Lsi08G001610 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G001610 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 6.3e-38
Identity = 168/666 (25.23%), Postives = 282/666 (42.34%), Query Frame = 0

Query: 59  PLPTNSFFQNFVLNNGDLPEYIHPYLVRTAN----YSISVSY---PSRISNSSITLLP-- 118
           P+ TN F+ N  +     P + HPY V   N    Y +++S+     R+       +P  
Sbjct: 34  PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNGSSYYGLAISHIDDSQRVFGPDPESVPCQ 93

Query: 119 -FFS-----DLTISALNRTS----PVSNTTHFISSFSDLGVDLDIGDFRFHLVRG----- 178
            +F+      + ISA    S     +  + HF    +        G     +V G     
Sbjct: 94  YYFNPAGLYSIIISAREFASGNLLSLDQSRHFSIQATLSATTSGSGTIILPIVAGMGFVS 153

Query: 179 ------SPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLSNGRNWVLY-----SS 238
                 +P    S+L +S   I    G           K+ I+L++G+ W LY     SS
Sbjct: 154 GYYTNLTPVFNSSILFSSITKINFSRGY----------KYRIQLTDGKIWFLYAFPTSSS 213

Query: 239 SAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMES---EKILDRYSGCYPVSGFVKLSEN 298
           S   L  + N+Q+ TS  F+G+I++  +PN ++ +   + I D  +G Y  S  +    +
Sbjct: 214 STFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIYDASAGVYTTSISLSAQVS 273

Query: 299 FGFKYKWQKKGSGGL-----LMLAHPLHRQIL-SGNQTVLQNLRYGSID-GDLLGVVGDS 358
                 W +  + G      LM A P H Q   S  Q     L   S   G +      +
Sbjct: 274 GTTGEYWFRFATAGYTNLNPLMFALPHHMQSFGSDTQAYKTGLGLASTTMGIMFAYATKT 333

Query: 359 WDL---------NFHPIPITWHSIDGIDRKFFPEIIAALKRDVAT---LNVAELSSTAAS 418
           W L          F PIP  W   +G    + P  +AA++   AT    +V   S+  + 
Sbjct: 334 WHLIEKNLPTQVGFLPIP--W---NGGSNTYSPTALAAIRAACATDINFDVVNASNLDSM 393

Query: 419 YFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKN----GFLYERK 478
           Y  GK++A  A++ L+A  +   + +    +  LK       + +F  N      +Y+  
Sbjct: 394 YTSGKIVAMYAQVCLVASRILGDSTLTNTGLTKLKQA-----MARFTTNTQMYPLVYDTT 453

Query: 479 WSGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQ-AYALVN 538
           + G+++  G +S   D+G   YNDHHFH GY +Y+ AV+  LDP+W      +   AL+ 
Sbjct: 454 YKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIGLLDPSWLVNDNIRYVNALLR 513

Query: 539 DYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAY 598
           D  N     + F++ FRNFD++  HSWA G+ E   G+++ESTSE  N  YA  L G+  
Sbjct: 514 DSANPSESDTYFAM-FRNFDWFVGHSWATGIFESGDGKDEESTSEDFNFLYATKLWGMVR 573

Query: 599 DDESLTAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFA 658
           +D  L    + + A    +  T+ ++    +          N V GI +    +   +F+
Sbjct: 574 NDTVLINRANLMLAVLKNSLNTYIYMTPTTS--VQPSQILGNYVTGITFMNKVDYATYFS 633

Query: 659 PAEWRECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGI 663
             E+  C+ GI ++P  P++  + S     ++    ++P +        W G  ++   I
Sbjct: 634 AEEY--CKQGIHMIPTTPISGYLRSPSYVQQDWNAKIAPII--NTFSNNWTGILWSNYAI 672

BLAST of Lsi08G001610 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 1.1e-37
Identity = 162/545 (29.72%), Postives = 250/545 (45.87%), Query Frame = 0

Query: 149 FHLVRGSPYLT--FSVLKTSSVSITTGNGVRSVDSYEDST-KHIIRLSNGRNWVLY---S 208
           F LV+G  ++T  ++ L+ +  S      V    S +    K+ I L + +NW+LY    
Sbjct: 340 FPLVQGMGFITAIYNNLQPAIQSAVLFRKVEPAGSPQGGIFKYKITLEDDKNWLLYVIPE 399

Query: 209 SSAIYLIKSKNNQIVTSGV-FSGVIRVAVLPNSAMESEKILDRYSGCY----PVSGFVKL 268
           + A   +K + N++++    F GVI+VA  P SA E E I D+ +G Y     +SG V  
Sbjct: 400 NGADPKLKLEGNKLISGPTGFKGVIQVAKNP-SAEEGEGIYDKSAGSYATDIKISGSVGT 459

Query: 269 SENFGFKYKWQKKGSGG-LLMLAHPLHRQIL-SGNQTVLQNLRYG-SIDGDLLGVVGDSW 328
                +K+ ++K G G  L+M A P H +      +   +N++   +  G     VGDSW
Sbjct: 460 DGTGTYKFSFEKAGKGAPLVMYALPHHVESFDDATKNTKKNMKLSTTTKGMATACVGDSW 519

Query: 329 DLNFHPIPIT-----W---HSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGK 388
            +    +P++     W    S      +     I A+  +  + ++   ++  + YF GK
Sbjct: 520 TMVEGNLPLSMDFAPWKPGSSSQVTLSEGAKNAIKAVAGNELSQDMELQTNLNSMYFSGK 579

Query: 389 LLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKN- 448
            L + A      +E+          +  LK   + ++  K  +   +Y+  W G+V+   
Sbjct: 580 GLNKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFVDNK-QQIPLVYDNVWKGVVSSGT 639

Query: 449 -GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPK 508
                T  DFG  +YNDHHFH GYF+ + A+L KLDP W    K     LV D  N    
Sbjct: 640 YEKGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVNMLVRDSGNSVDN 699

Query: 509 KSQFSIPF-RNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESLTA 568
              F  PF R FD++  HSWA GL E   G++QESTSE     YA  + G    D+S+ A
Sbjct: 700 DEHF--PFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMWGKTSGDKSMEA 759

Query: 569 AGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWF-APAEWRE 628
            G+ L    +A T   + + +  N      F   N+V GIL+    +   +F A  E+ +
Sbjct: 760 RGN-LMLGILARTLNNYFLMKNDNVNQPKNFI-GNKVTGILFENKIDHTTYFGANLEYIQ 819

Query: 629 CRVGIQVLPVLPVTERVFSDEGFVKEVVEWVS-----PALEREDAGEGWKGFAYALEGIY 663
              GI +LP+LP +    S E FVKE  EW +      A   E    GWKG  YA   I 
Sbjct: 820 ---GIHMLPLLPNSAYTRSAE-FVKE--EWEAMFASGAAAPAEKVTGGWKGVLYANLAII 872

BLAST of Lsi08G001610 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.2e-36
Identity = 175/670 (26.12%), Postives = 298/670 (44.48%), Query Frame = 0

Query: 59   PLPTNSFFQNFVLNNGDLPEYIHPYLV---RTANYSISVSY------------------- 118
            P+ TN F+ N ++ + + P +++PY +    +++Y  +V +                   
Sbjct: 433  PIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSSSYGFAVQHTTVDQYSYGGYDSSGNAEY 492

Query: 119  ---PSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVR 178
               P  I++   +   F S +T+     T  +S+T   +S  +D    L+I      LV+
Sbjct: 493  LVNPLGIAHVVFSASNFDSSMTMQVDEMT--LSSTRVVLSESNDSSNYLEI-----PLVQ 552

Query: 179  GSPYLTFSVLKTSSVSITTGNGVRSVDSYEDST------KHIIRLSNGRNWVLY------ 238
            G  + T     + +  I +  G  ++ S   S       K+ I L NG  W+ Y      
Sbjct: 553  GMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYRITLLNGVTWLCYVIGPDD 612

Query: 239  -SSSAIYLIKSKNNQIVTSGVFSG-VIRVAVLPNSAMESEKILDRYSGCYPVSGFVKL-- 298
             +S+   L  S   +I  S    G +I++AV P S  + E   D+ +G Y V+ F KL  
Sbjct: 613  LTSTDFSLEVSSEYEIKASASVDGLIIQLAVAP-SETDYEVFYDQAAGMY-VTNF-KLQG 672

Query: 299  -----SENFGFKYKWQ-KKGSGGLLMLAHPLHR----QILSGNQTVLQ--NLRYGSIDGD 358
                 +  + F Y  Q +  SG  ++ A P H      I+    T +Q  +   G ++G 
Sbjct: 673  VSDGSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQDYYTGIQLASTTKGVMNGY 732

Query: 359  LLGVVGDSWDLNFHPIPITWHSIDGID----RKFFPEIIAALKRDVATLNVAELSSTAAS 418
            L   +  S  LN     + W S  G +     K   +++A +      ++++E  S   +
Sbjct: 733  LTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAEVANSELQVSISESISGLNT 792

Query: 419  YFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGL 478
            Y+ GK++ + + + L   E+        + ++ +KS     L+        +Y+ K++GL
Sbjct: 793  YYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFD-ILLQNEQTYPLIYDTKFNGL 852

Query: 479  VTKN--GATSTTEDFGFGIYNDHHFHLGYFVYSIAVL----AKLDPNWGKQFKPQAYALV 538
            V+    G+TST  DFG   YNDHHFH GY +++ AV+    +KL+  W    K    +LV
Sbjct: 853  VSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVDSKLNGTWAADNKDWVNSLV 912

Query: 539  NDYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLA 598
             D  N   +K ++    R FD++  HSWAAGL E   G+N+ES+SE  N  YA  L G  
Sbjct: 913  RDVAN-PSEKDEYFAQSRMFDWFNGHSWAAGLYENGNGKNEESSSEDYNFAYAMKLWGAT 972

Query: 599  YDDESLTAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEE----NRVVGILWSGGRES 658
              D+S+   G  + +    A   +++ +       DN    E    N+V GIL+    + 
Sbjct: 973  IGDQSMELRGDLMISIMKDAMNDYFYYQN------DNTVEPEEIIGNKVSGILFDNIIDY 1032

BLAST of Lsi08G001610 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 6.5e-35
Identity = 165/668 (24.70%), Postives = 286/668 (42.81%), Query Frame = 0

Query: 59  PLPTNSFFQNFVLNNGDLPEYIHPYLV----------RTANYSISVSYPSRISNSSITLL 118
           PL TN F+ N +L++   P + HPY +            AN++++     R+ +++    
Sbjct: 110 PLETNKFYTNMLLDDNTQPIWTHPYSIWFSRDPELFGLAANHTLA---SQRVFDTTTNPP 169

Query: 119 PFFSDLTISALNRTSPVSNTTHFISS------FSD-------LGVDLDIGDF-RFHLVRG 178
            F+ + T    N  S V     F+SS      F D       L + L    F  F LV+G
Sbjct: 170 RFYFNPT----NIKSFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFPLVQG 229

Query: 179 SPYLTFSVLKTSSVSITTGNGVRSVDSY---EDSTKHIIRLSNGRNWVLYSSSAIYLIKS 238
             ++T ++       + +  G RS++     E   K+ I+L N RNW+LY +S  Y    
Sbjct: 230 MGFVT-AIYHDLGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQ 289

Query: 239 -------KNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPV----SGFVKLSEN 298
                   +N I++S   +G+I        + +S   +D  +GCYPV    SG       
Sbjct: 290 DFQISLLDSNTIISSHKINGLI-----CQLSADSVPSIDMAAGCYPVYCDLSGQTVDEHF 349

Query: 299 FGFKYKWQKKG---SGGLLMLAHPLH----------RQILSGNQTVLQNLRYGSIDGDLL 358
             +++ +   G   SG  LM A P H          R+I S   + ++ L  G +     
Sbjct: 350 TNYRFNYTVAGYSQSGTTLMYALPHHKAAFTPEMQEREIASSLDSTVKGLMTGYLTNSFD 409

Query: 359 GVVGDSWDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGKL 418
             V    +L F P+ ++ +      ++   +I  A  ++V   +  + S+  + YF GK+
Sbjct: 410 MQVQVPQELGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGKI 469

Query: 419 LARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGK--FPKNGFLYERKWSGLVTKN 478
           LA+ A +  +   + +   +   ++  L   ++ ++  +   P N   Y+  W G+++  
Sbjct: 470 LAKYAWILYVTHYILHDENLTKELLSKLTIAMERFISNQQVLPLN---YDVSWKGIISSG 529

Query: 479 GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDP--------NWGKQFKPQAYALVND 538
              S+++DFG   YNDHHFH  Y V + A+++ +D         +W +  +     L+ D
Sbjct: 530 ---SSSQDFGNSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRD 589

Query: 539 YMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYD 598
           Y         F   FR+FD++  HSWA GL     G+++ESTSE VN+ YA  L GL   
Sbjct: 590 YSGVDNDDPYFP-QFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTG 649

Query: 599 DESLTAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAP 658
           +  LT   +          Q+++ +    N +    F   N+V GIL+    +   +F  
Sbjct: 650 NSKLTDIANLQLGIMRNVFQSYF-LYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGM 709

Query: 659 AEWRECRVGIQVLPVLPVTERV--FSDEGFVKEVVEW---VSPALEREDAGEGWKGFAYA 661
                    I ++  +P+T          FVKE  EW   + P +++    +GWKG    
Sbjct: 710 EPQY-----IHMIHAIPITSASSWVRTPNFVKE--EWEEKMQPIIDQ--VNDGWKGIIML 746

BLAST of Lsi08G001610 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 5.9e-28
Identity = 121/500 (24.20%), Postives = 219/500 (43.80%), Query Frame = 0

Query: 187 KHIIRLSNGRNWVLY-SSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRY 246
           K+ + +S+   W++Y    ++ L +S +  +V S  F+G I++A +P     +E + D Y
Sbjct: 226 KYRVTMSDNHVWLIYIYGDSLTLTESTSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTY 285

Query: 247 SGCY----PVSGFVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQI---LSGNQTVLQNLR 306
           +G Y     +SG+V+ +  + + + +   G   +  L   L  Q+   +SG + V   + 
Sbjct: 286 AGVYITGISISGYVEDAVGY-YSFDFTTAGDTSVEPLFFLLPHQVDTAVSGTK-VTSIVL 345

Query: 307 YGSIDGDLLGVVGDS--------WDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATL 366
              + GD+    G+S         D+ F P   T   I   +     EIIA +       
Sbjct: 346 ASLVSGDMNAAAGNSITFAEAIPQDIGFLPWSPTGGQIGYSEEAL--EIIAEVAGTELGE 405

Query: 367 NVAELSSTAASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGK--FP 426
           + +  S+  + Y+ GK+LA+ A L +   ++          ++ L++    ++  +  +P
Sbjct: 406 DFSANSNLNSMYYSGKVLAKYAMLCVTINDILGDETSSEQCIQKLEAAFARFVDNQQIYP 465

Query: 427 KNGFLYERKWSGLVTKNGAT-STTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQF 486
                Y+  W G+V+  G +  +  DFG   YNDHHFH GYFV++ AV+  +DP+W    
Sbjct: 466 ---LTYDNTWKGVVSVAGLSGDSLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTG 525

Query: 487 KPQAYA--LVNDYMNFRPKKSQFSIP-FRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVN 546
             + +   LV D  N  P  +    P  R  D +  H WA+GL E   G+++ESTSE  N
Sbjct: 526 NNKEWVNFLVRDVAN--PSSNDPYFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYN 585

Query: 547 AYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGIL 606
            ++   L G    D  +    + +   E  A   +       +G       + N V GI 
Sbjct: 586 FFFGMKLWGQVIGDSDMEDRANIILGIERNALNKY---MLYADGNVQPTSMQPNYVAGIT 645

Query: 607 WSGGRESRLWFAPAEWRECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGE 665
           +        +F      E   GI +LP+ P++  +      + E    ++  ++  D+  
Sbjct: 646 FMNKITHTTYFGTN--IEYIQGIHMLPITPISAFIRGPSFVLAEWNALLASVIDYVDS-- 705

BLAST of Lsi08G001610 vs. ExPASy TrEMBL
Match: A0A0A0KW06 (Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G647400 PE=3 SV=1)

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 603/691 (87.26%), Postives = 630/691 (91.17%), Query Frame = 0

Query: 2   ETPKPWLICIFFFFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPLP 61
           ET KP  I        FF LLL+A  A VSAQF FPETTSTAV DP KFFSP LLSSPLP
Sbjct: 3   ETSKPSPIST-----LFFLLLLTA--AAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLP 62

Query: 62  TNSFFQNFVLNNGDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALNR 121
           TNSFFQNFVLNNGDLPEYIHPYL+RTAN S+SVSYPSRISNSSIT L FF DL IS+  +
Sbjct: 63  TNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKK 122

Query: 122 TSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDS 181
           T    NTTHFISSFSDLGVDLDIG FRFHLVRGSPYLTFSVLKTSSV I+T NGVRSVDS
Sbjct: 123 T---FNTTHFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDS 182

Query: 182 YEDSTKHIIRLSNGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKI 241
           YED TKHIIRL+NGR+WVLYSSSAIYL+KSK+NQIVTSG F GVIRVAVLP+SA+ESEKI
Sbjct: 183 YEDYTKHIIRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKI 242

Query: 242 LDRYSGCYPVSGFVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYGS 301
           LDRYSGCYPVSGFVKL   FGF+YKWQKKGSGGLLMLAH LHR+IL  +QTVLQN+RY S
Sbjct: 243 LDRYSGCYPVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSS 302

Query: 302 IDGDLLGVVGDSWDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAAS 361
           IDGDLLGVVGDSWDL F+PIPITWHSI+GID KFFPEI+AALKRDVATLN  ELSS  AS
Sbjct: 303 IDGDLLGVVGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPAS 362

Query: 362 YFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGL 421
           YFYGKLLARAARLALIAEEV+  AGVIPAVVKFLK+GIQPWLIGKFPKNGFLYERKW+GL
Sbjct: 363 YFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGL 422

Query: 422 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNF 481
           VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQ+KPQAYAL+ DYMNF
Sbjct: 423 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNF 482

Query: 482 RPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESL 541
           RPKKSQFSIPFRNFDFWKLHSWAAGLTEFP GRNQEST+EAVNAYYAAALMGLAYDDESL
Sbjct: 483 RPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESL 542

Query: 542 TAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWR 601
           TAAGSTLTAAEI ATQTWWHVKRE NGIYD GF EENR+VGILWS  RESRLWFAPAEWR
Sbjct: 543 TAAGSTLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWR 602

Query: 602 ECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKS 661
           ECRVGIQVLPVLPVTERVF+D GFVKEVVEWV PALEREDAGEGWKGFAYALEGIYDKKS
Sbjct: 603 ECRVGIQVLPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKS 662

Query: 662 AVEKVKKLKKHDDGNSLSNLLWWIYSRPEGR 693
           AVEKVKKLKKHDDGNSLSNLLWWIYSRPEGR
Sbjct: 663 AVEKVKKLKKHDDGNSLSNLLWWIYSRPEGR 683

BLAST of Lsi08G001610 vs. ExPASy TrEMBL
Match: A0A5A7TDJ6 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001320 PE=3 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 592/680 (87.06%), Postives = 625/680 (91.91%), Query Frame = 0

Query: 14  FFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPLPTNSFFQNFVLNN 73
           F   FFFLLL+A P  VSAQF FPETTSTAV DP KFFSP LLSSPLPTNSFFQNFVLNN
Sbjct: 10  FSILFFFLLLTAAP--VSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNN 69

Query: 74  GDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFIS 133
           GD PEYIHPYL+RTAN S+ VSYPSRISNSSI  L FF DL IS+LN+T   SN THF+S
Sbjct: 70  GDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATHFVS 129

Query: 134 SFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLS 193
           SFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V I+T NGVRSVDSYED TK+IIRL+
Sbjct: 130 SFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLN 189

Query: 194 NGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPVSG 253
           NGR+W+LYSSSAIYLIKSK+NQ+VTSG F GVIRVAVLP+SA+ESEKILDRYSGCYPVSG
Sbjct: 190 NGRSWLLYSSSAIYLIKSKSNQVVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSG 249

Query: 254 FVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYGSIDGDLLGVVGDS 313
           F KLS  FGF+YKWQKKGSGGLLMLAH LHR+IL  +QTVLQNLRY SIDGDLLGVVGDS
Sbjct: 250 FAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDS 309

Query: 314 WDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGKLLARAAR 373
           WDL F+PIPITWHSI+GID KFFPEI+AALKRDVATLNV ELSST ASYFYGKLLARAAR
Sbjct: 310 WDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARAAR 369

Query: 374 LALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKNGATSTTED 433
           LALIAEEV+Y A VIPAVVKFLK+GIQPWLIGKFPKNGFLYERKW+GLVTKNGATST ED
Sbjct: 370 LALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTIED 429

Query: 434 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPKKSQFSIPFR 493
           FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQ+KPQAYAL+ DYMNFRPKKSQFSIPFR
Sbjct: 430 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFR 489

Query: 494 NFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEI 553
           NFDFWKLHSWAAGL EFP GRNQESTSEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI
Sbjct: 490 NFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEI 549

Query: 554 AATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWRECRVGIQVLPVL 613
            A+QTWWHVK+EKNGIYD GFAEENR+VGILWS  RESRLWFAPAEWRECR+GIQVLPVL
Sbjct: 550 TASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLPVL 609

Query: 614 PVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 673
           PVTER+F+D GFVKE VEWV PALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD
Sbjct: 610 PVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 669

Query: 674 DGNSLSNLLWWIYSRPEGRG 694
           DGNSLSNLLWWIYSRPEGRG
Sbjct: 670 DGNSLSNLLWWIYSRPEGRG 684

BLAST of Lsi08G001610 vs. ExPASy TrEMBL
Match: A0A142BTQ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=glu2 PE=2 SV=1)

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 592/680 (87.06%), Postives = 624/680 (91.76%), Query Frame = 0

Query: 14  FFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPLPTNSFFQNFVLNN 73
           F   FFFLLL+A P  VSAQF FPETTSTAV DP KFFSP LLSSPLPTNSFFQNFVLNN
Sbjct: 10  FSILFFFLLLTAAP--VSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNN 69

Query: 74  GDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFIS 133
           GD PEYIHPYL+RTAN S+ VSYPSRISNSSI  L FF DL IS+LN+T   SN THF+S
Sbjct: 70  GDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATHFVS 129

Query: 134 SFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLS 193
           SFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V I+T NGVRSVDSYED TK+IIRL+
Sbjct: 130 SFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLN 189

Query: 194 NGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPVSG 253
           NGR+WVLYSSSAIYLIKSK+NQIVTSG F GVIRVAVLP+SA+ESEKILDRYSGCYPVSG
Sbjct: 190 NGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSG 249

Query: 254 FVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYGSIDGDLLGVVGDS 313
           F KLS  FGF+YKWQKKGSGGLLMLAH LHR+IL  +QTVLQNLRY SIDGDLLGVVGDS
Sbjct: 250 FAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDS 309

Query: 314 WDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGKLLARAAR 373
           WDL F+PIPITWHSI+GI+ KFFPEI+AALKRDVATLNV ELSST ASYFYGKLLARAAR
Sbjct: 310 WDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARAAR 369

Query: 374 LALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKNGATSTTED 433
           LALIAEEV+Y A VIPAVVKFLK+GIQPWLIGKFPKNGFLYERKW+GLVTKNGATST ED
Sbjct: 370 LALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTIED 429

Query: 434 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPKKSQFSIPFR 493
           FGFGIYNDHHFHLGYFVYSIAVLAKLDPNW KQ+KPQAYAL+ DYMNFRPKKSQFSIPFR
Sbjct: 430 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFSIPFR 489

Query: 494 NFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEI 553
           NFDFWKLHSWAAGL EFP GRNQESTSEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI
Sbjct: 490 NFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEI 549

Query: 554 AATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWRECRVGIQVLPVL 613
            A+QTWWHVK+EKNGIYD GFAEENR+VGILWS  RESRLWFAPAEWRECR+GIQVLPVL
Sbjct: 550 TASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLPVL 609

Query: 614 PVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 673
           PVTER+F+D GFVKE VEWV PALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD
Sbjct: 610 PVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 669

Query: 674 DGNSLSNLLWWIYSRPEGRG 694
           DGNSLSNLLWWIYSRPEGRG
Sbjct: 670 DGNSLSNLLWWIYSRPEGRG 684

BLAST of Lsi08G001610 vs. ExPASy TrEMBL
Match: A0A1S3C9V5 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498635 PE=3 SV=1)

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 592/680 (87.06%), Postives = 624/680 (91.76%), Query Frame = 0

Query: 14  FFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPLPTNSFFQNFVLNN 73
           F   FFFLLL+A P  VSAQF FPETTSTAV DP KFFSP LLSSPLPTNSFFQNFVLNN
Sbjct: 27  FSILFFFLLLTAAP--VSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNN 86

Query: 74  GDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFIS 133
           GD PEYIHPYL+RTAN S+ VSYPSRISNSSI  L FF DL IS+LN+T   SN THF+S
Sbjct: 87  GDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATHFVS 146

Query: 134 SFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLS 193
           SFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V I+T NGVRSVDSYED TK+IIRL+
Sbjct: 147 SFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLN 206

Query: 194 NGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPVSG 253
           NGR+WVLYSSSAIYLIKSK+NQIVTSG F GVIRVAVLP+SA+ESEKILDRYSGCYPVSG
Sbjct: 207 NGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSG 266

Query: 254 FVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYGSIDGDLLGVVGDS 313
           F KLS  FGF+YKWQKKGSGGLLMLAH LHR+IL  +QTVLQNLRY SIDGDLLGVVGDS
Sbjct: 267 FAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDS 326

Query: 314 WDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGKLLARAAR 373
           WDL F+PIPITWHSI+GI+ KFFPEI+AALKRDVATLNV ELSST ASYFYGKLLARAAR
Sbjct: 327 WDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARAAR 386

Query: 374 LALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKNGATSTTED 433
           LALIAEEV+Y A VIPAVVKFLK+GIQPWLIGKFPKNGFLYERKW+GLVTKNGATST ED
Sbjct: 387 LALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTIED 446

Query: 434 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPKKSQFSIPFR 493
           FGFGIYNDHHFHLGYFVYSIAVLAKLDPNW KQ+KPQAYAL+ DYMNFRPKKSQFSIPFR
Sbjct: 447 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFSIPFR 506

Query: 494 NFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEI 553
           NFDFWKLHSWAAGL EFP GRNQESTSEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI
Sbjct: 507 NFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEI 566

Query: 554 AATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWRECRVGIQVLPVL 613
            A+QTWWHVK+EKNGIYD GFAEENR+VGILWS  RESRLWFAPAEWRECR+GIQVLPVL
Sbjct: 567 TASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLPVL 626

Query: 614 PVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 673
           PVTER+F+D GFVKE VEWV PALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD
Sbjct: 627 PVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 686

Query: 674 DGNSLSNLLWWIYSRPEGRG 694
           DGNSLSNLLWWIYSRPEGRG
Sbjct: 687 DGNSLSNLLWWIYSRPEGRG 701

BLAST of Lsi08G001610 vs. ExPASy TrEMBL
Match: A0A6J1EFF1 (Endo-1,3(4)-beta-glucanase OS=Cucurbita moschata OX=3662 GN=LOC111432794 PE=3 SV=1)

HSP 1 Score: 1149.8 bits (2973), Expect = 0.0e+00
Identity = 578/699 (82.69%), Postives = 622/699 (88.98%), Query Frame = 0

Query: 1   METPKPWLICIFFFFFFFFFLLLSAFPAVV---SAQFTFPETTSTAVLDPRKFFSPTLLS 60
           ME P PWL+      FF   LL +AF   +   SAQF FPETTSTAV DP KFFSP+LLS
Sbjct: 1   MEAPNPWLLP-----FFLLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLS 60

Query: 61  SPLPTNSFFQNFVLNNGDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTIS 120
           SPLPTNSFFQNFVLNNGDLPEYIHPYL+RTAN S+SVSYPSR SNSSIT LPF  DLTIS
Sbjct: 61  SPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTIS 120

Query: 121 ALNRTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITT-GNGV 180
           +      VSN THF+SSFSDL VDLDIGDFRFHLVRGSPYLTFSVLKTSS+SI+T GNGV
Sbjct: 121 S------VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKTSSISISTGGNGV 180

Query: 181 RSVDSYEDSTKHIIRLSNGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAM 240
            SVDSY+ STKHIIRLSNGR+WV+YS++AIYL+K K+++IVTSG FSGVIRVAVLPNSA 
Sbjct: 181 GSVDSYDGSTKHIIRLSNGRSWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAA 240

Query: 241 ESEKILDRYSGCYPVSGFVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQN 300
           E+E+ILDRYSGCYPVSG+ KLS NFGF YKWQKKGSGGLLMLAHPLHR++LS N TVLQN
Sbjct: 241 ETEQILDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQN 300

Query: 301 LRYGSIDGDLLGVVGDSWDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELS 360
            +YGSIDGDL+GVVGDSWDLNF PIPITWHSI+GI+RKFFPEI+AALK DV TLNV ELS
Sbjct: 301 FKYGSIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELS 360

Query: 361 STAASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYER 420
           STAASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLK+G+QPWL GKF +NGFLY+R
Sbjct: 361 STAASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDR 420

Query: 421 KWSGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVN 480
           KW+GLVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVLAKLD NWG Q+K  AYALV 
Sbjct: 421 KWAGLVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVY 480

Query: 481 DYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAY 540
           DYMN+R K SQFSIPFRNFDFWKLHSWAAGLTEFP GRNQESTSEA+NAYYAAALMGLAY
Sbjct: 481 DYMNYRSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAY 540

Query: 541 DDESLTAAGSTLTAAEIAATQTWWHVKR-EKNGIYDNGFAEENRVVGILWSGGRESRLWF 600
            DESLTA GS LTAAEIAA QTWWHV R  ++ IYD GF EENRVVGILWSG RESRLWF
Sbjct: 541 GDESLTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWF 600

Query: 601 APAEWRECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEG 660
           A AEWRECRVGIQVLPV+PVTERVF+DEGFVKEVVEWVSPALEREDAGEGWKGF YALEG
Sbjct: 601 ATAEWRECRVGIQVLPVVPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEG 660

Query: 661 IYD-KKSAVEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 694
           IYD K++AV KVKKLKKHDDGNSLSNLLWWIYSRPE +G
Sbjct: 661 IYDNKENAVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 688

BLAST of Lsi08G001610 vs. NCBI nr
Match: XP_038889504.1 (putative endo-1,3(4)-beta-glucanase 2 [Benincasa hispida])

HSP 1 Score: 1280.8 bits (3313), Expect = 0.0e+00
Identity = 633/693 (91.34%), Postives = 658/693 (94.95%), Query Frame = 0

Query: 1   METPKPWLICIFFFFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPL 60
           METPKPWLI I    FFFFF++L+AFP  VS QF FP+TTSTAV DP KFFSPTLLSSPL
Sbjct: 23  METPKPWLISI----FFFFFIILTAFPPAVSGQFLFPQTTSTAVPDPAKFFSPTLLSSPL 82

Query: 61  PTNSFFQNFVLNNGDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALN 120
           PTNSFFQNFVLNNGD PEYIHPY+VRTAN S+SVSYPSRISNSSIT L F+ DLTISAL+
Sbjct: 83  PTNSFFQNFVLNNGDKPEYIHPYIVRTANSSLSVSYPSRISNSSITQLQFYPDLTISALD 142

Query: 121 RTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVD 180
           RTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLK SSVSI+TGNGVRSVD
Sbjct: 143 RTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKISSVSISTGNGVRSVD 202

Query: 181 SYEDSTKHIIRLSNGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEK 240
           SY+DSTKHIIRL+NGRNWVLYSSSAIYLIKSKNNQIVTSG FSGVIR+AVLPNSAMES  
Sbjct: 203 SYDDSTKHIIRLNNGRNWVLYSSSAIYLIKSKNNQIVTSGGFSGVIRIAVLPNSAMESGN 262

Query: 241 ILDRYSGCYPVSGFVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYG 300
           ILDRYSGCYPVSG+VKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILS NQTVL NLRYG
Sbjct: 263 ILDRYSGCYPVSGYVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSRNQTVLPNLRYG 322

Query: 301 SIDGDLLGVVGDSWDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAA 360
           SIDGDLLGVVGDSWDLNF+P+PITWHSI+GID+KFFPEI+AALKRDVATLNV ELSSTA+
Sbjct: 323 SIDGDLLGVVGDSWDLNFNPVPITWHSINGIDKKFFPEIVAALKRDVATLNVTELSSTAS 382

Query: 361 SYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSG 420
           SYFY KLLARAARLALIAEEVD  AGVIPAVV+FLK+GIQPWLIGKFPKNGFLYERKW+G
Sbjct: 383 SYFYAKLLARAARLALIAEEVDNGAGVIPAVVRFLKNGIQPWLIGKFPKNGFLYERKWAG 442

Query: 421 LVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMN 480
           LVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALV DYMN
Sbjct: 443 LVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVYDYMN 502

Query: 481 FRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDES 540
           FRPKKSQFSIPFRNFDFWKLHSWAAGLTEFP GRNQESTSEAVNAYYAAALMGLAYDDES
Sbjct: 503 FRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAVNAYYAAALMGLAYDDES 562

Query: 541 LTAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEW 600
           LTAAGSTLTAAEIAATQTWWHVK EKN IYD GF EENRVVGILWS  RESRLWFAPAEW
Sbjct: 563 LTAAGSTLTAAEIAATQTWWHVKSEKNWIYDKGFEEENRVVGILWSAARESRLWFAPAEW 622

Query: 601 RECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKK 660
           RECRVGIQVLPVLPV+ERVFSD+GFVKEVVEWVS ALEREDAGEGWKGFAYALEGIYDKK
Sbjct: 623 RECRVGIQVLPVLPVSERVFSDKGFVKEVVEWVSRALEREDAGEGWKGFAYALEGIYDKK 682

Query: 661 SAVEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 694
           SA+EKVKKLKKHDDGNSLSNLLWWIYSRPEGRG
Sbjct: 683 SAIEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 711

BLAST of Lsi08G001610 vs. NCBI nr
Match: XP_011656051.1 (putative endo-1,3(4)-beta-glucanase 2 [Cucumis sativus] >KGN52617.1 hypothetical protein Csa_008831 [Cucumis sativus])

HSP 1 Score: 1198.7 bits (3100), Expect = 0.0e+00
Identity = 603/691 (87.26%), Postives = 630/691 (91.17%), Query Frame = 0

Query: 2   ETPKPWLICIFFFFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPLP 61
           ET KP  I        FF LLL+A  A VSAQF FPETTSTAV DP KFFSP LLSSPLP
Sbjct: 3   ETSKPSPIST-----LFFLLLLTA--AAVSAQFPFPETTSTAVPDPGKFFSPNLLSSPLP 62

Query: 62  TNSFFQNFVLNNGDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALNR 121
           TNSFFQNFVLNNGDLPEYIHPYL+RTAN S+SVSYPSRISNSSIT L FF DL IS+  +
Sbjct: 63  TNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRISNSSITQLQFFPDLVISSPKK 122

Query: 122 TSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDS 181
           T    NTTHFISSFSDLGVDLDIG FRFHLVRGSPYLTFSVLKTSSV I+T NGVRSVDS
Sbjct: 123 T---FNTTHFISSFSDLGVDLDIGVFRFHLVRGSPYLTFSVLKTSSVLISTSNGVRSVDS 182

Query: 182 YEDSTKHIIRLSNGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKI 241
           YED TKHIIRL+NGR+WVLYSSSAIYL+KSK+NQIVTSG F GVIRVAVLP+SA+ESEKI
Sbjct: 183 YEDYTKHIIRLNNGRSWVLYSSSAIYLVKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKI 242

Query: 242 LDRYSGCYPVSGFVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYGS 301
           LDRYSGCYPVSGFVKL   FGF+YKWQKKGSGGLLMLAH LHR+IL  +QTVLQN+RY S
Sbjct: 243 LDRYSGCYPVSGFVKLLGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNIRYSS 302

Query: 302 IDGDLLGVVGDSWDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAAS 361
           IDGDLLGVVGDSWDL F+PIPITWHSI+GID KFFPEI+AALKRDVATLN  ELSS  AS
Sbjct: 303 IDGDLLGVVGDSWDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNATELSSMPAS 362

Query: 362 YFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGL 421
           YFYGKLLARAARLALIAEEV+  AGVIPAVVKFLK+GIQPWLIGKFPKNGFLYERKW+GL
Sbjct: 363 YFYGKLLARAARLALIAEEVNGGAGVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGL 422

Query: 422 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNF 481
           VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQ+KPQAYAL+ DYMNF
Sbjct: 423 VTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNF 482

Query: 482 RPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESL 541
           RPKKSQFSIPFRNFDFWKLHSWAAGLTEFP GRNQEST+EAVNAYYAAALMGLAYDDESL
Sbjct: 483 RPKKSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTTEAVNAYYAAALMGLAYDDESL 542

Query: 542 TAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWR 601
           TAAGSTLTAAEI ATQTWWHVKRE NGIYD GF EENR+VGILWS  RESRLWFAPAEWR
Sbjct: 543 TAAGSTLTAAEITATQTWWHVKRENNGIYDKGFTEENRMVGILWSAARESRLWFAPAEWR 602

Query: 602 ECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKS 661
           ECRVGIQVLPVLPVTERVF+D GFVKEVVEWV PALEREDAGEGWKGFAYALEGIYDKKS
Sbjct: 603 ECRVGIQVLPVLPVTERVFTDVGFVKEVVEWVQPALEREDAGEGWKGFAYALEGIYDKKS 662

Query: 662 AVEKVKKLKKHDDGNSLSNLLWWIYSRPEGR 693
           AVEKVKKLKKHDDGNSLSNLLWWIYSRPEGR
Sbjct: 663 AVEKVKKLKKHDDGNSLSNLLWWIYSRPEGR 683

BLAST of Lsi08G001610 vs. NCBI nr
Match: KAA0039339.1 (putative endo-1,3(4)-beta-glucanase 2 precursor [Cucumis melo var. makuwa] >TYK00522.1 putative endo-1,3(4)-beta-glucanase 2 precursor [Cucumis melo var. makuwa])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 592/680 (87.06%), Postives = 625/680 (91.91%), Query Frame = 0

Query: 14  FFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPLPTNSFFQNFVLNN 73
           F   FFFLLL+A P  VSAQF FPETTSTAV DP KFFSP LLSSPLPTNSFFQNFVLNN
Sbjct: 10  FSILFFFLLLTAAP--VSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNN 69

Query: 74  GDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFIS 133
           GD PEYIHPYL+RTAN S+ VSYPSRISNSSI  L FF DL IS+LN+T   SN THF+S
Sbjct: 70  GDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATHFVS 129

Query: 134 SFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLS 193
           SFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V I+T NGVRSVDSYED TK+IIRL+
Sbjct: 130 SFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLN 189

Query: 194 NGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPVSG 253
           NGR+W+LYSSSAIYLIKSK+NQ+VTSG F GVIRVAVLP+SA+ESEKILDRYSGCYPVSG
Sbjct: 190 NGRSWLLYSSSAIYLIKSKSNQVVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSG 249

Query: 254 FVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYGSIDGDLLGVVGDS 313
           F KLS  FGF+YKWQKKGSGGLLMLAH LHR+IL  +QTVLQNLRY SIDGDLLGVVGDS
Sbjct: 250 FAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDS 309

Query: 314 WDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGKLLARAAR 373
           WDL F+PIPITWHSI+GID KFFPEI+AALKRDVATLNV ELSST ASYFYGKLLARAAR
Sbjct: 310 WDLKFNPIPITWHSINGIDSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARAAR 369

Query: 374 LALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKNGATSTTED 433
           LALIAEEV+Y A VIPAVVKFLK+GIQPWLIGKFPKNGFLYERKW+GLVTKNGATST ED
Sbjct: 370 LALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTIED 429

Query: 434 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPKKSQFSIPFR 493
           FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQ+KPQAYAL+ DYMNFRPKKSQFSIPFR
Sbjct: 430 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQYKPQAYALLYDYMNFRPKKSQFSIPFR 489

Query: 494 NFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEI 553
           NFDFWKLHSWAAGL EFP GRNQESTSEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI
Sbjct: 490 NFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEI 549

Query: 554 AATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWRECRVGIQVLPVL 613
            A+QTWWHVK+EKNGIYD GFAEENR+VGILWS  RESRLWFAPAEWRECR+GIQVLPVL
Sbjct: 550 TASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLPVL 609

Query: 614 PVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 673
           PVTER+F+D GFVKE VEWV PALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD
Sbjct: 610 PVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 669

Query: 674 DGNSLSNLLWWIYSRPEGRG 694
           DGNSLSNLLWWIYSRPEGRG
Sbjct: 670 DGNSLSNLLWWIYSRPEGRG 684

BLAST of Lsi08G001610 vs. NCBI nr
Match: NP_001315392.1 (putative endo-1,3(4)-beta-glucanase 2 precursor [Cucumis melo] >AMP41497.1 beta-1,3-glucanase 2 [Cucumis melo] >ANB86004.1 beta-1,3-glucanase 2 [Cucumis melo] >ANB86008.1 beta-1,3-glucanase 2 [Cucumis melo] >AXM43892.1 beta-1,3-glucanase A [Cucumis melo] >AXM43893.1 beta-1,3-glucanase A [Cucumis melo])

HSP 1 Score: 1184.9 bits (3064), Expect = 0.0e+00
Identity = 592/680 (87.06%), Postives = 624/680 (91.76%), Query Frame = 0

Query: 14  FFFFFFFLLLSAFPAVVSAQFTFPETTSTAVLDPRKFFSPTLLSSPLPTNSFFQNFVLNN 73
           F   FFFLLL+A P  VSAQF FPETTSTAV DP KFFSP LLSSPLPTNSFFQNFVLNN
Sbjct: 10  FSILFFFLLLTAAP--VSAQFPFPETTSTAVPDPSKFFSPNLLSSPLPTNSFFQNFVLNN 69

Query: 74  GDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFIS 133
           GD PEYIHPYL+RTAN S+ VSYPSRISNSSI  L FF DL IS+LN+T   SN THF+S
Sbjct: 70  GDSPEYIHPYLIRTANSSLFVSYPSRISNSSINQLQFFPDLVISSLNKT---SNATHFVS 129

Query: 134 SFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLS 193
           SFSDLGVDLDIG FR+HLVRGSPYLTFSVLKTS V I+T NGVRSVDSYED TK+IIRL+
Sbjct: 130 SFSDLGVDLDIGVFRYHLVRGSPYLTFSVLKTSFVLISTSNGVRSVDSYEDYTKYIIRLN 189

Query: 194 NGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPVSG 253
           NGR+WVLYSSSAIYLIKSK+NQIVTSG F GVIRVAVLP+SA+ESEKILDRYSGCYPVSG
Sbjct: 190 NGRSWVLYSSSAIYLIKSKSNQIVTSGGFIGVIRVAVLPDSAVESEKILDRYSGCYPVSG 249

Query: 254 FVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQNLRYGSIDGDLLGVVGDS 313
           F KLS  FGF+YKWQKKGSGGLLMLAH LHR+IL  +QTVLQNLRY SIDGDLLGVVGDS
Sbjct: 250 FAKLSGGFGFEYKWQKKGSGGLLMLAHTLHREILPRDQTVLQNLRYSSIDGDLLGVVGDS 309

Query: 314 WDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGKLLARAAR 373
           WDL F+PIPITWHSI+GI+ KFFPEI+AALKRDVATLNV ELSST ASYFYGKLLARAAR
Sbjct: 310 WDLKFNPIPITWHSINGINSKFFPEIVAALKRDVATLNVTELSSTPASYFYGKLLARAAR 369

Query: 374 LALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKNGATSTTED 433
           LALIAEEV+Y A VIPAVVKFLK+GIQPWLIGKFPKNGFLYERKW+GLVTKNGATST ED
Sbjct: 370 LALIAEEVNYGASVIPAVVKFLKNGIQPWLIGKFPKNGFLYERKWAGLVTKNGATSTIED 429

Query: 434 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPKKSQFSIPFR 493
           FGFGIYNDHHFHLGYFVYSIAVLAKLDPNW KQ+KPQAYAL+ DYMNFRPKKSQFSIPFR
Sbjct: 430 FGFGIYNDHHFHLGYFVYSIAVLAKLDPNWRKQYKPQAYALLYDYMNFRPKKSQFSIPFR 489

Query: 494 NFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEI 553
           NFDFWKLHSWAAGL EFP GRNQESTSEAVNAYYAAALMGLAY D SLTAAGSTLTAAEI
Sbjct: 490 NFDFWKLHSWAAGLAEFPDGRNQESTSEAVNAYYAAALMGLAYGDASLTAAGSTLTAAEI 549

Query: 554 AATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWRECRVGIQVLPVL 613
            A+QTWWHVK+EKNGIYD GFAEENR+VGILWS  RESRLWFAPAEWRECR+GIQVLPVL
Sbjct: 550 TASQTWWHVKKEKNGIYDKGFAEENRMVGILWSAARESRLWFAPAEWRECRLGIQVLPVL 609

Query: 614 PVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 673
           PVTER+F+D GFVKE VEWV PALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD
Sbjct: 610 PVTERMFTDVGFVKEAVEWVQPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHD 669

Query: 674 DGNSLSNLLWWIYSRPEGRG 694
           DGNSLSNLLWWIYSRPEGRG
Sbjct: 670 DGNSLSNLLWWIYSRPEGRG 684

BLAST of Lsi08G001610 vs. NCBI nr
Match: KAG7025154.1 (hypothetical protein SDJN02_13977, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 580/698 (83.09%), Postives = 621/698 (88.97%), Query Frame = 0

Query: 1   METPKPWLICIFFFFFFFFFLLLSAFPAVV---SAQFTFPETTSTAVLDPRKFFSPTLLS 60
           ME P PWL+       FF  LL +AF   +   SAQF FPETTSTAV DP KFFSP+LLS
Sbjct: 1   MEAPNPWLLP------FFLLLLAAAFSDQLPPSSAQFPFPETTSTAVPDPTKFFSPSLLS 60

Query: 61  SPLPTNSFFQNFVLNNGDLPEYIHPYLVRTANYSISVSYPSRISNSSITLLPFFSDLTIS 120
           SPLPTNSFFQNFVLNNGDLPEYIHPYL+RTAN S+SVSYPSR SNSSIT LPF  DLTIS
Sbjct: 61  SPLPTNSFFQNFVLNNGDLPEYIHPYLIRTANSSLSVSYPSRTSNSSITQLPFSPDLTIS 120

Query: 121 ALNRTSPVSNTTHFISSFSDLGVDLDIGDFRFHLVRGSPYLTFSVLKTSSVSITT-GNGV 180
           +      VSN THF+SSFSDL VDLDIGDFRFHLVRGSPYLTFSVLK SSVSI+T GNGV
Sbjct: 121 S------VSNKTHFVSSFSDLAVDLDIGDFRFHLVRGSPYLTFSVLKNSSVSISTGGNGV 180

Query: 181 RSVDSYEDSTKHIIRLSNGRNWVLYSSSAIYLIKSKNNQIVTSGVFSGVIRVAVLPNSAM 240
            SVDSY+DSTKHIIRLSNGR WV+YS++AIYL+K K+++IVTSG FSGVIRVAVLPNSA 
Sbjct: 181 GSVDSYDDSTKHIIRLSNGRTWVVYSTAAIYLMKGKSSEIVTSGGFSGVIRVAVLPNSAA 240

Query: 241 ESEKILDRYSGCYPVSGFVKLSENFGFKYKWQKKGSGGLLMLAHPLHRQILSGNQTVLQN 300
           E+E+ILDRYSGCYPVSG+ KLS NFGF YKWQKKGSGGLLMLAHPLHR++LS N TVLQN
Sbjct: 241 ETEQILDRYSGCYPVSGYAKLSGNFGFSYKWQKKGSGGLLMLAHPLHRRVLSENLTVLQN 300

Query: 301 LRYGSIDGDLLGVVGDSWDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELS 360
            +YGSIDGDL+GVVGDSWDLNF PIPITWHSI+GI+RKFFPEI+AALK DV TLNV ELS
Sbjct: 301 FKYGSIDGDLVGVVGDSWDLNFAPIPITWHSINGIERKFFPEIVAALKHDVGTLNVTELS 360

Query: 361 STAASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYER 420
           STAASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLK+G+QPWL GKF +NGFLY+R
Sbjct: 361 STAASYFYGKLLARAARLALIAEEVDYAAGVIPAVVKFLKTGVQPWLDGKFGRNGFLYDR 420

Query: 421 KWSGLVTKNGATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVN 480
           KW+GLVTKNGATS TEDFGFGIYNDHHFHLGYFVYSIAVLAKLD NWG Q+K  AYALV 
Sbjct: 421 KWAGLVTKNGATSKTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDRNWGNQYKAPAYALVY 480

Query: 481 DYMNFRPKKSQFSIPFRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAY 540
           DYMN+R K SQFSIPFRNFDFWKLHSWAAGLTEFP GRNQESTSEA+NAYYAAALMGLAY
Sbjct: 481 DYMNYRSKNSQFSIPFRNFDFWKLHSWAAGLTEFPDGRNQESTSEAINAYYAAALMGLAY 540

Query: 541 DDESLTAAGSTLTAAEIAATQTWWHVKR-EKNGIYDNGFAEENRVVGILWSGGRESRLWF 600
            DESLTA GS LTAAEIAA QTWWHV R  ++ IYD GF EENRVVGILWSG RESRLWF
Sbjct: 541 GDESLTAVGSALTAAEIAAAQTWWHVNRGGRSSIYDEGFTEENRVVGILWSGARESRLWF 600

Query: 601 APAEWRECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEG 660
           A AEWRECRVGIQVLPVLPVTERVF+DEGFVKEVVEWVSPALEREDAGEGWKGF YALEG
Sbjct: 601 ATAEWRECRVGIQVLPVLPVTERVFADEGFVKEVVEWVSPALEREDAGEGWKGFVYALEG 660

Query: 661 IYDKKSAVEKVKKLKKHDDGNSLSNLLWWIYSRPEGRG 694
           IYDK++AV KVKKLKKHDDGNSLSNLLWWIYSRPE +G
Sbjct: 661 IYDKENAVGKVKKLKKHDDGNSLSNLLWWIYSRPERQG 686

BLAST of Lsi08G001610 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 724.2 bits (1868), Expect = 1.0e-208
Identity = 372/663 (56.11%), Postives = 467/663 (70.44%), Query Frame = 0

Query: 34  FTFPETTSTAVLDPRKFFSPTLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLVRTANYSIS 93
           F FP + S+ + DP +FFS  LLSSPLPTNSFFQNF LNNGD  EY HPY+++ +  S+S
Sbjct: 55  FLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSSLS 114

Query: 94  VSYPSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFISSFSDLGVDLDI--GDFRFHL 153
           +SYPS   NS+     F +D+TI+  +   P S  +H ISSFSDLGV LD    + RF L
Sbjct: 115 ISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRFFL 174

Query: 154 VRGSPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLSNGRNWVLYSSSAIYLIKS 213
           VRGSP++TFSV   SS++I+T + V S+     STK+ ++L+N + W++Y+SS I L K 
Sbjct: 175 VRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLTKD 234

Query: 214 KNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPVSGFVKLSENFGFKYKWQKKG 273
             + I     FSG+IR+ VLPN     E ILD +S  YPVSG    ++ F  +YKW+K+G
Sbjct: 235 GVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEKRG 294

Query: 274 SGGLLMLAHPLHRQILSGNQ---TVLQNLRYGSIDGDLLGVVGDSWDLNFHPIPITWHSI 333
            G LLMLAHPLH ++LS N    TVL N +Y SIDGDL+GV+GDSW L   P+ +TWHSI
Sbjct: 295 YGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWHSI 354

Query: 334 DGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYGKLLARAARLALIAEEVDYAAGVI 393
            G+      EII+AL +DV  L+ +    T +SYFY KL+ARAARLALIAEEV Y   VI
Sbjct: 355 KGVQEDSHQEIISALIKDVNALD-SSAEVTNSSYFYAKLIARAARLALIAEEVCY-LDVI 414

Query: 394 PAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKNGATSTTEDFGFGIYNDHHFHLGY 453
           P +  +LK+ I+PWL G F  NGFLY+ KW G++TK G+  +  DFGFGIYNDHH+HLGY
Sbjct: 415 PKIRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGY 474

Query: 454 FVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPK--KSQFSIP-FRNFDFWKLHSWAA 513
           FVY+IAVLAK+DP WGK+++PQAY L+ DY+    K  KS  + P  R FD +KLHSWA 
Sbjct: 475 FVYAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAG 534

Query: 514 GLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESLTAAGSTLTAAEIAATQTWWHVKRE 573
           GLTEF  GRNQESTSEAVNAYY+AAL+GLAY D  L AA S +   EI A + WW VK E
Sbjct: 535 GLTEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVK-E 594

Query: 574 KNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWRECRVGIQVLPVLPVTERVFSDEGF 633
            + IY   F  ENRVVG+LWS  R+S LWFAP EW+ECR+GIQ+LP+LPV+E +FSD  F
Sbjct: 595 DDAIYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTF 654

Query: 634 VKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKSAVEKVKKLKKHDDGNSLSNLLWWI 689
           VK++V W  PAL R+  GEGWKGF YALE +YDK  A+EK+K L   DDGNSLSNLLWW+
Sbjct: 655 VKQLVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWWV 712

BLAST of Lsi08G001610 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 671.0 bits (1730), Expect = 1.0e-192
Identity = 351/687 (51.09%), Postives = 444/687 (64.63%), Query Frame = 0

Query: 31  SAQFTFPETTSTAVLDPRKFFSPTLLSSPLPTNSFFQNFVLNNGDLPEYIHPYLVRTANY 90
           +A F FPET S+ + DP +FFSP LLS+PLPTNSFFQNF L NGD  EY HPYL+++ + 
Sbjct: 5   NALFLFPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDS 64

Query: 91  SISVSYPSRISNSSITLLPFFSDLTISALNRTSPVSNTTHFISSFSDLGVDLDI--GDFR 150
           S+ +SYPS   NS      F +D+ IS  N   P S  TH ISSFSDL V LD    + R
Sbjct: 65  SLCISYPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLR 124

Query: 151 FHLVRGSPYLTFSVLKTSSVSITTGNGVRSVDSYEDSTKHIIRLSNGRNWVLYSSSAIYL 210
           F LVRGSP                            STK   +L+N + W++Y+SS I L
Sbjct: 125 FFLVRGSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDL 184

Query: 211 IKSKNNQIVTSGVFSGVIRVAVLPNSAMESEKILDRYSGCYPVSGFVKLSENFGFKYKWQ 270
            K  ++ I   G F+G++R+ VLP S    E  LDR+S CYPVSG    ++ F  KY W+
Sbjct: 185 TKHGDSSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWE 244

Query: 271 KKGSGGLLMLAHPLHRQIL-----------------------SGNQTVLQNLRYGSIDGD 330
           K+GSG LLMLAHPLH ++L                       + + TVL + RY SIDGD
Sbjct: 245 KRGSGDLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGD 304

Query: 331 LLGVVGDSWDLNFHPIPITWHSIDGIDRKFFPEIIAALKRDVATLNVAELSSTAASYFYG 390
           L+GVVGDSW L    + +TWHS+ G+ +  + EII+AL +DV  LN +    T++SYFYG
Sbjct: 305 LVGVVGDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLN-SSAEVTSSSYFYG 364

Query: 391 KLLARAARLALIAEEVDYAAGVIPAVVKFLKSGIQPWLIGKFPKNGFLYERKWSGLVTKN 450
           KL+ARAAR ALIAEEV Y   VIP +V +LK+ I+PWL G F  NGFLY+ KW GL+TK 
Sbjct: 365 KLIARAARFALIAEEVCY-LDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQ 424

Query: 451 GATSTTEDFGFGIYNDHHFHLGYFVYSIAVLAKLDPNWGKQFKPQAYALVNDYMNFRPK- 510
           G+  +  DFGFGIYNDHH+H+GYF+Y+IAVLAK DP WG++++ QAY+L+ D+M F  K 
Sbjct: 425 GSKDSQADFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKD 484

Query: 511 --KSQFSIP-FRNFDFWKLHSWAAGLTEFPFGRNQESTSEAVNAYYAAALMGLAYDDESL 570
              S  S P  RNFD +KLHSWA GLTEF  GRNQESTSEAVNAYY+AAL+GLAY D+ L
Sbjct: 485 DNNSNSSYPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHL 544

Query: 571 TAAGSTLTAAEIAATQTWWHVKREKNGIYDNGFAEENRVVGILWSGGRESRLWFAPAEWR 630
               ST+   EI A + WW VK+ +  +Y   F  ENRVVG+LWS  R+S LWF P EW+
Sbjct: 545 VETASTIMTLEIHAAKMWWQVKKGE-ALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWK 604

Query: 631 ECRVGIQVLPVLPVTERVFSDEGFVKEVVEWVSPALEREDAGEGWKGFAYALEGIYDKKS 689
           ECR+GIQ+LP+LP              +V W  PAL+R   GEGWKGF YALE +YDK  
Sbjct: 605 ECRLGIQLLPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDG 648