Lsi08G002560 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G002560
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Protein kinase superfamily protein
Location	chr08: 5754273 .. 5756671 (-)
RNA-Seq Expression	Lsi08G002560
Synteny	Lsi08G002560

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCAGAGTATAAGAGAAGACACAATCGTAGATGAAGAAGGAGCATATGTTCCTTTAATATTGCACTTGCAAATCTCCAAGAACTTCAATACTTTGTGAATCCATTTTCTTTTTTTCTTCCGTTGCTTCATTGCTGAAACAAACAAAAAGTTTGAGAAATCATGGGAGCCGAGAAGATCTACAATGGCTCCGTGGATGTGGTAGAAGCTGTAGGAGATGGAGATGGCGATGGCAACACCGAGTCGTATCAGTCATCGACTCTTTCCGTCGACCTCTCTCTTCCTCTTCCCGCCATGACCCCTCGCATAATGTTCGTATCGCTTCCTCTTTCCTTTCCTTTCTTCTTTCCACATATGGATTGTTTATGCTTCCTATTTTTGCTTTTTTCTGTTCTGTTTTTCCATAAACGGGAAGCAAGACGACCAGAGTGATTCTTGTTTTTTTGAATTTGATGATTTCATCTCCTTGCTTCTCGGAGCTCGCGGTATGCGGTTACTGAAGCTAATTCTGGAAGTTTCTTTCGTTGATTTTGTTCTCAGTCGTTTTCTTGATTTTCTTGCAGCGAGCTGTGTAAGGATCTGTTCAAGGAGTGGTCGGAGCTGGACGATTCTCGGTTCTCTGTTGAAACGGTCTCTGGTGGTATCACTAATCAGCGTGAGTGTTTGCTTACAATGTATTTCTTCGTTCAATTAGATTAACGCGAGACTGTGTTCGAGGTTGCGGTTACTGATTTCTTATGCTTGTGGAGTTCTGTTCTGTAACAGGAATTTGTTTTTTCTGACTTCTGCAGTGCTTAAGGTTACGGTGAAAGAGGAAAGTGGTAGTTACGTTTCTGTCACCGTCAGACTATATGGACCTAACACGGATTATGTTATTAATCGTCACAGGGAACTGCAGGTGACTTTCGAGGATAGATTATTGAGTGTTAAATTATCCGTTTGTTGTGTACGCTAAGTGTAGTCTGTGTTCTTTACGTCGTCTTCTTTCTTTTTCCCTCTGTCTTGGCGGGCAACGGTATTTCATCTTTTAGCATAGTCAGCCCTAAAACAATCTAGTCATGTTCTTTGTAAATCGAATCTGACCATTCTTAAGGTGATATATGCATGGCTCTAGTACTTAGCAATTCGATATTGATTCCTATTCACCTATACTATTACGTTTAATCAAAGTACGTAATAAGTAAGCTAAACTCAGCTATGTTTGAAAATATTCTCATGTTGTATATTTGGTGTTCGTTCATGTGTGCATGACAATCAAGAACTGTAGCTTTGACATAGAAGAAATCTTTCGTGCTCAAACCTCATCTATATTGGTTCAGAATTCTAATATATTAATATATTTCTTTTACCATTAGCATCTTAGTATTGATCCAATATTTTCAGTATTGGTTATTTATTATTATCATTGTTGTTGTTTTGTGAATGATCTGATCAGCGAAATTGTCTTTGGTGTTCTGGGTGGTGTATGTACAGGTGTTAAGGTAGGGAGTATTCTGTTAATTTCTGTTTTATGCTCTTAAAAAAAATCCGTTTTATGGGTGTATACAAGTAGTATTGAAGACTTATTCTTTGTAATCAATTCACATTTGTTAAGGAGAATGGCAATTGTTTTTCTGTCTGTAATCGAGTCTATATTATCTGCTTTGTGAGGGAAATCATGTTCCTGACAGAGAGAGCGCTCATTGCCTTGGGTAGGATCTTGTGACTTTTAAGATTTTGATACTTGATTGAACTCGTTGAGGTCTCTTAGAGGGTGTGGTGTAGGCCTTACAGCTGAATCTCTTAAAAGTCTTTATCTTCTTTCTCTCTTCCACCACTTCCTTGTCTTTTGTTCCATTTTACTTTCTTTAGTTTTTTCCCACTAGTATTATGGTTTTATCTTCCTTACTTGCATGGTCTTGAACCAAGAGGATTCAGGAAAGTCTATGTGGATTCGTGAAATCTAGAGTGCAATCTGTATAACCAGACCATGTATTTTTTTATTTTTTTATTTTTTATAATTTTTATCTTGAATTTGATTATTCAAATAATGCTACTAATTGTTTATCTTTCACTTGGCTTCCCCTCCCTTTTCGTGTGTGAATTTTGCTTATATCATGTTATTCACTCTGCAATCTCATGAAATCTTGTATTAGGCAATCAAATATCTTTCAGCTGCAGGATTTGGTGCGAAGCTTCTTGGAGTTTTTAAAAATGGAATGGTGCAGTCATTTATTCATGCACGTACGCTAGAACCATCAGGTAAAAAAGCATTTAGAATCTTTCTGATTTACACCTTATTTGCAGACAATTCAATTCTAATCTTTAATATTGAATGTTGTTGCATTATTATGTAGCATTTCATTCTAATCATGGTTGCACTGAAAGCTTTGCAGTAAAGATCTATGGAGAGAGAGAATGA

mRNA sequence

GGCAGAGTATAAGAGAAGACACAATCGTAGATGAAGAAGGAGCATATGTTCCTTTAATATTGCACTTGCAAATCTCCAAGAACTTCAATACTTTGTGAATCCATTTTCTTTTTTTCTTCCGTTGCTTCATTGCTGAAACAAACAAAAAGTTTGAGAAATCATGGGAGCCGAGAAGATCTACAATGGCTCCGTGGATGTGGTAGAAGCTGTAGGAGATGGAGATGGCGATGGCAACACCGAGTCGTATCAGTCATCGACTCTTTCCGTCGACCTCTCTCTTCCTCTTCCCGCCATGACCCCTCGCATAATCGAGCTGTGTAAGGATCTGTTCAAGGAGTGGTCGGAGCTGGACGATTCTCGGTTCTCTGTTGAAACGGTCTCTGGTGGTATCACTAATCAGCGTGAGTGTTTGCTTACAATGAATTTGTTTTTTCTGACTTCTGCAGTGCTTAAGGTTACGGTGAAAGAGGAAAGTGGTAGTTACGTTTCTGTCACCGTCAGACTATATGGACCTAACACGGATTATGTTATTAATCGTCACAGGGAACTGCAGGCAATCAAATATCTTTCAGCTGCAGGATTTGGTGCGAAGCTTCTTGGAGTTTTTAAAAATGGAATGGTGCAGTCATTTATTCATGCACGTACGCTAGAACCATCAGCATTTCATTCTAATCATGGTTGCACTGAAAGCTTTGCAGTAAAGATCTATGGAGAGAGAGAATGA

Coding sequence (CDS)

ATGGGAGCCGAGAAGATCTACAATGGCTCCGTGGATGTGGTAGAAGCTGTAGGAGATGGAGATGGCGATGGCAACACCGAGTCGTATCAGTCATCGACTCTTTCCGTCGACCTCTCTCTTCCTCTTCCCGCCATGACCCCTCGCATAATCGAGCTGTGTAAGGATCTGTTCAAGGAGTGGTCGGAGCTGGACGATTCTCGGTTCTCTGTTGAAACGGTCTCTGGTGGTATCACTAATCAGCGTGAGTGTTTGCTTACAATGAATTTGTTTTTTCTGACTTCTGCAGTGCTTAAGGTTACGGTGAAAGAGGAAAGTGGTAGTTACGTTTCTGTCACCGTCAGACTATATGGACCTAACACGGATTATGTTATTAATCGTCACAGGGAACTGCAGGCAATCAAATATCTTTCAGCTGCAGGATTTGGTGCGAAGCTTCTTGGAGTTTTTAAAAATGGAATGGTGCAGTCATTTATTCATGCACGTACGCTAGAACCATCAGCATTTCATTCTAATCATGGTTGCACTGAAAGCTTTGCAGTAAAGATCTATGGAGAGAGAGAATGA

Protein sequence

MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEWSELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNTDYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPSAFHSNHGCTESFAVKIYGERE

Homology

[Close]

BLAST of Lsi08G002560 vs. ExPASy Swiss-Prot
Match: O81024 (Probable ethanolamine kinase OS=Arabidopsis thaliana OX=3702 GN=EMB1187 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.6e-44
Identity = 97/146 (66.44%), Postives = 106/146 (72.60%), Query Frame = 0


Query: 21  DGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEWSELDDSRFSVETVSGGITNQ 80
           D   + E    S+  VD SLPLP M PRIIELCKDLFK W ELDDS FSVE VSGGITN 
Sbjct: 15  DAANDAEQIPYSSFVVDTSLPLPLMIPRIIELCKDLFKNWGELDDSLFSVERVSGGITN- 74

Query: 81  RECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNTDYVINRHRELQAIKYLSAAG 140
                          +LKV+VKE++   VSVTVRLYGPNT+YVINR RE+ AIKYLSAAG
Sbjct: 75  --------------LLLKVSVKEDTNKEVSVTVRLYGPNTEYVINREREILAIKYLSAAG 134

Query: 141 FGAKLLGVFKNGMVQSFIHARTLEPS 167
           FGAKLLG F NGMVQSFI+ARTLEPS
Sbjct: 135 FGAKLLGGFGNGMVQSFINARTLEPS 145

[Close]

BLAST of Lsi08G002560 vs. ExPASy Swiss-Prot
Match: Q8L518 (Probable choline kinase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g74320 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.9e-07
Identity = 34/115 (29.57%), Postives = 55/115 (47.83%), Query Frame = 0


Query: 51  ELCKDLFKEWSELDDSR-FSVETVSGGITNQRECLLTMNLFFLTSAVLKVT-VKEESGSY 110
           E  + +  EW ++ DS+   V  + G +TN+               V ++     E G  
Sbjct: 23  EALQAIASEWEDVIDSKALQVIPLKGAMTNE---------------VFQIKWPTREKGPS 82

Query: 111 VSVTVRLYGPNTDYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTL 164
             V VR+YG   +   +R  E++  +++S  G G  LLG F NG ++ F+HARTL
Sbjct: 83  RKVLVRIYGEGVEIFFDREDEIRTFEFMSKHGHGPLLLGRFGNGRIEEFLHARTL 122

[Close]

BLAST of Lsi08G002560 vs. ExPASy Swiss-Prot
Match: Q9M9H6 (Probable choline kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CK1 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.3e-06
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 92  LTSAVLKVTVKEESGSYV--SVTVRLYGPNTDYVINRHRELQAIKYLSAAGFGAKLLGVF 151
           +T+ V ++     +G  V   V VR+YG   D   NR  E++  + +S  G+G KLLG F
Sbjct: 49  MTNEVYQINWPTLNGEDVHRKVLVRIYGDGVDLFFNRGDEIKTFECMSHHGYGPKLLGRF 108

Query: 152 KNGMVQSFIHARTL 164
            +G ++ FIHARTL
Sbjct: 109 SDGRLEEFIHARTL 122

[Close]

BLAST of Lsi08G002560 vs. ExPASy Swiss-Prot
Match: P20485 (Choline kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CKI1 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 3.8e-06
Identity = 29/78 (37.18%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 87  MNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNTDYVINRHRELQAIKYLSAAGFGAKLL 146
           MNL  ++ A+     K E     S+ +R+YGPN D +I+R  ELQ +  LS    G  L 
Sbjct: 148 MNLVKISGAMTNAIFKVEYPKLPSLLLRIYGPNIDNIIDREYELQILARLSLKNIGPSLY 207

Query: 147 GVFKNGMVQSFI-HARTL 164
           G F NG  + F+ +++TL
Sbjct: 208 GCFVNGRFEQFLENSKTL 225

[Close]

BLAST of Lsi08G002560 vs. ExPASy Swiss-Prot
Match: Q869T9 (Probable ethanolamine kinase A OS=Dictyostelium discoideum OX=44689 GN=etnkA PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 8.5e-06
Identity = 30/115 (26.09%), Postives = 51/115 (44.35%), Query Frame = 0


Query: 49  IIELCKDLFKEWSELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSY 108
           + ++ +    E+    D   +++ ++GGITN    +   N+             E+   Y
Sbjct: 25  LCDIARYFVPEYRNSKDEDLTIQKLNGGITNVLYLVEDKNI-------------EQKYRY 84

Query: 109 VSVTVRLYGPNTDYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTL 164
           + V +RLYG  ++ +I+R  EL         G GAK  G+F NG +  FI    L
Sbjct: 85  LPVVIRLYGYKSEEIIDRKNELIIQTEADQNGLGAKFYGLFDNGCIYGFIKGEPL 126

[Close]

BLAST of Lsi08G002560 vs. ExPASy TrEMBL
Match: A0A6J1J1G4 (probable ethanolamine kinase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481004 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.1e-63
Identity = 135/166 (81.33%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGAEKIYNGSVD+  AV  GDG+GN ESY+ STLSVDLSLPLPAMTP IIELCKDLFKEW
Sbjct: 1   MGAEKIYNGSVDLEGAV--GDGEGNPESYRLSTLSVDLSLPLPAMTPHIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD+SRFSVETVSGGITN                +LKVTVKEESGS VSVTVRLYGPNT
Sbjct: 61  SELDNSRFSVETVSGGITN---------------LLLKVTVKEESGSCVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGA+LLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAELLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. ExPASy TrEMBL
Match: A0A6J1J3G7 (probable ethanolamine kinase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481004 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.1e-63
Identity = 135/166 (81.33%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGAEKIYNGSVD+  AV  GDG+GN ESY+ STLSVDLSLPLPAMTP IIELCKDLFKEW
Sbjct: 1   MGAEKIYNGSVDLEGAV--GDGEGNPESYRLSTLSVDLSLPLPAMTPHIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD+SRFSVETVSGGITN                +LKVTVKEESGS VSVTVRLYGPNT
Sbjct: 61  SELDNSRFSVETVSGGITN---------------LLLKVTVKEESGSCVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGA+LLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAELLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. ExPASy TrEMBL
Match: A0A5D3D4J8 (Putative ethanolamine kinase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold607G00090 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.5e-63
Identity = 136/166 (81.93%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGA+KIYNG VDVVEAV   DG  N E YQ STLSVDLSLPLPAMTPRIIELCKDLFKEW
Sbjct: 1   MGAKKIYNGFVDVVEAV--EDGHSNAEPYQLSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD SRFSVETVSGGITNQ               +LKVTVKEESG+ VSVTVRLYGPNT
Sbjct: 61  SELDASRFSVETVSGGITNQ---------------LLKVTVKEESGTSVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. ExPASy TrEMBL
Match: A0A1S3CIA0 (probable ethanolamine kinase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501288 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.5e-63
Identity = 136/166 (81.93%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGA+KIYNG VDVVEAV   DG  N E YQ STLSVDLSLPLPAMTPRIIELCKDLFKEW
Sbjct: 1   MGAKKIYNGFVDVVEAV--EDGHSNAEPYQLSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD SRFSVETVSGGITNQ               +LKVTVKEESG+ VSVTVRLYGPNT
Sbjct: 61  SELDASRFSVETVSGGITNQ---------------LLKVTVKEESGTSVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. ExPASy TrEMBL
Match: A0A0A0KDN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G018610 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.0e-62
Identity = 135/166 (81.33%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGA+KIYNG VDVVEAV   DGD N ESYQ S LSVD SLPLPA+TPRIIELCKDLFKEW
Sbjct: 1   MGAKKIYNGDVDVVEAV--EDGDSNAESYQLSYLSVDHSLPLPAITPRIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD SRFSVETVSGGITNQ               +LKVTVKEESG+ VSVTVRLYGPNT
Sbjct: 61  SELDASRFSVETVSGGITNQ---------------LLKVTVKEESGTSVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. NCBI nr
Match: XP_038886169.1 (probable ethanolamine kinase [Benincasa hispida])

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-66
Identity = 138/166 (83.13%), Postives = 143/166 (86.14%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGAEKI NG  DVVE VGD DGDGNTESYQ STLSVDLSLPLPAMTPRIIELCKDLFKEW
Sbjct: 1   MGAEKICNGCTDVVEVVGDEDGDGNTESYQLSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD SRFSVETVSGGITN+               +LKVTV+EESG+YVSVTVRLYGPNT
Sbjct: 61  SELDGSRFSVETVSGGITNK---------------LLKVTVEEESGTYVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVI+R RELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVIDRDRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 151

[Close]

BLAST of Lsi08G002560 vs. NCBI nr
Match: XP_022982034.1 (probable ethanolamine kinase isoform X1 [Cucurbita maxima])

HSP 1 Score: 251.9 bits (642), Expect = 4.3e-63
Identity = 135/166 (81.33%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGAEKIYNGSVD+  AV  GDG+GN ESY+ STLSVDLSLPLPAMTP IIELCKDLFKEW
Sbjct: 1   MGAEKIYNGSVDLEGAV--GDGEGNPESYRLSTLSVDLSLPLPAMTPHIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD+SRFSVETVSGGITN                +LKVTVKEESGS VSVTVRLYGPNT
Sbjct: 61  SELDNSRFSVETVSGGITN---------------LLLKVTVKEESGSCVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGA+LLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAELLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. NCBI nr
Match: XP_023521006.1 (probable ethanolamine kinase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 251.9 bits (642), Expect = 4.3e-63
Identity = 135/166 (81.33%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGAEKIYNGS+D+  AV  GDG+GN ESY+ STLSVDLSLPLPAMTPRIIELCKDLF EW
Sbjct: 1   MGAEKIYNGSLDLGGAV--GDGEGNPESYRLSTLSVDLSLPLPAMTPRIIELCKDLFNEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD+SRFSVETVSGGITN                +LKVTVKEESGS VSVTVRLYGPNT
Sbjct: 61  SELDNSRFSVETVSGGITN---------------LLLKVTVKEESGSCVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. NCBI nr
Match: XP_022982035.1 (probable ethanolamine kinase isoform X2 [Cucurbita maxima])

HSP 1 Score: 251.9 bits (642), Expect = 4.3e-63
Identity = 135/166 (81.33%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGAEKIYNGSVD+  AV  GDG+GN ESY+ STLSVDLSLPLPAMTP IIELCKDLFKEW
Sbjct: 1   MGAEKIYNGSVDLEGAV--GDGEGNPESYRLSTLSVDLSLPLPAMTPHIIELCKDLFKEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD+SRFSVETVSGGITN                +LKVTVKEESGS VSVTVRLYGPNT
Sbjct: 61  SELDNSRFSVETVSGGITN---------------LLLKVTVKEESGSCVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGA+LLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAELLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. NCBI nr
Match: KAG6607620.1 (putative ethanolamine kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 251.9 bits (642), Expect = 4.3e-63
Identity = 135/166 (81.33%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MGAEKIYNGSVDVVEAVGDGDGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEW 60
           MGAEKIYNGS+D+  AV  GDG+GN ESY+ STLSVDLSLPLPAMTPRIIELCKDLF EW
Sbjct: 1   MGAEKIYNGSLDLGGAV--GDGEGNPESYRLSTLSVDLSLPLPAMTPRIIELCKDLFNEW 60

Query: 61  SELDDSRFSVETVSGGITNQRECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNT 120
           SELD+SRFSVETVSGGITN                +LKVTVKEESGS VSVTVRLYGPNT
Sbjct: 61  SELDNSRFSVETVSGGITN---------------LLLKVTVKEESGSCVSVTVRLYGPNT 120

Query: 121 DYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 167
           DYVINR RELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS
Sbjct: 121 DYVINRDRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTLEPS 149

[Close]

BLAST of Lsi08G002560 vs. TAIR 10
Match: AT2G26830.1 (Protein kinase superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.1e-45
Identity = 97/146 (66.44%), Postives = 106/146 (72.60%), Query Frame = 0


Query: 21  DGDGNTESYQSSTLSVDLSLPLPAMTPRIIELCKDLFKEWSELDDSRFSVETVSGGITNQ 80
           D   + E    S+  VD SLPLP M PRIIELCKDLFK W ELDDS FSVE VSGGITN 
Sbjct: 15  DAANDAEQIPYSSFVVDTSLPLPLMIPRIIELCKDLFKNWGELDDSLFSVERVSGGITN- 74

Query: 81  RECLLTMNLFFLTSAVLKVTVKEESGSYVSVTVRLYGPNTDYVINRHRELQAIKYLSAAG 140
                          +LKV+VKE++   VSVTVRLYGPNT+YVINR RE+ AIKYLSAAG
Sbjct: 75  --------------LLLKVSVKEDTNKEVSVTVRLYGPNTEYVINREREILAIKYLSAAG 134

Query: 141 FGAKLLGVFKNGMVQSFIHARTLEPS 167
           FGAKLLG F NGMVQSFI+ARTLEPS
Sbjct: 135 FGAKLLGGFGNGMVQSFINARTLEPS 145

[Close]

BLAST of Lsi08G002560 vs. TAIR 10
Match: AT1G74320.1 (Protein kinase superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 4.2e-08
Identity = 34/115 (29.57%), Postives = 55/115 (47.83%), Query Frame = 0


Query: 51  ELCKDLFKEWSELDDSR-FSVETVSGGITNQRECLLTMNLFFLTSAVLKVT-VKEESGSY 110
           E  + +  EW ++ DS+   V  + G +TN+               V ++     E G  
Sbjct: 23  EALQAIASEWEDVIDSKALQVIPLKGAMTNE---------------VFQIKWPTREKGPS 82

Query: 111 VSVTVRLYGPNTDYVINRHRELQAIKYLSAAGFGAKLLGVFKNGMVQSFIHARTL 164
             V VR+YG   +   +R  E++  +++S  G G  LLG F NG ++ F+HARTL
Sbjct: 83  RKVLVRIYGEGVEIFFDREDEIRTFEFMSKHGHGPLLLGRFGNGRIEEFLHARTL 122

[Close]

BLAST of Lsi08G002560 vs. TAIR 10
Match: AT1G71697.1 (choline kinase 1 )

HSP 1 Score: 54.7 bits (130), Expect = 9.4e-08
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 92  LTSAVLKVTVKEESGSYV--SVTVRLYGPNTDYVINRHRELQAIKYLSAAGFGAKLLGVF 151
           +T+ V ++     +G  V   V VR+YG   D   NR  E++  + +S  G+G KLLG F
Sbjct: 49  MTNEVYQINWPTLNGEDVHRKVLVRIYGDGVDLFFNRGDEIKTFECMSHHGYGPKLLGRF 108

Query: 152 KNGMVQSFIHARTL 164
            +G ++ FIHARTL
Sbjct: 109 SDGRLEEFIHARTL 122

The following BLAST results are available for this feature:

BLAST of Lsi08G002560 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81024	1.6e-44	66.44	Probable ethanolamine kinase OS=Arabidopsis thaliana OX=3702 GN=EMB1187 PE=2 SV=...	[more]
Q8L518	5.9e-07	29.57	Probable choline kinase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g74320 PE=2 SV=1	[more]
Q9M9H6	1.3e-06	39.19	Probable choline kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CK1 PE=2 SV=1	[more]
P20485	3.8e-06	37.18	Choline kinase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55929...	[more]
Q869T9	8.5e-06	26.09	Probable ethanolamine kinase A OS=Dictyostelium discoideum OX=44689 GN=etnkA PE=...	[more]

BLAST of Lsi08G002560 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J1G4	2.1e-63	81.33	probable ethanolamine kinase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481...	[more]
A0A6J1J3G7	2.1e-63	81.33	probable ethanolamine kinase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481...	[more]
A0A5D3D4J8	3.5e-63	81.93	Putative ethanolamine kinase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3CIA0	3.5e-63	81.93	probable ethanolamine kinase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501288 ...	[more]
A0A0A0KDN9	1.0e-62	81.33	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G018610 PE=4 SV=1	[more]

BLAST of Lsi08G002560 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886169.1	1.4e-66	83.13	probable ethanolamine kinase [Benincasa hispida]	[more]
XP_022982034.1	4.3e-63	81.33	probable ethanolamine kinase isoform X1 [Cucurbita maxima]	[more]
XP_023521006.1	4.3e-63	81.33	probable ethanolamine kinase isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022982035.1	4.3e-63	81.33	probable ethanolamine kinase isoform X2 [Cucurbita maxima]	[more]
KAG6607620.1	4.3e-63	81.33	putative ethanolamine kinase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi08G002560 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G26830.1	1.1e-45	66.44	Protein kinase superfamily protein	[more]
AT1G74320.1	4.2e-08	29.57	Protein kinase superfamily protein	[more]
AT1G71697.1	9.4e-08	39.19	choline kinase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.1200.10		coord: 117..174 e-value: 7.5E-24 score: 86.6
None	No IPR available	PFAM	PF01633	Choline_kinase	coord: 110..170 e-value: 8.9E-15 score: 54.9
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 53..157 e-value: 7.5E-24 score: 86.6
None	No IPR available	PANTHER	PTHR22603	CHOLINE/ETHANOALAMINE KINASE	coord: 26..171
None	No IPR available	PANTHER	PTHR22603:SF66	ETHANOLAMINE KINASE 1	coord: 26..171
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 28..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G002560.1	Lsi08G002560.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006646	phosphatidylethanolamine biosynthetic process
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004305	ethanolamine kinase activity