Homology
BLAST of Lsi08G001160 vs. ExPASy Swiss-Prot
Match:
Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)
HSP 1 Score: 558.9 bits (1439), Expect = 1.5e-157
Identity = 380/1047 (36.29%), Postives = 579/1047 (55.30%), Query Frame = 0
Query: 11 SSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLA 70
+SSSS+S+W YDVFLSFRGEDTR FT HLY+ L +KGI TF+D+ +E G I +L
Sbjct: 2 ASSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCK 61
Query: 71 AIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFA 130
AIE S+FA+VV SENYA+SRWCL ELVKI EC+ R + TV+PIFY VDPSHVR Q SFA
Sbjct: 62 AIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFA 121
Query: 131 QAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRM-KPS 190
+AF +HE ++ +D + +++WR L + ANLK ++ I +I I ++ K S
Sbjct: 122 KAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKIS 181
Query: 191 LTVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRD- 250
L+ ++ +VGI+ L ++ SLL + V +GI GMGG+GKTTIAR ++ +
Sbjct: 182 LSYLQ--NIVGIDTHLEKIESLLEIGI---NGVRIMGIWGMGGVGKTTIARAIFDTLLGR 241
Query: 251 -----EFEAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFR 310
+F+ CFL D++EN G+ LQ LLS + K N + E+G + +
Sbjct: 242 MDSSYQFDGACFLKDIKEN--KRGMHSLQNALLSELLREKAN-YNNEEDGKHQMASRLRS 301
Query: 311 KKALLVLDDVERSDQIMG-LIPNKNSFGNGSRIIITTTNADLL-SNELGVKRIFEMEELK 370
KK L+VLDD++ D + L + + FGNGSRIIITT + L+ N++ I+E+ L
Sbjct: 302 KKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDI----IYEVTALP 361
Query: 371 YGEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIE 430
E++QL AF + P E + + ++V G PLALK+ GS L N L+ W IE
Sbjct: 362 DHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIE 421
Query: 431 EVEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTR 490
++ N I LK+SYDGL+ ++E+FLD+ACF +G+ ++ + +IL C A+
Sbjct: 422 HMK--NNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYG 481
Query: 491 VELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKH---VRDRLWCLKDIKSVVTE---- 550
+ +LI KSL+ +S N++QMHDL+Q+MG+ IV + R RLW K+++ V++
Sbjct: 482 LRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQKDPGERSRLWLAKEVEEVMSNNTGT 541
Query: 551 ALVQSIFLKSSKRNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYP 610
+++I++ S + M +LR+ N ++Y +P+ LR YP
Sbjct: 542 MAMEAIWVSSYSSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDY--LPNNLRCFVCTNYP 601
Query: 611 LEFLPFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNL 670
E P S+ E L+ L + H++L+ W + KHL L+ I L+ S+ L++TP+F +PNL
Sbjct: 602 WESFP-STFELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNL 661
Query: 671 KRLELEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKK 730
+ + L C++L +H S+ K+I L L C +L P +N++ LE L L C ++K
Sbjct: 662 EYVNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFPC-VNVESLEYLGLRSCDSLEK 721
Query: 731 IPEFSGNTNRLLQLHLDATSISNLPSSIASL-NHLTILSLNNCRKLINISNAIDKMTSLQ 790
+PE G +Q+H+ + I LPSSI H+T L L N + L+ + ++I ++ SL
Sbjct: 722 LPEIYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKSLV 781
Query: 791 SLDLSGCSKLGNRKRKADDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGI-F 850
SL +SGCSKL + + D++ + R K+ + + + G+ F
Sbjct: 782 SLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIRLNKLIILMFRGFKDGVHF 841
Query: 851 GIPSLA-GLYSLTRLNLRDCNLEE--IPQGIECLVSLVELNLSGNNFSQLPKSISRLHNL 910
P +A GL+SL LNL CNL + +P+ I L SL +L+LS NNF LP SI++L L
Sbjct: 842 EFPPVAEGLHSLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSRNNFEHLPSSIAQLGAL 901
Query: 911 KRLNINQCKKLVHFPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLAD 970
+ L++ C++L PELPP L + DC F+ Y + + L+ +L D
Sbjct: 902 QSLDLKDCQRLTQLPELPPE-LNELHVDCHMALKFI-------HYLVTKRKKLHRVKLDD 961
Query: 971 --NKGLHRLI-------ISWMQKMLFRKGTFNIMI----PGSE-IPDWFTTTKMGSSVCV 1020
N ++ L IS M+ + + ++ + P E IP WF SSV V
Sbjct: 962 AHNDTMYNLFAYTMFQNISSMRHDISASDSLSLTVFTGQPYPEKIPSWFHHQGWDSSVSV 1020
BLAST of Lsi08G001160 vs. ExPASy Swiss-Prot
Match:
V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)
HSP 1 Score: 556.6 bits (1433), Expect = 7.5e-157
Identity = 389/970 (40.10%), Postives = 539/970 (55.57%), Query Frame = 0
Query: 4 SSSSSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTD 63
SSSSSSSS+ S YDVFLSFRGEDTR FTDHLY AL +GI TFRD D++ G
Sbjct: 10 SSSSSSSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRD-DKLRRGEA 69
Query: 64 ISSDLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDR---MTVLPIFYKVDPS 123
I+ +LL AIE SR +V+V SENYA SRWCL ELVKI EC K + V PIFY VDPS
Sbjct: 70 IAPELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDPS 129
Query: 124 HVRKQSGSFAQAFLKHEIRFGED-DDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEIT 183
HVRKQ GSF +AF +GE+ D + +WR LT+ ANL W Q +ES I+EIT
Sbjct: 130 HVRKQEGSFGEAF----AGYGENLKDKIPRWRTALTEAANLSGWPLQD-GYESNQIKEIT 189
Query: 184 TTIWKRMKPSLTVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIA 243
+I++R+K + LVGI+ + E+ L+ S DV VG++G+GGIGKTTIA
Sbjct: 190 DSIFRRLK-CKRLDAGANLVGIDSHVKEMIWRLHMES---SDVRMVGMYGVGGIGKTTIA 249
Query: 244 RVCYERIRDEFEAHCFLSDVRENFETSGLPYLQTKLLSRMFSFK-NNDIWDVEEGIAMIN 303
+V Y + EFE FL ++RE F T G+ LQ +LL + + + +I V G +MI
Sbjct: 250 KVIYNELSREFEYMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIK 309
Query: 304 KAIFRKKALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEME 363
+ K +VLDDV+ Q+ L+ ++ G GSR+IITT N +L + V ++E++
Sbjct: 310 DILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQ-KVDDLYEVK 369
Query: 364 ELKYGEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWND 423
L + EA +L +L AF + PK Y ++V G PLALK+LG L K + W
Sbjct: 370 GLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWES 429
Query: 424 VIEEVEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYA 483
+ +++ +I S LK SYDGL EK IFLDVACFFKG+ R+ V +IL+ C F+A
Sbjct: 430 ELRKLD--REPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHA 489
Query: 484 KTRVELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHVRD-----RLWCLKDIKSVVT 543
+ ++ L K L+TL Y N+++MHDL+Q+MG +IVR K + RLW D + +T
Sbjct: 490 EIGIKNLNDKCLITLQY-NRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALT 549
Query: 544 E----ALVQSIFLKSSK-RNMVQFPILFSRMHQLRLLNFNN----------VGLKNKLE- 603
V++I L SK + + F++M +LRLL + + +K+E
Sbjct: 550 AYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVEL 609
Query: 604 YCII---------------PS-ELRYLKWKGYPLEFLPFSSSEEYKLIELHMCHSNLKQF 663
Y ++ PS ELRYL+W GYPL+FLP S+ + KL+ELH+ SN+KQ
Sbjct: 610 YDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLP-SNFDGGKLVELHLKCSNIKQL 669
Query: 664 WQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHPSIFTAEKLIFL 723
K LE LK I L++S+ LS+ F+ +PNL+RL L GC SL++IHPS+ +KL L
Sbjct: 670 RLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTL 729
Query: 724 SLKGCINLTNLPSHI-NIKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHLDATSISNLPS 783
SLK C L NLP I +++ LE+L L+ CSK +K PE GN L +L L T+I +LP
Sbjct: 730 SLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKDLPD 789
Query: 784 SIASLNHLTILSLNNCRK-----------------------LINISNAIDKMTSLQSLDL 843
SI L L L L++C K + ++ ++I + SL+ L L
Sbjct: 790 SIGDLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDLRNTAIKDLPDSIRDLESLERLYL 849
Query: 844 SGCSKLGNRKRKADDVE-LMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPS 903
S CSK K +++ LME D++ TA + D S
Sbjct: 850 SYCSKFEKFPEKGGNMKSLMELDLQNTAIKDLPD-------------------------S 909
Query: 904 LAGLYSLTRLNLRDCN-LEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNIN 906
+ L SL L+L +C+ E+ P+ + SL EL L LP SI L +L LN++
Sbjct: 910 IGDLESLKYLDLSNCSKFEKFPEKGGNMKSLTELFLENTAIKDLPDSIGDLESLVSLNLS 939
BLAST of Lsi08G001160 vs. ExPASy Swiss-Prot
Match:
V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)
HSP 1 Score: 544.3 bits (1401), Expect = 3.9e-153
Identity = 378/963 (39.25%), Postives = 531/963 (55.14%), Query Frame = 0
Query: 6 SSSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDIS 65
+SSSSS+ S YDVFLSFRGEDTR FTDHLY AL +GI TFRD D + G I+
Sbjct: 9 ASSSSSTPSIPRTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRD-DRLRRGEAIA 68
Query: 66 SDLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQ 125
+LL AIE SR +V+V SENYA SRWCL ELVKI EC+K V PIFY VDPSHVRKQ
Sbjct: 69 PELLKAIEESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQ 128
Query: 126 SGSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKR 185
GSF +AF +E + D + +WR LT+ ANL W +ES I+EIT +I+++
Sbjct: 129 EGSFGEAFAGYEENW---KDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQ 188
Query: 186 MKPSLTVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYER 245
+K V + LVGI+ + E+ L+ S DV VGI+G+GGIGKTTIA+V Y
Sbjct: 189 LKCKRLDVGAN-LVGIDSHVKEMILRLHLES---SDVRMVGIYGVGGIGKTTIAKVIYNE 248
Query: 246 IRDEFEAHCFLSDVRENFETSGLPYLQTKLLSRMFSFK-NNDIWDVEEGIAMINKAIFRK 305
+ EFE FL ++RE L +LQ +LL + + + +I V +MI + +
Sbjct: 249 LSCEFEYMSFLENIREGSNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLSR 308
Query: 306 KALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGE 365
+ +VLDDV+ Q+ L+ ++ G GSR+IITT N +L+ + V ++E+E L + E
Sbjct: 309 RVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQ-EVDDLYEVEGLNFEE 368
Query: 366 ALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVE 425
A +L +L AF + PK Y ++V G PLALK+LGS L K + W +++++
Sbjct: 369 ACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLD 428
Query: 426 GGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVEL 485
+ I LK SYDGLD +K IFLD+ACFFKG+ R+ V IL+GC F A+T +
Sbjct: 429 SEPKAD--IHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISN 488
Query: 486 LIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHK-----HVRDRLWCLKDIKSVVTE----A 545
L L+TL Y N++ MHDL+Q+MG +IVR + RLW D + +T
Sbjct: 489 LNDLCLITLPY-NQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIK 548
Query: 546 LVQSIFLKSSK-RNMVQFPILFSRMHQLRLL--------------------NFNNVGLKN 605
V+++ L SK + + +F++M +LRLL +V +K+
Sbjct: 549 SVETMSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKD 608
Query: 606 KLEYCI-----IPS-ELRYLKWKGYPLEFLPFSSSEEYKLIELHMCHSNLKQFWQQEKHL 665
+ + PS ELRYL+W GYPL+ LP + + KL+ELH+ SN+KQ WQ K L
Sbjct: 609 ASKMQLGQSFKFPSYELRYLRWDGYPLDSLPL-NFDGGKLVELHLKCSNIKQLWQGHKDL 668
Query: 666 EELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHPSIFTAEKLIFLSLKGCIN 725
E LK I L++S+ LS+ F+ +PNL+RL L GC SL++IHPS+ +KL LSL+ C
Sbjct: 669 ERLKVIDLSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNK 728
Query: 726 LTNLPSHI-NIKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHLDATSISNLPSSIASLNH 785
L NLP I +++ LE L LS CSK +K PE GN L +L L T+I +LP SI L
Sbjct: 729 LKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLES 788
Query: 786 LTILSLNNCRK-----------------------LINISNAIDKMTSLQSLDLSGCSKLG 845
L L L+NC K + ++ ++I + SL+ L+LS C+K
Sbjct: 789 LESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLEILNLSDCAKFE 848
Query: 846 NRKRKADDVE-LMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAGLYSL 905
K +++ L E D++ TA + D S+ L SL
Sbjct: 849 KFPEKGGNMKSLKELDLQNTAIKDLPD-------------------------SIGDLKSL 908
BLAST of Lsi08G001160 vs. ExPASy Swiss-Prot
Match:
Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)
HSP 1 Score: 543.5 bits (1399), Expect = 6.6e-153
Identity = 415/1252 (33.15%), Postives = 619/1252 (49.44%), Query Frame = 0
Query: 13 SSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLAAI 72
SSS S ++DVFLSFRG DTR FT HL KAL+ +GI +F D D + G ++++ L I
Sbjct: 3 SSSPSSAEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID-DRLRRGDNLTA-LFDRI 62
Query: 73 EASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFAQA 132
E S+ A++V S NYA+S WCL+ELVKI EC ++ V+PIFYKVD S V KQ SFA
Sbjct: 63 EKSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVP 122
Query: 133 FLKHEIRF-GEDDDNVKQWRRLLTQLANLKAWLSQSWS-HESKIIEEITTTIWKRMKPSL 192
F E+ F G + + W+ L +N+ ++ + S E+K+++EI +K++ L
Sbjct: 123 FKLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN-DL 182
Query: 193 TVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEF 252
+ LVGI +L L LL + +D D V +GI GM GIGKTT+A Y R+R +F
Sbjct: 183 APSGNEGLVGIESRLKNLEKLL--SWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQF 242
Query: 253 EAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKALLVL 312
+ CFL+++REN SGL L KL S + + ++ +I + + K+ L+VL
Sbjct: 243 DGSCFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVL 302
Query: 313 DDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLN 372
DDV QI L+ + + GSRIIITT ++ L+ G R + + +L EAL+L +
Sbjct: 303 DDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLIETIKG--RKYVLPKLNDREALKLFS 362
Query: 373 LSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNIN 432
L+AF P + + ++ GHPLALK+LGS L + W ++ ++ +
Sbjct: 363 LNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLK--SRSH 422
Query: 433 EKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSL 492
I+ L+ SY+ L +K +FLD+ACFF+ + + V +LN G V+ L+ K L
Sbjct: 423 GDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCL 482
Query: 493 LTLSYDNKLQMHDLLQEMGRKI---VRHKHVRD---------------RLWCLKDIKSVV 552
+TLS DN+++MHD+LQ M ++I V +RD RLW +DI ++
Sbjct: 483 ITLS-DNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLL 542
Query: 553 TEAL----VQSIFLKSSK-RNMVQFPILFSRMHQLRLLNFNNVGLKNKLE---------- 612
TE L ++ IFL +SK R M F M+ L+ L + E
Sbjct: 543 TEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRG 602
Query: 613 YCIIPSELRYLKWKGYPLEFLPFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLN 672
+P+EL YL W GYPL+ +P + L++L + HS L++ W EK + LK++ L+
Sbjct: 603 LSFLPNELTYLHWHGYPLQSIPLDFDPK-NLVDLKLPHSQLEEIWDDEKDVGMLKWVDLS 662
Query: 673 HSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHIN 732
HS NL + A NL+RL LEGCTSL + +I EKLI+L+L+ C +L +LP I
Sbjct: 663 HSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIK 722
Query: 733 IKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCR 792
+ L+ LILSGCS +KK P S N LL LD T I +LP SI + L +L+L NC+
Sbjct: 723 TQSLQTLILSGCSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFRRLALLNLKNCK 782
Query: 793 KLINISNAIDKMTSLQSLDLSGCSKL---GNRKRKADDVELMEPDMRGTARRRRDDDDNN 852
KL ++S+ + K+ LQ L LSGCS+L K + +E++ D + +N
Sbjct: 783 KLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMPKMMHLSN 842
Query: 853 IFKKIFLWLCKAPASGIFGIPSLAGLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGN 912
I + +F +P G LT L L C+L ++P I L SL L LSGN
Sbjct: 843 IKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGN 902
Query: 913 NFSQLPKSISRLHNLKRLNINQCKKLVHFPELPPRILRLMSKDCISMKDF------LDIS 972
N LP+S ++L+NLK ++ CK L P LP + L + +C S++ L +
Sbjct: 903 NIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPLTPLTVG 962
Query: 973 KIDHSYFMI-EVNLLNCDQLADNKGLHR----LIISWMQKMLFR----KGTFNIMIPGSE 1032
+ HS F+ LN D A G R L+ + K +R + I P +E
Sbjct: 963 ERIHSMFIFSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEPLVGICYPATE 1022
Query: 1033 IPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGNDVVNVPSFAIIASVTGKD 1092
IP WF ++G S+ + P + N + AL VV D D S + KD
Sbjct: 1023 IPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSVKCCGNFENKD 1082
Query: 1093 ----RFDTNLNDGDLMVSGFTVSGMK-KLDHIWM-----FVLPRTRTLARKISKYKEIKF 1152
RFD L + + K DH++M F++ + Y + F
Sbjct: 1083 SSFTRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLVKNVHGESNSCC-YTKASF 1142
Query: 1153 RFLLEVNYSQSVTPNVKVKKCGVVGFSLQFLSSLFVVPYIPVHCSSNCDTTSHSIESTLM 1201
F + + ++ +V KCG+ +LM
Sbjct: 1143 EFYVTDDETRKKIETCEVIKCGM----------------------------------SLM 1202
BLAST of Lsi08G001160 vs. ExPASy Swiss-Prot
Match:
A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)
HSP 1 Score: 526.6 bits (1355), Expect = 8.4e-148
Identity = 349/945 (36.93%), Postives = 526/945 (55.66%), Query Frame = 0
Query: 12 SSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLAA 71
+SSS+ YDVFLSFRGEDTR F HL+ AL EKGI TF D+ E++ G ISS+L+ A
Sbjct: 3 TSSSHHGRSYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKA 62
Query: 72 IEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFAQ 131
I SRFAVVV S+NYASS WCL+ELVKI E ++ + V+P+FY VDPS VRKQ+G +A
Sbjct: 63 IGESRFAVVVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAV 122
Query: 132 AFLKHEIRFGEDDDNVKQWRRLLTQLANLKAW-LSQSWS-HESKIIEEITTTIWKRMKPS 191
F K E +D D V +WR LT++AN+ L +++ ESK I++I I+ + S
Sbjct: 123 CFTKFEANLVDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFS 182
Query: 192 LTVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDE 251
+++ D LVGI ++ +LSSLL D V VGI GMGG+GKTT AR + R
Sbjct: 183 ISITNRD-LVGIESQIKKLSSLLRM---DLKGVRLVGIWGMGGVGKTTAARALFNRYYQN 242
Query: 252 FEAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKALLV 311
FE+ CFL DV+E + L YLQ LLS++ + D D EE ++ + + KK L+V
Sbjct: 243 FESACFLEDVKEYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVV 302
Query: 312 LDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLL 371
LDDV +DQ+ L+ ++ FG+GSRI+ITT + LL N V +E++ L+ EA++L
Sbjct: 303 LDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNH-DVHETYEIKVLEKDEAIELF 362
Query: 372 NLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNI 431
NL AF P++ + E +V GG PLALK+LGS L + L VW I+ ++ N
Sbjct: 363 NLHAFKRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLK--DNP 422
Query: 432 NEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKS 491
+I + LK+S+DGL ++EK IFLD+ACFF+G + + + + GF+ V+ L++KS
Sbjct: 423 EGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKS 482
Query: 492 LLTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVV-----TEALVQSIFLKS 551
L+ + ++K+QMHDL+QEMGR+I + R++ +D+K EA+ + +
Sbjct: 483 LIFI-LEDKIQMHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEP 542
Query: 552 SKRNMVQFPILFS-----RMHQLRLL--NFNNVGLKNKLEYCIIPSELRYLKWKGYPLEF 611
+ + ++S + +LR+L + N G + Y +P+ L +L+W+ Y
Sbjct: 543 EQFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAY--LPNSLLWLEWRNYSSNS 602
Query: 612 LPFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRL 671
P S+ E KL+ L M S++ + W K L L + L++ L +TP+F I NL+RL
Sbjct: 603 FP-SNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERL 662
Query: 672 ELEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPE 731
L C +LV +HPS+ + LI L++ CI+L LP+ I + LEVL L+ C +K PE
Sbjct: 663 ILSSCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPE 722
Query: 732 FSGNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDL 791
N L +L L +T I LP+SI L+ L L +++C +L+++ ++I + ++L +
Sbjct: 723 VERNMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSIWR---FRNLKI 782
Query: 792 SGCSKLGNRKRKADDVELMEPDMRGTARRRRD------------DDDNNIFKKIFLWLCK 851
S C KLG+ P++ G + R+ N+ FL +C
Sbjct: 783 SECEKLGSL-----------PEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEICN 842
Query: 852 APASGIFGIPSLAGLYSLTRLNLRDC----NLEEIP--------QGIECLVSLVE----- 906
S+ GL SLT L L DC NL IP G++ L++L +
Sbjct: 843 CKTISSLS-SSIWGLTSLTTLKLLDCRKLKNLPGIPNAINHLSGHGLQLLLTLEQPTIYE 902
BLAST of Lsi08G001160 vs. ExPASy TrEMBL
Match:
A0A1S3CBN9 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498654 PE=4 SV=1)
HSP 1 Score: 1853.6 bits (4800), Expect = 0.0e+00
Identity = 949/1112 (85.34%), Postives = 1015/1112 (91.28%), Query Frame = 0
Query: 7 SSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISS 66
SSSSSSSSS KWK+DVFLSFRGEDTRGGFTDHLYKAL +KGI TFRDE+EI+EG DISS
Sbjct: 4 SSSSSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIQEGEDISS 63
Query: 67 DLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQS 126
+LL +IEASRFA+VVVSENYASSRWCL+ELVKIFECE++ M VLPIFYKVDPSHVR Q
Sbjct: 64 NLLDSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDPSHVRNQR 123
Query: 127 GSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRM 186
G F +AF+KHE+RFG DD V++WRRLLT+LANLKAWLSQSWSHES IIEEITTTIWKR+
Sbjct: 124 GRFEEAFVKHEMRFGRDDIKVQKWRRLLTKLANLKAWLSQSWSHESNIIEEITTTIWKRL 183
Query: 187 KPSLTVVKEDQLVGINFKLNELSSLLNPNS--DDDDDVICVGIHGMGGIGKTTIARVCYE 246
K +LTV+KEDQLVGIN KLN+LSSLL PNS D+DDDVI VGIHGMGGIGKTTIA+VCY+
Sbjct: 184 KHNLTVIKEDQLVGINSKLNKLSSLLIPNSDEDEDDDVIFVGIHGMGGIGKTTIAKVCYQ 243
Query: 247 RIRDEFEAHCFLSDVREN-FETSG-LPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIF 306
RIRDEFEAHCFLSDVREN F TSG LPYLQTKLLSRMFSFKNN I DVEEGIAMINKAIF
Sbjct: 244 RIRDEFEAHCFLSDVRENYFRTSGDLPYLQTKLLSRMFSFKNNHILDVEEGIAMINKAIF 303
Query: 307 RKKALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKY 366
RKK LLVLDDV+ SDQIMGLIPNK+SFGNGSRIIITT NADLLSNE GVKRIFEM+ELKY
Sbjct: 304 RKKTLLVLDDVDCSDQIMGLIPNKSSFGNGSRIIITTRNADLLSNEFGVKRIFEMDELKY 363
Query: 367 GEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEE 426
EALQLL+LSAFM+ CPKEGYLEH KKIVKVVGGHPLALKLLGSSLRNK LSVWN+VIEE
Sbjct: 364 EEALQLLSLSAFMKTCPKEGYLEHSKKIVKVVGGHPLALKLLGSSLRNKNLSVWNEVIEE 423
Query: 427 VEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRV 486
VEGGGNI+EKIF CLKVSYDGLDEWEKEIFLDVACFF GKRREVVEEILNGCGFYAKTR+
Sbjct: 424 VEGGGNIHEKIFKCLKVSYDGLDEWEKEIFLDVACFFNGKRREVVEEILNGCGFYAKTRI 483
Query: 487 ELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQSIFL 546
ELLIQKSLLTLSYDNKL MHDLLQEMGRKIVR KHVRDRL C KDIKSVVTE LVQSIF
Sbjct: 484 ELLIQKSLLTLSYDNKLHMHDLLQEMGRKIVRDKHVRDRLMCHKDIKSVVTETLVQSIFF 543
Query: 547 KSSKRNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSS 606
KSS +NMV+FPILFSRMHQLRLLNF+NV LKNKLEYC IPSELRYLKWK YPLEFLP +S
Sbjct: 544 KSSSKNMVEFPILFSRMHQLRLLNFHNVRLKNKLEYC-IPSELRYLKWKEYPLEFLPINS 603
Query: 607 SEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGC 666
SEE KLIELHMCHSNLKQFWQQEK+L LKYIKLN SQ LSKTPNFA IPNLKRLELE C
Sbjct: 604 SEECKLIELHMCHSNLKQFWQQEKNLVGLKYIKLNSSQKLSKTPNFANIPNLKRLELEDC 663
Query: 667 TSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSGNT 726
TSLVNIHPSIFTAEKLIFL+LK CINLTNLPS INIKVLEVLILSGCSKVKK+PEFSGNT
Sbjct: 664 TSLVNIHPSIFTAEKLIFLNLKDCINLTNLPSRINIKVLEVLILSGCSKVKKVPEFSGNT 723
Query: 727 NRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGCSK 786
NRLLQLHLD TSISNLPSSIASL+HLTILSL NC+KLINISNA++ MTSLQSLD+SGC K
Sbjct: 724 NRLLQLHLDGTSISNLPSSIASLSHLTILSLANCKKLINISNAME-MTSLQSLDVSGCLK 783
Query: 787 LGNRKRKADDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAGLYS 846
LG+RKRKADD EL E D+R T RRRR+DD NNIFKKIFLWLCK PASGIFGIPSLAGLYS
Sbjct: 784 LGSRKRKADDGELGELDVRETTRRRRNDDSNNIFKKIFLWLCKTPASGIFGIPSLAGLYS 843
Query: 847 LTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCKKLVH 906
LT+LNLRDCNLEEIPQGIECLVSLVEL+LSGN+FS LP SISRLHNLK+L INQC KLV
Sbjct: 844 LTKLNLRDCNLEEIPQGIECLVSLVELDLSGNSFSHLPTSISRLHNLKKLRINQCIKLVQ 903
Query: 907 FPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLIISWMQ 966
FP+LPPRIL LMSKDCIS+KDF+DISK+D+ Y M EVNLLNC QLA+NKG HRLIISWMQ
Sbjct: 904 FPKLPPRILFLMSKDCISLKDFVDISKVDNLYIMKEVNLLNCYQLANNKGFHRLIISWMQ 963
Query: 967 KMLFRKGTFNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGND 1026
KMLFRKGTFNIMIPGSEIPDWFTT KMGSSVC+EWDP+ PN NMIRFALCVV GLS+ D
Sbjct: 964 KMLFRKGTFNIMIPGSEIPDWFTTRKMGSSVCIEWDPDGPNTNMIRFALCVVFGLSEKID 1023
Query: 1027 VVNVPSFAIIASVTGKDRFDTNL-NDGDLMVSGFTVSGMKKLDHIWMFVLPRTRTLARKI 1086
VVNVPSFAIIASVTGKDR D+NL N GDL++ GF V+GMKKLDHIWMFVLPRT TL RKI
Sbjct: 1024 VVNVPSFAIIASVTGKDRNDSNLKNGGDLLIGGFPVAGMKKLDHIWMFVLPRTGTLVRKI 1083
Query: 1087 SKYKEIKFRFLLE-VNYSQSVTPNVKVKKCGV 1113
S YKEIKFRFLL+ NY QS+TPNVKVK+CGV
Sbjct: 1084 SNYKEIKFRFLLQAANYRQSITPNVKVKECGV 1113
BLAST of Lsi08G001160 vs. ExPASy TrEMBL
Match:
A0A6J1I8R5 (TMV resistance protein N-like OS=Cucurbita maxima OX=3661 GN=LOC111472025 PE=4 SV=1)
HSP 1 Score: 1686.8 bits (4367), Expect = 0.0e+00
Identity = 850/1102 (77.13%), Postives = 960/1102 (87.11%), Query Frame = 0
Query: 11 SSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLA 70
SS SS KWK++VFLSFRGEDTR GFTD LY AL KGI TFRDEDEIEEG DIS+DL A
Sbjct: 4 SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGADISTDLSA 63
Query: 71 AIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFA 130
AIEASR A+VVVSENYASSRWCL+EL KIFEC R MTVLPIFYKVDPSHVRKQ+G+FA
Sbjct: 64 AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRKQTGTFA 123
Query: 131 QAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRMKPSL 190
+AF KHEIRFGE + N+++WR+LLT+LANLKAWL + W+HESK+IEEIT +WKR+KP+L
Sbjct: 124 EAFAKHEIRFGEHNPNIQKWRQLLTKLANLKAWLLEPWTHESKVIEEITKVLWKRIKPTL 183
Query: 191 TVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEF 250
V +E QLVGIN KL +LSSLLNPNS D+DVI +GIHGMGGIGKTT+ARVCYERIRD+F
Sbjct: 184 RVTQEHQLVGINSKLTKLSSLLNPNS--DEDVIWIGIHGMGGIGKTTLARVCYERIRDKF 243
Query: 251 EAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKALLVL 310
EAHCF+S+V+E FETSGLPYLQ++LLSRMFS +N DI DVEEGIAMIN+A+FRKK LLVL
Sbjct: 244 EAHCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVL 303
Query: 311 DDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLN 370
DDV SDQIMGLIPNK+SFGNGSRIIIT NADLLSNEL VKR+F+M EL EALQLLN
Sbjct: 304 DDVNCSDQIMGLIPNKDSFGNGSRIIITARNADLLSNELEVKRMFKMVELTNEEALQLLN 363
Query: 371 LSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNIN 430
LSA PK+ LEH K IVK+VGGHPLALKLLGSSLRNK L VW VIEE++GGGNI+
Sbjct: 364 LSA----SPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLKVWKYVIEELKGGGNIH 423
Query: 431 EKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSL 490
EK+F CLKVSYDGLDEWEKEIFLD+ACFFKGKR+E+VEEIL+ CGF+AK RVELLIQKSL
Sbjct: 424 EKVFKCLKVSYDGLDEWEKEIFLDIACFFKGKRKELVEEILHSCGFHAKIRVELLIQKSL 483
Query: 491 LTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQSIFLKSSKRNMV 550
LTLSY NKL MHDLLQEMG+KIVRHK ++DRLW KDIKS+V EA V+SI KS+ RN+V
Sbjct: 484 LTLSYHNKLMMHDLLQEMGQKIVRHKPIQDRLWRQKDIKSMVGEASVESILFKST-RNVV 543
Query: 551 QFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSSSEEYKLIE 610
+FPI FSRMHQLRLLNF+NV LKN LEYC IPSELRYLKWKGYPLE L +SEEYKLI+
Sbjct: 544 EFPISFSRMHQLRLLNFHNVRLKNVLEYC-IPSELRYLKWKGYPLESLVL-NSEEYKLIK 603
Query: 611 LHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHP 670
LHMCHS+LKQFW EKHLEELKYIKLNHSQ LSKTPNF IPNL RLEL GCTSLVNIHP
Sbjct: 604 LHMCHSHLKQFWHGEKHLEELKYIKLNHSQKLSKTPNFETIPNLTRLELVGCTSLVNIHP 663
Query: 671 SIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHL 730
+IFT+++L FLSLK CINLTN P INIK LEVLILSGCSK+KKIPEFSGNT++LL+LHL
Sbjct: 664 TIFTSKELTFLSLKDCINLTNFPPQINIKALEVLILSGCSKLKKIPEFSGNTDKLLELHL 723
Query: 731 DATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGCSKLGNRKRKA 790
D TSIS+LPSSIA L+HLT+LSL NC+ LINISNA+DKMTSL+SL+LSGCSKLGNRKRK
Sbjct: 724 DGTSISSLPSSIAILDHLTVLSLTNCKNLINISNALDKMTSLKSLNLSGCSKLGNRKRKR 783
Query: 791 DDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAGLYSLTRLNLRD 850
DVE +E D+R TA RR DD +NIF+KIFLWLCKAP SGIFGIPSLAGLYSLTRLNL D
Sbjct: 784 VDVETVELDVRRTA--RRTDDGDNIFRKIFLWLCKAPTSGIFGIPSLAGLYSLTRLNLSD 843
Query: 851 CNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCKKLVHFPELPPRI 910
C LEE+P+GIECLVSLV LNLS NNFS+LP SISRLHNLKRLN+N+C+KL+HFP+LPPRI
Sbjct: 844 CKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPKLPPRI 903
Query: 911 LRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLIISWMQKMLFRKGT 970
LRLMSK CIS+KDFLDISK+DHSYFMIE+NLLNC Q DNK LH+LI SWMQKMLFRKG
Sbjct: 904 LRLMSKGCISLKDFLDISKVDHSYFMIEMNLLNCFQWVDNKDLHKLITSWMQKMLFRKGA 963
Query: 971 FNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGNDVVNVPSFA 1030
FNI++PGSEIPDWFTT KMGSS+CVEWD + PNANM+RFALCV+CG S+ D+++VP
Sbjct: 964 FNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNANMVRFALCVICGPSNKQDILDVP-VT 1023
Query: 1031 IIASVTGKDRFDTNLNDGDLMVSGFTVSGMKKLDHIWMFVLPRTRTLARKISKYKEIKFR 1090
I ASVTGK+R D N N+GDL+V F VSGMKKLDHIWMFVLPRT TL RKI KEI+FR
Sbjct: 1024 IFASVTGKNRNDPNSNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKEIEFR 1083
Query: 1091 FLLEVNYSQSVTPNVKVKKCGV 1113
FLL+ NY+Q+V PNV++KKCGV
Sbjct: 1084 FLLQFNYNQTVIPNVELKKCGV 1093
BLAST of Lsi08G001160 vs. ExPASy TrEMBL
Match:
A0A6J1EBI7 (TMV resistance protein N-like OS=Cucurbita moschata OX=3662 GN=LOC111432675 PE=4 SV=1)
HSP 1 Score: 1682.9 bits (4357), Expect = 0.0e+00
Identity = 847/1102 (76.86%), Postives = 958/1102 (86.93%), Query Frame = 0
Query: 11 SSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLA 70
SS SS KWK++VFLSFRGEDTR GFTD LY AL KGI TFRDEDEIEEG+DIS+DL A
Sbjct: 4 SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGSDISTDLSA 63
Query: 71 AIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFA 130
AIEASR A+VVVSENYASSRWCL+EL KIFEC R MTVLPIFYKVDPSHVR Q+G+FA
Sbjct: 64 AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRNQTGTFA 123
Query: 131 QAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRMKPSL 190
+AF KH+ RFGE + N+++WR LLT+LANLKAWL + W+HESK+IEEITT +WKR+KP+L
Sbjct: 124 EAFAKHQHRFGEHNPNIQKWRHLLTKLANLKAWLLEPWTHESKVIEEITTVLWKRIKPTL 183
Query: 191 TVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEF 250
V +E QLVGIN KL +LSSLLNPNS D+DVI VGIHGMGGIGKTT+ARVCYERIRD+F
Sbjct: 184 IVTQEHQLVGINSKLTKLSSLLNPNS--DEDVIWVGIHGMGGIGKTTLARVCYERIRDKF 243
Query: 251 EAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKALLVL 310
EAHCF+S+V+E FETSGLPYLQ++LLSRMFS +N DI DVEEGIAMIN+A+FRKK LLVL
Sbjct: 244 EAHCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVL 303
Query: 311 DDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLN 370
DDV SDQIMGLIPNK+SFGNGSRIIITT NADLLSNE VKR+F+M EL EALQLLN
Sbjct: 304 DDVNCSDQIMGLIPNKDSFGNGSRIIITTRNADLLSNEFEVKRMFKMVELTNEEALQLLN 363
Query: 371 LSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNIN 430
L A CPK+ LEH K IVK+VGGHPLALKLLGSSLRNK L VW V+EE++GGGNI+
Sbjct: 364 LRA----CPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLRVWEYVMEEIKGGGNIH 423
Query: 431 EKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSL 490
EK+F CLKVSYDGLDEWEKEIFLDVACFFKGKR+ +VEEILNGCGF+AK RVELL+QKSL
Sbjct: 424 EKVFKCLKVSYDGLDEWEKEIFLDVACFFKGKRKGLVEEILNGCGFHAKIRVELLLQKSL 483
Query: 491 LTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQSIFLKSSKRNMV 550
LTLSY NKL +HDLLQEMGRKIVRHK ++DRLW KDIKS+V EA V+SI KS+ RN++
Sbjct: 484 LTLSYHNKLMVHDLLQEMGRKIVRHKPIQDRLWRQKDIKSMVGEASVESILFKST-RNVM 543
Query: 551 QFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSSSEEYKLIE 610
+FPI FSRMHQLRLLNF+NV LKN+LEYC IPSELRYLKWKGYPLE L +SEE KLI+
Sbjct: 544 EFPISFSRMHQLRLLNFHNVRLKNELEYC-IPSELRYLKWKGYPLENLVL-NSEECKLIK 603
Query: 611 LHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHP 670
LHMCHSNLKQFW EKHLEELKYIKLNHS LSKTPNF IPNL RLELEGCTSLVNIHP
Sbjct: 604 LHMCHSNLKQFWHGEKHLEELKYIKLNHSPKLSKTPNFETIPNLTRLELEGCTSLVNIHP 663
Query: 671 SIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHL 730
+IFTA+KL FLSLK CINLTN P INIK LE+LIL+GCSK+KKIPEFSGNT+ LL+LHL
Sbjct: 664 TIFTAKKLTFLSLKDCINLTNFPPQINIKALEILILNGCSKLKKIPEFSGNTDILLELHL 723
Query: 731 DATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGCSKLGNRKRKA 790
D TSIS+LPSSIA L+HLT+LSL NC+ LINISNA+DK+TSL+SL+LSGCSKLGNRKRK
Sbjct: 724 DGTSISSLPSSIAILDHLTVLSLTNCKNLINISNALDKITSLKSLNLSGCSKLGNRKRKR 783
Query: 791 DDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAGLYSLTRLNLRD 850
DVE +E D+R TA RR D+ +NIF+KI LWLCKAP SGIFGIPSLAGLYSLTRLNL D
Sbjct: 784 GDVETVEFDVRRTA--RRTDNGDNIFRKIILWLCKAPTSGIFGIPSLAGLYSLTRLNLSD 843
Query: 851 CNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCKKLVHFPELPPRI 910
C LEE+P+GIECLVSLV LNLS NNFS+LP SISRLHNLKRLN+N+C+KL+HFPELPPRI
Sbjct: 844 CKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPELPPRI 903
Query: 911 LRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLIISWMQKMLFRKGT 970
LRLMSK CIS+KDFLDISK+DHSYFMIE+NL+NC Q DNK L +LI SWMQKMLFRKG
Sbjct: 904 LRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCFQWVDNKELQKLITSWMQKMLFRKGA 963
Query: 971 FNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGNDVVNVPSFA 1030
FNI++PGSEIPDWFTT KMGSS+CVEWD + PNANM+RFALCV+CG S+ D+++VP F
Sbjct: 964 FNILVPGSEIPDWFTTRKMGSSICVEWDHDAPNANMVRFALCVICGPSNKKDILDVP-FT 1023
Query: 1031 IIASVTGKDRFDTNLNDGDLMVSGFTVSGMKKLDHIWMFVLPRTRTLARKISKYKEIKFR 1090
I ASVTGKDR D NLN+GDL+V F VSGMKKLDHIWMFVLPRT TL RKI KEI+FR
Sbjct: 1024 IFASVTGKDRNDPNLNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKEIEFR 1083
Query: 1091 FLLEVNYSQSVTPNVKVKKCGV 1113
FLL+ NY+Q+V PNV++KKCGV
Sbjct: 1084 FLLQFNYNQTVIPNVELKKCGV 1093
BLAST of Lsi08G001160 vs. ExPASy TrEMBL
Match:
A0A6J1CKI7 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012132 PE=4 SV=1)
HSP 1 Score: 1663.7 bits (4307), Expect = 0.0e+00
Identity = 855/1118 (76.48%), Postives = 966/1118 (86.40%), Query Frame = 0
Query: 6 SSSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKG-IFTFRDEDEIEEGTDI 65
S + SSSSSS K+KYDVFLSFRGEDTRGGFTDHLY ALK G IFTFRDE+EI++G DI
Sbjct: 5 SMAVSSSSSSTGKYKYDVFLSFRGEDTRGGFTDHLYAALKRNGIIFTFRDENEIQQGADI 64
Query: 66 SSDLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRK 125
SSDL AAIEASR AVVVVSENYASSRWCL EL++IFEC KR MTVLPIFYKVDPS VR
Sbjct: 65 SSDLSAAIEASRIAVVVVSENYASSRWCLGELLEIFECRKRLGMTVLPIFYKVDPSDVRN 124
Query: 126 QSGSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWK 185
Q GSFA+AF KH RFGE+D + +WR+ LTQLANLKAWLSQ WSHES+IIEEI+ +IWK
Sbjct: 125 QRGSFAEAFAKHGERFGEEDFRLTKWRQALTQLANLKAWLSQHWSHESEIIEEISASIWK 184
Query: 186 RMKPSLTVVKEDQLVGINFKLNELSSLL--NPNSDDDDDVICVGIHGMGGIGKTTIARVC 245
R+KP+LT+ +EDQL+GIN KL++LSSLL N NSDDDD V VGIHGMGGIGKTTIARV
Sbjct: 185 RIKPTLTLTREDQLLGINSKLDKLSSLLNRNSNSDDDDGVFWVGIHGMGGIGKTTIARVF 244
Query: 246 YERIRDEFEAHCFLSDVRENFETSGLPYLQTKLLSRM-FSFKNNDIWDVEEGIAMINKAI 305
YE+IRDEF+AHCFLSDVRE FETSGL YL +KLLSR+ FS KNNDIWD EEGIAMI++AI
Sbjct: 245 YEQIRDEFDAHCFLSDVREKFETSGLTYLHSKLLSRLTFSMKNNDIWDAEEGIAMISRAI 304
Query: 306 FRKKALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELK 365
F+KK LLVLDDV+ SDQI GLIP+ + FG GSRIIITT NADLLSNELGVKRI E+EELK
Sbjct: 305 FQKKTLLVLDDVDHSDQIKGLIPSNDFFGYGSRIIITTRNADLLSNELGVKRILEIEELK 364
Query: 366 YGEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIE 425
Y EALQ L+LS FM+ CP+EG+ EHCK I+K+VGGHPL LKLLG+ LRNK L VW+ VIE
Sbjct: 365 YEEALQHLSLSVFMKTCPEEGFXEHCKSIIKLVGGHPLGLKLLGTCLRNKDLGVWDCVIE 424
Query: 426 EVEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTR 485
E++ GGNI+ +IF CLKVSYDGLDE EK+IFLDVACFFKGKRR+ VEEIL+GCGFYA+ R
Sbjct: 425 ELQEGGNIDNQIFKCLKVSYDGLDEREKDIFLDVACFFKGKRRDFVEEILDGCGFYARRR 484
Query: 486 VELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHV-RDRLWCLKDIKSVVTEALVQSI 545
+ELLIQKSL+TLSYDNKLQMHDLLQEMGRKIVR K +DRLWC KDIK VVTEALVQSI
Sbjct: 485 IELLIQKSLITLSYDNKLQMHDLLQEMGRKIVREKPTQQDRLWCHKDIKCVVTEALVQSI 544
Query: 546 FLKSSKRNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPF 605
+ K S RN V+ PILFSRM QLRLLNF+NV L+N LEYC IP+ELRYLKWK YPLEFL
Sbjct: 545 YFK-SPRNTVEVPILFSRMQQLRLLNFHNVRLQNHLEYC-IPTELRYLKWKRYPLEFLRL 604
Query: 606 SSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELE 665
SSEEYKLI LH CHSNLKQFW EKHLE+LKYIKLNHSQ LSKTP+FAKIPNLKRLELE
Sbjct: 605 -SSEEYKLITLHXCHSNLKQFWPVEKHLEKLKYIKLNHSQKLSKTPDFAKIPNLKRLELE 664
Query: 666 GCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSG 725
GCTSLVNIHPSIF+AEKLIFLSL+ C NLTNLPSHINIKVLEVLILSGCSK+ K+PEFSG
Sbjct: 665 GCTSLVNIHPSIFSAEKLIFLSLRDCTNLTNLPSHINIKVLEVLILSGCSKLNKVPEFSG 724
Query: 726 NTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGC 785
NTNRLLQLH DATSI+NLPSS+ASLN LT+LSL NC+KL+NI + IDKMTSL+SL+LSGC
Sbjct: 725 NTNRLLQLHFDATSIANLPSSVASLNRLTVLSLKNCKKLVNICSVIDKMTSLESLNLSGC 784
Query: 786 SKLGNRKRKADDVE--LMEPDMRGTARRRRDDDDN---NIFKKIFLWLCKAPASGIFGIP 845
SKLGNRKRK DD L E D+RG A RRRDD+ NIFK++FLWLCKAP SGIFG+P
Sbjct: 785 SKLGNRKRKIDDNVGCLAELDVRG-APRRRDDEGKVGVNIFKQLFLWLCKAPESGIFGVP 844
Query: 846 SLAGLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNIN 905
SLAGLYSLT+LNL+DCNLE IPQGIECLVSL EL L GNNFS LP SISRLHNL+RLNIN
Sbjct: 845 SLAGLYSLTKLNLKDCNLETIPQGIECLVSLTELRLCGNNFSHLPTSISRLHNLRRLNIN 904
Query: 906 QCKKLVHFPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHR 965
CKKL+ FPELPPRI LMSK CIS+K+ +ISK+DHSYFM EVNLLNC QLADN GLHR
Sbjct: 905 GCKKLLCFPELPPRIRWLMSKGCISLKNIPNISKVDHSYFMREVNLLNCYQLADNIGLHR 964
Query: 966 LIISWMQKMLFRKGTFNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVC 1025
LI WM+KMLFRKGTFNIMIPGS+IPDWFT T+MGSS+CVEWDP+ NAN+IRFALCVVC
Sbjct: 965 LITYWMEKMLFRKGTFNIMIPGSQIPDWFTMTEMGSSICVEWDPDAANANLIRFALCVVC 1024
Query: 1026 GLSDGNDVVNVPSFAIIASVTGKDRFDTNLNDGDLMVSGFTVSGMKKLDHIWMFVLPRTR 1085
G ++ D+++ P FAIIASVTG+ D LN+GDL+V+ F VSGM+KLDHIW+FVLPRT+
Sbjct: 1025 GPTNQGDIIDTP-FAIIASVTGRGADDPCLNNGDLIVNAFNVSGMRKLDHIWLFVLPRTK 1084
Query: 1086 TLARKISKYKEIKFRFLLEVNYSQSV-TPNVKVKKCGV 1113
+LARKIS KEIKF+FL++VNYS+S +P++ +KKCGV
Sbjct: 1085 SLARKISTCKEIKFQFLVQVNYSRSASSPSIALKKCGV 1117
BLAST of Lsi08G001160 vs. ExPASy TrEMBL
Match:
A0A6J1CL32 (TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012132 PE=4 SV=1)
HSP 1 Score: 1596.3 bits (4132), Expect = 0.0e+00
Identity = 830/1118 (74.24%), Postives = 939/1118 (83.99%), Query Frame = 0
Query: 6 SSSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKG-IFTFRDEDEIEEGTDI 65
S + SSSSSS K+KYDVFLSFRGEDTRGGFTDHLY ALK G IFTFRDE+EI++G DI
Sbjct: 5 SMAVSSSSSSTGKYKYDVFLSFRGEDTRGGFTDHLYAALKRNGIIFTFRDENEIQQGADI 64
Query: 66 SSDLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRK 125
SSDL AAIEASR AVVVVSENYASSRWCL EL++IFEC KR MTVLPIFYKVDPS VR
Sbjct: 65 SSDLSAAIEASRIAVVVVSENYASSRWCLGELLEIFECRKRLGMTVLPIFYKVDPSDVRN 124
Query: 126 QSGSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWK 185
Q GSFA+AF KH RFGE+D + +WR+ LTQLANLKAWLSQ WSHES+IIEEI+ +IWK
Sbjct: 125 QRGSFAEAFAKHGERFGEEDFRLTKWRQALTQLANLKAWLSQHWSHESEIIEEISASIWK 184
Query: 186 RMKPSLTVVKEDQLVGINFKLNELSSLL--NPNSDDDDDVICVGIHGMGGIGKTTIARVC 245
R+KP+LT+ +EDQL+GIN KL++LSSLL N NSDDDD V VGIHGMGGIGKTTIARV
Sbjct: 185 RIKPTLTLTREDQLLGINSKLDKLSSLLNRNSNSDDDDGVFWVGIHGMGGIGKTTIARVF 244
Query: 246 YERIRDEFEAHCFLSDVRENFETSGLPYLQTKLLSRM-FSFKNNDIWDVEEGIAMINKAI 305
YE+IRDEF+AHCFLSDVRE FETSGL YL +KLLSR+ FS KNNDIWD EEGIAMI++AI
Sbjct: 245 YEQIRDEFDAHCFLSDVREKFETSGLTYLHSKLLSRLTFSMKNNDIWDAEEGIAMISRAI 304
Query: 306 FRKKALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELK 365
F+KK LLVLDDV+ SDQI GLIP+ + FG GSRIIITT NADLLSNELGVKRI E+EELK
Sbjct: 305 FQKKTLLVLDDVDHSDQIKGLIPSNDFFGYGSRIIITTRNADLLSNELGVKRILEIEELK 364
Query: 366 YGEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIE 425
Y EALQ L+LS FM+ CP+EG+ EHCK I+K+VGGHPL LKLLG+ LRNK L VW+ VIE
Sbjct: 365 YEEALQHLSLSVFMKTCPEEGFXEHCKSIIKLVGGHPLGLKLLGTCLRNKDLGVWDCVIE 424
Query: 426 EVEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTR 485
E++ GGNI+ +IF CLKVSYDGLDE EK+IFLDVACFFKGKRR+ VEEIL+GCGFYA+ R
Sbjct: 425 ELQEGGNIDNQIFKCLKVSYDGLDEREKDIFLDVACFFKGKRRDFVEEILDGCGFYARRR 484
Query: 486 VELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHV-RDRLWCLKDIKSVVTEALVQSI 545
+ELLIQKSL+TLSYDNKLQMHDLLQEMGRKIVR K +DRLWC KDIK VVTEALVQSI
Sbjct: 485 IELLIQKSLITLSYDNKLQMHDLLQEMGRKIVREKPTQQDRLWCHKDIKCVVTEALVQSI 544
Query: 546 FLKSSKRNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPF 605
+ K S RN V+ PILFSRM QLRLLNF+NV L+N LEYC IP+ELRYLKWK YPLEFL
Sbjct: 545 YFK-SPRNTVEVPILFSRMQQLRLLNFHNVRLQNHLEYC-IPTELRYLKWKRYPLEFLRL 604
Query: 606 SSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELE 665
SSEEYKLI LH CHSNLKQFW EKHLE+LKYIKLNHSQ LSKTP+FAKIPNLKRLELE
Sbjct: 605 -SSEEYKLITLHXCHSNLKQFWPVEKHLEKLKYIKLNHSQKLSKTPDFAKIPNLKRLELE 664
Query: 666 GCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSG 725
GCTSLVNIHPSIF+AEKLIFLSL+ C NLTNLPSHINIKVLEVLILSGCSK+ K+PEFSG
Sbjct: 665 GCTSLVNIHPSIFSAEKLIFLSLRDCTNLTNLPSHINIKVLEVLILSGCSKLNKVPEFSG 724
Query: 726 NTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGC 785
NTNRLLQLH DATSI+NLPSS+ASLN LT+LSL NC+KL+NI + IDKMTSL+SL+LSGC
Sbjct: 725 NTNRLLQLHFDATSIANLPSSVASLNRLTVLSLKNCKKLVNICSVIDKMTSLESLNLSGC 784
Query: 786 SKLGNRKRKADDVE--LMEPDMRGTARRRRDDDDN---NIFKKIFLWLCKAPASGIFGIP 845
SKLGNRKRK DD L E D+RG A RRRDD+ NIFK++FLWLCKAP SGIFG+P
Sbjct: 785 SKLGNRKRKIDDNVGCLAELDVRG-APRRRDDEGKVGVNIFKQLFLWLCKAPESGIFGVP 844
Query: 846 SLAGLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNIN 905
SLA EL L GNNFS LP SISRLHNL+RLNIN
Sbjct: 845 SLA-----------------------------ELRLCGNNFSHLPTSISRLHNLRRLNIN 904
Query: 906 QCKKLVHFPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHR 965
CKKL+ FPELPPRI LMSK CIS+K+ +ISK+DHSYFM EVNLLNC QLADN GLHR
Sbjct: 905 GCKKLLCFPELPPRIRWLMSKGCISLKNIPNISKVDHSYFMREVNLLNCYQLADNIGLHR 964
Query: 966 LIISWMQKMLFRKGTFNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVC 1025
LI WM+KMLFRKGTFNIMIPGS+IPDWFT T+MGSS+CVEWDP+ NAN+IRFALCVVC
Sbjct: 965 LITYWMEKMLFRKGTFNIMIPGSQIPDWFTMTEMGSSICVEWDPDAANANLIRFALCVVC 1024
Query: 1026 GLSDGNDVVNVPSFAIIASVTGKDRFDTNLNDGDLMVSGFTVSGMKKLDHIWMFVLPRTR 1085
G ++ D+++ P FAIIASVTG+ D LN+GDL+V+ F VSGM+KLDHIW+FVLPRT+
Sbjct: 1025 GPTNQGDIIDTP-FAIIASVTGRGADDPCLNNGDLIVNAFNVSGMRKLDHIWLFVLPRTK 1084
Query: 1086 TLARKISKYKEIKFRFLLEVNYSQSV-TPNVKVKKCGV 1113
+LARKIS KEIKF+FL++VNYS+S +P++ +KKCGV
Sbjct: 1085 SLARKISTCKEIKFQFLVQVNYSRSASSPSIALKKCGV 1088
BLAST of Lsi08G001160 vs. NCBI nr
Match:
XP_008459550.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo])
HSP 1 Score: 1853.6 bits (4800), Expect = 0.0e+00
Identity = 949/1112 (85.34%), Postives = 1015/1112 (91.28%), Query Frame = 0
Query: 7 SSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISS 66
SSSSSSSSS KWK+DVFLSFRGEDTRGGFTDHLYKAL +KGI TFRDE+EI+EG DISS
Sbjct: 4 SSSSSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIQEGEDISS 63
Query: 67 DLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQS 126
+LL +IEASRFA+VVVSENYASSRWCL+ELVKIFECE++ M VLPIFYKVDPSHVR Q
Sbjct: 64 NLLDSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDPSHVRNQR 123
Query: 127 GSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRM 186
G F +AF+KHE+RFG DD V++WRRLLT+LANLKAWLSQSWSHES IIEEITTTIWKR+
Sbjct: 124 GRFEEAFVKHEMRFGRDDIKVQKWRRLLTKLANLKAWLSQSWSHESNIIEEITTTIWKRL 183
Query: 187 KPSLTVVKEDQLVGINFKLNELSSLLNPNS--DDDDDVICVGIHGMGGIGKTTIARVCYE 246
K +LTV+KEDQLVGIN KLN+LSSLL PNS D+DDDVI VGIHGMGGIGKTTIA+VCY+
Sbjct: 184 KHNLTVIKEDQLVGINSKLNKLSSLLIPNSDEDEDDDVIFVGIHGMGGIGKTTIAKVCYQ 243
Query: 247 RIRDEFEAHCFLSDVREN-FETSG-LPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIF 306
RIRDEFEAHCFLSDVREN F TSG LPYLQTKLLSRMFSFKNN I DVEEGIAMINKAIF
Sbjct: 244 RIRDEFEAHCFLSDVRENYFRTSGDLPYLQTKLLSRMFSFKNNHILDVEEGIAMINKAIF 303
Query: 307 RKKALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKY 366
RKK LLVLDDV+ SDQIMGLIPNK+SFGNGSRIIITT NADLLSNE GVKRIFEM+ELKY
Sbjct: 304 RKKTLLVLDDVDCSDQIMGLIPNKSSFGNGSRIIITTRNADLLSNEFGVKRIFEMDELKY 363
Query: 367 GEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEE 426
EALQLL+LSAFM+ CPKEGYLEH KKIVKVVGGHPLALKLLGSSLRNK LSVWN+VIEE
Sbjct: 364 EEALQLLSLSAFMKTCPKEGYLEHSKKIVKVVGGHPLALKLLGSSLRNKNLSVWNEVIEE 423
Query: 427 VEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRV 486
VEGGGNI+EKIF CLKVSYDGLDEWEKEIFLDVACFF GKRREVVEEILNGCGFYAKTR+
Sbjct: 424 VEGGGNIHEKIFKCLKVSYDGLDEWEKEIFLDVACFFNGKRREVVEEILNGCGFYAKTRI 483
Query: 487 ELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQSIFL 546
ELLIQKSLLTLSYDNKL MHDLLQEMGRKIVR KHVRDRL C KDIKSVVTE LVQSIF
Sbjct: 484 ELLIQKSLLTLSYDNKLHMHDLLQEMGRKIVRDKHVRDRLMCHKDIKSVVTETLVQSIFF 543
Query: 547 KSSKRNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSS 606
KSS +NMV+FPILFSRMHQLRLLNF+NV LKNKLEYC IPSELRYLKWK YPLEFLP +S
Sbjct: 544 KSSSKNMVEFPILFSRMHQLRLLNFHNVRLKNKLEYC-IPSELRYLKWKEYPLEFLPINS 603
Query: 607 SEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGC 666
SEE KLIELHMCHSNLKQFWQQEK+L LKYIKLN SQ LSKTPNFA IPNLKRLELE C
Sbjct: 604 SEECKLIELHMCHSNLKQFWQQEKNLVGLKYIKLNSSQKLSKTPNFANIPNLKRLELEDC 663
Query: 667 TSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSGNT 726
TSLVNIHPSIFTAEKLIFL+LK CINLTNLPS INIKVLEVLILSGCSKVKK+PEFSGNT
Sbjct: 664 TSLVNIHPSIFTAEKLIFLNLKDCINLTNLPSRINIKVLEVLILSGCSKVKKVPEFSGNT 723
Query: 727 NRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGCSK 786
NRLLQLHLD TSISNLPSSIASL+HLTILSL NC+KLINISNA++ MTSLQSLD+SGC K
Sbjct: 724 NRLLQLHLDGTSISNLPSSIASLSHLTILSLANCKKLINISNAME-MTSLQSLDVSGCLK 783
Query: 787 LGNRKRKADDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAGLYS 846
LG+RKRKADD EL E D+R T RRRR+DD NNIFKKIFLWLCK PASGIFGIPSLAGLYS
Sbjct: 784 LGSRKRKADDGELGELDVRETTRRRRNDDSNNIFKKIFLWLCKTPASGIFGIPSLAGLYS 843
Query: 847 LTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCKKLVH 906
LT+LNLRDCNLEEIPQGIECLVSLVEL+LSGN+FS LP SISRLHNLK+L INQC KLV
Sbjct: 844 LTKLNLRDCNLEEIPQGIECLVSLVELDLSGNSFSHLPTSISRLHNLKKLRINQCIKLVQ 903
Query: 907 FPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLIISWMQ 966
FP+LPPRIL LMSKDCIS+KDF+DISK+D+ Y M EVNLLNC QLA+NKG HRLIISWMQ
Sbjct: 904 FPKLPPRILFLMSKDCISLKDFVDISKVDNLYIMKEVNLLNCYQLANNKGFHRLIISWMQ 963
Query: 967 KMLFRKGTFNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGND 1026
KMLFRKGTFNIMIPGSEIPDWFTT KMGSSVC+EWDP+ PN NMIRFALCVV GLS+ D
Sbjct: 964 KMLFRKGTFNIMIPGSEIPDWFTTRKMGSSVCIEWDPDGPNTNMIRFALCVVFGLSEKID 1023
Query: 1027 VVNVPSFAIIASVTGKDRFDTNL-NDGDLMVSGFTVSGMKKLDHIWMFVLPRTRTLARKI 1086
VVNVPSFAIIASVTGKDR D+NL N GDL++ GF V+GMKKLDHIWMFVLPRT TL RKI
Sbjct: 1024 VVNVPSFAIIASVTGKDRNDSNLKNGGDLLIGGFPVAGMKKLDHIWMFVLPRTGTLVRKI 1083
Query: 1087 SKYKEIKFRFLLE-VNYSQSVTPNVKVKKCGV 1113
S YKEIKFRFLL+ NY QS+TPNVKVK+CGV
Sbjct: 1084 SNYKEIKFRFLLQAANYRQSITPNVKVKECGV 1113
BLAST of Lsi08G001160 vs. NCBI nr
Match:
XP_011656070.1 (TMV resistance protein N [Cucumis sativus])
HSP 1 Score: 1782.3 bits (4615), Expect = 0.0e+00
Identity = 915/1116 (81.99%), Postives = 988/1116 (88.53%), Query Frame = 0
Query: 4 SSSSSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTD 63
++SSSS SSS S KWK+DVFLSFRGEDTRGGFTDHLYKAL KGI TFRDE+EIEEG
Sbjct: 10 AASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEH 69
Query: 64 ISSDLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVR 123
I S+LLA+I+ASRFA+VVVSE+YASSRWCL+EL ++FEC+K VLPIFYKVDPSHV+
Sbjct: 70 IPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPSHVK 129
Query: 124 KQSGSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIW 183
QSG+F +AF+KHE RFG D V+ WR LT+LAN KAWLSQSWSHES IIEEITT IW
Sbjct: 130 NQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSWSHESNIIEEITTKIW 189
Query: 184 KRMKPSLTVVKEDQLVGINFKLNELSSLLNPNSDD---DDDVICVGIHGMGGIGKTTIAR 243
KR+KP+LTV+KEDQLVGIN K+N+LSSLL PNSDD DDDVI VGIHGMGGIGKTTIAR
Sbjct: 190 KRLKPNLTVIKEDQLVGINSKINKLSSLLIPNSDDDDADDDVIFVGIHGMGGIGKTTIAR 249
Query: 244 VCYERIRDEFEAHCFLSDVRENF--ETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMIN 303
VCYERIRDEFEAHCFLS+VREN+ L LQTKLLS MFS KNN I DVEEG AMIN
Sbjct: 250 VCYERIRDEFEAHCFLSNVRENYIRTLGNLSCLQTKLLSSMFSLKNNHIMDVEEGTAMIN 309
Query: 304 KAIFRKKALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEME 363
KAIFRKK LLVLDDV+ SDQI GLIP+ NSFGNGSR+IITT NAD LSNE GVKRIFEM+
Sbjct: 310 KAIFRKKTLLVLDDVDSSDQIKGLIPDNNSFGNGSRVIITTRNADFLSNEFGVKRIFEMD 369
Query: 364 ELKYGEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWND 423
ELKY EALQLL+LSAFM+ CPKEGYLEH KKIVKVVGGHPLALKLLGSSLRNK LSVWN+
Sbjct: 370 ELKYEEALQLLSLSAFMKTCPKEGYLEHSKKIVKVVGGHPLALKLLGSSLRNKNLSVWNE 429
Query: 424 VIEEVEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYA 483
VIEEV GGGNI+EKIF CLKVSYDGLDE E+EIFLDVACFF GKRREVVEEILNGCGFYA
Sbjct: 430 VIEEVGGGGNIHEKIFKCLKVSYDGLDEREREIFLDVACFFNGKRREVVEEILNGCGFYA 489
Query: 484 KTRVELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQ 543
KTR+ELLIQKSLLTLSYDNKL MH+LLQEMGRKIVR KHVRDRL C KDIKSVVTEAL+Q
Sbjct: 490 KTRIELLIQKSLLTLSYDNKLHMHNLLQEMGRKIVRDKHVRDRLMCHKDIKSVVTEALIQ 549
Query: 544 SIFLKSSKRNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFL 603
SIF KSS +NMV+FPILFSRMHQLRLLNF NV LKNKLEY IPSELRYLKWKGYPLEFL
Sbjct: 550 SIFFKSSSKNMVEFPILFSRMHQLRLLNFRNVRLKNKLEYS-IPSELRYLKWKGYPLEFL 609
Query: 604 PFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLE 663
P SSEE KLIELHMCHSNLKQFWQQEK+L ELKYIKLN SQ LSKTPNFA IPNLKRLE
Sbjct: 610 PIDSSEECKLIELHMCHSNLKQFWQQEKNLVELKYIKLNSSQKLSKTPNFANIPNLKRLE 669
Query: 664 LEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEF 723
LE CTSLVNIHPSIFTAEKLIFLSLK CINLTNLPSHINIKVLEVLILSGCSKVKK+PEF
Sbjct: 670 LEDCTSLVNIHPSIFTAEKLIFLSLKDCINLTNLPSHINIKVLEVLILSGCSKVKKVPEF 729
Query: 724 SGNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLS 783
SGNTNRLLQLHLD TSISNLPSSIASL+HLTILSL NC+ LI+ISNAI+ MTSLQSLD+S
Sbjct: 730 SGNTNRLLQLHLDGTSISNLPSSIASLSHLTILSLANCKMLIDISNAIE-MTSLQSLDVS 789
Query: 784 GCSKLGNRKRKADDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLA 843
GCSKLG+RK K D+VEL E ++R T RRRR+DD NNIFK+IFLWLC PA+GIFGIPSLA
Sbjct: 790 GCSKLGSRKGKGDNVELGEVNVRETTRRRRNDDCNNIFKEIFLWLCNTPATGIFGIPSLA 849
Query: 844 GLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCK 903
GLYSLT+LNL+DCNLE IPQGIEC+VSLVEL+LSGNNFS LP SISRLHNLKRL INQCK
Sbjct: 850 GLYSLTKLNLKDCNLEVIPQGIECMVSLVELDLSGNNFSHLPTSISRLHNLKRLRINQCK 909
Query: 904 KLVHFPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLII 963
KLVHFP+LPPRIL L SKDCIS+KDF+DISK+D+ Y M EVNLLNC Q+A+NK HRLII
Sbjct: 910 KLVHFPKLPPRILFLTSKDCISLKDFIDISKVDNLYIMKEVNLLNCYQMANNKDFHRLII 969
Query: 964 SWMQKMLFRKGTFNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLS 1023
S MQKM FRKGTFNIMIPGSEIPDWFTT KMGSSVC+EWDP+ PN NMIRFALCVV GLS
Sbjct: 970 SSMQKMFFRKGTFNIMIPGSEIPDWFTTRKMGSSVCMEWDPDAPNTNMIRFALCVVIGLS 1029
Query: 1024 DGNDVVNVPSFAIIASVTGKDRFDTNLNDG-DLMVSGFTVSGMKKLDHIWMFVLPRTRTL 1083
D +DV NV SF IIASVTGKDR DTNL +G DL+V GF VSGMKKLDHIWMFVLPRT TL
Sbjct: 1030 DKSDVCNVSSFTIIASVTGKDRNDTNLKNGDDLLVDGFLVSGMKKLDHIWMFVLPRTGTL 1089
Query: 1084 ARKISKYKEIKFRFLLE-VNYSQSVTPNVKVKKCGV 1113
RKIS YKEIKFRFLL+ NY QS+TPNV+VKKCGV
Sbjct: 1090 LRKISNYKEIKFRFLLQAANYRQSITPNVEVKKCGV 1119
BLAST of Lsi08G001160 vs. NCBI nr
Match:
KAE8648990.1 (hypothetical protein Csa_007990 [Cucumis sativus])
HSP 1 Score: 1756.1 bits (4547), Expect = 0.0e+00
Identity = 902/1103 (81.78%), Postives = 975/1103 (88.40%), Query Frame = 0
Query: 4 SSSSSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTD 63
++SSSS SSS S KWK+DVFLSFRGEDTRGGFTDHLYKAL KGI TFRDE+EIEEG
Sbjct: 10 AASSSSPSSSPSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTRKGISTFRDENEIEEGEH 69
Query: 64 ISSDLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVR 123
I S+LLA+I+ASRFA+VVVSE+YASSRWCL+EL ++FEC+K VLPIFYKVDPSHV+
Sbjct: 70 IPSNLLASIDASRFAIVVVSEDYASSRWCLEELARMFECKKE----VLPIFYKVDPSHVK 129
Query: 124 KQSGSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIW 183
QSG+F +AF+KHE RFG D V+ WR LT+LAN KAWLSQSWSHES IIEEITT IW
Sbjct: 130 NQSGTFEEAFVKHEKRFGRGDGKVQSWRTFLTELANTKAWLSQSWSHESNIIEEITTKIW 189
Query: 184 KRMKPSLTVVKEDQLVGINFKLNELSSLLNPNSDD---DDDVICVGIHGMGGIGKTTIAR 243
KR+KP+LTV+KEDQLVGIN K+N+LSSLL PNSDD DDDVI VGIHGMGGIGKTTIAR
Sbjct: 190 KRLKPNLTVIKEDQLVGINSKINKLSSLLIPNSDDDDADDDVIFVGIHGMGGIGKTTIAR 249
Query: 244 VCYERIRDEFEAHCFLSDVRENF--ETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMIN 303
VCYERIRDEFEAHCFLS+VREN+ L LQTKLLS MFS KNN I DVEEG AMIN
Sbjct: 250 VCYERIRDEFEAHCFLSNVRENYIRTLGNLSCLQTKLLSSMFSLKNNHIMDVEEGTAMIN 309
Query: 304 KAIFRKKALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEME 363
KAIFRKK LLVLDDV+ SDQI GLIP+ NSFGNGSR+IITT NAD LSNE GVKRIFEM+
Sbjct: 310 KAIFRKKTLLVLDDVDSSDQIKGLIPDNNSFGNGSRVIITTRNADFLSNEFGVKRIFEMD 369
Query: 364 ELKYGEALQLLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWND 423
ELKY EALQLL+LSAFM+ CPKEGYLEH KKIVKVVGGHPLALKLLGSSLRNK LSVWN+
Sbjct: 370 ELKYEEALQLLSLSAFMKTCPKEGYLEHSKKIVKVVGGHPLALKLLGSSLRNKNLSVWNE 429
Query: 424 VIEEVEGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYA 483
VIEEV GGGNI+EKIF CLKVSYDGLDE E+EIFLDVACFF GKRREVVEEILNGCGFYA
Sbjct: 430 VIEEVGGGGNIHEKIFKCLKVSYDGLDEREREIFLDVACFFNGKRREVVEEILNGCGFYA 489
Query: 484 KTRVELLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQ 543
KTR+ELLIQKSLLTLSYDNKL MH+LLQEMGRKIVR KHVRDRL C KDIKSVVTEAL+Q
Sbjct: 490 KTRIELLIQKSLLTLSYDNKLHMHNLLQEMGRKIVRDKHVRDRLMCHKDIKSVVTEALIQ 549
Query: 544 SIFLKSSKRNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFL 603
SIF KSS +NMV+FPILFSRMHQLRLLNF NV LKNKLEY IPSELRYLKWKGYPLEFL
Sbjct: 550 SIFFKSSSKNMVEFPILFSRMHQLRLLNFRNVRLKNKLEYS-IPSELRYLKWKGYPLEFL 609
Query: 604 PFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLE 663
P SSEE KLIELHMCHSNLKQFWQQEK+L ELKYIKLN SQ LSKTPNFA IPNLKRLE
Sbjct: 610 PIDSSEECKLIELHMCHSNLKQFWQQEKNLVELKYIKLNSSQKLSKTPNFANIPNLKRLE 669
Query: 664 LEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEF 723
LE CTSLVNIHPSIFTAEKLIFLSLK CINLTNLPSHINIKVLEVLILSGCSKVKK+PEF
Sbjct: 670 LEDCTSLVNIHPSIFTAEKLIFLSLKDCINLTNLPSHINIKVLEVLILSGCSKVKKVPEF 729
Query: 724 SGNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLS 783
SGNTNRLLQLHLD TSISNLPSSIASL+HLTILSL NC+ LI+ISNAI+ MTSLQSLD+S
Sbjct: 730 SGNTNRLLQLHLDGTSISNLPSSIASLSHLTILSLANCKMLIDISNAIE-MTSLQSLDVS 789
Query: 784 GCSKLGNRKRKADDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLA 843
GCSKLG+RK K D+VEL E ++R T RRRR+DD NNIFK+IFLWLC PA+GIFGIPSLA
Sbjct: 790 GCSKLGSRKGKGDNVELGEVNVRETTRRRRNDDCNNIFKEIFLWLCNTPATGIFGIPSLA 849
Query: 844 GLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCK 903
GLYSLT+LNL+DCNLE IPQGIEC+VSLVEL+LSGNNFS LP SISRLHNLKRL INQCK
Sbjct: 850 GLYSLTKLNLKDCNLEVIPQGIECMVSLVELDLSGNNFSHLPTSISRLHNLKRLRINQCK 909
Query: 904 KLVHFPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLII 963
KLVHFP+LPPRIL L SKDCIS+KDF+DISK+D+ Y M EVNLLNC Q+A+NK HRLII
Sbjct: 910 KLVHFPKLPPRILFLTSKDCISLKDFIDISKVDNLYIMKEVNLLNCYQMANNKDFHRLII 969
Query: 964 SWMQKMLFRKGTFNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLS 1023
S MQKM FRKGTFNIMIPGSEIPDWFTT KMGSSVC+EWDP+ PN NMIRFALCVV GLS
Sbjct: 970 SSMQKMFFRKGTFNIMIPGSEIPDWFTTRKMGSSVCMEWDPDAPNTNMIRFALCVVIGLS 1029
Query: 1024 DGNDVVNVPSFAIIASVTGKDRFDTNLNDG-DLMVSGFTVSGMKKLDHIWMFVLPRTRTL 1083
D +DV NV SF IIASVTGKDR DTNL +G DL+V GF VSGMKKLDHIWMFVLPRT TL
Sbjct: 1030 DKSDVCNVSSFTIIASVTGKDRNDTNLKNGDDLLVDGFLVSGMKKLDHIWMFVLPRTGTL 1089
Query: 1084 ARKISKYKEIKFRFLLEVNYSQS 1101
RKIS YKEIKFRFLL+ ++S
Sbjct: 1090 LRKISNYKEIKFRFLLQAINNKS 1106
BLAST of Lsi08G001160 vs. NCBI nr
Match:
KAG6592347.1 (Disease resistance protein RUN1, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1696.8 bits (4393), Expect = 0.0e+00
Identity = 853/1102 (77.40%), Postives = 962/1102 (87.30%), Query Frame = 0
Query: 11 SSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLA 70
SS SS KWK++VFLSFRGEDTR GFTD LY AL KGI TFRDEDEIEEG+DIS+DL A
Sbjct: 4 SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGSDISTDLSA 63
Query: 71 AIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFA 130
AIEASR A+VVVSENYASSRWCL+EL KIFEC R MTVLPIFYKVDPSHVR Q+G+FA
Sbjct: 64 AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRNQTGTFA 123
Query: 131 QAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRMKPSL 190
+AF KHE+RFGE + N+++WR LLT+LANLKAWL + W+HESK+IEEITT +WKR+KP+L
Sbjct: 124 EAFAKHELRFGEHNPNIQKWRHLLTKLANLKAWLLEPWTHESKVIEEITTVLWKRIKPTL 183
Query: 191 TVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEF 250
V +E QLVGIN KL +LSSLLNPNS D+DVI VGIHGMGGIGKTT+ARVCYERIRD+F
Sbjct: 184 IVTQEHQLVGINSKLTKLSSLLNPNS--DEDVIWVGIHGMGGIGKTTLARVCYERIRDKF 243
Query: 251 EAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKALLVL 310
EAHCF+S+V+E FETSGLPYLQ++LLSRMFS +N DI DVEEGIAMIN+A+FRKK LLVL
Sbjct: 244 EAHCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVL 303
Query: 311 DDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLN 370
DDV SDQIMGLIPNK+SFGNGSRIIITT NADLLSNE VKR+F+M EL EALQLLN
Sbjct: 304 DDVNCSDQIMGLIPNKDSFGNGSRIIITTRNADLLSNEFEVKRMFKMVELTNEEALQLLN 363
Query: 371 LSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNIN 430
LSA CPK+ LEH K IVK+VGGHPLALKLLGSSLRNK L VW VIEE++GGGNI+
Sbjct: 364 LSA----CPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLKVWKYVIEELKGGGNIH 423
Query: 431 EKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSL 490
+K+F CLKVSYDGLDEWEKEIFLD+ACFFKGKR+E+VEEILNGCGF+AK RVELLIQKSL
Sbjct: 424 DKVFKCLKVSYDGLDEWEKEIFLDLACFFKGKRKELVEEILNGCGFHAKIRVELLIQKSL 483
Query: 491 LTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQSIFLKSSKRNMV 550
LTLSY NKL MHDLLQEMGRKIVRHK V++RLW KDIKS+V EA V+SI KS+ RN++
Sbjct: 484 LTLSYQNKLMMHDLLQEMGRKIVRHKPVQERLWRQKDIKSMVGEASVESILFKST-RNVM 543
Query: 551 QFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSSSEEYKLIE 610
+FPI FSRMHQLRLLNF+NV LKN+LEYC IPSELRYLKWKGYPLE L +SEE KLI+
Sbjct: 544 EFPISFSRMHQLRLLNFHNVRLKNELEYC-IPSELRYLKWKGYPLEILVL-NSEECKLIK 603
Query: 611 LHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHP 670
LHMCHSNLKQFW EKHLEELKYIKLNHS LSKTPNF IPNL RLELEGCTSLVNIHP
Sbjct: 604 LHMCHSNLKQFWHGEKHLEELKYIKLNHSPKLSKTPNFETIPNLTRLELEGCTSLVNIHP 663
Query: 671 SIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHL 730
+IFTA+KL FLSLK CINLTN P INIK LE+LIL+GCSK+KKIPEFSGNT+ LL+LHL
Sbjct: 664 TIFTAKKLTFLSLKDCINLTNFPPQINIKALEILILNGCSKLKKIPEFSGNTDILLELHL 723
Query: 731 DATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGCSKLGNRKRKA 790
D TSIS+LPSSIA L+HLT+LSL NC+ LINISNA+DK+TSL+SL+LSGCSKLGNRKRK
Sbjct: 724 DGTSISSLPSSIAILDHLTVLSLTNCKNLINISNALDKITSLKSLNLSGCSKLGNRKRKR 783
Query: 791 DDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAGLYSLTRLNLRD 850
DVE +E D+R TA RR DD +NIF+KI LWLCKAP SGIFGIPSLAGLYSLTRLNL D
Sbjct: 784 GDVETVEFDVRRTA--RRTDDGDNIFRKIILWLCKAPTSGIFGIPSLAGLYSLTRLNLSD 843
Query: 851 CNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCKKLVHFPELPPRI 910
C LEE+P+GIECLVSLV LNLS NNFS+LP SISRLHNLKRLN+N+C+KL+HFPELPPRI
Sbjct: 844 CKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPELPPRI 903
Query: 911 LRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLIISWMQKMLFRKGT 970
LRLMSK CIS+KDFLDISK+DHSYFMIE+NL+NC Q DNK LH+LI SWMQKMLFRKG
Sbjct: 904 LRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCFQWVDNKELHKLITSWMQKMLFRKGA 963
Query: 971 FNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGNDVVNVPSFA 1030
FNI++PGSEIPDWFTT KMGSS+CVEWD + PNANM+RFALCV+CG S+ D+++VP F
Sbjct: 964 FNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNANMVRFALCVICGPSNKKDILDVP-FT 1023
Query: 1031 IIASVTGKDRFDTNLNDGDLMVSGFTVSGMKKLDHIWMFVLPRTRTLARKISKYKEIKFR 1090
I ASVTGKDR D NLN+GDL+V F VSGMKKLDHIWMFVLPRT TL RKI KEI+FR
Sbjct: 1024 IFASVTGKDRNDPNLNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKEIEFR 1083
Query: 1091 FLLEVNYSQSVTPNVKVKKCGV 1113
FLL+ NY+Q+V PNV++KKCGV
Sbjct: 1084 FLLQFNYNQTVIPNVELKKCGV 1093
BLAST of Lsi08G001160 vs. NCBI nr
Match:
XP_022973476.1 (TMV resistance protein N-like [Cucurbita maxima])
HSP 1 Score: 1686.8 bits (4367), Expect = 0.0e+00
Identity = 850/1102 (77.13%), Postives = 960/1102 (87.11%), Query Frame = 0
Query: 11 SSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLA 70
SS SS KWK++VFLSFRGEDTR GFTD LY AL KGI TFRDEDEIEEG DIS+DL A
Sbjct: 4 SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGADISTDLSA 63
Query: 71 AIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFA 130
AIEASR A+VVVSENYASSRWCL+EL KIFEC R MTVLPIFYKVDPSHVRKQ+G+FA
Sbjct: 64 AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRKQTGTFA 123
Query: 131 QAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRMKPSL 190
+AF KHEIRFGE + N+++WR+LLT+LANLKAWL + W+HESK+IEEIT +WKR+KP+L
Sbjct: 124 EAFAKHEIRFGEHNPNIQKWRQLLTKLANLKAWLLEPWTHESKVIEEITKVLWKRIKPTL 183
Query: 191 TVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEF 250
V +E QLVGIN KL +LSSLLNPNS D+DVI +GIHGMGGIGKTT+ARVCYERIRD+F
Sbjct: 184 RVTQEHQLVGINSKLTKLSSLLNPNS--DEDVIWIGIHGMGGIGKTTLARVCYERIRDKF 243
Query: 251 EAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKALLVL 310
EAHCF+S+V+E FETSGLPYLQ++LLSRMFS +N DI DVEEGIAMIN+A+FRKK LLVL
Sbjct: 244 EAHCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVL 303
Query: 311 DDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLN 370
DDV SDQIMGLIPNK+SFGNGSRIIIT NADLLSNEL VKR+F+M EL EALQLLN
Sbjct: 304 DDVNCSDQIMGLIPNKDSFGNGSRIIITARNADLLSNELEVKRMFKMVELTNEEALQLLN 363
Query: 371 LSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNIN 430
LSA PK+ LEH K IVK+VGGHPLALKLLGSSLRNK L VW VIEE++GGGNI+
Sbjct: 364 LSA----SPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLKVWKYVIEELKGGGNIH 423
Query: 431 EKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSL 490
EK+F CLKVSYDGLDEWEKEIFLD+ACFFKGKR+E+VEEIL+ CGF+AK RVELLIQKSL
Sbjct: 424 EKVFKCLKVSYDGLDEWEKEIFLDIACFFKGKRKELVEEILHSCGFHAKIRVELLIQKSL 483
Query: 491 LTLSYDNKLQMHDLLQEMGRKIVRHKHVRDRLWCLKDIKSVVTEALVQSIFLKSSKRNMV 550
LTLSY NKL MHDLLQEMG+KIVRHK ++DRLW KDIKS+V EA V+SI KS+ RN+V
Sbjct: 484 LTLSYHNKLMMHDLLQEMGQKIVRHKPIQDRLWRQKDIKSMVGEASVESILFKST-RNVV 543
Query: 551 QFPILFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSSSEEYKLIE 610
+FPI FSRMHQLRLLNF+NV LKN LEYC IPSELRYLKWKGYPLE L +SEEYKLI+
Sbjct: 544 EFPISFSRMHQLRLLNFHNVRLKNVLEYC-IPSELRYLKWKGYPLESLVL-NSEEYKLIK 603
Query: 611 LHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHP 670
LHMCHS+LKQFW EKHLEELKYIKLNHSQ LSKTPNF IPNL RLEL GCTSLVNIHP
Sbjct: 604 LHMCHSHLKQFWHGEKHLEELKYIKLNHSQKLSKTPNFETIPNLTRLELVGCTSLVNIHP 663
Query: 671 SIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHL 730
+IFT+++L FLSLK CINLTN P INIK LEVLILSGCSK+KKIPEFSGNT++LL+LHL
Sbjct: 664 TIFTSKELTFLSLKDCINLTNFPPQINIKALEVLILSGCSKLKKIPEFSGNTDKLLELHL 723
Query: 731 DATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSGCSKLGNRKRKA 790
D TSIS+LPSSIA L+HLT+LSL NC+ LINISNA+DKMTSL+SL+LSGCSKLGNRKRK
Sbjct: 724 DGTSISSLPSSIAILDHLTVLSLTNCKNLINISNALDKMTSLKSLNLSGCSKLGNRKRKR 783
Query: 791 DDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAGLYSLTRLNLRD 850
DVE +E D+R TA RR DD +NIF+KIFLWLCKAP SGIFGIPSLAGLYSLTRLNL D
Sbjct: 784 VDVETVELDVRRTA--RRTDDGDNIFRKIFLWLCKAPTSGIFGIPSLAGLYSLTRLNLSD 843
Query: 851 CNLEEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQCKKLVHFPELPPRI 910
C LEE+P+GIECLVSLV LNLS NNFS+LP SISRLHNLKRLN+N+C+KL+HFP+LPPRI
Sbjct: 844 CKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPKLPPRI 903
Query: 911 LRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLHRLIISWMQKMLFRKGT 970
LRLMSK CIS+KDFLDISK+DHSYFMIE+NLLNC Q DNK LH+LI SWMQKMLFRKG
Sbjct: 904 LRLMSKGCISLKDFLDISKVDHSYFMIEMNLLNCFQWVDNKDLHKLITSWMQKMLFRKGA 963
Query: 971 FNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGNDVVNVPSFA 1030
FNI++PGSEIPDWFTT KMGSS+CVEWD + PNANM+RFALCV+CG S+ D+++VP
Sbjct: 964 FNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNANMVRFALCVICGPSNKQDILDVP-VT 1023
Query: 1031 IIASVTGKDRFDTNLNDGDLMVSGFTVSGMKKLDHIWMFVLPRTRTLARKISKYKEIKFR 1090
I ASVTGK+R D N N+GDL+V F VSGMKKLDHIWMFVLPRT TL RKI KEI+FR
Sbjct: 1024 IFASVTGKNRNDPNSNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKEIEFR 1083
Query: 1091 FLLEVNYSQSVTPNVKVKKCGV 1113
FLL+ NY+Q+V PNV++KKCGV
Sbjct: 1084 FLLQFNYNQTVIPNVELKKCGV 1093
BLAST of Lsi08G001160 vs. TAIR 10
Match:
AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 543.5 bits (1399), Expect = 4.7e-154
Identity = 415/1252 (33.15%), Postives = 619/1252 (49.44%), Query Frame = 0
Query: 13 SSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLAAI 72
SSS S ++DVFLSFRG DTR FT HL KAL+ +GI +F D D + G ++++ L I
Sbjct: 3 SSSPSSAEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID-DRLRRGDNLTA-LFDRI 62
Query: 73 EASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSGSFAQA 132
E S+ A++V S NYA+S WCL+ELVKI EC ++ V+PIFYKVD S V KQ SFA
Sbjct: 63 EKSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVP 122
Query: 133 FLKHEIRF-GEDDDNVKQWRRLLTQLANLKAWLSQSWS-HESKIIEEITTTIWKRMKPSL 192
F E+ F G + + W+ L +N+ ++ + S E+K+++EI +K++ L
Sbjct: 123 FKLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN-DL 182
Query: 193 TVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEF 252
+ LVGI +L L LL + +D D V +GI GM GIGKTT+A Y R+R +F
Sbjct: 183 APSGNEGLVGIESRLKNLEKLL--SWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQF 242
Query: 253 EAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKALLVL 312
+ CFL+++REN SGL L KL S + + ++ +I + + K+ L+VL
Sbjct: 243 DGSCFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVL 302
Query: 313 DDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLN 372
DDV QI L+ + + GSRIIITT ++ L+ G R + + +L EAL+L +
Sbjct: 303 DDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLIETIKG--RKYVLPKLNDREALKLFS 362
Query: 373 LSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNIN 432
L+AF P + + ++ GHPLALK+LGS L + W ++ ++ +
Sbjct: 363 LNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLK--SRSH 422
Query: 433 EKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSL 492
I+ L+ SY+ L +K +FLD+ACFF+ + + V +LN G V+ L+ K L
Sbjct: 423 GDIYEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCL 482
Query: 493 LTLSYDNKLQMHDLLQEMGRKI---VRHKHVRD---------------RLWCLKDIKSVV 552
+TLS DN+++MHD+LQ M ++I V +RD RLW +DI ++
Sbjct: 483 ITLS-DNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLL 542
Query: 553 TEAL----VQSIFLKSSK-RNMVQFPILFSRMHQLRLLNFNNVGLKNKLE---------- 612
TE L ++ IFL +SK R M F M+ L+ L + E
Sbjct: 543 TEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRG 602
Query: 613 YCIIPSELRYLKWKGYPLEFLPFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLN 672
+P+EL YL W GYPL+ +P + L++L + HS L++ W EK + LK++ L+
Sbjct: 603 LSFLPNELTYLHWHGYPLQSIPLDFDPK-NLVDLKLPHSQLEEIWDDEKDVGMLKWVDLS 662
Query: 673 HSQNLSKTPNFAKIPNLKRLELEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHIN 732
HS NL + A NL+RL LEGCTSL + +I EKLI+L+L+ C +L +LP I
Sbjct: 663 HSINLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIK 722
Query: 733 IKVLEVLILSGCSKVKKIPEFSGNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCR 792
+ L+ LILSGCS +KK P S N LL LD T I +LP SI + L +L+L NC+
Sbjct: 723 TQSLQTLILSGCSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFRRLALLNLKNCK 782
Query: 793 KLINISNAIDKMTSLQSLDLSGCSKL---GNRKRKADDVELMEPDMRGTARRRRDDDDNN 852
KL ++S+ + K+ LQ L LSGCS+L K + +E++ D + +N
Sbjct: 783 KLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSITEMPKMMHLSN 842
Query: 853 IFKKIFLWLCKAPASGIFGIPSLAGLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGN 912
I + +F +P G LT L L C+L ++P I L SL L LSGN
Sbjct: 843 IKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQSLCLSGN 902
Query: 913 NFSQLPKSISRLHNLKRLNINQCKKLVHFPELPPRILRLMSKDCISMKDF------LDIS 972
N LP+S ++L+NLK ++ CK L P LP + L + +C S++ L +
Sbjct: 903 NIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPLTPLTVG 962
Query: 973 KIDHSYFMI-EVNLLNCDQLADNKGLHR----LIISWMQKMLFR----KGTFNIMIPGSE 1032
+ HS F+ LN D A G R L+ + K +R + I P +E
Sbjct: 963 ERIHSMFIFSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEPLVGICYPATE 1022
Query: 1033 IPDWFTTTKMGSSVCVEWDPEVPNANMIRFALCVVCGLSDGNDVVNVPSFAIIASVTGKD 1092
IP WF ++G S+ + P + N + AL VV D D S + KD
Sbjct: 1023 IPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSVKCCGNFENKD 1082
Query: 1093 ----RFDTNLNDGDLMVSGFTVSGMK-KLDHIWM-----FVLPRTRTLARKISKYKEIKF 1152
RFD L + + K DH++M F++ + Y + F
Sbjct: 1083 SSFTRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLVKNVHGESNSCC-YTKASF 1142
Query: 1153 RFLLEVNYSQSVTPNVKVKKCGVVGFSLQFLSSLFVVPYIPVHCSSNCDTTSHSIESTLM 1201
F + + ++ +V KCG+ +LM
Sbjct: 1143 EFYVTDDETRKKIETCEVIKCGM----------------------------------SLM 1202
BLAST of Lsi08G001160 vs. TAIR 10
Match:
AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )
HSP 1 Score: 523.9 bits (1348), Expect = 3.8e-148
Identity = 408/1262 (32.33%), Postives = 627/1262 (49.68%), Query Frame = 0
Query: 5 SSSSSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDI 64
+S SSSSSSS++ WK DVF+SFRGED R F HL+ GI FRD+ +++ G I
Sbjct: 2 ASLPSSSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSI 61
Query: 65 SSDLLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRK 124
S +L+ AI+ SRFA+VVVS NYA+S WCL EL+KI EC K T++PIFY+VDPS VR+
Sbjct: 62 SPELIDAIKGSRFAIVVVSRNYAASSWCLDELLKIMECNKD---TIVPIFYEVDPSDVRR 121
Query: 125 QSGSFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWK 184
Q GSF + H D + V +W+ L +LA + S++W +SK+I++I I
Sbjct: 122 QRGSFGEDVESH-----SDKEKVGKWKEALKKLAAISGEDSRNWD-DSKLIKKIVKDISD 181
Query: 185 RMKPSLTVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYE 244
++ S + L+G++ ++ L S++ S D DV +GI GMGG+GKTTIA+ Y
Sbjct: 182 KL-VSTSWDDSKGLIGMSSHMDFLQSMI---SIVDKDVRMLGIWGMGGVGKTTIAKYLYN 241
Query: 245 RIRDEFEAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRK 304
++ +F+ HCF+ +V+E G+ LQ + L RMF ++ + W +I + K
Sbjct: 242 QLSGQFQVHCFMENVKEVCNRYGVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHK 301
Query: 305 KALLVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGE 364
+VLDDV+RS+Q+ L+ FG GSRII+TT + LL + G+ +++++ L E
Sbjct: 302 MVFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSH-GINLVYKVKCLPKKE 361
Query: 365 ALQLLNLSAFMER-CPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEV 424
ALQL AF E G+ E + V G PLAL++LGS L + W + +
Sbjct: 362 ALQLFCNYAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARL 421
Query: 425 EGGGNINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVE 484
+ + I L+VSYDGLDE EK IFL ++CF+ K+ + V ++L+ CG+ A+ +
Sbjct: 422 K--TYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGIT 481
Query: 485 LLIQKSLLTLSYDNKLQMHDLLQEMGRKIVRHKHV-----RDRLWCLKDIKSVVTE---- 544
+L +KSL+ S + +++HDLL++MGR++VR + V R LW +DI +++E
Sbjct: 482 ILTEKSLIVES-NGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGT 541
Query: 545 ALVQSIFLKSSKRNMV-QFPILFSRMHQLRLLNF--------NNVGLKNKLEYCIIPSEL 604
LV+ I L S+ + V F + L+LLNF V L N L Y +P +L
Sbjct: 542 QLVEGISLNLSEISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSY--LPRKL 601
Query: 605 RYLKWKGYPLEFLPFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSKT 664
RYL+W GYPL+ +P E+ L+EL M +SNL++ W + L LK + L+ + L +
Sbjct: 602 RYLRWDGYPLKTMPSRFFPEF-LVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEV 661
Query: 665 PNFAKIPNLKRLELEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVLI 724
P+ +K NL+ L L C SLV + PSI + L L CI L ++P I +K LE +
Sbjct: 662 PDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVG 721
Query: 725 LSGCSKVKKIPEFSGNTNRLLQLHLDATSISNLPSSIASLN------------------- 784
+SGCS +K PE S NT R L+L +T I LPSSI+ L+
Sbjct: 722 MSGCSSLKHFPEISWNTRR---LYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSY 781
Query: 785 -----HLTILSLNNCRKLINISNAIDKMTSLQSLDLSGCSKLGNRKRKADDVELMEPDMR 844
L L+L+ CR+L N+ + + +TSL++L++SGC + R + +E++
Sbjct: 782 LGHLVSLKSLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRISET 841
Query: 845 G-----------TARRRRDDDDN------------------------NIFKKIFLWLC-- 904
+ R D +N ++ + L +C
Sbjct: 842 SIEEIPARICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLEICQT 901
Query: 905 --------------------------------------KAPAS----------------- 964
+AP S
Sbjct: 902 MSCLRWFDLDRTSIKELPENIGNLVALEVLQASRTVIRRAPWSIARLTRLQVLAIGNSFF 961
Query: 965 ---GIFG--IPSLAGLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQLPKSIS 1024
G+ P L+ L L+L + N+ EIP I L +L+EL+LSGNNF +P SI
Sbjct: 962 TPEGLLHSLCPPLSRFDDLRALSLSNMNMTEIPNSIGNLWNLLELDLSGNNFEFIPASIK 1021
Query: 1025 RLHNLKRLNINQCKKLVHFP-ELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLN 1084
RL L RLN+N C++L P ELP +L + C S+ + IS + Y + ++ N
Sbjct: 1022 RLTRLNRLNLNNCQRLQALPDELPRGLLYIYIHSCTSL---VSISGCFNQYCLRKLVASN 1081
Query: 1085 CDQLADNKGLHRLIISWMQKMLFRKGTFNIMIPGSEIPDWFTTTKMGSSVCVEWDPEVPN 1125
C +L +++I K+ K + PGS+IP F MG S+ ++ +
Sbjct: 1082 CYKLDQ---AAQILIHRNLKLESAKPEHS-YFPGSDIPTCFNHQVMGPSLNIQLPQSESS 1141
BLAST of Lsi08G001160 vs. TAIR 10
Match:
AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 481.5 bits (1238), Expect = 2.2e-135
Identity = 343/957 (35.84%), Postives = 517/957 (54.02%), Query Frame = 0
Query: 18 KWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLAAIEASRF 77
+W YDVF+SFRG D R F HLY +L+ GI TF D+ E++ G IS +LL AIE S+
Sbjct: 11 RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 70
Query: 78 AVVVVSENYASSRWCLQELVKIFECEKRD-RMTVLPIFYKVDPSHVRKQSGSFAQAFLKH 137
+VV++++YASS WCL ELV I + K + V PIF VDPS +R Q GS+A++F KH
Sbjct: 71 LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 130
Query: 138 EIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRMKPSLTVVKED 197
+ + +K WR LT++AN+ W ++ +E++ I +IT I KR+ P +
Sbjct: 131 --KNSHPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKRL-PCQYLHVPS 190
Query: 198 QLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEFEAHCFL 257
VG+ +L +SSLL+ S D V + I+GMGGIGKTT+A+V + FE FL
Sbjct: 191 YAVGLRSRLQHISSLLSIGS---DGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFL 250
Query: 258 SDVRE-NFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFR-KKALLVLDDVE 317
+ RE + + G +LQ +LLS + + NDI +G+ K FR K+ LLV+DDV+
Sbjct: 251 ENFREYSKKPEGRTHLQHQLLSDI--LRRNDI--EFKGLDHAVKERFRSKRVLLVVDDVD 310
Query: 318 RSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLNLSAF 377
Q+ +++ FG+GSRIIITT N LL +L + + +EL E+L+L + AF
Sbjct: 311 DVHQLNSAAIDRDCFGHGSRIIITTRNMHLL-KQLRAEGSYSPKELDGDESLELFSWHAF 370
Query: 378 MERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNINEKIF 437
P + +L+H +++V G PLA+++LG+ L + + W ++ ++ N N I
Sbjct: 371 RTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDN--IQ 430
Query: 438 SCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSLLTLS 497
+ L++S++ L +K++FLD+ACFF G V IL+GC Y + LL+++ L+T+S
Sbjct: 431 AKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS 490
Query: 498 YDNKLQMHDLLQEMGRKIVRHKH-----VRDRLWCLKDIKSVVTEA----LVQSIFLKSS 557
N + MHDLL++MGR+IVR R RLW D+ V+ + ++ + LK+
Sbjct: 491 -GNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKAD 550
Query: 558 KRNMVQFPI-LFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSSSE 617
+ F + F++M +LRLL V L E+ P +LR+L W G+ LE P + S
Sbjct: 551 VMDFQYFEVEAFAKMQELRLLELRYVDLNGSYEH--FPKDLRWLCWHGFSLECFPINLSL 610
Query: 618 EYKLIELHMCHSNLKQFWQQE---KHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEG 677
E L L + +SNLK+FW+ + + +KY+ L+HS L +TP+F+ PN+++L L
Sbjct: 611 E-SLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILIN 670
Query: 678 CTSLVNIHPSI-FTAEKLIFLSLKGCINLTNLPSHI-NIKVLEVLILSGCSKVKKIPEFS 737
C SLV +H SI +KL+ L+L CI L LP I +K LE L LS CSK++++ +
Sbjct: 671 CKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDAL 730
Query: 738 GNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSG 797
G L L D T++ +PS+I L L LSLN C+ L +S+ ID + S
Sbjct: 731 GELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGL--LSDDIDNLYS-------- 790
Query: 798 CSKLGNRKRKADDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAG 857
K+ V L+ P SL+G
Sbjct: 791 --------EKSHSVSLLRP------------------------------------VSLSG 850
Query: 858 LYSLTRLNLRDCNL--EEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQC 917
L + L+L CNL E IP+ I L L +L+L GN+F LP + L NL L ++ C
Sbjct: 851 LTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNLPTDFATLPNLGELLLSDC 892
Query: 918 KKLVHFPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLH 955
KL LP +L L CI +K DISK + F +++N +C L + G+H
Sbjct: 911 SKLQSILSLPRSLLFLDVGKCIMLKRTPDISKCS-ALFKLQLN--DCISLFEIPGIH 892
BLAST of Lsi08G001160 vs. TAIR 10
Match:
AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )
HSP 1 Score: 481.5 bits (1238), Expect = 2.2e-135
Identity = 343/957 (35.84%), Postives = 517/957 (54.02%), Query Frame = 0
Query: 18 KWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSDLLAAIEASRF 77
+W YDVF+SFRG D R F HLY +L+ GI TF D+ E++ G IS +LL AIE S+
Sbjct: 14 RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 73
Query: 78 AVVVVSENYASSRWCLQELVKIFECEKRD-RMTVLPIFYKVDPSHVRKQSGSFAQAFLKH 137
+VV++++YASS WCL ELV I + K + V PIF VDPS +R Q GS+A++F KH
Sbjct: 74 LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 133
Query: 138 EIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRMKPSLTVVKED 197
+ + +K WR LT++AN+ W ++ +E++ I +IT I KR+ P +
Sbjct: 134 --KNSHPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKRL-PCQYLHVPS 193
Query: 198 QLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIRDEFEAHCFL 257
VG+ +L +SSLL+ S D V + I+GMGGIGKTT+A+V + FE FL
Sbjct: 194 YAVGLRSRLQHISSLLSIGS---DGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFL 253
Query: 258 SDVRE-NFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFR-KKALLVLDDVE 317
+ RE + + G +LQ +LLS + + NDI +G+ K FR K+ LLV+DDV+
Sbjct: 254 ENFREYSKKPEGRTHLQHQLLSDI--LRRNDI--EFKGLDHAVKERFRSKRVLLVVDDVD 313
Query: 318 RSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQLLNLSAF 377
Q+ +++ FG+GSRIIITT N LL +L + + +EL E+L+L + AF
Sbjct: 314 DVHQLNSAAIDRDCFGHGSRIIITTRNMHLL-KQLRAEGSYSPKELDGDESLELFSWHAF 373
Query: 378 MERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGGNINEKIF 437
P + +L+H +++V G PLA+++LG+ L + + W ++ ++ N N I
Sbjct: 374 RTSEPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDN--IQ 433
Query: 438 SCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQKSLLTLS 497
+ L++S++ L +K++FLD+ACFF G V IL+GC Y + LL+++ L+T+S
Sbjct: 434 AKLQISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS 493
Query: 498 YDNKLQMHDLLQEMGRKIVRHKH-----VRDRLWCLKDIKSVVTEA----LVQSIFLKSS 557
N + MHDLL++MGR+IVR R RLW D+ V+ + ++ + LK+
Sbjct: 494 -GNNIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKAD 553
Query: 558 KRNMVQFPI-LFSRMHQLRLLNFNNVGLKNKLEYCIIPSELRYLKWKGYPLEFLPFSSSE 617
+ F + F++M +LRLL V L E+ P +LR+L W G+ LE P + S
Sbjct: 554 VMDFQYFEVEAFAKMQELRLLELRYVDLNGSYEH--FPKDLRWLCWHGFSLECFPINLSL 613
Query: 618 EYKLIELHMCHSNLKQFWQQE---KHLEELKYIKLNHSQNLSKTPNFAKIPNLKRLELEG 677
E L L + +SNLK+FW+ + + +KY+ L+HS L +TP+F+ PN+++L L
Sbjct: 614 E-SLAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILIN 673
Query: 678 CTSLVNIHPSI-FTAEKLIFLSLKGCINLTNLPSHI-NIKVLEVLILSGCSKVKKIPEFS 737
C SLV +H SI +KL+ L+L CI L LP I +K LE L LS CSK++++ +
Sbjct: 674 CKSLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDAL 733
Query: 738 GNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISNAIDKMTSLQSLDLSG 797
G L L D T++ +PS+I L L LSLN C+ L +S+ ID + S
Sbjct: 734 GELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKGL--LSDDIDNLYS-------- 793
Query: 798 CSKLGNRKRKADDVELMEPDMRGTARRRRDDDDNNIFKKIFLWLCKAPASGIFGIPSLAG 857
K+ V L+ P SL+G
Sbjct: 794 --------EKSHSVSLLRP------------------------------------VSLSG 853
Query: 858 LYSLTRLNLRDCNL--EEIPQGIECLVSLVELNLSGNNFSQLPKSISRLHNLKRLNINQC 917
L + L+L CNL E IP+ I L L +L+L GN+F LP + L NL L ++ C
Sbjct: 854 LTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNLPTDFATLPNLGELLLSDC 895
Query: 918 KKLVHFPELPPRILRLMSKDCISMKDFLDISKIDHSYFMIEVNLLNCDQLADNKGLH 955
KL LP +L L CI +K DISK + F +++N +C L + G+H
Sbjct: 914 SKLQSILSLPRSLLFLDVGKCIMLKRTPDISKCS-ALFKLQLN--DCISLFEIPGIH 895
BLAST of Lsi08G001160 vs. TAIR 10
Match:
AT4G19510.1 (Disease resistance protein (TIR-NBS-LRR class) )
HSP 1 Score: 467.2 bits (1201), Expect = 4.3e-131
Identity = 343/1055 (32.51%), Postives = 537/1055 (50.90%), Query Frame = 0
Query: 8 SSSSSSSSNSKWKYDVFLSFRGEDTRGGFTDHLYKALKEKGIFTFRDEDEIEEGTDISSD 67
+SSSSS SK ++DVF+SFRG DTR FT HL K L+ KGI F D G + S
Sbjct: 44 ASSSSSIVLSKCEFDVFVSFRGADTRHDFTSHLVKYLRGKGIDVFSDAK--LRGGEYISL 103
Query: 68 LLAAIEASRFAVVVVSENYASSRWCLQELVKIFECEKRDRMTVLPIFYKVDPSHVRKQSG 127
L IE S+ ++VV SE+YA+S WCL+E+ KI + K VLPIFYKV S V Q+G
Sbjct: 104 LFDRIEQSKMSIVVFSEDYANSWWCLEEVGKIMQRRKEFNHGVLPIFYKVSKSDVSNQTG 163
Query: 128 SFAQAFLKHEIRFGEDDDNVKQWRRLLTQLANLKAWLSQSWSHESKIIEEITTTIWKRMK 187
SF F F D+ +++ + L +N++ ++ S E ++EI ++ +
Sbjct: 164 SFEAVFQSPTKIFNGDEQKIEELKVALKTASNIRGFVYPENSSEPDFLDEIVKNTFRMLN 223
Query: 188 PSLTVVKEDQLVGINFKLNELSSLLNPNSDDDDDVICVGIHGMGGIGKTTIARVCYERIR 247
V D L GI + EL LL D+D+ V VG+ GM GIGKTT+A + Y++
Sbjct: 224 ELSPCVIPDDLPGIESRSKELEKLL--MFDNDECVRVVGVLGMTGIGKTTVADIVYKQNF 283
Query: 248 DEFEAHCFLSDVRENFETSGLPYLQTKLLSRMFSFKNNDIWDVEEGIAMINKAIFRKKAL 307
F+ + FL D+ +N + GLPYL KLL ++ +N D+ + KK
Sbjct: 284 QRFDGYEFLEDIEDNSKRYGLPYLYQKLLHKLLDGENVDV----RAQGRPENFLRNKKLF 343
Query: 308 LVLDDVERSDQIMGLIPNKNSFGNGSRIIITTTNADLLSNELGVKRIFEMEELKYGEALQ 367
+VLD+V QI LI KN + GSRI+I T + LL + + + L EA++
Sbjct: 344 IVLDNVTEEKQIEYLIGKKNVYRQGSRIVIITRDKKLL--QKNADATYVVPRLNDREAME 403
Query: 368 LLNLSAFMERCPKEGYLEHCKKIVKVVGGHPLALKLLGSSLRNKGLSVWNDVIEEVEGGG 427
L L F P E +++ V G PLALKLLG L ++ W +E ++
Sbjct: 404 LFCLQVFGNHYPTEEFVDLSNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQ--V 463
Query: 428 NINEKIFSCLKVSYDGLDEWEKEIFLDVACFFKGKRREVVEEILNGCGFYAKTRVELLIQ 487
N ++++ LK SY LD+ +K +FLD+ACFF
Sbjct: 464 NPDKELQKELKSSYKALDDDQKSVFLDIACFF---------------------------- 523
Query: 488 KSLLTLSYDNKLQMHDLLQEMGRKIVRHKHVRD-----RLWCLKDIKSVVTE----ALVQ 547
+++MHDLL MG++I + K +R RLW KDI+ ++ V+
Sbjct: 524 ----------RIEMHDLLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTECVR 583
Query: 548 SIFLKSSK-RNMVQFPILFSRMHQLRLLNFNNVGLKNKLEYC-------------IIPSE 607
IFL S+ R + FP F+ + +L+ L F++ + ++C P E
Sbjct: 584 GIFLNMSEVRRIKLFPAAFTMLSKLKFLKFHS---SHCSQWCDNDHIFQCSKVPDHFPDE 643
Query: 608 LRYLKWKGYPLEFLPFSSSEEYKLIELHMCHSNLKQFWQQEKHLEELKYIKLNHSQNLSK 667
L YL W+GYP + LP S + +L++L + +S++KQ W+ EK+ E L+++ L S++L
Sbjct: 644 LVYLHWQGYPYDCLP-SDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLN 703
Query: 668 TPNFAKIPNLKRLELEGCTSLVNIHPSIFTAEKLIFLSLKGCINLTNLPSHINIKVLEVL 727
++ NL+RL+LEGCTSL ++ S+ +LI+L+L+ C +L +LP IK L+ L
Sbjct: 704 LSGLSRAKNLERLDLEGCTSL-DLLGSVKQMNELIYLNLRDCTSLESLPKGFKIKSLKTL 763
Query: 728 ILSGCSKVKKIPEFSGNTNRLLQLHLDATSISNLPSSIASLNHLTILSLNNCRKLINISN 787
ILSGC K+K +F + + LHL+ T+I + I SL+ L +L+L NC KL + N
Sbjct: 764 ILSGCLKLK---DFHIISESIESLHLEGTAIERVVEHIESLHSLILLNLKNCEKLKYLPN 823
Query: 788 AIDKMTSLQSLDLSGCSKLGNR---KRKADDVELMEPDMRGTARRRRDDDD--NNIFKKI 847
+ K+ SLQ L LSGCS L + K K + +E++ D GT+ ++ + +N+
Sbjct: 824 DLYKLKSLQELVLSGCSALESLPPIKEKMECLEILLMD--GTSIKQTPEMSCLSNLKICS 883
Query: 848 FLWLCKAPASGIFGIPSLAGLYSLTRLNLRDCNLEEIPQGIECLVSLVELNLSGNNFSQL 907
F ++G+ +P +G L+ L L +CN++++P L SL L LS NN L
Sbjct: 884 FCRPVIDDSTGLVVLP-FSGNSFLSDLYLTNCNIDKLPDKFSSLRSLRCLCLSRNNIETL 943
Query: 908 PKSISRLHNLKRLNINQCKKLVHFPELPPRILRLMSKDCISMKDFLD------ISKIDHS 967
P+SI +L++L L++ C +L P LP + L + C S+++ +++ H+
Sbjct: 944 PESIEKLYSLLLLDLKHCCRLKSLPLLPSNLQYLDAHGCGSLENVSKPLTIPLVTERMHT 1003
Query: 968 YFM----IEVNLLNCDQLADNKGLHRLIISWMQKMLFRKGTF-----NIMIPGSEIPDWF 1020
F+ ++N + + L +++ + KG + PG +IP WF
Sbjct: 1004 TFIFTDCFKLNQAEKEDIVAQAQLKSQLLARTSRHHNHKGLLLDPLVAVCFPGHDIPSWF 1037
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q40392 | 1.5e-157 | 36.29 | TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1 | [more] |
V9M398 | 7.5e-157 | 40.10 | Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... | [more] |
V9M2S5 | 3.9e-153 | 39.25 | Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... | [more] |
Q9SZ66 | 6.6e-153 | 33.15 | Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=... | [more] |
A0A290U7C4 | 8.4e-148 | 36.93 | Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3CBN9 | 0.0e+00 | 85.34 | TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498654 PE=4 SV=1 | [more] |
A0A6J1I8R5 | 0.0e+00 | 77.13 | TMV resistance protein N-like OS=Cucurbita maxima OX=3661 GN=LOC111472025 PE=4 S... | [more] |
A0A6J1EBI7 | 0.0e+00 | 76.86 | TMV resistance protein N-like OS=Cucurbita moschata OX=3662 GN=LOC111432675 PE=4... | [more] |
A0A6J1CKI7 | 0.0e+00 | 76.48 | TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
A0A6J1CL32 | 0.0e+00 | 74.24 | TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11... | [more] |
Match Name | E-value | Identity | Description | |
AT4G12010.1 | 4.7e-154 | 33.15 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT5G17680.1 | 3.8e-148 | 32.33 | disease resistance protein (TIR-NBS-LRR class), putative | [more] |
AT5G36930.1 | 2.2e-135 | 35.84 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT5G36930.2 | 2.2e-135 | 35.84 | Disease resistance protein (TIR-NBS-LRR class) family | [more] |
AT4G19510.1 | 4.3e-131 | 32.51 | Disease resistance protein (TIR-NBS-LRR class) | [more] |