Lsi08G003900 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G003900
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	adenylate isopentenyltransferase-like
Location	chr08: 10832506 .. 10833468 (+)
RNA-Seq Expression	Lsi08G003900
Synteny	Lsi08G003900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACTTTCCCTCCCCTTCTCCCACTCTTTTCCTCTTTCTCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGTTTCCTCCGATTCCCTCCTCCTCCTCCTTGGCGCTACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGGGGCCTCGACATCACCACTAACAAGCTCCCTCTCTCCCACCGCCGCGCCGTCCCCCATCATCTTCTTGGCGATTTCGACCCTCTCGCCGGTGAGGTTTCTCCTGCCGCCTTCCGTTCTCATGCCTCCGCCGTCATCTCCGGCGTCGTCTCCCGAGGCAACCTTCCTCTTGTCGTTGGCGGCTCCAACTCCTTCATTCACGCTCTCGTTGCCGCTGATTTCGATGCTACCCAGGACGTTTTCGGCGGGCCTCGGAATCGGGTGTCGGCGGAGTTTCGGTATCGGTGCTGCTTTTTGTGGGTCGACGTGTCGTTTCCGGTTCTGAAACGGTATTTGTCGAATCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGGGGAGTTGGCGGAGTTTTATGACCCGGAAATGGCTGATTCGGAGCCCCGAACCGGGCTGAGAAAAGCCATTGGAGTGCCCGAATTCGAGGAGTTTTTCCGGCGGCATCATCCGAAAGGGAGGGATTACAGAGAGGGGGATCCACTGCGGGCGAGCGCATACGAAGAAGCGGTAAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGTGGAAGATCGGACGGCTCAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTCCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGAGCTTCATTCAGGAGTAG

mRNA sequence

ATGACACTTTCCCTCCCCTTCTCCCACTCTTTTCCTCTTTCTCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGTTTCCTCCGATTCCCTCCTCCTCCTCCTTGGCGCTACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGGGGCCTCGACATCACCACTAACAAGCTCCCTCTCTCCCACCGCCGCGCCGTCCCCCATCATCTTCTTGGCGATTTCGACCCTCTCGCCGGTGAGGTTTCTCCTGCCGCCTTCCGTTCTCATGCCTCCGCCGTCATCTCCGGCGTCGTCTCCCGAGGCAACCTTCCTCTTGTCGTTGGCGGCTCCAACTCCTTCATTCACGCTCTCGTTGCCGCTGATTTCGATGCTACCCAGGACGTTTTCGGCGGGCCTCGGAATCGGGTGTCGGCGGAGTTTCGGTATCGGTGCTGCTTTTTGTGGGTCGACGTGTCGTTTCCGGTTCTGAAACGGTATTTGTCGAATCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGGGGAGTTGGCGGAGTTTTATGACCCGGAAATGGCTGATTCGGAGCCCCGAACCGGGCTGAGAAAAGCCATTGGAGTGCCCGAATTCGAGGAGTTTTTCCGGCGGCATCATCCGAAAGGGAGGGATTACAGAGAGGGGGATCCACTGCGGGCGAGCGCATACGAAGAAGCGGTAAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGTGGAAGATCGGACGGCTCAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTCCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGAGCTTCATTCAGGAGTAG

Coding sequence (CDS)

ATGACACTTTCCCTCCCCTTCTCCCACTCTTTTCCTCTTTCTCGCCGCCCCCGATGCCCTCTTATCCTCTCCTCTGTTTCCTCCGATTCCCTCCTCCTCCTCCTTGGCGCTACCGGCTCCGGCAAGTCCTCCCTCTCCATCGACCTCGCCGCCCGCTTCCCTTCCGAGATCATCAATTCCGACAAAATGCAGCTCTACAGGGGCCTCGACATCACCACTAACAAGCTCCCTCTCTCCCACCGCCGCGCCGTCCCCCATCATCTTCTTGGCGATTTCGACCCTCTCGCCGGTGAGGTTTCTCCTGCCGCCTTCCGTTCTCATGCCTCCGCCGTCATCTCCGGCGTCGTCTCCCGAGGCAACCTTCCTCTTGTCGTTGGCGGCTCCAACTCCTTCATTCACGCTCTCGTTGCCGCTGATTTCGATGCTACCCAGGACGTTTTCGGCGGGCCTCGGAATCGGGTGTCGGCGGAGTTTCGGTATCGGTGCTGCTTTTTGTGGGTCGACGTGTCGTTTCCGGTTCTGAAACGGTATTTGTCGAATCGGGTGGACGACATGTTGGAGGCTGGGATGGTGGGGGAGTTGGCGGAGTTTTATGACCCGGAAATGGCTGATTCGGAGCCCCGAACCGGGCTGAGAAAAGCCATTGGAGTGCCCGAATTCGAGGAGTTTTTCCGGCGGCATCATCCGAAAGGGAGGGATTACAGAGAGGGGGATCCACTGCGGGCGAGCGCATACGAAGAAGCGGTAAGGAAAATAAAGGAGAACACGTGGCAGCTGACGAAGAGGCAGTTGTGGAAGATCGGACGGCTCAGAAGGGCTGGGTGGGACCTGAAGAGAGTGGACGCCACGGCGGCGGTTGTGGCGGTCCTGGCGTCGGAAGCGGCGGAGAAACGGTCAGAAATATGGGAGACACAGGTGGTGGAACCAAGCGTAAAGATTGTGAAGAGCTTCATTCAGGAGTAG

Protein sequence

MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQE

Homology

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BLAST of Lsi08G003900 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 8.9e-88
Identity = 177/301 (58.80%), Postives = 216/301 (71.76%), Query Frame = 0


Query: 29  DSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHL 88
           + LL+L+GATG+GKS LSIDLAA FP E+INSDKMQ+Y+GLDITTNK+ +  R  VPHHL
Sbjct: 30  EKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHL 89

Query: 89  LGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDAT-QDVF 148
           LG+ DP  GE++PA FRS A   +S +  R  LP++VGGSNSFIHAL+   FD++   VF
Sbjct: 90  LGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVF 149

Query: 149 -GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPE----M 208
             G  + VS+E RY CCFLWVDVS  VL  YL+ RVDDMLE GM  ELAEFY PE     
Sbjct: 150 EEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHD 209

Query: 209 ADSEPRTGLRKAIGVPEFEEFFRRHHP---KGRDYREGDPLRASAYEEAVRKIKENTWQL 268
            DS  RTGLRKAIGVPEF+ +F +  P   +G D    D +R  A+EEAVR IKENT  L
Sbjct: 210 EDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGR-DRVRRGAFEEAVRAIKENTCHL 269

Query: 269 TKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQ 321
            KRQ+ KI RL+ AGWDL+R+DAT +  A + S++ EK +EIWE QV+EPSVKIV  F+ 
Sbjct: 270 AKRQIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFLD 329

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BLAST of Lsi08G003900 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 306.2 bits (783), Expect = 4.3e-82
Identity = 162/315 (51.43%), Postives = 216/315 (68.57%), Query Frame = 0


Query: 10  SFPLSRRPRCPLI--------LSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+         S    D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNR 189
           P++ GGSNSF+HAL+A  FD   D F      +S++ RY CCF+WVDVS  VL  YL  R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLR 249
           VD+M+++GM  EL+ FYDP  +  E R G+RKAIGVPEF+ +F+ + P+ +   + D LR
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALR 277

Query: 250 ASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKR-SE 309
            +AY++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+   ++EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

[Close]

BLAST of Lsi08G003900 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 1.7e-75
Identity = 155/299 (51.84%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 29  DSLLLLLGATGSGKSSLSIDLAAR-FPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 88
           D ++L+ G TG+GKS LS+DLA R FP+EIINSDKMQ+Y+G +I TN +PL  +  VPHH
Sbjct: 45  DKVVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHH 104

Query: 89  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 148
           LLG F P  GE++PA FRS A+  IS ++S   LP+VVGGSNSF HAL+A  FD   D F
Sbjct: 105 LLGQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPF 164

Query: 149 --GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADS 208
             G   + + ++ RY+CC LWVDV  PVL ++L NRVD M+E+G+V +LAE YDP + DS
Sbjct: 165 SPGSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDS 224

Query: 209 EPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLW 268
             R G+RK IGV EF+ +F R +PK  D    D  R +AYEE V+ +KE T +L K+Q  
Sbjct: 225 GRRLGVRKTIGVEEFDRYF-RVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKE 284

Query: 269 KIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRS----EIWETQVVEPSVKIVKSFIQE 321
           KI +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 285 KIMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]

BLAST of Lsi08G003900 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-74
Identity = 149/298 (50.00%), Postives = 204/298 (68.46%), Query Frame = 0


Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS+IHAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEP 207
              +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  EP
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKI 267
             G+ KAIGV EF+++ +  +    D  + DP+R  AYE+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-AAEKRSEIWETQVVEPSVKIVKSFIQE 321
            +LR AGWD+K+VDATA    A+ A    E  AE + +IW  +V+EP VKIV+S + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]

BLAST of Lsi08G003900 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.8e-72
Identity = 144/309 (46.60%), Postives = 198/309 (64.08%), Query Frame = 0


Query: 12  PLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDI 71
           P S  P   + +       +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +
Sbjct: 26  PRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKV 85

Query: 72  TTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSF 131
           TTN++ +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF
Sbjct: 86  TTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSF 145

Query: 132 IHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMV 191
           IHAL+   FD     F      +S+  RY CCFLWVDVS  VL  YLS RVD M+E+GM 
Sbjct: 146 IHALLVDRFDPKTYPFSS-ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMF 205

Query: 192 GELAEFYDPEMADSEPRT-GLRKAIGVPEFEEFFRRHHPKGRD-YREGDPLRASAYEEAV 251
            ELA FYDP  + S  R  G+ K IG+PEF+ +F  + P+ +    E D  R  AY+EAV
Sbjct: 206 EELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAV 265

Query: 252 RKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEP 311
           ++IKENTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ 
Sbjct: 266 QEIKENTWRLAKKQIERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDE 325

Query: 312 SVKIVKSFI 319
           S+K+VK F+
Sbjct: 326 SIKVVKRFL 325

[Close]

BLAST of Lsi08G003900 vs. ExPASy TrEMBL
Match: A0A5D3CF13 (Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold383G00180 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.5e-166
Identity = 298/320 (93.12%), Postives = 308/320 (96.25%), Query Frame = 0


Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFPLSRRPRCPL+LSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSFIHALVAADFD TQDVF G RNRVSAEFRYRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GM+ ELA+FYDPEMAD E R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +ASA+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Lsi08G003900 vs. ExPASy TrEMBL
Match: A0A1S3BD73 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.5e-166
Identity = 298/320 (93.12%), Postives = 308/320 (96.25%), Query Frame = 0


Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFPLSRRPRCPL+LSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSFIHALVAADFD TQDVF G RNRVSAEFRYRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GM+ ELA+FYDPEMAD E R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +ASA+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Lsi08G003900 vs. ExPASy TrEMBL
Match: A0A0A0K943 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.1e-164
Identity = 297/320 (92.81%), Postives = 305/320 (95.31%), Query Frame = 0


Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPLILSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSF+HALVAADFDATQDVF G RNRVS EF YRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GMV ELA+FYDPEMADSE R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +A A+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Lsi08G003900 vs. ExPASy TrEMBL
Match: A0A6J1HF40 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463593 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-150
Identity = 283/334 (84.73%), Postives = 297/334 (88.92%), Query Frame = 0


Query: 1   MTLSLPFSHS----------FPL----SRRPRCPLILSSVSSDSLLLLLGATGSGKSSLS 60
           MTLSLPFSHS          FPL    SRR + P +LSS+ SD+LLLLLGATGSGKSSLS
Sbjct: 1   MTLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRMLSSLPSDNLLLLLGATGSGKSSLS 60

Query: 61  IDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRS 120
           ID+AARFPSEIINSDKMQLYRGLD+TTNKLP S RR VPHHLLGDFDPLAGEVS AAFRS
Sbjct: 61  IDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFRS 120

Query: 121 HASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLW 180
            ASAVISGVVSRGNLP+VVGGSNS+IHALV A FD   DVF GP NRVSAEFRYRCCFLW
Sbjct: 121 QASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYRCCFLW 180

Query: 181 VDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRR 240
           VDVSFPVLK+YLS RVDDMLEAGMVGELAEFYDPEMA++EPR GLRKAIGVPEFEEFFRR
Sbjct: 181 VDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFRR 240

Query: 241 HHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAV 300
           +HPKGR+YREGDPLRA AYEEAVRKIKENTWQLTKRQL KIGRLR AGWDLKRVDATAAV
Sbjct: 241 YHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATAAV 300

Query: 301 VAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQE 321
           VAVLASEAAEKRSEIWE QVVEPSVKIVK FI+E
Sbjct: 301 VAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

[Close]

BLAST of Lsi08G003900 vs. ExPASy TrEMBL
Match: A0A6J1I7X6 (adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111470452 PE=3 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.5e-149
Identity = 281/334 (84.13%), Postives = 296/334 (88.62%), Query Frame = 0


Query: 1   MTLSLPFSHS----------FPL----SRRPRCPLILSSVSSDSLLLLLGATGSGKSSLS 60
           MTLSLPFSHS          FPL    SRR + P +LSS+ SD+LLLLLGATGSGKSSLS
Sbjct: 1   MTLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRMLSSLPSDNLLLLLGATGSGKSSLS 60

Query: 61  IDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRS 120
           ID+AARFPSEIINSDKMQLYRGLD+TTNKLP S RR VPHHLLGDFDPLAGEVS AAFRS
Sbjct: 61  IDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFRS 120

Query: 121 HASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLW 180
            ASAVISGVVSRGNLP+VVGGSNS+IHALV A  D   DVF GP NRVSAEFRYRCCFLW
Sbjct: 121 QASAVISGVVSRGNLPVVVGGSNSYIHALVTAGLDQDYDVFDGPLNRVSAEFRYRCCFLW 180

Query: 181 VDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRR 240
           VDVSFPVLK+YLS RVDDMLEAGMVGELAEFYDPEMA++EPR GLRKAIGVPEFEEFFRR
Sbjct: 181 VDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFRR 240

Query: 241 HHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAV 300
           +HPKGR+YREGDPLR+ AYEEAVRKIKENTWQLTKRQL KIGRLR AGWDLKRVDATAAV
Sbjct: 241 YHPKGREYREGDPLRSGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATAAV 300

Query: 301 VAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQE 321
           VAVLASEAAEKRSEIWE QVVEPSVKIVK FI+E
Sbjct: 301 VAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

[Close]

BLAST of Lsi08G003900 vs. NCBI nr
Match: XP_008445640.1 (PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa] >TYK08909.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 593.2 bits (1528), Expect = 1.3e-165
Identity = 298/320 (93.12%), Postives = 308/320 (96.25%), Query Frame = 0


Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFPLSRRPRCPL+LSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRPRCPLVLSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGD+DPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSFIHALVAADFD TQDVF G RNRVSAEFRYRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GM+ ELA+FYDPEMAD E R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +ASA+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE
Sbjct: 241 QASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Lsi08G003900 vs. NCBI nr
Match: XP_004150914.1 (adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical protein Csa_005709 [Cucumis sativus])

HSP 1 Score: 588.2 bits (1515), Expect = 4.3e-164
Identity = 297/320 (92.81%), Postives = 305/320 (95.31%), Query Frame = 0


Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSF LSRRPRCPLILSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFLLSRRPRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGV+SRGN
Sbjct: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVISRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSF+HALVAADFDATQDVF G RNRVS EF YRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+ML+ GMV ELA+FYDPEMADSE R GLRKAIGVPEFEE+FRRHHPKGRDYREGDPL
Sbjct: 181 RVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSE 300
           +A A+EEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASE AEKRSE
Sbjct: 241 QARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEVAEKRSE 300

Query: 301 IWETQVVEPSVKIVKSFIQE 321
           IWETQVVEPSVKIVK FIQE
Sbjct: 301 IWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Lsi08G003900 vs. NCBI nr
Match: XP_038886381.1 (adenylate isopentenyltransferase 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 555.4 bits (1430), Expect = 3.1e-154
Identity = 280/307 (91.21%), Postives = 291/307 (94.79%), Query Frame = 0


Query: 1   MTLSLPFSHSFPLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60
           MTLSLPFSHSFPLSRR RCPLILSS  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINS
Sbjct: 1   MTLSLPFSHSFPLSRRRRCPLILSSAPSDSLLLLLGATGSGKSSLSIDLAARFPSEIINS 60

Query: 61  DKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGN 120
           DKMQLY GLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHA++VISGVVSRGN
Sbjct: 61  DKMQLYGGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHAASVISGVVSRGN 120

Query: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSN 180
           LPLVVGGSNSFIHALVAADFDATQDVFGG RNR+SAEFRYRCCFLWVDVSFPVLKRYLS 
Sbjct: 121 LPLVVGGSNSFIHALVAADFDATQDVFGGSRNRISAEFRYRCCFLWVDVSFPVLKRYLSK 180

Query: 181 RVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPL 240
           RVD+MLE+GMV ELAEFYDPEMA+SEPR GLRKAIGVPEFEEFFRR+HPKGRDYREGDPL
Sbjct: 181 RVDEMLESGMVEELAEFYDPEMAESEPRIGLRKAIGVPEFEEFFRRYHPKGRDYREGDPL 240

Query: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEK--- 300
           RASAYEEAVRKIKENTWQLTKRQLWKIGRLR+AGWDLKRVDATAAVVAVLA+EAA     
Sbjct: 241 RASAYEEAVRKIKENTWQLTKRQLWKIGRLRKAGWDLKRVDATAAVVAVLAAEAARNGQK 300

Query: 301 --RSEIW 303
             R+ +W
Sbjct: 301 YGRNRLW 307

[Close]

BLAST of Lsi08G003900 vs. NCBI nr
Match: XP_022963367.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 542.3 bits (1396), Expect = 2.7e-150
Identity = 283/334 (84.73%), Postives = 297/334 (88.92%), Query Frame = 0


Query: 1   MTLSLPFSHS----------FPL----SRRPRCPLILSSVSSDSLLLLLGATGSGKSSLS 60
           MTLSLPFSHS          FPL    SRR + P +LSS+ SD+LLLLLGATGSGKSSLS
Sbjct: 1   MTLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRMLSSLPSDNLLLLLGATGSGKSSLS 60

Query: 61  IDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRS 120
           ID+AARFPSEIINSDKMQLYRGLD+TTNKLP S RR VPHHLLGDFDPLAGEVS AAFRS
Sbjct: 61  IDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFRS 120

Query: 121 HASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLW 180
            ASAVISGVVSRGNLP+VVGGSNS+IHALV A FD   DVF GP NRVSAEFRYRCCFLW
Sbjct: 121 QASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYRCCFLW 180

Query: 181 VDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRR 240
           VDVSFPVLK+YLS RVDDMLEAGMVGELAEFYDPEMA++EPR GLRKAIGVPEFEEFFRR
Sbjct: 181 VDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFRR 240

Query: 241 HHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAV 300
           +HPKGR+YREGDPLRA AYEEAVRKIKENTWQLTKRQL KIGRLR AGWDLKRVDATAAV
Sbjct: 241 YHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATAAV 300

Query: 301 VAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQE 321
           VAVLASEAAEKRSEIWE QVVEPSVKIVK FI+E
Sbjct: 301 VAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

[Close]

BLAST of Lsi08G003900 vs. NCBI nr
Match: KAG7032515.1 (hypothetical protein SDJN02_06564, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540.8 bits (1392), Expect = 7.9e-150
Identity = 282/334 (84.43%), Postives = 297/334 (88.92%), Query Frame = 0


Query: 1   MTLSLPFSHS----------FPL----SRRPRCPLILSSVSSDSLLLLLGATGSGKSSLS 60
           M+LSLPFSHS          FPL    SRR + P +LSS+ SD+LLLLLGATGSGKSSLS
Sbjct: 1   MSLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRMLSSLPSDNLLLLLGATGSGKSSLS 60

Query: 61  IDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRS 120
           ID+AARFPSEIINSDKMQLYRGLD+TTNKLP S RR VPHHLLGDFDPLAGEVS AAFRS
Sbjct: 61  IDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFRS 120

Query: 121 HASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLW 180
            ASAVISGVVSRGNLP+VVGGSNS+IHALV A FD   DVF GP NRVSAEFRYRCCFLW
Sbjct: 121 QASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYRCCFLW 180

Query: 181 VDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRR 240
           VDVSFPVLK+YLS RVDDMLEAGMVGELAEFYDPEMA++EPR GLRKAIGVPEFEEFFRR
Sbjct: 181 VDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFRR 240

Query: 241 HHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAV 300
           +HPKGR+YREGDPLRA AYEEAVRKIKENTWQLTKRQL KIGRLR AGWDLKRVDATAAV
Sbjct: 241 YHPKGREYREGDPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATAAV 300

Query: 301 VAVLASEAAEKRSEIWETQVVEPSVKIVKSFIQE 321
           VAVLASEAAEKRSEIWE QVVEPSVKIVK FI+E
Sbjct: 301 VAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

[Close]

BLAST of Lsi08G003900 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 306.2 bits (783), Expect = 3.1e-83
Identity = 162/315 (51.43%), Postives = 216/315 (68.57%), Query Frame = 0


Query: 10  SFPLSRRPRCPLI--------LSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSD 69
           SF   RR   PL+         S    D ++++LGATG+GKS LS+DLA RFPSEIINSD
Sbjct: 38  SFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSD 97

Query: 70  KMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNL 129
           K+Q+Y GL+ITTN++ L  RR VPHHLLG  +P  GE++   FRS AS V+  + SR  +
Sbjct: 98  KIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKV 157

Query: 130 PLVVGGSNSFIHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNR 189
           P++ GGSNSF+HAL+A  FD   D F      +S++ RY CCF+WVDVS  VL  YL  R
Sbjct: 158 PIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRR 217

Query: 190 VDDMLEAGMVGELAEFYDPEMADSEPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLR 249
           VD+M+++GM  EL+ FYDP  +  E R G+RKAIGVPEF+ +F+ + P+ +   + D LR
Sbjct: 218 VDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPE-KKMIKWDALR 277

Query: 250 ASAYEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKR-SE 309
            +AY++AV  IK NTW L KRQ+ KI  L+ AGW+++RVDATA+  AV+   ++EK+  E
Sbjct: 278 KAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRE 337

Query: 310 IWETQVVEPSVKIVK 316
            WE QV+EPSVKIVK
Sbjct: 338 NWEEQVLEPSVKIVK 351

[Close]

BLAST of Lsi08G003900 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-76
Identity = 155/299 (51.84%), Postives = 205/299 (68.56%), Query Frame = 0


Query: 29  DSLLLLLGATGSGKSSLSIDLAAR-FPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 88
           D ++L+ G TG+GKS LS+DLA R FP+EIINSDKMQ+Y+G +I TN +PL  +  VPHH
Sbjct: 45  DKVVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHH 104

Query: 89  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 148
           LLG F P  GE++PA FRS A+  IS ++S   LP+VVGGSNSF HAL+A  FD   D F
Sbjct: 105 LLGQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPF 164

Query: 149 --GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADS 208
             G   + + ++ RY+CC LWVDV  PVL ++L NRVD M+E+G+V +LAE YDP + DS
Sbjct: 165 SPGSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDS 224

Query: 209 EPRTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLW 268
             R G+RK IGV EF+ +F R +PK  D    D  R +AYEE V+ +KE T +L K+Q  
Sbjct: 225 GRRLGVRKTIGVEEFDRYF-RVYPKEMDKGIWDLARKAAYEETVKGMKERTCRLVKKQKE 284

Query: 269 KIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRS----EIWETQVVEPSVKIVKSFIQE 321
           KI +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK F+ E
Sbjct: 285 KIMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]

BLAST of Lsi08G003900 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 281.6 bits (719), Expect = 8.1e-76
Identity = 149/298 (50.00%), Postives = 204/298 (68.46%), Query Frame = 0


Query: 28  SDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHH 87
           +D +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  +  RR VPHH
Sbjct: 4   NDKMVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHH 63

Query: 88  LLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVF 147
           LLG+ +P AGEV+ A FR  A+  IS +  R  LP++ GGSNS+IHAL+A  +D     F
Sbjct: 64  LLGELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPF 123

Query: 148 GGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEP 207
              +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  EP
Sbjct: 124 SDHKGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP 183

Query: 208 RTGLRKAIGVPEFEEFFRRHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLWKI 267
             G+ KAIGV EF+++ +  +    D  + DP+R  AYE+AVR IKENT+QLTK Q+ KI
Sbjct: 184 -LGIWKAIGVQEFDDYLKM-YKWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKI 243

Query: 268 GRLRRAGWDLKRVDATA----AVVAVLASE-AAEKRSEIWETQVVEPSVKIVKSFIQE 321
            +LR AGWD+K+VDATA    A+ A    E  AE + +IW  +V+EP VKIV+S + +
Sbjct: 244 NKLRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]

BLAST of Lsi08G003900 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-73
Identity = 144/309 (46.60%), Postives = 198/309 (64.08%), Query Frame = 0


Query: 12  PLSRRPRCPLILSSVSSDSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDI 71
           P S  P   + +       +++++GATGSGKS LSIDLA RF  EI+NSDK+Q Y GL +
Sbjct: 26  PRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKV 85

Query: 72  TTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSF 131
           TTN++ +  R  VPHHLLG+  P   E++ + FRS AS  IS + +RGNLP++ GGSNSF
Sbjct: 86  TTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSF 145

Query: 132 IHALVAADFDATQDVFGGPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMV 191
           IHAL+   FD     F      +S+  RY CCFLWVDVS  VL  YLS RVD M+E+GM 
Sbjct: 146 IHALLVDRFDPKTYPFSS-ETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMF 205

Query: 192 GELAEFYDPEMADSEPRT-GLRKAIGVPEFEEFFRRHHPKGRD-YREGDPLRASAYEEAV 251
            ELA FYDP  + S  R  G+ K IG+PEF+ +F  + P+ +    E D  R  AY+EAV
Sbjct: 206 EELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAV 265

Query: 252 RKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEP 311
           ++IKENTW+L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ 
Sbjct: 266 QEIKENTWRLAKKQIERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDE 325

Query: 312 SVKIVKSFI 319
           S+K+VK F+
Sbjct: 326 SIKVVKRFL 325

[Close]

BLAST of Lsi08G003900 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 210.7 bits (535), Expect = 1.7e-54
Identity = 122/294 (41.50%), Postives = 167/294 (56.80%), Query Frame = 0


Query: 29  DSLLLLLGATGSGKSSLSIDLAARFPSEIINSDKMQLYRGLDITTNKLPLSHRRAVPHHL 88
           D ++ ++GATG+GKS L+IDLA RFP+EI+NSDK+Q+Y+GLDI TNK+       VPHHL
Sbjct: 33  DKVVFVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVYKGLDIVTNKVTPEESLGVPHHL 92

Query: 89  LGDFDPLAGEVSPAAFRSHASAVISGVVSRGNLPLVVGGSNSFIHALVAADFDATQDVFG 148
           LG       + +   F+  A   +  +V R  +P++ GGSNS+I ALV            
Sbjct: 93  LGTVHDTYEDFTAEDFQREAIRAVESIVQRDRVPIIAGGSNSYIEALV------------ 152

Query: 149 GPRNRVSAEFRYRCCFLWVDVSFPVLKRYLSNRVDDMLEAGMVGELAEFYDPEMADSEPR 208
              + V    RY CCFLWVDVS PVL  ++S RVD M++ G+V E+   +DP  +D    
Sbjct: 153 --NDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVRRIFDPSSSDYS-- 212

Query: 209 TGLRKAIGVPEFEEFFR---RHHPKGRDYREGDPLRASAYEEAVRKIKENTWQLTKRQLW 268
            G+R+AIGVPE +EF R   R++P     R          E A+ KIKENT  L  RQL 
Sbjct: 213 AGIRRAIGVPELDEFLRSEMRNYPAETTER--------LLETAIEKIKENTCLLACRQLQ 272

Query: 269 KIGRL-RRAGWDLKRVDATAAVVAVLASEAAEKRSEIWETQVVEPSVKIVKSFI 319
           KI RL ++  W++ RVDAT   +        E+  E W+  V  PS   V+ F+
Sbjct: 273 KIQRLYKQWKWNMHRVDATEVFL-----RRGEEADEAWDNSVAHPSALAVEKFL 297

The following BLAST results are available for this feature:

BLAST of Lsi08G003900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5GHF7	8.9e-88	58.80	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]
Q94ID3	4.3e-82	51.43	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9C6L1	1.7e-75	51.84	Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9SB60	1.1e-74	50.00	Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ...	[more]
Q9LJL4	1.8e-72	46.60	Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]

BLAST of Lsi08G003900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CF13	6.5e-166	93.13	Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BD73	6.5e-166	93.13	adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE...	[more]
A0A0A0K943	2.1e-164	92.81	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1	[more]
A0A6J1HF40	1.3e-150	84.73	adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463...	[more]
A0A6J1I7X6	1.5e-149	84.13	adenylate isopentenyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC11147045...	[more]

BLAST of Lsi08G003900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008445640.1	1.3e-165	93.13	PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 ad...	[more]
XP_004150914.1	4.3e-164	92.81	adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical prot...	[more]
XP_038886381.1	3.1e-154	91.21	adenylate isopentenyltransferase 1, chloroplastic-like [Benincasa hispida]	[more]
XP_022963367.1	2.7e-150	84.73	adenylate isopentenyltransferase-like [Cucurbita moschata]	[more]
KAG7032515.1	7.9e-150	84.43	hypothetical protein SDJN02_06564, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lsi08G003900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68460.1	3.1e-83	51.43	isopentenyltransferase 1	[more]
AT1G25410.1	1.2e-76	51.84	isopentenyltransferase 6	[more]
AT4G24650.1	8.1e-76	50.00	isopentenyltransferase 4	[more]
AT3G19160.1	1.3e-73	46.60	ATP/ADP isopentenyltransferases	[more]
AT5G19040.1	1.7e-54	41.50	isopentenyltransferase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01715	IPPT	coord: 64..138 e-value: 7.1E-16 score: 58.6 coord: 156..264 e-value: 2.6E-12 score: 47.0
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 171..277 e-value: 1.0E-121 score: 407.3
None	No IPR available	PANTHER	PTHR11088:SF65	ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATED	coord: 24..319
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 30..318 e-value: 1.0E-121 score: 407.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 30..201
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 24..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G003900.1	Lsi08G003900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity