Homology
BLAST of Lsi08G001180 vs. ExPASy Swiss-Prot
Match:
Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)
HSP 1 Score: 548.9 bits (1413), Expect = 2.0e-154
Identity = 361/932 (38.73%), Postives = 532/932 (57.08%), Query Frame = 0
Query: 35 NYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAEKMKAVEESRSSI 94
+YDVFLS R +DT ++FT+ L+E L +GI F+DD + G E KA+EES+ +I
Sbjct: 11 SYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAI 70
Query: 95 VVFSENYGNLA-CMKEVGKIVMCKELRDQLVLPIFYQIDPANVRKQKGNFEKHFNEHEAN 154
VVFSENY C+ E+ KI+ CK Q V+PIFY +DP++VR QK +F K F EHE
Sbjct: 71 VVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHETK 130
Query: 155 LGIDNEEVQNWRYSMNQLGHLSG-----------------------------WHLQ---- 214
D E +Q WR ++N+ +L G +LQ
Sbjct: 131 YKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKISLSYLQNIVG 190
Query: 215 -DSQLHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKSV------SHLFEGCYFLD 274
D+ L ++ LL IG++ +R +GIWGMGG+GKTT+AR I+ ++ S+ F+G FL
Sbjct: 191 IDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLK 250
Query: 275 NVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGATLIKRRISSIKALIVLDDVNHLSQ 334
++KE G+ SLQ LL+ L ++ DG + R+ S K LIVLDD+++
Sbjct: 251 DIKE--NKRGMHSLQNALLSELLREKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDH 310
Query: 335 -LQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTNH 394
L+ LAG DWFG GSR+IITTRD+HL+ + I Y+V L E++QLF Q AFG
Sbjct: 311 YLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDI--IYEVTALPDHESIQLFKQHAFGKEV 370
Query: 395 PKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKIS 454
P + + LS++VV Y LPLA++V GS L + + W++A+E +K I + LKIS
Sbjct: 371 PNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKIS 430
Query: 455 YDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLI-TTPHEKIQ 514
YD LE +QE+FLD+ACF + + K +++L+S A GL IL +KSL+ + + ++Q
Sbjct: 431 YDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQ 490
Query: 515 MHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVMDLSEEGESQ 574
MHDLIQ+MG+ IV F +P +RSRLWL K+V +S++ GT A++ I + S
Sbjct: 491 MHDLIQDMGKYIVN--FQKDPGERSRLWLAKEVEEVMSNNTGTMAMEAIWV------SSY 550
Query: 575 LNAKAFS--AMTNLKMLKINNVYLSGD---LEYLSDQLRFLNWHGYPLEVHVN-YQLS-Y 634
+ FS A+ N+K L++ N+ S ++YL + LR YP E + ++L
Sbjct: 551 SSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPWESFPSTFELKML 610
Query: 635 VFLVISYYCLKY------RFDKLKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKL 694
V L + + L++ L+ I+LS S+ +++TPDF G+PNLE + L C+ L ++
Sbjct: 611 VHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEV 670
Query: 695 HQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKFFPKIVGNMKNLIEF 754
H SLG +I L L +CK LK P +++ESL L L C L+ P+I G MK I+
Sbjct: 671 HHSLGCCSKVIGLYLNDCKSLKRFPC-VNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQI 730
Query: 755 HLDGTSIQELHPSIGQL-TGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGCSKLDRIP 814
H+ G+ I+EL SI Q T + L L N NL+ LP++I L SL +L++ GCSKL+ +P
Sbjct: 731 HMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKSLVSLSVSGCSKLESLP 790
Query: 815 ESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPSWNYSSHYSS 874
E +G + L D + T I + P S+ L L IL +G + +H FP +
Sbjct: 791 EEIGDLDNLRVFDASDTLILRPPSSIIRLNKLIILMFRGF-KDGVHFEFPP-------VA 850
Query: 875 QGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSGNNFTCLPKSLGQL 910
+GL S+ LNLS C L+DG +P + SL S++ LDLS NNF LP S+ QL
Sbjct: 851 EGLH---------SLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSRNNFEHLPSSIAQL 910
BLAST of Lsi08G001180 vs. ExPASy Swiss-Prot
Match:
V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)
HSP 1 Score: 531.9 bits (1369), Expect = 2.5e-149
Identity = 399/1152 (34.64%), Postives = 590/1152 (51.22%), Query Frame = 0
Query: 7 SMERGASITSLSSPPSPPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVV 66
S R +S +S SS PS +P YDVFLS R +DT +FT L+ AL +GI
Sbjct: 5 SSSRASSSSSSSSTPS-------IPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRT 64
Query: 67 FRDDVDEDDGGKPLA-EKMKAVEESRSSIVVFSENYGNLA-CMKEVGKIVMC-KELRD-- 126
FRD D+ G+ +A E +KA+EESRSS++VFSENY C+ E+ KI+ C K+ +D
Sbjct: 65 FRD--DKLRRGEAIAPELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPG 124
Query: 127 QLVLPIFYQIDPANVRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQD 186
V PIFY +DP++VRKQ+G+F + F + NL +++ WR ++ + +LSGW LQD
Sbjct: 125 HAVFPIFYHVDPSHVRKQEGSFGEAFAGYGENL---KDKIPRWRTALTEAANLSGWPLQD 184
Query: 187 S-QLHQVNRLLG-----------------IGLD---------------DIRFVGIWGMGG 246
+ +Q+ + +G+D D+R VG++G+GG
Sbjct: 185 GYESNQIKEITDSIFRRLKCKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGG 244
Query: 247 IGKTTLARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPN--A 306
IGKTT+A++IY +S FE FL+N++E +G++ LQ +LL L + N A
Sbjct: 245 IGKTTIAKVIYNELSREFEYMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNINSVA 304
Query: 307 DGATLIKRRISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIE 366
GA++IK +SS IVLDDV+ SQL+ L +W G GSRVIITTR++H+L ++
Sbjct: 305 HGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQKVD 364
Query: 367 RRYKVEGLNVEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKS 426
Y+V+GLN EEA +LFS AF N PK Y +LS +VV Y LPLA++VLG L K+
Sbjct: 365 DLYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKT 424
Query: 427 MDVWENAVEKLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSF 486
+ WE+ + KL + +I +LK SYD L E+ IFLD+ACFFK + + ++L +
Sbjct: 425 IPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDAC 484
Query: 487 GFAAIIGLEILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNL 546
F A IG++ L +K LIT + +I+MHDLIQ+MG EIVR FP+ P K SRLW D
Sbjct: 485 DFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFER 544
Query: 547 ALSHDQGTEAIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINN--------VYLSGD-- 606
AL+ +G + ++ I +DLS+ N+ AF+ MT L++LK+ + Y+ D
Sbjct: 545 ALTAYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDK 604
Query: 607 -------------------LEYLSDQLRFLNWHGYPLEVHVNYQLSYVFLVISYYCLKYR 666
++ S +LR+L W GYPL+ + + + C +
Sbjct: 605 VELYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIK 664
Query: 667 --------FDKLKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLHQSLGTLKHLI 726
+ LK I+LS S+ +S+ +F +PNLERL L GC L +H S+G +K L
Sbjct: 665 QLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLT 724
Query: 727 LLDLKNCKCLKDIPFNI-SLESLVILTLSGCSRLKFFPKIVGNMKNLIEFHLDGTSIQEL 786
L LK+CK LK++P +I LESL IL L+ CS+ + FP+ GNMK+L E L T+I++L
Sbjct: 725 TLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKDL 784
Query: 787 HPSIGQLTGLVLLNLENC-----------------------TNLLELPNTIGSLISLKTL 846
SIG L L L+L +C T + +LP++I L SL+ L
Sbjct: 785 PDSIGDLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNTAIKDLPDSIRDLESLERL 844
Query: 847 TLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSL 906
L CSK ++ PE G + L +LD+ T I P S+ L +L+ LD S KF
Sbjct: 845 YLSYCSKFEKFPEKGGNMKSLMELDLQNTAIKDLPDSIGDLESLKYLDLSNCS-KFEKFP 904
Query: 907 FPSWNYSS----HYSSQGLK-LTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQIL 966
N S + +K L S+ LNLSDC + P ++ S+ L
Sbjct: 905 EKGGNMKSLTELFLENTAIKDLPDSIGDLESLVSLNLSDCSKFE-KFPEKGGNMKSLNWL 964
Query: 967 DLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLP--------LSVRNVEARDCV-S 1026
L+ LP S+G L SL L L N + ++LP+ L +RN +D S
Sbjct: 965 YLNNTAIKDLPDSIGDLESLMRLYLSNSSKFEKLPEKVGNMKSLELLDLRNTAIKDLPDS 1024
Query: 1027 LKEYYNQEKQMPSSSTGMAVISCPITNEEKNFKIDRVNLSSI----HLRTMVQRYIEVLT 1039
+ + EK S+ V+ + + + + +LS + H + E
Sbjct: 1025 IGDLEPLEKLSLSNCPKFEVLPLSLKAIDAHLCTSKEDLSRLLWLCHRNWLKSTTEEFDR 1084
BLAST of Lsi08G001180 vs. ExPASy Swiss-Prot
Match:
V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)
HSP 1 Score: 528.5 bits (1360), Expect = 2.7e-148
Identity = 364/997 (36.51%), Postives = 539/997 (54.06%), Query Frame = 0
Query: 18 SSPPSPPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGG 77
SS S PS +P YDVFLS R +DT +FT L+ AL +GI FRD D G
Sbjct: 10 SSSSSTPS----IPRTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRD--DRLRRG 69
Query: 78 KPLA-EKMKAVEESRSSIVVFSENYGNLA-CMKEVGKIVMCKELRDQLVLPIFYQIDPAN 137
+ +A E +KA+EESRSS++VFSENY + C+ E+ KI+ C++ V PIFY +DP++
Sbjct: 70 EAIAPELLKAIEESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSH 129
Query: 138 VRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQDS--QLHQVNRLLG- 197
VRKQ+G+F + F +E N +++ WR ++ + +LSGWHL D + +Q+ +
Sbjct: 130 VRKQEGSFGEAFAGYEENW---KDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNS 189
Query: 198 ----------------IGLD---------------DIRFVGIWGMGGIGKTTLARIIYKS 257
+G+D D+R VGI+G+GGIGKTT+A++IY
Sbjct: 190 IFRQLKCKRLDVGANLVGIDSHVKEMILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYNE 249
Query: 258 VSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALM---KRNIEIPNADGATLIKRRISS 317
+S FE FL+N++E + L LQ +LL L +NI A A++IK + S
Sbjct: 250 LSCEFEYMSFLENIREGSNPQVLFHLQNQLLGDILEGEGSQNIS-SVAHRASMIKDILLS 309
Query: 318 IKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEE 377
+ IVLDDV+ LSQL+ L G +W G GSRVIITTR++H+L ++ Y+VEGLN EE
Sbjct: 310 RRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQEVDDLYEVEGLNFEE 369
Query: 378 ALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLK 437
A +LFS AF N PK Y +L+ +VV Y LPLA++VLGS L K++ WE ++KL
Sbjct: 370 ACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLD 429
Query: 438 EVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILE 497
I ++LK SYD L+ ++ IFLDLACFFK + + + +L F A G+ L
Sbjct: 430 SEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISNLN 489
Query: 498 EKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIK 557
+ LIT P+ +I MHDLIQ+MG EIVR FP P K SRLW D AL+ D+G ++++
Sbjct: 490 DLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVE 549
Query: 558 GIVMDLSEEGESQLNAKAFSAMTNLKMLKI----------------------------NN 617
+ +DLS+ N+ F+ MT L++LK+ +
Sbjct: 550 TMSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASK 609
Query: 618 VYLSGDLEYLSDQLRFLNWHGYPLE-VHVNY---QLSYVFLVISYYCLKYR----FDKLK 677
+ L ++ S +LR+L W GYPL+ + +N+ +L + L S ++ ++LK
Sbjct: 610 MQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERLK 669
Query: 678 AINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDI 737
I+LS S+ +S+ +F +PNLERL LSGC L +H S+G +K L L L++C LK++
Sbjct: 670 VIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNKLKNL 729
Query: 738 PFNI-SLESLVILTLSGCSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLL 797
P +I LESL L LS CS+ + FP+ GNMK+L E L T+I++L SIG L L L
Sbjct: 730 PDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLESL 789
Query: 798 NLENC-----------------------TNLLELPNTIGSLISLKTLTLHGCSKLDRIPE 857
L NC T + +LP++IG L SL+ L L C+K ++ PE
Sbjct: 790 YLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLEILNLSDCAKFEKFPE 849
Query: 858 SLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPSWNYSSH---- 909
G + L++LD+ T I P S+ L +L+ L S+ FP +
Sbjct: 850 KGGNMKSLKELDLQNTAIKDLPDSIGDLKSLKYLSLSDCSK---FEKFPEKGGNMKRLLQ 909
BLAST of Lsi08G001180 vs. ExPASy Swiss-Prot
Match:
A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)
HSP 1 Score: 484.6 bits (1246), Expect = 4.5e-135
Identity = 329/930 (35.38%), Postives = 510/930 (54.84%), Query Frame = 0
Query: 34 QNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAEKMKAVEESRSS 93
++YDVFLS R +DT ++F L AL +GI F DD + G +E MKA+ ESR +
Sbjct: 10 RSYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFA 69
Query: 94 IVVFSENYGNLA-CMKEVGKIVMCKELRDQLVLPIFYQIDPANVRKQKGNFEKHFNEHEA 153
+VVFS+NY + C++E+ KI+ E + +V+P+FY +DP+ VRKQ G + F + EA
Sbjct: 70 VVVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEA 129
Query: 154 NLGIDNEEVQNWRYSMNQLGHLSGWHLQ-------------------------------- 213
NL D ++V WR ++ ++ ++SG L+
Sbjct: 130 NLVDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNRD 189
Query: 214 ----DSQLHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFLDNVK 273
+SQ+ +++ LL + L +R VGIWGMGG+GKTT AR ++ FE FL++VK
Sbjct: 190 LVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNFESACFLEDVK 249
Query: 274 EALKNEGLASLQEKLLTGALMKRNIEIPNADG-ATLIKRRISSIKALIVLDDVNHLSQLQ 333
E L++ L LQ+ LL+ L ++ + + ++KRR+ S K L+VLDDVNH QL
Sbjct: 250 EYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHNDQLD 309
Query: 334 ELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTNHPKK 393
+L G DWFG GSR++ITTRD LL +H + Y+++ L +EA++LF+ AF + P+K
Sbjct: 310 KLVGAEDWFGSGSRIVITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPEK 369
Query: 394 GYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKISYDL 453
+ +L VV+Y G LPLA++VLGS L + +DVW + +++LK+ + +I LKIS+D
Sbjct: 370 EFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLKDNPEGEIMATLKISFDG 429
Query: 454 LEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTPHEKIQMHDL 513
L + E+ IFLD+ACFF+ +++ + + GF ++G++ L EKSLI +KIQMHDL
Sbjct: 430 LRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILEDKIQMHDL 489
Query: 514 IQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVM---DLSEEGESQ- 573
+QEMG++I + P R++ +DV A D EAI+G+++ + EEGE +
Sbjct: 490 MQEMGRQIAVQESP-----MRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEEGELEY 549
Query: 574 -LNAKAFSAMTNLKMLKINNVYLSG---DLEYLSDQLRFLNWHGYPL-EVHVNYQLS-YV 633
+A+A L++L + Y G + YL + L +L W Y N++ S V
Sbjct: 550 MYSAEALKKTRRLRIL-VKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEPSKLV 609
Query: 634 FL------VISYYCLKYRFDKLKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLH 693
+L +I + R L ++LS + +TPDF + NLERLILS C L ++H
Sbjct: 610 YLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALVEVH 669
Query: 694 QSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKFFPKIVGNMKNLIEFH 753
S+G LK+LILL++ +C L+ +P I E L +L L+ C LK FP++ NM +L +
Sbjct: 670 PSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPEVERNMTHLKKLD 729
Query: 754 LDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGCSKLDRIPES 813
L T I+EL SI L+ L L + +C L+ LP++I +LK + C KL +PE
Sbjct: 730 LTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSIWRFRNLK---ISECEKLGSLPEI 789
Query: 814 LGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPS-WNYSSHYSSQ 873
G C +L + I + P S+ LT+L L+ + K I SL S W +S +
Sbjct: 790 HGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEI--CNCKTISSLSSSIWGLTSLTT-- 849
Query: 874 GLKLTYCFS-----------SFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSG-NN 897
LKL C + S L L L+ + P + L ++I+D+S +
Sbjct: 850 -LKLLDCRKLKNLPGIPNAINHLSGHGLQL----LLTLEQPTIYERLDLLRIIDMSWCSC 909
BLAST of Lsi08G001180 vs. ExPASy Swiss-Prot
Match:
Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)
HSP 1 Score: 464.5 bits (1194), Expect = 4.8e-129
Identity = 353/1069 (33.02%), Postives = 542/1069 (50.70%), Query Frame = 0
Query: 31 PPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAEKMKAVEES 90
P +DVFLS R DT +FT L +AL +GI F D D G L +E+S
Sbjct: 6 PSSAEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID--DRLRRGDNLTALFDRIEKS 65
Query: 91 RSSIVVFSENYGNLA-CMKEVGKIVMCKELRDQLVLPIFYQIDPANVRKQKGNFEKHFNE 150
+ +I+VFS NY N A C++E+ KI+ C+ QLV+PIFY++D ++V KQ+ +F F
Sbjct: 66 KIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKL 125
Query: 151 HEANL-GIDNEEVQNWRYSMNQLGHLSGWHLQ---------------------------- 210
E G+ EE+ +W+ ++ ++ G+ ++
Sbjct: 126 PELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAPSG 185
Query: 211 -------DSQLHQVNRLLG-IGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFL 270
+S+L + +LL LD + +GI GM GIGKTTLA +Y + F+G FL
Sbjct: 186 NEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSCFL 245
Query: 271 DNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGA-TLIKRRISSIKALIVLDDVNHL 330
N++E GL SL +KL + L R++EI A +RR+ S + LIVLDDVN
Sbjct: 246 TNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVNDE 305
Query: 331 SQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTN 390
Q++ L G W+ GSR+IITTRD L+ + R+Y + LN EAL+LFS AF +
Sbjct: 306 KQIRYLMGHCKWYQGGSRIIITTRDSKLIETIK-GRKYVLPKLNDREALKLFSLNAFSNS 365
Query: 391 HPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKI 450
P K + L+ V++Y PLA++VLGS L ++ WE +++LK I+E+L+
Sbjct: 366 FPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLET 425
Query: 451 SYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTPHEKIQ 510
SY+ L ++ +FLD+ACFF+ ++ +L S G ++ L +K LIT +I+
Sbjct: 426 SYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITLSDNRIE 485
Query: 511 MHDLIQEMGQEIVRRM-------------FPNNPEKRSRLWLRKDVNLALSHDQGTEAIK 570
MHD++Q M +EI ++ N + RLW +D+ L+ GT+ I+
Sbjct: 486 MHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKIR 545
Query: 571 GIVMDLSEEGESQLNAKAFSAMTNLKMLKINN------------VYLSGDLEYLSDQLRF 630
GI +D S+ +L+AKAF M NLK LKI + ++L L +L ++L +
Sbjct: 546 GIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNELTY 605
Query: 631 LNWHGYPLE-VHVNYQ-LSYVFLVISYYCLKYRFD------KLKAINLSDSQFISKTPDF 690
L+WHGYPL+ + +++ + V L + + L+ +D LK ++LS S + +
Sbjct: 606 LHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCLGL 665
Query: 691 FGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSG 750
NLERL L GC L KL ++ L+ LI L+L++C L+ +P I +SL L LSG
Sbjct: 666 ANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLILSG 725
Query: 751 CSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGS 810
CS LK FP I N++ L+ LDGT I+ L SI L LLNL+NC L L + +
Sbjct: 726 CSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLYK 785
Query: 811 LISLKTLTLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLS 870
L L+ L L GCS+L+ PE + LE L + T I + P + L+N++ G S
Sbjct: 786 LKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMP-KMMHLSNIKTFSLCGTS 845
Query: 871 RKFIHSLF---PSWNYSSHYSSQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLP 930
S+F P+ S +LT + S CS+ KL P+N+ L
Sbjct: 846 SHVSVSMFFMPPTLGCS--------RLTDLYLSRCSLYKL------------PDNIGGLS 905
Query: 931 SVQILDLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEY 990
S+Q L LSGNN LP+S QL +L+ L CK L+ LP LP +++ ++A +C SL+
Sbjct: 906 SLQSLCLSGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETL 965
Query: 991 YNQEKQMPSSST--GMAVIS-CPITNEEKNFKI---DRVNLSSIHLRTMVQRYIEVLTWQ 1019
N + M + S C N++ + R+ S + +RY
Sbjct: 966 ANPLTPLTVGERIHSMFIFSNCYKLNQDAQASLVGHARIK-SQLMANASAKRYYRGFV-P 1025
BLAST of Lsi08G001180 vs. ExPASy TrEMBL
Match:
A0A5A7T7U3 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001130 PE=4 SV=1)
HSP 1 Score: 2617.8 bits (6784), Expect = 0.0e+00
Identity = 1331/1699 (78.34%), Postives = 1468/1699 (86.40%), Query Frame = 0
Query: 8 MERGASITSLSSPPSPPSLSLALPPLQNYDVFLSHRAK------DTGRSFTADLHEALAS 67
MER SITSLSSPP S+SL LPPL+ YDVFLSHRAK DTGRSF +DLHEAL S
Sbjct: 7 MERRDSITSLSSPPY--SISLPLPPLRRYDVFLSHRAKDHRANNDTGRSFISDLHEALTS 66
Query: 68 QGIVVFRDDVDEDDGGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRD 127
QGIVVF D DE+DG KPL EKMKAV+ESRSSIVVFSENYG+ CMKE+ KI MC++LRD
Sbjct: 67 QGIVVFIDKEDEEDGWKPLTEKMKAVDESRSSIVVFSENYGSWVCMKEIRKIRMCQKLRD 126
Query: 128 QLVLPIFYQIDPANVRKQKG-NFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQ 187
QLVLPIFY++DP +VRKQ+G + K FNEHEAN I EEV+ WR SMN++G+LSGWHLQ
Sbjct: 127 QLVLPIFYKVDPGDVRKQEGESLVKFFNEHEANPNISIEEVKKWRKSMNKVGNLSGWHLQ 186
Query: 188 DS----------------------------------QLHQVNRLLGIGLDDIRFVGIWGM 247
DS +LHQ+N LLGIGLDDIRFVGIWGM
Sbjct: 187 DSHSEEGAIKEIVNHVFNKLRPDLFRYDDKLVGISQRLHQINMLLGIGLDDIRFVGIWGM 246
Query: 248 GGIGKTTLARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNA 307
GGIGKTTLARIIY+SVSHLF+GCYFLDNVKEALK +G+ASLQEKLLTGALMKRNI+IPNA
Sbjct: 247 GGIGKTTLARIIYRSVSHLFDGCYFLDNVKEALKKQGIASLQEKLLTGALMKRNIDIPNA 306
Query: 308 DGATLIKRRISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIE 367
DGATLIKRRIS+IKALI+LDDV+HLSQLQ+LAG DWFG GSR+I+TTR+EHLLVSHGIE
Sbjct: 307 DGATLIKRRISNIKALIILDDVDHLSQLQQLAGSSDWFGSGSRIIVTTRNEHLLVSHGIE 366
Query: 368 RRYKVEGLNVEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKS 427
+RYKVEGLNVEEALQLFSQKAFGTN+PKK YFDLSIQVVEY GDLPLAIEVLGSSLRDKS
Sbjct: 367 KRYKVEGLNVEEALQLFSQKAFGTNYPKKDYFDLSIQVVEYSGDLPLAIEVLGSSLRDKS 426
Query: 428 MDVWENAVEKLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSF 487
+VWENAVEKLKE+ DKKI EIL++SYDLL++ E+EIFLDLACFFKKKSK+QAIEVLQSF
Sbjct: 427 REVWENAVEKLKEILDKKILEILRVSYDLLDKSEKEIFLDLACFFKKKSKKQAIEVLQSF 486
Query: 488 GFAAIIGLEILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNL 547
GF AIIGLEILEE+SLITTPHEKIQMHDLIQEMGQE+VRRMFPNNPEKR+RLWLR+DVNL
Sbjct: 487 GFQAIIGLEILEERSLITTPHEKIQMHDLIQEMGQEVVRRMFPNNPEKRTRLWLREDVNL 546
Query: 548 ALSHDQGTEAIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRF 607
ALSHDQG EAI+GIVMD SEEGES LNAK FS MTNL++LKINNV L G+L+YLSDQLRF
Sbjct: 547 ALSHDQGAEAIEGIVMDSSEEGESHLNAKVFSTMTNLRILKINNVSLCGELDYLSDQLRF 606
Query: 608 LNWHGYPLE-VHVNYQLSYVF-------LVISYYCLKYRFDKLKAINLSDSQFISKTPDF 667
L+WHGYP + + N+ + + + R D+LK +NLSDSQFISKTPDF
Sbjct: 607 LSWHGYPSKYLPPNFHPKSILELELPNSFIHHLWKGSKRLDRLKTVNLSDSQFISKTPDF 666
Query: 668 FGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSG 727
GVPNLERLILSGC RL KLHQSLG+LKHLI LDLKNCK LK IPF+ISLESL++L+LS
Sbjct: 667 SGVPNLERLILSGCVRLTKLHQSLGSLKHLIQLDLKNCKALKAIPFSISLESLIVLSLSN 726
Query: 728 CSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGS 787
CS LK FP IVGNMKNL E HLDGTSIQELHPSIG LTGLVLLNLENCTNLLELPNTIGS
Sbjct: 727 CSSLKNFPNIVGNMKNLTELHLDGTSIQELHPSIGHLTGLVLLNLENCTNLLELPNTIGS 786
Query: 788 LISLKTLTLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLS 847
LI LKTLTLHGCSKL RIPESLG IA LEKLDVT TCINQAPLSLQLLTNLEILDC+GLS
Sbjct: 787 LICLKTLTLHGCSKLTRIPESLGFIASLEKLDVTNTCINQAPLSLQLLTNLEILDCRGLS 846
Query: 848 RKFIHSLFPSWNYSSHYSSQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQ 907
RKFIHSLFPSWN SS+ S GLKLTYC SSFCSM+KLNLSDC L DGDIP+NLQSLPS++
Sbjct: 847 RKFIHSLFPSWNSSSYSSQLGLKLTYCLSSFCSMKKLNLSDCSLKDGDIPDNLQSLPSLE 906
Query: 908 ILDLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQ 967
ILDLSGN+F+ LPKS+ LV+LRTL LVNCKRLQELPKLPLSVR+VEARDCVSLKEYYNQ
Sbjct: 907 ILDLSGNSFSFLPKSVEHLVNLRTLYLVNCKRLQELPKLPLSVRSVEARDCVSLKEYYNQ 966
Query: 968 EKQMPSSSTGMAVISCPITNEEKNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIP 1027
EKQMPSSSTGMAVISCPIT+EE NFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIP
Sbjct: 967 EKQMPSSSTGMAVISCPITDEEHNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIP 1026
Query: 1028 YTQLISCFDQRKLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNPQSVGLSE-TFC 1087
YTQLISCFD RKLGSSITVHCH++T E S DNERIGIALSAFFQV +NPQS+G SE TFC
Sbjct: 1027 YTQLISCFDHRKLGSSITVHCHQNTYE-SRDNERIGIALSAFFQVQENPQSIGHSETTFC 1086
Query: 1088 NFIIKLETDNCPLKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTN 1147
NFII LETD+CPLKSP+IFN+NED+L PP+GLLVF+IPFR+ISYWL+QSCCVDISI+PTN
Sbjct: 1087 NFIINLETDDCPLKSPLIFNKNEDELRPPRGLLVFFIPFRIISYWLDQSCCVDISIIPTN 1146
Query: 1148 PMVKVKACGVSLLFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGG 1207
PMVKVKACGVSLLFQ+NGG+FIGKIMKGLFGSPDF HKFM++HILN+QNHVDVSSLVEGG
Sbjct: 1147 PMVKVKACGVSLLFQQNGGVFIGKIMKGLFGSPDFAHKFMLEHILNQQNHVDVSSLVEGG 1206
Query: 1208 ANARSYWLNALHRTVGTL-PKLRPSVQSNDIEGCSSSNVAIEQVLSEND-STIMLKRNLK 1267
NARSYWLNALHRTVG L PKL+PS+QSNDIE SSSN+AIEQV ++ND TIML+RNLK
Sbjct: 1207 PNARSYWLNALHRTVGVLPPKLQPSIQSNDIEDGSSSNLAIEQVSTQNDHPTIMLQRNLK 1266
Query: 1268 S-------ELKLNGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAI 1327
S ELKLNGEYY FPRGEISKRWF LQVK+ SVTI+VPP LHKNKKWMGL FFAI
Sbjct: 1267 SVLRRIFEELKLNGEYYCFPRGEISKRWFTLQVKRPSVTIKVPPNLHKNKKWMGLAFFAI 1326
Query: 1328 FGSDNKSNISQSFKYQLECDKYHLGRPSVIRLHDGAF-NDSHQLWVSYEPRAVYPYSLNN 1387
F SD SNISQSF YQLE D+Y LGRPS+IRLHDGAF NDS QLWVS+EPR VYPY LN
Sbjct: 1327 FASDINSNISQSFSYQLEFDEYPLGRPSIIRLHDGAFSNDSRQLWVSFEPREVYPYRLNK 1386
Query: 1388 WRHVRVSFLPNCTQTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGM 1447
WR++RVSFLP+C+QTKVILCGARLLY++DL+EFV IIDSVLGCS+NLHEFY+GVF M
Sbjct: 1387 WRNLRVSFLPSCSQTKVILCGARLLYQEDLDEFVDTIIDSVLGCSINLHEFYDGVFLNSM 1446
Query: 1448 MSLIRSQKYDPNIEDEDDEEE----IETRGENYASTSSSSLVPTTKS-LDDSNDYYFELK 1507
+SLIRSQKYDP+IE+E+++EE +ETRG NYASTSSSSLV TTK LDDSNDYY++LK
Sbjct: 1447 LSLIRSQKYDPDIEEEEEDEEDEALMETRGGNYASTSSSSLVSTTKGRLDDSNDYYYDLK 1506
Query: 1508 KCLHCSGELTRTSTNLTGQPTRSYNRQVVDLPNRYDTAFDFIIRGHDVPQLFSRQPERNR 1567
+C H + + L NRYDTAFDFI+RGHDVPQLFSRQPERNR
Sbjct: 1507 QCFHVFFQRS--------------------LQNRYDTAFDFIVRGHDVPQLFSRQPERNR 1566
Query: 1568 ASIQLPPTLYTNNDWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLH 1627
ASI+LPPTLYT+N WIGF+VCTLLY+NKNPTAI+NNLGSRMPHDLMCQFEIEQGLLKPLH
Sbjct: 1567 ASIELPPTLYTSNVWIGFVVCTLLYVNKNPTAIYNNLGSRMPHDLMCQFEIEQGLLKPLH 1626
Query: 1628 IHTTMENKWLWLDERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVH 1642
IHTTMENKWLWLDERQFVWLYYTPRRTFGNILR CSY+R VEADSPEL VRRCGIYL+H
Sbjct: 1627 IHTTMENKWLWLDERQFVWLYYTPRRTFGNILRHCSYIRTIVEADSPELTVRRCGIYLLH 1682
BLAST of Lsi08G001180 vs. ExPASy TrEMBL
Match:
A0A1S3CAF1 (LOW QUALITY PROTEIN: TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498652 PE=4 SV=1)
HSP 1 Score: 2392.8 bits (6200), Expect = 0.0e+00
Identity = 1202/1481 (81.16%), Postives = 1323/1481 (89.33%), Query Frame = 0
Query: 183 QLHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFLDNVKEALKNE 242
+LHQ+N LLGIGLDDIRFVGIWGMGGIGKTTLARIIY+SVSHLF+GCYFLDNVKEALK +
Sbjct: 46 RLHQINMLLGIGLDDIRFVGIWGMGGIGKTTLARIIYRSVSHLFDGCYFLDNVKEALKKQ 105
Query: 243 GLASLQEKLLTGALMKRNIEIPNADGATLIKRRISSIKALIVLDDVNHLSQLQELAGGYD 302
G+ASLQEKLLTGALMKRNI+IPNADGATLIKRRIS+IKALI+LDDV+HLSQLQ+LAG D
Sbjct: 106 GIASLQEKLLTGALMKRNIDIPNADGATLIKRRISNIKALIILDDVDHLSQLQQLAGSSD 165
Query: 303 WFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTNHPKKGYFDLSI 362
WFG GSR+I+TTR+EHLLVSHGIE+RYKVEGLNVEEALQLFSQKAFGTN+PKK YFDLSI
Sbjct: 166 WFGSGSRIIVTTRNEHLLVSHGIEKRYKVEGLNVEEALQLFSQKAFGTNYPKKDYFDLSI 225
Query: 363 QVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKISYDLLEEPEQE 422
QVVEY GDLPLAIEVLGSSLRDKS +VW+NAVEKLKE+ DKKI EIL++SYDLL++ E+E
Sbjct: 226 QVVEYSGDLPLAIEVLGSSLRDKSREVWKNAVEKLKEIRDKKILEILRVSYDLLDKSEKE 285
Query: 423 IFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTPHEKIQMHDLIQEMGQE 482
IFLDLACFFKKKSK+QAIEVLQSFGF AIIGLEILEE+SLITTPHEKIQMHDLIQEMGQE
Sbjct: 286 IFLDLACFFKKKSKKQAIEVLQSFGFQAIIGLEILEERSLITTPHEKIQMHDLIQEMGQE 345
Query: 483 IVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVMDLSEEGESQLNAKAFSAMTN 542
+VRRMFPNNPEKR+RLWLR+DVNLALSHDQG EAI+GIVMD SEEGES LNAK FS MTN
Sbjct: 346 VVRRMFPNNPEKRTRLWLREDVNLALSHDQGAEAIEGIVMDSSEEGESHLNAKVFSTMTN 405
Query: 543 LKMLKINNVYLSGDLEYLSDQLRFLNWHGYPLE-VHVNYQ-LSYVFLVISYYCLKY---- 602
L++LKINNV L G+L+YLSDQLRFL+WHGYP + + N+ S + L + + Y
Sbjct: 406 LRILKINNVSLCGELDYLSDQLRFLSWHGYPSKYLPPNFHPKSILELELPNSFIHYLWKG 465
Query: 603 --RFDKLKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLK 662
R D+LK +NLSDSQFISKTPDF GVPNLERLILSGC RL KLHQSLG+LK LI LDLK
Sbjct: 466 SKRLDRLKTVNLSDSQFISKTPDFSGVPNLERLILSGCVRLTKLHQSLGSLKRLIQLDLK 525
Query: 663 NCKCLKDIPFNISLESLVILTLSGCSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQ 722
NCK LK IPF+ISLESL++L+LS CS LK FP IVGNMKNL E HLDGTSIQELHPSIG
Sbjct: 526 NCKALKAIPFSISLESLIVLSLSNCSSLKNFPNIVGNMKNLTELHLDGTSIQELHPSIGH 585
Query: 723 LTGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGCSKLDRIPESLGTIACLEKLDVTGT 782
LT LVLLNLENCTNLLELPNTIGSLI LKTLTLHGCSKL RIPESLG IA LEKLDVT T
Sbjct: 586 LTRLVLLNLENCTNLLELPNTIGSLICLKTLTLHGCSKLTRIPESLGFIASLEKLDVTNT 645
Query: 783 CINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPSWNYSSHYSSQGLKLTYCFSSFCSMRK 842
CINQAPLSLQLLTNLEILDC+GLSRKFIHSLFPSWN SS+ S GLKLTYC SSFCSM+K
Sbjct: 646 CINQAPLSLQLLTNLEILDCRGLSRKFIHSLFPSWNSSSYSSXLGLKLTYCLSSFCSMKK 705
Query: 843 LNLSDCKLMDGDIPNNLQSLPSVQILDLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQEL 902
LNLSDC L DGDIP+NLQSLPS++ILDLSGN+F+ LPKS+ LV+LRTL LVNCKRLQEL
Sbjct: 706 LNLSDCSLKDGDIPDNLQSLPSLEILDLSGNSFSFLPKSVEHLVNLRTLYLVNCKRLQEL 765
Query: 903 PKLPLSVRNVEARDCVSLKEYYNQEKQMPSSSTGMAVISCPITNEEKNFKIDRVNLSSIH 962
PKLPLSVR+VEARDCVSLKEYYNQEKQMPSSSTGMAVISCPIT+EE NFKIDRVNLSSIH
Sbjct: 766 PKLPLSVRSVEARDCVSLKEYYNQEKQMPSSSTGMAVISCPITDEEHNFKIDRVNLSSIH 825
Query: 963 LRTMVQRYIEVLTWQQEEYFFTIPYTQLISCFDQRKLGSSITVHCHEDTCEISEDNERIG 1022
LRTMVQRYIEVLTWQQEEYFFTIPYTQLISCFD RKLGSSITVHCH++T E S DNERIG
Sbjct: 826 LRTMVQRYIEVLTWQQEEYFFTIPYTQLISCFDHRKLGSSITVHCHQNTYE-SRDNERIG 885
Query: 1023 IALSAFFQVLQNPQSVGLSE-TFCNFIIKLETDNCPLKSPIIFNRNEDKLHPPQGLLVFY 1082
IALSAFFQV +NPQ++G SE TFCNFII LETD+CPLKSP+IFN+NEDKL PP+GLLVF+
Sbjct: 886 IALSAFFQVQENPQNIGHSETTFCNFIINLETDDCPLKSPLIFNKNEDKLRPPRGLLVFF 945
Query: 1083 IPFRMISYWLNQSCCVDISIMPTNPMVKVKACGVSLLFQKNGGMFIGKIMKGLFGSPDFV 1142
IPFR+ISYWL+QSCCVDISI+PTNPMVKVKACGVSLLFQ+NGG+FIGKIMKGLFGSPDF
Sbjct: 946 IPFRIISYWLDQSCCVDISIIPTNPMVKVKACGVSLLFQQNGGVFIGKIMKGLFGSPDFA 1005
Query: 1143 HKFMVDHILNRQNHVDVSSLVEGGANARSYWLNALHRTVGTL-PKLRPSVQSNDIEGCSS 1202
HKFM++HILN+QNHVDVSSLVEGG NARSYWLNALHRTVG L PKL+PS+QSNDIE SS
Sbjct: 1006 HKFMLEHILNQQNHVDVSSLVEGGPNARSYWLNALHRTVGVLPPKLQPSIQSNDIEDGSS 1065
Query: 1203 SNVAIEQVLSEND-STIMLKRNLKS-------ELKLNGEYYIFPRGEISKRWFNLQVKKA 1262
SN+AIEQV ++ND TIMLKRNLKS ELKLNGEYY FPRGEISKRWF LQVK+
Sbjct: 1066 SNLAIEQVSTQNDHPTIMLKRNLKSVLRRIFEELKLNGEYYCFPRGEISKRWFTLQVKRP 1125
Query: 1263 SVTIEVPPKLHKNKKWMGLTFFAIFGSDNKSNISQSFKYQLECDKYHLGRPSVIRLHDGA 1322
SVTI+VPP LHKNKKWMGL FFAIF SD SNISQSF YQLE D+Y LGRPS+IRLHDGA
Sbjct: 1126 SVTIKVPPNLHKNKKWMGLAFFAIFASDINSNISQSFSYQLEFDEYPLGRPSIIRLHDGA 1185
Query: 1323 F-NDSHQLWVSYEPRAVYPYSLNNWRHVRVSFLPNCTQTKVILCGARLLYKQDLNEFVQA 1382
F NDS QLWVS+EPR VYPY LN WR++RVSFLP+C+QTKVILCGARLLY++DL+EFV
Sbjct: 1186 FSNDSRQLWVSFEPREVYPYRLNKWRNLRVSFLPSCSQTKVILCGARLLYQEDLDEFVDT 1245
Query: 1383 IIDSVLGCSLNLHEFYNGVFSKGMMSLIRSQKYDPNIEDEDDEEE--IETRGENYASTSS 1442
IIDSVLGCS+NLHEFY+GVF M+SLIRSQKYDP+IE+++D++E +ETRG NYASTSS
Sbjct: 1246 IIDSVLGCSINLHEFYDGVFLNSMLSLIRSQKYDPDIEEDEDKDEALMETRGGNYASTSS 1305
Query: 1443 SSLVPTTKS-LDDSNDYYFELKKCLHCSGELTRTSTNLTGQPTRSYNRQVVDLPNRYDTA 1502
SSL TTK LDDSNDYY++LK+CLH + + L NRYDTA
Sbjct: 1306 SSLESTTKGRLDDSNDYYYDLKQCLHVFFQRS--------------------LQNRYDTA 1365
Query: 1503 FDFIIRGHDVPQLFSRQPERNRASIQLPPTLYTNNDWIGFIVCTLLYINKNPTAIFNNLG 1562
FDFI+RGHDVPQLFSRQPERNRASI+LPPTLYT+N WIGF+VCTLLY+NKNPTAI+NNLG
Sbjct: 1366 FDFIVRGHDVPQLFSRQPERNRASIELPPTLYTSNVWIGFVVCTLLYVNKNPTAIYNNLG 1425
Query: 1563 SRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLDERQFVWLYYTPRRTFGNILRQCSYV 1622
SRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLDERQFVWLYYTPRRTFG+ILR CSY+
Sbjct: 1426 SRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLDERQFVWLYYTPRRTFGHILRHCSYI 1485
Query: 1623 RANVEADSPELAVRRCGIYLVHNQDREKIDQILIESISARN 1642
RA VEADSPEL VRRCGIYL+HNQDREKIDQILIES+ R+
Sbjct: 1486 RAIVEADSPELTVRRCGIYLLHNQDREKIDQILIESLPTRS 1505
BLAST of Lsi08G001180 vs. ExPASy TrEMBL
Match:
A0A1S3CJJ5 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103501686 PE=4 SV=1)
HSP 1 Score: 2055.0 bits (5323), Expect = 0.0e+00
Identity = 1046/1684 (62.11%), Postives = 1272/1684 (75.53%), Query Frame = 0
Query: 10 RGASITSLSSPPSPPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRD 69
R +SI SLSSPP P SLS LPPL+NYDVFLSHRAKDTG SF ADLH+AL SQGIVV+RD
Sbjct: 10 RVSSIASLSSPPPPYSLSFPLPPLRNYDVFLSHRAKDTGCSFAADLHKALTSQGIVVYRD 69
Query: 70 DVDEDDGGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRDQLVLPIFY 129
+E+ GKPL EKMKAVEESR SIV+FSENYGNL CMKE+ KIVMCKEL DQLVLPIFY
Sbjct: 70 HENEEGRGKPLVEKMKAVEESRCSIVIFSENYGNLVCMKEIEKIVMCKELMDQLVLPIFY 129
Query: 130 QIDPANVRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQDSQ------ 189
+IDP NVRKQKGNFEKHFNEHEAN ID EEV++WRYSM Q+GHLSGWH+QDSQ
Sbjct: 130 KIDPTNVRKQKGNFEKHFNEHEANHEIDIEEVESWRYSMKQVGHLSGWHIQDSQSEAGAI 189
Query: 190 ----------------------------LHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLA 249
LHQ+N LLGIGLDD+RFVGIWGMGGIGKTTLA
Sbjct: 190 DEIVKHIFNKLRPDLFRYDDKLVGISPRLHQINMLLGIGLDDVRFVGIWGMGGIGKTTLA 249
Query: 250 RIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGATLIKRR 309
RIIY+SVSHLFEGCYFLDNVKEALK EGLASLQEKLLTGALMKRNI+IPNADGATLIKRR
Sbjct: 250 RIIYRSVSHLFEGCYFLDNVKEALKKEGLASLQEKLLTGALMKRNIDIPNADGATLIKRR 309
Query: 310 ISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLN 369
IS++KALI+LDDVNHLSQLQ+LAGG DWFGPGSRVI+TTRDEHLL+SHGIERRY VEGL
Sbjct: 310 ISNLKALIILDDVNHLSQLQKLAGGSDWFGPGSRVIVTTRDEHLLISHGIERRYNVEGLK 369
Query: 370 VEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVE 429
+EEALQLFSQKAFG +HP+KGYFD+ QVV+Y G LPLAIEV GSSLR+K M+ WENAVE
Sbjct: 370 IEEALQLFSQKAFGKDHPEKGYFDVCSQVVDYCGGLPLAIEVFGSSLRNKPMEQWENAVE 429
Query: 430 KLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLE 489
KLKEV DKKI E LKI Y +LE+ EQ+IFLD+ACFFK+KSK+QAIE+L+SFGF A++GLE
Sbjct: 430 KLKEVCDKKILEKLKIGYYMLEKSEQKIFLDIACFFKRKSKRQAIEILESFGFPAVLGLE 489
Query: 490 ILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTE 549
ILEEKSLIT PH+KIQMHDLIQEMGQEIVR+ FPN PEKRSRLWLR+D+NLALS D+GTE
Sbjct: 490 ILEEKSLITVPHDKIQMHDLIQEMGQEIVRQNFPNEPEKRSRLWLREDINLALSRDEGTE 549
Query: 550 AIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLNWHGYPL- 609
AI+GI+MDL EEGES LNAK+FSAMTNL++LK+NNV+L ++EYLSDQLRF+NWHGYPL
Sbjct: 550 AIEGIMMDLDEEGESHLNAKSFSAMTNLRVLKVNNVHLCEEIEYLSDQLRFINWHGYPLT 609
Query: 610 EVHVNYQLSYVF-------LVISYYCLKYRFDKLKAINLSDSQFISKTPDFFGVPNLERL 669
+ N+ + + + + + + LK INLSDSQF+SKTPD GVP LERL
Sbjct: 610 TLPSNFNPTNLLELELPNSSIQNLWTASKSLETLKVINLSDSQFLSKTPDLSGVPYLERL 669
Query: 670 ILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKFFPK 729
+LSGC L++LH SLG LKHL LDLK+CK L IPFNI LESL LSGCS L FPK
Sbjct: 670 VLSGCVELHQLHHSLGNLKHLTQLDLKHCKKLTSIPFNICLESLNTFVLSGCSNLTHFPK 729
Query: 730 IVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTLTL 789
I NM +L+E HLD TSI+ LH SIG LTGLVLLNL NCTNLL+LP TIG L SLK+L L
Sbjct: 730 ISANMNHLLELHLDETSIKTLHSSIGHLTGLVLLNLRNCTNLLKLPTTIGCLTSLKSLNL 789
Query: 790 HGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFP 849
HGCSKLD +PESLG I+CLEKLD+T TC+NQAP+SLQLLT LEIL+CQGLSRKF+HSLFP
Sbjct: 790 HGCSKLDSLPESLGNISCLEKLDITSTCVNQAPMSLQLLTKLEILNCQGLSRKFLHSLFP 849
Query: 850 SWNYSSHYS-SQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSGNN 909
+WN++ +S SQGLK+T F+ CS+R LNLSDC L DGD+PN+L SL S+Q+LDLS N+
Sbjct: 850 TWNFTRKFSNSQGLKVTIWFNFGCSLRVLNLSDCNLWDGDLPNDLHSLASLQVLDLSQNH 909
Query: 910 FTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQEKQMPSSS 969
FT LP+S+ LV+LR L LV C L LPKLPLSVR+V+ARDCVSLKEYYNQEKQ+PSS
Sbjct: 910 FTKLPESIRHLVNLRGLFLVECFHLLCLPKLPLSVRDVDARDCVSLKEYYNQEKQIPSSE 969
Query: 970 TGMAVISCPITNE-EKNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIPYTQLISC 1029
GM +I CPITNE +++KI + LS+IHLRT QRY+EVLTWQQE+YFF IPY I+C
Sbjct: 970 MGMTIIRCPITNEPTQSYKIHQPALSAIHLRTTTQRYLEVLTWQQEQYFFVIPYPNFIAC 1029
Query: 1030 FDQRKLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNPQSVGLS-ETFCNFIIKLE 1089
FD+++ G SIT HC D +SEDN RIGIAL A F+V ++ S S + C+FI+K+E
Sbjct: 1030 FDEKRYGFSITAHCPPD--YVSEDNPRIGIALGAAFEVQKHEISNNNSPKVCCDFIVKME 1089
Query: 1090 TDNCPLKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTNPMVKVKA 1149
TD CPLKSP++F+ N+D+L GL VFYIP IS WLNQ CC+++SI+ NP VKVK
Sbjct: 1090 TDECPLKSPLVFDGNKDELKSQMGLSVFYIPTNRISRWLNQCCCIEVSIITDNPFVKVKW 1149
Query: 1150 CGVSLLFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGGANARSYW 1209
CG S+L+++N G FIGKI+K LFGSPD H +VDH+LNRQN VDVS+L++GGA ++ W
Sbjct: 1150 CGASILYEQNAGSFIGKIIKALFGSPDKYHTSIVDHLLNRQNRVDVSTLLDGGARYKTSW 1209
Query: 1210 LNALHRTVGTLPKLRPSVQSND-IEGCSSSNVAIEQVLSENDSTIMLKRNLKS------- 1269
NAL RT+G+ P+LRPS Q + + CS+ N E SE+D +IMLKRNL +
Sbjct: 1210 FNALQRTIGSFPRLRPSKQPREAMLDCSTMNATFEGEESESDYSIMLKRNLTATLLRTFE 1269
Query: 1270 ELKLNGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAIFGSDNKSN 1329
ELKL EYYIFP+ E+S+R+FN Q+++ +TI++PP LHK+KKWMGL FF +F D S
Sbjct: 1270 ELKLYAEYYIFPQKEMSRRFFNFQLEEPKITIKIPPNLHKDKKWMGLAFFVVFSVDENSQ 1329
Query: 1330 ISQSFKYQLECDKYHLGRPSVIRLHDGAFNDSHQLWVSYEPRAVYPYSLNNWRHVRVSFL 1389
S SF YQ++ D+Y + R S++ L+ SHQLWV +EPRAVYPY LN WRH+R S +
Sbjct: 1330 KSHSFSYQVDNDEYRMERESMLYLNKDLLVGSHQLWVFFEPRAVYPYRLNQWRHLRFSIV 1389
Query: 1390 PNCTQTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGMMSLIRSQKY 1449
N + K +LCGA L+YKQDL FV I+ +VL LHEFY+ + + ++ + KY
Sbjct: 1390 CNNSDFKAVLCGANLVYKQDLEGFVNIIVSNVLSSPAELHEFYDRSYVESILRNVHCHKY 1449
Query: 1450 DPNIEDEDDEEEIETRGENYASTSSSSLVPTTKSLDDSNDYYFELKKCLHCSGELTRTST 1509
DP + D + R E + S+ P + +DS+ S + R+
Sbjct: 1450 DPKKNENDQRRQDHLRIEKWVEEQDSNAHP--QEDEDSSS-----------SSNMERSHF 1509
Query: 1510 NLTGQPTRSYNRQVVDLPNRYDTAFDFIIRGHDV-PQLFSRQPERNRASIQLPPTLYTNN 1569
+L Q S+ ++ DL +RY+ FDF+I ++ PQL ++ RN IQLPP YTN
Sbjct: 1510 SLLKQSIPSFLQK--DLKDRYEMTFDFVIPRRNIRPQLINQLSPRNYTRIQLPPNSYTNI 1569
Query: 1570 DWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLD 1629
DW+GF V T+ INK+PTAI NNLGS H+L+CQF IE GL+ PLHIH+ +E+K +WL
Sbjct: 1570 DWMGFAVWTVFQINKHPTAILNNLGSVSRHELICQFGIENGLINPLHIHSIIEDKVIWLH 1629
Query: 1630 ERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVHNQDREKIDQILIE 1640
ERQFVWLYY+PR+ +G I R S+V A +EAD+P+L V CG+ +V+ +D ID+IL+E
Sbjct: 1630 ERQFVWLYYSPRKKYGEIFRHRSHVWAIIEADTPDLVVICCGLQVVYKKDVRVIDKILME 1676
BLAST of Lsi08G001180 vs. ExPASy TrEMBL
Match:
A0A6J1EC12 (TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432680 PE=4 SV=1)
HSP 1 Score: 2044.6 bits (5296), Expect = 0.0e+00
Identity = 1060/1697 (62.46%), Postives = 1288/1697 (75.90%), Query Frame = 0
Query: 8 MERGASITSLSSPPSPPSLSLAL---PPLQNYDVFLSHRAKDTGRSFTADLHEALASQGI 67
MER ASI +L SPPSPP LSL+L PP +NYDV++SHR KDTG F ADLH+AL +QGI
Sbjct: 7 MEREASIVAL-SPPSPPPLSLSLLLPPPKRNYDVYISHRFKDTGDGFAADLHKALTAQGI 66
Query: 68 VVFRD--DVDEDDGGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRDQ 127
VVFRD + + ++ GK LAEK+ A+EESRSSIVVFSENYG+L MKE+ KI M KE+RDQ
Sbjct: 67 VVFRDENENENENKGKMLAEKLTAIEESRSSIVVFSENYGDLVSMKELAKIGMYKEVRDQ 126
Query: 128 LVLPIFYQIDPANVRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQD- 187
LVLPIFYQIDPANVRKQKGNFEK F EHE N+G EEVQ+WR SM ++G+LSGWHLQ+
Sbjct: 127 LVLPIFYQIDPANVRKQKGNFEKPFVEHEQNVGF--EEVQSWRDSMFEVGNLSGWHLQEQ 186
Query: 188 ---------------------------------SQLHQVNRLLGIGLDDIRFVGIWGMGG 247
S+LHQVN LLGIGLDD RFVGIWGMGG
Sbjct: 187 QKEEETINEVVKHVFNKLRPDLFRYDDKLVGISSRLHQVNMLLGIGLDDKRFVGIWGMGG 246
Query: 248 IGKTTLARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADG 307
IGKTT+ARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTG LMKRNIEIP+ DG
Sbjct: 247 IGKTTIARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGTLMKRNIEIPDCDG 306
Query: 308 ATLIKRRISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERR 367
ATLIKRRIS++KALI+LDDVNH+SQL++LAG YDWFGPGSRVI+TTRDEHLL+SHGIERR
Sbjct: 307 ATLIKRRISNLKALIILDDVNHISQLEQLAGDYDWFGPGSRVIVTTRDEHLLISHGIERR 366
Query: 368 YKVEGLNVEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMD 427
Y VEGLN++EAL+LFSQKAF +H KKG+FDLS +VV+Y G LPLAIEVLGS+LRDK M
Sbjct: 367 YNVEGLNIDEALELFSQKAFREDHSKKGFFDLSCKVVDYCGGLPLAIEVLGSALRDKPMK 426
Query: 428 VWENAVEKLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGF 487
WENAV KLK+V D +I E LKISY +LE+ EQ+IFLD+ACFFK+KSK+QAIE+LQSFGF
Sbjct: 427 DWENAVAKLKDVRDMEILEKLKISYYMLEKSEQKIFLDIACFFKRKSKRQAIEILQSFGF 486
Query: 488 AAIIGLEILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLAL 547
A++GLE LEEKSLITTPH+KIQMHDLIQEMGQEIVR+ FP+ PEKRSRLWLR+DVNLAL
Sbjct: 487 LAVLGLEKLEEKSLITTPHDKIQMHDLIQEMGQEIVRQNFPDEPEKRSRLWLREDVNLAL 546
Query: 548 SHDQGTEAIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLN 607
S DQGTEAI+GI+MDL EEGES LNA +F AMTNL++LK+NNV+LS DLEYLSDQLRFLN
Sbjct: 547 SRDQGTEAIEGIMMDLDEEGESHLNANSFKAMTNLRVLKLNNVHLSQDLEYLSDQLRFLN 606
Query: 608 WHGYPLE-----VHVNYQLSYVFLVISYYCL---KYRFDKLKAINLSDSQFISKTPDFFG 667
WHGYP + H L S + L RFD LK INLSDS+F+SKTPDF
Sbjct: 607 WHGYPSKFLPSNFHPTNLLELELPSSSIHQLWKDSKRFDTLKVINLSDSKFLSKTPDFSR 666
Query: 668 VPNLERLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCS 727
VPNLERL+LSGC L +LHQSLG+LKHLI LDLK+CK L +IPFNISLESL IL LSGCS
Sbjct: 667 VPNLERLVLSGCVSLYQLHQSLGSLKHLIQLDLKDCKQLSNIPFNISLESLNILVLSGCS 726
Query: 728 RLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLI 787
LK FPKI GNM NL+E HLDGTSI+ LH SIG LTGLV+LNL+NCTNL++LP+TIG L
Sbjct: 727 SLKNFPKISGNMNNLLELHLDGTSIKVLHQSIGHLTGLVILNLKNCTNLVKLPSTIGCLT 786
Query: 788 SLKTLTLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRK 847
SLK L LHGCSK+D IPESLG I+CLEKLDVT TCI QAPLSLQLLTNLEIL+C+ LSRK
Sbjct: 787 SLKILNLHGCSKIDSIPESLGNISCLEKLDVTSTCITQAPLSLQLLTNLEILNCRSLSRK 846
Query: 848 FIHSLFPSWNYSSHYS-SQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQI 907
FI SLFP W+ S +S SQGLKLT CFS CS+R LNLSDC L DGD+P +L+SL S+QI
Sbjct: 847 FIQSLFPCWSLSRKFSNSQGLKLTNCFSFGCSLRVLNLSDCNLWDGDLPMDLRSLSSLQI 906
Query: 908 LDLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQE 967
L L+ N+FT LP+S+ LV+LR L LV C L+ LPKLPLSVR+VEARDCVSL+EYYNQE
Sbjct: 907 LHLNQNHFTILPESISHLVNLRDLFLVECSNLRSLPKLPLSVRDVEARDCVSLEEYYNQE 966
Query: 968 KQMPSSSTGMAVISCPITNEEK-NFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIP 1027
K +PSS G+ I CPI+ E ++KID++ LS+IHLRTM QRYIEVLTWQQE+YFF IP
Sbjct: 967 KHIPSSEMGITFIRCPISTEPAGSYKIDKLGLSAIHLRTMSQRYIEVLTWQQEKYFFLIP 1026
Query: 1028 YTQLISCFDQRKLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNPQSVGLSETFCN 1087
Y I+CFD ++ G SIT HC D ISE+N RIGIAL A F++ QN Q S+ C+
Sbjct: 1027 YPNFIACFDDKRYGCSITAHCPPD--YISEENARIGIALGATFEI-QNNQWNENSKITCD 1086
Query: 1088 FIIKLETDNCPLKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTNP 1147
FII++ETD CPLKS ++F+ N+D+L P GL+VFY+P R I WLNQ CC+D+SIM NP
Sbjct: 1087 FIIRMETDECPLKSALVFDGNKDELQSPVGLVVFYVPMRRIEGWLNQCCCIDVSIMTDNP 1146
Query: 1148 MVKVKACGVSLLFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGGA 1207
VKVK CG S+++++N G FIGKI+KGLFGSP H +VDHILNRQN VDVSSLV GGA
Sbjct: 1147 FVKVKWCGASIIYEQNAGSFIGKIIKGLFGSPGKYHTSIVDHILNRQNRVDVSSLVYGGA 1206
Query: 1208 NARSYWLNALHRTVGTLPKLRPSVQSNDI--EGCSSSNVAIEQVLSENDSTIMLKRNLKS 1267
++ WLNAL RT+G+ P+LR S + +G + A E +E+D +IMLKRNLK+
Sbjct: 1207 RYKTSWLNALQRTIGSFPRLRASKPPPEAIEDGSTGMIAAAEAEETESDYSIMLKRNLKA 1266
Query: 1268 -------ELKLNGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAIF 1327
+LKL GE+Y+FPR EIS+ WFNLQ+KK VTI++PP LHK+KKWMGL FF +F
Sbjct: 1267 MLLRTFEDLKLYGEFYVFPRKEISRSWFNLQLKKPKVTIKIPPNLHKDKKWMGLAFFVVF 1326
Query: 1328 GSDNKSNISQSFKYQLECDKYHLGRPSVIRLHDGAFNDSHQLWVSYEPRAVYPYSLNNWR 1387
G D S + SF YQ+E D+Y + R S++ L G F+DSHQLWV +EPRAVYPY LN WR
Sbjct: 1327 GVDENSPNAHSFSYQVENDEYTMQRESILYLTKGLFDDSHQLWVFFEPRAVYPYRLNQWR 1386
Query: 1388 HVRVSFL-PNCTQTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGMM 1447
H+ VSF+ N + K ++CGARL YK D+ + +I++V+G +LHEFY+ V+ + M+
Sbjct: 1387 HLCVSFVCNNNSSLKAVVCGARLAYKHDVEGLINTMINNVMGSPADLHEFYDQVYVESMI 1446
Query: 1448 SLIRSQKYDP---NIEDEDD---EEEIETRGENYASTSSSSLVPTTKSLDDSNDYYFELK 1507
+I KYDP E EDD EE IE N S T S ++ ELK
Sbjct: 1447 RMIHFHKYDPKQKEAEGEDDLCLEELIEEHNSNGYPQDS-----TLTSNAMERNHLLELK 1506
Query: 1508 KCLHCSGELTRTSTNLTGQPTRSYNRQVVDLPNRYDTAFDFIIRGHDVPQLFSRQPERNR 1567
+ + S+ ++ DL +R+ T FDF+I ++P+ F++Q E+N+
Sbjct: 1507 ETI------------------PSFLQK--DLKDRFGTTFDFVIPRRNIPEWFNQQSEKNQ 1566
Query: 1568 ASIQLPPTLYTNNDWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLH 1627
+IQLPP+LYTN+DW+GF VC L INK+PTAI NNL S H+L+CQF +E G++ P+H
Sbjct: 1567 TAIQLPPSLYTNSDWMGFAVCALFQINKHPTAILNNLRSISRHELLCQFAVENGVIHPIH 1626
Query: 1628 IHTTMENKWLWLDERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVH 1640
IHT E++++WL ERQF+W YY+PR+T+GNILR S++ A +EAD+P++ VR CG+ LV+
Sbjct: 1627 IHTVTEDRFIWLHERQFLWFYYSPRQTYGNILRHRSHIWATIEADTPDMTVRGCGLQLVY 1672
BLAST of Lsi08G001180 vs. ExPASy TrEMBL
Match:
A0A6J1CK08 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012131 PE=4 SV=1)
HSP 1 Score: 2039.6 bits (5283), Expect = 0.0e+00
Identity = 1041/1684 (61.82%), Postives = 1271/1684 (75.48%), Query Frame = 0
Query: 16 SLSSPPSPPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDD 75
SLSSPP P LS PP + +DVFLSHR +DTGR F ADLH+ L QGIVVF+ DD
Sbjct: 12 SLSSPPPPFLLSFPSPPKRTFDVFLSHRVEDTGRGFAADLHDVLTVQGIVVFK-----DD 71
Query: 76 GGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRDQLVLPIFYQIDPAN 135
G +P+ E ++AVEESR+SIVVFSENY + MKE+ KIVMCKE+ +QLVLPIFYQIDPAN
Sbjct: 72 GARPVTEMLRAVEESRASIVVFSENYVDRIRMKELAKIVMCKEMMNQLVLPIFYQIDPAN 131
Query: 136 VRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQDSQ------------ 195
VRKQ+GNFEK F EHE N D +EVQ WR SMNQ+GHLSGWHL+DSQ
Sbjct: 132 VRKQQGNFEKPFCEHEEN---DIKEVQTWRDSMNQVGHLSGWHLKDSQSEAVAIKEVVKH 191
Query: 196 ----------------------LHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKS 255
LHQVN LLGIGLDD+RF+GIWGMGGIGKTT+ARIIYKS
Sbjct: 192 VFNKLRPDLFRYDGKLVGISSRLHQVNMLLGIGLDDVRFIGIWGMGGIGKTTIARIIYKS 251
Query: 256 VSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGATLIKRRISSIKA 315
VSHLFEGCYFLD VKEALK E L SLQEKLL+GALMKRNIEIP+ DGAT IKRRIS++KA
Sbjct: 252 VSHLFEGCYFLDRVKEALKEESLTSLQEKLLSGALMKRNIEIPDGDGATSIKRRISNLKA 311
Query: 316 LIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQ 375
LI++DDVNHLSQLQ+LAGGYDWFGPGSRVI+TTRDEHLL+SHGIERRY VEGLN++E+LQ
Sbjct: 312 LIIIDDVNHLSQLQKLAGGYDWFGPGSRVIVTTRDEHLLISHGIERRYNVEGLNIDESLQ 371
Query: 376 LFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVG 435
LFSQKAF +HPK+GYFDLS QVV+Y G LPLAIEVLGSSLRDK M+ WENAV+KLKEV
Sbjct: 372 LFSQKAFLADHPKEGYFDLSSQVVDYAGGLPLAIEVLGSSLRDKPMEAWENAVDKLKEVR 431
Query: 436 DKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKS 495
D +I E LKISY +LEE EQ+IFLD+ACFFK+KSK+QA+E+LQSFGF A++GLEILEEKS
Sbjct: 432 DMEILEKLKISYYMLEESEQKIFLDIACFFKRKSKRQAVEILQSFGFPAVLGLEILEEKS 491
Query: 496 LITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIV 555
LIT PH+KIQMHDLIQEMGQEIVR+ FPN+PEKRSRLWLR+D+NLALS DQGTEAI+GI+
Sbjct: 492 LITAPHDKIQMHDLIQEMGQEIVRQKFPNDPEKRSRLWLREDINLALSRDQGTEAIEGIM 551
Query: 556 MDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLNWHGYPLEVHVN-- 615
MD SE+GESQLN K+FSAMTNL++LK+NNVYL+G+LEYLSDQLRFLNWHGYPL+ +
Sbjct: 552 MDSSEKGESQLNPKSFSAMTNLRVLKVNNVYLNGELEYLSDQLRFLNWHGYPLKCLPSNF 611
Query: 616 YQLSYVFLVISYYCLKY------RFDKLKAINLSDSQFISKTPDFFGVPNLERLILSGCA 675
+ S + L + C+++ DKLK INLSDSQF+SKTPD GVPNLERLILSGC
Sbjct: 612 HPKSLLELELPCSCIEHLWKGSKSLDKLKVINLSDSQFLSKTPDLSGVPNLERLILSGCV 671
Query: 676 RLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKFFPKIVGNMK 735
RL +LHQSLGTLKHLI LDLK+CK L IPFN+SLESL IL LSGCS LK FPK+ NM
Sbjct: 672 RLLELHQSLGTLKHLIQLDLKDCKQLTTIPFNLSLESLNILVLSGCSSLKNFPKVSANMN 731
Query: 736 NLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGCSKL 795
+L E HLD TSI+ LHPSIG LTGLVLLNL+NC L++LP TIG L SLK L+L GCSKL
Sbjct: 732 HLSELHLDRTSIRILHPSIGHLTGLVLLNLKNCKYLVQLPTTIGCLTSLKILSLRGCSKL 791
Query: 796 DRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPSWNYSS 855
DRIPESLG I+ LEKLD+TGTCINQAP SLQLLT+LEIL+CQGLSR F+HSLFP +S
Sbjct: 792 DRIPESLGNISSLEKLDLTGTCINQAPFSLQLLTSLEILNCQGLSRNFLHSLFPCLGFSR 851
Query: 856 HY--SSQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNN-LQSLPSVQILDLSGNNFTCL 915
+Y SSQGLKLT CFS +R LNLSDC L DGDIPN+ L+ L S++IL LS N+FT L
Sbjct: 852 NYSQSSQGLKLTNCFSFGSCLRVLNLSDCNLWDGDIPNDLLRGLCSLEILHLSQNHFTIL 911
Query: 916 PKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQEKQMPSSSTGMA 975
P+S+ QL +LR L L C LQ LPKLPLSVR+VEARDCVSLKEYYNQEK +PSS GM
Sbjct: 912 PESISQLTNLRDLFLEECGNLQSLPKLPLSVRDVEARDCVSLKEYYNQEKHIPSSEMGMT 971
Query: 976 VISCPITNE-EKNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIPYTQLISCFDQR 1035
I CPI+ E +++K+D++ LS+IHLRTM QRY+EVLTWQQE+Y+F IPY I+CFD +
Sbjct: 972 FIRCPISTEPAESYKVDQLGLSAIHLRTMAQRYLEVLTWQQEKYYFVIPYPNFIACFDDK 1031
Query: 1036 KLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNPQSVGLSETFCNFIIKLETDNCP 1095
+ G SIT HC D SE+N RIGIAL A F+V ++ + S+ C+FII++ETD CP
Sbjct: 1032 RYGFSITAHCSPD--YTSEENPRIGIALGAAFEVQKHQNNNNNSKLSCDFIIRMETDECP 1091
Query: 1096 LKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTNPMVKVKACGVSL 1155
LKS ++ + N D+L P GL+VFYIP IS WLNQ CC+D+SI+ NP+VKVK CG S+
Sbjct: 1092 LKSALVIDGNTDELDSPHGLVVFYIPMTKISEWLNQCCCIDVSIITDNPLVKVKWCGASI 1151
Query: 1156 LFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGGANARSYWLNALH 1215
L+++N G FIG+I+K FGSP H +VDHILNRQ VDVSSL++GGA ++ WLNAL
Sbjct: 1152 LYEQNAGKFIGRIIKSFFGSPGKYHTSIVDHILNRQKRVDVSSLLDGGARYKTCWLNALQ 1211
Query: 1216 RTVGTLPKLRPSVQSND-IEGCS-SSNVAIEQVLSENDSTIMLKRNLKS-------ELKL 1275
RT+G+ P+LRPS + IE CS S+N ++E +E+DS IMLKRNLK+ ELKL
Sbjct: 1212 RTIGSFPRLRPSRPPPEVIEDCSTSTNASVEAQENESDSIIMLKRNLKAVLLRTFEELKL 1271
Query: 1276 NGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAIFGSDNKSNISQS 1335
GEY++FP+ EIS+ WF LQ+KK VTI+VPP LHK+KKWMGL FF +F D KS S S
Sbjct: 1272 YGEYFVFPQKEISRSWFTLQLKKPKVTIKVPPNLHKDKKWMGLAFFVVFAVDEKSTKSHS 1331
Query: 1336 FKYQLECDKYHLGRPSVIRLHDGAFNDSHQLWVSYEPRAVYPYSLNNWRHVRVSFLPNCT 1395
F YQ+E D+Y + R S++ L+ FND HQLW+ YEPRAVYPY LN+WRH+ VSFL N
Sbjct: 1332 FSYQVENDEYTMQRESILYLNKEMFNDYHQLWLFYEPRAVYPYRLNHWRHLCVSFLSNNP 1391
Query: 1396 QTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGMMSLIRSQKYDPNI 1455
K + CGARL+YKQDL F+Q II++VL C +LH FY+ V+ + M+ +I KYDP
Sbjct: 1392 DLKAVACGARLVYKQDLEGFIQMIINNVLSCPPDLHGFYDQVYVEAMLRMIHFHKYDPKE 1451
Query: 1456 EDEDDEEEIETRGENYASTSSSSLVPTTKSLDDSNDYYFELKKCLHCSGELTRTSTNLTG 1515
++E +++ +E ++ L+ + ++ NL G
Sbjct: 1452 KEEQRRQDLCLE-------------------------QWEAEQNLNGHSDQDYSAQNLGG 1511
Query: 1516 QPTRSYNRQV-----VDLPNRYDTAFDFIIRGHDVPQLFSRQPERNRASIQLPPTLYTNN 1575
+ DL +R+ T FDF+I +PQLF++Q +N +I+LPP+LYTN+
Sbjct: 1512 NHILQLKESIPSFLQKDLKDRFGTTFDFVIPRRHIPQLFNQQSTKNYTAIELPPSLYTNS 1571
Query: 1576 DWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLD 1635
+WIGF VCTL +NK+PTAI NNL S H+L+CQF +E GL++P HIHT E+ ++WL
Sbjct: 1572 NWIGFAVCTLFQVNKHPTAILNNLRSASRHELICQFAVENGLIEPFHIHTITEDTFIWLH 1631
Query: 1636 ERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVHNQDREKIDQILIE 1640
ERQFVWLYY+P+ T+GNI R S++ A +EAD+P+L VR CG+ LV+NQD EKID++L+E
Sbjct: 1632 ERQFVWLYYSPKNTYGNIFRHKSHIWAIIEADTPDLTVRCCGLQLVYNQDVEKIDKMLME 1660
BLAST of Lsi08G001180 vs. NCBI nr
Match:
KAA0039320.1 (TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00503.1 TMV resistance protein N-like [Cucumis melo var. makuwa])
HSP 1 Score: 2617.8 bits (6784), Expect = 0.0e+00
Identity = 1331/1699 (78.34%), Postives = 1468/1699 (86.40%), Query Frame = 0
Query: 8 MERGASITSLSSPPSPPSLSLALPPLQNYDVFLSHRAK------DTGRSFTADLHEALAS 67
MER SITSLSSPP S+SL LPPL+ YDVFLSHRAK DTGRSF +DLHEAL S
Sbjct: 7 MERRDSITSLSSPPY--SISLPLPPLRRYDVFLSHRAKDHRANNDTGRSFISDLHEALTS 66
Query: 68 QGIVVFRDDVDEDDGGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRD 127
QGIVVF D DE+DG KPL EKMKAV+ESRSSIVVFSENYG+ CMKE+ KI MC++LRD
Sbjct: 67 QGIVVFIDKEDEEDGWKPLTEKMKAVDESRSSIVVFSENYGSWVCMKEIRKIRMCQKLRD 126
Query: 128 QLVLPIFYQIDPANVRKQKG-NFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQ 187
QLVLPIFY++DP +VRKQ+G + K FNEHEAN I EEV+ WR SMN++G+LSGWHLQ
Sbjct: 127 QLVLPIFYKVDPGDVRKQEGESLVKFFNEHEANPNISIEEVKKWRKSMNKVGNLSGWHLQ 186
Query: 188 DS----------------------------------QLHQVNRLLGIGLDDIRFVGIWGM 247
DS +LHQ+N LLGIGLDDIRFVGIWGM
Sbjct: 187 DSHSEEGAIKEIVNHVFNKLRPDLFRYDDKLVGISQRLHQINMLLGIGLDDIRFVGIWGM 246
Query: 248 GGIGKTTLARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNA 307
GGIGKTTLARIIY+SVSHLF+GCYFLDNVKEALK +G+ASLQEKLLTGALMKRNI+IPNA
Sbjct: 247 GGIGKTTLARIIYRSVSHLFDGCYFLDNVKEALKKQGIASLQEKLLTGALMKRNIDIPNA 306
Query: 308 DGATLIKRRISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIE 367
DGATLIKRRIS+IKALI+LDDV+HLSQLQ+LAG DWFG GSR+I+TTR+EHLLVSHGIE
Sbjct: 307 DGATLIKRRISNIKALIILDDVDHLSQLQQLAGSSDWFGSGSRIIVTTRNEHLLVSHGIE 366
Query: 368 RRYKVEGLNVEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKS 427
+RYKVEGLNVEEALQLFSQKAFGTN+PKK YFDLSIQVVEY GDLPLAIEVLGSSLRDKS
Sbjct: 367 KRYKVEGLNVEEALQLFSQKAFGTNYPKKDYFDLSIQVVEYSGDLPLAIEVLGSSLRDKS 426
Query: 428 MDVWENAVEKLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSF 487
+VWENAVEKLKE+ DKKI EIL++SYDLL++ E+EIFLDLACFFKKKSK+QAIEVLQSF
Sbjct: 427 REVWENAVEKLKEILDKKILEILRVSYDLLDKSEKEIFLDLACFFKKKSKKQAIEVLQSF 486
Query: 488 GFAAIIGLEILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNL 547
GF AIIGLEILEE+SLITTPHEKIQMHDLIQEMGQE+VRRMFPNNPEKR+RLWLR+DVNL
Sbjct: 487 GFQAIIGLEILEERSLITTPHEKIQMHDLIQEMGQEVVRRMFPNNPEKRTRLWLREDVNL 546
Query: 548 ALSHDQGTEAIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRF 607
ALSHDQG EAI+GIVMD SEEGES LNAK FS MTNL++LKINNV L G+L+YLSDQLRF
Sbjct: 547 ALSHDQGAEAIEGIVMDSSEEGESHLNAKVFSTMTNLRILKINNVSLCGELDYLSDQLRF 606
Query: 608 LNWHGYPLE-VHVNYQLSYVF-------LVISYYCLKYRFDKLKAINLSDSQFISKTPDF 667
L+WHGYP + + N+ + + + R D+LK +NLSDSQFISKTPDF
Sbjct: 607 LSWHGYPSKYLPPNFHPKSILELELPNSFIHHLWKGSKRLDRLKTVNLSDSQFISKTPDF 666
Query: 668 FGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSG 727
GVPNLERLILSGC RL KLHQSLG+LKHLI LDLKNCK LK IPF+ISLESL++L+LS
Sbjct: 667 SGVPNLERLILSGCVRLTKLHQSLGSLKHLIQLDLKNCKALKAIPFSISLESLIVLSLSN 726
Query: 728 CSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGS 787
CS LK FP IVGNMKNL E HLDGTSIQELHPSIG LTGLVLLNLENCTNLLELPNTIGS
Sbjct: 727 CSSLKNFPNIVGNMKNLTELHLDGTSIQELHPSIGHLTGLVLLNLENCTNLLELPNTIGS 786
Query: 788 LISLKTLTLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLS 847
LI LKTLTLHGCSKL RIPESLG IA LEKLDVT TCINQAPLSLQLLTNLEILDC+GLS
Sbjct: 787 LICLKTLTLHGCSKLTRIPESLGFIASLEKLDVTNTCINQAPLSLQLLTNLEILDCRGLS 846
Query: 848 RKFIHSLFPSWNYSSHYSSQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQ 907
RKFIHSLFPSWN SS+ S GLKLTYC SSFCSM+KLNLSDC L DGDIP+NLQSLPS++
Sbjct: 847 RKFIHSLFPSWNSSSYSSQLGLKLTYCLSSFCSMKKLNLSDCSLKDGDIPDNLQSLPSLE 906
Query: 908 ILDLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQ 967
ILDLSGN+F+ LPKS+ LV+LRTL LVNCKRLQELPKLPLSVR+VEARDCVSLKEYYNQ
Sbjct: 907 ILDLSGNSFSFLPKSVEHLVNLRTLYLVNCKRLQELPKLPLSVRSVEARDCVSLKEYYNQ 966
Query: 968 EKQMPSSSTGMAVISCPITNEEKNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIP 1027
EKQMPSSSTGMAVISCPIT+EE NFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIP
Sbjct: 967 EKQMPSSSTGMAVISCPITDEEHNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIP 1026
Query: 1028 YTQLISCFDQRKLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNPQSVGLSE-TFC 1087
YTQLISCFD RKLGSSITVHCH++T E S DNERIGIALSAFFQV +NPQS+G SE TFC
Sbjct: 1027 YTQLISCFDHRKLGSSITVHCHQNTYE-SRDNERIGIALSAFFQVQENPQSIGHSETTFC 1086
Query: 1088 NFIIKLETDNCPLKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTN 1147
NFII LETD+CPLKSP+IFN+NED+L PP+GLLVF+IPFR+ISYWL+QSCCVDISI+PTN
Sbjct: 1087 NFIINLETDDCPLKSPLIFNKNEDELRPPRGLLVFFIPFRIISYWLDQSCCVDISIIPTN 1146
Query: 1148 PMVKVKACGVSLLFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGG 1207
PMVKVKACGVSLLFQ+NGG+FIGKIMKGLFGSPDF HKFM++HILN+QNHVDVSSLVEGG
Sbjct: 1147 PMVKVKACGVSLLFQQNGGVFIGKIMKGLFGSPDFAHKFMLEHILNQQNHVDVSSLVEGG 1206
Query: 1208 ANARSYWLNALHRTVGTL-PKLRPSVQSNDIEGCSSSNVAIEQVLSEND-STIMLKRNLK 1267
NARSYWLNALHRTVG L PKL+PS+QSNDIE SSSN+AIEQV ++ND TIML+RNLK
Sbjct: 1207 PNARSYWLNALHRTVGVLPPKLQPSIQSNDIEDGSSSNLAIEQVSTQNDHPTIMLQRNLK 1266
Query: 1268 S-------ELKLNGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAI 1327
S ELKLNGEYY FPRGEISKRWF LQVK+ SVTI+VPP LHKNKKWMGL FFAI
Sbjct: 1267 SVLRRIFEELKLNGEYYCFPRGEISKRWFTLQVKRPSVTIKVPPNLHKNKKWMGLAFFAI 1326
Query: 1328 FGSDNKSNISQSFKYQLECDKYHLGRPSVIRLHDGAF-NDSHQLWVSYEPRAVYPYSLNN 1387
F SD SNISQSF YQLE D+Y LGRPS+IRLHDGAF NDS QLWVS+EPR VYPY LN
Sbjct: 1327 FASDINSNISQSFSYQLEFDEYPLGRPSIIRLHDGAFSNDSRQLWVSFEPREVYPYRLNK 1386
Query: 1388 WRHVRVSFLPNCTQTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGM 1447
WR++RVSFLP+C+QTKVILCGARLLY++DL+EFV IIDSVLGCS+NLHEFY+GVF M
Sbjct: 1387 WRNLRVSFLPSCSQTKVILCGARLLYQEDLDEFVDTIIDSVLGCSINLHEFYDGVFLNSM 1446
Query: 1448 MSLIRSQKYDPNIEDEDDEEE----IETRGENYASTSSSSLVPTTKS-LDDSNDYYFELK 1507
+SLIRSQKYDP+IE+E+++EE +ETRG NYASTSSSSLV TTK LDDSNDYY++LK
Sbjct: 1447 LSLIRSQKYDPDIEEEEEDEEDEALMETRGGNYASTSSSSLVSTTKGRLDDSNDYYYDLK 1506
Query: 1508 KCLHCSGELTRTSTNLTGQPTRSYNRQVVDLPNRYDTAFDFIIRGHDVPQLFSRQPERNR 1567
+C H + + L NRYDTAFDFI+RGHDVPQLFSRQPERNR
Sbjct: 1507 QCFHVFFQRS--------------------LQNRYDTAFDFIVRGHDVPQLFSRQPERNR 1566
Query: 1568 ASIQLPPTLYTNNDWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLH 1627
ASI+LPPTLYT+N WIGF+VCTLLY+NKNPTAI+NNLGSRMPHDLMCQFEIEQGLLKPLH
Sbjct: 1567 ASIELPPTLYTSNVWIGFVVCTLLYVNKNPTAIYNNLGSRMPHDLMCQFEIEQGLLKPLH 1626
Query: 1628 IHTTMENKWLWLDERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVH 1642
IHTTMENKWLWLDERQFVWLYYTPRRTFGNILR CSY+R VEADSPEL VRRCGIYL+H
Sbjct: 1627 IHTTMENKWLWLDERQFVWLYYTPRRTFGNILRHCSYIRTIVEADSPELTVRRCGIYLLH 1682
BLAST of Lsi08G001180 vs. NCBI nr
Match:
XP_008459548.2 (PREDICTED: LOW QUALITY PROTEIN: TMV resistance protein N-like [Cucumis melo])
HSP 1 Score: 2392.8 bits (6200), Expect = 0.0e+00
Identity = 1202/1481 (81.16%), Postives = 1323/1481 (89.33%), Query Frame = 0
Query: 183 QLHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFLDNVKEALKNE 242
+LHQ+N LLGIGLDDIRFVGIWGMGGIGKTTLARIIY+SVSHLF+GCYFLDNVKEALK +
Sbjct: 46 RLHQINMLLGIGLDDIRFVGIWGMGGIGKTTLARIIYRSVSHLFDGCYFLDNVKEALKKQ 105
Query: 243 GLASLQEKLLTGALMKRNIEIPNADGATLIKRRISSIKALIVLDDVNHLSQLQELAGGYD 302
G+ASLQEKLLTGALMKRNI+IPNADGATLIKRRIS+IKALI+LDDV+HLSQLQ+LAG D
Sbjct: 106 GIASLQEKLLTGALMKRNIDIPNADGATLIKRRISNIKALIILDDVDHLSQLQQLAGSSD 165
Query: 303 WFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTNHPKKGYFDLSI 362
WFG GSR+I+TTR+EHLLVSHGIE+RYKVEGLNVEEALQLFSQKAFGTN+PKK YFDLSI
Sbjct: 166 WFGSGSRIIVTTRNEHLLVSHGIEKRYKVEGLNVEEALQLFSQKAFGTNYPKKDYFDLSI 225
Query: 363 QVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKISYDLLEEPEQE 422
QVVEY GDLPLAIEVLGSSLRDKS +VW+NAVEKLKE+ DKKI EIL++SYDLL++ E+E
Sbjct: 226 QVVEYSGDLPLAIEVLGSSLRDKSREVWKNAVEKLKEIRDKKILEILRVSYDLLDKSEKE 285
Query: 423 IFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTPHEKIQMHDLIQEMGQE 482
IFLDLACFFKKKSK+QAIEVLQSFGF AIIGLEILEE+SLITTPHEKIQMHDLIQEMGQE
Sbjct: 286 IFLDLACFFKKKSKKQAIEVLQSFGFQAIIGLEILEERSLITTPHEKIQMHDLIQEMGQE 345
Query: 483 IVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVMDLSEEGESQLNAKAFSAMTN 542
+VRRMFPNNPEKR+RLWLR+DVNLALSHDQG EAI+GIVMD SEEGES LNAK FS MTN
Sbjct: 346 VVRRMFPNNPEKRTRLWLREDVNLALSHDQGAEAIEGIVMDSSEEGESHLNAKVFSTMTN 405
Query: 543 LKMLKINNVYLSGDLEYLSDQLRFLNWHGYPLE-VHVNYQ-LSYVFLVISYYCLKY---- 602
L++LKINNV L G+L+YLSDQLRFL+WHGYP + + N+ S + L + + Y
Sbjct: 406 LRILKINNVSLCGELDYLSDQLRFLSWHGYPSKYLPPNFHPKSILELELPNSFIHYLWKG 465
Query: 603 --RFDKLKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLK 662
R D+LK +NLSDSQFISKTPDF GVPNLERLILSGC RL KLHQSLG+LK LI LDLK
Sbjct: 466 SKRLDRLKTVNLSDSQFISKTPDFSGVPNLERLILSGCVRLTKLHQSLGSLKRLIQLDLK 525
Query: 663 NCKCLKDIPFNISLESLVILTLSGCSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQ 722
NCK LK IPF+ISLESL++L+LS CS LK FP IVGNMKNL E HLDGTSIQELHPSIG
Sbjct: 526 NCKALKAIPFSISLESLIVLSLSNCSSLKNFPNIVGNMKNLTELHLDGTSIQELHPSIGH 585
Query: 723 LTGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGCSKLDRIPESLGTIACLEKLDVTGT 782
LT LVLLNLENCTNLLELPNTIGSLI LKTLTLHGCSKL RIPESLG IA LEKLDVT T
Sbjct: 586 LTRLVLLNLENCTNLLELPNTIGSLICLKTLTLHGCSKLTRIPESLGFIASLEKLDVTNT 645
Query: 783 CINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPSWNYSSHYSSQGLKLTYCFSSFCSMRK 842
CINQAPLSLQLLTNLEILDC+GLSRKFIHSLFPSWN SS+ S GLKLTYC SSFCSM+K
Sbjct: 646 CINQAPLSLQLLTNLEILDCRGLSRKFIHSLFPSWNSSSYSSXLGLKLTYCLSSFCSMKK 705
Query: 843 LNLSDCKLMDGDIPNNLQSLPSVQILDLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQEL 902
LNLSDC L DGDIP+NLQSLPS++ILDLSGN+F+ LPKS+ LV+LRTL LVNCKRLQEL
Sbjct: 706 LNLSDCSLKDGDIPDNLQSLPSLEILDLSGNSFSFLPKSVEHLVNLRTLYLVNCKRLQEL 765
Query: 903 PKLPLSVRNVEARDCVSLKEYYNQEKQMPSSSTGMAVISCPITNEEKNFKIDRVNLSSIH 962
PKLPLSVR+VEARDCVSLKEYYNQEKQMPSSSTGMAVISCPIT+EE NFKIDRVNLSSIH
Sbjct: 766 PKLPLSVRSVEARDCVSLKEYYNQEKQMPSSSTGMAVISCPITDEEHNFKIDRVNLSSIH 825
Query: 963 LRTMVQRYIEVLTWQQEEYFFTIPYTQLISCFDQRKLGSSITVHCHEDTCEISEDNERIG 1022
LRTMVQRYIEVLTWQQEEYFFTIPYTQLISCFD RKLGSSITVHCH++T E S DNERIG
Sbjct: 826 LRTMVQRYIEVLTWQQEEYFFTIPYTQLISCFDHRKLGSSITVHCHQNTYE-SRDNERIG 885
Query: 1023 IALSAFFQVLQNPQSVGLSE-TFCNFIIKLETDNCPLKSPIIFNRNEDKLHPPQGLLVFY 1082
IALSAFFQV +NPQ++G SE TFCNFII LETD+CPLKSP+IFN+NEDKL PP+GLLVF+
Sbjct: 886 IALSAFFQVQENPQNIGHSETTFCNFIINLETDDCPLKSPLIFNKNEDKLRPPRGLLVFF 945
Query: 1083 IPFRMISYWLNQSCCVDISIMPTNPMVKVKACGVSLLFQKNGGMFIGKIMKGLFGSPDFV 1142
IPFR+ISYWL+QSCCVDISI+PTNPMVKVKACGVSLLFQ+NGG+FIGKIMKGLFGSPDF
Sbjct: 946 IPFRIISYWLDQSCCVDISIIPTNPMVKVKACGVSLLFQQNGGVFIGKIMKGLFGSPDFA 1005
Query: 1143 HKFMVDHILNRQNHVDVSSLVEGGANARSYWLNALHRTVGTL-PKLRPSVQSNDIEGCSS 1202
HKFM++HILN+QNHVDVSSLVEGG NARSYWLNALHRTVG L PKL+PS+QSNDIE SS
Sbjct: 1006 HKFMLEHILNQQNHVDVSSLVEGGPNARSYWLNALHRTVGVLPPKLQPSIQSNDIEDGSS 1065
Query: 1203 SNVAIEQVLSEND-STIMLKRNLKS-------ELKLNGEYYIFPRGEISKRWFNLQVKKA 1262
SN+AIEQV ++ND TIMLKRNLKS ELKLNGEYY FPRGEISKRWF LQVK+
Sbjct: 1066 SNLAIEQVSTQNDHPTIMLKRNLKSVLRRIFEELKLNGEYYCFPRGEISKRWFTLQVKRP 1125
Query: 1263 SVTIEVPPKLHKNKKWMGLTFFAIFGSDNKSNISQSFKYQLECDKYHLGRPSVIRLHDGA 1322
SVTI+VPP LHKNKKWMGL FFAIF SD SNISQSF YQLE D+Y LGRPS+IRLHDGA
Sbjct: 1126 SVTIKVPPNLHKNKKWMGLAFFAIFASDINSNISQSFSYQLEFDEYPLGRPSIIRLHDGA 1185
Query: 1323 F-NDSHQLWVSYEPRAVYPYSLNNWRHVRVSFLPNCTQTKVILCGARLLYKQDLNEFVQA 1382
F NDS QLWVS+EPR VYPY LN WR++RVSFLP+C+QTKVILCGARLLY++DL+EFV
Sbjct: 1186 FSNDSRQLWVSFEPREVYPYRLNKWRNLRVSFLPSCSQTKVILCGARLLYQEDLDEFVDT 1245
Query: 1383 IIDSVLGCSLNLHEFYNGVFSKGMMSLIRSQKYDPNIEDEDDEEE--IETRGENYASTSS 1442
IIDSVLGCS+NLHEFY+GVF M+SLIRSQKYDP+IE+++D++E +ETRG NYASTSS
Sbjct: 1246 IIDSVLGCSINLHEFYDGVFLNSMLSLIRSQKYDPDIEEDEDKDEALMETRGGNYASTSS 1305
Query: 1443 SSLVPTTKS-LDDSNDYYFELKKCLHCSGELTRTSTNLTGQPTRSYNRQVVDLPNRYDTA 1502
SSL TTK LDDSNDYY++LK+CLH + + L NRYDTA
Sbjct: 1306 SSLESTTKGRLDDSNDYYYDLKQCLHVFFQRS--------------------LQNRYDTA 1365
Query: 1503 FDFIIRGHDVPQLFSRQPERNRASIQLPPTLYTNNDWIGFIVCTLLYINKNPTAIFNNLG 1562
FDFI+RGHDVPQLFSRQPERNRASI+LPPTLYT+N WIGF+VCTLLY+NKNPTAI+NNLG
Sbjct: 1366 FDFIVRGHDVPQLFSRQPERNRASIELPPTLYTSNVWIGFVVCTLLYVNKNPTAIYNNLG 1425
Query: 1563 SRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLDERQFVWLYYTPRRTFGNILRQCSYV 1622
SRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLDERQFVWLYYTPRRTFG+ILR CSY+
Sbjct: 1426 SRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLDERQFVWLYYTPRRTFGHILRHCSYI 1485
Query: 1623 RANVEADSPELAVRRCGIYLVHNQDREKIDQILIESISARN 1642
RA VEADSPEL VRRCGIYL+HNQDREKIDQILIES+ R+
Sbjct: 1486 RAIVEADSPELTVRRCGIYLLHNQDREKIDQILIESLPTRS 1505
BLAST of Lsi08G001180 vs. NCBI nr
Match:
XP_038890618.1 (TMV resistance protein N-like [Benincasa hispida])
HSP 1 Score: 2088.5 bits (5410), Expect = 0.0e+00
Identity = 1062/1685 (63.03%), Postives = 1287/1685 (76.38%), Query Frame = 0
Query: 8 MERGASITSLSSPPSPPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVF 67
MER ASITSLSSPP S+SL LPPL+NYDVFLSHRAKDTGRSF ADLHEAL +QGIVVF
Sbjct: 7 MERRASITSLSSPPPRYSISLPLPPLRNYDVFLSHRAKDTGRSFAADLHEALTTQGIVVF 66
Query: 68 RDDVDEDDGGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRDQLVLPI 127
RDD DE+DGGKPLAEKMKAVEESRSSIVVFSENYGNL CMKE+ KIVMCKELRDQLVLPI
Sbjct: 67 RDDEDEEDGGKPLAEKMKAVEESRSSIVVFSENYGNLVCMKEIEKIVMCKELRDQLVLPI 126
Query: 128 FYQIDPANVRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQDSQ---- 187
FYQIDPAN RKQKGNFE HFNEHEAN ID EEV++WRYSMNQ+GHLSGWH+QDSQ
Sbjct: 127 FYQIDPANARKQKGNFENHFNEHEANPEIDIEEVESWRYSMNQVGHLSGWHIQDSQSEAG 186
Query: 188 ------------------------------LHQVNRLLGIGLDDIRFVGIWGMGGIGKTT 247
LHQ+N L+GIGLDD+RFVGIWGMGGIGKTT
Sbjct: 187 VINEIVKHIFNKLRPDLFRYDDKFVGISPRLHQINMLMGIGLDDVRFVGIWGMGGIGKTT 246
Query: 248 LARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGATLIK 307
ARIIYKSVSHLFEGCYFLDNVKEALK EGLASLQEKLLTGALMKRNI+IPN DGATLIK
Sbjct: 247 FARIIYKSVSHLFEGCYFLDNVKEALKKEGLASLQEKLLTGALMKRNIDIPNVDGATLIK 306
Query: 308 RRISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEG 367
RRIS++KALI+LDDVNHLSQLQ+L GG DWFG GSRVI+TTRDEHLL+SHGIERRY VEG
Sbjct: 307 RRISNLKALIILDDVNHLSQLQKLVGGLDWFGSGSRVIVTTRDEHLLISHGIERRYNVEG 366
Query: 368 LNVEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENA 427
L +EEALQLFSQKAFG +HPKKGYFDLS QVV Y G LPLAIEVLGSSLRDK ++ WENA
Sbjct: 367 LKIEEALQLFSQKAFGEDHPKKGYFDLSSQVVSYAGGLPLAIEVLGSSLRDKPLEQWENA 426
Query: 428 VEKLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIG 487
VEKLKEV DK+I E LKISY +LEE EQ IFLD+ACFFK+KSK++AI++L+SFGF A++G
Sbjct: 427 VEKLKEVRDKEILEKLKISYYMLEESEQNIFLDIACFFKRKSKRRAIKILESFGFPAVLG 486
Query: 488 LEILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQG 547
LEILEEKSLITTPH+K+QMHDLIQEMGQEIVR+ FPN PEKRSRLWLR+DVNLAL+ D+G
Sbjct: 487 LEILEEKSLITTPHDKLQMHDLIQEMGQEIVRQNFPNKPEKRSRLWLREDVNLALNLDEG 546
Query: 548 TEAIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLNWHGYP 607
TEAI+GIV+D+ EEGES LNAK+FSAMTNL++LK+NNVYLS +L+YLSDQLRFL+WHGYP
Sbjct: 547 TEAIEGIVIDMDEEGESHLNAKSFSAMTNLRVLKLNNVYLSEELQYLSDQLRFLHWHGYP 606
Query: 608 LE-VHVNYQLSYVF-------LVISYYCLKYRFDKLKAINLSDSQFISKTPDFFGVPNLE 667
L+ + N+ + + + + + LK INLSDSQF+SK PDF GVPNLE
Sbjct: 607 LKCLPSNFNPTNLLELELPSSSIQHLWTASKSLETLKVINLSDSQFLSKIPDFSGVPNLE 666
Query: 668 RLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKFF 727
RL+LSGC L++LH SLG LKHLI LDL+NCK L IPFNI LESL IL LSGCS L +F
Sbjct: 667 RLVLSGCVELHQLHPSLGNLKHLIQLDLRNCKKLTTIPFNICLESLHILVLSGCSNLTYF 726
Query: 728 PKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTL 787
PKI GNM +L+E HLD TSI+ LH SIG LT LVLLNL+NCTNLL+LP+TIG L SLKTL
Sbjct: 727 PKISGNMNHLLELHLDETSIKILHSSIGHLTALVLLNLKNCTNLLKLPSTIGCLTSLKTL 786
Query: 788 TLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSL 847
L+GCSKLD +PESLG I+CLEKLD+T TC+NQAP+SLQLLT LEIL+CQGLSRKF+HSL
Sbjct: 787 NLNGCSKLDSLPESLGNISCLEKLDITNTCVNQAPMSLQLLTKLEILNCQGLSRKFLHSL 846
Query: 848 FPSWNYSSHYS-SQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSG 907
FP+WN++ ++ SQGLK+T F+ CS+ LNLSDC L DGD+PN+L SL S+QIL LS
Sbjct: 847 FPTWNFTRKFNHSQGLKVTNWFNFGCSLMVLNLSDCNLWDGDLPNDLHSLASLQILHLSQ 906
Query: 908 NNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQEKQMPS 967
N+FT LP+S+ LVSLR L L C L LPKLPLSVRNVEARDCVSLKEYYNQEKQ+PS
Sbjct: 907 NHFTKLPESISHLVSLRGLFLEECFHLLNLPKLPLSVRNVEARDCVSLKEYYNQEKQIPS 966
Query: 968 SSTGMAVISCPITNE-EKNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIPYTQLI 1027
S GM +I CPI+ E +++KID+ LS+IH+RTM QRYIEVLTWQQ++YFF IPY I
Sbjct: 967 SEMGMTMIRCPISTEPNESYKIDQPRLSAIHIRTMTQRYIEVLTWQQQKYFFVIPYPNFI 1026
Query: 1028 SCFDQRKLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNPQSVGLSETFCNFIIKL 1087
+CFD+++ G SIT HC D ISE N RIGIAL A F+V ++ S S+ C+FI+K+
Sbjct: 1027 ACFDEKRYGFSITAHCPPD--YISEKNPRIGIALGAVFEVQKHEISHDNSKICCDFIVKM 1086
Query: 1088 ETDNCPLKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTNPMVKVK 1147
ETD CPLKS ++F+ N+ +L GL VFYIP + IS WLN+ CC+D+SIM NP VKVK
Sbjct: 1087 ETDECPLKSALVFDGNKAELESQMGLSVFYIPMKRISRWLNECCCIDVSIMTDNPFVKVK 1146
Query: 1148 ACGVSLLFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGGANARSY 1207
CG S+L+++N G FIGKI+K LFGSP H +VDH+LNRQN VDVS+L++GGA ++
Sbjct: 1147 WCGASILYEQNAGSFIGKIIKALFGSPGKYHTSIVDHLLNRQNRVDVSTLLDGGARYKTS 1206
Query: 1208 WLNALHRTVGTLPKLRPSVQSND-IEGCSSSNVAIEQVLSENDSTIMLKRNLKS------ 1267
W NAL RT+G+ +LRPS + IE CS+ N + E +E+D +IMLKRNLK+
Sbjct: 1207 WFNALQRTIGSFSRLRPSRPPREVIEECSTMNASSEAEENESDHSIMLKRNLKATLLRTF 1266
Query: 1268 -ELKLNGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAIFGSDNKS 1327
ELKL GEYY+FP+ E+S+ +FN Q+K+ +TI+VPP LHK KKWMGL FF +F D S
Sbjct: 1267 EELKLYGEYYMFPQKEMSRSFFNFQLKEPKITIKVPPNLHKEKKWMGLAFFVVFSVDESS 1326
Query: 1328 NISQSFKYQLECDKYHLGRPSVIRLHDGAFNDSHQLWVSYEPRAVYPYSLNNWRHVRVSF 1387
+ SF Y ++ D+Y L R S++ L++ DSHQLW+ +EPRAVYPY LN WRH+R S
Sbjct: 1327 PKAHSFSYHVDNDEYRLERESILYLNEDLLVDSHQLWLFFEPRAVYPYRLNQWRHLRFSI 1386
Query: 1388 LPNCTQTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGMMSLIRSQK 1447
+ N + KV+LCGARL+YKQDL FV I+ +VL LHE+Y+ + GM+ + S K
Sbjct: 1387 VCNNSDFKVVLCGARLVYKQDLEGFVNTIVSNVLNSPTELHEYYDQIHVNGMLKNVHSHK 1446
Query: 1448 YDPNIEDEDDEEEIETRGENYASTSSSSLVPTTKSLDDSNDYYFELKKCLHCSGELTRTS 1507
YDP ++ + ++ E + S+ P + CS ++ R+
Sbjct: 1447 YDPKKKENESRQDFPI--EEWEGEQKSNAHPQEED----------------CS-KMERSH 1506
Query: 1508 TNLTGQPTRSYNRQVVDLPNRYDTAFDFIIRGHDV-PQLFSRQPERNRASIQLPPTLYTN 1567
+ S+ ++ DL +R++T FDF+I +V PQL ++ ++ SIQLPP LYTN
Sbjct: 1507 ILQLKESIPSFLQK--DLKDRFETPFDFVIPRRNVRPQLINQLSPKSYTSIQLPPNLYTN 1566
Query: 1568 NDWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWL 1627
DW+GF V TL INK+PTAI NN+GS H+L+CQF IE GL+ PLHIHT +E+ +WL
Sbjct: 1567 TDWMGFAVWTLFQINKHPTAILNNVGSVSRHELICQFGIENGLINPLHIHTIIEDTVIWL 1626
Query: 1628 DERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVHNQDREKIDQILI 1640
ERQFVWLYY+PR+ +G I R S+V A +EADSP+L + CG+ LV+ Q+ + ID+IL+
Sbjct: 1627 HERQFVWLYYSPRKKYGQIFRHRSHVWAIIEADSPDLMAKCCGLQLVYKQNVQMIDKILM 1668
BLAST of Lsi08G001180 vs. NCBI nr
Match:
XP_038889439.1 (TMV resistance protein N-like [Benincasa hispida])
HSP 1 Score: 2085.1 bits (5401), Expect = 0.0e+00
Identity = 1059/1685 (62.85%), Postives = 1289/1685 (76.50%), Query Frame = 0
Query: 7 SMERGASITSLSSPPSPPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVV 66
+MER ASITSLSSPP S+SL LPPLQNYDVFLSHRAKDTGRSFTADLH+AL +GIVV
Sbjct: 6 TMERRASITSLSSPPPRYSISLPLPPLQNYDVFLSHRAKDTGRSFTADLHDALTDKGIVV 65
Query: 67 FRDDVDEDDGGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRDQLVLP 126
FRDDVDE+DGGKPL EKMKAVEESRSSIVVFSENYGNL CMKE+ KIVMCKELRDQLVLP
Sbjct: 66 FRDDVDEEDGGKPLTEKMKAVEESRSSIVVFSENYGNLVCMKEIEKIVMCKELRDQLVLP 125
Query: 127 IFYQIDPANVRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQDSQ--- 186
IFY I+PA+VR QKGNFEKHF EHEAN I+ EEV++W+YSM Q+GHLSGWHLQDSQ
Sbjct: 126 IFYLINPAHVRNQKGNFEKHFIEHEANPEINIEEVKSWKYSMQQVGHLSGWHLQDSQSEA 185
Query: 187 -------------------------------LHQVNRLLGIGLDDIRFVGIWGMGGIGKT 246
LHQ+N L+GIGLDD+RFVGIWGMGGIGKT
Sbjct: 186 GTINEVVMHIFNKLRPDLFRYDDKLVGISSRLHQINMLMGIGLDDVRFVGIWGMGGIGKT 245
Query: 247 TLARIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGATLI 306
T+ARIIYKSVSHLFE YFLDNVKEALK EGLASLQEKLLTGALMKRNI+IPNADGATLI
Sbjct: 246 TIARIIYKSVSHLFERYYFLDNVKEALKKEGLASLQEKLLTGALMKRNIDIPNADGATLI 305
Query: 307 KRRISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVE 366
KRRIS++K LI+LDD++HLSQLQ+LAGG DWFG GSRVI+TTR+EHLL+SHGIERRY VE
Sbjct: 306 KRRISNLKVLIILDDIDHLSQLQKLAGGLDWFGSGSRVIVTTRNEHLLISHGIERRYNVE 365
Query: 367 GLNVEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWEN 426
GL +EEALQLFSQKAFG +HPKKGY+DLS QVV Y G LPLAIEVLGSSLR+K M W N
Sbjct: 366 GLKIEEALQLFSQKAFGEDHPKKGYYDLSSQVVSYAGGLPLAIEVLGSSLRNKPMKDWTN 425
Query: 427 AVEKLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAII 486
AVEKL EV DK+I E LKISY +LEE EQ+IFLD+ACFFKKKSK+QAIE+LQSF F A++
Sbjct: 426 AVEKLWEVRDKEILEKLKISYYMLEESEQKIFLDIACFFKKKSKKQAIEILQSFEFLAVL 485
Query: 487 GLEILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQ 546
GLEILEEKSLITTPH+KIQMHDLIQEMGQ IV FPN PEKRSRLWLR+D+N ALS D+
Sbjct: 486 GLEILEEKSLITTPHDKIQMHDLIQEMGQRIVSENFPNEPEKRSRLWLREDINRALSRDK 545
Query: 547 GTEAIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLNWHGY 606
GTEAI GI+MD+ EEGES LNAK+FSAMTNL++LK+NNVYLS +L+YLSDQLRFLNWHGY
Sbjct: 546 GTEAIGGIMMDMDEEGESHLNAKSFSAMTNLRVLKVNNVYLSEELQYLSDQLRFLNWHGY 605
Query: 607 PLE-VHVNYQLSYVF-------LVISYYCLKYRFDKLKAINLSDSQFISKTPDFFGVPNL 666
PL+ + N+ + + + + + LK INLSDSQF+SKTPDF GVPNL
Sbjct: 606 PLKCLPSNFNPTNLLELELPNSSIQHLWTTSKSLETLKVINLSDSQFLSKTPDFSGVPNL 665
Query: 667 ERLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKF 726
ERL+LSGC +++LH SLG LKHLI LDL+NCK L IPFNI LESL IL LSGCS L
Sbjct: 666 ERLVLSGCVDIHQLHHSLGNLKHLIQLDLRNCKKLTTIPFNICLESLHILVLSGCSNLTH 725
Query: 727 FPKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKT 786
FPKI GNM +L+E HLD TSI+ LH SIG LT LVLLNL+NCTNLL+LP+TIG L SLKT
Sbjct: 726 FPKISGNMNHLLELHLDETSIKNLHSSIGHLTALVLLNLKNCTNLLKLPSTIGCLTSLKT 785
Query: 787 LTLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHS 846
L L+GCSKLD +PESLG I CLEKLD+T TC+NQAP+SLQLLT LEIL+CQGLSRKF+ S
Sbjct: 786 LNLNGCSKLDSLPESLGNIFCLEKLDITNTCVNQAPMSLQLLTKLEILNCQGLSRKFLQS 845
Query: 847 LFPSWNYSSHYSS-QGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLS 906
LFP+WN++ +S QGLK+T F CS+R LNLSDC L DGD+PN+L+SL S+QIL LS
Sbjct: 846 LFPTWNFTRKFSHYQGLKVTNWFHFGCSLRILNLSDCNLWDGDLPNDLRSLASLQILHLS 905
Query: 907 GNNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQEKQMP 966
N+FT LP+S+ LV+LR L LV C L LPKLPLSVR+VEARDCVSL EYYNQEKQ+P
Sbjct: 906 QNHFTKLPESISHLVNLRDLFLVECSHLLSLPKLPLSVRDVEARDCVSLNEYYNQEKQIP 965
Query: 967 SSSTGMAVISCPITNE-EKNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIPYTQL 1026
SS GM I CPI+NE +++KID+ LS+IHLRTM QRYIEVLTWQQE+YFF IPY
Sbjct: 966 SSEMGMTFIRCPISNEPSESYKIDQPRLSAIHLRTMAQRYIEVLTWQQEKYFFVIPYPSF 1025
Query: 1027 ISCFDQRKLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNP-QSVGLSETFCNFII 1086
I+CFD+++ G SIT HC D I+E+N RIGIAL A F+V ++ + S+ C FI+
Sbjct: 1026 IACFDEKRYGFSITAHCPPD--YINEENPRIGIALGASFEVQKHEISNNNNSKICCEFIV 1085
Query: 1087 KLETDNCPLKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTNPMVK 1146
K+ETD CPLKS ++F+ N+D+L P GL VFYIP R IS WLNQ CC+D+SIM NP+VK
Sbjct: 1086 KMETDECPLKSALVFDGNKDELESPVGLSVFYIPMRRISGWLNQCCCIDVSIMTDNPLVK 1145
Query: 1147 VKACGVSLLFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGGANAR 1206
VK CG S+L+++N G FIGKI+K FGSP H +VDHILNRQN VDVS+L++GGA+ +
Sbjct: 1146 VKWCGASILYEQNAGSFIGKIIKAFFGSPGRYHTSIVDHILNRQNRVDVSTLLDGGAHYK 1205
Query: 1207 SYWLNALHRTVGTLPKLRPSVQSND-IEGCSSSNVAIEQVLSENDSTIMLKRNLKS---- 1266
+ W NAL RT+G+ P+LRPS + IE CS+ N + E +E+D +IMLKRN+K+
Sbjct: 1206 TTWFNALQRTIGSFPRLRPSRPPREVIEDCSTMNASSEIDENESDYSIMLKRNIKATLER 1265
Query: 1267 ---ELKLNGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAIFGSDN 1326
ELKL GEYYIFP+ EIS+ WFN Q+K+ +TI++ P LHK+KKWMGL FF +F +D
Sbjct: 1266 TFEELKLYGEYYIFPQKEISRSWFNFQLKEPKITIKISPNLHKDKKWMGLAFFVVFSADE 1325
Query: 1327 KSNISQSFKYQLECDKYHLGRPSVIRLHDGAFNDSHQLWVSYEPRAVYPYSLNNWRHVRV 1386
S S SF YQ+E D+Y + R S+I L++ F+DSHQLW+ +EPRAVYPY LN WRH+R
Sbjct: 1326 NSPKSHSFSYQVENDEYTMQRQSIIYLNEELFDDSHQLWMFFEPRAVYPYRLNQWRHLRF 1385
Query: 1387 SFLPNCTQTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGMMSLIRS 1446
+F+ N + K +LCGARL+YKQD+ FV I+ +VL + LHEFY+ + KGM+ ++
Sbjct: 1386 AFVCNDSDFKAVLCGARLVYKQDVEGFVNTIVSNVLSSPVELHEFYDQSYVKGMLRNVQF 1445
Query: 1447 QKYDP-NIEDEDDEEEIETRGENYASTSSSSLVPTTKSLDDSNDYYFELKKCLHCSGELT 1506
KYDP N E+E ++ + E ++++ +T S + + +LK+ +
Sbjct: 1446 HKYDPKNKEEETRQDLLIQEWEEEQNSNAYPQQDSTSSPNMERSHILQLKESI------- 1505
Query: 1507 RTSTNLTGQPTRSYNRQVVDLPNRYDTAFDFIIRGHDVPQLFSRQPERNRASIQLPPTLY 1566
S+ ++ D +R+ FDF+I + PQLF++ +N I+LPP LY
Sbjct: 1506 -----------PSFLQK--DSKDRFQNTFDFVIPRRNFPQLFNQLSPKNHTGIELPPNLY 1565
Query: 1567 TNNDWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLHIHTTMENKWL 1626
T NDW+GF+VCTL +NK+PTAI NNLGS H+L+CQF IE GL++PLH+H+ E++++
Sbjct: 1566 TTNDWMGFLVCTLFQVNKHPTAILNNLGSITRHELICQFAIENGLIEPLHLHSITEDRFI 1625
Query: 1627 WLDERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVHNQDREKIDQI 1638
WL ERQFVWLYY+PR T+G I R S + A +EAD+P+L VR CG+ LV+ QD E ID+I
Sbjct: 1626 WLHERQFVWLYYSPRNTYGEIFRHRSCIWAIIEADTPDLMVRCCGLQLVYKQDMEVIDKI 1668
BLAST of Lsi08G001180 vs. NCBI nr
Match:
XP_008463577.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo])
HSP 1 Score: 2055.0 bits (5323), Expect = 0.0e+00
Identity = 1046/1684 (62.11%), Postives = 1272/1684 (75.53%), Query Frame = 0
Query: 10 RGASITSLSSPPSPPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRD 69
R +SI SLSSPP P SLS LPPL+NYDVFLSHRAKDTG SF ADLH+AL SQGIVV+RD
Sbjct: 10 RVSSIASLSSPPPPYSLSFPLPPLRNYDVFLSHRAKDTGCSFAADLHKALTSQGIVVYRD 69
Query: 70 DVDEDDGGKPLAEKMKAVEESRSSIVVFSENYGNLACMKEVGKIVMCKELRDQLVLPIFY 129
+E+ GKPL EKMKAVEESR SIV+FSENYGNL CMKE+ KIVMCKEL DQLVLPIFY
Sbjct: 70 HENEEGRGKPLVEKMKAVEESRCSIVIFSENYGNLVCMKEIEKIVMCKELMDQLVLPIFY 129
Query: 130 QIDPANVRKQKGNFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQDSQ------ 189
+IDP NVRKQKGNFEKHFNEHEAN ID EEV++WRYSM Q+GHLSGWH+QDSQ
Sbjct: 130 KIDPTNVRKQKGNFEKHFNEHEANHEIDIEEVESWRYSMKQVGHLSGWHIQDSQSEAGAI 189
Query: 190 ----------------------------LHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLA 249
LHQ+N LLGIGLDD+RFVGIWGMGGIGKTTLA
Sbjct: 190 DEIVKHIFNKLRPDLFRYDDKLVGISPRLHQINMLLGIGLDDVRFVGIWGMGGIGKTTLA 249
Query: 250 RIIYKSVSHLFEGCYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGATLIKRR 309
RIIY+SVSHLFEGCYFLDNVKEALK EGLASLQEKLLTGALMKRNI+IPNADGATLIKRR
Sbjct: 250 RIIYRSVSHLFEGCYFLDNVKEALKKEGLASLQEKLLTGALMKRNIDIPNADGATLIKRR 309
Query: 310 ISSIKALIVLDDVNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLN 369
IS++KALI+LDDVNHLSQLQ+LAGG DWFGPGSRVI+TTRDEHLL+SHGIERRY VEGL
Sbjct: 310 ISNLKALIILDDVNHLSQLQKLAGGSDWFGPGSRVIVTTRDEHLLISHGIERRYNVEGLK 369
Query: 370 VEEALQLFSQKAFGTNHPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVE 429
+EEALQLFSQKAFG +HP+KGYFD+ QVV+Y G LPLAIEV GSSLR+K M+ WENAVE
Sbjct: 370 IEEALQLFSQKAFGKDHPEKGYFDVCSQVVDYCGGLPLAIEVFGSSLRNKPMEQWENAVE 429
Query: 430 KLKEVGDKKIFEILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLE 489
KLKEV DKKI E LKI Y +LE+ EQ+IFLD+ACFFK+KSK+QAIE+L+SFGF A++GLE
Sbjct: 430 KLKEVCDKKILEKLKIGYYMLEKSEQKIFLDIACFFKRKSKRQAIEILESFGFPAVLGLE 489
Query: 490 ILEEKSLITTPHEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTE 549
ILEEKSLIT PH+KIQMHDLIQEMGQEIVR+ FPN PEKRSRLWLR+D+NLALS D+GTE
Sbjct: 490 ILEEKSLITVPHDKIQMHDLIQEMGQEIVRQNFPNEPEKRSRLWLREDINLALSRDEGTE 549
Query: 550 AIKGIVMDLSEEGESQLNAKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLNWHGYPL- 609
AI+GI+MDL EEGES LNAK+FSAMTNL++LK+NNV+L ++EYLSDQLRF+NWHGYPL
Sbjct: 550 AIEGIMMDLDEEGESHLNAKSFSAMTNLRVLKVNNVHLCEEIEYLSDQLRFINWHGYPLT 609
Query: 610 EVHVNYQLSYVF-------LVISYYCLKYRFDKLKAINLSDSQFISKTPDFFGVPNLERL 669
+ N+ + + + + + + LK INLSDSQF+SKTPD GVP LERL
Sbjct: 610 TLPSNFNPTNLLELELPNSSIQNLWTASKSLETLKVINLSDSQFLSKTPDLSGVPYLERL 669
Query: 670 ILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKFFPK 729
+LSGC L++LH SLG LKHL LDLK+CK L IPFNI LESL LSGCS L FPK
Sbjct: 670 VLSGCVELHQLHHSLGNLKHLTQLDLKHCKKLTSIPFNICLESLNTFVLSGCSNLTHFPK 729
Query: 730 IVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTLTL 789
I NM +L+E HLD TSI+ LH SIG LTGLVLLNL NCTNLL+LP TIG L SLK+L L
Sbjct: 730 ISANMNHLLELHLDETSIKTLHSSIGHLTGLVLLNLRNCTNLLKLPTTIGCLTSLKSLNL 789
Query: 790 HGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFP 849
HGCSKLD +PESLG I+CLEKLD+T TC+NQAP+SLQLLT LEIL+CQGLSRKF+HSLFP
Sbjct: 790 HGCSKLDSLPESLGNISCLEKLDITSTCVNQAPMSLQLLTKLEILNCQGLSRKFLHSLFP 849
Query: 850 SWNYSSHYS-SQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSGNN 909
+WN++ +S SQGLK+T F+ CS+R LNLSDC L DGD+PN+L SL S+Q+LDLS N+
Sbjct: 850 TWNFTRKFSNSQGLKVTIWFNFGCSLRVLNLSDCNLWDGDLPNDLHSLASLQVLDLSQNH 909
Query: 910 FTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEYYNQEKQMPSSS 969
FT LP+S+ LV+LR L LV C L LPKLPLSVR+V+ARDCVSLKEYYNQEKQ+PSS
Sbjct: 910 FTKLPESIRHLVNLRGLFLVECFHLLCLPKLPLSVRDVDARDCVSLKEYYNQEKQIPSSE 969
Query: 970 TGMAVISCPITNE-EKNFKIDRVNLSSIHLRTMVQRYIEVLTWQQEEYFFTIPYTQLISC 1029
GM +I CPITNE +++KI + LS+IHLRT QRY+EVLTWQQE+YFF IPY I+C
Sbjct: 970 MGMTIIRCPITNEPTQSYKIHQPALSAIHLRTTTQRYLEVLTWQQEQYFFVIPYPNFIAC 1029
Query: 1030 FDQRKLGSSITVHCHEDTCEISEDNERIGIALSAFFQVLQNPQSVGLS-ETFCNFIIKLE 1089
FD+++ G SIT HC D +SEDN RIGIAL A F+V ++ S S + C+FI+K+E
Sbjct: 1030 FDEKRYGFSITAHCPPD--YVSEDNPRIGIALGAAFEVQKHEISNNNSPKVCCDFIVKME 1089
Query: 1090 TDNCPLKSPIIFNRNEDKLHPPQGLLVFYIPFRMISYWLNQSCCVDISIMPTNPMVKVKA 1149
TD CPLKSP++F+ N+D+L GL VFYIP IS WLNQ CC+++SI+ NP VKVK
Sbjct: 1090 TDECPLKSPLVFDGNKDELKSQMGLSVFYIPTNRISRWLNQCCCIEVSIITDNPFVKVKW 1149
Query: 1150 CGVSLLFQKNGGMFIGKIMKGLFGSPDFVHKFMVDHILNRQNHVDVSSLVEGGANARSYW 1209
CG S+L+++N G FIGKI+K LFGSPD H +VDH+LNRQN VDVS+L++GGA ++ W
Sbjct: 1150 CGASILYEQNAGSFIGKIIKALFGSPDKYHTSIVDHLLNRQNRVDVSTLLDGGARYKTSW 1209
Query: 1210 LNALHRTVGTLPKLRPSVQSND-IEGCSSSNVAIEQVLSENDSTIMLKRNLKS------- 1269
NAL RT+G+ P+LRPS Q + + CS+ N E SE+D +IMLKRNL +
Sbjct: 1210 FNALQRTIGSFPRLRPSKQPREAMLDCSTMNATFEGEESESDYSIMLKRNLTATLLRTFE 1269
Query: 1270 ELKLNGEYYIFPRGEISKRWFNLQVKKASVTIEVPPKLHKNKKWMGLTFFAIFGSDNKSN 1329
ELKL EYYIFP+ E+S+R+FN Q+++ +TI++PP LHK+KKWMGL FF +F D S
Sbjct: 1270 ELKLYAEYYIFPQKEMSRRFFNFQLEEPKITIKIPPNLHKDKKWMGLAFFVVFSVDENSQ 1329
Query: 1330 ISQSFKYQLECDKYHLGRPSVIRLHDGAFNDSHQLWVSYEPRAVYPYSLNNWRHVRVSFL 1389
S SF YQ++ D+Y + R S++ L+ SHQLWV +EPRAVYPY LN WRH+R S +
Sbjct: 1330 KSHSFSYQVDNDEYRMERESMLYLNKDLLVGSHQLWVFFEPRAVYPYRLNQWRHLRFSIV 1389
Query: 1390 PNCTQTKVILCGARLLYKQDLNEFVQAIIDSVLGCSLNLHEFYNGVFSKGMMSLIRSQKY 1449
N + K +LCGA L+YKQDL FV I+ +VL LHEFY+ + + ++ + KY
Sbjct: 1390 CNNSDFKAVLCGANLVYKQDLEGFVNIIVSNVLSSPAELHEFYDRSYVESILRNVHCHKY 1449
Query: 1450 DPNIEDEDDEEEIETRGENYASTSSSSLVPTTKSLDDSNDYYFELKKCLHCSGELTRTST 1509
DP + D + R E + S+ P + +DS+ S + R+
Sbjct: 1450 DPKKNENDQRRQDHLRIEKWVEEQDSNAHP--QEDEDSSS-----------SSNMERSHF 1509
Query: 1510 NLTGQPTRSYNRQVVDLPNRYDTAFDFIIRGHDV-PQLFSRQPERNRASIQLPPTLYTNN 1569
+L Q S+ ++ DL +RY+ FDF+I ++ PQL ++ RN IQLPP YTN
Sbjct: 1510 SLLKQSIPSFLQK--DLKDRYEMTFDFVIPRRNIRPQLINQLSPRNYTRIQLPPNSYTNI 1569
Query: 1570 DWIGFIVCTLLYINKNPTAIFNNLGSRMPHDLMCQFEIEQGLLKPLHIHTTMENKWLWLD 1629
DW+GF V T+ INK+PTAI NNLGS H+L+CQF IE GL+ PLHIH+ +E+K +WL
Sbjct: 1570 DWMGFAVWTVFQINKHPTAILNNLGSVSRHELICQFGIENGLINPLHIHSIIEDKVIWLH 1629
Query: 1630 ERQFVWLYYTPRRTFGNILRQCSYVRANVEADSPELAVRRCGIYLVHNQDREKIDQILIE 1640
ERQFVWLYY+PR+ +G I R S+V A +EAD+P+L V CG+ +V+ +D ID+IL+E
Sbjct: 1630 ERQFVWLYYSPRKKYGEIFRHRSHVWAIIEADTPDLVVICCGLQVVYKKDVRVIDKILME 1676
BLAST of Lsi08G001180 vs. TAIR 10
Match:
AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )
HSP 1 Score: 499.2 bits (1284), Expect = 1.3e-140
Identity = 366/1134 (32.28%), Postives = 564/1134 (49.74%), Query Frame = 0
Query: 23 PPSLSLALPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAE 82
P S S + + DVF+S R +D ++F + L GI FRDD+D G E
Sbjct: 5 PSSSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPE 64
Query: 83 KMKAVEESRSSIVVFSENYGNLA-CMKEVGKIVMCKELRDQLVLPIFYQIDPANVRKQKG 142
+ A++ SR +IVV S NY + C+ E+ KI+ C + ++PIFY++DP++VR+Q+G
Sbjct: 65 LIDAIKGSRFAIVVVSRNYAASSWCLDELLKIMECNK---DTIVPIFYEVDPSDVRRQRG 124
Query: 143 NFEKHFNEHEANLGIDNEEVQNWRYSMNQLGHLSG-----W-----------HLQD---- 202
+F + H D E+V W+ ++ +L +SG W + D
Sbjct: 125 SFGEDVESHS-----DKEKVGKWKEALKKLAAISGEDSRNWDDSKLIKKIVKDISDKLVS 184
Query: 203 -------------SQLHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEG 262
S + + ++ I D+R +GIWGMGG+GKTT+A+ +Y +S F+
Sbjct: 185 TSWDDSKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQV 244
Query: 263 CYFLDNVKEALKNEGLASLQEKLLTGALMKRNIEI-PNADGATLIKRRISSIKALIVLDD 322
F++NVKE G+ LQ + L +R+ E + +IK R IVLDD
Sbjct: 245 HCFMENVKEVCNRYGVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVLDD 304
Query: 323 VNHLSQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKA 382
V+ QL EL WFGPGSR+I+TTRD HLL+SHGI YKV+ L +EALQLF A
Sbjct: 305 VDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLVYKVKCLPKKEALQLFCNYA 364
Query: 383 FGTN-HPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIF 442
F G+ +LS+Q V Y LPLA+ VLGS L +S WE+ + +LK I
Sbjct: 365 FREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLKTYPHSDIM 424
Query: 443 EILKISYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTP 502
E+L++SYD L+E E+ IFL ++CF+ K ++L G+AA IG+ IL EKSLI
Sbjct: 425 EVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILTEKSLIVES 484
Query: 503 HEKIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVMDLSE 562
+ +++HDL+++MG+E+VR+ NNP +R LW +D+ LS + GT+ ++GI ++LSE
Sbjct: 485 NGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEGISLNLSE 544
Query: 563 EGESQLNAKAFSAMTNLKMLKI--------NNVYLSGDLEYLSDQLRFLNWHGYPLEVHV 622
E + +AF ++NLK+L V+L L YL +LR+L W GYPL+
Sbjct: 545 ISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMP 604
Query: 623 N--YQLSYVFLVISYYCLKYRFD------KLKAINLSDSQFISKTPDFFGVPNLERLILS 682
+ + V L +S L+ +D LK ++LS +++ + PD NLE L LS
Sbjct: 605 SRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKATNLEELNLS 664
Query: 683 GCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSGCSRLKFFPKIVG 742
C L ++ S+ LK L L NC LKDIP I L+SL + +SGCS LK FP+I
Sbjct: 665 YCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVGMSGCSSLKHFPEISW 724
Query: 743 NMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGC 802
N + L +L T I+EL SI +L+ LV L++ +C L LP+ +G L+SLK+L L GC
Sbjct: 725 NTRRL---YLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKSLNLDGC 784
Query: 803 SKLDRIPESLGTIACLEKLDVTGTCIN--------------------------------- 862
+L+ +P++L + LE L+V+G C+N
Sbjct: 785 RRLENLPDTLQNLTSLETLEVSG-CLNVNEFPRVSTSIEVLRISETSIEEIPARICNLSQ 844
Query: 863 -------------QAPLSLQLLTNLEILDCQG----------------------LSRKFI 922
P+S+ L +LE L G L R I
Sbjct: 845 LRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLEICQTMSCLRWFDLDRTSI 904
Query: 923 HSLFPS---------------------WNY------------SSHYSSQGLKLTYC--FS 982
L + W+ +S ++ +GL + C S
Sbjct: 905 KELPENIGNLVALEVLQASRTVIRRAPWSIARLTRLQVLAIGNSFFTPEGLLHSLCPPLS 964
Query: 983 SFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSGNNFTCLPKSLGQLVSLRTLLLVN 998
F +R L+LS+ + +IPN++ +L ++ LDLSGNNF +P S+ +L L L L N
Sbjct: 965 RFDDLRALSLSNMNMT--EIPNSIGNLWNLLELDLSGNNFEFIPASIKRLTRLNRLNLNN 1024
BLAST of Lsi08G001180 vs. TAIR 10
Match:
AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 464.5 bits (1194), Expect = 3.4e-130
Identity = 353/1069 (33.02%), Postives = 542/1069 (50.70%), Query Frame = 0
Query: 31 PPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAEKMKAVEES 90
P +DVFLS R DT +FT L +AL +GI F D D G L +E+S
Sbjct: 6 PSSAEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID--DRLRRGDNLTALFDRIEKS 65
Query: 91 RSSIVVFSENYGNLA-CMKEVGKIVMCKELRDQLVLPIFYQIDPANVRKQKGNFEKHFNE 150
+ +I+VFS NY N A C++E+ KI+ C+ QLV+PIFY++D ++V KQ+ +F F
Sbjct: 66 KIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPFKL 125
Query: 151 HEANL-GIDNEEVQNWRYSMNQLGHLSGWHLQ---------------------------- 210
E G+ EE+ +W+ ++ ++ G+ ++
Sbjct: 126 PELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLNDLAPSG 185
Query: 211 -------DSQLHQVNRLLG-IGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFL 270
+S+L + +LL LD + +GI GM GIGKTTLA +Y + F+G FL
Sbjct: 186 NEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGSCFL 245
Query: 271 DNVKEALKNEGLASLQEKLLTGALMKRNIEIPNADGA-TLIKRRISSIKALIVLDDVNHL 330
N++E GL SL +KL + L R++EI A +RR+ S + LIVLDDVN
Sbjct: 246 TNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDVNDE 305
Query: 331 SQLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTN 390
Q++ L G W+ GSR+IITTRD L+ + R+Y + LN EAL+LFS AF +
Sbjct: 306 KQIRYLMGHCKWYQGGSRIIITTRDSKLIETIK-GRKYVLPKLNDREALKLFSLNAFSNS 365
Query: 391 HPKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKI 450
P K + L+ V++Y PLA++VLGS L ++ WE +++LK I+E+L+
Sbjct: 366 FPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSHGDIYEVLET 425
Query: 451 SYDLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTPHEKIQ 510
SY+ L ++ +FLD+ACFF+ ++ +L S G ++ L +K LIT +I+
Sbjct: 426 SYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITLSDNRIE 485
Query: 511 MHDLIQEMGQEIVRRM-------------FPNNPEKRSRLWLRKDVNLALSHDQGTEAIK 570
MHD++Q M +EI ++ N + RLW +D+ L+ GT+ I+
Sbjct: 486 MHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEGLGTDKIR 545
Query: 571 GIVMDLSEEGESQLNAKAFSAMTNLKMLKINN------------VYLSGDLEYLSDQLRF 630
GI +D S+ +L+AKAF M NLK LKI + ++L L +L ++L +
Sbjct: 546 GIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSFLPNELTY 605
Query: 631 LNWHGYPLE-VHVNYQ-LSYVFLVISYYCLKYRFD------KLKAINLSDSQFISKTPDF 690
L+WHGYPL+ + +++ + V L + + L+ +D LK ++LS S + +
Sbjct: 606 LHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSINLRQCLGL 665
Query: 691 FGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLKNCKCLKDIPFNISLESLVILTLSG 750
NLERL L GC L KL ++ L+ LI L+L++C L+ +P I +SL L LSG
Sbjct: 666 ANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQSLQTLILSG 725
Query: 751 CSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGS 810
CS LK FP I N++ L+ LDGT I+ L SI L LLNL+NC L L + +
Sbjct: 726 CSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFRRLALLNLKNCKKLKHLSSDLYK 785
Query: 811 LISLKTLTLHGCSKLDRIPESLGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLS 870
L L+ L L GCS+L+ PE + LE L + T I + P + L+N++ G S
Sbjct: 786 LKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMP-KMMHLSNIKTFSLCGTS 845
Query: 871 RKFIHSLF---PSWNYSSHYSSQGLKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLP 930
S+F P+ S +LT + S CS+ KL P+N+ L
Sbjct: 846 SHVSVSMFFMPPTLGCS--------RLTDLYLSRCSLYKL------------PDNIGGLS 905
Query: 931 SVQILDLSGNNFTCLPKSLGQLVSLRTLLLVNCKRLQELPKLPLSVRNVEARDCVSLKEY 990
S+Q L LSGNN LP+S QL +L+ L CK L+ LP LP +++ ++A +C SL+
Sbjct: 906 SLQSLCLSGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETL 965
Query: 991 YNQEKQMPSSST--GMAVIS-CPITNEEKNFKI---DRVNLSSIHLRTMVQRYIEVLTWQ 1019
N + M + S C N++ + R+ S + +RY
Sbjct: 966 ANPLTPLTVGERIHSMFIFSNCYKLNQDAQASLVGHARIK-SQLMANASAKRYYRGFV-P 1025
BLAST of Lsi08G001180 vs. TAIR 10
Match:
AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 445.7 bits (1145), Expect = 1.7e-124
Identity = 316/934 (33.83%), Postives = 479/934 (51.28%), Query Frame = 0
Query: 30 LPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAEKMKAVEE 89
+P YDVF+S R D ++F + L+++L GI F DDV+ G E + A+E
Sbjct: 8 IPERWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIET 67
Query: 90 SRSSIVVFSENYGNLA-CMKEVGKIVMC-KELRDQLVLPIFYQIDPANVRKQKGNFEKHF 149
S+ IVV +++Y + A C+ E+ I+ K +V PIF +DP+++R Q+G++ K F
Sbjct: 68 SKILIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSF 127
Query: 150 NEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQD-------------------------- 209
++H+ + ++ ++++WR ++ ++ ++SGW +++
Sbjct: 128 SKHKNSHPLN--KLKDWREALTKVANISGWDIKNRNEAECIADITREILKRLPCQYLHVP 187
Query: 210 -------SQLHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFLDN 269
S+L ++ LL IG D +R + I+GMGGIGKTTLA++ + SHLFEG FL+N
Sbjct: 188 SYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLEN 247
Query: 270 VKE-ALKNEGLASLQEKLLTGALMKRNIEIPNADGATLIKRRISSIKALIVLDDVNHLSQ 329
+E + K EG LQ +LL+ L + +IE D A +K R S + L+V+DDV+ + Q
Sbjct: 248 FREYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA--VKERFRSKRVLLVVDDVDDVHQ 307
Query: 330 LQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTNHP 389
L A D FG GSR+IITTR+ HLL E Y + L+ +E+L+LFS AF T+ P
Sbjct: 308 LNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEP 367
Query: 390 KKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKISY 449
K + S +VV Y LPLA+EVLG+ L ++S+ WE+ ++ LK + + I L+IS+
Sbjct: 368 PKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISF 427
Query: 450 DLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTPHEKIQMH 509
+ L ++++FLD+ACFF +L I L +L E+ LIT I MH
Sbjct: 428 NALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMH 487
Query: 510 DLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVMDLSEEGESQLN 569
DL+++MG++IVR + P +RSRLW DV L GT AI+G+ +
Sbjct: 488 DLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFE 547
Query: 570 AKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLNWHGYPLEVH-VNYQL-SYVFLVISY 629
+AF+ M L++L++ V L+G E+ LR+L WHG+ LE +N L S L + Y
Sbjct: 548 VEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQY 607
Query: 630 YCLKYRFDK----------LKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLHQS 689
LK RF K +K ++LS S ++ +TPDF PN+E+LIL C L +H+S
Sbjct: 608 SNLK-RFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKS 667
Query: 690 LGTL-KHLILLDLKNCKCLKDIPFNI-SLESLVILTLSGCSRLKFFPKIVGNMKNLIEFH 749
+G L K L+LL+L +C L +P I L+SL L LS CS+L
Sbjct: 668 IGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKL----------------- 727
Query: 750 LDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGCSKLDRIPES 809
+ L ++G+L L L L + T L E+P+TI L LK L+L+G
Sbjct: 728 ------ERLDDALGELESLTTL-LADFTALREIPSTINQLKKLKRLSLNG---------- 787
Query: 810 LGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPSWNYSSHYSSQG 869
C+GL I +L YS S
Sbjct: 788 ----------------------------------CKGLLSDDIDNL-----YSEKSHSVS 847
Query: 870 LKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSGNNFTCLPKSLGQLVS 914
L S MR L+L C L D IP ++ SL ++ LDL GN+F LP L +
Sbjct: 848 LLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNLPTDFATLPN 863
BLAST of Lsi08G001180 vs. TAIR 10
Match:
AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 445.7 bits (1145), Expect = 1.7e-124
Identity = 316/934 (33.83%), Postives = 479/934 (51.28%), Query Frame = 0
Query: 30 LPPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAEKMKAVEE 89
+P YDVF+S R D ++F + L+++L GI F DDV+ G E + A+E
Sbjct: 11 IPERWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIET 70
Query: 90 SRSSIVVFSENYGNLA-CMKEVGKIVMC-KELRDQLVLPIFYQIDPANVRKQKGNFEKHF 149
S+ IVV +++Y + A C+ E+ I+ K +V PIF +DP+++R Q+G++ K F
Sbjct: 71 SKILIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSF 130
Query: 150 NEHEANLGIDNEEVQNWRYSMNQLGHLSGWHLQD-------------------------- 209
++H+ + ++ ++++WR ++ ++ ++SGW +++
Sbjct: 131 SKHKNSHPLN--KLKDWREALTKVANISGWDIKNRNEAECIADITREILKRLPCQYLHVP 190
Query: 210 -------SQLHQVNRLLGIGLDDIRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFLDN 269
S+L ++ LL IG D +R + I+GMGGIGKTTLA++ + SHLFEG FL+N
Sbjct: 191 SYAVGLRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLEN 250
Query: 270 VKE-ALKNEGLASLQEKLLTGALMKRNIEIPNADGATLIKRRISSIKALIVLDDVNHLSQ 329
+E + K EG LQ +LL+ L + +IE D A +K R S + L+V+DDV+ + Q
Sbjct: 251 FREYSKKPEGRTHLQHQLLSDILRRNDIEFKGLDHA--VKERFRSKRVLLVVDDVDDVHQ 310
Query: 330 LQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTNHP 389
L A D FG GSR+IITTR+ HLL E Y + L+ +E+L+LFS AF T+ P
Sbjct: 311 LNSAAIDRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEP 370
Query: 390 KKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDVWENAVEKLKEVGDKKIFEILKISY 449
K + S +VV Y LPLA+EVLG+ L ++S+ WE+ ++ LK + + I L+IS+
Sbjct: 371 PKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISF 430
Query: 450 DLLEEPEQEIFLDLACFFKKKSKQQAIEVLQSFGFAAIIGLEILEEKSLITTPHEKIQMH 509
+ L ++++FLD+ACFF +L I L +L E+ LIT I MH
Sbjct: 431 NALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMH 490
Query: 510 DLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVMDLSEEGESQLN 569
DL+++MG++IVR + P +RSRLW DV L GT AI+G+ +
Sbjct: 491 DLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFE 550
Query: 570 AKAFSAMTNLKMLKINNVYLSGDLEYLSDQLRFLNWHGYPLEVH-VNYQL-SYVFLVISY 629
+AF+ M L++L++ V L+G E+ LR+L WHG+ LE +N L S L + Y
Sbjct: 551 VEAFAKMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQY 610
Query: 630 YCLKYRFDK----------LKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLHQS 689
LK RF K +K ++LS S ++ +TPDF PN+E+LIL C L +H+S
Sbjct: 611 SNLK-RFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKS 670
Query: 690 LGTL-KHLILLDLKNCKCLKDIPFNI-SLESLVILTLSGCSRLKFFPKIVGNMKNLIEFH 749
+G L K L+LL+L +C L +P I L+SL L LS CS+L
Sbjct: 671 IGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKL----------------- 730
Query: 750 LDGTSIQELHPSIGQLTGLVLLNLENCTNLLELPNTIGSLISLKTLTLHGCSKLDRIPES 809
+ L ++G+L L L L + T L E+P+TI L LK L+L+G
Sbjct: 731 ------ERLDDALGELESLTTL-LADFTALREIPSTINQLKKLKRLSLNG---------- 790
Query: 810 LGTIACLEKLDVTGTCINQAPLSLQLLTNLEILDCQGLSRKFIHSLFPSWNYSSHYSSQG 869
C+GL I +L YS S
Sbjct: 791 ----------------------------------CKGLLSDDIDNL-----YSEKSHSVS 850
Query: 870 LKLTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSGNNFTCLPKSLGQLVS 914
L S MR L+L C L D IP ++ SL ++ LDL GN+F LP L +
Sbjct: 851 LLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNLPTDFATLPN 866
BLAST of Lsi08G001180 vs. TAIR 10
Match:
AT1G27170.1 (transmembrane receptors;ATP binding )
HSP 1 Score: 433.0 bits (1112), Expect = 1.1e-120
Identity = 321/1059 (30.31%), Postives = 530/1059 (50.05%), Query Frame = 0
Query: 31 PPLQNYDVFLSHRAKDTGRSFTADLHEALASQGIVVFRDDVDEDDGGKPLAEKMKAVEES 90
P YDVFLS R DT +F L++AL + + VFRD+ + G + + +E+S
Sbjct: 9 PHRLKYDVFLSFRGADTRDNFGDHLYKALKDK-VRVFRDNEGMERGDEISSSLKAGMEDS 68
Query: 91 RSSIVVFSENY-GNLACMKEVGKIVMCKELRDQLVLPIFYQIDPANVRKQKGNFEKHFNE 150
+S++V S NY G+ C+ E+ + K D+ +LPIFY +DP++VRKQ + +K F E
Sbjct: 69 AASVIVISRNYSGSRWCLDELAMLCKMKSSLDRRILPIFYHVDPSHVRKQSDHIKKDFEE 128
Query: 151 HEANLGIDNEEVQNWRYSMNQLGHLSGW----HLQDSQLHQ--VNRLLG----------- 210
H+ + E+VQ WR ++ +G+L+G+ +D + + V R+L
Sbjct: 129 HQVRFSEEKEKVQEWREALTLVGNLAGYVCDKDSKDDDMIELVVKRVLAELSNTPEKVGE 188
Query: 211 --IGLDD----------------IRFVGIWGMGGIGKTTLARIIYKSVSHLFEGCYFLDN 270
+GL+ ++ +G++GMGGIGKTTLA+ Y + FE F+ +
Sbjct: 189 FIVGLESPLKDLTGLIDTESSSGVQVLGLYGMGGIGKTTLAKAFYNKIVGNFEQRAFISD 248
Query: 271 VKEALKNE-GLASLQEKLLTGALMKRNIEIPNAD-GATLIKRRISSIKALIVLDDVNHLS 330
++E E GL +LQ K L L + EI + G IK + K ++VLDDV+H+
Sbjct: 249 IRERSSAENGLVTLQ-KTLIKELFRLVPEIEDVSIGLEKIKANVHEKKIIVVLDDVDHID 308
Query: 331 QLQELAGGYDWFGPGSRVIITTRDEHLLVSHGIERRYKVEGLNVEEALQLFSQKAFGTNH 390
Q+ L G W+G G+ ++ITTRD +L + ++Y+V+ L +AL+LFS +
Sbjct: 309 QVHALVGETRWYGQGTLIVITTRDSEILSKLSVNQQYEVKCLTEPQALKLFSYHSLRKEE 368
Query: 391 PKKGYFDLSIQVVEYGGDLPLAIEVLGSSLRDKSMDV-WENAVEKLKEVGDKKIFEILKI 450
P K LS ++V+ G LPLA+EV GS L DK + W+ ++KLK+ + ++L++
Sbjct: 369 PTKNLLALSKKIVQISGLLPLAVEVFGSLLYDKKEEKDWQTQLDKLKKTQPGNLQDVLEL 428
Query: 451 SYDLLEEPEQEIFLDLACFFKKK--SKQQAIEVLQSFGFAAIIGLEILEEKSLI-TTPHE 510
S+ L++ E+++FLD+AC F K K + + VL+ G A L +L +KSL+ ++
Sbjct: 429 SFKSLDDEEKKVFLDIACLFLKMEIKKDEVVIVLKGCGLNAEAALSVLRQKSLVKILAND 488
Query: 511 KIQMHDLIQEMGQEIVRRMFPNNPEKRSRLWLRKDVNLALSHDQGTEAIKGIVMDLSEE- 570
+ MHD I++MG+++V + +P RSRLW R ++ L++ +GT +I+GIV+D ++
Sbjct: 489 TLWMHDQIRDMGRQMVLKESREDPGLRSRLWDRGEIMTVLNNMKGTSSIRGIVLDFKKKF 548
Query: 571 ------------------------------------------GESQLNAKAFSAMTNLKM 630
E + ++F+ MT L++
Sbjct: 549 ARDPTADEIVSRNLRNNPGIYSVFNYLKNKLVRFPAEEKPKSSEITIPVESFAPMTKLRL 608
Query: 631 LKINNVYLSGDLEYLSDQLRFLNWHGYPLE----VHVNYQLSYVFL----VISYYCLKYR 690
L+INNV L G+L+ L +L+++ W G PLE + QLS + L + L+ +
Sbjct: 609 LQINNVELEGNLKLLPSELKWIQWKGCPLENLPPDFLARQLSVLDLSESGIRQVQTLRNK 668
Query: 691 F--DKLKAINLSDSQFISKTPDFFGVPNLERLILSGCARLNKLHQSLGTLKHLILLDLKN 750
+ LK + L + PD LE+L+ C L K+ +S+G L+ LI LD +
Sbjct: 669 MVDENLKVVILRGCHSLEAIPDLSNHEALEKLVFEQCTLLVKVPKSVGNLRKLIHLDFRR 728
Query: 751 CKCLKDIPFNIS-LESLVILTLSGCSRLKFFPKIVGNMKNLIEFHLDGTSIQELHPSIGQ 810
C L + ++S L+ L L LSGCS L P+ +G M +L E LDGT+I+ L SI +
Sbjct: 729 CSKLSEFLVDVSGLKLLEKLFLSGCSDLSVLPENIGAMTSLKELLLDGTAIKNLPESINR 788
Query: 811 LTGLVLLNLENC----------------------TNLLELPNTIGSLISLKTLTLHGCSK 870
L L +L+L C T L LP++IG L +L+ L L C+
Sbjct: 789 LQNLEILSLRGCKIQELPLCIGTLKSLEKLYLDDTALKNLPSSIGDLKNLQDLHLVRCTS 848
Query: 871 LDRIPESLGTIACLEKLDVTGTCINQAPL---SLQLLTNLEILDCQGLSRKFIHSLFPSW 930
L +IP+S+ + L+KL + G+ + + PL SL L + DC+ L K + S
Sbjct: 849 LSKIPDSINELKSLKKLFINGSAVEELPLKPSSLPSLYDFSAGDCKFL--KQVPSSIGRL 908
Query: 931 N--YSSHYSSQGLK-LTYCFSSFCSMRKLNLSDCKLMDGDIPNNLQSLPSVQILDLSGNN 966
N SS ++ L + +R+L L +CK + +P ++ + ++ L+L G+N
Sbjct: 909 NSLLQLQLSSTPIEALPEEIGALHFIRELELRNCKFLK-FLPKSIGDMDTLYSLNLEGSN 968
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q40392 | 2.0e-154 | 38.73 | TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1 | [more] |
V9M398 | 2.5e-149 | 34.64 | Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... | [more] |
V9M2S5 | 2.7e-148 | 36.51 | Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... | [more] |
A0A290U7C4 | 4.5e-135 | 35.38 | Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... | [more] |
Q9SZ66 | 4.8e-129 | 33.02 | Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=... | [more] |
Match Name | E-value | Identity | Description | |
A0A5A7T7U3 | 0.0e+00 | 78.34 | TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... | [more] |
A0A1S3CAF1 | 0.0e+00 | 81.16 | LOW QUALITY PROTEIN: TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LO... | [more] |
A0A1S3CJJ5 | 0.0e+00 | 62.11 | TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103501686 PE=4 SV=1 | [more] |
A0A6J1EC12 | 0.0e+00 | 62.46 | TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... | [more] |
A0A6J1CK08 | 0.0e+00 | 61.82 | TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
Match Name | E-value | Identity | Description | |
KAA0039320.1 | 0.0e+00 | 78.34 | TMV resistance protein N-like [Cucumis melo var. makuwa] >TYK00503.1 TMV resista... | [more] |
XP_008459548.2 | 0.0e+00 | 81.16 | PREDICTED: LOW QUALITY PROTEIN: TMV resistance protein N-like [Cucumis melo] | [more] |
XP_038890618.1 | 0.0e+00 | 63.03 | TMV resistance protein N-like [Benincasa hispida] | [more] |
XP_038889439.1 | 0.0e+00 | 62.85 | TMV resistance protein N-like [Benincasa hispida] | [more] |
XP_008463577.1 | 0.0e+00 | 62.11 | PREDICTED: TMV resistance protein N-like [Cucumis melo] | [more] |
Match Name | E-value | Identity | Description | |
AT5G17680.1 | 1.3e-140 | 32.28 | disease resistance protein (TIR-NBS-LRR class), putative | [more] |
AT4G12010.1 | 3.4e-130 | 33.02 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT5G36930.1 | 1.7e-124 | 33.83 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT5G36930.2 | 1.7e-124 | 33.83 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT1G27170.1 | 1.1e-120 | 30.31 | transmembrane receptors;ATP binding | [more] |