Lsi08G003370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G003370
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	rRNA N-glycosidase
Location	chr08: 9166757 .. 9168589 (-)
RNA-Seq Expression	Lsi08G003370
Synteny	Lsi08G003370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTGCTGATAGAAGTCTATCAATATCTATTAATATTTTTTTTTGCTATTTTATGGAAATAGTTTGACATTTTTTTTATTTGTGAAAATTTTCCAAATTACTATGATATGCCATATCGACCTATAAAAATAGTTTAGTTTAGAGGCTCCAATGGAATCCAAATTGAGGGAATGGGAAAGATGAACAGATTTCCAGTGGTGTGTTTGTTAATTATTCTCAGCATCTTCCATGGAGTTCCTACTGCTGAAGGCGATGATAAGAATGTAATCTTCAGTTTGTCTGGTTCAGATTCCAAATCTTATCGCAAGTTCATTACGTCTCTGAGAAATGCTCTTCCAAATGCTGGTGAAGTCTACAAGATACCTTTATTGGTTTCATCTGCTTCTAGCTCAGGACGTTATATATTTATGCAACTCTCCAATTATGAGGAGAAAACCATCAAAGTGGCTATTGATGTAACCAATGTTTACATTATGGGGTATGTTGTCAATACAACATCCTACTTTTTCAACGAGACCGACGCTCAATTAGCTTCTAAATTTGTATTCCCAGGTACTTAATTACACTATTACACTCTCGTATTCTGGTGTTGGAATGTCTTTAATCTGTTATTTAACCTAAGTTGTATTCGTAATTTGTCATATTTACGTTATTTATGATTTTGATTTTAGAGTTTAGAGGAGTGCGTTATATAAACTCTCTAACCCTAAAAGCGTATTACTTTTGTTTGTAGACGTAGCTAACACACTGTTAGTAGACCACGTAAATCTATGTGTCTGGTTTACGTTTTTTGTTAATTACTTTTTATTGGCAATTTCTTAACATCTAAACTTTTATTCCAAGGTACTTAAACTGGTACGCTCCCATATTCTATGTTGGAATGTCATGAATATGTGATTTAATCAAAGTTGTATTCTTATTTATCATACTTATGTTATTTACGATTTTGGCCTTAGGGTATAGAGGTTTGCGCTATATAAACTCTCTATTTCTAGAGCCGTCTTGTCTTTGCCCATAGACGTAGCTAACACACTATTAGTAGACCACATAAATCTATGTGTTGATTCTCTATTATTTACGTTTTTTGTTTATCTTAATTTCTATTCTAAGCTACTTAGACTATTACACTCCCATATTATGACGTTAGGAAAGCCTTAAATCTCAATTATCCTATTTATGTTATTTGCAATTTTGGCCTTAGGGTTTAGAGAAATATGCTATATAAAGTCTCTTATCTCTAGAGGTGTCTTTCGTTTGCTTGTGGACATAGCTAACACACTGTCAGTAAACCACATAAATCTATGTGTCAATTGTCTATTATTTATGTTTTTTTGTTTATCTTTTATTGCCGATTTAAGATTCTAAATTTGTATTTGAAGGTACAAAAACTGTTACACTCCCATATTCTGGGAATTACCAAAAGCTTCAAAATGCTGCAGGCAAAGAAAGAGACTCGATTCCTCTTGGATTCTTGGCTTTGGACAGTGCAATTTCAAGCTTGTATTATTATGATGCCAAAGCTGCTCCTGCGGCATTTCTCGTACTCATTCAGTCCACGGCCGAGGCTTCAAGATTTAAATATATTGAGCAACAAATTATCGACAGAATTAGCAAAAACGTGGTGCCAAGTCAAGCAGCTATAAGTTTGGAAAACGAATGGTCTCTTCTCTCCAAACAAATTCAGATGGCACAATCTAACAACGGACAGTTTCAAACTCCCATTAAGCTTGTTAACGATAAAGGCCTTACAACACAAGTTACCAACGTTAGTTCTCCGGTTGTCACCAAGAACATCAAGTTGCTGCTAAACAAGCAAAATATTGCAGCATTTTGA

mRNA sequence

GTTGCTGATAGAAGTCTATCAATATCTATTAATATTTTTTTTTGCTATTTTATGGAAATAGTTTGACATTTTTTTTATTTGTGAAAATTTTCCAAATTACTATGATATGCCATATCGACCTATAAAAATAGTTTAGTTTAGAGGCTCCAATGGAATCCAAATTGAGGGAATGGGAAAGATGAACAGATTTCCAGTGGTGTGTTTGTTAATTATTCTCAGCATCTTCCATGGAGTTCCTACTGCTGAAGGCGATGATAAGAATGTAATCTTCAGTTTGTCTGGTTCAGATTCCAAATCTTATCGCAAGTTCATTACGTCTCTGAGAAATGCTCTTCCAAATGCTGGTGAAGTCTACAAGATACCTTTATTGGTTTCATCTGCTTCTAGCTCAGGACGTTATATATTTATGCAACTCTCCAATTATGAGGAGAAAACCATCAAAGTGGCTATTGATGTAACCAATGTTTACATTATGGGGTATGTTGTCAATACAACATCCTACTTTTTCAACGAGACCGACGCTCAATTAGCTTCTAAATTTGTATTCCCAGGCAAAGAAAGAGACTCGATTCCTCTTGGATTCTTGGCTTTGGACAGTGCAATTTCAAGCTTGTATTATTATGATGCCAAAGCTGCTCCTGCGGCATTTCTCGTACTCATTCAGTCCACGGCCGAGGCTTCAAGATTTAAATATATTGAGCAACAAATTATCGACAGAATTAGCAAAAACGTGGTGCCAAGTCAAGCAGCTATAAGTTTGGAAAACGAATGGTCTCTTCTCTCCAAACAAATTCAGATGGCACAATCTAACAACGGACAGTTTCAAACTCCCATTAAGCTTGTTAACGATAAAGGCCTTACAACACAAGTTACCAACGTTAGTTCTCCGGTTGTCACCAAGAACATCAAGTTGCTGCTAAACAAGCAAAATATTGCAGCATTTTGA

Coding sequence (CDS)

ATGGGAAAGATGAACAGATTTCCAGTGGTGTGTTTGTTAATTATTCTCAGCATCTTCCATGGAGTTCCTACTGCTGAAGGCGATGATAAGAATGTAATCTTCAGTTTGTCTGGTTCAGATTCCAAATCTTATCGCAAGTTCATTACGTCTCTGAGAAATGCTCTTCCAAATGCTGGTGAAGTCTACAAGATACCTTTATTGGTTTCATCTGCTTCTAGCTCAGGACGTTATATATTTATGCAACTCTCCAATTATGAGGAGAAAACCATCAAAGTGGCTATTGATGTAACCAATGTTTACATTATGGGGTATGTTGTCAATACAACATCCTACTTTTTCAACGAGACCGACGCTCAATTAGCTTCTAAATTTGTATTCCCAGGCAAAGAAAGAGACTCGATTCCTCTTGGATTCTTGGCTTTGGACAGTGCAATTTCAAGCTTGTATTATTATGATGCCAAAGCTGCTCCTGCGGCATTTCTCGTACTCATTCAGTCCACGGCCGAGGCTTCAAGATTTAAATATATTGAGCAACAAATTATCGACAGAATTAGCAAAAACGTGGTGCCAAGTCAAGCAGCTATAAGTTTGGAAAACGAATGGTCTCTTCTCTCCAAACAAATTCAGATGGCACAATCTAACAACGGACAGTTTCAAACTCCCATTAAGCTTGTTAACGATAAAGGCCTTACAACACAAGTTACCAACGTTAGTTCTCCGGTTGTCACCAAGAACATCAAGTTGCTGCTAAACAAGCAAAATATTGCAGCATTTTGA

Protein sequence

MGKMNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYKIPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASKFVFPGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVLIQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIKLVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF

Homology

[Close]

BLAST of Lsi08G003370 vs. ExPASy Swiss-Prot
Match: Q9FRX4 (Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 1.7e-100
Identity = 198/275 (72.00%), Postives = 225/275 (81.82%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNRF V+  L+ILSIFHGVPTAEG D  V FSL GS+ KSY KFITS+RNALPNAG++Y 
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEG-DVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLLV S S S RYI MQLSNYE  TI +A+DVTNVYIMGY+VN TSYFFNETDAQLASK
Sbjct: 61  IPLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASK 120

Query: 124 FVFPG--------------------KERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           FVF G                    KERDSIPLGF+ALDSAIS+LYYYD+++AP AFLVL
Sbjct: 121 FVFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVL 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEA+R+KYIE+QIIDRIS + VP  AAISLENEWSLLSKQIQ+A+SNNGQFQTP+K
Sbjct: 181 IQTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVK 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF 259
           ++NDKG+ T+VTNVSS VVTKNI LLLNK NIA+F
Sbjct: 241 IINDKGILTEVTNVSSLVVTKNIMLLLNKLNIASF 274

[Close]

BLAST of Lsi08G003370 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 2.7e-79
Identity = 170/276 (61.59%), Postives = 203/276 (73.55%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           M RF V    +IL+IF    T E D   V FSLSGS S SY KFI  LR ALP+ G VY 
Sbjct: 1   MKRFTV----LILAIFVAASTVEAD---VRFSLSGSSSTSYSKFIGDLRKALPSNGTVYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           I LL+SSAS + RY  M LSNY+ K I VA+DVTNVYIMGY+VN+TSYFFNE+DA+LAS+
Sbjct: 61  ITLLLSSASGASRYTLMTLSNYDGKAITVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQ 120

Query: 124 FVF--------------------PGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           +VF                     GK R+ IPLGF ALDSAI++L++YD+ AA AAFLV+
Sbjct: 121 YVFKGSTIVTLPYSGNYEKLQTAAGKIREKIPLGFPALDSAITTLFHYDSTAAAAAFLVI 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEASRFKYIE QII+RISKN VPS A ISLENEWS LSKQIQ+AQ+NNG F+TP+ 
Sbjct: 181 IQTTAEASRFKYIEGQIIERISKNQVPSLATISLENEWSALSKQIQLAQTNNGTFKTPVV 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLN-KQNIAAF 259
           + +DKG   ++TNV+S VVTKNI+LLLN KQN+AAF
Sbjct: 241 ITDDKGQRVEITNVTSKVVTKNIQLLLNYKQNVAAF 269

[Close]

BLAST of Lsi08G003370 vs. ExPASy Swiss-Prot
Match: P22851 (Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.7e-73
Identity = 154/249 (61.85%), Postives = 192/249 (77.11%), Query Frame = 0


Query: 31  NVIFSLSGSDSKSYRKFITSLRNALPNAGEVYKIPLLVSSASSSGRYIFMQLSNYEEKTI 90
           NV FSLSG+DSKSY KFIT+LR ALP+  +V  IPLL+ SAS + RYI MQLSNY+ K I
Sbjct: 2   NVSFSLSGADSKSYSKFITALRKALPSKEKVSNIPLLLPSASGASRYILMQLSNYDAKAI 61

Query: 91  KVAIDVTNVYIMGYVVNTTSYFFNETDAQLASKFVF--------------------PGKE 150
            +AIDVTNVYIMGY+VN+TSYF NE+DA+LAS++VF                     GK 
Sbjct: 62  TMAIDVTNVYIMGYLVNSTSYFANESDAKLASQYVFKGSTLVTIPYSGNYERLQNAAGKI 121

Query: 151 RDSIPLGFLALDSAISSLYYYDAKAAPAAFLVLIQSTAEASRFKYIEQQIIDRISKNVVP 210
           R+ IPLGF ALDSA++S+++YD+ AA AAFLV++Q+TAEASRFKYIE QII+RI KN VP
Sbjct: 122 REKIPLGFRALDSALTSIFHYDSTAAAAAFLVILQTTAEASRFKYIEGQIIERIPKNEVP 181

Query: 211 SQAAISLENE-WSLLSKQIQMAQSNNGQFQTPIKLVNDKGLTTQVTNVSSPVVTK--NIK 257
           S AA+SLENE WSLLSKQIQ+AQ+NNG F+TP+ ++++KG   ++TN++S V  K  N K
Sbjct: 182 SPAALSLENEAWSLLSKQIQLAQTNNGAFRTPVVIIDNKGQRVEITNLASKVQIKDVNSK 241

[Close]

BLAST of Lsi08G003370 vs. ExPASy Swiss-Prot
Match: P16094 (Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 4.7e-71
Identity = 157/274 (57.30%), Postives = 192/274 (70.07%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           M+RF V+  L IL+IF G    +GD   V F LSG+D +SY  FI  LRNALP   +VY 
Sbjct: 1   MSRFSVLSFL-ILAIFLGGSIVKGD---VSFRLSGADPRSYGMFIKDLRNALPFREKVYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLL+ S S +GRY+ M L NY+ KTI VA+DVTNVYIMGY+ +TTSYFFNE  A+LAS+
Sbjct: 61  IPLLLPSVSGAGRYLLMHLFNYDGKTITVAVDVTNVYIMGYLADTTSYFFNEPAAELASQ 120

Query: 124 FVF---------------------PGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLV 183
           +VF                      GK R+ IP+G  ALDSAIS+L +YD+ AA  A LV
Sbjct: 121 YVFRDARRKITLPYSGNYERLQIAAGKPREKIPIGLPALDSAISTLLHYDSTAAAGALLV 180

Query: 184 LIQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPI 243
           LIQ+TAEA+RFKYIEQQI +R  ++ VPS A ISLEN WS LSKQIQ+AQ NNG F+TPI
Sbjct: 181 LIQTTAEAARFKYIEQQIQERAYRDEVPSLATISLENSWSGLSKQIQLAQGNNGIFRTPI 240

Query: 244 KLVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIA 257
            LV++KG   Q+TNV+S VVT NI+LLLN +NIA
Sbjct: 241 VLVDNKGNRVQITNVTSKVVTSNIQLLLNTRNIA 270

[Close]

BLAST of Lsi08G003370 vs. ExPASy Swiss-Prot
Match: P84530 (Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 4.7e-71
Identity = 150/241 (62.24%), Postives = 182/241 (75.52%), Query Frame = 0


Query: 31  NVIFSLSGSDSKSYRKFITSLRNALPNAGEVYKIPLLVSSASSSGRYIFMQLSNYEEKTI 90
           +V FSLSGS S SY KFI +LR ALP+ G VY I LL+SSAS + RY  M+LSNY+ K I
Sbjct: 1   DVSFSLSGSSSTSYSKFIGALRKALPSNGTVYNITLLLSSASGASRYTLMKLSNYDGKAI 60

Query: 91  KVAIDVTNVYIMGYVVNTTSYFFNETDAQLASKFVF--------------------PGKE 150
            VAIDVTNVYIMGY+VN+TSYFFNE+DA+LAS++VF                     GK 
Sbjct: 61  TVAIDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFAGSTIVTLPYSGNYEKLQTAAGKI 120

Query: 151 RDSIPLGFLALDSAISSLYYYDAKAAPAAFLVLIQSTAEASRFKYIEQQIIDRISKNVVP 210
           R+ IPLGF ALDSAI++L++YD+ AA AAFLV+IQ+TAE+SRFKYIE QII RISKN VP
Sbjct: 121 REKIPLGFPALDSAITTLFHYDSTAAAAAFLVIIQTTAESSRFKYIEGQIIMRISKNGVP 180

Query: 211 SQAAISLENEWSLLSKQIQMAQSNNGQFQTPIKLVNDKGLTTQVTNVSSPVVTKNIKLLL 252
           S A ISLENEWS LSKQIQ+AQ+NNG F+TP+ +++  G   ++ NV S VVTKNI+LLL
Sbjct: 181 SLATISLENEWSALSKQIQLAQTNNGTFKTPVVIMDAGGQRVEIGNVGSKVVTKNIQLLL 240

[Close]

BLAST of Lsi08G003370 vs. ExPASy TrEMBL
Match: A0A6J1GMJ7 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 9.1e-94
Identity = 197/273 (72.16%), Postives = 219/273 (80.22%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNR  V+ LL IL+IFH   TA GD  N+ FSLSGS SKSY KFITSLRNALPNAG+VY 
Sbjct: 1   MNRLSVLSLL-ILTIFHEGHTAVGD--NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLL+ +   S RY  M+LSNYEEKTI VAIDVTNVYIMGY+VNTTSYFFNE  AQLAS+
Sbjct: 61  IPLLLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASE 120

Query: 124 FVFPG--------------------KERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           FVF G                    K+RDSIPLGFLALD+AIS+LY+YD KAAPAAFLVL
Sbjct: 121 FVFQGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVL 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEASRFKYIE+QIIDRI +N VPS AAISLENEWSLLSKQIQ+A SNNG FQTP+K
Sbjct: 181 IQTTAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVK 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIA 257
           L+NDKGL+ +VTNVSS VVT NIKLLLNKQN+A
Sbjct: 241 LINDKGLSVEVTNVSSLVVTNNIKLLLNKQNVA 270

[Close]

BLAST of Lsi08G003370 vs. ExPASy TrEMBL
Match: B0EVM6 (rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.2e-79
Identity = 171/275 (62.18%), Postives = 207/275 (75.27%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNRF  + LLIIL  F    T EG   NV FSLSG+DSKSY KFIT+LR ALP+  +V  
Sbjct: 1   MNRFTFLSLLIILIAFF---TVEG--ANVSFSLSGADSKSYSKFITALRKALPSKEKVSN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLL+ SAS + RYI MQLSNY+ K I +AIDVTNVYIMGY+VN+TSYFFNE+DA+LAS+
Sbjct: 61  IPLLLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQ 120

Query: 124 FVF--------------------PGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           +VF                     GK R+ IPLGF A DSAI+SL++YD+ AA  AFLV+
Sbjct: 121 YVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVI 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEASRFKYIE QII+RI KN VPS AA+SLENEWS LSKQIQ+AQ+NNG F+TP+ 
Sbjct: 181 IQTTAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVV 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF 259
           ++++KG   ++ +V+S VVT NIKLLLNKQNIAAF
Sbjct: 241 IIDNKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAF 270

[Close]

BLAST of Lsi08G003370 vs. ExPASy TrEMBL
Match: Q00980 (rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.2e-79
Identity = 171/275 (62.18%), Postives = 207/275 (75.27%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNRF  + LLIIL  F    T EG   NV FSLSG+DSKSY KFIT+LR ALP+  +V  
Sbjct: 1   MNRFTFLSLLIILIAFF---TVEG--ANVSFSLSGADSKSYSKFITALRKALPSKEKVSN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLL+ SAS + RYI MQLSNY+ K I +AIDVTNVYIMGY+VN+TSYFFNE+DA+LAS+
Sbjct: 61  IPLLLPSASGASRYILMQLSNYDAKAITMAIDVTNVYIMGYLVNSTSYFFNESDAKLASQ 120

Query: 124 FVF--------------------PGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           +VF                     GK R+ IPLGF A DSAI+SL++YD+ AA  AFLV+
Sbjct: 121 YVFKGSTIVTLPYSGNYERLQNAAGKVREKIPLGFRAFDSAITSLFHYDSTAAAGAFLVI 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEASRFKYIE QII+RI KN VPS AA+SLENEWS LSKQIQ+AQ+NNG F+TP+ 
Sbjct: 181 IQTTAEASRFKYIEGQIIERIPKNEVPSPAALSLENEWSALSKQIQLAQTNNGAFRTPVV 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF 259
           ++++KG   ++ +V+S VVT NIKLLLNKQNIAAF
Sbjct: 241 IIDNKGQRVEIKDVNSKVVTNNIKLLLNKQNIAAF 270

[Close]

BLAST of Lsi08G003370 vs. ExPASy TrEMBL
Match: A0A6J1JVG7 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.0e-73
Identity = 161/275 (58.55%), Postives = 202/275 (73.45%), Query Frame = 0


Query: 3   KMNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVY 62
           KMN   V C L+IL+IFHG  +    D  V F+L GS+SKSY  FI +LR+ALP+  +VY
Sbjct: 10  KMNVVSVFC-LVILAIFHGSSSCVAGD--VSFNLLGSNSKSYSNFIKALRSALPSKDKVY 69

Query: 63  KIPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLAS 122
            I LL+ SA+ + RY  ++LSNY+ K I VAIDVTNVYIMGY+VN+TSYFFNE+DAQLAS
Sbjct: 70  NISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLAS 129

Query: 123 KFVF--------------------PGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLV 182
           K+VF                     GK+R+ IPLGF ALDSAI++L++YD+ AA AAF+V
Sbjct: 130 KYVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDSAITTLFHYDSTAAAAAFIV 189

Query: 183 LIQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPI 242
           +IQ TAEASR+KYIE Q+I RISK+ VPS A ISLEN WS LSKQIQ+AQ+N G F+ P+
Sbjct: 190 VIQCTAEASRYKYIEAQMIKRISKDDVPSPAIISLENSWSALSKQIQLAQTNKGVFKAPV 249

Query: 243 KLVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAA 258
            + +D+G   ++TNVSS VVTKNI+LLLN QNIAA
Sbjct: 250 LITDDQGQRVEITNVSSNVVTKNIQLLLNSQNIAA 281

[Close]

BLAST of Lsi08G003370 vs. ExPASy TrEMBL
Match: A0A6J1GMM2 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 2.2e-71
Identity = 155/274 (56.57%), Postives = 200/274 (72.99%), Query Frame = 0


Query: 3   KMNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVY 62
           +MN   V C L+IL+IFHG  +    D  V F+L GS+SKSY  FI +LR+ALP+  +VY
Sbjct: 10  RMNSVSVFC-LVILAIFHGSSSCVAGD--VSFNLLGSNSKSYSNFIEALRSALPSKDKVY 69

Query: 63  KIPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLAS 122
            I LL+ SA+ + RY  ++LSNY+ K I VAIDVTNVYIMGY+VN+TSYFFNE+DAQLAS
Sbjct: 70  NISLLLGSATGASRYTQVKLSNYDGKAITVAIDVTNVYIMGYLVNSTSYFFNESDAQLAS 129

Query: 123 KFVF--------------------PGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLV 182
           K+VF                     GK+R+ IPLGF ALD+AI++L++YD+ AA AAF+V
Sbjct: 130 KYVFKDSTRITLPYSGNYEKLQNAAGKKREKIPLGFPALDNAITTLFHYDSTAAAAAFIV 189

Query: 183 LIQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPI 242
           +IQ TAEASR++YIE Q+I RISK+ VPS A ISLEN WS LSKQIQ+AQ+N G F+ P+
Sbjct: 190 VIQCTAEASRYRYIEAQMIKRISKDDVPSLAIISLENSWSALSKQIQLAQTNKGVFKAPV 249

Query: 243 KLVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIA 257
            + +D+G   ++TNVSS VV KNI+LLLN +NIA
Sbjct: 250 LITDDQGQRVEITNVSSKVVAKNIQLLLNSKNIA 280

[Close]

BLAST of Lsi08G003370 vs. NCBI nr
Match: XP_038884329.1 (putative ribosome-inactivating protein [Benincasa hispida])

HSP 1 Score: 367.5 bits (942), Expect = 9.7e-98
Identity = 199/275 (72.36%), Postives = 224/275 (81.45%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNRF VVCL+I+    HGVPTAEGD+ +VIF++ GS+SKSYR FITSLR ALPNAG+VY 
Sbjct: 1   MNRFSVVCLIILNIFVHGVPTAEGDN-DVIFNMLGSNSKSYRNFITSLRKALPNAGDVYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLLV S     RYI MQLSNYEEK+IKVA+DVTNVYIMGY+VN+TSYFF+ETDAQ ASK
Sbjct: 61  IPLLVPSLPGPRRYIVMQLSNYEEKSIKVAVDVTNVYIMGYLVNSTSYFFDETDAQTASK 120

Query: 124 FVFPG--------------------KERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           +VF                      K+RDSIPLGF ALDSAIS+LYYYDAKAAPAAFLVL
Sbjct: 121 YVFQNTKMITLPYSSNYQKLQNAARKDRDSIPLGFKALDSAISTLYYYDAKAAPAAFLVL 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQSTAEASRFKYIEQ IID IS+N +PS AAISLENEWSLLSKQIQ+A+SNNG FQ P++
Sbjct: 181 IQSTAEASRFKYIEQLIIDGISENKLPSLAAISLENEWSLLSKQIQLAKSNNGLFQIPVQ 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF 259
           L+ND+G   QVTNVSSPVVTKNIKLLLN QN+AAF
Sbjct: 241 LINDRGSPIQVTNVSSPVVTKNIKLLLNSQNVAAF 274

[Close]

BLAST of Lsi08G003370 vs. NCBI nr
Match: Q9FRX4.1 (RecName: Full=Putative ribosome-inactivating protein; AltName: Full=rRNA N-glycosidase; Flags: Precursor [Cucumis ficifolius] >BAB19677.1 unnamed protein product [Cucumis ficifolius])

HSP 1 Score: 367.1 bits (941), Expect = 1.3e-97
Identity = 198/275 (72.00%), Postives = 225/275 (81.82%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNRF V+  L+ILSIFHGVPTAEG D  V FSL GS+ KSY KFITS+RNALPNAG++Y 
Sbjct: 1   MNRFSVLMCLVILSIFHGVPTAEG-DVTVKFSLLGSNHKSYSKFITSMRNALPNAGDIYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLLV S S S RYI MQLSNYE  TI +A+DVTNVYIMGY+VN TSYFFNETDAQLASK
Sbjct: 61  IPLLVPSISGSRRYILMQLSNYEGNTITMAVDVTNVYIMGYLVNGTSYFFNETDAQLASK 120

Query: 124 FVFPG--------------------KERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           FVF G                    KERDSIPLGF+ALDSAIS+LYYYD+++AP AFLVL
Sbjct: 121 FVFQGTKSITLPYSGNYQKLQSVARKERDSIPLGFMALDSAISTLYYYDSRSAPIAFLVL 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEA+R+KYIE+QIIDRIS + VP  AAISLENEWSLLSKQIQ+A+SNNGQFQTP+K
Sbjct: 181 IQTTAEAARYKYIEKQIIDRISVSKVPDLAAISLENEWSLLSKQIQIAKSNNGQFQTPVK 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF 259
           ++NDKG+ T+VTNVSS VVTKNI LLLNK NIA+F
Sbjct: 241 IINDKGILTEVTNVSSLVVTKNIMLLLNKLNIASF 274

[Close]

BLAST of Lsi08G003370 vs. NCBI nr
Match: XP_023547652.1 (putative ribosome-inactivating protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 353.2 bits (905), Expect = 1.9e-93
Identity = 197/275 (71.64%), Postives = 219/275 (79.64%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNR  V+ LL IL+IFH   TA GD  N+ FSLSGS SKSY KFITSLRNALPNAG+VY 
Sbjct: 1   MNRLSVLSLL-ILTIFHEGHTAVGD--NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLL+ +   S RY  M+LSNYEEKTI VAIDVTNVYIMGY+VNTTSYFFNE  AQLAS+
Sbjct: 61  IPLLLPTLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASE 120

Query: 124 FVF--------------------PGKERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           FVF                      K+RDSIPLGFLALD+AIS+LY+YD KAAPAAFLVL
Sbjct: 121 FVFQDTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVL 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEASRFKYIE+QIIDRI +N VPS AAISLENEWSLLSKQIQ+A SNNG FQTP+K
Sbjct: 181 IQTTAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVK 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF 259
           L+NDKGL+ +VTNVSS VVT NIKLLLNKQN+A F
Sbjct: 241 LINDKGLSVEVTNVSSLVVTNNIKLLLNKQNVATF 272

[Close]

BLAST of Lsi08G003370 vs. NCBI nr
Match: XP_022953187.1 (putative ribosome-inactivating protein [Cucurbita moschata])

HSP 1 Score: 353.2 bits (905), Expect = 1.9e-93
Identity = 197/273 (72.16%), Postives = 219/273 (80.22%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNR  V+ LL IL+IFH   TA GD  N+ FSLSGS SKSY KFITSLRNALPNAG+VY 
Sbjct: 1   MNRLSVLSLL-ILTIFHEGHTAVGD--NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLL+ +   S RY  M+LSNYEEKTI VAIDVTNVYIMGY+VNTTSYFFNE  AQLAS+
Sbjct: 61  IPLLLPNLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASE 120

Query: 124 FVFPG--------------------KERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           FVF G                    K+RDSIPLGFLALD+AIS+LY+YD KAAPAAFLVL
Sbjct: 121 FVFQGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVL 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEASRFKYIE+QIIDRI +N VPS AAISLENEWSLLSKQIQ+A SNNG FQTP+K
Sbjct: 181 IQTTAEASRFKYIEKQIIDRIGRNEVPSLAAISLENEWSLLSKQIQIANSNNGTFQTPVK 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIA 257
           L+NDKGL+ +VTNVSS VVT NIKLLLNKQN+A
Sbjct: 241 LINDKGLSVEVTNVSSLVVTNNIKLLLNKQNVA 270

[Close]

BLAST of Lsi08G003370 vs. NCBI nr
Match: KAG6575377.1 (hypothetical protein SDJN03_26016, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013918.1 hypothetical protein SDJN02_24087, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 342.4 bits (877), Expect = 3.3e-90
Identity = 195/275 (70.91%), Postives = 217/275 (78.91%), Query Frame = 0


Query: 4   MNRFPVVCLLIILSIFHGVPTAEGDDKNVIFSLSGSDSKSYRKFITSLRNALPNAGEVYK 63
           MNR  V+ LL IL+IFH   TA GD  N+ FSLSGS SKSY KFITSLRNALPNAG+VY 
Sbjct: 1   MNRLSVLSLL-ILTIFHEGHTAVGD--NLSFSLSGSSSKSYSKFITSLRNALPNAGKVYN 60

Query: 64  IPLLVSSASSSGRYIFMQLSNYEEKTIKVAIDVTNVYIMGYVVNTTSYFFNETDAQLASK 123
           IPLL+ +   S RY  M+LSNYEEKTI VAIDVTNVYIMGY+VNTTSYFFNE  AQLAS+
Sbjct: 61  IPLLLPTLKGSARYTLMKLSNYEEKTITVAIDVTNVYIMGYLVNTTSYFFNEPAAQLASE 120

Query: 124 FVFPG--------------------KERDSIPLGFLALDSAISSLYYYDAKAAPAAFLVL 183
           FVF G                    K+RDSIPLGFLALD+AIS+LY+YD KAAPAAFLVL
Sbjct: 121 FVFQGTKHILLPYSGNYQKLQIAADKDRDSIPLGFLALDTAISALYHYDTKAAPAAFLVL 180

Query: 184 IQSTAEASRFKYIEQQIIDRISKNVVPSQAAISLENEWSLLSKQIQMAQSNNGQFQTPIK 243
           IQ+TAEASRFKYIE+QIIDRI +N VPS AAISLE   SLLSKQIQ+A SNNG FQTP+K
Sbjct: 181 IQTTAEASRFKYIEKQIIDRIGRNEVPSLAAISLER--SLLSKQIQIANSNNGTFQTPVK 240

Query: 244 LVNDKGLTTQVTNVSSPVVTKNIKLLLNKQNIAAF 259
           L+NDKGL+ +VTNVSS VVT NIKLLLNKQN+A F
Sbjct: 241 LINDKGLSVEVTNVSSLVVTNNIKLLLNKQNVATF 270

The following BLAST results are available for this feature:

BLAST of Lsi08G003370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FRX4	1.7e-100	72.00	Putative ribosome-inactivating protein OS=Cucumis ficifolius OX=131071 PE=2 SV=1	[more]
Q00465	2.7e-79	61.59	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P22851	1.7e-73	61.85	Ribosome-inactivating protein luffin-B OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P16094	4.7e-71	57.30	Ribosome-inactivating protein momordin I OS=Momordica charantia OX=3673 PE=1 SV=...	[more]
P84530	4.7e-71	62.24	Ribosome-inactivating protein luffaculin 1 OS=Luffa acutangula OX=56866 PE=1 SV=...	[more]

BLAST of Lsi08G003370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMJ7	9.1e-94	72.16	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455802 PE=3 SV=1	[more]
B0EVM6	2.2e-79	62.18	rRNA N-glycosidase OS=Luffa acutangula OX=56866 GN=RIPI PE=2 SV=1	[more]
Q00980	2.2e-79	62.18	rRNA N-glycosidase OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A6J1JVG7	1.0e-73	58.55	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111488687 PE=3 SV=1	[more]
A0A6J1GMM2	2.2e-71	56.57	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111455894 PE=3 SV=1	[more]

BLAST of Lsi08G003370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884329.1	9.7e-98	72.36	putative ribosome-inactivating protein [Benincasa hispida]	[more]
Q9FRX4.1	1.3e-97	72.00	RecName: Full=Putative ribosome-inactivating protein; AltName: Full=rRNA N-glyco...	[more]
XP_023547652.1	1.9e-93	71.64	putative ribosome-inactivating protein [Cucurbita pepo subsp. pepo]	[more]
XP_022953187.1	1.9e-93	72.16	putative ribosome-inactivating protein [Cucurbita moschata]	[more]
KAG6575377.1	3.3e-90	70.91	hypothetical protein SDJN03_26016, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lsi08G003370 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 42..55 score: 45.11 coord: 192..209 score: 49.85 coord: 160..180 score: 59.4
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 31..129 e-value: 4.5E-23 score: 83.7
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 130..172 e-value: 1.0E-7 score: 34.1
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 173..256 e-value: 1.3E-28 score: 100.8
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 40..209 e-value: 2.8E-22 score: 79.3
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 35..254
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 35..254
IPR017988	Ribosome-inactivating protein conserved site	PROSITE	PS00275	SHIGA_RICIN	coord: 164..180
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 31..254

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G003370.1	Lsi08G003370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051607	defense response to virus
biological_process	GO:0035821	modulation of process of other organism
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0090729	toxin activity