Lsi08G003880 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G003880
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGTP cyclohydrolase II
Locationchr08: 10715414 .. 10719904 (-)
RNA-Seq ExpressionLsi08G003880
SyntenyLsi08G003880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATATTTGTATTAGAAAGAAAAAGAAAAATAAGAAGTAAAGACGTCCCTTCCTCTTTCTCCGTTGGCGCACGTCCGAGCACCCACCGGCTGCTGAAGTTTTATATTATTCTCTTTAAATCAAATCCCATCTCTTATTGAATTTCTTCTATCTTATCTTCCTCCCTGTGTGGCCGTTACCGATCGGCGGCACTCTCACTTTCTGCTGCAGTCATCTATCAATTGGTTGGAATCTGCCTGGGTTAGTTCATTTTTCTTTTTTCTTGTTTTGTTTCTCTGTACAACACAATAGGGTAAATTTGGAATTGATTTTAAATCCTGCTGATGTACTTTGGGTGGGATTTTTTTTGGGGATCAAAACAAGATTTCAAATTCTAGTTTTTGAATTCGTTCTAAAACATTGGAAATGCTGATTTGAAATCCTTCCAGTCCAGGGGTTCCAAGCACAGGGAAGAGGTTTCAGTACCAATGGCTTTTAGAAGCGTCTCGTTTCCATCTACAGCTCCCATTTCTCATGTTCAGTAAGCTCCCATTTTTCCATCTGGGTTTGTCTTTAATTCTTTAATTCTTTCGTTTTTGTCTTCGATTTATCTAGGGTTAACTTCATTTATCATTTTTGTGCAACTTTGATTTGTTTGATTTTGCTTCTTGGGTGGTGGATAATCCAACTTGCGTATGGTTAAATTTTTTGAAGAATGATCAACTGATAATGTGTATGTGTGTGTGGTTTTTTTTTTAAGTAATCTTTATGACTTAAAAGGATGGATCATGTTTGATGATGAAATTTCTCTGTTTATTTGTTTGAATCTTACTTCAAAAGTTCATAGAATTCTATGATCATCTGAGGCCCTTCTTTACTTCTCTGGTTGAGGGCCCGCAATTTTCAGGCGTCCATTAGGAGACTTATACATGTCACCTTCCATTTTATTACCAACTGTTTTGGAGAAATGCGGGCAGAACATCATGAATGACAGAGAAGATTCTTTATTGATAAAAAAAAATAATTATGTTATGCATTGGATCTCTTGGTCCCATTTGAATTAACGATTGTTATTTGAATCTAACAGGCTTATGTTTTCTCTTAGAGTTCAATGATCCAGTTTAGCTTAAAAAAATTCTCCCCATTCTACAATAAACTGCAGCTAGCTATTAAGTTTTTACTTATGAAGTTTAAGAACATTGATTGGAACTTATTCCTTGTCCCTCTTTTAGGCGTGGCTTTAGAGTTTATACCTTCTGTTTCTAGTTGGCATTTTTTAAAAAAGTAAATGAAGTATGCTTGATGTGTTCCACAATTTTCCTCATCTGCTAATGTCTTTTATGTTATTCTCTAAACATCTTGTGTATTTTTGTTTTATGTAGAAGATTCAAAGACTTGCAGTCTTTTGGTGGAATCAACTACGTTAAACCATACACGTCAACTGGGCTTGTTTCTGAGTTACCCTCAAACTGCAAAGCTAGTAAACTATTTTTGAGCACCAGGGGTTCAGTCCAAACGAGAGCTACAGTGATATCTGGAGAAGGAGGAGTTCTGTCTTCTTCCAATGGAAGTGCTGTAGTTGCGAAGGATATCATCCTTTGCAATCAGTCAGATTCAGCTTCTATAAGTAAGAGTTTTCCCATTGATGAAGATGAATATGATTTTGATCGCCCAACGGAAGGTTTTTCTTCTGTTGCAGATGCCATTAAAGATATTCGTCAGGGAAAGGTTGTCCATTATTCTAGCTTTTCTCTTTCTTCAACTTTTTGGCAAGTCATTTGACATGGGAGCATTTATTTTTCTACCTTGATTTTCACTTCTTCATCTTAGTAACAAGGATAGTGATGTTGCAGATGGTGCTTGTCGTTGATGATGAAGATAGAGAAAATGAGGGAGACTTGATAATGGCAGCACAAGCTGCAACTCCGGAGGCCATGGCTTTCATTGTAAAGTATGGAACAGGTATTGTTTGTGTGGCCATGAAAGGTGAAGACCTAGAGAGGTTACAGATTCCACTTATGGTGAACGAAAAGGAGAACGAGGAGAAACTTTGTACTGCTTTTACGGTGACAGTGGTATGAATTCTTCACTTGACATTTTGGATTATCATTCTATTTAGTAAATATAGTCGGTAATCCCTGTAAGAGTGTTCGTAGTTACAGCTATGGACACTTTTTTCTGCTTAATATTTTTCAACCAATTTGTTCCTGTAGCATGGTAATTTTGTATAGATATAATGCAATTTTTGACAATTGTTTCTTCAGGATGCAAGAGAAGGTACAACCACTGGAGTCTCAGCTAGTGATAGGGCAGCAACAGTATTGGCACTTGCATCAAGAGATTCAAAACCTTATGATTTTAAGCGACCAGGCCACATATTTCCCCTCAAGTACAGGGAAGGTGGTGTTTTGAAGAGAGCTGGGCATACTGAAGCATCTGTCGATCTTGCGGTGCTTGCTGGATTAGACCCAGTTGGAATCCTTTGTGAGGTAGTTGATGAAGACGGTTCAATGGCTAGGTTGCCAAAACTTCGCGAATTTGCCAAACAAGAAAACTTGAAAATCATTTCCATTGCTGATTTAGTCAGGTAAATTGATATCTTTCTTGCTAAAGTTTTAATTGTGTTAGCTCTTCATACATTTAGTTTTCACCTATATCTTGAAATACTCTTCATGTGAAAGCAAGTTTCTTGCTTGGATAATGACTACAACTTCATTTCAGCTTACAACAAAACAATCAATGATGTATTCTTAGTTATATATTTCATTTCTCGTCACCATGTTTAGTTCTAATAAATGATGGTATTGACAGGTATAGGAGGAAGAGAGATAGATTAGTTGAATGTGCCGGAGAAGCTGCTCGCATGCCGACTACATGGGGACCATTTCAGGCATATTGCTACAGATCTATTTTGGATGGCATTGAGCATATTGCAATGGTTAAGGTAACTAACTGCTTTTGTTTCAAAATGCAAAGCTTTGTAAAAGCATTTGCTATTACTGGTGCAAGTGCAACTACAAACGCTTTTGATAGTGTTACATGATGACCGTGCCTCAGACTATCTGAAGAAGTTATTTTATCCGAGTTTCTCTTGAGATGGTCTACCTTTTAGTTCCTAAATTCTGCCGTGTACTGCGTGCGAGTGTCTGTCGATGCATGGTGTGCTCTATTAGATGAAATTTCAGTGTCATAATGAGATCAATACTTAATTTTTGGACTACTTGCTTTGTGCAGGGTGATATTGGTGATGGACAAGATATTCTGGTGAGGGTACACTCGGAATGCCTCACCGGAGATATTTTTGGATCAGCTAGATGCGACTGTGGAAACCAGCTTGCACTAGCAATGAAGATGATCGAGGCAGAAGGCAGGGGTGTCTTGGTATATCTTCGTGGACATGAGGGACGGGGCATAGGTTTGGGCCACAAACTTCGTGCATATAACTTGCAAGATGCTGGGCGGGATACTGTCGAAGCCAATGAGGATCTGGGATTACCCGTGGATTCTCGCGAATATGGCATTGGTGCACAGGTACATATAGATTTACGCTAAACGACTAAAACAATTTTTTATTAGATCACACGAGTACATTCTTTTTTTAAAAAACAAAATCAAATCTCTCTTTGTTGCTTGCAGATTCTAAGGGATCTTGGGGTGAGAACAATGAAGCTAATGACAAACAACCCAACAAAGTACAGAGGCCTAAAGGGGTATGGCTTAGAAGTTTCAGGTAGAGTTCCCTTGATAACCCCAATTACAAAGGACAACAAGAGATACTTAGAGACAAAGCGGGCCAAAATGGGGCACGTCTACAACTTGGATTTTGGTGGTAGATTTAGAAATCGGATCGACGAGCATGAAACATCGAACGGTAGTGTTGCATCAAGCGATGCGATGGCTTGATCTAGCAATGTCGAATATTAAAGAGGGAAAAAAAAAAAAAACAAGTTGATAACCAAAGGTGTTGGAAATGAAATGACGCTGCTGCAGCTACTGGCTGACAATTAAGAAAAAAGCCTAACTTTGTACTGAAAACCATATTATAGTTATTATTTCATTCGGTTTAATTTGTTAGGTTTATTGTTTGATTGTAATCCACAATGGGGATGAAGTTTCAGACATTAGAACAATTGATGATCTGTGGAATGAATTTACAGTGCTACTCTAAGAAAGAAGCACGGATATGGGACACGACACAATACACATGCGTTGGAGCATGATTTTTTTTTTCTAATAATGTTAAAAAGTTTAAAAAAACTTATTATTATGTAATAGTTTTGAAACTAATGGTTGAAATATTGTAGTTTTTAAACCAGTCATCTCTCGCTTACGTGAAGAATATAAAAACGCATTTTATTTGTTTATTTACAATTTCTCTGGTTTTTCTCTCTCAGTTAATTTGTTAGGCGATGATGTGTGAATGATGCATCTGTTACATCACAGTGATGAGTAGAGTGTACTTCACTGTTTGTATTTTTCTTGTGAATCACTCTGTATTTATGGTCGTTGGTAG

mRNA sequence

TATATTTGTATTAGAAAGAAAAAGAAAAATAAGAAGTAAAGACGTCCCTTCCTCTTTCTCCGTTGGCGCACGTCCGAGCACCCACCGGCTGCTGAAGTTTTATATTATTCTCTTTAAATCAAATCCCATCTCTTATTGAATTTCTTCTATCTTATCTTCCTCCCTGTGTGGCCGTTACCGATCGGCGGCACTCTCACTTTCTGCTGCAGTCATCTATCAATTGGTTGGAATCTGCCTGGGGGTTCCAAGCACAGGGAAGAGGTTTCAGTACCAATGGCTTTTAGAAGCGTCTCGTTTCCATCTACAGCTCCCATTTCTCATGTTCAAAGATTCAAAGACTTGCAGTCTTTTGGTGGAATCAACTACGTTAAACCATACACGTCAACTGGGCTTGTTTCTGAGTTACCCTCAAACTGCAAAGCTAGTAAACTATTTTTGAGCACCAGGGGTTCAGTCCAAACGAGAGCTACAGTGATATCTGGAGAAGGAGGAGTTCTGTCTTCTTCCAATGGAAGTGCTGTAGTTGCGAAGGATATCATCCTTTGCAATCAGTCAGATTCAGCTTCTATAAGTAAGAGTTTTCCCATTGATGAAGATGAATATGATTTTGATCGCCCAACGGAAGGTTTTTCTTCTGTTGCAGATGCCATTAAAGATATTCGTCAGGGAAAGATGGTGCTTGTCGTTGATGATGAAGATAGAGAAAATGAGGGAGACTTGATAATGGCAGCACAAGCTGCAACTCCGGAGGCCATGGCTTTCATTGTAAAGTATGGAACAGGTATTGTTTGTGTGGCCATGAAAGGTGAAGACCTAGAGAGGTTACAGATTCCACTTATGGTGAACGAAAAGGAGAACGAGGAGAAACTTTGTACTGCTTTTACGGTGACAGTGTCGGTAATCCCTGTAAGAGTGTTCGTAGTTACAGCTATGGACACTTTTTTCTGCTTAATATTTTTCAACCAATTTGTTCCTGATGCAAGAGAAGGTACAACCACTGGAGTCTCAGCTAGTGATAGGGCAGCAACAGTATTGGCACTTGCATCAAGAGATTCAAAACCTTATGATTTTAAGCGACCAGGCCACATATTTCCCCTCAAGTACAGGGAAGGTGGTGTTTTGAAGAGAGCTGGGCATACTGAAGCATCTGTCGATCTTGCGGTGCTTGCTGGATTAGACCCAGTTGGAATCCTTTGTGAGGTAGTTGATGAAGACGGTTCAATGGCTAGGTTGCCAAAACTTCGCGAATTTGCCAAACAAGAAAACTTGAAAATCATTTCCATTGCTGATTTAGTCAGGTATAGGAGGAAGAGAGATAGATTAGTTGAATGTGCCGGAGAAGCTGCTCGCATGCCGACTACATGGGGACCATTTCAGGCATATTGCTACAGATCTATTTTGGATGGCATTGAGCATATTGCAATGGTTAAGGTAACTAACTGCTTTTGTTTCAAAATGCAAAGCTTTGTAAAAGCATTTGCTATTACTGGTGCAAGTGCAACTACAAACGCTTTTGATAGTTTCCTAAATTCTGCCGTGTACTGCGTGCGAGTGTCTGTCGATGCATGGTGTGCTCTATTAGATGAAATTTCAGGTGATATTGGTGATGGACAAGATATTCTGGTGAGGGTACACTCGGAATGCCTCACCGGAGATATTTTTGGATCAGCTAGATGCGACTGTGGAAACCAGCTTGCACTAGCAATGAAGATGATCGAGGCAGAAGGCAGGGGTGTCTTGGTATATCTTCGTGGACATGAGGGACGGGGCATAGGTTTGGGCCACAAACTTCGTGCATATAACTTGCAAGATGCTGGGCGGGATACTGTCGAAGCCAATGAGGATCTGGGATTACCCGTGGATTCTCGCGAATATGGCATTGGTGCACAGATTCTAAGGGATCTTGGGGTGAGAACAATGAAGCTAATGACAAACAACCCAACAAAGTACAGAGGCCTAAAGGGGTATGGCTTAGAAGTTTCAGGTAGAGTTCCCTTGATAACCCCAATTACAAAGGACAACAAGAGATACTTAGAGACAAAGCGGGCCAAAATGGGGCACGTCTACAACTTGGATTTTGGTGGTAGATTTAGAAATCGGATCGACGAGCATGAAACATCGAACGGTAGTGTTGCATCAAGCGATGCGATGGCTTGATCTAGCAATGTCGAATATTAAAGAGGGAAAAAAAAAAAAAACAAGTTGATAACCAAAGGTGTTGGAAATGAAATGACGCTGCTGCAGCTACTGGCTGACAATTAAGAAAAAAGCCTAACTTTGTACTGAAAACCATATTATAGTTATTATTTCATTCGGTTTAATTTGTTAGGTTTATTGTTTGATTGTAATCCACAATGGGGATGAAGTTTCAGACATTAGAACAATTGATGATCTGTGGAATGAATTTACAGTGCTACTCTAAGAAAGAAGCACGGATATGGGACACGACACAATACACATGCGTTGGAGCATGATTTTTTTTTTCTAATAATGTTAAAAAGTTTAAAAAAACTTATTATTATGTAATAGTTTTGAAACTAATGGTTGAAATATTGTAGTTTTTAAACCAGTCATCTCTCGCTTACGTGAAGAATATAAAAACGCATTTTATTTGTTTATTTACAATTTCTCTGGTTTTTCTCTCTCAGTTAATTTGTTAGGCGATGATGTGTGAATGATGCATCTGTTACATCACAGTGATGAGTAGAGTGTACTTCACTGTTTGTATTTTTCTTGTGAATCACTCTGTATTTATGGTCGTTGGTAG

Coding sequence (CDS)

ATGGCTTTTAGAAGCGTCTCGTTTCCATCTACAGCTCCCATTTCTCATGTTCAAAGATTCAAAGACTTGCAGTCTTTTGGTGGAATCAACTACGTTAAACCATACACGTCAACTGGGCTTGTTTCTGAGTTACCCTCAAACTGCAAAGCTAGTAAACTATTTTTGAGCACCAGGGGTTCAGTCCAAACGAGAGCTACAGTGATATCTGGAGAAGGAGGAGTTCTGTCTTCTTCCAATGGAAGTGCTGTAGTTGCGAAGGATATCATCCTTTGCAATCAGTCAGATTCAGCTTCTATAAGTAAGAGTTTTCCCATTGATGAAGATGAATATGATTTTGATCGCCCAACGGAAGGTTTTTCTTCTGTTGCAGATGCCATTAAAGATATTCGTCAGGGAAAGATGGTGCTTGTCGTTGATGATGAAGATAGAGAAAATGAGGGAGACTTGATAATGGCAGCACAAGCTGCAACTCCGGAGGCCATGGCTTTCATTGTAAAGTATGGAACAGGTATTGTTTGTGTGGCCATGAAAGGTGAAGACCTAGAGAGGTTACAGATTCCACTTATGGTGAACGAAAAGGAGAACGAGGAGAAACTTTGTACTGCTTTTACGGTGACAGTGTCGGTAATCCCTGTAAGAGTGTTCGTAGTTACAGCTATGGACACTTTTTTCTGCTTAATATTTTTCAACCAATTTGTTCCTGATGCAAGAGAAGGTACAACCACTGGAGTCTCAGCTAGTGATAGGGCAGCAACAGTATTGGCACTTGCATCAAGAGATTCAAAACCTTATGATTTTAAGCGACCAGGCCACATATTTCCCCTCAAGTACAGGGAAGGTGGTGTTTTGAAGAGAGCTGGGCATACTGAAGCATCTGTCGATCTTGCGGTGCTTGCTGGATTAGACCCAGTTGGAATCCTTTGTGAGGTAGTTGATGAAGACGGTTCAATGGCTAGGTTGCCAAAACTTCGCGAATTTGCCAAACAAGAAAACTTGAAAATCATTTCCATTGCTGATTTAGTCAGGTATAGGAGGAAGAGAGATAGATTAGTTGAATGTGCCGGAGAAGCTGCTCGCATGCCGACTACATGGGGACCATTTCAGGCATATTGCTACAGATCTATTTTGGATGGCATTGAGCATATTGCAATGGTTAAGGTAACTAACTGCTTTTGTTTCAAAATGCAAAGCTTTGTAAAAGCATTTGCTATTACTGGTGCAAGTGCAACTACAAACGCTTTTGATAGTTTCCTAAATTCTGCCGTGTACTGCGTGCGAGTGTCTGTCGATGCATGGTGTGCTCTATTAGATGAAATTTCAGGTGATATTGGTGATGGACAAGATATTCTGGTGAGGGTACACTCGGAATGCCTCACCGGAGATATTTTTGGATCAGCTAGATGCGACTGTGGAAACCAGCTTGCACTAGCAATGAAGATGATCGAGGCAGAAGGCAGGGGTGTCTTGGTATATCTTCGTGGACATGAGGGACGGGGCATAGGTTTGGGCCACAAACTTCGTGCATATAACTTGCAAGATGCTGGGCGGGATACTGTCGAAGCCAATGAGGATCTGGGATTACCCGTGGATTCTCGCGAATATGGCATTGGTGCACAGATTCTAAGGGATCTTGGGGTGAGAACAATGAAGCTAATGACAAACAACCCAACAAAGTACAGAGGCCTAAAGGGGTATGGCTTAGAAGTTTCAGGTAGAGTTCCCTTGATAACCCCAATTACAAAGGACAACAAGAGATACTTAGAGACAAAGCGGGCCAAAATGGGGCACGTCTACAACTTGGATTTTGGTGGTAGATTTAGAAATCGGATCGACGAGCATGAAACATCGAACGGTAGTGTTGCATCAAGCGATGCGATGGCTTGA

Protein sequence

MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGSVQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAGLDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNLDFGGRFRNRIDEHETSNGSVASSDAMA
Homology
BLAST of Lsi08G003880 vs. ExPASy Swiss-Prot
Match: P47924 (Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA1 PE=1 SV=2)

HSP 1 Score: 687.6 bits (1773), Expect = 1.3e-196
Identity = 368/590 (62.37%), Postives = 425/590 (72.03%), Query Frame = 0

Query: 44  LPSNCKASKLFLSTRGSVQTRATVISGEGGVLSSSNGSAVVAKDIILCN------QSDSA 103
           LPSN   S   + T  + + +A VIS E  +LS +NG+  ++   ++ +      ++DS 
Sbjct: 37  LPSNHPLSTFSIKT-NTGKVKAAVISREDDLLSFTNGNTPLSNGSLIDDRTEEPLEADSV 96

Query: 104 SI-----------SKSFPIDEDEYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENE 163
           S+           +  F  ++D+++ D PT GFSS+ +AI+DIRQGK+V+VVDDEDRENE
Sbjct: 97  SLGTLAADSAPAPANGFVAEDDDFELDLPTPGFSSIPEAIEDIRQGKLVVVVDDEDRENE 156

Query: 164 GDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVT 223
           GDL+MAAQ ATPEAMAFIV++GTGIVCV+MK +DLERL +PLMVN+KENEEKL TAFTVT
Sbjct: 157 GDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDDLERLHLPLMVNQKENEEKLSTAFTVT 216

Query: 224 VSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDF 283
           V                           DA+ GTTTGVSA DRA T+L+LASRDSKP DF
Sbjct: 217 V---------------------------DAKHGTTTGVSARDRATTILSLASRDSKPEDF 276

Query: 284 KRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAGLDPVGILCEVVDEDGSMARLPKLREF 343
            RPGHIFPLKYREGGVLKRAGHTEASVDL VLAGLDPVG+LCE+VD+DGSMARLPKLREF
Sbjct: 277 NRPGHIFPLKYREGGVLKRAGHTEASVDLTVLAGLDPVGVLCEIVDDDGSMARLPKLREF 336

Query: 344 AKQENLKIISIADLVRYRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVK 403
           A + NLK++SIADL+RYRRKRD+LVE A  AAR+PT WGPF AYCYRSILDGIEHIAMVK
Sbjct: 337 AAENNLKVVSIADLIRYRRKRDKLVERA-SAARIPTMWGPFTAYCYRSILDGIEHIAMVK 396

Query: 404 VTNCFCFKMQSFVKAFAITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDG 463
                                                                 G+IGDG
Sbjct: 397 ------------------------------------------------------GEIGDG 456

Query: 464 QDILVRVHSECLTGDIFGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKL 523
           QDILVRVHSECLTGDIFGSARCDCGNQLAL+M+ IEA GRGVLVYLRGHEGRGIGLGHKL
Sbjct: 457 QDILVRVHSECLTGDIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIGLGHKL 516

Query: 524 RAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYG 583
           RAYNLQDAGRDTVEANE+LGLPVDSREYGIGAQI+RDLGVRTMKLMTNNP KY GLKGYG
Sbjct: 517 RAYNLQDAGRDTVEANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVGLKGYG 543

Query: 584 LEVSGRVPLITPITKDNKRYLETKRAKMGHVYNLDFGGRFRNRIDEHETS 617
           L + GRVPL++ ITK+NKRYLETKR KMGH+Y L F G    +I+    S
Sbjct: 577 LAIVGRVPLLSLITKENKRYLETKRTKMGHMYGLKFKGDVVEKIESESES 543

BLAST of Lsi08G003880 vs. ExPASy Swiss-Prot
Match: Q0E079 (Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA2 PE=2 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 9.7e-187
Identity = 341/501 (68.06%), Postives = 374/501 (74.65%), Query Frame = 0

Query: 106 DED---EYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMA 165
           DED   E D D PTEGFSS+++AIKDI+QGK+V+VVDDE RENEGDLIMAA   TPEAMA
Sbjct: 120 DEDKDTEQDLDSPTEGFSSISEAIKDIQQGKLVIVVDDESRENEGDLIMAASLVTPEAMA 179

Query: 166 FIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDT 225
           F+V+YGTGIVCV+MK EDLERL +PLMV  KENEEKLCTAFTVTV               
Sbjct: 180 FVVRYGTGIVCVSMKEEDLERLNLPLMVATKENEEKLCTAFTVTV--------------- 239

Query: 226 FFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGV 285
                       DA+EGTTTGVSA DRA TV+ LAS DSKP DF RPGHIFPLKYREGGV
Sbjct: 240 ------------DAKEGTTTGVSAKDRAKTVMTLASPDSKPEDFNRPGHIFPLKYREGGV 299

Query: 286 LKRAGHTEASVDLAVLAGLDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVR 345
           LKRAGHTEASVDLA+LAGL P  +LCE+VDEDGSMARLPKLR FA++ENLKI+SIADL+R
Sbjct: 300 LKRAGHTEASVDLAMLAGLPPAAVLCEIVDEDGSMARLPKLRVFAERENLKIVSIADLIR 359

Query: 346 YRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAF 405
           YRRKRDRLVE     AR+P  WG  +AYCYRS++DGIEHIAMVK                
Sbjct: 360 YRRKRDRLVE-RSSVARLPLRWGNVRAYCYRSVIDGIEHIAMVK---------------- 419

Query: 406 AITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDI 465
                                                 G+IGDGQ +LVRVHSECLTGDI
Sbjct: 420 --------------------------------------GEIGDGQGVLVRVHSECLTGDI 479

Query: 466 FGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEAN 525
           FGSARCDCG+QLA+AM+MIE  GRGVLVYLRGHEGRGIGLGHKLRAYNLQD GRDTVEAN
Sbjct: 480 FGSARCDCGDQLAMAMEMIEKAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEAN 538

Query: 526 EDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKD 585
           EDLGLPVDSREYGIGAQILRDLGVR+MKLMTNNP KY GLKGYGL + GRVPL+TPIT +
Sbjct: 540 EDLGLPVDSREYGIGAQILRDLGVRSMKLMTNNPAKYGGLKGYGLSIVGRVPLVTPITSE 538

Query: 586 NKRYLETKRAKMGHVYNLDFG 604
           N+RYLETKR KMGHVY L  G
Sbjct: 600 NRRYLETKRTKMGHVYGLANG 538

BLAST of Lsi08G003880 vs. ExPASy Swiss-Prot
Match: Q6Z234 (Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA1 PE=2 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 7.6e-184
Identity = 349/558 (62.54%), Postives = 394/558 (70.61%), Query Frame = 0

Query: 44  LPSNCKASKLFLSTRGSVQTRATVISGEGGVLSSSNGSAVVAKDIILCN--QSDSASISK 103
           LP N +A    +S      +   +I+G+      ++ S VV  D        +D A +  
Sbjct: 39  LPYNHRARNFHISHAVGDSSEHVIINGQ------ASPSKVVQADAAALGTIAADMAPVVD 98

Query: 104 SFPIDEDEYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAM 163
            F  D+DE D D PTEGFSS+ +AI+DIRQGK V+VVDDEDRENEGDLIMAA   TPEAM
Sbjct: 99  GFSADDDELDLDSPTEGFSSIPEAIEDIRQGKYVIVVDDEDRENEGDLIMAASKVTPEAM 158

Query: 164 AFIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMD 223
           AFIV++GTGIVCV+MK +DLERL++PLMV  KENEEKL TAFTV+V              
Sbjct: 159 AFIVRHGTGIVCVSMKEDDLERLELPLMVTTKENEEKLRTAFTVSV-------------- 218

Query: 224 TFFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGG 283
                        DA+EGTTTGVSA DRA TVLALAS +SKP DF RPGHIFPLKYREGG
Sbjct: 219 -------------DAKEGTTTGVSAKDRANTVLALASPNSKPEDFNRPGHIFPLKYREGG 278

Query: 284 VLKRAGHTEASVDLAVLAGLDPVGILCEVV-DEDGSMARLPKLREFAKQENLKIISIADL 343
           VLKRAGHTEASVDLA+LAGL P  +LCE+V D+DGSMA LPKL++FA++ENLKIISIADL
Sbjct: 279 VLKRAGHTEASVDLAMLAGLPPAAVLCEIVDDDDGSMALLPKLQDFARRENLKIISIADL 338

Query: 344 VRYRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVK 403
           +RYRRKRDRLVE       +   WG FQ+YCYRS++DG+EHIAMVK              
Sbjct: 339 IRYRRKRDRLVERVC-VTPLQLQWGSFQSYCYRSLIDGMEHIAMVK-------------- 398

Query: 404 AFAITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTG 463
                                                   GD+GDGQDILVRVHSECLTG
Sbjct: 399 ----------------------------------------GDVGDGQDILVRVHSECLTG 458

Query: 464 DIFGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVE 523
           DIFGSARCDCGNQLALAM MIE  GRGV+VYLRGHEGRGIGLGHKLRAYNLQD GRDTVE
Sbjct: 459 DIFGSARCDCGNQLALAMTMIEKTGRGVVVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVE 508

Query: 524 ANEDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPIT 583
           ANEDLGLPVDSREYGIGAQILRDLGVRTM+LMTNNP KY GLKGYGL V GRVPL+TPIT
Sbjct: 519 ANEDLGLPVDSREYGIGAQILRDLGVRTMRLMTNNPAKYTGLKGYGLSVLGRVPLLTPIT 508

Query: 584 KDNKRYLETKRAKMGHVY 599
            +N+RY+ETKR KMGHVY
Sbjct: 579 NENRRYMETKRLKMGHVY 508

BLAST of Lsi08G003880 vs. ExPASy Swiss-Prot
Match: Q9FN89 (Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA3 PE=1 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 5.0e-151
Identity = 279/500 (55.80%), Postives = 349/500 (69.80%), Query Frame = 0

Query: 103 FPIDEDEYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMA 162
           F + + E D D PT+G+SS+  A++ +R+GK V+VVDDE  + EG+LIMAA   +P+ +A
Sbjct: 85  FFVSDAEGDPDCPTQGYSSIELALQALRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIA 144

Query: 163 FIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDT 222
           F++K G+GIV V MK E+LERL + LM  E E+E+     FT+TV               
Sbjct: 145 FLIKNGSGIVSVGMKKENLERLSLTLMSPEMEDEDSSAPTFTITV--------------- 204

Query: 223 FFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGV 282
                       DA+ GT+TGVSASDRA TVLAL+S D+KP DF+RPGH+FPLKYR+GGV
Sbjct: 205 ------------DAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVFPLKYRDGGV 264

Query: 283 LKRAGHTEASVDLAVLAGLDPVGILCEVVD-EDGSMARLPKLREFAKQENLKIISIADLV 342
           L+RAGHTEASVDL +LAGL P+ +L  ++D EDGSMA LP +++ A + ++ I+S+ DL+
Sbjct: 265 LRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEHDIPIVSLTDLI 324

Query: 343 RYRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKA 402
           RYRRKRD+LVE     +R+PT WG FQAYCYRS LDG E+IA+VK               
Sbjct: 325 RYRRKRDKLVERI-TVSRLPTKWGLFQAYCYRSKLDGTENIALVK--------------- 384

Query: 403 FAITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGD 462
                                                  G++G+G+DILVRVHSECLTGD
Sbjct: 385 ---------------------------------------GNVGNGEDILVRVHSECLTGD 444

Query: 463 IFGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEA 522
           IFGSARCDCGNQL LAM++IE EGRGV+VYLRGHEGRGIGLGHKLRAYNLQD G DTV+A
Sbjct: 445 IFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLRAYNLQDEGHDTVQA 502

Query: 523 NEDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITK 582
           N +LGL +DSREYGIGAQ+LRD+GVRTM+LMTNNP K+ GLKGYGL V GRVP++TPITK
Sbjct: 505 NVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGLAVVGRVPVVTPITK 502

Query: 583 DNKRYLETKRAKMGHVYNLD 602
           +N+RY+ETKR KMGH+Y  D
Sbjct: 565 ENRRYMETKRKKMGHIYISD 502

BLAST of Lsi08G003880 vs. ExPASy Swiss-Prot
Match: Q6L506 (Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA3 PE=2 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 9.4e-150
Identity = 297/566 (52.47%), Postives = 361/566 (63.78%), Query Frame = 0

Query: 58  RGSVQTRATVISGEGG---------------VLSSSNGSAVVAKDIILCNQSDSASISK- 117
           +GS++ R   I G GG               VL   NGS+   + +        A I++ 
Sbjct: 37  KGSLKLRCYAIGGLGGGENLNDPLKESNNGPVLQGFNGSSASFRTV-------GAKITQE 96

Query: 118 --SFPIDEDEYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPE 177
              F + + E D D+PT+GFSS+ +AI  + +GK V+ VDDE  +NEGDL+MAA  A PE
Sbjct: 97  TGDFFVSDAEGDPDKPTDGFSSIDEAIGALHEGKFVIAVDDESGDNEGDLVMAATLADPE 156

Query: 178 AMAFIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTA 237
           ++AF+++ G+GI+ V MK EDL RL IP+M    E E+    A TVTV            
Sbjct: 157 SIAFMIRNGSGIISVGMKEEDLTRLMIPMMSPIAEIEDISAAASTVTV------------ 216

Query: 238 MDTFFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYRE 297
                          DAR G +TGVSA+DRA T+  LAS DSKP D +RPGHIFPLKYR 
Sbjct: 217 ---------------DARVGISTGVSAADRAKTIFTLASPDSKPTDLRRPGHIFPLKYRN 276

Query: 298 GGVLKRAGHTEASVDLAVLAGLDPVGILCEVVDE-DGSMARLPKLREFAKQENLKIISIA 357
           GGVLKRAGHTEASVDL  LAGL PV +L  V++  DGSMA +P L++ A + ++ I+SIA
Sbjct: 277 GGVLKRAGHTEASVDLVALAGLRPVSVLSTVINPVDGSMAGMPVLKQMALEHDIPIVSIA 336

Query: 358 DLVRYRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSF 417
           DL+RYRRKR++LVE     +R+PT WG F+AYCY+S LDG EHIA+ K            
Sbjct: 337 DLIRYRRKREKLVELIA-VSRLPTKWGLFRAYCYQSKLDGTEHIAVAK------------ 396

Query: 418 VKAFAITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECL 477
                                                     GDIGDG+D+LVRVHSECL
Sbjct: 397 ------------------------------------------GDIGDGEDVLVRVHSECL 456

Query: 478 TGDIFGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDT 537
           TGDI GSARCDCGNQL LAM++I+  GRGVLVYLRGHEGRGIGLG KLRAYNLQD G DT
Sbjct: 457 TGDILGSARCDCGNQLDLAMQLIDKAGRGVLVYLRGHEGRGIGLGQKLRAYNLQDDGHDT 513

Query: 538 VEANEDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITP 597
           V+AN +LGL VDSREYGIGAQILRD+GVRTM+LMTNNP K+ GLKGYGL V GRVP+I+P
Sbjct: 517 VQANVELGLAVDSREYGIGAQILRDMGVRTMRLMTNNPAKFVGLKGYGLAVVGRVPVISP 513

Query: 598 ITKDNKRYLETKRAKMGHVYNLDFGG 605
           ITK+N+RYLETKR KMGHVY  D  G
Sbjct: 577 ITKENQRYLETKRTKMGHVYGSDLPG 513

BLAST of Lsi08G003880 vs. ExPASy TrEMBL
Match: A0A0A0KC47 (GTP cyclohydrolase II OS=Cucumis sativus OX=3659 GN=Csa_6G029440 PE=3 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.6e-285
Identity = 518/627 (82.62%), Postives = 532/627 (84.85%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSFPSTAPISHVQRFKDLQSF GINYVKPYTSTGLV ELP NCKASKLFL TRGS
Sbjct: 1   MAFRSVSFPSTAPISHVQRFKDLQSFVGINYVKPYTSTGLVLELPPNCKASKLFLGTRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRATVISGEGG+LSSSNG+AVV  DI   NQ DSASISKSFPIDEDEYDFDRPTEGF+
Sbjct: 61  VQTRATVISGEGGILSSSNGNAVVVNDITSFNQPDSASISKSFPIDEDEYDFDRPTEGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+D+RQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 SVADAIEDVRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRAATVLALASR+SKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG
Sbjct: 241 TTGVSASDRAATVLALASRNSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           L PVGILCEVVDEDGSMARLPKLREFAK+EN+KIISIADLVRYRRKRDRLVECAG+AARM
Sbjct: 301 LAPVGILCEVVDEDGSMARLPKLREFAKRENIKIISIADLVRYRRKRDRLVECAGDAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PT WGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTKWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+M
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQM 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI
Sbjct: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LR LGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL
Sbjct: 541 LRGLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           +FGGR RNRIDEHETSNGSVASSDA+A
Sbjct: 601 EFGGRLRNRIDEHETSNGSVASSDAVA 546

BLAST of Lsi08G003880 vs. ExPASy TrEMBL
Match: A0A5D3D4N7 (GTP cyclohydrolase II OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5066G00100 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 6.7e-284
Identity = 515/627 (82.14%), Postives = 531/627 (84.69%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSFPSTAPISHVQRFKDLQSF GINYVKPYTSTGLV ELP NCKASKLFL  RGS
Sbjct: 1   MAFRSVSFPSTAPISHVQRFKDLQSFVGINYVKPYTSTGLVLELPPNCKASKLFLGIRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRATV+SGEGG+LSSSNG+AVV  DI   NQ DSASISKSFPIDEDEYDFDRPT+GF+
Sbjct: 61  VQTRATVVSGEGGILSSSNGNAVVVNDITSFNQPDSASISKSFPIDEDEYDFDRPTDGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+D+RQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 SVADAIEDVRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRAATVLALASR+SKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG
Sbjct: 241 TTGVSASDRAATVLALASRNSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           L PVGILCEVVDEDGSMARLPKLREFAK+EN+KIISIADLVRYRRKRDRLVECAG+AARM
Sbjct: 301 LAPVGILCEVVDEDGSMARLPKLREFAKRENIKIISIADLVRYRRKRDRLVECAGDAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PT WGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTKWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+M
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQM 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI
Sbjct: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LRDLGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL
Sbjct: 541 LRDLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           +FGGR RNRIDE ETSNGSVASSDA+A
Sbjct: 601 EFGGRLRNRIDERETSNGSVASSDAVA 546

BLAST of Lsi08G003880 vs. ExPASy TrEMBL
Match: A0A1S3BD69 (GTP cyclohydrolase II OS=Cucumis melo OX=3656 GN=LOC103488598 PE=3 SV=1)

HSP 1 Score: 986.1 bits (2548), Expect = 6.7e-284
Identity = 515/627 (82.14%), Postives = 531/627 (84.69%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSFPSTAPISHVQRFKDLQSF GINYVKPYTSTGLV ELP NCKASKLFL  RGS
Sbjct: 1   MAFRSVSFPSTAPISHVQRFKDLQSFVGINYVKPYTSTGLVLELPPNCKASKLFLGIRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRATV+SGEGG+LSSSNG+AVV  DI   NQ DSASISKSFPIDEDEYDFDRPT+GF+
Sbjct: 61  VQTRATVVSGEGGILSSSNGNAVVVNDITSFNQPDSASISKSFPIDEDEYDFDRPTDGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+D+RQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 SVADAIEDVRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRAATVLALASR+SKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG
Sbjct: 241 TTGVSASDRAATVLALASRNSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           L PVGILCEVVDEDGSMARLPKLREFAK+EN+KIISIADLVRYRRKRDRLVECAG+AARM
Sbjct: 301 LAPVGILCEVVDEDGSMARLPKLREFAKRENIKIISIADLVRYRRKRDRLVECAGDAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PT WGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTKWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+M
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQM 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI
Sbjct: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LRDLGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL
Sbjct: 541 LRDLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           +FGGR RNRIDE ETSNGSVASSDA+A
Sbjct: 601 EFGGRLRNRIDERETSNGSVASSDAVA 546

BLAST of Lsi08G003880 vs. ExPASy TrEMBL
Match: A0A6J1CIP4 (GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111011529 PE=3 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 3.7e-274
Identity = 503/629 (79.97%), Postives = 522/629 (82.99%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSF STAPISH+QRFKDLQSFGGIN++KPYT+TGLVSEL SNC ASKLFL TRGS
Sbjct: 1   MAFRSVSFHSTAPISHLQRFKDLQSFGGINHIKPYTATGLVSELASNCNASKLFLGTRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRA VI GEGG+LSSSNG+AVVA D IL NQ DSASIS SFP+DEDEYDFDRPTEGF+
Sbjct: 61  VQTRAAVIPGEGGILSSSNGNAVVANDTILENQPDSASISNSFPVDEDEYDFDRPTEGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+DIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED
Sbjct: 121 SVADAIEDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRA TVL LASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLA+LAG
Sbjct: 241 TTGVSASDRAITVLKLASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAMLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           LDPVGILCEVVDEDGSMARLPKLREFA++ENLKIISIADLVRYRRKRDRLVEC G AARM
Sbjct: 301 LDPVGILCEVVDEDGSMARLPKLREFAQRENLKIISIADLVRYRRKRDRLVECVGGAARM 360

Query: 361 PTTWGPFQAYCYRSILD-GIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLN 420
           PTTWGPFQAYCYRSILD GIEHIAMVK                                 
Sbjct: 361 PTTWGPFQAYCYRSILDNGIEHIAMVK--------------------------------- 420

Query: 421 SAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMK 480
                                GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+
Sbjct: 421 ---------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQ 480

Query: 481 MIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQ 540
           +IEA GRGVLVYLRGHEGRGIGLGHKLRAYNLQD GRDTVEANE+LGLPVDSREYGIGAQ
Sbjct: 481 LIEAAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDEGRDTVEANEELGLPVDSREYGIGAQ 540

Query: 541 ILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYN 600
           ILRDLGVRTMKLMTNNP KY GLKGYGL VSGRVPLITPITKDN+RYLETKRAKMGHVYN
Sbjct: 541 ILRDLGVRTMKLMTNNPKKYIGLKGYGLAVSGRVPLITPITKDNRRYLETKRAKMGHVYN 548

Query: 601 LDFGGRFRNRIDEHETSNGSV-ASSDAMA 628
           LD GGRFRN I+EHE SNG+  ASSDA+A
Sbjct: 601 LDIGGRFRNLIEEHEKSNGNASASSDAVA 548

BLAST of Lsi08G003880 vs. ExPASy TrEMBL
Match: A0A6J1FSY3 (GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111447858 PE=3 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 3.7e-274
Identity = 495/627 (78.95%), Postives = 522/627 (83.25%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFR+VSF ST PISH+QRFKDLQSFGGIN+VKPY +T LVSEL S+CKASKLFL TRGS
Sbjct: 1   MAFRTVSFQSTPPISHLQRFKDLQSFGGINHVKPYVATSLVSELTSSCKASKLFLGTRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQ RAT+ISGEGGVLSSSNG+AVV+ DI+L NQ DSAS   SFP+ EDEYDFDRPT GF+
Sbjct: 61  VQPRATLISGEGGVLSSSNGNAVVSNDIVLGNQLDSASTRNSFPVGEDEYDFDRPTAGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           +VADA++DIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 AVADAVEDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERL+IPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLRIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRA TVL LASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLA+LAG
Sbjct: 241 TTGVSASDRATTVLKLASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAMLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           L+PVGILCEVVDEDGSMARLPKLREFAK+ENLKIISIADLVRYRRKRDRLVECAG AARM
Sbjct: 301 LEPVGILCEVVDEDGSMARLPKLREFAKRENLKIISIADLVRYRRKRDRLVECAGAAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PTTWGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTTWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM++
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQL 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEA GRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANE+LGLPVDSREYGIGAQI
Sbjct: 481 IEAAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEELGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LRDLGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGH+YNL
Sbjct: 541 LRDLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHIYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           DFG RFRN ID+H+TSN S  SSDA+A
Sbjct: 601 DFGSRFRNLIDDHQTSNSSAESSDAVA 546

BLAST of Lsi08G003880 vs. NCBI nr
Match: XP_038885189.1 (bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1005.4 bits (2598), Expect = 2.2e-289
Identity = 528/627 (84.21%), Postives = 538/627 (85.81%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSFPSTAPISHVQRFKDLQSFG +NY KPYTSTGLVSELPSNCKASKLFL TRGS
Sbjct: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGVLNYFKPYTSTGLVSELPSNCKASKLFLGTRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRATVISGEGGVLSSSNG+AVVAKDIIL NQSDSASISKSFPIDEDEYDFDRPTEGF+
Sbjct: 61  VQTRATVISGEGGVLSSSNGNAVVAKDIILSNQSDSASISKSFPIDEDEYDFDRPTEGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+DIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 SVADAIEDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMV+EKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVDEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRAATVLALASR+SKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG
Sbjct: 241 TTGVSASDRAATVLALASRNSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVE AG+AARM
Sbjct: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVESAGDAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PT WGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTKWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+M
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQM 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI
Sbjct: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LRDLGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL
Sbjct: 541 LRDLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           DFG RFRNRIDEHETSNGSVASSD MA
Sbjct: 601 DFGVRFRNRIDEHETSNGSVASSDVMA 546

BLAST of Lsi08G003880 vs. NCBI nr
Match: XP_004150928.1 (bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic [Cucumis sativus] >XP_011656512.1 bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic [Cucumis sativus] >KGN45942.1 hypothetical protein Csa_005146 [Cucumis sativus])

HSP 1 Score: 990.3 bits (2559), Expect = 7.4e-285
Identity = 518/627 (82.62%), Postives = 532/627 (84.85%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSFPSTAPISHVQRFKDLQSF GINYVKPYTSTGLV ELP NCKASKLFL TRGS
Sbjct: 1   MAFRSVSFPSTAPISHVQRFKDLQSFVGINYVKPYTSTGLVLELPPNCKASKLFLGTRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRATVISGEGG+LSSSNG+AVV  DI   NQ DSASISKSFPIDEDEYDFDRPTEGF+
Sbjct: 61  VQTRATVISGEGGILSSSNGNAVVVNDITSFNQPDSASISKSFPIDEDEYDFDRPTEGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+D+RQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 SVADAIEDVRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRAATVLALASR+SKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG
Sbjct: 241 TTGVSASDRAATVLALASRNSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           L PVGILCEVVDEDGSMARLPKLREFAK+EN+KIISIADLVRYRRKRDRLVECAG+AARM
Sbjct: 301 LAPVGILCEVVDEDGSMARLPKLREFAKRENIKIISIADLVRYRRKRDRLVECAGDAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PT WGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTKWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+M
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQM 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI
Sbjct: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LR LGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL
Sbjct: 541 LRGLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           +FGGR RNRIDEHETSNGSVASSDA+A
Sbjct: 601 EFGGRLRNRIDEHETSNGSVASSDAVA 546

BLAST of Lsi08G003880 vs. NCBI nr
Match: XP_008445639.1 (PREDICTED: bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-like [Cucumis melo] >KAA0049663.1 bifunctional riboflavin biosynthesis protein RIBA 1 [Cucumis melo var. makuwa] >TYK18509.1 bifunctional riboflavin biosynthesis protein RIBA 1 [Cucumis melo var. makuwa])

HSP 1 Score: 986.1 bits (2548), Expect = 1.4e-283
Identity = 515/627 (82.14%), Postives = 531/627 (84.69%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSFPSTAPISHVQRFKDLQSF GINYVKPYTSTGLV ELP NCKASKLFL  RGS
Sbjct: 1   MAFRSVSFPSTAPISHVQRFKDLQSFVGINYVKPYTSTGLVLELPPNCKASKLFLGIRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRATV+SGEGG+LSSSNG+AVV  DI   NQ DSASISKSFPIDEDEYDFDRPT+GF+
Sbjct: 61  VQTRATVVSGEGGILSSSNGNAVVVNDITSFNQPDSASISKSFPIDEDEYDFDRPTDGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+D+RQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 SVADAIEDVRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRAATVLALASR+SKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG
Sbjct: 241 TTGVSASDRAATVLALASRNSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           L PVGILCEVVDEDGSMARLPKLREFAK+EN+KIISIADLVRYRRKRDRLVECAG+AARM
Sbjct: 301 LAPVGILCEVVDEDGSMARLPKLREFAKRENIKIISIADLVRYRRKRDRLVECAGDAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PT WGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTKWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+M
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQM 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI
Sbjct: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LRDLGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL
Sbjct: 541 LRDLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           +FGGR RNRIDE ETSNGSVASSDA+A
Sbjct: 601 EFGGRLRNRIDERETSNGSVASSDAVA 546

BLAST of Lsi08G003880 vs. NCBI nr
Match: XP_022141022.1 (bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 953.7 bits (2464), Expect = 7.7e-274
Identity = 503/629 (79.97%), Postives = 522/629 (82.99%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFRSVSF STAPISH+QRFKDLQSFGGIN++KPYT+TGLVSEL SNC ASKLFL TRGS
Sbjct: 1   MAFRSVSFHSTAPISHLQRFKDLQSFGGINHIKPYTATGLVSELASNCNASKLFLGTRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQTRA VI GEGG+LSSSNG+AVVA D IL NQ DSASIS SFP+DEDEYDFDRPTEGF+
Sbjct: 61  VQTRAAVIPGEGGILSSSNGNAVVANDTILENQPDSASISNSFPVDEDEYDFDRPTEGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           SVADAI+DIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED
Sbjct: 121 SVADAIEDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERLQIPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLQIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRA TVL LASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLA+LAG
Sbjct: 241 TTGVSASDRAITVLKLASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAMLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           LDPVGILCEVVDEDGSMARLPKLREFA++ENLKIISIADLVRYRRKRDRLVEC G AARM
Sbjct: 301 LDPVGILCEVVDEDGSMARLPKLREFAQRENLKIISIADLVRYRRKRDRLVECVGGAARM 360

Query: 361 PTTWGPFQAYCYRSILD-GIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLN 420
           PTTWGPFQAYCYRSILD GIEHIAMVK                                 
Sbjct: 361 PTTWGPFQAYCYRSILDNGIEHIAMVK--------------------------------- 420

Query: 421 SAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMK 480
                                GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM+
Sbjct: 421 ---------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQ 480

Query: 481 MIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQ 540
           +IEA GRGVLVYLRGHEGRGIGLGHKLRAYNLQD GRDTVEANE+LGLPVDSREYGIGAQ
Sbjct: 481 LIEAAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDEGRDTVEANEELGLPVDSREYGIGAQ 540

Query: 541 ILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYN 600
           ILRDLGVRTMKLMTNNP KY GLKGYGL VSGRVPLITPITKDN+RYLETKRAKMGHVYN
Sbjct: 541 ILRDLGVRTMKLMTNNPKKYIGLKGYGLAVSGRVPLITPITKDNRRYLETKRAKMGHVYN 548

Query: 601 LDFGGRFRNRIDEHETSNGSV-ASSDAMA 628
           LD GGRFRN I+EHE SNG+  ASSDA+A
Sbjct: 601 LDIGGRFRNLIEEHEKSNGNASASSDAVA 548

BLAST of Lsi08G003880 vs. NCBI nr
Match: XP_022942989.1 (bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6600242.1 Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 953.7 bits (2464), Expect = 7.7e-274
Identity = 495/627 (78.95%), Postives = 522/627 (83.25%), Query Frame = 0

Query: 1   MAFRSVSFPSTAPISHVQRFKDLQSFGGINYVKPYTSTGLVSELPSNCKASKLFLSTRGS 60
           MAFR+VSF ST PISH+QRFKDLQSFGGIN+VKPY +T LVSEL S+CKASKLFL TRGS
Sbjct: 1   MAFRTVSFQSTPPISHLQRFKDLQSFGGINHVKPYVATSLVSELTSSCKASKLFLGTRGS 60

Query: 61  VQTRATVISGEGGVLSSSNGSAVVAKDIILCNQSDSASISKSFPIDEDEYDFDRPTEGFS 120
           VQ RAT+ISGEGGVLSSSNG+AVV+ DI+L NQ DSAS   SFP+ EDEYDFDRPT GF+
Sbjct: 61  VQPRATLISGEGGVLSSSNGNAVVSNDIVLGNQLDSASTRNSFPVGEDEYDFDRPTAGFA 120

Query: 121 SVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGED 180
           +VADA++DIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVK+GTGIVCVAMKGED
Sbjct: 121 AVADAVEDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKHGTGIVCVAMKGED 180

Query: 181 LERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGT 240
           LERL+IPLMVNEKENEEKLCTAFTVTV                           DAREGT
Sbjct: 181 LERLRIPLMVNEKENEEKLCTAFTVTV---------------------------DAREGT 240

Query: 241 TTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAG 300
           TTGVSASDRA TVL LASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLA+LAG
Sbjct: 241 TTGVSASDRATTVLKLASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAMLAG 300

Query: 301 LDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARM 360
           L+PVGILCEVVDEDGSMARLPKLREFAK+ENLKIISIADLVRYRRKRDRLVECAG AARM
Sbjct: 301 LEPVGILCEVVDEDGSMARLPKLREFAKRENLKIISIADLVRYRRKRDRLVECAGAAARM 360

Query: 361 PTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNS 420
           PTTWGPFQAYCYRSILDGIEHIAMVK                                  
Sbjct: 361 PTTWGPFQAYCYRSILDGIEHIAMVK---------------------------------- 420

Query: 421 AVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKM 480
                               GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAM++
Sbjct: 421 --------------------GDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMQL 480

Query: 481 IEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQI 540
           IEA GRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANE+LGLPVDSREYGIGAQI
Sbjct: 481 IEAAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEELGLPVDSREYGIGAQI 540

Query: 541 LRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNL 600
           LRDLGVRTMKLMTNNPTKY+GLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGH+YNL
Sbjct: 541 LRDLGVRTMKLMTNNPTKYKGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHIYNL 546

Query: 601 DFGGRFRNRIDEHETSNGSVASSDAMA 628
           DFG RFRN ID+H+TSN S  SSDA+A
Sbjct: 601 DFGSRFRNLIDDHQTSNSSAESSDAVA 546

BLAST of Lsi08G003880 vs. TAIR 10
Match: AT5G64300.1 (GTP cyclohydrolase II )

HSP 1 Score: 687.6 bits (1773), Expect = 9.5e-198
Identity = 368/590 (62.37%), Postives = 425/590 (72.03%), Query Frame = 0

Query: 44  LPSNCKASKLFLSTRGSVQTRATVISGEGGVLSSSNGSAVVAKDIILCN------QSDSA 103
           LPSN   S   + T  + + +A VIS E  +LS +NG+  ++   ++ +      ++DS 
Sbjct: 37  LPSNHPLSTFSIKT-NTGKVKAAVISREDDLLSFTNGNTPLSNGSLIDDRTEEPLEADSV 96

Query: 104 SI-----------SKSFPIDEDEYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENE 163
           S+           +  F  ++D+++ D PT GFSS+ +AI+DIRQGK+V+VVDDEDRENE
Sbjct: 97  SLGTLAADSAPAPANGFVAEDDDFELDLPTPGFSSIPEAIEDIRQGKLVVVVDDEDRENE 156

Query: 164 GDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVT 223
           GDL+MAAQ ATPEAMAFIV++GTGIVCV+MK +DLERL +PLMVN+KENEEKL TAFTVT
Sbjct: 157 GDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDDLERLHLPLMVNQKENEEKLSTAFTVT 216

Query: 224 VSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDF 283
           V                           DA+ GTTTGVSA DRA T+L+LASRDSKP DF
Sbjct: 217 V---------------------------DAKHGTTTGVSARDRATTILSLASRDSKPEDF 276

Query: 284 KRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAGLDPVGILCEVVDEDGSMARLPKLREF 343
            RPGHIFPLKYREGGVLKRAGHTEASVDL VLAGLDPVG+LCE+VD+DGSMARLPKLREF
Sbjct: 277 NRPGHIFPLKYREGGVLKRAGHTEASVDLTVLAGLDPVGVLCEIVDDDGSMARLPKLREF 336

Query: 344 AKQENLKIISIADLVRYRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVK 403
           A + NLK++SIADL+RYRRKRD+LVE A  AAR+PT WGPF AYCYRSILDGIEHIAMVK
Sbjct: 337 AAENNLKVVSIADLIRYRRKRDKLVERA-SAARIPTMWGPFTAYCYRSILDGIEHIAMVK 396

Query: 404 VTNCFCFKMQSFVKAFAITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDG 463
                                                                 G+IGDG
Sbjct: 397 ------------------------------------------------------GEIGDG 456

Query: 464 QDILVRVHSECLTGDIFGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKL 523
           QDILVRVHSECLTGDIFGSARCDCGNQLAL+M+ IEA GRGVLVYLRGHEGRGIGLGHKL
Sbjct: 457 QDILVRVHSECLTGDIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIGLGHKL 516

Query: 524 RAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYG 583
           RAYNLQDAGRDTVEANE+LGLPVDSREYGIGAQI+RDLGVRTMKLMTNNP KY GLKGYG
Sbjct: 517 RAYNLQDAGRDTVEANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVGLKGYG 543

Query: 584 LEVSGRVPLITPITKDNKRYLETKRAKMGHVYNLDFGGRFRNRIDEHETS 617
           L + GRVPL++ ITK+NKRYLETKR KMGH+Y L F G    +I+    S
Sbjct: 577 LAIVGRVPLLSLITKENKRYLETKRTKMGHMYGLKFKGDVVEKIESESES 543

BLAST of Lsi08G003880 vs. TAIR 10
Match: AT5G59750.1 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 536.2 bits (1380), Expect = 3.6e-152
Identity = 279/500 (55.80%), Postives = 349/500 (69.80%), Query Frame = 0

Query: 103 FPIDEDEYDFDRPTEGFSSVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMA 162
           F + + E D D PT+G+SS+  A++ +R+GK V+VVDDE  + EG+LIMAA   +P+ +A
Sbjct: 85  FFVSDAEGDPDCPTQGYSSIELALQALRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIA 144

Query: 163 FIVKYGTGIVCVAMKGEDLERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDT 222
           F++K G+GIV V MK E+LERL + LM  E E+E+     FT+TV               
Sbjct: 145 FLIKNGSGIVSVGMKKENLERLSLTLMSPEMEDEDSSAPTFTITV--------------- 204

Query: 223 FFCLIFFNQFVPDAREGTTTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGV 282
                       DA+ GT+TGVSASDRA TVLAL+S D+KP DF+RPGH+FPLKYR+GGV
Sbjct: 205 ------------DAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVFPLKYRDGGV 264

Query: 283 LKRAGHTEASVDLAVLAGLDPVGILCEVVD-EDGSMARLPKLREFAKQENLKIISIADLV 342
           L+RAGHTEASVDL +LAGL P+ +L  ++D EDGSMA LP +++ A + ++ I+S+ DL+
Sbjct: 265 LRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEHDIPIVSLTDLI 324

Query: 343 RYRRKRDRLVECAGEAARMPTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKA 402
           RYRRKRD+LVE     +R+PT WG FQAYCYRS LDG E+IA+VK               
Sbjct: 325 RYRRKRDKLVERI-TVSRLPTKWGLFQAYCYRSKLDGTENIALVK--------------- 384

Query: 403 FAITGASATTNAFDSFLNSAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGD 462
                                                  G++G+G+DILVRVHSECLTGD
Sbjct: 385 ---------------------------------------GNVGNGEDILVRVHSECLTGD 444

Query: 463 IFGSARCDCGNQLALAMKMIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEA 522
           IFGSARCDCGNQL LAM++IE EGRGV+VYLRGHEGRGIGLGHKLRAYNLQD G DTV+A
Sbjct: 445 IFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLRAYNLQDEGHDTVQA 502

Query: 523 NEDLGLPVDSREYGIGAQILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITK 582
           N +LGL +DSREYGIGAQ+LRD+GVRTM+LMTNNP K+ GLKGYGL V GRVP++TPITK
Sbjct: 505 NVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGLAVVGRVPVVTPITK 502

Query: 583 DNKRYLETKRAKMGHVYNLD 602
           +N+RY+ETKR KMGH+Y  D
Sbjct: 565 ENRRYMETKRKKMGHIYISD 502

BLAST of Lsi08G003880 vs. TAIR 10
Match: AT2G22450.1 (riboflavin biosynthesis protein, putative )

HSP 1 Score: 530.8 bits (1366), Expect = 1.5e-150
Identity = 291/522 (55.75%), Postives = 354/522 (67.82%), Query Frame = 0

Query: 64  RATVISGEGGVLSS--SNGSAV---VAKDIILCNQSDSASISKSF---PIDEDEYDFDRP 123
           R  VI  EG V SS  SNGS++   +  D++      +  I  +     ++++E+D DRP
Sbjct: 43  RMRVIPEEGDVFSSSKSNGSSMGIELQPDLVSFGTLAAEMIPTTMDSPEVEDEEFDLDRP 102

Query: 124 TEGFSSVADAIKDIRQGKMVLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVA 183
           T+GF+S+  AI+DIR GKMV+VVDDEDRENEGDLIMAA  ATPEAMAF+VK+GTGIVCV+
Sbjct: 103 TDGFASIPQAIEDIRHGKMVVVVDDEDRENEGDLIMAASLATPEAMAFVVKHGTGIVCVS 162

Query: 184 MKGEDLERLQIPLMVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPD 243
           MKGEDLERL++PLMV  K+NEEKL TAFTV+V                           D
Sbjct: 163 MKGEDLERLELPLMVTRKDNEEKLRTAFTVSV---------------------------D 222

Query: 244 AREGTTTGVSASDRAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDL 303
           A++GT+TGVSA DRA T+L LAS+DSKP DF RPGHIFPL+YREGGVLKRAGHTEASVDL
Sbjct: 223 AKKGTSTGVSARDRAQTILTLASKDSKPEDFNRPGHIFPLRYREGGVLKRAGHTEASVDL 282

Query: 304 AVLAGLDPVGILCEVVDEDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAG 363
            VLAGL+PV +LCE+VD+DGSMARLP+LR+FA++ NLK+ISIADL+RYRRKR+RLVE   
Sbjct: 283 TVLAGLEPVSVLCEIVDDDGSMARLPRLRQFAQENNLKLISIADLIRYRRKRERLVEFTA 342

Query: 364 EAARMPTTWGPFQAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFD 423
             A +PT WGPF+A+C++S+LDG+EHIAMVK                             
Sbjct: 343 -VAPIPTMWGPFKAHCFKSLLDGVEHIAMVK----------------------------- 402

Query: 424 SFLNSAVYCVRVSVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLA 483
                                    G+IGDG+DILVRVH+EC+T DIFG++    G QLA
Sbjct: 403 -------------------------GEIGDGKDILVRVHAECITDDIFGNS--SGGKQLA 462

Query: 484 LAMKMIEAEGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYG 543
           +AM++IE  GRGV VYLRG E +GI L HK R YN         +  E +  PV SREYG
Sbjct: 463 IAMRLIEENGRGVFVYLRGPESKGIDLSHKPRTYNTNS------DQAEGVSFPVASREYG 474

Query: 544 IGAQILRDLGVRTMKLMTNNPTKYRGLKGYGLEVSGRVPLIT 578
           IGAQILRDLGVR MK+MTNNP  Y GLKGYGL +SG+VPLIT
Sbjct: 523 IGAQILRDLGVREMKVMTNNPAHYVGLKGYGLSISGKVPLIT 474

BLAST of Lsi08G003880 vs. TAIR 10
Match: AT5G59750.2 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 519.2 bits (1336), Expect = 4.5e-147
Identity = 279/534 (52.25%), Postives = 350/534 (65.54%), Query Frame = 0

Query: 103 FPIDEDEYDFDRPTEGFSSVADAIKDIRQGKM---------------------------- 162
           F + + E D D PT+G+SS+  A++ +R+GK+                            
Sbjct: 85  FFVSDAEGDPDCPTQGYSSIELALQALRKGKVCSLTIFLKTSDYDDLFLQLYRFGLTKKL 144

Query: 163 ------VLVVDDEDRENEGDLIMAAQAATPEAMAFIVKYGTGIVCVAMKGEDLERLQIPL 222
                 V+VVDDE  + EG+LIMAA   +P+ +AF++K G+GIV V MK E+LERL + L
Sbjct: 145 SLMFQFVIVVDDETGDVEGNLIMAATLTSPKDIAFLIKNGSGIVSVGMKKENLERLSLTL 204

Query: 223 MVNEKENEEKLCTAFTVTVSVIPVRVFVVTAMDTFFCLIFFNQFVPDAREGTTTGVSASD 282
           M  E E+E+     FT+TV                           DA+ GT+TGVSASD
Sbjct: 205 MSPEMEDEDSSAPTFTITV---------------------------DAKSGTSTGVSASD 264

Query: 283 RAATVLALASRDSKPYDFKRPGHIFPLKYREGGVLKRAGHTEASVDLAVLAGLDPVGILC 342
           RA TVLAL+S D+KP DF+RPGH+FPLKYR+GGVL+RAGHTEASVDL +LAGL P+ +L 
Sbjct: 265 RAMTVLALSSLDAKPDDFRRPGHVFPLKYRDGGVLRRAGHTEASVDLMILAGLRPLSVLS 324

Query: 343 EVVD-EDGSMARLPKLREFAKQENLKIISIADLVRYRRKRDRLVECAGEAARMPTTWGPF 402
            ++D EDGSMA LP +++ A + ++ I+S+ DL+RYRRKRD+LVE     +R+PT WG F
Sbjct: 325 AILDQEDGSMASLPYMKKLATEHDIPIVSLTDLIRYRRKRDKLVERI-TVSRLPTKWGLF 384

Query: 403 QAYCYRSILDGIEHIAMVKVTNCFCFKMQSFVKAFAITGASATTNAFDSFLNSAVYCVRV 462
           QAYCYRS LDG E+IA+VK                                         
Sbjct: 385 QAYCYRSKLDGTENIALVK----------------------------------------- 444

Query: 463 SVDAWCALLDEISGDIGDGQDILVRVHSECLTGDIFGSARCDCGNQLALAMKMIEAEGRG 522
                        G++G+G+DILVRVHSECLTGDIFGSARCDCGNQL LAM++IE EGRG
Sbjct: 445 -------------GNVGNGEDILVRVHSECLTGDIFGSARCDCGNQLDLAMELIEKEGRG 504

Query: 523 VLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVEANEDLGLPVDSREYGIGAQILRDLGVR 582
           V+VYLRGHEGRGIGLGHKLRAYNLQD G DTV+AN +LGL +DSREYGIGAQ+LRD+GVR
Sbjct: 505 VVVYLRGHEGRGIGLGHKLRAYNLQDEGHDTVQANVELGLSIDSREYGIGAQMLRDIGVR 536

Query: 583 TMKLMTNNPTKYRGLKGYGLEVSGRVPLITPITKDNKRYLETKRAKMGHVYNLD 602
           TM+LMTNNP K+ GLKGYGL V GRVP++TPITK+N+RY+ETKR KMGH+Y  D
Sbjct: 565 TMRLMTNNPAKFTGLKGYGLAVVGRVPVVTPITKENRRYMETKRKKMGHIYISD 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P479241.3e-19662.37Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsi... [more]
Q0E0799.7e-18768.06Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=O... [more]
Q6Z2347.6e-18462.54Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=O... [more]
Q9FN895.0e-15155.80Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidop... [more]
Q6L5069.4e-15052.47Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS... [more]
Match NameE-valueIdentityDescription
A0A0A0KC473.6e-28582.62GTP cyclohydrolase II OS=Cucumis sativus OX=3659 GN=Csa_6G029440 PE=3 SV=1[more]
A0A5D3D4N76.7e-28482.14GTP cyclohydrolase II OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold50... [more]
A0A1S3BD696.7e-28482.14GTP cyclohydrolase II OS=Cucumis melo OX=3656 GN=LOC103488598 PE=3 SV=1[more]
A0A6J1CIP43.7e-27479.97GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111011529 PE=3 SV=1[more]
A0A6J1FSY33.7e-27478.95GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111447858 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038885189.12.2e-28984.21bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-like [Beninca... [more]
XP_004150928.17.4e-28582.62bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic [Cucumis sati... [more]
XP_008445639.11.4e-28382.14PREDICTED: bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-li... [more]
XP_022141022.17.7e-27479.97bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-like isoform ... [more]
XP_022942989.17.7e-27478.95bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic-like isoform ... [more]
Match NameE-valueIdentityDescription
AT5G64300.19.5e-19862.37GTP cyclohydrolase II [more]
AT5G59750.13.6e-15255.80DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II [more]
AT2G22450.11.5e-15055.75riboflavin biosynthesis protein, putative [more]
AT5G59750.24.5e-14752.25DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0004223,4-dihydroxy-2-butanone 4-phosphate synthase, RibBTIGRFAMTIGR00506TIGR00506coord: 119..344
e-value: 1.1E-68
score: 228.7
IPR0004223,4-dihydroxy-2-butanone 4-phosphate synthase, RibBPFAMPF00926DHBP_synthasecoord: 124..343
e-value: 1.7E-76
score: 256.1
IPR0004223,4-dihydroxy-2-butanone 4-phosphate synthase, RibBHAMAPMF_00180RibBcoord: 117..348
score: 37.03548
IPR036144GTP cyclohydrolase II superfamilyGENE3D3.40.50.10990GTP cyclohydrolase IIcoord: 353..577
e-value: 2.0E-70
score: 237.6
IPR036144GTP cyclohydrolase II superfamilySUPERFAMILY142695RibA-likecoord: 440..576
IPR000926GTP cyclohydrolase II, RibATIGRFAMTIGR00505TIGR00505coord: 440..597
e-value: 1.0E-64
score: 215.5
IPR000926GTP cyclohydrolase II, RibAHAMAPMF_00179RibAcoord: 346..600
score: 36.296432
IPR000926GTP cyclohydrolase II, RibACDDcd00641GTP_cyclohydro2coord: 350..596
e-value: 5.55131E-105
score: 313.667
NoneNo IPR availableGENE3D3.90.870.10DHBP synthasecoord: 108..351
e-value: 3.2E-86
score: 289.9
NoneNo IPR availablePANTHERPTHR21327:SF41BIFUNCTIONAL RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBA 1, CHLOROPLASTIC-LIKE ISOFORM X1coord: 422..605
coord: 32..386
NoneNo IPR availablePANTHERPTHR21327GTP CYCLOHYDROLASE II-RELATEDcoord: 422..605
coord: 32..386
IPR032677GTP cyclohydrolase IIPFAMPF00925GTP_cyclohydro2coord: 357..574
e-value: 7.0E-68
score: 227.4
IPR016299Riboflavin biosynthesis protein RibBAHAMAPMF_01283RibBAcoord: 118..596
score: 50.393452
IPR017945DHBP synthase RibB-like alpha/beta domain superfamilySUPERFAMILY55821YrdC/RibBcoord: 118..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G003880.1Lsi08G003880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009231 riboflavin biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0008686 3,4-dihydroxy-2-butanone-4-phosphate synthase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003935 GTP cyclohydrolase II activity
molecular_function GO:0046872 metal ion binding