Lsi08G002820 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G002820
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial
Location	chr08: 7110301 .. 7121541 (+)
RNA-Seq Expression	Lsi08G002820
Synteny	Lsi08G002820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGAGTTGGATTTTCCAGGAGGAAGAGTTCCATTTACGAATGAAATGAGATTCATTGCTGAGTCAACTCAAAAGCGAGTTCCGTGTTATCGTGTACTTCATGAAAATGGGGACATTCTTACATCAAACAATTTCATACAGGTGTTACTCTTATATTATTATTATTATTATTATTATTATTAATTAATTAATTAAATATAAAATAAATAAATTTCATCTCTAATATCCCTAATAACGGTTTTTTATAACGCTTTATCAAGTAAACTAATTTGATGAAATTAAAAGTTGTGAAATTGATACATAATAAAAAAGAAGAAGTAAAAAAGATATTATCGTATTAACCCTATTATATAATTTGATTATCATAATATTTTAGTGTTTGTACTTGGAATTTGATAGTTGAGGAAAGATGTAGCGATTAAGATGTACAAAGACATGATTACCCTTCAAACAATGGACACCATATTGTACGAAGCACAAAGACAAGGAAGAATCTCATTTTACTTAACTTCATCGGGAGAAGAAGCCATTGCCATTGCTTCTGCAGCTGCACTTCTACCCGACGACGTCGTTTTGGCTCAGGTATATTATTATTATTATTATTGAATTATTGGTAATATTAATTTTAAAGTAAGGAAATTAGTAAAAAAAATGTGAAAGGATTGATTATATTTGGCAGTATAGAGAGCCGGGTGTGTTATTATGGCGTGGGTTTACCTTGCAAGAATTTGCAAATCAATGCTTTGGAAACAAAGACGATTATGGAAAGGGAAGACAAATGCCCATTCACTATGGCTCCAACCAACACAACTACCTCACAATTTCATCTCCCCTTGGGTAATTCCTTTCATCCCATCAACCTTCTCTCTATCACAAATCATTTCAATCAACCACACACGTTACATAGAGACGTCCACCGACTGGGACGGGACTGCTCCCCATTCCCCTTCCAAATGAGGAATTCGTGGAGATTGGATTCCCCACGCGAATCACTATCAATTAACATAAATCACTATTATGGGAATGATTTTAATTAAATGGTTAATTTTTTATATCATTAGTTTCACATAGACAATTAGAATTTAAAGAGAAATTGCTCAAAATTTTTAAGTTGAACAAAATAATTATTTATCAAATTATTTATATATATAATCTAAATTTATTAGAATTCAAATCATGTCTTTTTTCTTCCACTAATTCAAAATATTTATTATTTTGACTATTAAACAATAAATTTTGAGAATTAAATATAATATTTATATAATAAATATAAGAAAAAATCTAATGGGGTGGCAACTGAGAATTTTTTCCCCTTCCCCATTCTCCACTCCCATGGAAAAAGTGGACATCTCTAAGATTAGGGGGAAAATTTTCATATACTATAGTATTATGAAAAATTTTCCTTACCACTTTCACTTATTGTATGTCACGTTAACACAGTTTTTTAAAAATAGGATGAGATACACTAACATTAATGTGGGGTCCTAGATATTTATGAAGACAGATTTATAGTGTGAAAAGGAGAGTATGATACTCTCGTGGTACTAGAAAAATTCTCATAGATTAGATTAGGTAGTGCATACTACTTTCATTCAATATTTGACACATCATTCAATGATATATTATTTTAAATAACAAATTGTTATCAAACTTGATATTACTTTTCAAATACCTAATAAAAATAATAAAAAAATAAAAACGCAAAATTTTTACCAAGAAAATGTTAGTCTTCACATTTTAACCTTATTATTGAAAACCAAAGACATTAAACCAGAATTGTGTAATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACATTGTGGATAATTTTCAATATTGCATACAATTTCAAATCTAAGAAATTAAAAAAATGATTTAAATTTGTGTATTTGGTTAAAAATTGCTATTAATGTATTTGTCATTGAACTGAAACGATGTCGTTTTATGTTTCAGAACACAACTTCCACAAGCTGTTGGCATTGCTTATTCCCTTAAAATGGATAAAAAGGATGCATGTGCTGTGGCTTTTTTTGGAGATGGCACCACCAGCGAGGTAAATTAAACTCCAATTATTAATAATCTAGGTTAAGTATTTCGGGAATTTTAAAAAATAGAAAAATAAGATAAATTATTTACATAAAATAGCAAATTTTTTAAGATAGTTATGATAGACGCGGATAGATGTCTATCAGAATCTATCAGTGATAGAAATGACAGAAGTCATTCACTGATAGATGTTGATAGAAGTCTATCAATGTCTATCAGTATTTTTTTTGCTACTTTTTTGTAAATAGTTTGACATTTTTTTTTATTTGTGAAAATTTCTCTAAGTATTATGTTTAGTCTCTAATGTTTGAGTATTTTCTTTATTTAATTTAGTCTGTATGATTTTAAAAATTTTAGGTTTCGTTTTTTATGCTTCAACACGATTATATTATTATTTTTTTTTTGTTGGACAAGATTAAAATTTGTTAATACATAATGAATTTAACAAAATTGTAGAAAAAAAGAACTAGGGCTACTTAAAAGGGGAAAGTTCTGAGGAACAAATAGAAATTTTTATTTTTCCTCTTAAGTAGTTTCATTACTCTTTAGAAATTTTTGACAATATATCTGATGGAGGATTGAAGCTTAGACCTCGAGGTTGATAATAGAAGTTCATATCAACTGAGCTATGCTTTTGTTGGCAAGTATAATGTCATGTTAGTTTATATTCATTGATTAATTTTAACCGATACAGGTTAAAAGACAAAAGCATTCAAACATGAGATATAAAAAAATATATATATTTAATCTCTAATTTATACTTGTTTTACTTCTAATAATCTTTAATTTTTAGCTTTTACATTTATTAGTTATTGTAAGAAAAATCAATTTTACAATTGGAAAATATTATAACAAATTATTACAAAATTTTAAATTAAAGAATTAGATAAGAACATTGATGGTAGACTATCTTATCTCTTACTTAAAAAGAATAGAAAATAGAATTTTAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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ATGGACCAAATTCTAATTTTTAAAATCATAAAAACTAAATTCTAACTTTTTCCAAACTATAAGAACCAAATTTATAATTTAACTCAAAATATCACTGTCTAGGAGTGAAAGACACTATTGATGTCTATTATTGTCTATCAATGTCTATAAGTGAAATTTTGGTGTAGTTTAAAATAATAGTAATTTTTTGGTGTGGTTTCCATTCTCTAGATTTTAAATTTTGATTTTAATTTAGTCTTTAAGTTTCAAAATGTTACAATTTTACCCTTGAAAGTTTGAGTTTAAAGTCAACATACTTTTAATCATTCAAAATGATTTTGATGATATGAAAATTGTATTTAAAAGTGTAAAATTAATATTAGTTTAATTTTGAGTGACTAAAGACATGTTTAATGATTTTAAACTTGACAAAATGATTTTAACAATTTTAAAATCACTCCCTAAACATGTACTTAACCTCGATTTTCACTACATATTCATTTTCAATCTATAGCGTTGATGTTTATTAATTAATTGAAAAGAATTAAAAGCATCATAATTAATTAAGTTTCATTGTTTTTTTAATCATAATTGTTTAAAATTAATTAATAGACATTAATGCCAAAGTGAATATTTAGTGAAAAATCAAGGTTAAAAAGTGTGAATCTTGAAATCTAAAGACCAAATTGAAACAAAGATCAAATCTTAAGCATAAAATTGTAACATTTTGATACTTACAGATTAAATTGAAATCAAACTCAAAACTTAAGAACTTAAATGTATAATAATTTTAAACCTAAGGACCAAATAGAAACTAGACCCAAAACATTAATTAATTTATAATCAATAATGTATTTTAATAGTGCCCATTTTGTTGGATCAGTTGTTGCAAGCAATTCAGAATGCAGAGAAAACAGAGAAGCCTCCAGTTTCAGAGTTGTTTAATGATGTTTATGATCATTTACCTAAAAACCTTGAAGAACAAGAGGAAGTATTAAGGCAAACAATTAAGAGGTATCCTCAAGATTATCCTTCTGATGTTCCTCTTTAGATTATTCTATCAAAGACATTGTCAACTGTCCAAAATGGAAGCTTGAGTCAAATAAATGTAAACTCATATGATTTGATATCATGTAATATCAGCTTTGTGGGTTAATATCATGCTATACCTCTTTGGGAACTCTTGGCAATGATAAAGTTAGTGTAAATTGATATGAAACTCAATAACATTTTGTAATGAATAAAATCTTATTCTTACGGAG

mRNA sequence

AGGAGTTGGATTTTCCAGGAGGAAGAGTTCCATTTACGAATGAAATGAGATTCATTGCTGAGTCAACTCAAAAGCGAGTTCCGTGTTATCGTGTACTTCATGAAAATGGGGACATTCTTACATCAAACAATTTCATACAGTTGAGGAAAGATGTAGCGATTAAGATGTACAAAGACATGATTACCCTTCAAACAATGGACACCATATTGTACGAAGCACAAAGACAAGGAAGAATCTCATTTTACTTAACTTCATCGGGAGAAGAAGCCATTGCCATTGCTTCTGCAGCTGCACTTCTACCCGACGACGTCGTTTTGGCTCAGGGGGATTTCCACGCGGCATTGAACTTCGCAGCCGTATTAGGAGCGCCGGTGATATTCATCTGCCGGAACAACGGCTGGGCCATCAGCACTTCCGTCCAAGAACAATTCCGAAGCGACGGCGTGGTGGTAAAAGGTCAGGGCTATGGAATCCGAAGCATCCGAGTCGACGGAAACGATGCCCTAGCCGTTTACAGTGCGGTTCGCAGAGCTCGATCCATGGCGGTTAAAGAGCAAATGCCGATTCTCATCGAGGCTTTAACTTACAGAGTGGGCCACCATTCCACCTCCGATGATTCCAGTAAGTATCGCGCCGTTGATGAGATTCAGTACTGGAAAATGGAGAGGAATCCTGTGGATCGCTTTACTAAATGGCTTTTCAATAATGGATGGTTGAATCAAGAACACGACTCCAATCACAAATCTACCGTCAGAAAACAGTTGTTGCAAGCAATTCAGAATGCAGAGAAAACAGAGAAGCCTCCAGTTTCAGAGTTGTTTAATGATGTTTATGATCATTTACCTAAAAACCTTGAAGAACAAGAGGAAGTATTAAGGCAAACAATTAAGAGGTATCCTCAAGATTATCCTTCTGATGTTCCTCTTTAGATTATTCTATCAAAGACATTGTCAACTGTCCAAAATGGAAGCTTGAGTCAAATAAATGTAAACTCATATGATTTGATATCATGTAATATCAGCTTTGTGGGTTAATATCATGCTATACCTCTTTGGGAACTCTTGGCAATGATAAAGTTAGTGTAAATTGATATGAAACTCAATAACATTTTGTAATGAATAAAATCTTATTCTTACGGAG

Coding sequence (CDS)

ATGAGATTCATTGCTGAGTCAACTCAAAAGCGAGTTCCGTGTTATCGTGTACTTCATGAAAATGGGGACATTCTTACATCAAACAATTTCATACAGTTGAGGAAAGATGTAGCGATTAAGATGTACAAAGACATGATTACCCTTCAAACAATGGACACCATATTGTACGAAGCACAAAGACAAGGAAGAATCTCATTTTACTTAACTTCATCGGGAGAAGAAGCCATTGCCATTGCTTCTGCAGCTGCACTTCTACCCGACGACGTCGTTTTGGCTCAGGGGGATTTCCACGCGGCATTGAACTTCGCAGCCGTATTAGGAGCGCCGGTGATATTCATCTGCCGGAACAACGGCTGGGCCATCAGCACTTCCGTCCAAGAACAATTCCGAAGCGACGGCGTGGTGGTAAAAGGTCAGGGCTATGGAATCCGAAGCATCCGAGTCGACGGAAACGATGCCCTAGCCGTTTACAGTGCGGTTCGCAGAGCTCGATCCATGGCGGTTAAAGAGCAAATGCCGATTCTCATCGAGGCTTTAACTTACAGAGTGGGCCACCATTCCACCTCCGATGATTCCAGTAAGTATCGCGCCGTTGATGAGATTCAGTACTGGAAAATGGAGAGGAATCCTGTGGATCGCTTTACTAAATGGCTTTTCAATAATGGATGGTTGAATCAAGAACACGACTCCAATCACAAATCTACCGTCAGAAAACAGTTGTTGCAAGCAATTCAGAATGCAGAGAAAACAGAGAAGCCTCCAGTTTCAGAGTTGTTTAATGATGTTTATGATCATTTACCTAAAAACCTTGAAGAACAAGAGGAAGTATTAAGGCAAACAATTAAGAGGTATCCTCAAGATTATCCTTCTGATGTTCCTCTTTAG

Protein sequence

MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQRQGRISFYLTSSGEEAIAIASAAALLPDDVVLAQGDFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDALAVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRFTKWLFNNGWLNQEHDSNHKSTVRKQLLQAIQNAEKTEKPPVSELFNDVYDHLPKNLEEQEEVLRQTIKRYPQDYPSDVPL

Homology

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BLAST of Lsi08G002820 vs. ExPASy Swiss-Prot
Match: Q9LPL5 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g21400 PE=2 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 3.9e-106
Identity = 208/375 (55.47%), Postives = 242/375 (64.53%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           M+FI ES+ +R+PCYRVL E+G I+  ++FI + + +A++MY+ M TLQ MD I YEAQR
Sbjct: 93  MKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQR 152

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTS GEEAI IASAAAL PDDVVL Q                           
Sbjct: 153 QGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKAD 212

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 213 YGKGRQMPIHYGSNRLNYFTISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEG 272

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHA LNFAAV+ APV+FICRNNGWAIST + EQFRSDG+VVKGQ YGIRSIRVDGNDAL
Sbjct: 273 DFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDAL 332

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 290
           AVYSAVR AR MAV EQ P+LIE +TYRVGHHSTSDDS+KYRA DEIQYWKM RNPV+RF
Sbjct: 333 AVYSAVRSAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRF 392

[Close]

BLAST of Lsi08G002820 vs. ExPASy Swiss-Prot
Match: Q84JL2 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g09300 PE=1 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 5.5e-100
Identity = 190/380 (50.00%), Postives = 243/380 (63.95%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           ++FI+ES ++RVPCYRVL +NG ++T++ F+Q+ ++VA+K+Y DM+TLQ MD I YEAQR
Sbjct: 93  IQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQR 152

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGR+SFY T+ GEEAI IASAAAL P DV+  Q                           
Sbjct: 153 QGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSD 212

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 213 YGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEG 272

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALN AAV+ APV+FICRNNGWAIST   +QFRSDGVVVKG+ YGIRSIRVDGNDAL
Sbjct: 273 DFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDAL 332

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           A+YSAV  AR MA++EQ PILIEALTYRVGHHSTSDDS++YR+  EI++W   RNP+ RF
Sbjct: 333 AMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRF 392

[Close]

BLAST of Lsi08G002820 vs. ExPASy Swiss-Prot
Match: F4KIN4 (Putative 2-dehydropantoate 2-reductase OS=Arabidopsis thaliana OX=3702 GN=KPR PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.5e-76
Identity = 141/197 (71.57%), Postives = 164/197 (83.25%), Query Frame = 0


Query: 92  AQGDFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGN 151
           ++GDFHA LNFAAV+ APV+FICRNNGWAIST + EQFRSDG+VVKGQ YGIRSIRVDGN
Sbjct: 40  SEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGN 99

Query: 152 DALAVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPV 211
           DALAVYSAV  AR MAV EQ P+LIE + YRVGHHSTSDDS+KYRA DEIQYWKM RN V
Sbjct: 100 DALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHHSTSDDSTKYRAADEIQYWKMSRNSV 159

Query: 212 DRFTKWLFNNGWLNQEHDSNHKSTVRKQLLQAIQNAEKTEKPPVSELFNDVYDHLPKNLE 271
           +RF K + +NGW ++E +S  +S  RKQLLQAIQ AEK EK P++ELFNDVYD  PKNLE
Sbjct: 160 NRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLE 219

Query: 272 EQEEVLRQTIKRYPQDY 289
           E+E  L++ I++ PQDY
Sbjct: 220 EEELGLKELIEKQPQDY 236

[Close]

BLAST of Lsi08G002820 vs. ExPASy Swiss-Prot
Match: A5A6H9 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes OX=9598 GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-68
Identity = 144/377 (38.20%), Postives = 192/377 (50.93%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           + FI  +    +P YRV+   G I+  +    L K+  +K+YK M  L TMD ILYE+QR
Sbjct: 65  LEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQR 124

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFY+T+ GEE   + SAAAL   D+V  Q                           
Sbjct: 125 QGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISD 184

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 185 LGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGAASEG 244

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           D HA  NFAA L  P+IF CRNNG+AIST   EQ+R DG+  +G GYGI SIRVDGND  
Sbjct: 245 DAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVF 304

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 292
           AVY+A + AR  AV E  P LIEA+TYR+GHHSTSDDSS YR+VDE+ YW  + +PV R 
Sbjct: 305 AVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPVSRL 364

[Close]

BLAST of Lsi08G002820 vs. ExPASy Swiss-Prot
Match: P11960 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus norvegicus OX=10116 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-68
Identity = 143/377 (37.93%), Postives = 192/377 (50.93%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           + FI  +    +P YRV+   G I+  +    L ++  +K+Y+ M  L TMD ILYE+QR
Sbjct: 61  LEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPQEEVLKLYRSMTLLNTMDRILYESQR 120

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFY+T+ GEE   + SAAAL   D+V  Q                           
Sbjct: 121 QGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREAGVLMYRDYPLELFMAQCYGNVSD 180

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 181 PGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANQIVICYFGEGAASEG 240

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           D HA  NFAA L  P+IF CRNNG+AIST   EQ+R DG+  +G GYGI SIRVDGND  
Sbjct: 241 DAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVF 300

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 292
           AVY+A + AR  AV E  P LIEA+TYR+GHHSTSDDSS YR+VDE+ YW  + +P+ R 
Sbjct: 301 AVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRL 360

[Close]

BLAST of Lsi08G002820 vs. ExPASy TrEMBL
Match: A0A6J1DI76 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021170 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.1e-139
Identity = 246/294 (83.67%), Postives = 272/294 (92.52%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           M FIAESTQKRVPCYRVL +NG+ +TSNNF QL KDVA+KMYKDM+TLQTMDTI YEAQR
Sbjct: 87  MSFIAESTQKRVPCYRVLDDNGERITSNNFTQLNKDVAVKMYKDMVTLQTMDTIFYEAQR 146

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQGDFHAALNFAAVLGAPVIFICRNNGWA 120
           QGRISFYLTSSGEEAI IASAAAL  DDVVLAQGDFHAALNFAAVL APVIFICRNNGWA
Sbjct: 147 QGRISFYLTSSGEEAITIASAAALQSDDVVLAQGDFHAALNFAAVLRAPVIFICRNNGWA 206

Query: 121 ISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDALAVYSAVRRARSMAVKEQMPILIEALT 180
           ISTSV+EQFRSDGVVVKGQ YGIRSIR+DGNDALAVY+A+RRAR+MAV EQ P+L+EALT
Sbjct: 207 ISTSVEEQFRSDGVVVKGQAYGIRSIRIDGNDALAVYTAIRRARAMAVAEQAPVLVEALT 266

Query: 181 YRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRFTKWLFNNGWLNQEHDSNHKSTVRKQL 240
           YRVGHHSTSDDSSKYRA+DEIQYWKMERNPV+RF+ WL  N WL QE +SNH++TV++QL
Sbjct: 267 YRVGHHSTSDDSSKYRAMDEIQYWKMERNPVNRFSNWLTTNRWLTQEDESNHRTTVKQQL 326

Query: 241 LQAIQNAEKTEKPPVSELFNDVYDHLPKNLEEQEEVLRQTIKRYPQDYPSDVPL 295
           LQAIQNAE+ EKPPVSELF+DVYD++P NL EQE+VLRQT+KRYPQDYPSDVPL
Sbjct: 327 LQAIQNAERREKPPVSELFSDVYDNVPSNLHEQEQVLRQTMKRYPQDYPSDVPL 380

[Close]

BLAST of Lsi08G002820 vs. ExPASy TrEMBL
Match: A0A5A7V005 (2-oxoisovalerate dehydrogenase subunit alpha 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4863G00110 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.2e-134
Identity = 257/380 (67.63%), Postives = 277/380 (72.89%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           MRFI+EST+KRVPCYRVLHE GDI+T NNF QL KDVAIKMYKDMITLQ MD I YEAQR
Sbjct: 58  MRFISESTEKRVPCYRVLHETGDIITPNNFNQLTKDVAIKMYKDMITLQIMDNIFYEAQR 117

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTSSGEEAIA+ASAAALLPDDVVLAQ                           
Sbjct: 118 QGRISFYLTSSGEEAIAVASAAALLPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKRD 177

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 178 YGKGRQMPIHYGSNHHNYFTISSPLGTQLPQAVGIAYSLKMDKKDACAVAYFGDGTTSEG 237

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALNFAAVLGAPVIFICRNNGWAISTS++EQFRSDGVVVKGQGYGIRSIR+DGNDAL
Sbjct: 238 DFHAALNFAAVLGAPVIFICRNNGWAISTSIEEQFRSDGVVVKGQGYGIRSIRIDGNDAL 297

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           AVY+AV +ARSMA++E+MPILIEALTYRVGHHSTSDDSSKYR  DEIQYWKMERNPVDRF
Sbjct: 298 AVYTAVHKARSMAIREKMPILIEALTYRVGHHSTSDDSSKYRTTDEIQYWKMERNPVDRF 357

[Close]

BLAST of Lsi08G002820 vs. ExPASy TrEMBL
Match: A0A1S3BD09 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103488700 PE=4 SV=1)

HSP 1 Score: 489.2 bits (1258), Expect = 1.2e-134
Identity = 257/380 (67.63%), Postives = 277/380 (72.89%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           MRFI+EST+KRVPCYRVLHE GDI+T NNF QL KDVAIKMYKDMITLQ MD I YEAQR
Sbjct: 85  MRFISESTEKRVPCYRVLHETGDIITPNNFNQLTKDVAIKMYKDMITLQIMDNIFYEAQR 144

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTSSGEEAIA+ASAAALLPDDVVLAQ                           
Sbjct: 145 QGRISFYLTSSGEEAIAVASAAALLPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKRD 204

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 205 YGKGRQMPIHYGSNHHNYFTISSPLGTQLPQAVGIAYSLKMDKKDACAVAYFGDGTTSEG 264

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALNFAAVLGAPVIFICRNNGWAISTS++EQFRSDGVVVKGQGYGIRSIR+DGNDAL
Sbjct: 265 DFHAALNFAAVLGAPVIFICRNNGWAISTSIEEQFRSDGVVVKGQGYGIRSIRIDGNDAL 324

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           AVY+AV +ARSMA++E+MPILIEALTYRVGHHSTSDDSSKYR  DEIQYWKMERNPVDRF
Sbjct: 325 AVYTAVHKARSMAIREKMPILIEALTYRVGHHSTSDDSSKYRTTDEIQYWKMERNPVDRF 384

[Close]

BLAST of Lsi08G002820 vs. ExPASy TrEMBL
Match: A0A0A0KIX5 (E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G395130 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 1.9e-132
Identity = 253/380 (66.58%), Postives = 275/380 (72.37%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           MRF++EST+KRVPCYRVLHENGD +T  NF QL KDVAIKMYKDMITLQ MD I YEAQR
Sbjct: 86  MRFMSESTEKRVPCYRVLHENGDAITPPNFNQLTKDVAIKMYKDMITLQIMDNIFYEAQR 145

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ                           
Sbjct: 146 QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKGD 205

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 206 YGKGRQMPIHYGSNHHNYFTISSPLGTQLPQAVGIAYSLKMDKKEACVVAYFGDGTTSEG 265

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALNFAAVL APVIFICRNNGWAISTS++EQFRSDGVVVKGQGYGIRSIR+DGND L
Sbjct: 266 DFHAALNFAAVLRAPVIFICRNNGWAISTSIEEQFRSDGVVVKGQGYGIRSIRIDGNDTL 325

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           AVYSAVR+ARSMA++E MP+LIEALTYRVGHHSTSDDSSKYR  DEIQYWKMERNP+DRF
Sbjct: 326 AVYSAVRKARSMAIRENMPVLIEALTYRVGHHSTSDDSSKYRTTDEIQYWKMERNPMDRF 385

[Close]

BLAST of Lsi08G002820 vs. ExPASy TrEMBL
Match: A0A6J1FEF0 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111443206 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.7e-131
Identity = 257/381 (67.45%), Postives = 275/381 (72.18%), Query Frame = 0


Query: 1   MRFIAES-TQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQ 60
           MRFI ES TQKRVPCYRV+ E G+  TSNNF QL KD AIKMYKDMITLQT+DTI YEAQ
Sbjct: 87  MRFIGESATQKRVPCYRVIDEIGETTTSNNFTQLSKDGAIKMYKDMITLQTVDTIFYEAQ 146

Query: 61  RQGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ-------------------------- 120
           RQGRISFYLTSSGEEAI++ASAAAL PDDVVLAQ                          
Sbjct: 147 RQGRISFYLTSSGEEAISVASAAALHPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKS 206

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 207 DYGKGRQMPIHYGSIQHNYFTISSPLGTQIPQAVGIAYSLKMDKKDACAVAYFGDGTTSE 266

Query: 181 GDFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDA 240
           GDFHA LNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIR+DGNDA
Sbjct: 267 GDFHAGLNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRIDGNDA 326

Query: 241 LAVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDR 295
           LAVYSAVRRARSMAV EQMP+LIEALTYRVGHHSTSDDSSKYRAVDEI++WKMERNPV+R
Sbjct: 327 LAVYSAVRRARSMAVAEQMPVLIEALTYRVGHHSTSDDSSKYRAVDEIEHWKMERNPVNR 386

[Close]

BLAST of Lsi08G002820 vs. NCBI nr
Match: XP_038885350.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 507.3 bits (1305), Expect = 8.9e-140
Identity = 269/380 (70.79%), Postives = 282/380 (74.21%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           MRFIAESTQ+RVPCYRVLHENGD +TSNNF QL KDVAIKMYKDMITLQ MD I YEAQR
Sbjct: 83  MRFIAESTQQRVPCYRVLHENGDTITSNNFTQLSKDVAIKMYKDMITLQIMDNIFYEAQR 142

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTSSGEEAIAIASAAALLPDD+VLAQ                           
Sbjct: 143 QGRISFYLTSSGEEAIAIASAAALLPDDIVLAQYREPGVLLWRGFTLQEFANQLFGNKFD 202

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 203 YGKGRQMPIHYGSNHHNYFTISSPLGTQLPQAVAIAYSLKMDRKDACAVAYFGDGTTSEG 262

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALNFAAVLGAPVIFICRNNGWAISTSV+EQFRSDGVVVKGQGYGIRSIR+DGNDAL
Sbjct: 263 DFHAALNFAAVLGAPVIFICRNNGWAISTSVEEQFRSDGVVVKGQGYGIRSIRIDGNDAL 322

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           AVYSAVRRARSMA+ EQMPILIEALTYRVGHHSTSDDSSKYRA+DEIQYWKMERNPVDRF
Sbjct: 323 AVYSAVRRARSMAIAEQMPILIEALTYRVGHHSTSDDSSKYRAIDEIQYWKMERNPVDRF 382

[Close]

BLAST of Lsi08G002820 vs. NCBI nr
Match: XP_022153718.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 [Momordica charantia])

HSP 1 Score: 503.1 bits (1294), Expect = 1.7e-138
Identity = 246/294 (83.67%), Postives = 272/294 (92.52%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           M FIAESTQKRVPCYRVL +NG+ +TSNNF QL KDVA+KMYKDM+TLQTMDTI YEAQR
Sbjct: 87  MSFIAESTQKRVPCYRVLDDNGERITSNNFTQLNKDVAVKMYKDMVTLQTMDTIFYEAQR 146

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQGDFHAALNFAAVLGAPVIFICRNNGWA 120
           QGRISFYLTSSGEEAI IASAAAL  DDVVLAQGDFHAALNFAAVL APVIFICRNNGWA
Sbjct: 147 QGRISFYLTSSGEEAITIASAAALQSDDVVLAQGDFHAALNFAAVLRAPVIFICRNNGWA 206

Query: 121 ISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDALAVYSAVRRARSMAVKEQMPILIEALT 180
           ISTSV+EQFRSDGVVVKGQ YGIRSIR+DGNDALAVY+A+RRAR+MAV EQ P+L+EALT
Sbjct: 207 ISTSVEEQFRSDGVVVKGQAYGIRSIRIDGNDALAVYTAIRRARAMAVAEQAPVLVEALT 266

Query: 181 YRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRFTKWLFNNGWLNQEHDSNHKSTVRKQL 240
           YRVGHHSTSDDSSKYRA+DEIQYWKMERNPV+RF+ WL  N WL QE +SNH++TV++QL
Sbjct: 267 YRVGHHSTSDDSSKYRAMDEIQYWKMERNPVNRFSNWLTTNRWLTQEDESNHRTTVKQQL 326

Query: 241 LQAIQNAEKTEKPPVSELFNDVYDHLPKNLEEQEEVLRQTIKRYPQDYPSDVPL 295
           LQAIQNAE+ EKPPVSELF+DVYD++P NL EQE+VLRQT+KRYPQDYPSDVPL
Sbjct: 327 LQAIQNAERREKPPVSELFSDVYDNVPSNLHEQEQVLRQTMKRYPQDYPSDVPL 380

[Close]

BLAST of Lsi08G002820 vs. NCBI nr
Match: XP_008445779.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Cucumis melo])

HSP 1 Score: 489.2 bits (1258), Expect = 2.5e-134
Identity = 257/380 (67.63%), Postives = 277/380 (72.89%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           MRFI+EST+KRVPCYRVLHE GDI+T NNF QL KDVAIKMYKDMITLQ MD I YEAQR
Sbjct: 85  MRFISESTEKRVPCYRVLHETGDIITPNNFNQLTKDVAIKMYKDMITLQIMDNIFYEAQR 144

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTSSGEEAIA+ASAAALLPDDVVLAQ                           
Sbjct: 145 QGRISFYLTSSGEEAIAVASAAALLPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKRD 204

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 205 YGKGRQMPIHYGSNHHNYFTISSPLGTQLPQAVGIAYSLKMDKKDACAVAYFGDGTTSEG 264

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALNFAAVLGAPVIFICRNNGWAISTS++EQFRSDGVVVKGQGYGIRSIR+DGNDAL
Sbjct: 265 DFHAALNFAAVLGAPVIFICRNNGWAISTSIEEQFRSDGVVVKGQGYGIRSIRIDGNDAL 324

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           AVY+AV +ARSMA++E+MPILIEALTYRVGHHSTSDDSSKYR  DEIQYWKMERNPVDRF
Sbjct: 325 AVYTAVHKARSMAIREKMPILIEALTYRVGHHSTSDDSSKYRTTDEIQYWKMERNPVDRF 384

[Close]

BLAST of Lsi08G002820 vs. NCBI nr
Match: KAA0059796.1 (2-oxoisovalerate dehydrogenase subunit alpha 1 [Cucumis melo var. makuwa] >TYK05839.1 2-oxoisovalerate dehydrogenase subunit alpha 1 [Cucumis melo var. makuwa])

HSP 1 Score: 489.2 bits (1258), Expect = 2.5e-134
Identity = 257/380 (67.63%), Postives = 277/380 (72.89%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           MRFI+EST+KRVPCYRVLHE GDI+T NNF QL KDVAIKMYKDMITLQ MD I YEAQR
Sbjct: 58  MRFISESTEKRVPCYRVLHETGDIITPNNFNQLTKDVAIKMYKDMITLQIMDNIFYEAQR 117

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTSSGEEAIA+ASAAALLPDDVVLAQ                           
Sbjct: 118 QGRISFYLTSSGEEAIAVASAAALLPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKRD 177

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 178 YGKGRQMPIHYGSNHHNYFTISSPLGTQLPQAVGIAYSLKMDKKDACAVAYFGDGTTSEG 237

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALNFAAVLGAPVIFICRNNGWAISTS++EQFRSDGVVVKGQGYGIRSIR+DGNDAL
Sbjct: 238 DFHAALNFAAVLGAPVIFICRNNGWAISTSIEEQFRSDGVVVKGQGYGIRSIRIDGNDAL 297

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           AVY+AV +ARSMA++E+MPILIEALTYRVGHHSTSDDSSKYR  DEIQYWKMERNPVDRF
Sbjct: 298 AVYTAVHKARSMAIREKMPILIEALTYRVGHHSTSDDSSKYRTTDEIQYWKMERNPVDRF 357

[Close]

BLAST of Lsi08G002820 vs. NCBI nr
Match: XP_011657437.1 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Cucumis sativus] >KGN47731.1 hypothetical protein Csa_018237 [Cucumis sativus])

HSP 1 Score: 481.9 bits (1239), Expect = 4.0e-132
Identity = 253/380 (66.58%), Postives = 275/380 (72.37%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           MRF++EST+KRVPCYRVLHENGD +T  NF QL KDVAIKMYKDMITLQ MD I YEAQR
Sbjct: 86  MRFMSESTEKRVPCYRVLHENGDAITPPNFNQLTKDVAIKMYKDMITLQIMDNIFYEAQR 145

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ                           
Sbjct: 146 QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQYREPGVLLWRGFTLQEFANQLFGNKGD 205

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 206 YGKGRQMPIHYGSNHHNYFTISSPLGTQLPQAVGIAYSLKMDKKEACVVAYFGDGTTSEG 265

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALNFAAVL APVIFICRNNGWAISTS++EQFRSDGVVVKGQGYGIRSIR+DGND L
Sbjct: 266 DFHAALNFAAVLRAPVIFICRNNGWAISTSIEEQFRSDGVVVKGQGYGIRSIRIDGNDTL 325

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           AVYSAVR+ARSMA++E MP+LIEALTYRVGHHSTSDDSSKYR  DEIQYWKMERNP+DRF
Sbjct: 326 AVYSAVRKARSMAIRENMPVLIEALTYRVGHHSTSDDSSKYRTTDEIQYWKMERNPMDRF 385

[Close]

BLAST of Lsi08G002820 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 386.0 bits (990), Expect = 2.8e-107
Identity = 208/375 (55.47%), Postives = 242/375 (64.53%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           M+FI ES+ +R+PCYRVL E+G I+  ++FI + + +A++MY+ M TLQ MD I YEAQR
Sbjct: 93  MKFIPESSSRRIPCYRVLDEDGRIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQR 152

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGRISFYLTS GEEAI IASAAAL PDDVVL Q                           
Sbjct: 153 QGRISFYLTSVGEEAINIASAAALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKAD 212

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 213 YGKGRQMPIHYGSNRLNYFTISSPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEG 272

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHA LNFAAV+ APV+FICRNNGWAIST + EQFRSDG+VVKGQ YGIRSIRVDGNDAL
Sbjct: 273 DFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDAL 332

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 290
           AVYSAVR AR MAV EQ P+LIE +TYRVGHHSTSDDS+KYRA DEIQYWKM RNPV+RF
Sbjct: 333 AVYSAVRSAREMAVTEQRPVLIEMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRF 392

[Close]

BLAST of Lsi08G002820 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 3.9e-101
Identity = 190/380 (50.00%), Postives = 243/380 (63.95%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           ++FI+ES ++RVPCYRVL +NG ++T++ F+Q+ ++VA+K+Y DM+TLQ MD I YEAQR
Sbjct: 93  IQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQR 152

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGR+SFY T+ GEEAI IASAAAL P DV+  Q                           
Sbjct: 153 QGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSD 212

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 213 YGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEG 272

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALN AAV+ APV+FICRNNGWAIST   +QFRSDGVVVKG+ YGIRSIRVDGNDAL
Sbjct: 273 DFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDAL 332

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           A+YSAV  AR MA++EQ PILIEALTYRVGHHSTSDDS++YR+  EI++W   RNP+ RF
Sbjct: 333 AMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRF 392

[Close]

BLAST of Lsi08G002820 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 365.5 bits (937), Expect = 3.9e-101
Identity = 190/380 (50.00%), Postives = 243/380 (63.95%), Query Frame = 0


Query: 1   MRFIAESTQKRVPCYRVLHENGDILTSNNFIQLRKDVAIKMYKDMITLQTMDTILYEAQR 60
           ++FI+ES ++RVPCYRVL +NG ++T++ F+Q+ ++VA+K+Y DM+TLQ MD I YEAQR
Sbjct: 22  IQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQR 81

Query: 61  QGRISFYLTSSGEEAIAIASAAALLPDDVVLAQ--------------------------- 120
           QGR+SFY T+ GEEAI IASAAAL P DV+  Q                           
Sbjct: 82  QGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSD 141

Query: 121 -----------------------------------------------------------G 180
                                                                      G
Sbjct: 142 YGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEG 201

Query: 181 DFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGNDAL 240
           DFHAALN AAV+ APV+FICRNNGWAIST   +QFRSDGVVVKG+ YGIRSIRVDGNDAL
Sbjct: 202 DFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDAL 261

Query: 241 AVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPVDRF 295
           A+YSAV  AR MA++EQ PILIEALTYRVGHHSTSDDS++YR+  EI++W   RNP+ RF
Sbjct: 262 AMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRF 321

[Close]

BLAST of Lsi08G002820 vs. TAIR 10
Match: AT5G34780.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 1.0e-77
Identity = 141/197 (71.57%), Postives = 164/197 (83.25%), Query Frame = 0


Query: 92  AQGDFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKGQGYGIRSIRVDGN 151
           ++GDFHA LNFAAV+ APV+FICRNNGWAIST + EQFRSDG+VVKGQ YGIRSIRVDGN
Sbjct: 40  SEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGN 99

Query: 152 DALAVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAVDEIQYWKMERNPV 211
           DALAVYSAV  AR MAV EQ P+LIE + YRVGHHSTSDDS+KYRA DEIQYWKM RN V
Sbjct: 100 DALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHHSTSDDSTKYRAADEIQYWKMSRNSV 159

Query: 212 DRFTKWLFNNGWLNQEHDSNHKSTVRKQLLQAIQNAEKTEKPPVSELFNDVYDHLPKNLE 271
           +RF K + +NGW ++E +S  +S  RKQLLQAIQ AEK EK P++ELFNDVYD  PKNLE
Sbjct: 160 NRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLE 219

Query: 272 EQEEVLRQTIKRYPQDY 289
           E+E  L++ I++ PQDY
Sbjct: 220 EEELGLKELIEKQPQDY 236

[Close]

BLAST of Lsi08G002820 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-15
Identity = 52/185 (28.11%), Postives = 87/185 (47.03%), Query Frame = 0


Query: 87  DDVVLA--------QGDFHAALNFAAVLGAPVIFICRNNGWAISTSVQEQFRSDGVVVKG 146
           DDV +A         G F   LN AA+   P+IF+  NN WAI  S         +  KG
Sbjct: 218 DDVTVAFFGDGTCNNGQFFECLNMAALYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKG 277

Query: 147 QGYGIRSIRVDGNDALAVYSAVRRARSMAVKEQMPILIEALTYRVGHHSTSDDSSKYRAV 206
             +G+  + VDG D L V    + A + A + + P L+E  TYR   HS +D      A 
Sbjct: 278 PAFGMPGVHVDGMDVLKVREVAKEAVTRARRGEGPTLVECETYRFRGHSLADPDELRDAA 337

Query: 207 DEIQYWKMERNPVDRFTKWLFNNGWLNQEHDSNHKSTVRKQLLQAIQNAEKTEKPPVSEL 264
           ++ +Y    R+P+    K+L  N    +    + +  + + + +A++ A+ + +P  S+L
Sbjct: 338 EKAKY--AARDPIAALKKYLIENKLAKEAELKSIEKKIDELVEEAVEFADASPQPGRSQL 397

The following BLAST results are available for this feature:

BLAST of Lsi08G002820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LPL5	3.9e-106	55.47	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis tha...	[more]
Q84JL2	5.5e-100	50.00	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis tha...	[more]
F4KIN4	1.5e-76	71.57	Putative 2-dehydropantoate 2-reductase OS=Arabidopsis thaliana OX=3702 GN=KPR PE...	[more]
A5A6H9	1.5e-68	38.20	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes O...	[more]
P11960	1.5e-68	37.93	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (Fragment) OS=Rattus...	[more]

BLAST of Lsi08G002820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DI76	8.1e-139	83.67	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 OS...	[more]
A0A5A7V005	1.2e-134	67.63	2-oxoisovalerate dehydrogenase subunit alpha 1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BD09	1.2e-134	67.63	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Cucumis melo OX...	[more]
A0A0A0KIX5	1.9e-132	66.58	E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G395130 PE=4 ...	[more]
A0A6J1FEF0	3.7e-131	67.45	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like OS=Cucurbita ...	[more]

BLAST of Lsi08G002820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038885350.1	8.9e-140	70.79	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Benincasa hispida...	[more]
XP_022153718.1	1.7e-138	83.67	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial-like isoform X2 [M...	[more]
XP_008445779.1	2.5e-134	67.63	PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Cucumi...	[more]
KAA0059796.1	2.5e-134	67.63	2-oxoisovalerate dehydrogenase subunit alpha 1 [Cucumis melo var. makuwa] >TYK05...	[more]
XP_011657437.1	4.0e-132	66.58	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial [Cucumis sativus] ...	[more]

BLAST of Lsi08G002820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21400.1	2.8e-107	55.47	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.1	3.9e-101	50.00	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.2	3.9e-101	50.00	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G34780.1	1.0e-77	71.57	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT1G01090.1	1.1e-15	28.11	pyruvate dehydrogenase E1 alpha	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.970		coord: 92..292 e-value: 4.8E-69 score: 235.1 coord: 1..91 e-value: 5.1E-25 score: 90.2
None	No IPR available	PANTHER	PTHR43380:SF11	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL	coord: 93..294
None	No IPR available	PANTHER	PTHR43380:SF11	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL	coord: 1..93
None	No IPR available	PANTHER	PTHR43380	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL	coord: 93..294 coord: 1..93
None	No IPR available	CDD	cd02000	TPP_E1_PDC_ADC_BCADC	coord: 41..247 e-value: 5.10343E-76 score: 232.001
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 92..255 e-value: 2.6E-56 score: 190.8
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 12..289

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G002820.1	Lsi08G002820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009083	branched-chain amino acid catabolic process
cellular_component	GO:0005947	mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor