Lsi08G003040 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi08G003040
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionethylene-responsive transcription factor CRF6-like
Locationchr08: 8049544 .. 8050284 (+)
RNA-Seq ExpressionLsi08G003040
SyntenyLsi08G003040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCAACTCACTCTCGCTTCAAGTTCCGTGAACGCCGCTCCGTCACCGGAATGCCTCTCCCGGCCGATTCCCCCGCCCCTAGGATCGTCCGCGTCTTCGTCACTGACTCTGACGCCACCGATACTTCCAGCAGCGACGACGACGACGAATTCGTCTGTCTCGTCTGCCGCCACATCAACGAGATACGCCTCCACGATTCTACGCACTCAAAGTCGAAGGAAGTCGACCCGATCAGAAAATTTCGCGGCGTGCGGCGGCGGCCGTGGGGGAAATTCGCGGCGGAGATTCGAGATCCGATTAAACGCACTAGAGTCTGGCTTGGAACCTTTGATTCGCCCGAGGAAGCCGCAATCGTCTACGATCAAGCAGCGATTCGATTCCGAGGTCCTGATGCCTTAACGAACATCGTCAAGCCGCCGCAACGGAATCCGCCGCCGGAGGAGATTTCTGATTCTTCTATGGAAATATCCGACGACCTCGCCGCCGTCTCCGGCGGAGGAATGCGTTCTTCTCCAACTTCAGTTCTCAGAGAGGACGATTGGAGCGTGGCGGCGGCGGAAATGTTCGGAGATGAGATGGAGTTGATGGATAACTACTACCGGCCTCCGAGGATATTTCTGGAGGAAACGAGCTTCCCGGAGACGGCGATGTTTTGCGGCGGTTTGTGCGACGGTGCGGTGGATTTGGACGGAAATTTCGGGTCATGTAAATGGGATGTGGGGACCTGTTTCGAATAA

mRNA sequence

ATGTCTGCAACTCACTCTCGCTTCAAGTTCCGTGAACGCCGCTCCGTCACCGGAATGCCTCTCCCGGCCGATTCCCCCGCCCCTAGGATCGTCCGCGTCTTCGTCACTGACTCTGACGCCACCGATACTTCCAGCAGCGACGACGACGACGAATTCGTCTGTCTCGTCTGCCGCCACATCAACGAGATACGCCTCCACGATTCTACGCACTCAAAGTCGAAGGAAGTCGACCCGATCAGAAAATTTCGCGGCGTGCGGCGGCGGCCGTGGGGGAAATTCGCGGCGGAGATTCGAGATCCGATTAAACGCACTAGAGTCTGGCTTGGAACCTTTGATTCGCCCGAGGAAGCCGCAATCGTCTACGATCAAGCAGCGATTCGATTCCGAGGTCCTGATGCCTTAACGAACATCGTCAAGCCGCCGCAACGGAATCCGCCGCCGGAGGAGATTTCTGATTCTTCTATGGAAATATCCGACGACCTCGCCGCCGTCTCCGGCGGAGGAATGCGTTCTTCTCCAACTTCAGTTCTCAGAGAGGACGATTGGAGCGTGGCGGCGGCGGAAATGTTCGGAGATGAGATGGAGTTGATGGATAACTACTACCGGCCTCCGAGGATATTTCTGGAGGAAACGAGCTTCCCGGAGACGGCGATGTTTTGCGGCGGTTTGTGCGACGGTGCGGTGGATTTGGACGGAAATTTCGGGTCATGTAAATGGGATGTGGGGACCTGTTTCGAATAA

Coding sequence (CDS)

ATGTCTGCAACTCACTCTCGCTTCAAGTTCCGTGAACGCCGCTCCGTCACCGGAATGCCTCTCCCGGCCGATTCCCCCGCCCCTAGGATCGTCCGCGTCTTCGTCACTGACTCTGACGCCACCGATACTTCCAGCAGCGACGACGACGACGAATTCGTCTGTCTCGTCTGCCGCCACATCAACGAGATACGCCTCCACGATTCTACGCACTCAAAGTCGAAGGAAGTCGACCCGATCAGAAAATTTCGCGGCGTGCGGCGGCGGCCGTGGGGGAAATTCGCGGCGGAGATTCGAGATCCGATTAAACGCACTAGAGTCTGGCTTGGAACCTTTGATTCGCCCGAGGAAGCCGCAATCGTCTACGATCAAGCAGCGATTCGATTCCGAGGTCCTGATGCCTTAACGAACATCGTCAAGCCGCCGCAACGGAATCCGCCGCCGGAGGAGATTTCTGATTCTTCTATGGAAATATCCGACGACCTCGCCGCCGTCTCCGGCGGAGGAATGCGTTCTTCTCCAACTTCAGTTCTCAGAGAGGACGATTGGAGCGTGGCGGCGGCGGAAATGTTCGGAGATGAGATGGAGTTGATGGATAACTACTACCGGCCTCCGAGGATATTTCTGGAGGAAACGAGCTTCCCGGAGACGGCGATGTTTTGCGGCGGTTTGTGCGACGGTGCGGTGGATTTGGACGGAAATTTCGGGTCATGTAAATGGGATGTGGGGACCTGTTTCGAATAA

Protein sequence

MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCLVCRHINEIRLHDSTHSKSKEVDPIRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGMRSSPTSVLREDDWSVAAAEMFGDEMELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGSCKWDVGTCFE
Homology
BLAST of Lsi08G003040 vs. ExPASy Swiss-Prot
Match: Q9M374 (Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN=CRF6 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.7e-31
Identity = 86/195 (44.10%), Postives = 111/195 (56.92%), Query Frame = 0

Query: 7   RFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCL---VCRHINEI 66
           R KF E R+VT +     + +PR+VR+ VTD  ATD+SS DDD+  V +   V R++ EI
Sbjct: 7   RVKFTENRTVTNVAATPSNGSPRLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKEI 66

Query: 67  RLHDSTHS--------KSKEVDPI------------RKFRGVRRRPWGKFAAEIRDPIKR 126
           R      S        K KE + +            +K+RGVR+RPWGKFAAEIRDP  R
Sbjct: 67  RFCQGESSSSTAARKGKHKEEESVVVEDDVSTSVKPKKYRGVRQRPWGKFAAEIRDPSSR 126

Query: 127 TRVWLGTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAA 179
           TR+WLGTF + EEAAI YD+AAI  +GP ALTN + PP   P  +  + S+ +   D   
Sbjct: 127 TRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTVSACDYGRD--- 186

BLAST of Lsi08G003040 vs. ExPASy Swiss-Prot
Match: O04682 (Pathogenesis-related genes transcriptional activator PTI6 OS=Solanum lycopersicum OX=4081 GN=PTI6 PE=2 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 8.5e-30
Identity = 78/178 (43.82%), Postives = 115/178 (64.61%), Query Frame = 0

Query: 23  ADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCLVCRHINEIRLHDSTHS----KSKEVDP 82
           ++S   R+VR+ +TD+DATD SS D+    V  V RH+ EI L  ST S    K + V P
Sbjct: 30  SNSELQRVVRIILTDADATD-SSDDEGRNTVRRVKRHVTEINLMPSTKSIGDRKRRSVSP 89

Query: 83  ------IRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYDQAAIRFRGPD 142
                  +KFRGVR+RPWG++AAEIRDP +  RVWLGT+D+PEEAA+VYD+AA++ +GPD
Sbjct: 90  DSDVTRRKKFRGVRQRPWGRWAAEIRDPTRGKRVWLGTYDTPEEAAVVYDKAAVKLKGPD 149

Query: 143 ALTNIVKPPQRNPPPEEISDSSMEISD-DLAAVSGGGMRS------SPTSVLREDDWS 184
           A+TN         P    ++ ++ +++ +  +V+ GG +S      SPTSVL ++D++
Sbjct: 150 AVTNF--------PVSTTAEVTVTVTETETESVADGGDKSENDVALSPTSVLCDNDFA 198

BLAST of Lsi08G003040 vs. ExPASy Swiss-Prot
Match: Q9SUE3 (Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN=CRF4 PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 8.0e-28
Identity = 81/176 (46.02%), Postives = 105/176 (59.66%), Query Frame = 0

Query: 29  RIVRVFVTDSDATDTSSSDDDDEFVC---LVCRHINEIRLHDSTHS------------KS 88
           R+VRV +TD DATD+SS  D++EF+     V R INEIR+  S+ S            K 
Sbjct: 41  RLVRVSMTDRDATDSSS--DEEEFLFPRRRVKRLINEIRVEPSSSSTGDVSASPTKDRKR 100

Query: 89  KEVDPI-----------RKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYD 148
             VD             +K+RGVR+RPWGK+AAEIRDP +R R+WLGTF + EEAAIVYD
Sbjct: 101 INVDSTVQKPSVSGQNQKKYRGVRQRPWGKWAAEIRDPEQRRRIWLGTFATAEEAAIVYD 160

Query: 149 QAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLA-AVSGGGMRSSPTSVL 178
            AAI+ RGPDALTN    P+  P  E+  +    +S  ++ ++      SSPTSVL
Sbjct: 161 NAAIKLRGPDALTNFTVQPEPEPVQEQEQEPESNMSVSISESMDDSQHLSSPTSVL 214

BLAST of Lsi08G003040 vs. ExPASy Swiss-Prot
Match: Q9SUQ2 (Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN=CRF2 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-27
Identity = 84/222 (37.84%), Postives = 117/222 (52.70%), Query Frame = 0

Query: 7   RFKFRERRSVTGMPLP--ADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCLVCRH----- 66
           R KF E ++ T   +    ++   RI+R+ VTD DATD+SS D+++E    V +      
Sbjct: 11  RIKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKK 70

Query: 67  -INEIRLHDST---------HSKSKE---------VDPI----------RKFRGVRRRPW 126
            +NE+ L              SK ++         V P+          +KFRGVR+RPW
Sbjct: 71  FVNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPW 130

Query: 127 GKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPP-----QRNP 179
           GK+AAEIRDP+KR R+WLGT+++ EEAA+VYD AAI+ RGPDALTN    P     ++ P
Sbjct: 131 GKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAP 190

BLAST of Lsi08G003040 vs. ExPASy Swiss-Prot
Match: O82503 (Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN=CRF1 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 1.5e-26
Identity = 77/174 (44.25%), Postives = 102/174 (58.62%), Query Frame = 0

Query: 28  PRIVRVFVTDSDATDTSSSDDDD-EFVCL------VCRHINEIRLHDSTHSKSKEVD--- 87
           PRI+R+ VTD  ATD+SS ++++ +F  L      V +++ E+ L      K K +    
Sbjct: 10  PRILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKR 69

Query: 88  -------PI------RKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYDQA 147
                  P+      RKFRGVR+RPWGK+AAEIRDP +R RVWLGTFD+ EEAAIVYD A
Sbjct: 70  KKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNA 129

Query: 148 AIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGG-MRSSPTSVL 178
           AI+ RGP+A  N   P    P  E + ++S E+      + GGG    SP SVL
Sbjct: 130 AIQLRGPNAELNFPPP----PVTENVEEASTEVKGVSDFIIGGGECLRSPVSVL 179

BLAST of Lsi08G003040 vs. ExPASy TrEMBL
Match: A0A5A7SSM5 (Ethylene-responsive transcription factor CRF6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold269G00880 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.8e-108
Identity = 199/249 (79.92%), Postives = 215/249 (86.35%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDD---EFVCLVC 60
           MS+THSRFKFRERRSVTG+ LP DS APRI+R+FVTDSDATDTSSSDDDD   + + LV 
Sbjct: 1   MSSTHSRFKFRERRSVTGITLPPDSAAPRILRIFVTDSDATDTSSSDDDDDDAQLLRLVR 60

Query: 61  RHINEIRLHDSTHSKSKEVDPIRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEA 120
           RHINEI  HDSTHSK  E+ P +KFRGVRRRPWGKFAAEIRDPIKRTR+WLGTFDSPEEA
Sbjct: 61  RHINEIHFHDSTHSKPNELQPTKKFRGVRRRPWGKFAAEIRDPIKRTRIWLGTFDSPEEA 120

Query: 121 AIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGMRSSPTSVL 180
           AIVYDQAAIRFRGPDALTNI+KPPQRN PPEEI DSSMEI++D     GGGMR SPTSVL
Sbjct: 121 AIVYDQAAIRFRGPDALTNIIKPPQRNLPPEEICDSSMEIAED-----GGGMRCSPTSVL 180

Query: 181 REDDWSVAAAEMFGDEMELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGSC 240
           R+D WS  A E+ GDEME MDNYY  PRIF+EE S PETAMFCGGL +GAVDLDGNFGSC
Sbjct: 181 RDDYWS--ATELLGDEMEFMDNYYGAPRIFVEERSIPETAMFCGGLWEGAVDLDGNFGSC 240

Query: 241 KWDVGTCFE 247
           KWDVGTCFE
Sbjct: 241 KWDVGTCFE 242

BLAST of Lsi08G003040 vs. ExPASy TrEMBL
Match: A0A1S3BE80 (ethylene-responsive transcription factor CRF6-like OS=Cucumis melo OX=3656 GN=LOC103488716 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.8e-108
Identity = 199/249 (79.92%), Postives = 215/249 (86.35%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDD---EFVCLVC 60
           MS+THSRFKFRERRSVTG+ LP DS APRI+R+FVTDSDATDTSSSDDDD   + + LV 
Sbjct: 1   MSSTHSRFKFRERRSVTGITLPPDSAAPRILRIFVTDSDATDTSSSDDDDDDAQLLRLVR 60

Query: 61  RHINEIRLHDSTHSKSKEVDPIRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEA 120
           RHINEI  HDSTHSK  E+ P +KFRGVRRRPWGKFAAEIRDPIKRTR+WLGTFDSPEEA
Sbjct: 61  RHINEIHFHDSTHSKPNELQPTKKFRGVRRRPWGKFAAEIRDPIKRTRIWLGTFDSPEEA 120

Query: 121 AIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGMRSSPTSVL 180
           AIVYDQAAIRFRGPDALTNI+KPPQRN PPEEI DSSMEI++D     GGGMR SPTSVL
Sbjct: 121 AIVYDQAAIRFRGPDALTNIIKPPQRNLPPEEICDSSMEIAED-----GGGMRCSPTSVL 180

Query: 181 REDDWSVAAAEMFGDEMELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGSC 240
           R+D WS  A E+ GDEME MDNYY  PRIF+EE S PETAMFCGGL +GAVDLDGNFGSC
Sbjct: 181 RDDYWS--ATELLGDEMEFMDNYYGAPRIFVEERSIPETAMFCGGLWEGAVDLDGNFGSC 240

Query: 241 KWDVGTCFE 247
           KWDVGTCFE
Sbjct: 241 KWDVGTCFE 242

BLAST of Lsi08G003040 vs. ExPASy TrEMBL
Match: A0A0A0KE47 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G042450 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 9.9e-106
Identity = 196/250 (78.40%), Postives = 212/250 (84.80%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDD----EFVCLV 60
           MS+ HSRFKFRERRSVTG+ LP D  APRI+R+FVTDSDATDTSSSDDDD      + LV
Sbjct: 1   MSSAHSRFKFRERRSVTGITLPPDYAAPRILRIFVTDSDATDTSSSDDDDNDDAHLLRLV 60

Query: 61  CRHINEIRLHDSTHSKSKEVDPIRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEE 120
            RHINEIR HDSTHSK  E+ P +KFRGVRRRPWGKFAAEIRDPIKRTR+WLGTFDSPEE
Sbjct: 61  RRHINEIRFHDSTHSKPNEIPPTKKFRGVRRRPWGKFAAEIRDPIKRTRIWLGTFDSPEE 120

Query: 121 AAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGMRSSPTSV 180
           AAIVYDQAAIRFRGPDALTNIVKPP RN P EEI DSSMEI++D     GGGMR SPTSV
Sbjct: 121 AAIVYDQAAIRFRGPDALTNIVKPPPRNVPAEEICDSSMEITED-----GGGMRCSPTSV 180

Query: 181 LREDDWSVAAAEMFGDEMELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGS 240
           LR++ W   A E+ GDEMELMDNYYR PRIF+EE S PETAMFCGGL +G+VDLDGNFGS
Sbjct: 181 LRDEYW--RATEVLGDEMELMDNYYRAPRIFVEERSLPETAMFCGGLWEGSVDLDGNFGS 240

Query: 241 CKWDVGTCFE 247
           CKWDVGTCFE
Sbjct: 241 CKWDVGTCFE 243

BLAST of Lsi08G003040 vs. ExPASy TrEMBL
Match: A0A6J1DA05 (ethylene-responsive transcription factor CRF2-like OS=Momordica charantia OX=3673 GN=LOC111018582 PE=4 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 5.0e-65
Identity = 150/262 (57.25%), Postives = 175/262 (66.79%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCLVCRHI 60
           M++ +SR +FRERRSVTG  L    P  RIVR+ VTD+DATDTSS  ++ EFV  V R +
Sbjct: 1   MTSIYSRVRFRERRSVTG-KLLRQLPGTRIVRISVTDADATDTSSG-EESEFVRRVSRRV 60

Query: 61  NEIRLHDSTHSKSKEVDPIR-----------KFRGVRRRPWGKFAAEIRDPIKRTRVWLG 120
           NEIR  D +  K +     R           KFR VRRR WGK+AAEIRDPIKR RVWLG
Sbjct: 61  NEIRFDDFSSPKQRTAKQGRDFPAKGESGGMKFRCVRRRQWGKWAAEIRDPIKRARVWLG 120

Query: 121 TFDSPEEAAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGM 180
           TFDSPEEAA+VYD+AAI+FRGPDALTNIVKPP+RNPPP E   +S +   D         
Sbjct: 121 TFDSPEEAAMVYDRAAIQFRGPDALTNIVKPPERNPPPPE---NSSDCGYD--------- 180

Query: 181 RSSPTSVLRE-DDWSVAAAEMFGDEMELMDNYY----RPPRIFLEETSFPETAMFCGGLC 240
             SPTSVLR   +WS AA E  GDE E +D  +    RP  IFLEETS PETA++ GG C
Sbjct: 181 --SPTSVLRSPPEWS-AAEEFVGDEFEFLDQRFVECHRPAPIFLEETSVPETAIWGGGFC 240

Query: 241 DGAVDLDGNFGSCKWDVGTCFE 247
           DG  DLDGNFGS KWDV + F+
Sbjct: 241 DGPADLDGNFGSWKWDVDSYFQ 245

BLAST of Lsi08G003040 vs. ExPASy TrEMBL
Match: A0A2P5YTL8 (AP2/ERF domain-containing protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA01665 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 5.9e-42
Identity = 120/287 (41.81%), Postives = 155/287 (54.01%), Query Frame = 0

Query: 7   RFKFRERRSVTGMPLPADS---------PAPRIVRVFVTDSDATDTSSSDDDDEFVCLVC 66
           R K+ ERR+VT M L A S          APRIVR+ VTD DATD+SS + + E    V 
Sbjct: 5   RVKYTERRTVTNMLLKASSKWFPMESKPKAPRIVRISVTDGDATDSSSDESEQERHHTVK 64

Query: 67  RHINEIRLHD-----STHSKSKEVDPIRK----FRGVRRRPWGKFAAEIRDPIKRTRVWL 126
           RH++EIR+ D     S  + +K+  P       +RGVR+RPWG++AAEIRDP  RTRVWL
Sbjct: 65  RHVSEIRIGDRSTFNSNKTSNKQNRPTNNKTNLYRGVRQRPWGRWAAEIRDPTSRTRVWL 124

Query: 127 GTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGG 186
           GT+D+ EEAA+VYD+AAIR +GPDALTN +KPP R  PPE      +E++    +     
Sbjct: 125 GTYDTAEEAALVYDRAAIRIKGPDALTNFIKPPARPSPPE----IELEMTSGYDSGQESH 184

Query: 187 MRSSPTSVLR-------------EDD------WSVAAAEMFGDEMELMDNYY-------- 242
              SPTSVLR             +DD      WS    E+      L+D +         
Sbjct: 185 SLCSPTSVLRFQSNEETELQTESKDDSTTQTGWS-PVKELSQYPSNLLDEFLLTDPVVLY 244

BLAST of Lsi08G003040 vs. NCBI nr
Match: XP_038886548.1 (ethylene-responsive transcription factor CRF6-like [Benincasa hispida])

HSP 1 Score: 434.5 bits (1116), Expect = 6.1e-118
Identity = 212/249 (85.14%), Postives = 226/249 (90.76%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDD---EFVCLVC 60
           MS+T SRFKFRERRSVTGM LPADS APRIVR+ VTDSDATDTSSSDDDD   + V LV 
Sbjct: 1   MSSTQSRFKFRERRSVTGMSLPADSTAPRIVRILVTDSDATDTSSSDDDDNDAQLVRLVS 60

Query: 61  RHINEIRLHDSTHSKSKEVDPIRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEA 120
           RHINEI LH+STHSK KEV PI+KFRGVRRRPWGKFAAEIRDP KRTR+WLGT+DSPEEA
Sbjct: 61  RHINEIHLHNSTHSKLKEVKPIKKFRGVRRRPWGKFAAEIRDPNKRTRIWLGTYDSPEEA 120

Query: 121 AIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGMRSSPTSVL 180
           AIVYDQAAIRFRGPDALTNI+KPPQRNPPPEEI DSSM+I+DDL+AVSGGGM  SPTS+L
Sbjct: 121 AIVYDQAAIRFRGPDALTNILKPPQRNPPPEEIYDSSMQITDDLSAVSGGGMWFSPTSIL 180

Query: 181 REDDWSVAAAEMFGDEMELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGSC 240
           R+DDW+ AAAEM GDEMELMD YYRPPRIFLEETSFPE AMFCGG CDGAVDLDGNFGSC
Sbjct: 181 RDDDWNAAAAEMLGDEMELMDYYYRPPRIFLEETSFPEMAMFCGGFCDGAVDLDGNFGSC 240

Query: 241 KWDVGTCFE 247
           KWDVGT FE
Sbjct: 241 KWDVGTYFE 249

BLAST of Lsi08G003040 vs. NCBI nr
Match: XP_008445792.1 (PREDICTED: ethylene-responsive transcription factor CRF6-like [Cucumis melo] >KAA0032996.1 ethylene-responsive transcription factor CRF6-like [Cucumis melo var. makuwa])

HSP 1 Score: 401.4 bits (1030), Expect = 5.8e-108
Identity = 199/249 (79.92%), Postives = 215/249 (86.35%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDD---EFVCLVC 60
           MS+THSRFKFRERRSVTG+ LP DS APRI+R+FVTDSDATDTSSSDDDD   + + LV 
Sbjct: 1   MSSTHSRFKFRERRSVTGITLPPDSAAPRILRIFVTDSDATDTSSSDDDDDDAQLLRLVR 60

Query: 61  RHINEIRLHDSTHSKSKEVDPIRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEA 120
           RHINEI  HDSTHSK  E+ P +KFRGVRRRPWGKFAAEIRDPIKRTR+WLGTFDSPEEA
Sbjct: 61  RHINEIHFHDSTHSKPNELQPTKKFRGVRRRPWGKFAAEIRDPIKRTRIWLGTFDSPEEA 120

Query: 121 AIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGMRSSPTSVL 180
           AIVYDQAAIRFRGPDALTNI+KPPQRN PPEEI DSSMEI++D     GGGMR SPTSVL
Sbjct: 121 AIVYDQAAIRFRGPDALTNIIKPPQRNLPPEEICDSSMEIAED-----GGGMRCSPTSVL 180

Query: 181 REDDWSVAAAEMFGDEMELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGSC 240
           R+D WS  A E+ GDEME MDNYY  PRIF+EE S PETAMFCGGL +GAVDLDGNFGSC
Sbjct: 181 RDDYWS--ATELLGDEMEFMDNYYGAPRIFVEERSIPETAMFCGGLWEGAVDLDGNFGSC 240

Query: 241 KWDVGTCFE 247
           KWDVGTCFE
Sbjct: 241 KWDVGTCFE 242

BLAST of Lsi08G003040 vs. NCBI nr
Match: XP_011656563.1 (ethylene-responsive transcription factor CRF6 [Cucumis sativus] >KGN46011.1 hypothetical protein Csa_005043 [Cucumis sativus])

HSP 1 Score: 392.9 bits (1008), Expect = 2.0e-105
Identity = 196/250 (78.40%), Postives = 212/250 (84.80%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDD----EFVCLV 60
           MS+ HSRFKFRERRSVTG+ LP D  APRI+R+FVTDSDATDTSSSDDDD      + LV
Sbjct: 1   MSSAHSRFKFRERRSVTGITLPPDYAAPRILRIFVTDSDATDTSSSDDDDNDDAHLLRLV 60

Query: 61  CRHINEIRLHDSTHSKSKEVDPIRKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEE 120
            RHINEIR HDSTHSK  E+ P +KFRGVRRRPWGKFAAEIRDPIKRTR+WLGTFDSPEE
Sbjct: 61  RRHINEIRFHDSTHSKPNEIPPTKKFRGVRRRPWGKFAAEIRDPIKRTRIWLGTFDSPEE 120

Query: 121 AAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGMRSSPTSV 180
           AAIVYDQAAIRFRGPDALTNIVKPP RN P EEI DSSMEI++D     GGGMR SPTSV
Sbjct: 121 AAIVYDQAAIRFRGPDALTNIVKPPPRNVPAEEICDSSMEITED-----GGGMRCSPTSV 180

Query: 181 LREDDWSVAAAEMFGDEMELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGS 240
           LR++ W   A E+ GDEMELMDNYYR PRIF+EE S PETAMFCGGL +G+VDLDGNFGS
Sbjct: 181 LRDEYW--RATEVLGDEMELMDNYYRAPRIFVEERSLPETAMFCGGLWEGSVDLDGNFGS 240

Query: 241 CKWDVGTCFE 247
           CKWDVGTCFE
Sbjct: 241 CKWDVGTCFE 243

BLAST of Lsi08G003040 vs. NCBI nr
Match: XP_022150419.1 (ethylene-responsive transcription factor CRF2-like [Momordica charantia])

HSP 1 Score: 257.7 bits (657), Expect = 1.0e-64
Identity = 150/262 (57.25%), Postives = 175/262 (66.79%), Query Frame = 0

Query: 1   MSATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCLVCRHI 60
           M++ +SR +FRERRSVTG  L    P  RIVR+ VTD+DATDTSS  ++ EFV  V R +
Sbjct: 1   MTSIYSRVRFRERRSVTG-KLLRQLPGTRIVRISVTDADATDTSSG-EESEFVRRVSRRV 60

Query: 61  NEIRLHDSTHSKSKEVDPIR-----------KFRGVRRRPWGKFAAEIRDPIKRTRVWLG 120
           NEIR  D +  K +     R           KFR VRRR WGK+AAEIRDPIKR RVWLG
Sbjct: 61  NEIRFDDFSSPKQRTAKQGRDFPAKGESGGMKFRCVRRRQWGKWAAEIRDPIKRARVWLG 120

Query: 121 TFDSPEEAAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGGM 180
           TFDSPEEAA+VYD+AAI+FRGPDALTNIVKPP+RNPPP E   +S +   D         
Sbjct: 121 TFDSPEEAAMVYDRAAIQFRGPDALTNIVKPPERNPPPPE---NSSDCGYD--------- 180

Query: 181 RSSPTSVLRE-DDWSVAAAEMFGDEMELMDNYY----RPPRIFLEETSFPETAMFCGGLC 240
             SPTSVLR   +WS AA E  GDE E +D  +    RP  IFLEETS PETA++ GG C
Sbjct: 181 --SPTSVLRSPPEWS-AAEEFVGDEFEFLDQRFVECHRPAPIFLEETSVPETAIWGGGFC 240

Query: 241 DGAVDLDGNFGSCKWDVGTCFE 247
           DG  DLDGNFGS KWDV + F+
Sbjct: 241 DGPADLDGNFGSWKWDVDSYFQ 245

BLAST of Lsi08G003040 vs. NCBI nr
Match: XP_031278720.1 (ethylene-responsive transcription factor CRF6 [Pistacia vera])

HSP 1 Score: 182.2 bits (461), Expect = 5.5e-42
Identity = 118/292 (40.41%), Postives = 161/292 (55.14%), Query Frame = 0

Query: 3   ATHSRFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDD-EFVCLVCRHIN 62
           A  SR K+ E R++T   +       ++VR+ VTD DATD+SS +D+D      V +HIN
Sbjct: 4   AISSRTKYSEHRTITTKLVKPSRFQTKVVRILVTDVDATDSSSDEDEDYSSPARVKKHIN 63

Query: 63  EIRLHD----------STHSKSKEVDPIR----------KFRGVRRRPWGKFAAEIRDPI 122
           EI++ D          + +++ +   PI+          K+RGVR+RPWG++AAEIRDP 
Sbjct: 64  EIKIEDCSIFTSENVVNNNNRDRITKPIQQKASGRSNGNKYRGVRQRPWGRWAAEIRDPS 123

Query: 123 KRTRVWLGTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDL 182
           +RTRVWLGTFD+ EEAA+VYD+AAIR +GPDA TN +KPP+RN       D++  +  D+
Sbjct: 124 RRTRVWLGTFDTAEEAALVYDKAAIRIKGPDAQTNFMKPPERN-------DNTTPLEIDV 183

Query: 183 AAVSG--GGMRS----SPTSVLR---------------------EDDWSVAAAEMFGDEM 242
             VSG   G  S    SPTSVLR                     + +W      +F DE 
Sbjct: 184 VTVSGYDSGKESQTIYSPTSVLRFQSNENEEAEQRTEYQPETETDSEWRKVQDCLFLDEG 243

Query: 243 ELMDNYYRPPRIFLEETSFPETAMFCGGLCDGAVDLDGNFGSCKWDVGTCFE 247
            L  +   PPRIF +E S P   +    L D +V LDG+FGSC WDV   F+
Sbjct: 244 FL--DSETPPRIFFDEMSVP---ILAENLDDISVSLDGDFGSCIWDVDNYFQ 283

BLAST of Lsi08G003040 vs. TAIR 10
Match: AT3G61630.1 (cytokinin response factor 6 )

HSP 1 Score: 137.1 bits (344), Expect = 1.9e-32
Identity = 86/195 (44.10%), Postives = 111/195 (56.92%), Query Frame = 0

Query: 7   RFKFRERRSVTGMPLPADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCL---VCRHINEI 66
           R KF E R+VT +     + +PR+VR+ VTD  ATD+SS DDD+  V +   V R++ EI
Sbjct: 7   RVKFTENRTVTNVAATPSNGSPRLVRITVTDPFATDSSSDDDDNNNVTVVPRVKRYVKEI 66

Query: 67  RLHDSTHS--------KSKEVDPI------------RKFRGVRRRPWGKFAAEIRDPIKR 126
           R      S        K KE + +            +K+RGVR+RPWGKFAAEIRDP  R
Sbjct: 67  RFCQGESSSSTAARKGKHKEEESVVVEDDVSTSVKPKKYRGVRQRPWGKFAAEIRDPSSR 126

Query: 127 TRVWLGTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAA 179
           TR+WLGTF + EEAAI YD+AAI  +GP ALTN + PP   P  +  + S+ +   D   
Sbjct: 127 TRIWLGTFVTAEEAAIAYDRAAIHLKGPKALTNFLTPPTPTPVIDLQTVSACDYGRD--- 186

BLAST of Lsi08G003040 vs. TAIR 10
Match: AT4G27950.1 (cytokinin response factor 4 )

HSP 1 Score: 125.6 bits (314), Expect = 5.7e-29
Identity = 81/176 (46.02%), Postives = 105/176 (59.66%), Query Frame = 0

Query: 29  RIVRVFVTDSDATDTSSSDDDDEFVC---LVCRHINEIRLHDSTHS------------KS 88
           R+VRV +TD DATD+SS  D++EF+     V R INEIR+  S+ S            K 
Sbjct: 41  RLVRVSMTDRDATDSSS--DEEEFLFPRRRVKRLINEIRVEPSSSSTGDVSASPTKDRKR 100

Query: 89  KEVDPI-----------RKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYD 148
             VD             +K+RGVR+RPWGK+AAEIRDP +R R+WLGTF + EEAAIVYD
Sbjct: 101 INVDSTVQKPSVSGQNQKKYRGVRQRPWGKWAAEIRDPEQRRRIWLGTFATAEEAAIVYD 160

Query: 149 QAAIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLA-AVSGGGMRSSPTSVL 178
            AAI+ RGPDALTN    P+  P  E+  +    +S  ++ ++      SSPTSVL
Sbjct: 161 NAAIKLRGPDALTNFTVQPEPEPVQEQEQEPESNMSVSISESMDDSQHLSSPTSVL 214

BLAST of Lsi08G003040 vs. TAIR 10
Match: AT4G23750.1 (cytokinin response factor 2 )

HSP 1 Score: 124.8 bits (312), Expect = 9.7e-29
Identity = 84/222 (37.84%), Postives = 117/222 (52.70%), Query Frame = 0

Query: 7   RFKFRERRSVTGMPLP--ADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCLVCRH----- 66
           R KF E ++ T   +    ++   RI+R+ VTD DATD+SS D+++E    V +      
Sbjct: 11  RIKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKK 70

Query: 67  -INEIRLHDST---------HSKSKE---------VDPI----------RKFRGVRRRPW 126
            +NE+ L              SK ++         V P+          +KFRGVR+RPW
Sbjct: 71  FVNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPW 130

Query: 127 GKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPP-----QRNP 179
           GK+AAEIRDP+KR R+WLGT+++ EEAA+VYD AAI+ RGPDALTN    P     ++ P
Sbjct: 131 GKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAP 190

BLAST of Lsi08G003040 vs. TAIR 10
Match: AT4G23750.2 (cytokinin response factor 2 )

HSP 1 Score: 124.8 bits (312), Expect = 9.7e-29
Identity = 84/222 (37.84%), Postives = 117/222 (52.70%), Query Frame = 0

Query: 7   RFKFRERRSVTGMPLP--ADSPAPRIVRVFVTDSDATDTSSSDDDDEFVCLVCRH----- 66
           R KF E ++ T   +    ++   RI+R+ VTD DATD+SS D+++E    V +      
Sbjct: 11  RIKFTEHKTNTTTIVSELTNTHQTRILRISVTDPDATDSSSDDEEEEHQRFVSKRRRVKK 70

Query: 67  -INEIRLHDST---------HSKSKE---------VDPI----------RKFRGVRRRPW 126
            +NE+ L              SK ++         V P+          +KFRGVR+RPW
Sbjct: 71  FVNEVYLDSGAVVTGSCGQMESKKRQKRAVKSESTVSPVVSATTTTTGEKKFRGVRQRPW 130

Query: 127 GKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYDQAAIRFRGPDALTNIVKPP-----QRNP 179
           GK+AAEIRDP+KR R+WLGT+++ EEAA+VYD AAI+ RGPDALTN    P     ++ P
Sbjct: 131 GKWAAEIRDPLKRVRLWLGTYNTAEEAAMVYDNAAIQLRGPDALTNFSVTPTTATEKKAP 190

BLAST of Lsi08G003040 vs. TAIR 10
Match: AT4G11140.1 (cytokinin response factor 1 )

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-27
Identity = 77/174 (44.25%), Postives = 102/174 (58.62%), Query Frame = 0

Query: 28  PRIVRVFVTDSDATDTSSSDDDD-EFVCL------VCRHINEIRLHDSTHSKSKEVD--- 87
           PRI+R+ VTD  ATD+SS ++++ +F  L      V +++ E+ L      K K +    
Sbjct: 10  PRILRISVTDPYATDSSSDEEEEVDFDALSTKRRRVKKYVKEVVLDSVVSDKEKPMKKKR 69

Query: 88  -------PI------RKFRGVRRRPWGKFAAEIRDPIKRTRVWLGTFDSPEEAAIVYDQA 147
                  P+      RKFRGVR+RPWGK+AAEIRDP +R RVWLGTFD+ EEAAIVYD A
Sbjct: 70  KKRVVTVPVVVTTATRKFRGVRQRPWGKWAAEIRDPSRRVRVWLGTFDTAEEAAIVYDNA 129

Query: 148 AIRFRGPDALTNIVKPPQRNPPPEEISDSSMEISDDLAAVSGGG-MRSSPTSVL 178
           AI+ RGP+A  N   P    P  E + ++S E+      + GGG    SP SVL
Sbjct: 130 AIQLRGPNAELNFPPP----PVTENVEEASTEVKGVSDFIIGGGECLRSPVSVL 179

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3742.7e-3144.10Ethylene-responsive transcription factor CRF6 OS=Arabidopsis thaliana OX=3702 GN... [more]
O046828.5e-3043.82Pathogenesis-related genes transcriptional activator PTI6 OS=Solanum lycopersicu... [more]
Q9SUE38.0e-2846.02Ethylene-responsive transcription factor CRF4 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9SUQ21.4e-2737.84Ethylene-responsive transcription factor CRF2 OS=Arabidopsis thaliana OX=3702 GN... [more]
O825031.5e-2644.25Ethylene-responsive transcription factor CRF1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A5A7SSM52.8e-10879.92Ethylene-responsive transcription factor CRF6-like OS=Cucumis melo var. makuwa O... [more]
A0A1S3BE802.8e-10879.92ethylene-responsive transcription factor CRF6-like OS=Cucumis melo OX=3656 GN=LO... [more]
A0A0A0KE479.9e-10678.40AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G042450 PE=... [more]
A0A6J1DA055.0e-6557.25ethylene-responsive transcription factor CRF2-like OS=Momordica charantia OX=367... [more]
A0A2P5YTL85.9e-4241.81AP2/ERF domain-containing protein OS=Gossypium barbadense OX=3634 GN=GOBAR_AA016... [more]
Match NameE-valueIdentityDescription
XP_038886548.16.1e-11885.14ethylene-responsive transcription factor CRF6-like [Benincasa hispida][more]
XP_008445792.15.8e-10879.92PREDICTED: ethylene-responsive transcription factor CRF6-like [Cucumis melo] >KA... [more]
XP_011656563.12.0e-10578.40ethylene-responsive transcription factor CRF6 [Cucumis sativus] >KGN46011.1 hypo... [more]
XP_022150419.11.0e-6457.25ethylene-responsive transcription factor CRF2-like [Momordica charantia][more]
XP_031278720.15.5e-4240.41ethylene-responsive transcription factor CRF6 [Pistacia vera][more]
Match NameE-valueIdentityDescription
AT3G61630.11.9e-3244.10cytokinin response factor 6 [more]
AT4G27950.15.7e-2946.02cytokinin response factor 4 [more]
AT4G23750.19.7e-2937.84cytokinin response factor 2 [more]
AT4G23750.29.7e-2937.84cytokinin response factor 2 [more]
AT4G11140.11.1e-2744.25cytokinin response factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 82..93
score: 66.97
coord: 104..120
score: 60.43
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 81..144
e-value: 1.4E-34
score: 130.9
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 81..130
e-value: 5.1E-13
score: 49.1
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 81..138
score: 21.95706
IPR001471AP2/ERF domainCDDcd00018AP2coord: 81..136
e-value: 3.81206E-30
score: 105.046
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 80..138
e-value: 1.3E-23
score: 84.9
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 207..246
NoneNo IPR availablePANTHERPTHR31194:SF108PATHOGENESIS-RELATED GENES TRANSCRIPTIONAL ACTIVATOR PTI6-LIKEcoord: 207..246
NoneNo IPR availablePANTHERPTHR31194:SF108PATHOGENESIS-RELATED GENES TRANSCRIPTIONAL ACTIVATOR PTI6-LIKEcoord: 6..189
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 6..189
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 81..137

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi08G003040.1Lsi08G003040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity