Lsi08G001920 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi08G001920
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Peptide chain release factor 1
Location	chr08: 4045145 .. 4047279 (-)
RNA-Seq Expression	Lsi08G001920
Synteny	Lsi08G001920

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACCGCAAAAATGCTGTGGAATTCCGCTAAGATTCTTGGATTTCCTGTTCCGATAGCAAGCAACCGTAGATGGGCAAGGTCTGGTTGTACATTTCGATTTCGGCATCATCCATTCGAGGGCTCTTTCTCCTTCAACTCGTTGCGTTCTAATTGCAGCTCTAGTAGTGCAAATTCAGTATGGAATGGCAAAGAGTACTTAGTTTTGTCCGACGAAGAGCTGATGAGACAGTGCGAAATGGACACGTTCAAGGCGTCAGGTCCCGGTGGTCAGCACCGCAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCACTGCTCAGGTTTATATTTGTTTCATACATATAATCAATTTCATTAATATACTTCTGTTTCTTTTGCTCTGAAGTTAAATTCTTTGGCCCTTTATTGCCAAGGCTGTCGAGGATCGATCGCAGCATAAGAATCGAGCGAGTGCCTTAACTCGCCTACGTGCTCTCTTGGCCCTAAAAGGTGCGGTGGATTATTACTTTAGATGCTTGTTTTTTGTATCTCATTTCCCAAAATGTTTGTCCTCCTGCTGAATGATATACGATATGTGTTGCTGTATTGAGCTCAGTCAGGAACCCTGTGGATCTTGAATATTATTCCCCTCCTCCAGAGCTTCTTCAAATTCTCCCTCTTAAGTCCACTATTAGGCCACCGGAATGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGTATCTCTCTTTTCTAGTTCTATCTATATGTCTACGTGTACATATGAGTGGGCGTTTATGATTTGATTTTCTTGATATATAAGAAGATTATGAAGGTGATAGCCGAATTACCCACTTTGCAGGGCATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTATCAGACACGGCTAAGCTTTTAGGGTATGTATGCTGTTCATGTGTTACCCTTTGTTTCTTTTTACTTTATTTCCAACATAACTAAGCATCCACAGTTTAACGTAGAGAATGGGAGTGTTTCAATTCTGTGAGAAGATTGGGCTTTTAGCAAGAAATTGAGAGTGCTGATGTGCGGATGTTAATGGTTGATAGGAAGACTAGTGGAAGGGGTATCATTACTAGGTTTATAGTTTTTGTAGCAATGAACAAGAATGGTAATTTTGTCAAGGGCATGAGGGAAGCGAGTTTGGACCTCTGGAAGATGTTGGAGGATAATTTTCTTGGGAAGTGTCTAGCTGGAGGCAAAAGCCTTGCCGTGTCTTGAAGAAAGCTTCTCTCGTAGGATCACTATGAGGAGGAAGGAGCTTTTCCATTTCTTGTATTACTTCTGAGGATGGTATCACTTTTGTTAAGAAGAGTAGGCTTAAGGATTTGATCAGGCAACTTACTACGGAAGCGGACCAAATTTGGTTAGAAAGAGCTTCTTAGTTTGATGACTAGATCTGTGGAAGGACATTTCTTTTGAGCTCCCTTCCTTCTCCCATCTGGTCTGCTGTGGAGTGGGGGAAGGTAAGGAATTGTATTTTTGGGAAGTTCAGTGGGTGGGGGTTAGACCCTTCTGTTTTTCGTGTGCTTGTTTATACCATTAGTTTTTCCTTGAAAATCGTTTGGTTATTGATGTTTTGGTTTGGTTAGGGAGCTTTGTTCTTTCTATTTTGGTTTTGCGATTTTTGTTCTAGAGGGAAGCGATAGAGATTGTTTCTCTCTCTTGTAATTAGATAGGGAGCAGTTTTTGGCTTGTGAGAAAAGATGTTCCTTCATTAAAATATTAACCTAGTGTTTTCCACTATATCTCAAAATGAGATAAACCTGTGACATTATTATTATAAATACTAAAGTAATAAAACGTATGAGACATTAAATATTGAGAGTTTGATGAATTGATGCATTTGGAACTACTGAATTACAATTTAGATAAATAGTCCAGAAATGAGTCTTCAGGTTTCCCATTTTTAATATGTTTAGTGATATAGATTAGTTGCATCCTCTAATAATTATTTCAATGTTAAAAAATTTAGGTTAACCACTGGTGCTCTTTCAAGGTTAATCCTATCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGTAACACCATACTATTTTTGA

mRNA sequence

ATGGCAACCGCAAAAATGCTGTGGAATTCCGCTAAGATTCTTGGATTTCCTGTTCCGATAGCAAGCAACCGTAGATGGGCAAGGTCTGGTTGTACATTTCGATTTCGGCATCATCCATTCGAGGGCTCTTTCTCCTTCAACTCGTTGCGTTCTAATTGCAGCTCTAGTAGTGCAAATTCAGTATGGAATGGCAAAGAGTACTTAGTTTTGTCCGACGAAGAGCTGATGAGACAGTGCGAAATGGACACGTTCAAGGCGTCAGGTCCCGGTGGTCAGCACCGCAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCACTGCTCAGGCTGTCGAGGATCGATCGCAGCATAAGAATCGAGCGAGTGCCTTAACTCGCCTACGTGCTCTCTTGGCCCTAAAAGTCAGGAACCCTGTGGATCTTGAATATTATTCCCCTCCTCCAGAGCTTCTTCAAATTCTCCCTCTTAAGTCCACTATTAGGCCACCGGAATGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGGCATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTATCAGACACGGCTAAGCTTTTAGGGAAGACTAGTGGAAGGGGTATCATTACTAGGTTTATAGTTTTTGTAGCAATGAACAAGAATGGTAATTTTGTCAAGGGCATGAGGGAAGCGAGTTTGGACCTCTGGAAGATGTTAACCACTGGTGCTCTTTCAAGGTTAATCCTATCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGTAACACCATACTATTTTTGA

Coding sequence (CDS)

ATGGCAACCGCAAAAATGCTGTGGAATTCCGCTAAGATTCTTGGATTTCCTGTTCCGATAGCAAGCAACCGTAGATGGGCAAGGTCTGGTTGTACATTTCGATTTCGGCATCATCCATTCGAGGGCTCTTTCTCCTTCAACTCGTTGCGTTCTAATTGCAGCTCTAGTAGTGCAAATTCAGTATGGAATGGCAAAGAGTACTTAGTTTTGTCCGACGAAGAGCTGATGAGACAGTGCGAAATGGACACGTTCAAGGCGTCAGGTCCCGGTGGTCAGCACCGCAACAAGCGCGAGTCTGCTGTTCGTTTGAAGCACATTCCCACTGGTATCACTGCTCAGGCTGTCGAGGATCGATCGCAGCATAAGAATCGAGCGAGTGCCTTAACTCGCCTACGTGCTCTCTTGGCCCTAAAAGTCAGGAACCCTGTGGATCTTGAATATTATTCCCCTCCTCCAGAGCTTCTTCAAATTCTCCCTCTTAAGTCCACTATTAGGCCACCGGAATGTGGCCCTCAAATTGGTCCTAACAATTCTAAGTTTCTGCCGGGCATGCAAGCTCTACTGGATCTAATTTCCACACTTGATGGTTCTGTATCAGACACGGCTAAGCTTTTAGGGAAGACTAGTGGAAGGGGTATCATTACTAGGTTTATAGTTTTTGTAGCAATGAACAAGAATGGTAATTTTGTCAAGGGCATGAGGGAAGCGAGTTTGGACCTCTGGAAGATGTTAACCACTGGTGCTCTTTCAAGGTTAATCCTATCCGATGATGCTCTTAGAATGGCTGTAAATGATATCAGGATGTCCAAGGTAACACCATACTATTTTTGA

Protein sequence

MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGSFSFNSLRSNCSSSSANSVWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSKFLPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLDLWKMLTTGALSRLILSDDALRMAVNDIRMSKVTPYYF

Homology

[Close]

BLAST of Lsi08G001920 vs. ExPASy Swiss-Prot
Match: A7ZE19 (Peptide chain release factor 2 OS=Campylobacter concisus (strain 13826) OX=360104 GN=prfB PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.4e-12
Identity = 34/59 (57.63%), Postives = 45/59 (76.27%), Query Frame = 0


Query: 77  RQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALTRLRALL 136
           +  ++DT++ASG GGQH NK ESA+R+ HIPTGI  Q   DRSQHKNRA+A+  L++ L
Sbjct: 236 KDLKIDTYRASGAGGQHVNKTESAIRITHIPTGIVVQCQNDRSQHKNRATAMKMLKSRL 294

[Close]

BLAST of Lsi08G001920 vs. ExPASy Swiss-Prot
Match: A6Q582 (Peptide chain release factor 2 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 GN=prfB PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.8e-12
Identity = 35/68 (51.47%), Postives = 50/68 (73.53%), Query Frame = 0


Query: 68  LVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASA 127
           +V+ D+++     +DT++ASG GGQH NK +SA+R+ HIPTGI  Q   DRSQHKNRA+A
Sbjct: 231 IVIEDKDI----RVDTYRASGAGGQHVNKTDSAIRITHIPTGIVVQCQNDRSQHKNRATA 290

Query: 128 LTRLRALL 136
           +  L++ L
Sbjct: 291 MKMLKSRL 294

[Close]

BLAST of Lsi08G001920 vs. ExPASy Swiss-Prot
Match: B2U8V1 (Peptide chain release factor 2 OS=Ralstonia pickettii (strain 12J) OX=402626 GN=prfB PE=3 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.4e-12
Identity = 34/55 (61.82%), Postives = 43/55 (78.18%), Query Frame = 0


Query: 81  MDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALTRLRALL 136
           +DT++ASG GGQH NK +SAVR+ HIPTGI  Q   DRSQH+NRA A+T L++ L
Sbjct: 243 VDTYRASGAGGQHINKTDSAVRITHIPTGIVVQCQNDRSQHRNRAEAMTMLKSRL 297

[Close]

BLAST of Lsi08G001920 vs. ExPASy Swiss-Prot
Match: Q47Y94 (Peptide chain release factor 1 OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) OX=167879 GN=prfA PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.3e-12
Identity = 33/56 (58.93%), Postives = 44/56 (78.57%), Query Frame = 0


Query: 80  EMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALTRLRALL 136
           ++DTF+ASG GGQH NK +SA+R+ HIPTG+  +  E RSQHKNRA A++ L+A L
Sbjct: 225 KVDTFRASGAGGQHVNKTDSAIRITHIPTGVVVECQEQRSQHKNRAQAMSVLQARL 280

[Close]

BLAST of Lsi08G001920 vs. ExPASy Swiss-Prot
Match: Q12F86 (Peptide chain release factor 1 OS=Polaromonas sp. (strain JS666 / ATCC BAA-500) OX=296591 GN=prfA PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 5.3e-12
Identity = 35/61 (57.38%), Postives = 46/61 (75.41%), Query Frame = 0


Query: 81  MDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALTRLRALLALKVR 140
           +DT++ASG GGQH NK +SAVR+ HIPTGI A+  +DRSQH+N+A AL  L A +  K R
Sbjct: 234 IDTYRASGAGGQHINKTDSAVRITHIPTGIVAECQDDRSQHRNKAKALQVLSARIVEKDR 293

Query: 141 N 142
           +
Sbjct: 294 S 294

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BLAST of Lsi08G001920 vs. ExPASy TrEMBL
Match: A0A5A7T8S5 (Peptide chain release factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001580 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 2.2e-106
Identity = 213/271 (78.60%), Postives = 218/271 (80.44%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGS-FSFNSLRSNCSSSSAN 60
           MA A MLWNSAKILGFP PIASN RWAR G  FRFRHHPFEGS F FNSLR NCSSS+AN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           SV +GKEYLVLSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SAL RLRALLALKVRNPVDLE YSPPPELLQILP KST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLD 240
           FLPGMQALLDLISTLDGSVSDTAKLLG                                 
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLG--------------------------------- 234

Query: 241 LWKMLTTGALSRLILSDDALRMAVNDIRMSK 271
               LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ----LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of Lsi08G001920 vs. ExPASy TrEMBL
Match: A0A1S3CAA2 (uncharacterized protein LOC103498609 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498609 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 2.2e-106
Identity = 213/271 (78.60%), Postives = 218/271 (80.44%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGS-FSFNSLRSNCSSSSAN 60
           MA A MLWNSAKILGFP PIASN RWAR G  FRFRHHPFEGS F FNSLR NCSSS+AN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           SV +GKEYLVLSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SAL RLRALLALKVRNPVDLE YSPPPELLQILP KST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLD 240
           FLPGMQALLDLISTLDGSVSDTAKLLG                                 
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLG--------------------------------- 234

Query: 241 LWKMLTTGALSRLILSDDALRMAVNDIRMSK 271
               LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ----LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of Lsi08G001920 vs. ExPASy TrEMBL
Match: A0A0A0KUM8 (RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G646680 PE=4 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 7.2e-105
Identity = 211/271 (77.86%), Postives = 217/271 (80.07%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGS-FSFNSLRSNCSSSSAN 60
           MA A MLW+SAKILGFP PIASN RWAR   TFR RH PFEGS F FNSLR NCSSS+AN
Sbjct: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           SV NGK+YLVLSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SAL RLRALLALKVRNPVDLE YSPPPELLQILPLKST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLD 240
           FLPGMQALLDLISTLDGSVSDTAKLLG                                 
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLG--------------------------------- 234

Query: 241 LWKMLTTGALSRLILSDDALRMAVNDIRMSK 271
               LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ----LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of Lsi08G001920 vs. ExPASy TrEMBL
Match: A0A6J1EX22 (uncharacterized protein LOC111438876 OS=Cucurbita moschata OX=3662 GN=LOC111438876 PE=4 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 2.3e-103
Identity = 206/270 (76.30%), Postives = 212/270 (78.52%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGSFSFNSLRSNCSSSSANS 60
           MATA MLW SAKIL  PVPIASN RW R GCTFR   H FEGSF  NSLRS CSSSSANS
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRI--HQFEGSFFSNSLRSTCSSSSANS 60

Query: 61  VWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120
           VW GKEYL+LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ
Sbjct: 61  VWKGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120

Query: 121 HKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSKF 180
           HKNRASAL RLRA LALKVRNPVDLE YSPPPELLQILPLKSTIRPP CGPQIGPNNSKF
Sbjct: 121 HKNRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKF 180

Query: 181 LPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLDL 240
           LPGMQALLDLI TLDGS+SDTAK +G                                  
Sbjct: 181 LPGMQALLDLIYTLDGSISDTAKYIG---------------------------------- 231

Query: 241 WKMLTTGALSRLILSDDALRMAVNDIRMSK 271
              LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ---LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of Lsi08G001920 vs. ExPASy TrEMBL
Match: A0A6J1I8P1 (uncharacterized protein LOC111471993 OS=Cucurbita maxima OX=3661 GN=LOC111471993 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.3e-101
Identity = 203/270 (75.19%), Postives = 211/270 (78.15%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGSFSFNSLRSNCSSSSANS 60
           MAT  MLW SAKIL  PVPIASN RW R GCTFR   H FEGSF  NSLRSNCSSSSA S
Sbjct: 1   MATTNMLWKSAKILSIPVPIASNGRWGRIGCTFRI--HQFEGSFFSNSLRSNCSSSSAIS 60

Query: 61  VWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120
           VW GKEYL+LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ
Sbjct: 61  VWKGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120

Query: 121 HKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSKF 180
           HKNRASAL RLRA LALKVRNPV+LE YSPPPELLQILPLKSTIRPP CGPQIGPNNSKF
Sbjct: 121 HKNRASALARLRAQLALKVRNPVNLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKF 180

Query: 181 LPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLDL 240
           LPGMQ+LLDLI TLDGS+SDTAK +G                                  
Sbjct: 181 LPGMQSLLDLIYTLDGSISDTAKYIG---------------------------------- 231

Query: 241 WKMLTTGALSRLILSDDALRMAVNDIRMSK 271
              LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ---LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of Lsi08G001920 vs. NCBI nr
Match: XP_038891024.1 (peptide chain release factor 1 [Benincasa hispida])

HSP 1 Score: 397.9 bits (1021), Expect = 7.1e-107
Identity = 213/270 (78.89%), Postives = 218/270 (80.74%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGSFSFNSLRSNCSSSSANS 60
           MA A ML NSAKILGFP+PIAS  RWAR GCT  F HHPFEGSF FNSL SNCSS++ANS
Sbjct: 1   MANANMLCNSAKILGFPLPIASKGRWARFGCT--FWHHPFEGSFFFNSLHSNCSSTTANS 60

Query: 61  VWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120
           VWNGKEYLVLSDEELM+QCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ
Sbjct: 61  VWNGKEYLVLSDEELMKQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120

Query: 121 HKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSKF 180
           HKNRASAL RLRA LALKVRNPVDLE YSPPPELLQILPLKSTIRPPECGPQIGP NSKF
Sbjct: 121 HKNRASALARLRAFLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPECGPQIGPKNSKF 180

Query: 181 LPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLDL 240
           LPGMQALLDLISTLDGSVSDTAKLLG                                  
Sbjct: 181 LPGMQALLDLISTLDGSVSDTAKLLG---------------------------------- 231

Query: 241 WKMLTTGALSRLILSDDALRMAVNDIRMSK 271
              LTTGALSRLILSDDALRMAVNDIR+SK
Sbjct: 241 ---LTTGALSRLILSDDALRMAVNDIRISK 231

[Close]

BLAST of Lsi08G001920 vs. NCBI nr
Match: XP_008459491.1 (PREDICTED: uncharacterized protein LOC103498609 isoform X1 [Cucumis melo] >KAA0039363.1 peptide chain release factor 1 [Cucumis melo var. makuwa] >TYK00546.1 peptide chain release factor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 395.2 bits (1014), Expect = 4.6e-106
Identity = 213/271 (78.60%), Postives = 218/271 (80.44%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGS-FSFNSLRSNCSSSSAN 60
           MA A MLWNSAKILGFP PIASN RWAR G  FRFRHHPFEGS F FNSLR NCSSS+AN
Sbjct: 1   MANANMLWNSAKILGFPAPIASNVRWARFGYKFRFRHHPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           SV +GKEYLVLSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCSGKEYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SAL RLRALLALKVRNPVDLE YSPPPELLQILP KST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPPKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLD 240
           FLPGMQALLDLISTLDGSVSDTAKLLG                                 
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLG--------------------------------- 234

Query: 241 LWKMLTTGALSRLILSDDALRMAVNDIRMSK 271
               LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ----LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of Lsi08G001920 vs. NCBI nr
Match: XP_004141513.1 (uncharacterized protein LOC101217430 isoform X1 [Cucumis sativus] >KGN52594.1 hypothetical protein Csa_007986 [Cucumis sativus])

HSP 1 Score: 390.2 bits (1001), Expect = 1.5e-104
Identity = 211/271 (77.86%), Postives = 217/271 (80.07%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGS-FSFNSLRSNCSSSSAN 60
           MA A MLW+SAKILGFP PIASN RWAR   TFR RH PFEGS F FNSLR NCSSS+AN
Sbjct: 1   MANANMLWSSAKILGFPSPIASNVRWARFDYTFRLRHRPFEGSFFFFNSLRFNCSSSNAN 60

Query: 61  SVWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRS 120
           SV NGK+YLVLSDEELM+QCEM TFKASGPGGQHRNKRESAVRLKHIPTGI AQAVEDRS
Sbjct: 61  SVCNGKQYLVLSDEELMKQCEMGTFKASGPGGQHRNKRESAVRLKHIPTGIIAQAVEDRS 120

Query: 121 QHKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSK 180
           QHKNR+SAL RLRALLALKVRNPVDLE YSPPPELLQILPLKST+RPPECGPQIGPNNSK
Sbjct: 121 QHKNRSSALARLRALLALKVRNPVDLEDYSPPPELLQILPLKSTVRPPECGPQIGPNNSK 180

Query: 181 FLPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLD 240
           FLPGMQALLDLISTLDGSVSDTAKLLG                                 
Sbjct: 181 FLPGMQALLDLISTLDGSVSDTAKLLG--------------------------------- 234

Query: 241 LWKMLTTGALSRLILSDDALRMAVNDIRMSK 271
               LTTGALSRLILSDDALRMAVNDIRMSK
Sbjct: 241 ----LTTGALSRLILSDDALRMAVNDIRMSK 234

[Close]

BLAST of Lsi08G001920 vs. NCBI nr
Match: XP_023523846.1 (uncharacterized protein LOC111787964 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 387.9 bits (995), Expect = 7.4e-104
Identity = 208/270 (77.04%), Postives = 213/270 (78.89%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGSFSFNSLRSNCSSSSANS 60
           MATA MLW SAKIL  PVPIASN RW R GCTFR   H FEGSF  NSLRSNCSSSSANS
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRI--HQFEGSFFSNSLRSNCSSSSANS 60

Query: 61  VWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120
           VW GKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ
Sbjct: 61  VWKGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120

Query: 121 HKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSKF 180
           HKNRASAL RLRA LALKVRNPVDLE YSPPPELLQILPLKSTIRPP CGPQIGPNNSKF
Sbjct: 121 HKNRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKF 180

Query: 181 LPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLDL 240
           LPGMQALLDLI TLDGS+SDTAK +G                                  
Sbjct: 181 LPGMQALLDLIYTLDGSISDTAKYIG---------------------------------- 231

Query: 241 WKMLTTGALSRLILSDDALRMAVNDIRMSK 271
              LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ---LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of Lsi08G001920 vs. NCBI nr
Match: KAG6607552.1 (hypothetical protein SDJN03_00894, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037191.1 prfB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 387.1 bits (993), Expect = 1.3e-103
Identity = 206/270 (76.30%), Postives = 213/270 (78.89%), Query Frame = 0


Query: 1   MATAKMLWNSAKILGFPVPIASNRRWARSGCTFRFRHHPFEGSFSFNSLRSNCSSSSANS 60
           MATA MLW SAKIL  PVPIASN RW R GCTFR   H FEGSF  NSLRSNCSSSSANS
Sbjct: 1   MATANMLWKSAKILSIPVPIASNGRWGRIGCTFRI--HQFEGSFFSNSLRSNCSSSSANS 60

Query: 61  VWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120
           +W GKEYL+LSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ
Sbjct: 61  IWKGKEYLILSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQ 120

Query: 121 HKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLKSTIRPPECGPQIGPNNSKF 180
           HKNRASAL RLRA LALKVRNPVDLE YSPPPELLQILPLKSTIRPP CGPQIGPNNSKF
Sbjct: 121 HKNRASALARLRAQLALKVRNPVDLEDYSPPPELLQILPLKSTIRPPGCGPQIGPNNSKF 180

Query: 181 LPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFVAMNKNGNFVKGMREASLDL 240
           LPGMQALLDLI TLDGS+SDTAK +G                                  
Sbjct: 181 LPGMQALLDLIYTLDGSISDTAKYIG---------------------------------- 231

Query: 241 WKMLTTGALSRLILSDDALRMAVNDIRMSK 271
              LTTGA+SRLILSDD+LRMAVNDIR+SK
Sbjct: 241 ---LTTGAVSRLILSDDSLRMAVNDIRISK 231

[Close]

BLAST of Lsi08G001920 vs. TAIR 10
Match: AT1G33330.1 (Class I peptide chain release factor )

HSP 1 Score: 231.1 bits (588), Expect = 1.1e-60
Identity = 131/229 (57.21%), Postives = 151/229 (65.94%), Query Frame = 0


Query: 42  GSFSFNSLRSNCSSSSANSVWNGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAV 101
           G  S     ++  S S  S      YL  +DEELM+QC ++TF+ SGPGGQHRNKR+SAV
Sbjct: 61  GGSSGGDFGNDNDSVSVVSDVQSPNYLKFTDEELMKQCRLETFRVSGPGGQHRNKRDSAV 120

Query: 102 RLKHIPTGITAQAVEDRSQHKNRASALTRLRALLALKVRNPVDLEYYSPPPELLQILPLK 161
           RLKH+PTGI AQAVEDRSQHKNRASAL RLR LLA+KVRN VD+E Y+PPPELLQILP K
Sbjct: 121 RLKHLPTGIVAQAVEDRSQHKNRASALNRLRTLLAIKVRNKVDIEAYAPPPELLQILPPK 180

Query: 162 STIRPPECGPQIGPNNSKFLPGMQALLDLISTLDGSVSDTAKLLGKTSGRGIITRFIVFV 221
           STIR    G QIGPNN KF+PGMQALLD+IS  DGS++D+AKLLG               
Sbjct: 181 STIR-TSSGSQIGPNNPKFVPGMQALLDVISASDGSIADSAKLLG--------------- 240

Query: 222 AMNKNGNFVKGMREASLDLWKMLTTGALSRLILSDDALRMAVNDIRMSK 271
                                 L+TG LSRLILS D LRMAVN +R +K
Sbjct: 241 ----------------------LSTGGLSRLILSHDGLRMAVNSMRAAK 251

[Close]

BLAST of Lsi08G001920 vs. TAIR 10
Match: AT1G56350.1 (Peptide chain release factor 2 )

HSP 1 Score: 64.3 bits (155), Expect = 1.7e-10
Identity = 32/73 (43.84%), Postives = 48/73 (65.75%), Query Frame = 0


Query: 63  NGKEYLVLSDEELMRQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHK 122
           +G   + ++D +L     ++ F++ G GGQH N  +SAVR+ HIPTGITA    +RSQH 
Sbjct: 336 DGSTRVEINDSDL----RIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHS 395

Query: 123 NRASALTRLRALL 136
           N+ASA+  L++ L
Sbjct: 396 NKASAMAVLQSRL 404

[Close]

BLAST of Lsi08G001920 vs. TAIR 10
Match: AT2G47020.1 (Peptide chain release factor 1 )

HSP 1 Score: 58.9 bits (141), Expect = 7.3e-09
Identity = 28/55 (50.91%), Postives = 37/55 (67.27%), Query Frame = 0


Query: 81  MDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAVEDRSQHKNRASALTRLRALL 136
           +DT+++ G GGQH N   SAVR+ H+PTG+     ++RSQH NRA AL  L A L
Sbjct: 276 IDTYRSGGSGGQHANTTNSAVRIIHLPTGMMVSIQDERSQHMNRAKALKVLCARL 330

[Close]

BLAST of Lsi08G001920 vs. TAIR 10
Match: AT5G36170.1 (high chlorophyll fluorescent 109 )

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-08
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 0


Query: 62  WNGKEYLVLSDEELM------RQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAV 121
           ++G E + L  EE +         ++   +A G GGQ+ NK E+AVR+ HIPTG+  +  
Sbjct: 296 FSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCT 355

Query: 122 EDRSQHKNRASALTRLRALL 136
           E+RSQ  N+  AL RL+A L
Sbjct: 356 EERSQLANKTRALIRLKAKL 375

[Close]

BLAST of Lsi08G001920 vs. TAIR 10
Match: AT5G36170.2 (high chlorophyll fluorescent 109 )

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-08
Identity = 32/80 (40.00%), Postives = 47/80 (58.75%), Query Frame = 0


Query: 62  WNGKEYLVLSDEELM------RQCEMDTFKASGPGGQHRNKRESAVRLKHIPTGITAQAV 121
           ++G E + L  EE +         ++   +A G GGQ+ NK E+AVR+ HIPTG+  +  
Sbjct: 295 FSGVEVMPLLPEEAVGIEIPEEDLDISFTRAGGKGGQNVNKVETAVRITHIPTGVAVRCT 354

Query: 122 EDRSQHKNRASALTRLRALL 136
           E+RSQ  N+  AL RL+A L
Sbjct: 355 EERSQLANKTRALIRLKAKL 374

The following BLAST results are available for this feature:

BLAST of Lsi08G001920 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7ZE19	1.4e-12	57.63	Peptide chain release factor 2 OS=Campylobacter concisus (strain 13826) OX=36010...	[more]
A6Q582	1.8e-12	51.47	Peptide chain release factor 2 OS=Nitratiruptor sp. (strain SB155-2) OX=387092 G...	[more]
B2U8V1	2.4e-12	61.82	Peptide chain release factor 2 OS=Ralstonia pickettii (strain 12J) OX=402626 GN=...	[more]
Q47Y94	5.3e-12	58.93	Peptide chain release factor 1 OS=Colwellia psychrerythraea (strain 34H / ATCC B...	[more]
Q12F86	5.3e-12	57.38	Peptide chain release factor 1 OS=Polaromonas sp. (strain JS666 / ATCC BAA-500) ...	[more]

BLAST of Lsi08G001920 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7T8S5	2.2e-106	78.60	Peptide chain release factor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3CAA2	2.2e-106	78.60	uncharacterized protein LOC103498609 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0KUM8	7.2e-105	77.86	RF_PROK_I domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G646680 P...	[more]
A0A6J1EX22	2.3e-103	76.30	uncharacterized protein LOC111438876 OS=Cucurbita moschata OX=3662 GN=LOC1114388...	[more]
A0A6J1I8P1	1.3e-101	75.19	uncharacterized protein LOC111471993 OS=Cucurbita maxima OX=3661 GN=LOC111471993...	[more]

BLAST of Lsi08G001920 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891024.1	7.1e-107	78.89	peptide chain release factor 1 [Benincasa hispida]	[more]
XP_008459491.1	4.6e-106	78.60	PREDICTED: uncharacterized protein LOC103498609 isoform X1 [Cucumis melo] >KAA00...	[more]
XP_004141513.1	1.5e-104	77.86	uncharacterized protein LOC101217430 isoform X1 [Cucumis sativus] >KGN52594.1 hy...	[more]
XP_023523846.1	7.4e-104	77.04	uncharacterized protein LOC111787964 [Cucurbita pepo subsp. pepo]	[more]
KAG6607552.1	1.3e-103	76.30	hypothetical protein SDJN03_00894, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lsi08G001920 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G33330.1	1.1e-60	57.21	Class I peptide chain release factor	[more]
AT1G56350.1	1.7e-10	43.84	Peptide chain release factor 2	[more]
AT2G47020.1	7.3e-09	50.91	Peptide chain release factor 1	[more]
AT5G36170.1	2.1e-08	40.00	high chlorophyll fluorescent 109	[more]
AT5G36170.2	2.1e-08	40.00	high chlorophyll fluorescent 109	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 78..136 e-value: 5.5E-20 score: 71.5
None	No IPR available	GENE3D	3.30.160.20		coord: 36..142 e-value: 6.1E-26 score: 92.3
None	No IPR available	PANTHER	PTHR43804	LD18447P	coord: 43..272
None	No IPR available	PANTHER	PTHR43804:SF6	CLASS I PEPTIDE CHAIN RELEASE FACTOR	coord: 43..272
None	No IPR available	SUPERFAMILY	75620	Release factor	coord: 77..140

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi08G001920.1	Lsi08G001920.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006415	translational termination
molecular_function	GO:0003747	translation release factor activity