAATAAACTAGTCATAGTTGACCTTGTCAAACCTCTCAACAATCTTATGAAGGATGAAGGCTAGAGATTTCAGTGATGGCGATTATAAAAGGCTTGTGCTTACTACTAGAGATGTTCATGGATAGGGATGGCCCGTCTCCGTCCCTATGTAGCTTGCAGGAAATTTTCTTCTTCCACTACTTTTTTATGTGTGTGTAAATTTGTTTTTTAAATATAGAAAAATAAACAGGGGCGAATTTCCCGTCTCCATCCTCATGTAGCTCACGAAAAAAATTCCTTCCCCCATTCTCTATCTAAATAGGGATTCTCTGTTTCATTATTGTGGATATGGAGGACAAAGCTCGAGGCCGATAAGAAATAAAAAAGAGCAACTAACCAATAATCAAAATATGGAAGTATTCCATAAAATTTAAGGCAAAATACACGTTTGGTCATGAGAATATTTGAGGTAAGTGTCTATTTGGTTAGTGAGGTTTTATAATAGACACTTTTAGTCCTGGAAACTTGAATAATGGTTCTTGACTTAACAGCTGACTAGGGCATTAAATATTAAGGTGGACTTGTTTATTCAATTTTTACTCTAAAATTAAATTTATTAAAAATTTATTAGGTTGTTAAAAAGGAGAGTTTAAATACATAAATAAAGAAGAAAGAGGGAAATTTGAGAATGTGGCGGTGATTGTGAATCTGAGGTACCTGAAATGGAAGAAGTGAAAAGCAGTGAGAGGAAGAAGAGAAGAAGCATTGCGAAGTTGCAGAAGGATCAATCGGAGGGCATAATTCCGGCGAGAGAGAGAGAAAGAGAGAGAAGGTTTTTCTTCTTCTCCGATGAGAAGGCAAAGACAGGCTGTTCAATTGAATTTCTGCTTAGAATATGTTTTTGTATTTTGTACTTTTTTTTTTTAATTCCAGTGAAGAAATAAATAATGAAAAGGAATTTAGCACAATAATCATTTCTTTTAGCATAATAAGCCAATCTACCTCAACACTTAATAGTCCAATCTGCAATCTGTTAATTCAACTGGTTAAATAGATTTAGGGACCATTTAGAACCATTATCCAAATTTCGGGACTAAACGTGCCCATTATTAAATCTCATGGGCCAAATAGATACATACTCCAAATCTCAGGAACTTAAAATTTAATTCAAACACTCTAAATCAAGTCTAAACTACAAAAAGAGAGTATCCAAAAGTTAGAGACCGATCTAAATTACTTTAACTAAGTTCGAAAAGGCTTGAAGAAATTTGAATTACCTAAAACTCACCGAAACGAACATGGGTACTCACTAAAAACTGTTCAATTTGGTTCGATATCAATCTGAAGTTACAAATAGAAAAGTTGTAAACATTAGAAACTAAACTACAACTTTTAATTGAATTTTGAAAGGGTTTAAGGATGGTTATTACCATTGAAAGCTCACCAAAATGGTTCGAACGCTTTTTGGTTCGTGTTCGAATAAGCTTAAACCTATGGATTTAAAGTTGTTAAGGTGAGTGAATGCTAATCTAGGCCAAATCTAGAAGGGGTAAGGAAAAATCGTTGTACACTTGGTGAAAGTGATGAAAACGTTTAGGTGCAAATAAGCTTTGTAATTAAACTAATAGCTTATGCATCAAGTAGAATCTTATTCTAAAATTACATTAATCATTAATGAAAAAAGTACCATAGAAGTTTAGAACTTGAAGAAAAAAAATTAAAGCCCTCTAGTGAATCTACTATAATTTTCTTCAACAATTTTATTTTTTTTAAAAAAAAAAACAAGTACACATCATAAAGCAAACACATGGGACTTTGAAATATATGTGAAAACCTTAGGTAATGCTGGTTGAAACAAAAACTTAATCGAAGGTGTGAATTGATACAATTTATCTAATTTTAAAATTTAAATTAATTAAATCAAAAGTTTAGGGTTAAAATTGATATCACTTTATTGAGTGATATTATTGAGAATAGTCTTTTTAAACAAATTTTCTACCTAAATTATATTTTTATTACTTAAATACTAAAAATAAATTTAAAAAATCCGTCCAAGTAATCATTTATTTTTAATTTTTAATTATTAAAAAATAATTTTGTGCAGAAATAAATCAAAACTACAATATTTTCTACACCTGCCACTAATACACACACATCAACTTTCAAAATGTTTGAAGTATAAATTCCAAGTTTTGTTTTTAAAATAAAAAAAATTGGGTTTTCAAATATAAAATATTGTCTATCAAGTATAGAATAATTTTATTGTCTATCAACGATAAACTGTGGTCGAGTCTATCACAGTCTATCACGGATAGACAGTAAAAATTTTCTATACTGGTAAATAGTTTTGATATTTTTTTCATTTATAATAATTTTCTTTTTTAGGAGACTTTTCACAAATAGAAAAAAAAAACAAAAGTAATTACACATATAGAAAAAAAAAAGTGAGAAATACAAAAGTATGGGTACATTTTTTTCTATATGTGTAATTACTTTTATTTTTTTTCAATACACGACAATTTCCCCTTTTTTTTTTTTTTTTGATGTTTGCAGTGCGAGCAAATGATTAAACATCAAAATCTTTTGGATGCTTATGGAATGATTGAAGGATATCTCAATCTCTTGATGGAAAGGATCAACCTCCTTCGACGTGAAAGGTTTTGTTTGCATTATTATTTTATAAATCATTTTCATAATCAAAATTTTCATTTTCAAAAACATTATGTAGTCAGAAAGCTGTTTTTTTTTTTTTTCCATTTTTATCCCATATTTTGTGAATAATGATTTTAATTTCGGAAAATTTTTAAAAGTAGAAAAATAGAATAAACTATTTACACAAAATAGTAAAATTTTTAGATAATTGTGATAAACGCTGATAGAAATCTATCAAGATCTATAGTGATAGAAATAATAGAAGTCTATCACTGATACATGCTGATAGAAGTATATCAATATCTATCCGTTTTTTTTTTTTTTTTTGTTATTTTCTGTAAATAGTTTGACATTTTTTCTATATATGAAAATTTTCCTTTATTTTTCACGATAGTTTCATTAATCGATTATATTGTTTAAAACCAAATTGAAAAAAAAAACTATTTTTAAAACAAAATTAAATTATAGCTTTCTATCATAAAAATCAAATATTTGAATTTTCATATTTGCTTGACAAAAGAAAAAAAAAAGGGGTTAATTTGGGTTTTTTTAAAACTTTTATTTTGACAAAGGTTTTATGATTTTATGAAGAGAATGCCCTGATGAATTAAAGGAGGCAGTGTCAAGTGTAGTATTTGCAGCTTCAAGATGGAAGGATTTTCCCGAACTTGAAGACATTAAATCAACATTAACTTCTCAATTTGGCAAAGAGTTTGCTGGTCGTGCCGTTGAATTACGCAACAACAATAGCGTAAATGACTCGGTACGACTCCTTTTTTCGTAATATTTTAGTTTCGTTTGATAACAATTTCGTTTTTAATTATAACCCTTTCTAATTTATTAGGCTGATATTTTCCATTATTATTTGGTAGTTAATGCAAAAACTTTCTACACGGAAGCCAGATATGGAGAGTAAAATGAATCTTCTTAGAGTCATTGCTTCTGAAAATGGCATTACTTTGCAACTCAATGAACAGGTATATCATGAATCTTTAATATTAATCTTTGTGTCATTAGTTGTTTTTTTTTTTAATTTTATTTATGAAATTTCCCATTTTATTCTTTTTGTATATTACAGTTTTATTTTTAATCAAGTATTTCCTGTCACTGAATATAAAAGAAAGTTTGTTGTATTATCAAATTGAACAAAATAAAGGTTGACGTGTGGATTTAAAAACAAGATATAATAACAAATCATTGTGGGGCACGTTACTAATTTTGATTAGATCACATGAAATGTCTGTTTTTTTATAATTGGCTAAATATATGTTATGGGTCTTGTTTAAATATTCTTCGAAGAATTTTTAAAAATAGAAAAATAAAGAAAACTATTTATATAAATAGCAAAATTTTTAGATAGTTGTGATAGATGCTTATAGAAGTCTATCACTAATAGATGCTGATAGAATTTTATCAATGTCTTATCAATATTTTATTTGTTATTTTCTATAAATAGTTTGACATTTTTTCTATGGATGGGAAGTTTCATATTCTTTTTCTATTTAGTTTAATTTATAGATTTATTTGCAAATAGGAAAATGAACTAAAATATTTACAAATATTGCAAAATTTTATCATCTATATGTGATAGATCGCAATGGACTACTATCTGTGTCTAGAAAGTAAAATTTTACTATATTTGTAAATCTTTTCAGCAATTTTGTCATGTAAAATAATTTTCCTTAATTTATACTATTTGAAATAAATTTAATAGAGGAATTAAAATTTTTCTTCTCTTGAATGACCTTTTGCTCCAAATTTTAGTCAATTTATTAAATATTATGACATGCAAGTATTATATTACTCAATTTTACATTACTCGCAGGTGAGGGACTAGTTGAAAATTATTTTTAAAAAACAATGGGACTGAATTGAAGAAATATGACTAAAATATTGAGAAATTTTCATCGATAGAAAAAATGTCAAACTATTTACAGAAAATAGCAAAAAAAACACTGATAGACAATGATAGACTTCTATCAGCATCTATCAATGATAGACTGCTATCATTTCTATCACTGATAGATACTGATAGACTTCTATCAGCGTCTATCACAGCTATCTAAAAATTTTGCTATGTTGTGTAAATAGTTTTTCTCATTTTTCTATTTTTAAAAATTCTCCTAAAATATATATATTTAACTTTTTTTCATTTTATGCGTGGATATTTTAATTTTGTTGATGAAGTTTAAACATAAACACCATTTCGCTAATATAATTTAGGTTAAAATGTTATTTCAGAGTTTTTTGGGGTCATATGTGTATGTAAATAACTAGACACTAAGACTAAATGATATATTTAGCCCATTATTATTAATCTATTCCATTGATTAGGAATTTAGATATTAATTATTAGAATAATCATTAAAATTATTGATGGATAAGTAATTTATTTTTATTCTATTATTTAAATAATTCTTTTTTTTCCATTAAAACAGGGTGAGCCATCCAGTTCGATTCAAACTAGAAAACGAAACAGACAACAAAGCAGAAGTGCGGCTTCGACCACAAGCTCATATCAAACTCGCAGCTCAAGTCGCACAAGACGTTGATACATTTATGGGTTTTACTATATTTTTGTAATATTAATAGTTTATCCATTTTTAAATTTTATCGTTCTATAGATTTTTTAAAGATGTTCAATTCAATACTCGTGTGTTATTTGAATGTTTTAAGTTTTAACGTTTTAGAATTAAGGAAATAAATATCGA

mRNA sequence

GTGTGTTCAGATTAGATCAGAATTCAGATAATCTTGTTGTTGTTGAGCTTATAATTATAATTTTCTCCTTCTAATTTCTCTCTTGAGCTATCCGGAGAAAAAGAAAAGAAAAAAAAAAGAAAAGAATGAGAAGAATTGTGAAACGTGTTGTGGGAAGAAGTTTCAAGTCCTGTGGTTTCAGACGTCTGGTCCAATCTGCTTTCACGCGCCTCCAATCGCTGAAGAACCATCGCCATGGAAGATATTCCGAAGATGGAAACGACGCCGTTCGCCTCCTTCAAGATGGCCTTCCCAAACTTGCTCTCTCTCGCGAGGCAGTGTCAAGTGTAGTATTTGCAGCTTCAAGATGGAAGGATTTTCCCGAACTTGAAGACATTAAATCAACATTAACTTCTCAATTTGGCAAAGAGTTTGCTGGTCGTGCCGTTGAATTACGCAACAACAATAGCGTAAATGACTCGTTAATGCAAAAACTTTCTACACGGAAGCCAGATATGGAGAGTAAAATGAATCTTCTTAGAGTCATTGCTTCTGAAAATGGCATTACTTTGCAACTCAATGAACAGGGTGAGCCATCCAGTTCGATTCAAACTAGAAAACGAAACAGACAACAAAGCAGAAGTGCGGCTTCGACCACAAGCTCATATCAAACTCGCAGCTCAAGTCGCACAAGACGTTGATACATTTATGGGTTTTACTATATTTTTGTAATATTAATAGTTTATCCATTTTTAAATTTTATCGTTCTATAGATTTTTTAAAGATGTTCAATTCAATACTCGTGTGTTATTTGAATGTTTTAAGTTTTAACGTTTTAGAATTAAGGAAATAAATATCGA

Coding sequence (CDS)

ATGAGAAGAATTGTGAAACGTGTTGTGGGAAGAAGTTTCAAGTCCTGTGGTTTCAGACGTCTGGTCCAATCTGCTTTCACGCGCCTCCAATCGCTGAAGAACCATCGCCATGGAAGATATTCCGAAGATGGAAACGACGCCGTTCGCCTCCTTCAAGATGGCCTTCCCAAACTTGCTCTCTCTCGCGAGGCAGTGTCAAGTGTAGTATTTGCAGCTTCAAGATGGAAGGATTTTCCCGAACTTGAAGACATTAAATCAACATTAACTTCTCAATTTGGCAAAGAGTTTGCTGGTCGTGCCGTTGAATTACGCAACAACAATAGCGTAAATGACTCGTTAATGCAAAAACTTTCTACACGGAAGCCAGATATGGAGAGTAAAATGAATCTTCTTAGAGTCATTGCTTCTGAAAATGGCATTACTTTGCAACTCAATGAACAGGGTGAGCCATCCAGTTCGATTCAAACTAGAAAACGAAACAGACAACAAAGCAGAAGTGCGGCTTCGACCACAAGCTCATATCAAACTCGCAGCTCAAGTCGCACAAGACGTTGA

Protein sequence

MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLALSREAVSSVVFAASRWKDFPELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASENGITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSRTRR

Homology

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BLAST of Lsi08G003970 vs. ExPASy TrEMBL
Match: A0A1S3BD77 (uncharacterized protein LOC103488605 OS=Cucumis melo OX=3656 GN=LOC103488605 PE=3 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.2e-55
Identity = 135/226 (59.73%), Postives = 155/226 (68.58%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           MRRI+ RVVGRSF+S GF+RLVQS+FTRLQSL +HR  RYSED ND + LLQDGL KLAL
Sbjct: 1   MRRILNRVVGRSFRSSGFKRLVQSSFTRLQSLTDHRRRRYSEDLNDVLSLLQDGLHKLAL 60

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EA SSVVFAASRWKDF
Sbjct: 61  SRCEQMIKHQNLLDAYGLIEGYLNLLMERIQLLGRARECPDELKEAASSVVFAASRWKDF 120

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
            EL DIKS  TSQFGKEF   AVELRNNN VN S++QKLST+KPDM+SKMNLL++IAS+ 
Sbjct: 121 TELGDIKSIFTSQFGKEFTAPAVELRNNNRVNQSIIQKLSTKKPDMKSKMNLLKLIASDK 180

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSRTRR 185
           GI +QLN+QGEP  SIQTRKR+R+Q+    +TTSSYQTR+SSR  R
Sbjct: 181 GIVVQLNDQGEPFGSIQTRKRSREQNEVVIATTSSYQTRNSSRKLR 226

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BLAST of Lsi08G003970 vs. ExPASy TrEMBL
Match: A0A0A0K949 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G031970 PE=3 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.4e-48
Identity = 120/223 (53.81%), Postives = 150/223 (67.26%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           MR+I+KRV+GRSF S  F+R VQS+FTRL+SL +HR  R+SED N+ V LLQ+ L +LAL
Sbjct: 1   MRKIMKRVLGRSFSSSCFKRSVQSSFTRLKSLTDHRRRRFSEDLNEVVSLLQEDLQELAL 60

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EAVSSV+FAASRWKDF
Sbjct: 61  SRCEQMIKHQNLVDAYGLIEGYLNLLIERIHLLGRERECPDELKEAVSSVIFAASRWKDF 120

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
            EL D+KS  TSQFGKEF  RAVELRNNN VN S++QKLS +KPD +SKMNLL++IAS+ 
Sbjct: 121 TELGDVKSIFTSQFGKEFTARAVELRNNNRVNQSIIQKLSAKKPDTKSKMNLLKLIASDK 180

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSR 182
           GI ++LNE+G+ S SIQ RKR R++++   +TTSSYQTR+SSR
Sbjct: 181 GIVVKLNEEGKLSKSIQIRKRGREKNKVVTATTSSYQTRNSSR 223

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BLAST of Lsi08G003970 vs. ExPASy TrEMBL
Match: A0A6J1HHW4 (uncharacterized protein LOC111463790 OS=Cucurbita moschata OX=3662 GN=LOC111463790 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 7.8e-47
Identity = 124/223 (55.61%), Postives = 147/223 (65.92%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           +RR+ K ++GRS +S  FRRLVQ+A   L++LK  RHGRY ED  DA RLL +GLPKLAL
Sbjct: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLAL 63

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EAVSSVVFAASR +DF
Sbjct: 64  SRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDF 123

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
           PELE+IKS L+S+FG EFA RAVELRNNN+VN S+M KLSTRKP++ESKMNLLRVIASEN
Sbjct: 124 PELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSIMLKLSTRKPNLESKMNLLRVIASEN 183

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSR 182
           G TLQLN++GEP S   T    RQ+ R A+ST  +Y+TRSSSR
Sbjct: 184 GFTLQLNDKGEPISCTAT----RQRKRGASSTKRTYRTRSSSR 221

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BLAST of Lsi08G003970 vs. ExPASy TrEMBL
Match: A0A6J1I6P0 (uncharacterized protein LOC111470458 OS=Cucurbita maxima OX=3661 GN=LOC111470458 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 1.2e-44
Identity = 120/224 (53.57%), Postives = 144/224 (64.29%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           +RR+ K ++GRS +S  FRRLVQ+A   L++LK   HGRY ED  D  RLL +GLPKLAL
Sbjct: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLHHGRYVEDRKDTARLLHEGLPKLAL 63

Query: 61  SR-------------------------------------------EAVSSVVFAASRWKD 120
           SR                                           EAVSSVVFAASR +D
Sbjct: 64  SRCEQMIRHQNLMDAYGMMEGYLNLLRERMHLLLAPGRECPKELEEAVSSVVFAASRCED 123

Query: 121 FPELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASE 180
           FPEL++IKS L+S+FG EFA RAVELRNNN+VN S+M KLSTRKP++ESKM+LLRVIASE
Sbjct: 124 FPELQEIKSVLSSRFGTEFAARAVELRNNNTVNHSIMLKLSTRKPNLESKMHLLRVIASE 183

Query: 181 NGITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSR 182
           NG TLQLN++GEP S   T    RQ+ R A ST  +Y+TRSSSR
Sbjct: 184 NGFTLQLNDKGEPISCTAT----RQRKRGATSTKRTYRTRSSSR 222

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BLAST of Lsi08G003970 vs. ExPASy TrEMBL
Match: A0A6J1CEI4 (uncharacterized protein LOC111010490 OS=Momordica charantia OX=3673 GN=LOC111010490 PE=3 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 6.4e-41
Identity = 123/245 (50.20%), Postives = 152/245 (62.04%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLL-QDGLPKLA 60
           MRRI+  ++GR+FKS  FRRL+ SAFTRLQSL  HR  RYS+D +D VRLL QDGL  LA
Sbjct: 1   MRRILNPLLGRTFKSSNFRRLIHSAFTRLQSLNTHRRRRYSQDRDDVVRLLQQDGLHNLA 60

Query: 61  LSR------------------------------------------EAVSSVVFAASRWKD 120
           LSR                                          EA S VV+AASR +D
Sbjct: 61  LSRCEQVIKHQNLLDAYGMIEGYLNLLMDRIYLLVQQRECPDELKEAASGVVYAASRCRD 120

Query: 121 FPELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASE 180
           FPELE+IKS LTS+FG+EF+GRAVELRNN+ VN+ +MQKLSTRKP+MESKMNLLR IASE
Sbjct: 121 FPELEEIKSVLTSRFGREFSGRAVELRNNSGVNELMMQKLSTRKPNMESKMNLLRAIASE 180

Query: 181 NGITLQLNEQGEPSSSIQTR-KRNRQQSR----------SAAST--------TSSYQTRS 184
           N I LQL+ Q +PS   Q + + N++++R          S++ST        TS Y+TRS
Sbjct: 181 NVIALQLH-QDQPSLPYQEKLEGNKRENRGGPIAKKSNGSSSSTSMHDKPKNTSPYRTRS 240

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BLAST of Lsi08G003970 vs. NCBI nr
Match: XP_038884436.1 (uncharacterized protein LOC120075284 isoform X1 [Benincasa hispida])

HSP 1 Score: 240.7 bits (613), Expect = 9.7e-60
Identity = 145/226 (64.16%), Postives = 160/226 (70.80%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           MRRI+  +VGRSFKS  FRRLVQSAFTRL++LKNH H RYSE  +D VRLLQDGL  LAL
Sbjct: 1   MRRILNPLVGRSFKSSDFRRLVQSAFTRLRALKNHHHRRYSEGRDDVVRLLQDGLSGLAL 60

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EAVSSVVFA+SRWKDF
Sbjct: 61  SRCEEMIEHQNLLDAYGMIEGYLNLLMESIYLLGRGRECPDELKEAVSSVVFASSRWKDF 120

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
            ELEDIKS LTSQFGKEF GRAVELRNNNSVNDS+MQKLSTRKP+M+SKMNLL+VIAS+N
Sbjct: 121 SELEDIKSILTSQFGKEFTGRAVELRNNNSVNDSIMQKLSTRKPNMDSKMNLLKVIASDN 180

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSRTRR 185
           GITLQLNE+    SSIQTRKR+RQQ+RS    T+SYQTRSSSR  R
Sbjct: 181 GITLQLNEEDALCSSIQTRKRSRQQNRSETLATNSYQTRSSSRKHR 226

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BLAST of Lsi08G003970 vs. NCBI nr
Match: XP_008445649.1 (PREDICTED: uncharacterized protein LOC103488605 [Cucumis melo])

HSP 1 Score: 226.1 bits (575), Expect = 2.5e-55
Identity = 135/226 (59.73%), Postives = 155/226 (68.58%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           MRRI+ RVVGRSF+S GF+RLVQS+FTRLQSL +HR  RYSED ND + LLQDGL KLAL
Sbjct: 1   MRRILNRVVGRSFRSSGFKRLVQSSFTRLQSLTDHRRRRYSEDLNDVLSLLQDGLHKLAL 60

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EA SSVVFAASRWKDF
Sbjct: 61  SRCEQMIKHQNLLDAYGLIEGYLNLLMERIQLLGRARECPDELKEAASSVVFAASRWKDF 120

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
            EL DIKS  TSQFGKEF   AVELRNNN VN S++QKLST+KPDM+SKMNLL++IAS+ 
Sbjct: 121 TELGDIKSIFTSQFGKEFTAPAVELRNNNRVNQSIIQKLSTKKPDMKSKMNLLKLIASDK 180

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSRTRR 185
           GI +QLN+QGEP  SIQTRKR+R+Q+    +TTSSYQTR+SSR  R
Sbjct: 181 GIVVQLNDQGEPFGSIQTRKRSREQNEVVIATTSSYQTRNSSRKLR 226

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BLAST of Lsi08G003970 vs. NCBI nr
Match: XP_011656518.1 (uncharacterized protein LOC101210797 [Cucumis sativus] >KGN45948.1 hypothetical protein Csa_005219 [Cucumis sativus])

HSP 1 Score: 202.6 bits (514), Expect = 2.9e-48
Identity = 120/223 (53.81%), Postives = 150/223 (67.26%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           MR+I+KRV+GRSF S  F+R VQS+FTRL+SL +HR  R+SED N+ V LLQ+ L +LAL
Sbjct: 1   MRKIMKRVLGRSFSSSCFKRSVQSSFTRLKSLTDHRRRRFSEDLNEVVSLLQEDLQELAL 60

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EAVSSV+FAASRWKDF
Sbjct: 61  SRCEQMIKHQNLVDAYGLIEGYLNLLIERIHLLGRERECPDELKEAVSSVIFAASRWKDF 120

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
            EL D+KS  TSQFGKEF  RAVELRNNN VN S++QKLS +KPD +SKMNLL++IAS+ 
Sbjct: 121 TELGDVKSIFTSQFGKEFTARAVELRNNNRVNQSIIQKLSAKKPDTKSKMNLLKLIASDK 180

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSR 182
           GI ++LNE+G+ S SIQ RKR R++++   +TTSSYQTR+SSR
Sbjct: 181 GIVVKLNEEGKLSKSIQIRKRGREKNKVVTATTSSYQTRNSSR 223

[Close]

BLAST of Lsi08G003970 vs. NCBI nr
Match: XP_022963468.1 (uncharacterized protein LOC111463790 [Cucurbita moschata])

HSP 1 Score: 196.8 bits (499), Expect = 1.6e-46
Identity = 124/223 (55.61%), Postives = 147/223 (65.92%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           +RR+ K ++GRS +S  FRRLVQ+A   L++LK  RHGRY ED  DA RLL +GLPKLAL
Sbjct: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLAL 63

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EAVSSVVFAASR +DF
Sbjct: 64  SRCEQMFRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDF 123

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
           PELE+IKS L+S+FG EFA RAVELRNNN+VN S+M KLSTRKP++ESKMNLLRVIASEN
Sbjct: 124 PELEEIKSVLSSRFGTEFAARAVELRNNNTVNHSIMLKLSTRKPNLESKMNLLRVIASEN 183

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSR 182
           G TLQLN++GEP S   T    RQ+ R A+ST  +Y+TRSSSR
Sbjct: 184 GFTLQLNDKGEPISCTAT----RQRKRGASSTKRTYRTRSSSR 221

[Close]

BLAST of Lsi08G003970 vs. NCBI nr
Match: XP_023538969.1 (uncharacterized protein LOC111799739 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 193.0 bits (489), Expect = 2.3e-45
Identity = 123/223 (55.16%), Postives = 145/223 (65.02%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           +RR+ K ++GRS +S  FRRLVQ+A   L++LK  RHGRY ED  DA RLL +GLPKLAL
Sbjct: 4   LRRVFKPLLGRS-RSSSFRRLVQNALEHLETLKKLRHGRYVEDRKDAARLLHEGLPKLAL 63

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
           SR                                          EAVSSVVFAASR +DF
Sbjct: 64  SRCEQMIRHQNLMDAYGMMEGYLNLLRERLYLLAPGRECPKELEEAVSSVVFAASRCEDF 123

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 180
           PELE+IKS L+S+FG+EFA RAVELR NN+VN S+M KLSTRKP++ESKMNLLRVIASEN
Sbjct: 124 PELEEIKSVLSSRFGREFAARAVELRINNTVNHSIMLKLSTRKPNLESKMNLLRVIASEN 183

Query: 181 GITLQLNEQGEPSSSIQTRKRNRQQSRSAASTTSSYQTRSSSR 182
           G TLQLN++GEP S   T    RQ+ R A ST   Y+TRSSSR
Sbjct: 184 GFTLQLNDKGEPISCTAT----RQRKRGATSTKRIYRTRSSSR 221

[Close]

BLAST of Lsi08G003970 vs. TAIR 10
Match: AT1G13340.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 97.1 bits (240), Expect = 1.6e-20
Identity = 73/213 (34.27%), Postives = 106/213 (49.77%), Query Frame = 0


Query: 1   MRRIVKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLAL 60
           M + +  ++GRSFK+  F+ L+  A TRL  LKN R  R S+  +D   LL+ G  + A 
Sbjct: 1   MGKKLDALLGRSFKTNKFKSLITLALTRLSILKNQRQARLSQAISDVTELLKLGQHEHAY 60

Query: 61  SR------------------------------------------EAVSSVVFAASRWKDF 120
            R                                          EAVS ++FAASR  +F
Sbjct: 61  HRVDQVVKDQNTLDVLFFIHGYFTLCLDRIHLFEHNRDCPEELLEAVSGLLFAASRIGEF 120

Query: 121 PELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASEN 172
           PEL++I++ L S+FGK+ A R++ELR+N  V+  ++QKLSTR P  E +M  L+ IA+EN
Sbjct: 121 PELQEIRNVLISRFGKDLAARSIELRSNCGVDPKIIQKLSTRPPPKEVRMKALKEIAAEN 180

[Close]

BLAST of Lsi08G003970 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.1e-11
Identity = 34/77 (44.16%), Postives = 50/77 (64.94%), Query Frame = 0


Query: 62  REAVSSVVFAASRWKDFPELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRK 121
           +EA+SSV FAA R  D  EL+ ++    S++GKEF   A EL+ ++ VN  L++ LS R 
Sbjct: 103 KEAISSVCFAAPRCSDLTELQQVQILFVSKYGKEFVAAASELKPDSGVNRKLVELLSVRA 162

Query: 122 PDMESKMNLLRVIASEN 139
           P  E+K+ LL+ IA E+
Sbjct: 163 PSPETKLKLLKEIAEEH 179

[Close]

BLAST of Lsi08G003970 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 65.5 bits (158), Expect = 5.2e-11
Identity = 57/213 (26.76%), Postives = 96/213 (45.07%), Query Frame = 0


Query: 5   VKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLALSR-- 64
           +K+V+ R FK    +  +Q A +RL+ LKN +  +  +   +  +LL+ G    A  R  
Sbjct: 1   MKKVLQRGFKPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVE 60

Query: 65  ----------------------------------------EAVSSVVFAASRWKDFPELE 124
                                                   EAV+SV+FA+ R  D PEL 
Sbjct: 61  HVVREEKTVAAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELS 120

Query: 125 DIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASENGITL 174
           +I    T+++GK+F+  AVELR ++ V+  L++KLS + PD  +K+ +L  IA E+ +  
Sbjct: 121 EIFKQFTTKYGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEEHNVVW 180

[Close]

BLAST of Lsi08G003970 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 62.0 bits (149), Expect = 5.8e-10
Identity = 29/76 (38.16%), Postives = 47/76 (61.84%), Query Frame = 0


Query: 62  REAVSSVVFAASRWKDFPELEDIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRK 121
           +E ++S++FAA R  + PEL D++     ++GK+F   A +LR +  VN  L+ KLS R 
Sbjct: 117 KEGIASLIFAAPRCSEIPELGDLRDIFAKKYGKDFVSAATDLRPSCGVNRMLIDKLSVRN 176

Query: 122 PDMESKMNLLRVIASE 138
           P  E K+ +++ IA E
Sbjct: 177 PGGEYKLKIMKEIAKE 192

[Close]

BLAST of Lsi08G003970 vs. TAIR 10
Match: AT4G29440.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 57.0 bits (136), Expect = 1.9e-08
Identity = 55/222 (24.77%), Postives = 100/222 (45.05%), Query Frame = 0


Query: 5   VKRVVGRSFKSCGFRRLVQSAFTRLQSLKNHRHGRYSEDGNDAVRLLQDGLPKLALSR-- 64
           +K+V+ RSFK    +  +Q A +RL+ LKN +  +  +   +   LL+ G  + A  R  
Sbjct: 1   MKKVLHRSFKPAKCKIALQMAASRLKILKNKKDTQIKQLRRELAHLLESGQTQTAKIRVE 60

Query: 65  ----------------------------------------EAVSSVVFAASRWKDFPELE 124
                                                   EAV+SV++A+ R  D  EL 
Sbjct: 61  HVVREEKTVAAYELVGIYCELLVARLGVIDSQKTCPNDLKEAVASVLYASQRLTDVGELS 120

Query: 125 DIKSTLTSQFGKEFAGRAVELRNNNSVNDSLMQKLSTRKPDMESKMNLLRVIASENGITL 184
           DI    ++++GK+F   A+ L+ ++ V+  L++KLS + PD  +K+ +L  IA+++ +T 
Sbjct: 121 DIVKHFSAKYGKDFVSAAIGLQPDSGVSRLLVEKLSVKAPDGPTKIKILTEIATQHNVT- 180

The following BLAST results are available for this feature:

BLAST of Lsi08G003970 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi08G003970 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BD77	1.2e-55	59.73	uncharacterized protein LOC103488605 OS=Cucumis melo OX=3656 GN=LOC103488605 PE=...	[more]
A0A0A0K949	1.4e-48	53.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G031970 PE=3 SV=1	[more]
A0A6J1HHW4	7.8e-47	55.61	uncharacterized protein LOC111463790 OS=Cucurbita moschata OX=3662 GN=LOC1114637...	[more]
A0A6J1I6P0	1.2e-44	53.57	uncharacterized protein LOC111470458 OS=Cucurbita maxima OX=3661 GN=LOC111470458...	[more]
A0A6J1CEI4	6.4e-41	50.20	uncharacterized protein LOC111010490 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]

BLAST of Lsi08G003970 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884436.1	9.7e-60	64.16	uncharacterized protein LOC120075284 isoform X1 [Benincasa hispida]	[more]
XP_008445649.1	2.5e-55	59.73	PREDICTED: uncharacterized protein LOC103488605 [Cucumis melo]	[more]
XP_011656518.1	2.9e-48	53.81	uncharacterized protein LOC101210797 [Cucumis sativus] >KGN45948.1 hypothetical ...	[more]
XP_022963468.1	1.6e-46	55.61	uncharacterized protein LOC111463790 [Cucurbita moschata]	[more]
XP_023538969.1	2.3e-45	55.16	uncharacterized protein LOC111799739 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi08G003970 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G13340.1	1.6e-20	34.27	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT1G34220.2	3.1e-11	44.16	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT2G19710.1	5.2e-11	26.76	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT4G35730.1	5.8e-10	38.16	Regulator of Vps4 activity in the MVB pathway protein	[more]
AT4G29440.1	1.9e-08	24.77	Regulator of Vps4 activity in the MVB pathway protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR042277	Vacuolar protein sorting-associated protein IST1-like	GENE3D	1.20.1260.60		coord: 60..150 e-value: 1.4E-23 score: 85.3
IPR005061	Vacuolar protein sorting-associated protein Ist1	PFAM	PF03398	Ist1	coord: 62..140 e-value: 1.4E-20 score: 73.8
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 62..173
IPR005061	Vacuolar protein sorting-associated protein Ist1	PANTHER	PTHR12161	IST1 FAMILY MEMBER	coord: 8..62
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 142..184
None	No IPR available	PANTHER	PTHR12161:SF16	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 8..62
None	No IPR available	PANTHER	PTHR12161:SF16	REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEIN	coord: 62..173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G003970.1	Lsi08G003970.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport
molecular_function	GO:0016301	kinase activity