Lsi08G001100 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi08G001100 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 2.4e-45
Identity = 267/1050 (25.43%), Postives = 384/1050 (36.57%), Query Frame = 0

Query: 5   SSSSFPS----MKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENI 64
           SSSS PS    + YDVF+SFRG      FT  L   L   G+ TF  +D+ + G   E I
Sbjct: 14  SSSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTF-RDDKLRRG---EAI 73

Query: 65  VQEAI--ILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGE--DYEEVVIPVLYQHHHPS 124
             E +  I  SR  V+V +++Y  S  CL EL KIME   +  D    V P+ Y H  PS
Sbjct: 74  APELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFY-HVDPS 133

Query: 125 CLVKELQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQ 184
                            VR +EG F ++  G+                            
Sbjct: 134 ----------------HVRKQEGSFGEAFAGY---------------------------- 193

Query: 185 HDPNLFSDQDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEII----- 244
                       N+  ++PRW++AL    NL           +ES+ IKE+ + I     
Sbjct: 194 ----------GENLKDKIPRWRTALTEAANLSGWPLQD---GYESNQIKEITDSIFRRLK 253

Query: 245 -NGVKESSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFC 304
              +   + LVGI   + +M   L + S+D+  +G+ G+GGIGKTTIAK +Y  L  +F 
Sbjct: 254 CKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKVIYNELSREFE 313

Query: 305 H----NHCFLRVSGQKLVSMQNQLLLR-LCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVL 364
           +     +   + + Q +  +QNQLL   L G+  + I +  HGA +IK  L +K V IVL
Sbjct: 314 YMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDILSSKIVFIVL 373

Query: 365 DGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSL 424
           D VD++ QLE L+   +W   GSR+I+TTRN+++    K       Y+++ L+ + A  L
Sbjct: 374 DDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQKVDDL---YEVKGLNFEEACEL 433

Query: 425 FCNHAFGE-----------------------------------------------DHEPP 484
           F  +AF +                                               D EP 
Sbjct: 434 FSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESELRKLDREPE 493

Query: 485 DE---NFRSSYNKL--------------------------------------HLLAERNL 544
            E     + SY+ L                                        L ++ L
Sbjct: 494 AEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHAEIGIKNLNDKCL 553

Query: 545 IQISNENVHMHTLVRCMGRGIARE----ERETQSRIW----------LRKDIRRV----- 604
           I +    + MH L++ MG  I RE    E    SR+W            K I+RV     
Sbjct: 554 ITLQYNRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAYKGIKRVETISL 613

Query: 605 ---------------------------------FHQE----------------------- 664
                                            F  E                       
Sbjct: 614 DLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYDVVMKNASKMRL 673

Query: 665 --------------------------------------------------KEFSWLKEID 724
                                                             K+   LK ID
Sbjct: 674 GRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQLRLGNKDLEMLKVID 733

Query: 725 VSGSENLIETPDFSKVPNLQRLILRNCERLRMVHPSISTLNHLVSLDMAGLK-------- 757
           +S S  L +  +FS +PNL+RL LR C  L  +HPS+  +  L +L +   K        
Sbjct: 734 LSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLKSCKKLKNLPDS 793

BLAST of Lsi08G001100 vs. ExPASy Swiss-Prot
Match: F4J339 (Probable disease resistance protein RPP1 OS=Arabidopsis thaliana OX=3702 GN=RPP1 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 5.0e-43
Identity = 228/983 (23.19%), Postives = 383/983 (38.96%), Query Frame = 0

Query: 4   PSSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIVQE 63
           P++S   + K+DVF SF G      F   + E     G+ TF+ N+     + S++I  E
Sbjct: 88  PATSVSRNWKHDVFPSFHGADVRRTFLSHIMESFRRKGIDTFIDNNI----ERSKSIGPE 147

Query: 64  --AIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKE 123
               I  S+  +V+L++ Y +S  CL EL++IM+ R +   ++V+ + Y+   P+ + K+
Sbjct: 148 LKEAIKGSKIAIVLLSRKYASSSWCLDELAEIMKCR-QMVGQIVMTIFYE-VDPTDIKKQ 207

Query: 124 LQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNL 183
             +FGK                             F K+   K +E              
Sbjct: 208 TGEFGK----------------------------AFTKTCRGKPKE-------------- 267

Query: 184 FSDQDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGV------- 243
                      +V RW+ ALE +  +    + +    +E+D+I+++   ++ +       
Sbjct: 268 -----------QVERWRKALEDVATIAG--YHSHSWRNEADMIEKISTDVSNMLNSFTPS 327

Query: 244 KESSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKF----- 303
           ++   LVG+   +  ++ LL L  +++  IGI G  GIGKTTIA+ L+  +  +F     
Sbjct: 328 RDFDGLVGMRAHMDMLEQLLRLDLDEVRMIGIWGPPGIGKTTIARFLFNQVSDRFQLSAI 387

Query: 304 ------CH-NHCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVL 363
                 C+   CF   S Q  + +QNQ+L ++    + +I +      + ++ LR+KKV 
Sbjct: 388 MVNIKGCYPRPCFDEYSAQ--LQLQNQMLSQMINHKDIMISH----LGVAQERLRDKKVF 447

Query: 364 IVLDGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSA 423
           +VLD VD+  QL+ L     WF  GSRII+TT +  + +     H    Y +E+  +D A
Sbjct: 448 LVLDEVDQLGQLDALAKETRWFGPGSRIIITTEDLGVLKAHGINH---VYKVEYPSNDEA 507

Query: 424 FSLFCNHAFG--EDHEPPDE---------------------------------------- 483
           F +FC +AFG  + HE  DE                                        
Sbjct: 508 FQIFCMNAFGQKQPHEGFDEIAWEVTCLAGELPLGLKVLGSALRGKSKREWERTLPRLKT 567

Query: 484 ---------------------------------------------NFRSSYNKLHLLAER 543
                                                         F      LHLLA++
Sbjct: 568 SLDGKIGSIIQFSYDVLCDEDKYLFLYIACLFNGESTTKVKELLGKFLDVKQGLHLLAQK 627

Query: 544 NLIQISNENVHMHTLVRCMGRGIAR------------------------EERETQSRIWL 603
           +LI    E +HMHTL+   GR  +R                        ++  T SR ++
Sbjct: 628 SLISFDGERIHMHTLLEQFGRETSRKQFVHHGFTKRQLLVGARGICEVLDDDTTDSRRFI 687

Query: 604 ------------------------------------------------------------ 663
                                                                       
Sbjct: 688 GIHLELSNTEEELNISEKVLERVHDFHFVRIDASFQPERLQLALQDLIYHSPKIRSLNWY 747

Query: 664 ----------------------RKDIRRVFHQEKEFSWLKEIDVSGSENLIETPDFSKVP 723
                                   ++R+++   K+   LK +D+S S  L E P+ S   
Sbjct: 748 GYESLCLPSTFNPEFLVELDMRSSNLRKLWEGTKQLRNLKWMDLSYSSYLKELPNLSTAT 807

Query: 724 NLQRLILRNCERLRMVHPSISTLNHLVSLDM---AGLKSFPEIEKHTKSNLTELHLDGTL 761
           NL+ L LRNC  L  +  SI  L  L  LD+   + L+  P IE  TK    +L    +L
Sbjct: 808 NLEELKLRNCSSLVELPSSIEKLTSLQILDLENCSSLEKLPAIENATKLRELKLQNCSSL 867

BLAST of Lsi08G001100 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.1e-42
Identity = 210/848 (24.76%), Postives = 346/848 (40.80%), Query Frame = 0

Query: 5   SSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIVQEA 64
           +SSS     YDVF+SFRG      F   L   L   G+ TFM +   K G    + + +A
Sbjct: 3   TSSSHHGRSYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKA 62

Query: 65  IILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKELQD 124
            I  SR+ VVV +K+Y +S  CL EL KI+E   E +E +V+PV Y              
Sbjct: 63  -IGESRFAVVVFSKNYASSTWCLEELVKILEIH-EKFELIVVPVFYD------------- 122

Query: 125 FGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNLFSD 184
               ++   VR + G +                              +   + + NL  D
Sbjct: 123 ----VDPSTVRKQNGEY-----------------------------AVCFTKFEANLVDD 182

Query: 185 QDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEV-KEIINGVKES-----S 244
           +D      +V RW+ AL  + N+            ES  I+++ K+I +    S      
Sbjct: 183 RD------KVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNR 242

Query: 245 KLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNHCFLR- 304
            LVGI  Q+ ++ +LL +    +  +GI GMGG+GKTT A+AL+      F  + CFL  
Sbjct: 243 DLVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNF-ESACFLED 302

Query: 305 ----VSGQKLVSMQNQLLLRLCGKLEEV-IKNEDHGAQLIKKCLRNKKVLIVLDGVDEKR 364
               +    L+ +Q  LL +L  K+E V   + +    ++K+ L +KKVL+VLD V+   
Sbjct: 303 VKEYLQHHTLLYLQKTLLSKLL-KVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHND 362

Query: 365 QLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFE-YDIEFLDHDSAFSLFCNHAF 424
           QL+KLVG+ DWF SGSRI++TTR+  L +     H + E Y+I+ L+ D A  LF  HAF
Sbjct: 363 QLDKLVGAEDWFGSGSRIVITTRDMKLLK----NHDVHETYEIKVLEKDEAIELFNLHAF 422

Query: 425 GEDHEPPDENFRS----------------------------------------------- 484
                 P++ F+                                                
Sbjct: 423 --KRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLKDNPEGEIM 482

Query: 485 --------------------------SYNK-----------------LHLLAERNLIQIS 544
                                      YN+                 +  L E++LI I 
Sbjct: 483 ATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFIL 542

Query: 545 NENVHMHTLVRCMGRGIAREERETQSRIWLRKDIRRVFHQEKEFSWLKEIDVSGSENLIE 604
            + + MH L++ MGR IA +E     RI+  +D++     +     ++ + ++  E   E
Sbjct: 543 EDKIQMHDLMQEMGRQIAVQE-SPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEE 602

Query: 605 -------TPDFSKVPNLQRLILRNCERLRMVHPSISTLNHLVSLDMAGLKSFPEIEKHTK 664
                  + +  K     R++++         P     N L+ L+     S         
Sbjct: 603 GELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEP 662

Query: 665 SNLTELHLDGTLIKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNLNGCKS 724
           S L  L + G+ I +L      L  L  L+L  C KL   P   R + +L+ L L+ C +
Sbjct: 663 SKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTP-DFRMITNLERLILSSCDA 722

Query: 725 LHQIPFTLGAIQSLTMLDIGGTLIDQAPHAITSLRNLESLNCERLSRNIWWSLTCDLVGN 742
           L ++  ++G +++L +L++   +  +   AI     LE L+         +     +   
Sbjct: 723 LVEVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLN-------YCFNLKMFPE 774

BLAST of Lsi08G001100 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.1e-41
Identity = 136/433 (31.41%), Postives = 215/433 (49.65%), Query Frame = 0

Query: 5   SSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIVQEA 64
           SSSS     YDVF+SFRG      FT  L E L++ G+ TF  + R ++G      + +A
Sbjct: 3   SSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKA 62

Query: 65  IILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKELQD 124
            I  S++ +VV +++Y TS  CL EL KIME +   +++ VIP+ Y    PS +  + + 
Sbjct: 63  -IEESQFAIVVFSENYATSRWCLNELVKIMECKTR-FKQTVIPIFYD-VDPSHVRNQKES 122

Query: 125 FGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNLFSD 184
           F K  E+++ + K+                              D+E             
Sbjct: 123 FAKAFEEHETKYKD------------------------------DVE------------- 182

Query: 185 QDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIIN------GVKESS 244
                    + RW+ AL    NL  +    +    ++D I+++ + I+       +    
Sbjct: 183 --------GIQRWRIALNEAANLKGSCDNRD--KTDADCIRQIVDQISSKLCKISLSYLQ 242

Query: 245 KLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNH----- 304
            +VGI   L ++++LL++G N +  +GI GMGG+GKTTIA+A++  L  +   ++     
Sbjct: 243 NIVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGA 302

Query: 305 CFL---RVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDE 364
           CFL   + + + + S+QN LL  L  + +    NE+ G   +   LR+KKVLIVLD +D 
Sbjct: 303 CFLKDIKENKRGMHSLQNALLSELL-REKANYNNEEDGKHQMASRLRSKKVLIVLDDIDN 362

Query: 365 K-RQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNH 423
           K   LE L G  DWF +GSRII+TTR+++L      K+ I  Y++  L    +  LF  H
Sbjct: 363 KDHYLEYLAGDLDWFGNGSRIIITTRDKHLIE----KNDII-YEVTALPDHESIQLFKQH 371

BLAST of Lsi08G001100 vs. ExPASy Swiss-Prot
Match: Q9FI14 (Disease resistance protein TAO1 OS=Arabidopsis thaliana OX=3702 GN=TAO1 PE=3 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 8.0e-41
Identity = 224/913 (24.53%), Postives = 378/913 (41.40%), Query Frame = 0

Query: 2   NPPSSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIV 61
           +PPSS S  +  + VF+SFRG          + +E   NG+T F+ N+  + G     ++
Sbjct: 29  SPPSSLS-QNWLHPVFLSFRGEDVRKGLLSHIQKEFQRNGITPFIDNEMKRGGSIGPELL 88

Query: 62  QEAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKE 121
           Q   I  S+  +++L+++Y +S  CL EL +IM+ R E+  + V+ V Y    PS + K+
Sbjct: 89  Q--AIRGSKIAIILLSRNYGSSKWCLDELVEIMKCR-EELGQTVMTVFYD-VDPSDVRKQ 148

Query: 122 LQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNL 181
             DFGK              FK  C     ++V ++ ++ ++                  
Sbjct: 149 KGDFGK-------------VFKKTCVGRPEEMVQRWKQALTSA----------------- 208

Query: 182 FSDQDNSNVLMEVPR-WKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGV--KESS 241
                 +N+L E  R W++  + I                  I K+V ++++    K+  
Sbjct: 209 ------ANILGEDSRNWENEADMIIK----------------ISKDVSDVLSFTPSKDFD 268

Query: 242 KLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNL------------- 301
           + VGI     ++ +LL L   ++  IGI G  GIGKTTI++ LY  L             
Sbjct: 269 EFVGIEAHTTEITSLLQLDLEEVRMIGIWGPAGIGKTTISRVLYNKLFHQFQLGAIIDNI 328

Query: 302 ---QPKFCHNHCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVL 361
               P+ CH+    +      + +Q +LL ++  + + V+ +      + ++ L++KKVL
Sbjct: 329 KVRYPRPCHDEYSAK------LQLQKELLSQMINQKDMVVPH----LGVAQERLKDKKVL 388

Query: 362 IVLDGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSA 421
           +VLD VD   QL+ +     WF  GSRIIV T++  L +    K   + Y ++F   D A
Sbjct: 389 LVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQDLKLLKAHGIK---YIYKVDFPTSDEA 448

Query: 422 FSLFCNHAFGED----------------------------------------------HE 481
             +FC +AFGE                                                 
Sbjct: 449 LEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLRVMGSYLRRMSKQEWAKSIPRLRT 508

Query: 482 PPDENFRS----SYNK--------------------------------------LHLLAE 541
             D++  S    SYN                                       L +LA+
Sbjct: 509 SLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERIETLEVFLAKKSVDMRQGLQILAD 568

Query: 542 RNLIQISNENVHMHTLVRCMGRGIAREE--RETQSRIWL--RKDIRRVFHQEKEFSWLKE 601
           ++L+ ++  N+ MH L+  +G  I R++   +   R +L   +DI  V   +     L  
Sbjct: 569 KSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKPGKRQFLVDTEDICEVLTDDTGTRTLIG 628

Query: 602 ID------VSGSENLIETPDFSKVPNLQRLILRN-------------------CERLRMV 661
           ID      + G  N+ E   F ++ NLQ L   +                     +LR++
Sbjct: 629 IDLELSGVIEGVINISERA-FERMCNLQFLRFHHPYGDRCHDILYLPQGLSHISRKLRLL 688

Query: 662 H---------PSISTLNHLVSLDM--------------------------AGLKSFPEIE 721
           H         P       LV ++M                            LK  P+  
Sbjct: 689 HWERYPLTCLPPKFNPEFLVKINMRDSMLEKLWDGNEPIRNLKWMDLSFCVNLKELPDFS 748

Query: 722 KHTKSNLTELHLDGTL-IKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNL 742
             T +NL EL L   L + +LP S+G  T+L  L+L DC+ L  LP SI  L +L+ L L
Sbjct: 749 --TATNLQELRLINCLSLVELPSSIGNATNLLELDLIDCSSLVKLPSSIGNLTNLKKLFL 808

BLAST of Lsi08G001100 vs. ExPASy TrEMBL
Match: A0A5A7T8K2 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G00970 PE=4 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 1.3e-304
Identity = 594/935 (63.53%), Postives = 686/935 (73.37%), Query Frame = 0

Query: 234  VKESSKLVGISPQLHQ-MDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHN 293
            VK   KL+GISPQLHQ MDNL++LGSND+ FIGIVGM GIGKTT+AKA+ + L+PKF +N
Sbjct: 4    VKGPPKLIGISPQLHQIMDNLVELGSNDVGFIGIVGMSGIGKTTLAKAVCQELEPKFGYN 63

Query: 294  -HCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEK 353
             HCFLRV  +KLV++Q +LLLR  GKL+ VIKNEDHGA+LIKKCLRNKKVLIVLDGVDE 
Sbjct: 64   SHCFLRVHTEKLVTVQKKLLLRFGGKLDIVIKNEDHGAKLIKKCLRNKKVLIVLDGVDEI 123

Query: 354  RQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAF 413
            RQ++KL GSPDWFS GSRII+TTRNRNLCRQ KYKHKIFEYD+E LDH+SAF LFCNHAF
Sbjct: 124  RQMKKLAGSPDWFSPGSRIIITTRNRNLCRQQKYKHKIFEYDVECLDHNSAFLLFCNHAF 183

Query: 414  GED-HEPPDENFRSSYNKLHLLAERNLIQISNE-----------------------NVHM 473
            G+D HEPP+E  R    ++ L   + ++ +  E                       NV +
Sbjct: 184  GKDVHEPPNETLRYLTEEMGLKYVKGIVIMDMEDKQLLLDATPFAYMTELKILKMNNVKV 243

Query: 474  HTLVRCMGRGI---------------AREERETQSRIWLRKDIRRVFHQEKEFSWLKEID 533
            +  +R +   +                 E R           + +++  +KEF WLKEID
Sbjct: 244  YGDIRFLSNELRFLYWNGYPDYCLPFTFEPRHILELHLRNSQLDQLWEGQKEFIWLKEID 303

Query: 534  VSGSENLIETPDFSKVPNLQRLILRNCERLRMVHPSISTLNHLVSLDMA----------- 593
            VSGSE L+ETPDFSKVPNLQRLILRNCERL  VHPSI+TLNHL+ +DMA           
Sbjct: 304  VSGSEYLVETPDFSKVPNLQRLILRNCERLCEVHPSITTLNHLLLVDMANCVNLKTFPPK 363

Query: 594  ---------------GLKSFPEIEKHTKSNLTELHLDGTLIKDLPFSLGLLTHLALLNLR 653
                           G++SFPEIE+ TKS LTELHLDG LI D                 
Sbjct: 364  LITSKRLQTLILSNSGIESFPEIEEPTKS-LTELHLDGMLIHD----------------- 423

Query: 654  DCTKLSSLPRSIRKLISLQTLNLNGCKSLHQIPFTLGAIQSLTMLDIGGTLIDQAPHAIT 713
                   LP SI +L+SLQTLNLNGCKSLHQIP TL  I SLTMLDIGGTLID AP+AIT
Sbjct: 424  -------LPESICRLMSLQTLNLNGCKSLHQIPSTLETISSLTMLDIGGTLIDHAPNAIT 483

Query: 714  SLRNLESLNCERLSRNIWWSLTCDLVGNNNNGVLPLRDLNLSDCNLEDEDIPDDMKCLSL 773
            S  NLE+LNCE+LSRNIW SL+  LVG  NNGVLPLRDLNLS C L+DEDIPDD+ CLS 
Sbjct: 484  SFSNLETLNCEKLSRNIWKSLS-GLVG-INNGVLPLRDLNLSACKLDDEDIPDDLNCLSS 543

Query: 774  LEILDLSKNSFVRLKESLTQLINLKALCLNDCNNIQPQLLPKLPASLRYMGGQNNSQHHI 833
            LEILDLS NSFV+LKESLTQL NLKAL LNDCN+IQPQLLPK   +L+Y+GG  NS   I
Sbjct: 544  LEILDLSNNSFVKLKESLTQLTNLKALYLNDCNDIQPQLLPKFLTTLQYVGGL-NSLKSI 603

Query: 834  SQNHEGSYGRNSASSTSNSPRIVPEIENVYDDEKEEYSTEEVDNINYGNKMGKHISDQLS 893
            SQN EGS G  SA    + P+ V EIEN+  + +EE S EEVDNI    KMGK IS QL+
Sbjct: 604  SQNDEGSSGSKSACPPFSPPKTVTEIENIVYEIEEEKSHEEVDNIKV-KKMGKQIS-QLN 663

Query: 894  SDSKLEVFCKDLVIGMEEQLEEVHKLLDLKESNEVQFVWICGMAGIGKTTIAQVVYNEIL 953
            + SK    C   ++GM+EQLEEV KLLDLKES EVQFVWICGM GIGKTTIA+VVYNEI+
Sbjct: 664  THSKFGKVCGKELVGMKEQLEEVLKLLDLKESTEVQFVWICGMDGIGKTTIAEVVYNEIV 723

Query: 954  HKFHTSCFLHISQKNNLVSLQHQLLSQLLLKDDIEVRDEDYGANMIENQMKSRMVLIVLD 1013
             KF +SCFLH++Q+ NLVSLQHQLLS+LLLKDDIE+RDED GA MIEN+MKSRM L+VLD
Sbjct: 724  GKFESSCFLHVNQRKNLVSLQHQLLSRLLLKDDIEIRDEDRGAQMIENEMKSRMALVVLD 783

Query: 1014 DIDEKRQVEVLVGSPDWFGQGSRVIITTRNRDILHQLNYKDKIREYNVKLLSQESANLLF 1073
            DIDEKRQVEVLVGSPDWFG GSR+IITTRNR+I+HQLN+KDK+REYNVKLLSQE+ANLLF
Sbjct: 784  DIDEKRQVEVLVGSPDWFGHGSRIIITTRNREIIHQLNHKDKMREYNVKLLSQENANLLF 843

Query: 1074 CKYAFGSYYDGQDKNDDVLCSEIVKRVGRLPLALILIGSALHNKDEDIWKETLRILCNVE 1102
            CKYAF +  + Q  N D LC+EIVK+VGRLPLALILIGSAL NKD   WKE LRILCNVE
Sbjct: 844  CKYAFENNNNAQHTN-DALCTEIVKKVGRLPLALILIGSALRNKDAAKWKEMLRILCNVE 903

BLAST of Lsi08G001100 vs. ExPASy TrEMBL
Match: A0A1S3CPB4 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103503300 PE=4 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 3.8e-203
Identity = 445/850 (52.35%), Postives = 503/850 (59.18%), Query Frame = 0

Query: 1   MNPPS---SSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDS 60
           MNPPS   +SS P     VFISF     D KF  +LA+E  N  L TFMS+     GDD 
Sbjct: 1   MNPPSTSPTSSSPPNTCQVFISFATA--DSKFVSELAKEFRNIRLRTFMSS-----GDDG 60

Query: 61  ENIVQEAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSC 120
           E I+ E  +  SR+ VVVLTKDY+ SI+CLRELS+IMEY+ E YEE VIPV YQ   PSC
Sbjct: 61  E-IIHEDAMKKSRHFVVVLTKDYVNSIKCLRELSRIMEYKDEGYEE-VIPVFYQ--DPSC 120

Query: 121 LVKELQDFGKRLEDY-KVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQ 180
           LVKELQDFGKRLEDY ++  +EG    ++C FLKT L  + NKSFS K E          
Sbjct: 121 LVKELQDFGKRLEDYEEIIKEEGLIEFTHCWFLKTDLAKRLNKSFSEKEE---------- 180

Query: 181 HDPNLFSDQDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGVKE 240
                    + SN+LMEVPRWK ALE I NLP +IF+ E  S ESD+IKEV EII  VK 
Sbjct: 181 ---------NKSNLLMEVPRWKFALERINNLPKSIFVGE-ESLESDVIKEVTEIIYKVKA 240

Query: 241 SSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNHCFL 300
           S KLVGISP LHQMDNLL+LGSNDI FIGIVG+GGIGKTTIAK LY+ L+ KF +  CFL
Sbjct: 241 SPKLVGISPPLHQMDNLLNLGSNDIRFIGIVGLGGIGKTTIAKVLYEKLEHKFRY-CCFL 300

Query: 301 RVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLEK 360
           R   QKLVS+Q +LLLRLCGK + VIKNEDHGA+LIKKCLRNKKVLIVLDGVDE+RQ+EK
Sbjct: 301 RDFDQKLVSIQKKLLLRLCGKFDIVIKNEDHGAKLIKKCLRNKKVLIVLDGVDERRQIEK 360

Query: 361 LVG-SPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAFGEDH 420
           LVG S DWFS GSRII+TTRNRNLC QPKYKHK+ EY++EFLDH +AFSLFCNHAFGE+H
Sbjct: 361 LVGSSQDWFSPGSRIIITTRNRNLCCQPKYKHKMLEYNVEFLDHHNAFSLFCNHAFGENH 420

Query: 421 EPPDENF----------------------------------------------------- 480
           EP DENF                                                     
Sbjct: 421 EPLDENFRYLAEEMVKMVEGHPLALIKIGSHLHGKDIDKWEESLEKVHKLLYKYLFCEVF 480

Query: 481 ----------------------------------------RSSYNKLHLLAERNLIQISN 540
                                                   RS YNKL+LL +RNLIQ+S+
Sbjct: 481 RTSYEELDDESQQVFLDLACFFNNGMSMDRAIEILESFGYRSPYNKLNLLTQRNLIQVSH 540

Query: 541 ENVHMHTLVRCMGRGIAREERETQSRIWLRKDIRRVFHQE-------------------- 600
             V MHTLV CMGRGI + ERETQSRIWLRK IR +F +E                    
Sbjct: 541 GMVQMHTLVHCMGRGIVQGERETQSRIWLRKHIRHMFGKEKGLEDVEGIVVMDMEEEELV 600

Query: 601 ------------------------------------------------------------ 635
                                                                       
Sbjct: 601 LDAKSFAYMNKLKLLEINNVRVDEDIQFLSNKLGILRWNGYPSKYLPSTFQPQSLLELHL 660

BLAST of Lsi08G001100 vs. ExPASy TrEMBL
Match: A0A6J1CJX6 (LOW QUALITY PROTEIN: TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111012105 PE=4 SV=1)

HSP 1 Score: 701.0 bits (1808), Expect = 8.2e-198
Identity = 528/1462 (36.11%), Postives = 709/1462 (48.50%), Query Frame = 0

Query: 5    SSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFK--FGDDSENIVQ 64
            S S  P M +DVF+SF    + + FT  L   L +         D+ K  F D+    + 
Sbjct: 4    SLSHPPQMSFDVFLSF--SKEYELFTANLCRSLRSRLRIRIFMEDKTKLSFEDNLSKKIV 63

Query: 65   EAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKEL 124
            EA I  S   VVVL+K Y +S  CL+EL+KI+++  E+   +V+PV              
Sbjct: 64   EA-IEGSMCSVVVLSKHYASSKSCLKELAKIIDHM-ENTTHLVLPVF------------- 123

Query: 125  QDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNLF 184
                K ++   VR + G   KS            F++   + +E              L 
Sbjct: 124  ----KDVDPIHVRHQSGNLKKS------------FDEYEKSAQE--------------LM 183

Query: 185  SDQDNSNVLMEVPRWKSALEGITNL--PTTIFLTEIRSHESDIIKEVKEIINGVK---ES 244
             ++ N   L E+ RW+ AL  I NL  P T  ++ +    S I K++ ++++ +K   E+
Sbjct: 184  DNEQNEKHLKEIERWRDALTKIANLSGPATKLVSGVIG-ISRITKQISDMLHHLKLVAEN 243

Query: 245  SKLVGISPQLHQMDNLL-DLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNHCFL 304
              LV + P+L +M+ LL +L SND+ F+ IVGMGGIGKTTIA+  Y  L PKF  + CFL
Sbjct: 244  RYLVEMPPRLGRMNMLLGNLESNDVRFVAIVGMGGIGKTTIAELFYDQLAPKFMKS-CFL 303

Query: 305  RVSGQKLVSMQNQLLLR-LCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLE 364
            R++G  LVS+Q QLL + L  K    I NE+ G + I++ L+ ++VLIVLDG+ EK QL+
Sbjct: 304  RIAGCNLVSLQQQLLSQLLLSKTNIHILNENDGVRKIQRHLKFERVLIVLDGIHEKMQLK 363

Query: 365  KLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAFGEDH 424
             L G+P WF   SR+I+TTRN+++   P Y+ K+ EY++E L   +A SL C HAFG+D 
Sbjct: 364  MLAGNPYWFGRKSRVIITTRNKDIFHHPNYRDKVQEYNVELLHDTTAHSLLCKHAFGDD- 423

Query: 425  EPPDENFR---------------------------------------------------- 484
            EPP+E F+                                                    
Sbjct: 424  EPPNETFKDLCNEIIEKVGRLPLALIKIGSSLYGQTMDIWEGALKSYHKLVYENLFSAIL 483

Query: 485  ----------------------------------------SSYNKLHLLAERNLIQISNE 544
                                                    S + KL LL +R LI +S+E
Sbjct: 484  KTSYEGLEVESQQIFLDCACFFNGEKVDRVVEILESFGYSSPHTKLQLLVDRCLIDVSDE 543

Query: 545  NVHMHTLVRCMGRGIA-REERETQSRIWLRKDIRRVFHQE-------------------- 604
             + MH L+ CMGRGI    ++  QSRIWLR+D  RVFH++                    
Sbjct: 544  RIQMHNLILCMGRGIVLATQQGKQSRIWLREDAHRVFHEKSELKYIQGIVMDLEEGEELV 603

Query: 605  ------------------------------------------------------------ 664
                                                                        
Sbjct: 604  VEAKSFTDMTELKILQINNVELSEDIEFLSNQLRLLHWPGYPSKCLPSTFQPPSLLELHL 663

Query: 665  ------------KEFSWLKEIDVSGSENLIETPDFSKVPNLQRLILRNCERLRMVHPSIS 724
                        K+F  LKEIDVS S++L+ETPDFS+VPNL+RLILRNC +L +VHPSI+
Sbjct: 664  HGSKLERLWEGRKKFEKLKEIDVSESKHLVETPDFSEVPNLRRLILRNCGKLDVVHPSIN 723

Query: 725  TLNHLVSLDM--------------------------AGLKSFPEIEKHTKSNLTELHLDG 784
            +L  LV LD+                          +GL+ FPE+ +     LTELHL+G
Sbjct: 724  SLERLVLLDLEGCISLRSFSSNYINFKSLKTLMLSNSGLECFPEVGE-PMDRLTELHLEG 783

Query: 785  TLIKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNLNGCKSLHQIPFTLGA 844
            T+I +LP S+  LT L LLNLR+CT LSSLP  I  L +L+TL LNGCK L +IP  LG 
Sbjct: 784  TIINELPLSIRHLTSLVLLNLRNCTGLSSLPSEIGSLSNLKTLILNGCKELKEIPPNLGD 843

Query: 845  IQSLTMLDIGGTLIDQAPHAITSLRNLESLNCERLS---------RNIWWSLTC------ 904
            ++SL  LDIG T I QAPHAI SLRNL  LNCE L+            W +L        
Sbjct: 844  VESLEELDIGRTSISQAPHAILSLRNLRILNCEGLNSLALFDWFIERFWVTLKSKSSDCN 903

Query: 905  ------------------------------------------------------------ 964
                                                                        
Sbjct: 904  LFSESIPNDFLHSSTGLTGFSRDFESPRDHLNPNDCNLLDERIPNDFRHSLTGFSRDFGS 963

Query: 965  ---------DLVGNN----------------------------------NNGVL------ 1024
                     DL+G +                                  +N ++      
Sbjct: 964  PRDLNLSDHDLLGESIPNDFMHSLTDFSRDFGSPRDPNPSDCNLLDESISNDLMHSFTDF 1023

Query: 1025 -------------------------------------PLRDLNLSDCNLEDEDIPDDMKC 1083
                                                  LRDLNLSDC+L DE IP+D+ C
Sbjct: 1024 SRDFGSSRDLNLSDCNLLDESISNDLRHSLRGLRDFGSLRDLNLSDCSLLDESIPNDLHC 1083

BLAST of Lsi08G001100 vs. ExPASy TrEMBL
Match: A0A5A7TDI7 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001270 PE=4 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 8.5e-195
Identity = 502/1327 (37.83%), Postives = 676/1327 (50.94%), Query Frame = 0

Query: 5    SSSSFPS-MKYDVFISFRGGGD--------DDKFTCQLAEELSNNGLTTFMSND------ 64
            +SS+ PS M YD FISF              + FT QL E L   G+  FM++D      
Sbjct: 3    ASSAHPSHMTYDTFISFNREQTFQAKDHLMYELFTKQLYESLCRWGMKVFMADDGGGSSG 62

Query: 65   --RFKFGDDSENIVQEAI--ILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVV 124
              +  F D   N+  + +  I  S+  +V+L++ Y+ S  CLREL KI+++  +   +V+
Sbjct: 63   DTKKVFTDYESNLNDKIVKAIEGSKTCIVILSRGYVHSTGCLRELVKIVDHINKAAYQVL 122

Query: 125  IPVLYQHHHPSCLVKELQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKR 184
                +  +H                                          +    S K 
Sbjct: 123  PLFCHDENH-----------------------------------------SYVPLDSMKY 182

Query: 185  EETDLELNLVQHDPNLFSDQDNSNVLM----EVPRWKSALEGITNLPTTIFLTEIRSHES 244
               ++E+             +N N ++    E+ RW+ A+  I +L              
Sbjct: 183  AYENIEI------------LENMNGIIIGNEELQRWREAISQICSLSGVALRPTSLMRTI 242

Query: 245  DIIKEVKEIINGVKESSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKAL 304
             +I +    +  V E   L  +  +L  M+ LL   S+D+ FIGIVGMGGIGKTTIA+ +
Sbjct: 243  KLIYDKLLHLKSVAEDRYLFEMPLRLRTMEMLLGSISDDVRFIGIVGMGGIGKTTIAQFV 302

Query: 305  YKNLQPKFCHNHCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRN-KK 364
            Y+   PKF +N CFL ++G  +VS+Q QLL ++    +  I +E  G   IK  L++ +K
Sbjct: 303  YQKFAPKFRNNCCFLHIAGSNIVSLQQQLLSQVFFLEDIKIVDEILGVNRIKHNLKSCQK 362

Query: 365  VLIVLDGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHD 424
            VL + DG+ +K QLE L G+PDW  +GSRII+TTRN+++ RQ  +K K+ EY  E L H 
Sbjct: 363  VLFIFDGISKKSQLEMLAGNPDWLPAGSRIIITTRNKDILRQTNFKDKVQEYSAELLSHT 422

Query: 425  SAFSLFCNHAFGEDHEPPDENF-------------------------------------- 484
            SA SLFC +AFGE    PDENF                                      
Sbjct: 423  SAVSLFCKYAFGEC--LPDENFKELSNEIIEKTGTLPLALVQIASSLYGQGIDVWEDTLK 482

Query: 485  ---------------RSSY---------------------------------------NK 544
                           +SSY                                        K
Sbjct: 483  SFHKLVYDNIFSHVLKSSYEGLQAESQQIFLDLACFLNGEKVDRVVEILEGFGYNSPRTK 542

Query: 545  LHLLAERNLIQISNENVHMHTLVRCMGRGIAREERET--QSRIWLRKDIRRVFHQ----- 604
            L LLA+R LI +S++ + MH L+ CMG+ I + E  T  Q+RIW R+D RR+FH+     
Sbjct: 543  LQLLADRYLIDLSDDQIQMHVLILCMGQEIVQRELGTHQQTRIWQREDARRLFHENYGLK 602

Query: 605  ------------------------------------------------------------ 664
                                                                        
Sbjct: 603  YIQGITMDLGEEEEELVLEAKSLADMIELKILQINNVQISENIDCLSNKLTLLNWPGYPS 662

Query: 665  ----------------------------EKEFSWLKEIDVSGSENLIETPDFSKVPNLQR 724
                                         K+F  LKEID S S++L+ETPD S+VPNLQR
Sbjct: 663  KYLPSTFQPPPLLELRLPGSNVIRLWNGRKKFGNLKEIDASDSKHLVETPDLSEVPNLQR 722

Query: 725  LILRNCERLRMVHPSISTLNHLVSLDMAG---LK--SFPEIEKHTKS------------- 784
            LIL+NCE L+ VH SI++L  LV LDM G   LK  SFP   K  K+             
Sbjct: 723  LILQNCETLQRVHASINSLERLVLLDMEGCVSLKRFSFPITCKRLKTLVLSYSGLEFFPE 782

Query: 785  ------NLTELHLDGTLIKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNL 844
                   LTELH+DGT I  L  S+  LT L LLNLR+C  LSSLP  I  L SL+TL L
Sbjct: 783  FGWWMEYLTELHIDGTSINQLSPSITYLTSLVLLNLRNCIGLSSLPTEICCLCSLKTLTL 842

Query: 845  NGCKSLHQIPFTLGAIQSLTMLDIGGTLIDQAPHAITSLRNLESLNCERLSRNIWWSLTC 904
            NGC+SL +IP +LG ++ L  LDIGGT I   P     L NL  LNCERL  NIW S   
Sbjct: 843  NGCESLDKIPSSLGYVEPLEELDIGGTSISTIPF----LENLRILNCERLKSNIWHS--- 902

Query: 905  DLVGNNNNGVLPLRDLNLSDCNLEDEDIPDDMKCLSLLEILDLSKNSFVRLKESLTQLIN 964
             L G   + V  L+DLNLSDCNL DEDIP+D++  S LEILDLS N F RL ES+ QLIN
Sbjct: 903  -LAGLPAHYVRSLKDLNLSDCNLVDEDIPNDLELFSSLEILDLSSNHFERLSESIEQLIN 962

Query: 965  LKALCLNDCNNIQPQLLPKLPASLRYMGGQNN-SQHHISQNHEGSYGRNSASSTSNSPRI 1024
            LKAL LNDC+ ++   +PKLP S+RY+GG+ +      SQ    S   +   + S SP  
Sbjct: 963  LKALYLNDCHKLKQ--VPKLPESIRYVGGEKSLGMLRTSQGKFYSMTGSPVCTMSPSPSN 1022

Query: 1025 VP---EIENVYDDEKEEYSTEEVDNINYGNKMGKHISDQ--LSSDSKLEVFCKDLVIGME 1084
             P     +N  ++     +T+EVD I     MGK  +D   + +D KL +  K  ++GME
Sbjct: 1023 APLSTNNDNFQNEADRFQATKEVDII---KDMGKQQTDHKLVQTDHKLVLSHKTSLVGME 1082

Query: 1085 EQLEEVHKLLDLKESNEVQFVWICGMAGIGKTTIAQVVYNEILHKFHTSCFLHISQK-NN 1089
             Q+E+V  LLDL+ S +  FV I G +GIGKTTIA+VVYN I+ +F + CFL++S K N+
Sbjct: 1083 NQMEKVCNLLDLERSKDALFVGIFGSSGIGKTTIAEVVYNTIVDQFQSGCFLYLSSKQNS 1142

BLAST of Lsi08G001100 vs. ExPASy TrEMBL
Match: A0A7L9RV91 (Resistance gene-like protein OS=Cucumis melo OX=3656 GN=Prv PE=4 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 3.6e-193
Identity = 499/1327 (37.60%), Postives = 675/1327 (50.87%), Query Frame = 0

Query: 6    SSSFPS-MKYDVFISFRGGGDDD---KFTCQLAEELSNNGLTTFMSNDRFKFGDD----- 65
            SSS PS M +DVF+SF    +DD   +F   L E LS  G+  FM +D+  F DD     
Sbjct: 5    SSSHPSQMAFDVFLSFNRDEEDDGYRRFIKCLYETLSEWGIKMFMDDDKKMFMDDIKMLK 64

Query: 66   --------------SENIVQEAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYE 125
                          S++IV+   I  S   +VVLTK Y +S  CLREL KI++ + +   
Sbjct: 65   DDGKKMFMDDEVNLSDDIVK--AIEGSITSIVVLTKGYASSKWCLRELVKIIDQKNKTKH 124

Query: 126  EVVIPVLYQH--HHPSCLVKELQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKS 185
            + V+P+ Y+H  HH   +  + QD  +      V+ +     +     ++    +   K 
Sbjct: 125  Q-VLPLFYRHLVHH---VYHQSQDVVRHQSQDVVQHQSQDVVRRQSQDVRKFFRSMAEKD 184

Query: 186  FSTKREETDLELNLVQHDPNLFSDQDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHE 245
            +S     T L ++     P ++  + NSN +                       +I+S  
Sbjct: 185  YSEVNYRTSLAMSEFCRLPGIYISRKNSNTMF----------------------QIKSTA 244

Query: 246  SDIIKEVKEIINGVKESSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKA 305
              II  +  +    KE + L  +  +L  M+ LL LGSNDI FIGIVGM GIGKTT+A+ 
Sbjct: 245  KQIIDRLLSLKLEAKEGN-LFEMPLRLRTMEMLLGLGSNDIRFIGIVGMSGIGKTTLAEV 304

Query: 306  LYKNLQPKFCHN---HCFLRVSGQKLVSMQNQLLLRLCGK--LEEVIKNEDHGAQLIKKC 365
            +Y +       +    CFL   G+ +VS+Q QLL +L     ++  I +E+HG +LIK+ 
Sbjct: 305  IYAHSFKSLISDLGKRCFLHSRGRSIVSLQQQLLDQLASSKMIDIQILDENHGVRLIKQH 364

Query: 366  LR-NKKVLIVLDGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDI 425
            LR  KKVLIVLDG+ E  QLE L GSPDWF  GSRII+TT N+++ R P +K K+ EY++
Sbjct: 365  LRFLKKVLIVLDGISETSQLEMLAGSPDWFGKGSRIIITTTNKDIFRHPNFKDKVQEYNV 424

Query: 426  EFLDHDSAFSLFCNHAFGEDHEPPDEN--------------------------------- 485
            E   H++AFSLFC  AFG+   PP E+                                 
Sbjct: 425  ELPSHEAAFSLFCKLAFGD--YPPSEDMKDLCNEIIEKVGRLPLALEKIASSLYGHDMNI 484

Query: 486  --------------------FRSSY----------------------------------- 545
                                 +SSY                                   
Sbjct: 485  WEDTLKNFHKVVYDNIFSDILKSSYEGLEAESQQIFLDLACFLNGEKVDRVIEILQGFGY 544

Query: 546  ----NKLHLLAERNLIQISNENVHMHTLVRCMGRGIAREERET--QSRIWLRKDIRRVFH 605
                  L LL +R LI I + ++ MH L+ CMG+ I R +  T  Q+RIWLR D RR+FH
Sbjct: 545  SSPQTNLQLLVDRCLIDILDGHIQMHILILCMGKEIVRRKLGTCQQTRIWLRDDARRLFH 604

Query: 606  QEKE-------------------------------------------------------- 665
            +  E                                                        
Sbjct: 605  ENNELKYICGIVMDLEEEEELVLKAKAFAGMSELKILRINNVQLSEDIEFLSNKLTLLNW 664

Query: 666  ------------------------------------FSWLKEIDVSGSENLIETPDFSKV 725
                                                F  LKEID S S+ L+ETP+FS+ 
Sbjct: 665  PGYPSKYLPSTFQPPSLIELHLPGSNVERLWNGTQKFKNLKEIDASDSKYLVETPNFSEA 724

Query: 726  PNLQRLILRNCERLRMVHPSISTLNHLVSLDM-------------------------AGL 785
             NL+RLILRNC RL+ VH SI++L+ L+  DM                          GL
Sbjct: 725  RNLRRLILRNCGRLKEVHSSINSLHRLILFDMEGCVSFKSFSFVITCESLKTLVLSNCGL 784

Query: 786  KSFPEIEKHTKSNLTELHLDGTLIKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLIS 845
            + FPE        LTELH+DGT I +L  S+  L  L LLNL +C +LSSLP  I  L S
Sbjct: 785  EFFPEF-GFPMGYLTELHIDGTSINELSPSIKNLLGLVLLNLGNCIRLSSLPTEIGSLSS 844

Query: 846  LQTLNLNGCKSLHQIPFTLGAIQSLTMLDIGGTLIDQAPHAITSLRNLESLNCERLSRNI 905
            L+TL LNGCK+LH++P +L  ++ L  LDIGGT I   P     + NL  LNCERL   I
Sbjct: 845  LKTLILNGCKNLHKLPPSLEYVKPLEELDIGGTSISTIPF----VENLRILNCERLKSII 904

Query: 906  WWSLTCDLVGNNNNGVLPLRDLNLSDCNLEDEDIPDDMKCLSLLEILDLSKNSFVRLKES 965
            W S    L          L+DLNLSDCNL DEDIP D++  S LEILDL  N F RL ES
Sbjct: 905  WHS----LASLPTEYFSSLKDLNLSDCNLVDEDIPSDLELFSSLEILDLGSNHFERLSES 964

Query: 966  LTQLINLKALCLNDCNNIQPQLLPKLPASLRYMGGQNNSQHHISQNHEGSYGRNSASSTS 1025
            + QLINLK L LNDC+ ++   +PKLP S+RY+GG+ +    +    +GS     + S+S
Sbjct: 965  IEQLINLKVLYLNDCHKLKQ--VPKLPQSIRYVGGEKSL--GMLTTSQGSPACTMSPSSS 1024

Query: 1026 NSPRIVPEIENVYDDEKEEYSTEEVDNINYGNKMGKHISDQLSSDSKLEVFCKDLVIGME 1085
            N+P +         +     +T+EVD I     MGK       +D KL +  K  ++GME
Sbjct: 1025 NAPLLT--------NNDNFQNTKEVDII---KDMGKR------TDHKLILSHKTSLVGME 1084

Query: 1086 EQLEEVHKLLDLKESNEVQFVWICGMAGIGKTTIAQVVYNEILHKFHTSCFLHISQK-NN 1089
             Q+E+V  LLDLK S +  FV I G +GIGKTTIA+VVYN I+ +F + CFL++S K N+
Sbjct: 1085 NQVEKVCNLLDLKRSKDTLFVGIFGSSGIGKTTIAEVVYNTIVDQFQSGCFLYLSSKPNS 1144

BLAST of Lsi08G001100 vs. NCBI nr
Match: KAA0039303.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 1055.8 bits (2729), Expect = 2.7e-304
Identity = 594/935 (63.53%), Postives = 686/935 (73.37%), Query Frame = 0

Query: 234  VKESSKLVGISPQLHQ-MDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHN 293
            VK   KL+GISPQLHQ MDNL++LGSND+ FIGIVGM GIGKTT+AKA+ + L+PKF +N
Sbjct: 4    VKGPPKLIGISPQLHQIMDNLVELGSNDVGFIGIVGMSGIGKTTLAKAVCQELEPKFGYN 63

Query: 294  -HCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEK 353
             HCFLRV  +KLV++Q +LLLR  GKL+ VIKNEDHGA+LIKKCLRNKKVLIVLDGVDE 
Sbjct: 64   SHCFLRVHTEKLVTVQKKLLLRFGGKLDIVIKNEDHGAKLIKKCLRNKKVLIVLDGVDEI 123

Query: 354  RQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAF 413
            RQ++KL GSPDWFS GSRII+TTRNRNLCRQ KYKHKIFEYD+E LDH+SAF LFCNHAF
Sbjct: 124  RQMKKLAGSPDWFSPGSRIIITTRNRNLCRQQKYKHKIFEYDVECLDHNSAFLLFCNHAF 183

Query: 414  GED-HEPPDENFRSSYNKLHLLAERNLIQISNE-----------------------NVHM 473
            G+D HEPP+E  R    ++ L   + ++ +  E                       NV +
Sbjct: 184  GKDVHEPPNETLRYLTEEMGLKYVKGIVIMDMEDKQLLLDATPFAYMTELKILKMNNVKV 243

Query: 474  HTLVRCMGRGI---------------AREERETQSRIWLRKDIRRVFHQEKEFSWLKEID 533
            +  +R +   +                 E R           + +++  +KEF WLKEID
Sbjct: 244  YGDIRFLSNELRFLYWNGYPDYCLPFTFEPRHILELHLRNSQLDQLWEGQKEFIWLKEID 303

Query: 534  VSGSENLIETPDFSKVPNLQRLILRNCERLRMVHPSISTLNHLVSLDMA----------- 593
            VSGSE L+ETPDFSKVPNLQRLILRNCERL  VHPSI+TLNHL+ +DMA           
Sbjct: 304  VSGSEYLVETPDFSKVPNLQRLILRNCERLCEVHPSITTLNHLLLVDMANCVNLKTFPPK 363

Query: 594  ---------------GLKSFPEIEKHTKSNLTELHLDGTLIKDLPFSLGLLTHLALLNLR 653
                           G++SFPEIE+ TKS LTELHLDG LI D                 
Sbjct: 364  LITSKRLQTLILSNSGIESFPEIEEPTKS-LTELHLDGMLIHD----------------- 423

Query: 654  DCTKLSSLPRSIRKLISLQTLNLNGCKSLHQIPFTLGAIQSLTMLDIGGTLIDQAPHAIT 713
                   LP SI +L+SLQTLNLNGCKSLHQIP TL  I SLTMLDIGGTLID AP+AIT
Sbjct: 424  -------LPESICRLMSLQTLNLNGCKSLHQIPSTLETISSLTMLDIGGTLIDHAPNAIT 483

Query: 714  SLRNLESLNCERLSRNIWWSLTCDLVGNNNNGVLPLRDLNLSDCNLEDEDIPDDMKCLSL 773
            S  NLE+LNCE+LSRNIW SL+  LVG  NNGVLPLRDLNLS C L+DEDIPDD+ CLS 
Sbjct: 484  SFSNLETLNCEKLSRNIWKSLS-GLVG-INNGVLPLRDLNLSACKLDDEDIPDDLNCLSS 543

Query: 774  LEILDLSKNSFVRLKESLTQLINLKALCLNDCNNIQPQLLPKLPASLRYMGGQNNSQHHI 833
            LEILDLS NSFV+LKESLTQL NLKAL LNDCN+IQPQLLPK   +L+Y+GG  NS   I
Sbjct: 544  LEILDLSNNSFVKLKESLTQLTNLKALYLNDCNDIQPQLLPKFLTTLQYVGGL-NSLKSI 603

Query: 834  SQNHEGSYGRNSASSTSNSPRIVPEIENVYDDEKEEYSTEEVDNINYGNKMGKHISDQLS 893
            SQN EGS G  SA    + P+ V EIEN+  + +EE S EEVDNI    KMGK IS QL+
Sbjct: 604  SQNDEGSSGSKSACPPFSPPKTVTEIENIVYEIEEEKSHEEVDNIKV-KKMGKQIS-QLN 663

Query: 894  SDSKLEVFCKDLVIGMEEQLEEVHKLLDLKESNEVQFVWICGMAGIGKTTIAQVVYNEIL 953
            + SK    C   ++GM+EQLEEV KLLDLKES EVQFVWICGM GIGKTTIA+VVYNEI+
Sbjct: 664  THSKFGKVCGKELVGMKEQLEEVLKLLDLKESTEVQFVWICGMDGIGKTTIAEVVYNEIV 723

Query: 954  HKFHTSCFLHISQKNNLVSLQHQLLSQLLLKDDIEVRDEDYGANMIENQMKSRMVLIVLD 1013
             KF +SCFLH++Q+ NLVSLQHQLLS+LLLKDDIE+RDED GA MIEN+MKSRM L+VLD
Sbjct: 724  GKFESSCFLHVNQRKNLVSLQHQLLSRLLLKDDIEIRDEDRGAQMIENEMKSRMALVVLD 783

Query: 1014 DIDEKRQVEVLVGSPDWFGQGSRVIITTRNRDILHQLNYKDKIREYNVKLLSQESANLLF 1073
            DIDEKRQVEVLVGSPDWFG GSR+IITTRNR+I+HQLN+KDK+REYNVKLLSQE+ANLLF
Sbjct: 784  DIDEKRQVEVLVGSPDWFGHGSRIIITTRNREIIHQLNHKDKMREYNVKLLSQENANLLF 843

Query: 1074 CKYAFGSYYDGQDKNDDVLCSEIVKRVGRLPLALILIGSALHNKDEDIWKETLRILCNVE 1102
            CKYAF +  + Q  N D LC+EIVK+VGRLPLALILIGSAL NKD   WKE LRILCNVE
Sbjct: 844  CKYAFENNNNAQHTN-DALCTEIVKKVGRLPLALILIGSALRNKDAAKWKEMLRILCNVE 903

BLAST of Lsi08G001100 vs. NCBI nr
Match: XP_038890297.1 (TMV resistance protein N-like [Benincasa hispida])

HSP 1 Score: 823.5 bits (2126), Expect = 2.3e-234
Identity = 508/940 (54.04%), Postives = 572/940 (60.85%), Query Frame = 0

Query: 1   MNPPSSSSFPSMKYDVFISFRG---GGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDS 60
           M P S+SSFP   YDVFIS RG   GGDD KF  +LA+ELSN GL T+   + FK G DS
Sbjct: 14  MEPSSTSSFPK-TYDVFISSRGGSSGGDDSKFASELAKELSNIGLRTY---EGFKLGGDS 73

Query: 61  ENIVQEAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSC 120
           + I+QE  I NSR+ +VVLTKDY+TSIRCLRELS+IME RGEDYEEV+   ++   HPSC
Sbjct: 74  Q-IMQEEAIKNSRHFIVVLTKDYVTSIRCLRELSRIMESRGEDYEEVI--TVFYKDHPSC 133

Query: 121 LVKELQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQH 180
           LVKEL+DFGKRLED+K   K+                                       
Sbjct: 134 LVKELEDFGKRLEDHKKEVKQ--------------------------------------- 193

Query: 181 DPNLFSDQDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGVKES 240
                       +LME+PR     +   NLP TIF+ E R H+     +V EIIN VKES
Sbjct: 194 -----------KLLMELPRCTLLWKDSANLPKTIFVEEKRYHQ-----QVNEIINKVKES 253

Query: 241 SKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNHCFLR 300
           S +VGISPQLHQ+D+LLDL SND+ FIGIVGMGGI                         
Sbjct: 254 STIVGISPQLHQVDDLLDLDSNDVRFIGIVGMGGI------------------------- 313

Query: 301 VSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLEKL 360
                 VS+Q +LLLRL GKL++VIKNEDHGA+LIKKCLRNKK LIVLDGVDE+RQ++KL
Sbjct: 314 ------VSVQKKLLLRLGGKLDKVIKNEDHGAKLIKKCLRNKKFLIVLDGVDERRQIKKL 373

Query: 361 VGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAFGEDHEP 420
             SPDWF+ GSRIIVTTRNRNLC           Y++EFLDHD         AFGEDHEP
Sbjct: 374 ARSPDWFNPGSRIIVTTRNRNLC-----------YNVEFLDHD---------AFGEDHEP 433

Query: 421 PDENFR----------------------------------------------SSYNKLHL 480
           PDENFR                                              SS+ KLHL
Sbjct: 434 PDENFRDLAEEMTSYNGLDDDSQQIFLDLACFFNNGMSVDRVIEILESFGYKSSHKKLHL 493

Query: 481 LAERNLIQISNENVHMHTLVRCMGRGIAREERETQSRIWLRKDIRRVFHQE--------- 540
           LAERNLIQIS + V MHTLVRCMGRG  R +RETQ+RIWLRKD+RRV+H+E         
Sbjct: 494 LAERNLIQISYDMVQMHTLVRCMGRGNVRRKRETQTRIWLRKDLRRVYHEESGLKHVHGI 553

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 554 VVVDMEDEELELEAKPFAYMTELKLLEMNNVKVDGNIELQLSNKLRFLNWNGYPFKHLPS 613

Query: 601 ------------------------KEFSWLKEIDVSGSENLIETPDFSKVPNLQRLILRN 660
                                   KEF WLKEIDVSGSENL ETPDFS VPNLQRLILRN
Sbjct: 614 NFEPQSLLELHLRNSNLMRLWEGKKEFIWLKEIDVSGSENLDETPDFSNVPNLQRLILRN 673

Query: 661 CERLRMVHPSISTLNHLVSLDMA--------------------------GLKSFPEIEKH 720
           C RL +VHPSI+TLNHLV +DMA                          GL+ FPEIEK 
Sbjct: 674 CGRLCVVHPSITTLNHLVLVDMANCLNLKIFPSKLITCKRLQTLILSNSGLECFPEIEKP 733

Query: 721 TKSNLTELHLDGTLIKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNLNGC 772
           TKS LTELHLDGTLI+DLP S GLLTHL LLNLRDCT+L SLPRSIRKLISLQTLNLNGC
Sbjct: 734 TKS-LTELHLDGTLIQDLPLSFGLLTHLTLLNLRDCTQLCSLPRSIRKLISLQTLNLNGC 793

BLAST of Lsi08G001100 vs. NCBI nr
Match: XP_008465659.2 (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 718.8 bits (1854), Expect = 7.9e-203
Identity = 445/850 (52.35%), Postives = 503/850 (59.18%), Query Frame = 0

Query: 1   MNPPS---SSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDS 60
           MNPPS   +SS P     VFISF     D KF  +LA+E  N  L TFMS+     GDD 
Sbjct: 1   MNPPSTSPTSSSPPNTCQVFISFATA--DSKFVSELAKEFRNIRLRTFMSS-----GDDG 60

Query: 61  ENIVQEAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSC 120
           E I+ E  +  SR+ VVVLTKDY+ SI+CLRELS+IMEY+ E YEE VIPV YQ   PSC
Sbjct: 61  E-IIHEDAMKKSRHFVVVLTKDYVNSIKCLRELSRIMEYKDEGYEE-VIPVFYQ--DPSC 120

Query: 121 LVKELQDFGKRLEDY-KVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQ 180
           LVKELQDFGKRLEDY ++  +EG    ++C FLKT L  + NKSFS K E          
Sbjct: 121 LVKELQDFGKRLEDYEEIIKEEGLIEFTHCWFLKTDLAKRLNKSFSEKEE---------- 180

Query: 181 HDPNLFSDQDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGVKE 240
                    + SN+LMEVPRWK ALE I NLP +IF+ E  S ESD+IKEV EII  VK 
Sbjct: 181 ---------NKSNLLMEVPRWKFALERINNLPKSIFVGE-ESLESDVIKEVTEIIYKVKA 240

Query: 241 SSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNHCFL 300
           S KLVGISP LHQMDNLL+LGSNDI FIGIVG+GGIGKTTIAK LY+ L+ KF +  CFL
Sbjct: 241 SPKLVGISPPLHQMDNLLNLGSNDIRFIGIVGLGGIGKTTIAKVLYEKLEHKFRY-CCFL 300

Query: 301 RVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLEK 360
           R   QKLVS+Q +LLLRLCGK + VIKNEDHGA+LIKKCLRNKKVLIVLDGVDE+RQ+EK
Sbjct: 301 RDFDQKLVSIQKKLLLRLCGKFDIVIKNEDHGAKLIKKCLRNKKVLIVLDGVDERRQIEK 360

Query: 361 LVG-SPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAFGEDH 420
           LVG S DWFS GSRII+TTRNRNLC QPKYKHK+ EY++EFLDH +AFSLFCNHAFGE+H
Sbjct: 361 LVGSSQDWFSPGSRIIITTRNRNLCCQPKYKHKMLEYNVEFLDHHNAFSLFCNHAFGENH 420

Query: 421 EPPDENF----------------------------------------------------- 480
           EP DENF                                                     
Sbjct: 421 EPLDENFRYLAEEMVKMVEGHPLALIKIGSHLHGKDIDKWEESLEKVHKLLYKYLFCEVF 480

Query: 481 ----------------------------------------RSSYNKLHLLAERNLIQISN 540
                                                   RS YNKL+LL +RNLIQ+S+
Sbjct: 481 RTSYEELDDESQQVFLDLACFFNNGMSMDRAIEILESFGYRSPYNKLNLLTQRNLIQVSH 540

Query: 541 ENVHMHTLVRCMGRGIAREERETQSRIWLRKDIRRVFHQE-------------------- 600
             V MHTLV CMGRGI + ERETQSRIWLRK IR +F +E                    
Sbjct: 541 GMVQMHTLVHCMGRGIVQGERETQSRIWLRKHIRHMFGKEKGLEDVEGIVVMDMEEEELV 600

Query: 601 ------------------------------------------------------------ 635
                                                                       
Sbjct: 601 LDAKSFAYMNKLKLLEINNVRVDEDIQFLSNKLGILRWNGYPSKYLPSTFQPQSLLELHL 660

BLAST of Lsi08G001100 vs. NCBI nr
Match: XP_022141839.1 (LOW QUALITY PROTEIN: TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 701.0 bits (1808), Expect = 1.7e-197
Identity = 528/1462 (36.11%), Postives = 709/1462 (48.50%), Query Frame = 0

Query: 5    SSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFK--FGDDSENIVQ 64
            S S  P M +DVF+SF    + + FT  L   L +         D+ K  F D+    + 
Sbjct: 4    SLSHPPQMSFDVFLSF--SKEYELFTANLCRSLRSRLRIRIFMEDKTKLSFEDNLSKKIV 63

Query: 65   EAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKEL 124
            EA I  S   VVVL+K Y +S  CL+EL+KI+++  E+   +V+PV              
Sbjct: 64   EA-IEGSMCSVVVLSKHYASSKSCLKELAKIIDHM-ENTTHLVLPVF------------- 123

Query: 125  QDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNLF 184
                K ++   VR + G   KS            F++   + +E              L 
Sbjct: 124  ----KDVDPIHVRHQSGNLKKS------------FDEYEKSAQE--------------LM 183

Query: 185  SDQDNSNVLMEVPRWKSALEGITNL--PTTIFLTEIRSHESDIIKEVKEIINGVK---ES 244
             ++ N   L E+ RW+ AL  I NL  P T  ++ +    S I K++ ++++ +K   E+
Sbjct: 184  DNEQNEKHLKEIERWRDALTKIANLSGPATKLVSGVIG-ISRITKQISDMLHHLKLVAEN 243

Query: 245  SKLVGISPQLHQMDNLL-DLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNHCFL 304
              LV + P+L +M+ LL +L SND+ F+ IVGMGGIGKTTIA+  Y  L PKF  + CFL
Sbjct: 244  RYLVEMPPRLGRMNMLLGNLESNDVRFVAIVGMGGIGKTTIAELFYDQLAPKFMKS-CFL 303

Query: 305  RVSGQKLVSMQNQLLLR-LCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLE 364
            R++G  LVS+Q QLL + L  K    I NE+ G + I++ L+ ++VLIVLDG+ EK QL+
Sbjct: 304  RIAGCNLVSLQQQLLSQLLLSKTNIHILNENDGVRKIQRHLKFERVLIVLDGIHEKMQLK 363

Query: 365  KLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAFGEDH 424
             L G+P WF   SR+I+TTRN+++   P Y+ K+ EY++E L   +A SL C HAFG+D 
Sbjct: 364  MLAGNPYWFGRKSRVIITTRNKDIFHHPNYRDKVQEYNVELLHDTTAHSLLCKHAFGDD- 423

Query: 425  EPPDENFR---------------------------------------------------- 484
            EPP+E F+                                                    
Sbjct: 424  EPPNETFKDLCNEIIEKVGRLPLALIKIGSSLYGQTMDIWEGALKSYHKLVYENLFSAIL 483

Query: 485  ----------------------------------------SSYNKLHLLAERNLIQISNE 544
                                                    S + KL LL +R LI +S+E
Sbjct: 484  KTSYEGLEVESQQIFLDCACFFNGEKVDRVVEILESFGYSSPHTKLQLLVDRCLIDVSDE 543

Query: 545  NVHMHTLVRCMGRGIA-REERETQSRIWLRKDIRRVFHQE-------------------- 604
             + MH L+ CMGRGI    ++  QSRIWLR+D  RVFH++                    
Sbjct: 544  RIQMHNLILCMGRGIVLATQQGKQSRIWLREDAHRVFHEKSELKYIQGIVMDLEEGEELV 603

Query: 605  ------------------------------------------------------------ 664
                                                                        
Sbjct: 604  VEAKSFTDMTELKILQINNVELSEDIEFLSNQLRLLHWPGYPSKCLPSTFQPPSLLELHL 663

Query: 665  ------------KEFSWLKEIDVSGSENLIETPDFSKVPNLQRLILRNCERLRMVHPSIS 724
                        K+F  LKEIDVS S++L+ETPDFS+VPNL+RLILRNC +L +VHPSI+
Sbjct: 664  HGSKLERLWEGRKKFEKLKEIDVSESKHLVETPDFSEVPNLRRLILRNCGKLDVVHPSIN 723

Query: 725  TLNHLVSLDM--------------------------AGLKSFPEIEKHTKSNLTELHLDG 784
            +L  LV LD+                          +GL+ FPE+ +     LTELHL+G
Sbjct: 724  SLERLVLLDLEGCISLRSFSSNYINFKSLKTLMLSNSGLECFPEVGE-PMDRLTELHLEG 783

Query: 785  TLIKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNLNGCKSLHQIPFTLGA 844
            T+I +LP S+  LT L LLNLR+CT LSSLP  I  L +L+TL LNGCK L +IP  LG 
Sbjct: 784  TIINELPLSIRHLTSLVLLNLRNCTGLSSLPSEIGSLSNLKTLILNGCKELKEIPPNLGD 843

Query: 845  IQSLTMLDIGGTLIDQAPHAITSLRNLESLNCERLS---------RNIWWSLTC------ 904
            ++SL  LDIG T I QAPHAI SLRNL  LNCE L+            W +L        
Sbjct: 844  VESLEELDIGRTSISQAPHAILSLRNLRILNCEGLNSLALFDWFIERFWVTLKSKSSDCN 903

Query: 905  ------------------------------------------------------------ 964
                                                                        
Sbjct: 904  LFSESIPNDFLHSSTGLTGFSRDFESPRDHLNPNDCNLLDERIPNDFRHSLTGFSRDFGS 963

Query: 965  ---------DLVGNN----------------------------------NNGVL------ 1024
                     DL+G +                                  +N ++      
Sbjct: 964  PRDLNLSDHDLLGESIPNDFMHSLTDFSRDFGSPRDPNPSDCNLLDESISNDLMHSFTDF 1023

Query: 1025 -------------------------------------PLRDLNLSDCNLEDEDIPDDMKC 1083
                                                  LRDLNLSDC+L DE IP+D+ C
Sbjct: 1024 SRDFGSSRDLNLSDCNLLDESISNDLRHSLRGLRDFGSLRDLNLSDCSLLDESIPNDLHC 1083

BLAST of Lsi08G001100 vs. NCBI nr
Match: KAA0039329.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 691.0 bits (1782), Expect = 1.8e-194
Identity = 502/1327 (37.83%), Postives = 676/1327 (50.94%), Query Frame = 0

Query: 5    SSSSFPS-MKYDVFISFRGGGD--------DDKFTCQLAEELSNNGLTTFMSND------ 64
            +SS+ PS M YD FISF              + FT QL E L   G+  FM++D      
Sbjct: 3    ASSAHPSHMTYDTFISFNREQTFQAKDHLMYELFTKQLYESLCRWGMKVFMADDGGGSSG 62

Query: 65   --RFKFGDDSENIVQEAI--ILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVV 124
              +  F D   N+  + +  I  S+  +V+L++ Y+ S  CLREL KI+++  +   +V+
Sbjct: 63   DTKKVFTDYESNLNDKIVKAIEGSKTCIVILSRGYVHSTGCLRELVKIVDHINKAAYQVL 122

Query: 125  IPVLYQHHHPSCLVKELQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKR 184
                +  +H                                          +    S K 
Sbjct: 123  PLFCHDENH-----------------------------------------SYVPLDSMKY 182

Query: 185  EETDLELNLVQHDPNLFSDQDNSNVLM----EVPRWKSALEGITNLPTTIFLTEIRSHES 244
               ++E+             +N N ++    E+ RW+ A+  I +L              
Sbjct: 183  AYENIEI------------LENMNGIIIGNEELQRWREAISQICSLSGVALRPTSLMRTI 242

Query: 245  DIIKEVKEIINGVKESSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKAL 304
             +I +    +  V E   L  +  +L  M+ LL   S+D+ FIGIVGMGGIGKTTIA+ +
Sbjct: 243  KLIYDKLLHLKSVAEDRYLFEMPLRLRTMEMLLGSISDDVRFIGIVGMGGIGKTTIAQFV 302

Query: 305  YKNLQPKFCHNHCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRN-KK 364
            Y+   PKF +N CFL ++G  +VS+Q QLL ++    +  I +E  G   IK  L++ +K
Sbjct: 303  YQKFAPKFRNNCCFLHIAGSNIVSLQQQLLSQVFFLEDIKIVDEILGVNRIKHNLKSCQK 362

Query: 365  VLIVLDGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHD 424
            VL + DG+ +K QLE L G+PDW  +GSRII+TTRN+++ RQ  +K K+ EY  E L H 
Sbjct: 363  VLFIFDGISKKSQLEMLAGNPDWLPAGSRIIITTRNKDILRQTNFKDKVQEYSAELLSHT 422

Query: 425  SAFSLFCNHAFGEDHEPPDENF-------------------------------------- 484
            SA SLFC +AFGE    PDENF                                      
Sbjct: 423  SAVSLFCKYAFGEC--LPDENFKELSNEIIEKTGTLPLALVQIASSLYGQGIDVWEDTLK 482

Query: 485  ---------------RSSY---------------------------------------NK 544
                           +SSY                                        K
Sbjct: 483  SFHKLVYDNIFSHVLKSSYEGLQAESQQIFLDLACFLNGEKVDRVVEILEGFGYNSPRTK 542

Query: 545  LHLLAERNLIQISNENVHMHTLVRCMGRGIAREERET--QSRIWLRKDIRRVFHQ----- 604
            L LLA+R LI +S++ + MH L+ CMG+ I + E  T  Q+RIW R+D RR+FH+     
Sbjct: 543  LQLLADRYLIDLSDDQIQMHVLILCMGQEIVQRELGTHQQTRIWQREDARRLFHENYGLK 602

Query: 605  ------------------------------------------------------------ 664
                                                                        
Sbjct: 603  YIQGITMDLGEEEEELVLEAKSLADMIELKILQINNVQISENIDCLSNKLTLLNWPGYPS 662

Query: 665  ----------------------------EKEFSWLKEIDVSGSENLIETPDFSKVPNLQR 724
                                         K+F  LKEID S S++L+ETPD S+VPNLQR
Sbjct: 663  KYLPSTFQPPPLLELRLPGSNVIRLWNGRKKFGNLKEIDASDSKHLVETPDLSEVPNLQR 722

Query: 725  LILRNCERLRMVHPSISTLNHLVSLDMAG---LK--SFPEIEKHTKS------------- 784
            LIL+NCE L+ VH SI++L  LV LDM G   LK  SFP   K  K+             
Sbjct: 723  LILQNCETLQRVHASINSLERLVLLDMEGCVSLKRFSFPITCKRLKTLVLSYSGLEFFPE 782

Query: 785  ------NLTELHLDGTLIKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNL 844
                   LTELH+DGT I  L  S+  LT L LLNLR+C  LSSLP  I  L SL+TL L
Sbjct: 783  FGWWMEYLTELHIDGTSINQLSPSITYLTSLVLLNLRNCIGLSSLPTEICCLCSLKTLTL 842

Query: 845  NGCKSLHQIPFTLGAIQSLTMLDIGGTLIDQAPHAITSLRNLESLNCERLSRNIWWSLTC 904
            NGC+SL +IP +LG ++ L  LDIGGT I   P     L NL  LNCERL  NIW S   
Sbjct: 843  NGCESLDKIPSSLGYVEPLEELDIGGTSISTIPF----LENLRILNCERLKSNIWHS--- 902

Query: 905  DLVGNNNNGVLPLRDLNLSDCNLEDEDIPDDMKCLSLLEILDLSKNSFVRLKESLTQLIN 964
             L G   + V  L+DLNLSDCNL DEDIP+D++  S LEILDLS N F RL ES+ QLIN
Sbjct: 903  -LAGLPAHYVRSLKDLNLSDCNLVDEDIPNDLELFSSLEILDLSSNHFERLSESIEQLIN 962

Query: 965  LKALCLNDCNNIQPQLLPKLPASLRYMGGQNN-SQHHISQNHEGSYGRNSASSTSNSPRI 1024
            LKAL LNDC+ ++   +PKLP S+RY+GG+ +      SQ    S   +   + S SP  
Sbjct: 963  LKALYLNDCHKLKQ--VPKLPESIRYVGGEKSLGMLRTSQGKFYSMTGSPVCTMSPSPSN 1022

Query: 1025 VP---EIENVYDDEKEEYSTEEVDNINYGNKMGKHISDQ--LSSDSKLEVFCKDLVIGME 1084
             P     +N  ++     +T+EVD I     MGK  +D   + +D KL +  K  ++GME
Sbjct: 1023 APLSTNNDNFQNEADRFQATKEVDII---KDMGKQQTDHKLVQTDHKLVLSHKTSLVGME 1082

Query: 1085 EQLEEVHKLLDLKESNEVQFVWICGMAGIGKTTIAQVVYNEILHKFHTSCFLHISQK-NN 1089
             Q+E+V  LLDL+ S +  FV I G +GIGKTTIA+VVYN I+ +F + CFL++S K N+
Sbjct: 1083 NQMEKVCNLLDLERSKDALFVGIFGSSGIGKTTIAEVVYNTIVDQFQSGCFLYLSSKQNS 1142

BLAST of Lsi08G001100 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 209.5 bits (532), Expect = 1.4e-53
Identity = 243/913 (26.62%), Postives = 371/913 (40.64%), Query Frame = 0

Query: 14  YDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIVQEAIILNSRYLV 73
           YDVF+SFRG      F   L + L   G++TFM +   + G+     +  A I  S+ L+
Sbjct: 14  YDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNA-IETSKILI 73

Query: 74  VVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKELQDFGKRLEDYK 133
           VVLTKDY +S  CL EL  IM+    +   +V P+ + +  PS                 
Sbjct: 74  VVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPI-FLYVDPS----------------D 133

Query: 134 VRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNLFSDQDNSNVLME 193
           +R ++G + KS                                     FS   NS+ L +
Sbjct: 134 IRWQQGSYAKS-------------------------------------FSKHKNSHPLNK 193

Query: 194 VPRWKSALEGITNLPTTIFLTEIRS-HESDIIKEV-KEIING-----VKESSKLVGISPQ 253
           +  W+ AL  + N+       +I++ +E++ I ++ +EI+       +   S  VG+  +
Sbjct: 194 LKDWREALTKVANISG----WDIKNRNEAECIADITREILKRLPCQYLHVPSYAVGLRSR 253

Query: 254 LHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQ-----PKFCHNHCFLRVSGQ 313
           L  + +LL +GS+ +  I I GMGGIGKTT+AK  +           F  N        +
Sbjct: 254 LQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPE 313

Query: 314 KLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLEKLVGSP 373
               +Q+QLL  +  + +   K  DH    +K+  R+K+VL+V+D VD+  QL       
Sbjct: 314 GRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQLNSAAIDR 373

Query: 374 DWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAFGEDHEP---- 433
           D F  GSRII+TTRN +L +Q + +     Y  + LD D +  LF  HAF     P    
Sbjct: 374 DCFGHGSRIIITTRNMHLLKQLRAEG---SYSPKELDGDESLELFSWHAFRTSEPPKEFL 433

Query: 434 ------------------------------------------PDENFRS----SYNK--- 493
                                                     P++N ++    S+N    
Sbjct: 434 QHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTI 493

Query: 494 -----------------------------------LHLLAERNLIQISNENVHMHTLVRC 553
                                              L LL ER LI IS  N+ MH L+R 
Sbjct: 494 EQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRD 553

Query: 554 MGRGIARE----ERETQSRIWLRKDIRRVFHQEKEFSWLKEIDVSGSE---NLIETPDFS 613
           MGR I RE    +   +SR+W   D+  V  ++   + ++ + +          E   F+
Sbjct: 554 MGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFA 613

Query: 614 KVPNLQRLILRNCE----------RLRMV---------HPSISTLNHLVSLDM--AGLKS 673
           K+  L+ L LR  +           LR +          P   +L  L +LD+  + LK 
Sbjct: 614 KMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQYSNLKR 673

Query: 674 FPEIEKHTKSNLTELHLD---GTLIKDLP-----------------------FSLGLL-T 733
           F + +   +      +LD      +++ P                        S+G+L  
Sbjct: 674 FWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDK 733

Query: 734 HLALLNLRDCTKLSSLPRSIRKLISLQTLNLNGCKSLHQIPFTLGAIQSLTMLDIGGTLI 757
            L LLNL  C +L  LP  I KL SL++L L+ C  L ++   LG ++SLT L    T +
Sbjct: 734 KLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGELESLTTLLADFTAL 793

BLAST of Lsi08G001100 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 209.5 bits (532), Expect = 1.4e-53
Identity = 243/913 (26.62%), Postives = 371/913 (40.64%), Query Frame = 0

Query: 14  YDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIVQEAIILNSRYLV 73
           YDVF+SFRG      F   L + L   G++TFM +   + G+     +  A I  S+ L+
Sbjct: 17  YDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNA-IETSKILI 76

Query: 74  VVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKELQDFGKRLEDYK 133
           VVLTKDY +S  CL EL  IM+    +   +V P+ + +  PS                 
Sbjct: 77  VVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPI-FLYVDPS----------------D 136

Query: 134 VRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNLFSDQDNSNVLME 193
           +R ++G + KS                                     FS   NS+ L +
Sbjct: 137 IRWQQGSYAKS-------------------------------------FSKHKNSHPLNK 196

Query: 194 VPRWKSALEGITNLPTTIFLTEIRS-HESDIIKEV-KEIING-----VKESSKLVGISPQ 253
           +  W+ AL  + N+       +I++ +E++ I ++ +EI+       +   S  VG+  +
Sbjct: 197 LKDWREALTKVANISG----WDIKNRNEAECIADITREILKRLPCQYLHVPSYAVGLRSR 256

Query: 254 LHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQ-----PKFCHNHCFLRVSGQ 313
           L  + +LL +GS+ +  I I GMGGIGKTT+AK  +           F  N        +
Sbjct: 257 LQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPE 316

Query: 314 KLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLEKLVGSP 373
               +Q+QLL  +  + +   K  DH    +K+  R+K+VL+V+D VD+  QL       
Sbjct: 317 GRTHLQHQLLSDILRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQLNSAAIDR 376

Query: 374 DWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSAFSLFCNHAFGEDHEP---- 433
           D F  GSRII+TTRN +L +Q + +     Y  + LD D +  LF  HAF     P    
Sbjct: 377 DCFGHGSRIIITTRNMHLLKQLRAEG---SYSPKELDGDESLELFSWHAFRTSEPPKEFL 436

Query: 434 ------------------------------------------PDENFRS----SYNK--- 493
                                                     P++N ++    S+N    
Sbjct: 437 QHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTI 496

Query: 494 -----------------------------------LHLLAERNLIQISNENVHMHTLVRC 553
                                              L LL ER LI IS  N+ MH L+R 
Sbjct: 497 EQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRD 556

Query: 554 MGRGIARE----ERETQSRIWLRKDIRRVFHQEKEFSWLKEIDVSGSE---NLIETPDFS 613
           MGR I RE    +   +SR+W   D+  V  ++   + ++ + +          E   F+
Sbjct: 557 MGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFA 616

Query: 614 KVPNLQRLILRNCE----------RLRMV---------HPSISTLNHLVSLDM--AGLKS 673
           K+  L+ L LR  +           LR +          P   +L  L +LD+  + LK 
Sbjct: 617 KMQELRLLELRYVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQYSNLKR 676

Query: 674 FPEIEKHTKSNLTELHLD---GTLIKDLP-----------------------FSLGLL-T 733
           F + +   +      +LD      +++ P                        S+G+L  
Sbjct: 677 FWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDK 736

Query: 734 HLALLNLRDCTKLSSLPRSIRKLISLQTLNLNGCKSLHQIPFTLGAIQSLTMLDIGGTLI 757
            L LLNL  C +L  LP  I KL SL++L L+ C  L ++   LG ++SLT L    T +
Sbjct: 737 KLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGELESLTTLLADFTAL 796

BLAST of Lsi08G001100 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 189.1 bits (479), Expect = 2.0e-47
Identity = 238/968 (24.59%), Postives = 369/968 (38.12%), Query Frame = 0

Query: 5   SSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIVQEA 64
           SSSS    K DVF+SFRG      F   L  E    G+  F  +   + G      + +A
Sbjct: 9   SSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELIDA 68

Query: 65  IILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKELQD 124
            I  SR+ +VV++++Y  S  CL EL KIME      ++ ++P+ Y+   PS + ++   
Sbjct: 69  -IKGSRFAIVVVSRNYAASSWCLDELLKIMECN----KDTIVPIFYE-VDPSDVRRQRGS 128

Query: 125 FGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNLFSD 184
           FG+ +E +  + K G++ ++    LK KL A                           S 
Sbjct: 129 FGEDVESHSDKEKVGKWKEA----LK-KLAA--------------------------ISG 188

Query: 185 QDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGVKESSKLVGIS 244
           +D+ N       W  +            + +I    SD     K +     +S  L+G+S
Sbjct: 189 EDSRN-------WDDSK----------LIKKIVKDISD-----KLVSTSWDDSKGLIGMS 248

Query: 245 PQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKFCHNHCFLR-----VS 304
             +  + +++ +   D+  +GI GMGG+GKTTIAK LY  L  +F   HCF+       +
Sbjct: 249 SHMDFLQSMISIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQF-QVHCFMENVKEVCN 308

Query: 305 GQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVLIVLDGVDEKRQLEKLVG 364
              +  +Q + L R+  + ++   +      +IK+  R+K V IVLD VD   QL +LV 
Sbjct: 309 RYGVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVK 368

Query: 365 SPDWFSSGSRIIVTTRNRNLCRQPKYKHKI-FEYDIEFLDHDSAFSLFCNHAFGED---- 424
              WF  GSRIIVTTR+R+L       H I   Y ++ L    A  LFCN+AF E+    
Sbjct: 369 ETGWFGPGSRIIVTTRDRHLL----LSHGINLVYKVKCLPKKEALQLFCNYAFREEIILP 428

Query: 425 -----------------------------------------------HEPPDENFRSSYN 484
                                                          H    E  R SY+
Sbjct: 429 HGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYD 488

Query: 485 KLH--------------------------------------LLAERNLIQISNENVHMHT 544
            L                                       +L E++LI  SN  V +H 
Sbjct: 489 GLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILTEKSLIVESNGCVKIHD 548

Query: 545 LVRCMGRGIAREERETQSR----IWLRKD-----------------------IRRVFHQE 604
           L+  MGR + R++          +W  +D                       I  VF  +
Sbjct: 549 LLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEGISLNLSEISEVFASD 608

Query: 605 KEFS-------------------------------------------------------- 664
           + F                                                         
Sbjct: 609 RAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFL 668

Query: 665 -------------W--------LKEIDVSGSENLIETPDFSKVPNLQRLILRNCERLRMV 724
                        W        LK++D+S  + L+E PD SK  NL+ L L  C+ L  V
Sbjct: 669 VELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEV 728

Query: 725 HPSISTLNH--------------------LVSLDMAG------LKSFPEIEKHTKSNLTE 734
            PSI  L                      L SL+  G      LK FPEI  +T+     
Sbjct: 729 TPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVGMSGCSSLKHFPEISWNTR----R 788

BLAST of Lsi08G001100 vs. TAIR 10
Match: AT3G44480.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 178.3 bits (451), Expect = 3.6e-44
Identity = 228/983 (23.19%), Postives = 383/983 (38.96%), Query Frame = 0

Query: 4   PSSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIVQE 63
           P++S   + K+DVF SF G      F   + E     G+ TF+ N+     + S++I  E
Sbjct: 88  PATSVSRNWKHDVFPSFHGADVRRTFLSHIMESFRRKGIDTFIDNNI----ERSKSIGPE 147

Query: 64  --AIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKE 123
               I  S+  +V+L++ Y +S  CL EL++IM+ R +   ++V+ + Y+   P+ + K+
Sbjct: 148 LKEAIKGSKIAIVLLSRKYASSSWCLDELAEIMKCR-QMVGQIVMTIFYE-VDPTDIKKQ 207

Query: 124 LQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNL 183
             +FGK                             F K+   K +E              
Sbjct: 208 TGEFGK----------------------------AFTKTCRGKPKE-------------- 267

Query: 184 FSDQDNSNVLMEVPRWKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGV------- 243
                      +V RW+ ALE +  +    + +    +E+D+I+++   ++ +       
Sbjct: 268 -----------QVERWRKALEDVATIAG--YHSHSWRNEADMIEKISTDVSNMLNSFTPS 327

Query: 244 KESSKLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNLQPKF----- 303
           ++   LVG+   +  ++ LL L  +++  IGI G  GIGKTTIA+ L+  +  +F     
Sbjct: 328 RDFDGLVGMRAHMDMLEQLLRLDLDEVRMIGIWGPPGIGKTTIARFLFNQVSDRFQLSAI 387

Query: 304 ------CH-NHCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVL 363
                 C+   CF   S Q  + +QNQ+L ++    + +I +      + ++ LR+KKV 
Sbjct: 388 MVNIKGCYPRPCFDEYSAQ--LQLQNQMLSQMINHKDIMISH----LGVAQERLRDKKVF 447

Query: 364 IVLDGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSA 423
           +VLD VD+  QL+ L     WF  GSRII+TT +  + +     H    Y +E+  +D A
Sbjct: 448 LVLDEVDQLGQLDALAKETRWFGPGSRIIITTEDLGVLKAHGINH---VYKVEYPSNDEA 507

Query: 424 FSLFCNHAFG--EDHEPPDE---------------------------------------- 483
           F +FC +AFG  + HE  DE                                        
Sbjct: 508 FQIFCMNAFGQKQPHEGFDEIAWEVTCLAGELPLGLKVLGSALRGKSKREWERTLPRLKT 567

Query: 484 ---------------------------------------------NFRSSYNKLHLLAER 543
                                                         F      LHLLA++
Sbjct: 568 SLDGKIGSIIQFSYDVLCDEDKYLFLYIACLFNGESTTKVKELLGKFLDVKQGLHLLAQK 627

Query: 544 NLIQISNENVHMHTLVRCMGRGIAR------------------------EERETQSRIWL 603
           +LI    E +HMHTL+   GR  +R                        ++  T SR ++
Sbjct: 628 SLISFDGERIHMHTLLEQFGRETSRKQFVHHGFTKRQLLVGARGICEVLDDDTTDSRRFI 687

Query: 604 ------------------------------------------------------------ 663
                                                                       
Sbjct: 688 GIHLELSNTEEELNISEKVLERVHDFHFVRIDASFQPERLQLALQDLIYHSPKIRSLNWY 747

Query: 664 ----------------------RKDIRRVFHQEKEFSWLKEIDVSGSENLIETPDFSKVP 723
                                   ++R+++   K+   LK +D+S S  L E P+ S   
Sbjct: 748 GYESLCLPSTFNPEFLVELDMRSSNLRKLWEGTKQLRNLKWMDLSYSSYLKELPNLSTAT 807

Query: 724 NLQRLILRNCERLRMVHPSISTLNHLVSLDM---AGLKSFPEIEKHTKSNLTELHLDGTL 761
           NL+ L LRNC  L  +  SI  L  L  LD+   + L+  P IE  TK    +L    +L
Sbjct: 808 NLEELKLRNCSSLVELPSSIEKLTSLQILDLENCSSLEKLPAIENATKLRELKLQNCSSL 867

BLAST of Lsi08G001100 vs. TAIR 10
Match: AT5G44510.1 (target of AVRB operation1 )

HSP 1 Score: 171.0 bits (432), Expect = 5.7e-42
Identity = 224/913 (24.53%), Postives = 378/913 (41.40%), Query Frame = 0

Query: 2   NPPSSSSFPSMKYDVFISFRGGGDDDKFTCQLAEELSNNGLTTFMSNDRFKFGDDSENIV 61
           +PPSS S  +  + VF+SFRG          + +E   NG+T F+ N+  + G     ++
Sbjct: 29  SPPSSLS-QNWLHPVFLSFRGEDVRKGLLSHIQKEFQRNGITPFIDNEMKRGGSIGPELL 88

Query: 62  QEAIILNSRYLVVVLTKDYITSIRCLRELSKIMEYRGEDYEEVVIPVLYQHHHPSCLVKE 121
           Q   I  S+  +++L+++Y +S  CL EL +IM+ R E+  + V+ V Y    PS + K+
Sbjct: 89  Q--AIRGSKIAIILLSRNYGSSKWCLDELVEIMKCR-EELGQTVMTVFYD-VDPSDVRKQ 148

Query: 122 LQDFGKRLEDYKVRSKEGRFFKSNCGFLKTKLVAKFNKSFSTKREETDLELNLVQHDPNL 181
             DFGK              FK  C     ++V ++ ++ ++                  
Sbjct: 149 KGDFGK-------------VFKKTCVGRPEEMVQRWKQALTSA----------------- 208

Query: 182 FSDQDNSNVLMEVPR-WKSALEGITNLPTTIFLTEIRSHESDIIKEVKEIINGV--KESS 241
                 +N+L E  R W++  + I                  I K+V ++++    K+  
Sbjct: 209 ------ANILGEDSRNWENEADMIIK----------------ISKDVSDVLSFTPSKDFD 268

Query: 242 KLVGISPQLHQMDNLLDLGSNDICFIGIVGMGGIGKTTIAKALYKNL------------- 301
           + VGI     ++ +LL L   ++  IGI G  GIGKTTI++ LY  L             
Sbjct: 269 EFVGIEAHTTEITSLLQLDLEEVRMIGIWGPAGIGKTTISRVLYNKLFHQFQLGAIIDNI 328

Query: 302 ---QPKFCHNHCFLRVSGQKLVSMQNQLLLRLCGKLEEVIKNEDHGAQLIKKCLRNKKVL 361
               P+ CH+    +      + +Q +LL ++  + + V+ +      + ++ L++KKVL
Sbjct: 329 KVRYPRPCHDEYSAK------LQLQKELLSQMINQKDMVVPH----LGVAQERLKDKKVL 388

Query: 362 IVLDGVDEKRQLEKLVGSPDWFSSGSRIIVTTRNRNLCRQPKYKHKIFEYDIEFLDHDSA 421
           +VLD VD   QL+ +     WF  GSRIIV T++  L +    K   + Y ++F   D A
Sbjct: 389 LVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQDLKLLKAHGIK---YIYKVDFPTSDEA 448

Query: 422 FSLFCNHAFGED----------------------------------------------HE 481
             +FC +AFGE                                                 
Sbjct: 449 LEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLRVMGSYLRRMSKQEWAKSIPRLRT 508

Query: 482 PPDENFRS----SYNK--------------------------------------LHLLAE 541
             D++  S    SYN                                       L +LA+
Sbjct: 509 SLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERIETLEVFLAKKSVDMRQGLQILAD 568

Query: 542 RNLIQISNENVHMHTLVRCMGRGIAREE--RETQSRIWL--RKDIRRVFHQEKEFSWLKE 601
           ++L+ ++  N+ MH L+  +G  I R++   +   R +L   +DI  V   +     L  
Sbjct: 569 KSLLSLNLGNIEMHNLLVQLGLDIVRKQSIHKPGKRQFLVDTEDICEVLTDDTGTRTLIG 628

Query: 602 ID------VSGSENLIETPDFSKVPNLQRLILRN-------------------CERLRMV 661
           ID      + G  N+ E   F ++ NLQ L   +                     +LR++
Sbjct: 629 IDLELSGVIEGVINISERA-FERMCNLQFLRFHHPYGDRCHDILYLPQGLSHISRKLRLL 688

Query: 662 H---------PSISTLNHLVSLDM--------------------------AGLKSFPEIE 721
           H         P       LV ++M                            LK  P+  
Sbjct: 689 HWERYPLTCLPPKFNPEFLVKINMRDSMLEKLWDGNEPIRNLKWMDLSFCVNLKELPDFS 748

Query: 722 KHTKSNLTELHLDGTL-IKDLPFSLGLLTHLALLNLRDCTKLSSLPRSIRKLISLQTLNL 742
             T +NL EL L   L + +LP S+G  T+L  L+L DC+ L  LP SI  L +L+ L L
Sbjct: 749 --TATNLQELRLINCLSLVELPSSIGNATNLLELDLIDCSSLVKLPSSIGNLTNLKKLFL 808